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Pesquisa : D01.268.150 [Categoria DeCS]
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Id: biblio-1087631
Autor: Yuan, Wei; Lin, Xiaoqiong; Zhong, Shuang; Chen, Junren; Wang, Zhao; Sun, Jie.
Título: Enhanced pyruvic acid yield in an osmotic stress-resistant mutant of Yarrowia lipolytica
Fonte: Electron. j. biotechnol;44:19-24, Mar. 2020. ilus, graf.
Idioma: en.
Projeto: Zhejiang Provincial Natural Science Foundation of China; . National Natural Science Foundation of China.
Resumo: BACKGROUND: Pyruvic acid (PA), a vital α-oxocarboxylic acid, plays an important role in energy and carbon metabolism. The oleaginous yeast Yarrowia lipolytica (Y. lipolytica) has considerable potential for the production of PA. An increased NaCl concentration reportedly increases the biomass and PA yield of Y. lipolytica. RESULTS: To increase the yield of PA, the NaCl-tolerant Y. lipolytica A4 mutant was produced using the atmospheric and room temperature plasma method of mutation. The A4 mutant showed growth on medium containing 160 g/L NaCl. The PA yield of the A4 mutant reached 97.2 g/L at 120 h (0.795 g/g glycerol) in a 20-L fermenter with glycerol as the sole carbon source, which was 28.9% higher than that of the parental strain. CONCLUSION: The PA yield from Y. lipolytica can be improved by increasing its NaCl tolerance.
Descritores: Ácido Pirúvico/metabolismo
Yarrowia/genética
Yarrowia/metabolismo
-Pressão Osmótica
Leveduras
Carbono/metabolismo
Cloreto de Sódio
Reatores Biológicos
Tolerância ao Sal/genética
Fermentação
Glicerol/metabolismo
Mutação
Responsável: CL1.1 - Biblioteca Central


  2 / 198 LILACS  
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Id: biblio-839350
Autor: Cardoso, Aline Assis; Andraus, Michel de Paula; Borba, Tereza Cristina de Oliveira; Martin-Didonet, Claudia Cristina Garcia; Ferreira, Enderson Petrônio de Brito.
Título: Characterization of rhizobia isolates obtained from nodules of wild genotypes of common bean
Fonte: Braz. j. microbiol;48(1):43-50, Jan.-Mar. 2017. tab, graf.
Idioma: en.
Resumo: Abstract This study aimed to evaluate the tolerance to salinity and temperature, the genetic diversity and the symbiotic efficiency of rhizobia isolates obtained from wild genotypes of common bean cultivated in soil samples from the States of Goiás, Minas Gerais and Paraná. The isolates were subjected to different NaCl concentrations (0%, 1%, 2%, 4% and 6%) at different temperatures (28 °C, 33 °C, 38 °C, 43 °C and 48 °C). Genotypic characterization was performed based on BOX-PCR, REP-PCR markers and 16S rRNA sequencing. An evaluation of symbiotic efficiency was carried out under greenhouse conditions in autoclaved Leonard jars. Among 98 isolates about 45% of them and Rhizobium freirei PRF81 showed a high tolerance to temperature, while 24 isolates and Rhizobium tropici CIAT899 were able to use all of the carbon sources studied. Clustering analysis based on the ability to use carbon sources and on the tolerance to salinity and temperature grouped 49 isolates, R. tropici CIAT899 and R. tropici H12 with a similarity level of 76%. Based on genotypic characterization, 65% of the isolates showed an approximately 66% similarity with R. tropici CIAT899 and R. tropici H12. About 20% of the isolates showed symbiotic efficiency similar to or better than the best Rhizobium reference strain (R. tropici CIAT899). Phylogenetic analysis of the 16S rRNA revealed that two efficient isolates (ALSG5A1 and JPrG6A8) belong to the group of strains used as commercial inoculant for common bean in Brazil and must be assayed in field experiments.
Descritores: Rhizobium/fisiologia
Simbiose
Phaseolus/genética
Phaseolus/microbiologia
Nódulos Radiculares de Plantas/microbiologia
Genótipo
-Filogenia
Rhizobium/isolamento & purificação
Rhizobium/classificação
Adaptação Biológica
Carbono/metabolismo
RNA Ribossômico 16S/genética
Phaseolus/classificação
Meio Ambiente
Tolerância ao Sal
Responsável: BR1.1 - BIREME


  3 / 198 LILACS  
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Id: biblio-974323
Autor: Wang, Xiuwen; Zhang, Xiaohua; Yao, Qingshou; Hua, Dongliang; Qin, Jiayang.
Título: Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol
Fonte: Braz. j. microbiol;49(supl.1):160-165, 2018. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Shandong Province Science and Technology Project; . Jinan Youth Science and Technology Star Project.
Resumo: Abstract Sclareol is an important intermediate for ambroxide synthesis industries. Hyphozyma roseonigra ATCC 20624 was the only reported strain capable of degrading sclareol to the main product of sclareol glycol, which is the precursor of ambroxide. To date, knowledge is lacking about the effects of sclareol on cells and the proteins involved in sclareol metabolism. Comparative proteomic analyses were conducted on the strain H. roseonigra ATCC 20624 by using sclareol or glucose as the sole carbon source. A total of 79 up-regulated protein spots with a >2.0-fold difference in abundance on 2-D gels under sclareol stress conditions were collected for further identification. Seventy spots were successfully identified and finally integrated into 30 proteins. The up-regulated proteins under sclareol stress are involved in carbon metabolism; and nitrogen metabolism; and replication, transcription, and translation processes. Eighteen up-regulated spots were identified as aldehyde dehydrogenases, which indicating that aldehyde dehydrogenases might play an important role in sclareol metabolism. Overall, this study may lay the fundamentals for further cell engineering to improve sclareol glycol production.
Descritores: Ascomicetos/metabolismo
Proteínas Fúngicas/metabolismo
Diterpenos/metabolismo
-Ascomicetos/genética
Ascomicetos/química
Proteínas Fúngicas/química
Carbono/metabolismo
Eletroforese em Gel Bidimensional
Regulação Fúngica da Expressão Gênica
Proteômica
Glucose/metabolismo
Responsável: BR1.1 - BIREME


  4 / 198 LILACS  
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Id: biblio-974317
Autor: Zepeda, Andrea B; Pessoa Jr, Adalberto; Farías, Jorge G.
Título: Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast
Fonte: Braz. j. microbiol;49(supl.1):119-127, 2018. tab, graf.
Idioma: en.
Projeto: Comisión Nacional de Investigación Científica y Tecnológica de Chile; . AZP; . FAPESP.
Resumo: Abstract Nowadays, it is necessary to search for different high-scale production strategies to produce recombinant proteins of economic interest. Only a few microorganisms are industrially relevant for recombinant protein production: methylotrophic yeasts are known to use methanol efficiently as the sole carbon and energy source. Pichia pastoris is a methylotrophic yeast characterized as being an economical, fast and effective system for heterologous protein expression. Many factors can affect both the product and the production, including the promoter, carbon source, pH, production volume, temperature, and many others; but to control all of them most of the time is difficult and this depends on the initial selection of each variable. Therefore, this review focuses on the selection of the best promoter in the recombination process, considering different inductors, and the temperature as a culture medium variable in methylotrophic Pichia pastoris yeast. The goal is to understand the effects associated with different factors that influence its cell metabolism and to reach the construction of an expression system that fulfills the requirements of the yeast, presenting an optimal growth and development in batch, fed-batch or continuous cultures, and at the same time improve its yield in heterologous protein production.
Descritores: Pichia/genética
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/genética
Carbono/metabolismo
Regiões Promotoras Genéticas
-Pichia/crescimento & desenvolvimento
Pichia/metabolismo
Temperatura
Microbiologia Industrial
Responsável: BR1.1 - BIREME


  5 / 198 LILACS  
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Id: biblio-974291
Autor: Agunbiade, Mayowa; Pohl, Carolina; Ashafa, Omotayo.
Título: Bioflocculant production from Streptomyces platensis and its potential for river and waste water treatment
Fonte: Braz. j. microbiol;49(4):731-741, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT A bacterium isolated from Sterkfontein dam was confirmed to produce bioflocculant with excellent flocculation activity. The 16S rDNA nucleotide sequence analyses revealed the bacteria to have 99% similarity to Streptomyces platensis strain HBUM174787 and the sequence was deposited in the Genbank as Streptomyces platensis with accession number FJ 486385.1. Culture conditions for optimal production of the bioflocculant included glucose as a sole carbon source, resulting in flocculating activity of 90%. Other optimal conditions included: peptone as nitrogen source; presence of Mg2+ as cations and inoculum size of 1.0% (v/v) at neutral pH of 7. Optimum dose of the purified bioflocculant for the clarification of 4 g/L kaolin clay suspension at neutral pH was 0.2 mg/mL. Energy Dispersive X-ray analysis confirmed elemental composition of the purified bioflocculant in mass proportion (%w/w): carbon (21.41), oxygen (35.59), sulphur (26.16), nitrogen (0.62) and potassium (7.48). Fourier Transform Infrared Spectroscopy (FTIR) indicated the presence of hydroxyl, carboxyl, methoxyl and amino group in the bioflocculant. The bioflocculant produced by S. platensis removed chemical oxygen demand (COD) in river water and meat processing wastewater at efficiencies of 63.1 and 46.6% respectively and reduced their turbidity by 84.3 and 75.6% respectively. The high flocculating rate and removal efficiencies displayed by S. platensis suggests its industrial application in wastewater treatment.
Descritores: Streptomyces/química
Proteínas de Bactérias/metabolismo
Águas Residuárias/química
-Streptomyces/isolamento & purificação
Streptomyces/genética
Streptomyces/metabolismo
Proteínas de Bactérias/genética
Microbiologia da Água
Carbono/metabolismo
Purificação da Água
Rios/química
Floculação
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  6 / 198 LILACS  
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Id: biblio-974282
Autor: Santos, Alexandra de Andrade; Silveira, Joaquim Albenísio Gomes da; Guilherme, Eliezer de Araujo; Bonifacio, Aurenivia; Rodrigues, Artenisa Cerqueira; Figueiredo, Márcia do Vale Barreto.
Título: Changes induced by co-inoculation in nitrogen-carbon metabolism in cowpea under salinity stress
Fonte: Braz. j. microbiol;49(4):685-694, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT To mitigate the deleterious effects of abiotic stress, the use of plant growth-promoting bacteria along with diazotrophic bacteria has been increasing. The objectives of this study were to investigate the key enzymes related to nitrogen and carbon metabolism in the biological nitrogen fixation process and to elucidate the activities of these enzymes by the synergistic interaction between Bradyrhizobium and plant growth-promoting bacteria in the absence and presence of salt stress. Cowpea plants were cultivated under axenic conditions, inoculated with Bradyrhizobium and co-inoculated with Bradyrhizobium sp. and Actinomadura sp., Bradyrhizobium sp. and Bacillus sp., Bradyrhizobium sp. and Paenibacillus graminis, and Bradyrhizobium sp. and Streptomycessp.; the plants were also maintained in the absence (control) and presence of salt stress (50 mmolL-1 NaCl). Salinity reduced the amino acids, free ammonia, ureides, proteins and total nitrogen content in nodules and increased the levels of sucrose and soluble sugars. The co-inoculations responded differently to the activity of glutamine synthetase enzymes under salt stress, as well as glutamate synthase, glutamate dehydrogenase aminating, and acid invertase in the control and salt stress. Considering the development conditions of this experiment, co-inoculation with Bradyrhizobium sp. and Bacillus sp. in cowpea provided better symbiotic performance, mitigating the deleterious effects of salt stress.
Descritores: Carbono/metabolismo
Cloreto de Sódio/metabolismo
Vigna/metabolismo
Nitrogênio/metabolismo
-Microbiologia do Solo
Cloreto de Sódio/análise
Actinobacteria/fisiologia
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/metabolismo
Raízes de Plantas/microbiologia
Bradyrhizobium/fisiologia
Inoculantes Agrícolas/fisiologia
Vigna/crescimento & desenvolvimento
Vigna/microbiologia
FRONTAL LOBE0
Aminoácidos/metabolismo
Fixação de Nitrogênio
Responsável: BR1.1 - BIREME


  7 / 198 LILACS  
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Teixeira, Maria Francisca Simas
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Id: lil-788950
Autor: Kirsch, Larissa de Souza; Macedo, Ana Júlia Porto de; Teixeira, Maria Francisca Simas.
Título: Production of mycelial biomass by the Amazonian edible mushroom Pleurotus albidus
Fonte: Braz. j. microbiol;47(3):658-664, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT Edible mushroom species are considered as an adequate source of food in a healthy diet due to high content of protein, fiber, vitamins, and a variety of minerals. The representatives of Pleurotus genus are characterized by distinct gastronomic, nutritional, and medicinal properties among the edible mushrooms commercialized worldwide. In the present study, the growth of mycelial biomass of Pleurotus albidus cultivated in submerged fermentation was evaluated. Saccharose, fructose, and maltose were the three main carbon sources for mycelial biomass formation with corresponding yields of 7.28 g L−1, 7.07 g L−1, and 6.99 g L−1. Inorganic nitrogen sources did not stimulate growth and the optimal yield was significantly higher with yeast extract (7.98 g L−1). The factorial design used to evaluate the influence of saccharose and yeast extract concentration, agitation speed, and initial pH indicated that all variables significantly influenced the production of biomass, especially the concentration of saccharose. The greater amount of saccharose resulted in the production of significantly more biomass. The highest mycelial biomass production (9.81 g L−1) was reached in the medium formulated with 30.0 g L−1 saccharose, 2.5 g L−1 yeast extract, pH 7.0, and a speed of agitation at 180 rpm. Furthermore, P. albidus manifested different aspects of morphology and physiology under the growth conditions employed. Media composition affected mycelial biomass production indicating that the diversification of carbon sources promoted its improvement and can be used as food or supplement.
Descritores: Biomassa
Pleurotus/fisiologia
Micélio
Fermentação
-Carbono/metabolismo
Pleurotus/ultraestrutura
Metabolismo dos Carboidratos
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  8 / 198 LILACS  
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Id: biblio-828208
Autor: Munaganti, Rajesh Kumar; Muvva, Vijayalakshmi; Konda, Saidulu; Naragani, Krishna; Mangamuri, Usha Kiranmayi; Dorigondla, Kumar Reddy; Akkewar, Dattatray. M.
Título: Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards
Fonte: Braz. j. microbiol;47(4):1030-1038, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract An actinobacterial strain VL-RK_09 having potential antimicrobial activities was isolated from a mango orchard in Krishna District, Andhra Pradesh (India) and was identified as Arthrobacter kerguelensis. The strain A. kerguelensis VL-RK_09 exhibited a broad spectrum of in vitro antimicrobial activity against bacteria and fungi. Production of bioactive metabolites by the strain was the highest in modified yeast extract malt extract dextrose broth, as compared to other media tested. Lactose (1%) and peptone (0.5%) were found to be the most suitable carbon and nitrogen sources, respectively, for the optimum production of the bioactive metabolites. The maximum production of the bioactive metabolites was detected in the culture medium with an initial pH of 7, in which the strain was incubated for five days at 30 °C under shaking conditions. Screening of secondary metabolites obtained from the culture broth led to the isolation of a compound active against a wide variety of Gram-positive and negative bacteria and fungi. The structure of the first active fraction was elucidated using Fourier transform infrared spectroscopy, electrospray ionization mass spectrometry, 1H and 13C nuclear magnetic resonance spectroscopy. The compound was identified as S,S-dipropyl carbonodithioate. This study is the first report of the occurrence of this compound in the genus Arthrobacter.
Descritores: Arthrobacter/isolamento & purificação
Arthrobacter/metabolismo
Mangifera/microbiologia
Anti-Infecciosos/metabolismo
Anti-Infecciosos/farmacologia
-Sais/metabolismo
Temperatura
Carbono/metabolismo
Testes de Sensibilidade Microbiana
Metaboloma
Metabolômica/métodos
Concentração de Íons de Hidrogênio
Anti-Infecciosos/isolamento & purificação
Anti-Infecciosos/química
Minerais/metabolismo
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  9 / 198 LILACS  
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Id: biblio-828205
Autor: Aliabadi, Nasrin; Aminzadeh, Saeed; Karkhane, Ali Asghar; Haghbeen, Kamahldin.
Título: Thermostable chitinase from Cohnella sp. A01: isolation and product optimization
Fonte: Braz. j. microbiol;47(4):931-940, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Projeto: International Foundation for Science; . Committee on Scientific and Technological Cooperation; . National Institute of Genetic Engineering and Biotechnology.
Resumo: Abstract Twelve bacterial strains isolated from shrimp farming ponds were screened for their growth activity on chitin as the sole carbon source. The highly chitinolytic bacterial strain was detected by qualitative cup plate assay and tentatively identified to be Cohnella sp. A01 based on 16S rDNA sequencing and by matching the key morphological, physiological, and biochemical characteristics. The cultivation of Cohnella sp. A01 in the suitable liquid medium resulted in the production of high levels of enzyme. The colloidal chitin, peptone, and K2HPO4 represented the best carbon, nitrogen, and phosphorus sources, respectively. Enzyme production by Cohnella sp. A01 was optimized by the Taguchi method. Our results demonstrated that inoculation amount and temperature of incubation were the most significant factors influencing chitinase production. From the tested values, the best pH/temperature was obtained at pH 5 and 70 °C, with Km and V max values of chitinase to be 5.6 mg/mL and 0.87 µmol/min, respectively. Ag+, Co2+, iodoacetamide, and iodoacetic acid inhibited the enzyme activity, whereas Mn2+, Cu2+, Tweens (20 and 80), Triton X-100, and EDTA increased the same. In addition, the study of the morphological alteration of chitin treated by enzyme by SEM revealed cracks and pores on the chitin surface, indicating a potential application of this enzyme in several industries.
Descritores: Bacillus/metabolismo
Quitinases/metabolismo
-Fósforo/metabolismo
Temperatura
Bacillus/isolamento & purificação
Bacillus/genética
Bacillus/ultraestrutura
Estabilidade Enzimática/efeitos dos fármacos
Carbono/metabolismo
RNA Ribossômico 16S/genética
Cinética
Quitinases/química
Análise de Sequência de DNA
Ativação Enzimática
Concentração de Íons de Hidrogênio
Íons
Metais
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  10 / 198 LILACS  
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Id: lil-788953
Autor: Nwinyi, Obinna C; Ajayi, Oluseyi O; Amund, Olukayode O.
Título: Degradation of polynuclear aromatic hydrocarbons by two strains of Pseudomonas
Fonte: Braz. j. microbiol;47(3):551-562, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT The goal of this investigation was to isolate competent polynuclear aromatic hydrocarbons degraders that can utilize polynuclear aromatic hydrocarbons of former industrial sites at McDoel Switchyard in Bloomington, Indiana. Using conventional enrichment method based on soil slurry, we isolated, screened and purified two bacterial species strains PB1 and PB2. Applying the ribotyping technique using the 16S rRNA gene analysis, the strains were assigned to the genus Pseudomonas (Pseudomonas plecoglossicida strain PB1 and Pseudomonas sp. PB2). Both isolates showed promising metabolic capacity on pyrene sprayed MS agar plates during the preliminary investigations. Using time course studies in the liquid cultures at calculated concentrations 123, 64, 97 and 94 ppm for naphthalene, chrysene, fluroanthene and pyrene, P. plecoglossicida strain PB1 and Pseudomonas sp. PB2 showed partial utilization of the polynuclear aromatic hydrocarbons. Naphthalene was degraded between 26% and 40%, chrysene 14% and 16%, fluroanthene 5% and 7%; pyrene 8% and 13% by P. plecoglossicida strain PB1 and Pseudomonas sp. PB2 respectively. Based on their growth profile, we developed a model R2 = 1 to predict the degradation rate of slow polynuclear aromatic hydrocarbon-degraders where all the necessary parameters are constant. From this investigation, we confirm that the former industrial site soil microbial communities may be explored for the biorestoration of the industrial site.
Descritores: Hidrocarbonetos Policíclicos Aromáticos/metabolismo
Pseudomonas/metabolismo
-Filogenia
Pseudomonas/classificação
Pseudomonas/genética
Pirenos/metabolismo
Solo/química
Microbiologia do Solo
Biodegradação Ambiental
Carbono/química
RNA Ribossômico 16S/genética
Crisenos/metabolismo
Naftalenos/metabolismo
Nitrogênio/química
Responsável: BR1.1 - BIREME



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