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Id: biblio-950718
Autor: Acharya, Arpan; Vaniawala, Salil; Shah, Parth; Parekh, Harsh; Misra, Rabindra Nath; Wani, Minal; Mukhopadhyaya, Pratap N.
Título: A robust HIV-1 viral load detection assay optimized for Indian sub type C specific strains and resource limiting setting
Fonte: Biol. Res;47:1-8, 2014. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Human Immunodeficiency Virus Type 1 (HIV-1) viral load testing at regular intervals is an integral component of disease management in Acquired Immunodeficiency Syndrome (AIDS) patients. The need in countries like India is therefore an assay that is not only economical but efficient and highly specific for HIV-1 sub type C virus. This study reports a SYBR Green-based HIV-1 real time PCR assay for viral load testing and is designed for enhanced specificity towards HIV-1 sub type C viruses prevalent in India. RESULTS: Linear regression of the observed and reference concentration of standards used in this study generated a correlation coefficient of 0.998 (p<0.001). Lower limit of detection of the test protocol was 50 copies/ml of plasma. The assay demonstrated 100% specificity when tested with negative control sera. The Spearman coefficient of the reported assay with an US-FDA approved, Taqman probe-based commercial kit was found to be 0.997. No significant difference in viral load was detected when the SYBR Green based assay was used to test infected plasma stored at -20°C and room temperature for 7 days respectively (Wilcoxon signed rank test, p=0.105). In a comparative study on 90 pretested HIV-1 positive samples with viral loads ranging from 5,000 - 25,000 HIV-1 RNA copies/ml and between two commercial assays it was found that the later failed to amplify in 13.33% and 10% samples respectively while in 7.77% and 4.44% samples the copy number values were reduced by >0.5 log value, a figure that is considered clinically significant by physicians. CONCLUSION: The HIV-1 viral load assay reported in this study was found to be robust, reliable, economical and effective in resource limited settings such as those existing in India. PCR probes specially designed from HIV-1 Subtype C-specific nucleotide sequences originating from India imparted specificity towards such isolates and demonstrated superior results when compared to two similar commercial assays widely used in India.
Descritores: RNA Viral/sangue
Infecções por HIV/diagnóstico
HIV-1/isolamento & purificação
Carga Viral/métodos
-Compostos Orgânicos
Kit de Reagentes para Diagnóstico/economia
Sequência de Bases/genética
Genes gag/genética
Modelos Lineares
Sensibilidade e Especificidade
HIV-1/classificação
Estatísticas não Paramétricas
Gerenciamento Clínico
Limite de Detecção
Reação em Cadeia da Polimerase em Tempo Real
Invenções
Índia
Limites: Humanos
Tipo de Publ: Estudo de Validação
Responsável: CL1.1 - Biblioteca Central


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Id: lil-785796
Autor: Romeiro, Marilia Farignoli; Souza, William Marciel de; Tolardo, Aline Lavado; Vieira, Luiz Carlos; Colombo, Tatiana Elias; Aquino, Victor Hugo; Nogueira, Maurício Lacerda; Figueiredo, Luiz Tadeu Moraes.
Título: Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil
Fonte: Rev. Soc. Bras. Med. Trop;49(3):279-285tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo; . CNPq.
Resumo: Abstract: INTRODUCTION: The genus Flavivirus includes several pathogenic species that cause severe illness in humans. Therefore, a rapid and accurate molecular method for diagnosis and surveillance of these viruses would be of great importance. Here, we evaluate and optimize a quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) method for the diagnosis of the Flavivirus genus. METHODS: We evaluated different commercial kits that use the SYBR Green system for real-time RT-PCR with a primer set that amplifies a fragment of the NS5 flavivirus gene. The specificity and sensitivity of the assay were tested using twelve flaviviruses and ribonucleic acid (RNA) transcribed from the yellow fever virus. Additionally, this assay was evaluated using the sera of 410 patients from different regions of Brazil with acute febrile illness and a negative diagnosis for the dengue virus. RESULTS: The real-time RT-PCR amplified all flaviviruses tested at a melting temperature of 79.92 to 83.49°C. A detection limit of 100 copies per ml was determined for this assay. Surprisingly, we detected dengue virus in 4.1% (17/410) of samples from patients with febrile illness and a supposedly negative dengue infection diagnosis. The viral load in patients ranged from 2.1×107to 3.4×103copies per ml. CONCLUSIONS: The real-time RT-PCR method may be very useful for preliminary diagnoses in screenings, outbreaks, and other surveillance studies. Moreover, this assay can be easily applied to monitor viral activity and to measure viral load in pathogenesis studies.
Descritores: Infecções por Flavivirus/diagnóstico
Flavivirus/genética
-Compostos Orgânicos
Kit de Reagentes para Diagnóstico
Brasil
RNA Viral/genética
Sensibilidade e Especificidade
Infecções por Flavivirus/virologia
Primers do DNA
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Flavivirus/isolamento & purificação
Flavivirus/classificação
Corantes Fluorescentes
Limites: Humanos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-950749
Autor: Yu, Guangchao; Chen, Lei; Lin, Chii-wann; Li, Bing; Cui, Hemiao; Chen, Siyi; Miao, Jian; Bian, Huawei; Chen, Dingqiang; Deng, Yang.
Título: Loop-mediated isothermal amplification assays for screening of bacterial integrons
Fonte: Biol. Res;47:1-10, 2014. ilus, tab.
Idioma: en.
Projeto: National 973-Plan of China; . International Science & Technology Cooperation Program; . Science and Technology Planning Project of Guangdong Province, China; . National Natural Science Foundation of China; . National Science and Technology Support Program; . National Outstanding Doctoral Dissertation Funding; . Guangdong Outstanding Doctoral Dissertation Funding; . China Postdoctoral Science Foundation funded; . Fundamental Research Funds for the Central Universities; . Fund for Outstanding Youth of Anhui Academy of Agricultural Sciences.
Resumo: BACKGROUND: The occurrence and prevalence of integrons in clinical microorganisms and their role played in antimicrobial resistance have been well studied recently. As screening and detection of integrons are concerned, current diagnostic methodologies are restricted by significant drawbacks and novel methods are required for integrons detection. RESULTS: In this study, three loop-mediated isothermal amplification (LAMP) assays targeting on class 1, 2 and 3 integrons were implemented and evaluated. Optimization of these detection assays were performed, including studing on the reaction temperature, volume, time, sensitivity and specificity (both primers and targets). Application of the established LAMP assays were further verified on a total of 1082 isolates (previously identified to be 397 integron-positive and 685 integron-negative strains). According to the results, the indispensability of each primer had been confirmed and the optimal reaction temperature, volume and time were found to be 65°C, 45 min and 25 µL, respectively. As application was concerned, 361, 28 and 8 isolates carrying intI1, intI2 and intI3 yielded positive amplicons, respectively. Other 685 integron-negative bacteria were negative for the integron-screening LAMP assays, totaling the detection rate and specificity to be 100%. CONCLUSIONS: The intI1-, intI2- and intI3-LAMP assays established in this study were demonstrated to be the valid and rapid detection methodologies for the screening of bacterial integrons.
Descritores: DNA Bacteriano/isolamento & purificação
Técnicas de Amplificação de Ácido Nucleico/métodos
Integrons
-Compostos Orgânicos
Salmonella/genética
Serratia marcescens/genética
Staphylococcus/genética
Vibrio cholerae/genética
Contagem de Colônia Microbiana
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase/métodos
Sensibilidade e Especificidade
DNA Complementar
Primers do DNA
Integrases/genética
Farmacorresistência Bacteriana/genética
Eletroforese em Gel de Ágar
Escherichia coli/genética
Corantes Fluorescentes
Temperatura Alta
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1130150
Autor: Mariano, Lilian Gonçalves; Carvalho, Acácio Geraldo de; Trevisan, Henrique; Fernandes, Maria do Carmo Araújo.
Título: Identification and control of wood-deteriorating fungi / Identificação e controle de fungos deterioradores de madeira
Fonte: Arq. Inst. Biol;87:e0082020, 2020. ilus.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Resumo: Wood is rich in organic compounds; thus, it is susceptible to attacks by several deteriorating agents because they acknowledge such attacks as a necessary energy supply. Fungi stand out among these agents since they can attack the wood in trees (before felling) and in several post-felling stages. Fungi are biological agents that use organic compounds as food sources, and that is the reason why they degrade most chemical components found in wood. Wood attacked by fungi suffers significant value loss, since these degrading agents affect their mechanical and aesthetic properties. Fungi ­ which are classified as staining, molding and rotting ­ are one of the main responsible for the biggest losses in the timber industry. Wood can be virtually used in a whole range of environments, a fact that makes it susceptible to attacks by different fungal species, since the ideal conditions for fungal development change from species to species. In other words, the fact that one or more fungal species are capable of deteriorating wood is directly related to conditions wood is subjected to. Thus, it is essential knowing how to evaluate fungal attacks, symptoms to be taken into consideration at the time to identify the type of attacking organism and the attack stage, as well as the control and prevention measures to be applied to these organisms.(AU)

Rica em compostos orgânicos, a madeira é suscetível ao ataque de diversos agentes deterioradores por ser reconhecida como suprimento necessário de energia. Entre esses agentes, destacam-se os fungos, cujo início de ataque pode se dar ainda na árvore, antes do abate e nas diversas fases posteriores. Os fungos são agentes biológicos que necessitam de compostos orgânicos como fontes de alimento e, por essa razão, degradam grande parte dos componentes químicos da madeira. As madeiras, quando atacadas por fungos, sofrem grandes perdas de valor, afetando suas propriedades mecânicas e estéticas. Os fungos são considerados um dos principais responsáveis pelos maiores prejuízos na indústria madeireira e são classificados como manchadores, emboloradores e apodrecedores. Praticamente, a madeira pode estar em uso em uma variedade de ambientes; sabendo-se que as condições ideais de desenvolvimento de um fungo são diferentes para cada espécie, a madeira ficará suscetível ao ataque por diferentes espécies de fungos. Ou seja, o fato de uma ou mais espécies de fungos serem encontradas deteriorando a madeira está diretamente relacionado às condições em que ela se encontra. Dessa forma, torna-se importante saber como avaliar os ataques feitos por fungos, os sintomas a serem considerados para identificar o tipo de organismo e o estágio do ataque, bem como as medidas de controle e prevenção desses organismos.(AU)
Descritores: Madeira
Fungos
-Compostos Orgânicos
Árvores
Fatores Biológicos
Meio Ambiente
Abastecimento de Energia
Tipo de Publ: Revisão
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


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Id: lil-770924
Autor: García-Ubaque, César Augusto; García-Ubaque, Juan Carlos; Vaca-Bohórquez, Martha Lucía.
Título: Valoración económica en salud y medio ambiente del control de contaminantes orgánicos persistentes en Colombia / Economic assessment in health and environment from control of persistent organic pollutants in Colombia
Fonte: Rev. salud pública;17(6):1-1, nov.-dez. 2015. ilus, tab.
Idioma: es.
Resumo: Objetivo Estimar los beneficios económicos en salud y ambientales relacionados con la implementación del Convenio de Estocolmo para el control de Compuestos Orgánicos Persistentes (COP) en el país. Método La estimación se realizó teniendo en cuenta dos escenarios: cumplimiento del convenio y no cumplimiento del Convenio y el beneficio bruto se derivó de la diferencia en valor presente entre los costos en salud y ambientales que se asumen en cada escenario. Resultados Los beneficios brutos por la disminución de gastos en salud derivados de la implementación del Convenio se estiman entre USD $511 y USD $501 millones. Al introducir variables como los costos de gestión del convenio y los posibles beneficios por acceso a mercados internacionales, los beneficios para el país se estiman entre USD $1631 y USD $3118 millones. Discusión A pesar de los beneficios económicos generados por menores gastos en salud con la aplicación del Convenio, los costos asociados a la reducción de estos contaminantes, generan un balance negativo, que solo es compensado al cruzar estas cifras con la expectativa de mayores ingresos por acceso a mercados internacionales. Se considera que esta valoración económica como un primer ejercicio es un aporte importante, pero que debe ser actualizado a la luz de metodologías de valoración que involucren otras variables de rentabilidad social y diferentes escenarios por tecnologías emergentes, nuevo conocimiento sobre estos contaminantes, cambios en la legislación y/o cambios en las condiciones de tratados internacionales, entre otros.(AU)

Objective To estimate the economic benefits related to environment and health in the context of the implementation of the Stockholm Convention for the control of Persistent Organic Pollutants in the country. Method: The estimation was conducted based on two scenarios: non-compliance with the agreement and compliance with the Convention. Gross profit was derived from the difference in present value between the health and environmental costs that are assumed in each scenario. Results Gross profit by decreasing health costs arising from the implementation of the Convention was estimated at USD $ 511 and USD $ 501 million. By introducing variables such as management costs and agreement on potential benefits for access to international markets, the benefits to the country were estimated at between USD $1631 and USD $ 3118 million. Discussion Despite the economic benefits generated by lower expenditure on health for the Convention implementation, the costs associated with reducing pollutant emissions generated a negative balance, compensated only by the expectation of higher revenues for international market access. We consider this initial economic assessment an important contribution, but it should be reviewed to include valuation methodologies involving other social profitability variables and different scenarios for emerging technologies, new scientific knowledge about these pollutants, changes in legislation and / or changes in trade agreement conditions, among others.(AU)
Descritores: Compostos Orgânicos/normas
Formulação de Políticas
Poluição Ambiental/economia
-Indicadores Econômicos
Avaliação em Saúde/economia
Colômbia
Responsável: BR1.1 - BIREME


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Id: lil-332856
Autor: Vignola, Sílvia Regina do Amaral; Latorre, Willian César.
Título: Transgênicos: danos à saúde e ao meio ambiente / Transgenics: health and environment damages
Fonte: In: Marques, Maria Cristina; Diniz, Elizeu; Carvalho, Marisa Lima; Pereira, Sheila Duarte. Casos e fatos da vigilância sanitária sobre a saúde da sociedade. Säo Paulo, SOBRAVIME, 2002. p.211-223.
Idioma: pt.
Descritores: Fatores de Risco
Meio Ambiente
Compostos Orgânicos/toxicidade
Organismos Geneticamente Modificados
Saúde
-Vigilância Sanitária
Responsável: BR91.2 - Centro de Documentação
BR91.2; WA672, M357c, 2002


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Id: lil-242513
Autor: Freitas, Jefferson Benedito Pires de; Cosenza, Gilmar W.
Título: Grupo de poeiras e fibras no PCTBI: afecções respiratórias em trabalhadores em moihos de trigo / Dirties and fifers group at PCTBI: workers respiratories diseases at wheats guinders
Fonte: In: Säo Paulo (Estado). Secretaria da Saúde. Divisäo de Saúde do Trabalhador. Rede Especial - Revista do Projeto de Cooperaçäo Técnica Brasil-Itália: "Proteçäo à saúde nos ambientes de trabalho" - 1998. Säo Paulo, IMESP, 1998. p.99-104, ilus, tab.
Idioma: pt.
Conferência: Apresentado em: Seminário Internacional Brasil-Itália: Cooperaçäo Técnica em Saúde do Trabalhador no Estado de Säo Paulo, Säo Paulo, 1994-1998.
Descritores: Poeira/efeitos adversos
Doenças Respiratórias
Exposição Ocupacional/análise
Saúde do Trabalhador
-Triticum/efeitos adversos
Ambiente de Trabalho
Acidentes de Trabalho/classificação
Riscos Ambientais
Ruído Ocupacional/efeitos adversos
Medição de Risco
Compostos Orgânicos
Formulação de Projetos
Responsável: BR91.2 - Centro de Documentação
BR91.2; WA400, S239r


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Id: lil-242503
Autor: Lopes, Cleide; Paiva, Olga Ribas; Magnanelli, Neli Pires; Barancoski, Yaeko Oka; Franco, Maria Aparecida Juliano; Shiraishi, Nanci Yoriko.
Título: Estudo sobre a exposiçäo a solventes orgânicos em trabalhadores dos setores de pintura e contíguos em garagens de manutençäo de veículos em serviço de transporte / Organics solventes exposition study at workers of painting sectors and contiguos in vehicle maintenance garage at transportation service
Fonte: In: Säo Paulo (Estado). Secretaria da Saúde. Divisäo de Saúde do Trabalhador. Rede Especial - Revista do Projeto de Cooperaçäo Técnica Brasil-Itália: "Proteçäo à saúde nos ambientes de trabalho" - 1998. Säo Paulo, IMESP, 1998. p.39-41, graf.
Idioma: pt.
Conferência: Apresentado em: Seminário Internacional Brasil-Itália: Cooperaçäo Técnica em Saúde do Trabalhador no Estado de Säo Paulo, Säo Paulo, 1994-1998.
Descritores: Pintura/toxicidade
Solventes/análise
Ambiente de Trabalho
Exposição Ocupacional/análise
-Compostos Orgânicos/efeitos adversos
Responsável: BR91.2 - Centro de Documentação
BR91.2; WA400, S239r


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Id: biblio-1073025
Autor: Nascimento, Sebastião Dourival.
Título: A qualidade da água do abastecimento público na estância turistica de Pereira Barreto / The quality of the water of the public supplying in the tourist ranch of Barreto Pereira.
Fonte: Pereira Barreto; s.n; jul. 2000. 34 p. tab.
Idioma: pt.
Descritores: Abastecimento de Água
Características Organolépticas da Água
Compostos Inorgânicos
Compostos Orgânicos
Hidrogeologia
Qualidade da Água
Vigilância Sanitária
Água Subterrânea
-Abastecimento de Água/história
Saúde Pública
Responsável: BR91.2 - Centro de Documentação
BR91.2; WA686, N244q, 2000; BR1763.1


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Id: lil-788974
Autor: Gururaj, P; Ramalingam, Subramanian; Devi, Ganesan Nandhini; Gautam, Pennathur.
Título: Process optimization for production and purification of a thermostable, organic solvent tolerant lipase from Acinetobacter sp. AU07
Fonte: Braz. j. microbiol;47(3):647-657, July-Sept. 2016. tab, graf.
Idioma: en.
Projeto: Department of Biotechnology, Government of India.
Resumo: ABSTRACT The purpose of this study was to isolate, purify and optimize the production conditions of an organic solvent tolerant and thermostable lipase from Acinetobacter sp. AU07 isolated from distillery waste. The lipase production was optimized by response surface methodology, and a maximum production of 14.5 U/mL was observed at 30 ºC and pH 7, using a 0.5% (v/v) inoculum, 2% (v/v) castor oil (inducer), and agitation 150 rpm. The optimized conditions from the shake flask experiments were validated in a 3 L lab scale bioreactor, and the lipase production increased to 48 U/mL. The enzyme was purified by ammonium sulfate precipitation and ion exchange chromatography and the overall yield was 36%. SDS-PAGE indicated a molecular weight of 45 kDa for the purified protein, and Matrix assisted laser desorption/ionization time of flight analysis of the purified lipase showed sequence similarity with GDSL family of lipases. The optimum temperature and pH for activity of the enzyme was found to be 50 ºC and 8.0, respectively. The lipase was completely inhibited by phenylmethylsulfonyl fluoride but minimal inhibition was observed when incubated with ethylenediaminetetraacetic acid and dithiothreitol. The enzyme was stable in the presence of non-polar hydrophobic solvents. Detergents like SDS inhibited enzyme activity; however, there was minimal loss of enzyme activity when incubated with hydrogen peroxide, Tween 80 and Triton X-100. The kinetic constants (Km and Vmax) revealed that the hydrolytic activity of the lipase was specific to moderate chain fatty acid esters. The Vmax, Km and Vmax/Km ratio of the enzyme were 16.98 U/mg, 0.51 mM, and 33.29, respectively when 4-nitrophenyl palmitate was used as a substrate.
Descritores: Compostos Orgânicos
Solventes
Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/biossíntese
Acinetobacter/enzimologia
Lipase/isolamento & purificação
Lipase/biossíntese
-Compostos Orgânicos/química
Solventes/química
Especificidade por Substrato
Temperatura
Proteínas de Bactérias/química
Estabilidade Enzimática
Cinética
Cromatografia por Troca Iônica
Ativação Enzimática
Espaço Extracelular/enzimologia
Concentração de Íons de Hidrogênio
Íons
Lipase/química
Lipólise
Metais
Peso Molecular
Responsável: BR1.1 - BIREME



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