Base de dados : LILACS
Pesquisa : D02.033.160 [Categoria DeCS]
Referências encontradas : 3 [refinar]
Mostrando: 1 .. 3   no formato [Detalhado]

página 1 de 1

  1 / 3 LILACS  
              next record last record
para imprimir
Texto completo SciELO Brasil
Texto completo
Id: lil-741263
Autor: Harwani, Dharmesh.
Título: Regulation of gene expression: Cryptic β-glucoside (bgl) operon of Escherichia coli as a paradigm
Fonte: Braz. j. microbiol;45(4):1139-1144, Oct.-Dec. 2014. ilus.
Idioma: en.
Resumo: Bacteria have evolved various mechanisms to extract utilizable substrates from available resources and consequently acquire fitness advantage over competitors. One of the strategies is the exploitation of cryptic cellular functions encoded by genetic systems that are silent under laboratory conditions, such as the bgl (β-glucoside) operon of E. coli. The bgl operon of Escherichia coli, involved in the uptake and utilization of aromatic β-glucosides salicin and arbutin, is maintained in a silent state in the wild type organism by the presence of structural elements in the regulatory region. This operon can be activated by mutations that disrupt these negative elements. The fact that the silent bgl operon is retained without accumulating deleterious mutations seems paradoxical from an evolutionary view point. Although this operon appears to be silent, specific physiological conditions might be able to regulate its expression and/or the operon might be carrying out function(s) apart from the utilization of aromatic β-glucosides. This is consistent with the observations that the activated operon confers a Growth Advantage in Stationary Phase (GASP) phenotype to Bgl+ cells and exerts its regulation on at least twelve downstream target genes.
Descritores: Escherichia coli/enzimologia
Escherichia coli/genética
Regulação da Expressão Gênica
Álcoois Benzílicos/metabolismo
Escherichia coli/crescimento & desenvolvimento
Escherichia coli/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME

  2 / 3 LILACS  
              first record previous record next record last record
para imprimir
Texto completo SciELO Chile
Texto completo
Id: lil-668693
Autor: Geum-Hwa, Lee; Hyung-Ryong, Kim; Sang-Yong, Han; Bidur, Bhandary; Do-Sung, Kim; Min-Gul, Kim; Byung-Ok, So; Sun-Young, Kim; Kyu-Sik, Jo; Bo-Hee, Lee; Hee-Nam, Seo; Soo-Wan, Chae; Han-Jung, Chae.
Título: Gastrodia elata Blume and its pure compounds protect BV-2 microglial-derived cell lines against β-amyloid: The involvement of GRP78 and CHOP
Fonte: Biol. Res;45(4):403-410, 2012. ilus.
Idioma: en.
Projeto: funding from the MooJoo Hyangto Research Group.
Resumo: Objectives: Gastrodia elata (GE) Blume (Orchidaceae) has been previously known for its therapeutic benefits against neurodegenerative diseases. Microglial activation and death have been implicated in the pathogenesis of a variety of neurodegenerative diseases, including Alzheimer's disease. In this study, GE and its pure components, gastrodin and 4-hydroxybenzyl alcohol (4HBA), were applied to β-amyloid-induced BV2 mouse microglial cells. Materials and Methods Cell viability was assessed by the MTT assay and Western blotting was also performed. Results: β-amyloid-induced cell death was shown to be induced time- and dose-dependently. To examine the cell death mechanism, we confirmed the involvement of ER stress signaling. C/EBP homologous protein (CHOP), a pro-apoptotic ER stress protein, was expressed at high levels but glucose-regulated protein 78 (GRP78), an anti-apoptotic ER stress protein with chaperone activity, was only slightly affected by treatment with β-amyloid. However, pretreatment with GE and its components inhibited the expression of CHOP but increased that of GRP78 in β-amyloid-treated cells. This study also showed that a single treatment with GE extracts, gastrodin, or 4HBA induced the expression of GRP78, a marker for enhanced protein folding machinery, suggesting a protective mechanism for GE against β-amyloid. Conclusions: This study reveals the protective effects of GE against β-amyloid-induced cell death, possibly through the enhancement of protein folding machinery of a representative protein, GRP78, and the regulation of CHOP in BV2 mouse microglial cells.
Descritores: Amiloide/farmacologia
Álcoois Benzílicos/farmacologia
Morte Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Microglia/efeitos dos fármacos
-Álcoois Benzílicos/isolamento & purificação
Glucosídeos/isolamento & purificação
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central

  3 / 3 LILACS  
              first record previous record
para imprimir
Id: lil-90207
Autor: Silva, Herman; Landa, Agustín; Agosín, Eduardo.
Título: Aislamiento, selección y caracterización de hongos ligninolíticos chilenos / Isolation, selection and characterization of chilean ligninolytic fungi
Fonte: Arch. biol. med. exp;23(1):41-9, mayo 1990. tab.
Idioma: es.
Projeto: Universidad de Chile. Fondo de Desarrollo; . FONDECYT; . PNUD. Biodegradación y Aprovechamiento de Recursos lignocelulósicos.
Descritores: Fungos/enzimologia
-Álcoois Benzílicos/metabolismo
Biodegradação Ambiental
Fungos/isolamento & purificação
Monofenol Mono-Oxigenase/metabolismo
Responsável: CL1.1 - Biblioteca Central

página 1 de 1

Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  

Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde