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Id: biblio-1370034
Autor: Paggiaro, André Oliveira; Mathor, Mônica Beatriz; Carvalho, Viviane Fernandes de; Pólo, Eugenio; Herson, Marisa Roma; Ferreira, Marcus Castro.
Título: Estabelecimento de protocolo de glicerolização de membranas amnióticas para uso como curativo biológico / Establishment of amniotic membranes glycerolization protocol for use as biological dressing
Fonte: Rev. bras. queimaduras;9(1):2-6, Jan-Mar 2010.
Idioma: pt.
Resumo: Introdução: Pesquisadores têm procurado explorar várias alternativas terapêuticas, biológicas ou sintéticas, capazes de assegurar condições ideais ao leito da ferida, que favoreçam o processo de cicatrização. Uma opção menos onerosa é o uso de membranas amnióticas. Os curativos constituídos de âmnion formam uma barreira protetora contra as bactérias ambientais, aceleram a reepitelização das lesões e diminuem a dor local. Objetivo: O objetivo deste trabalho foi estabelecer protocolo de processamento de membranas amnióticas em altas concentrações de glicerol. Método: Foram obtidas 3 amostras de membranas amnióticas, que preenchiam os critérios de inclusão e que as gestantes concordaram em ceder o material para pesquisa. Resultados: Os exames de cultura do material no momento da captação mostravam ausência de crescimento bacteriano ou de fungos. As sorologias das pacientes eram todas negativas. Conclusão: Nesse trabalho, buscamos estabelecer um protocolo de conservação de membranas amnióticas baseado na glicerolização, pois se trata de um método de baixo custo, relativamente simples e de fácil estocagem do material. Apresenta como desvantagem a sua alta toxicidade celular, resultando em destruição das células do tecido, porém preserva a integridade estrutural tecidual, conforme demonstrado em nossos resultados macroscópicos e microscópicos.

Background: Researchers have attempted to explore various alternative therapies, biological or synthetic, capable of providing ideal conditions to the wound bed to promote the healing process. An option less costly is the use of amniotic membranes. Dressings consist of amnion forms a protective barrier against environmental bacteria, accelerate reepithelialization of lesions and reduce local pain. Objective: The aim of this study was to establish protocol processing of membranes in high concentrations of glycerol. Methods: Three samples were obtained from amniotic membranes who met the inclusion criteria and that the pregnant agreed to donate the material for research. Results: The examinations of material culture at the time of capture showed no bacterial or fungal growth. The serology of the patients was all negative. Conclusion: In this paper, we established a protocol for the conservation of membranes based on glycerol because it is a low-cost, relatively simple and easy storage of the material. Presents the disadvantage of its high cell toxicity, resulting in destruction of tissue cells, but preserves the structural integrity of tissue as shown in our results the microscopic and macroscopic.
Descritores: Curativos Biológicos/provisão & distribuição
Queimaduras/terapia
-Projetos de Pesquisa
Guias como Assunto/normas
Glicerol/administração & dosagem
Âmnio
Responsável: BR1.1 - BIREME


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Id: biblio-1349871
Autor: Javadov, Mirkhalig; Karatay, Emrah; Cumbul, Alev; Orman, Suleyman; Aysan, Erhan.
Título: Effects of glycerol and sodium pentaborate containing new formulation on sleeve gastrectomy model in rats
Fonte: Acta cir. bras;36(11):e361105, 2021. tab, graf.
Idioma: en.
Resumo: ABSTRACT Purpose: The development of cutting surface leakage and postoperative peritoneal adhesions (PPA) after sleeve gastrectomy (SG) are the most serious operative complications. We investigated the effectiveness of the newly developed glycerol and sodium pentaborate containing formulation on the prevention of these complications. Methods: Sixteen Sprague Dawley rats (mean weight 310 ± 50 g, mean age 3 months old) were divided into two groups, consisting of eight rats in each. SG and a double-layer suture technique were performed for each group. In study group, there was the mixture of 2 mL 3% glycerol plus 3% sodium pentaborate formulation, and in the control group 2 mL 0.9% NaCl was injected into the peritoneal cavity. Rats were sacrificed after 30 days, then macroscopic adhesion grade scoring and histopathological evaluations were assessed. Results: Macroscopic PPA scores in the control and study groups were 2.75 ± 0.16 and 1.50 ± 0.327, respectively (p = 0.004). Histopatologic fibrosis scores in the control and study groups were 0.87 ± 0.125 and 2.00 ± 0.26, respectively (p = 0.002). Conclusions: In SG operation model, glycerol plus sodium pentaborate compound decreased PPA formation and also increased stomach cut surface line fibrosis. This new formulation is hopeful for more safe SG operations.
Descritores: Gastrectomia
Glicerol
-Boratos
Aderências Teciduais/prevenção & controle
Ratos Sprague-Dawley
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


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Id: lil-670133
Autor: Bernal, Mauricio; Tinoco, Lizeth K; Torres, Luz; Malagón-Romero, Dionisio; Montoya, Dolly.
Título: Evaluating Colombian Clostridium spp. Strains hydrogen production using glycerol as substrate
Fonte: Electron. j. biotechnol;16(2):5-5, Mar. 2013. ilus, tab.
Idioma: en.
Resumo: Background: The ability for hydrogen production of 13 native strains of Clostridium spp. strains isolated from Colombian soil was evaluated using glycerol substrate. Glycerol to hydrogen conversion was investigated using a batch fermentation reactor and industrial glycerol source (50 g.l-1, pH 7.00). Results: The results were quantified regarding acids, hydrogen, biomass and solvent production. The selected strain gave good hydrogen over production output at 14.4 mmol H2.l-1, productivity 0.3167 mg H2.h-1 l-1 culture mediumand yield 0.1962 mol H2.mol-1 glycerol. A further fermentation assay a 4.0 liter batch reactor let to being 0.26 mg.l-1.h-1 after 18 hrs of fermentation. Logistic model, Luedeking-Piret model and Luedeking-Piret modified models were used for modeling changes in cell growth, hydrogen production and substrate consumption (Correlation coefficients R² = 0.95 for biomass substrate, R² = 0.77 hydrogen production). Conclusions: Our results indicate that hydrogen production through glycerol bioconversion by native strains is possible.
Descritores: Clostridium/metabolismo
Hidrogênio/metabolismo
-Biodegradação Ambiental
Biomassa
Reatores Biológicos
Fermentação
Técnicas de Cultura Celular por Lotes
Glicerol/metabolismo
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1370755
Autor: Silva, Jean Breno Silveira da; Lino, Leticia Araujo; Andrade, Claudia Roberta de.
Título: Processamento e conservação de enxertos de pele: uma abordagem com foco nos métodos de criopreservação, liofilização e glicerolização / Skin grafts processing and conservation: an approach focusing on cryopreservation, freeze-drying and glycerol conservation methods
Fonte: Rev. Ciênc. Méd. Biol. (Impr.) = J. med. biol. sci;21(1):117-122, maio 05,2022. fig.
Idioma: pt.
Resumo: Introdução: o uso de substitutos cutâneos para o tratamento de diversas feridas graves é uma forma eficiente de prevenir infecções e favorecer o processo de reepitelização. No entanto, tecidos biológicos estão suscetíveis a degradação e contaminação. Por isso, devem ser submetidos a rigorosos protocolos de processamento e testes que comprovem suas contribuições benéficas e segurança de aplicação. Objetivo: trazer uma abordagem sobre as principais características dos métodos de criopreservação, glicerolização e liofilização e sua consequencia nos aspectos imunológicos, microbiológicos e de viabilidade tecidual de enxertos de pele humana. Metodologia: foi realizada uma busca online utilizando as palavras chaves "criopreservação", "liofilização", "glicerolização", "enxertos", "processamento tecidual" e "engenharia dos tecidos" em múltiplas combinações nos bancos de dados PubMed, LILACS e ScienceDirect. Resultados: 200 artigos científicos foram obtidos, 26 excluídos por duplicidade, 92 selecionados para leitura integral a partir da leitura de seus resumos e 27 utilizados na construção desta revisão. A liofilização e a glicerolização são métodos semelhantes considerando a viabilidade tecidual. O uso de glicerol traz como principal desvantagem sua citotoxicidade quando comparado aos outros métodos. A criopreservação mantém os tecidos viáveis. Contudo, pode ser mais cara e trazer riscos de transmissão de microorganismos patogênicos. De modo geral, não é bem estabelecido quais os melhores métodos de conservação para uma adequada conservação da viabilidade dos enxertos de pele. Considerações Finais: os 3 métodos, liofilização, glicerolização e criopreservação, possuem aplicabilidade na conservação de enxertos. A falta de padronização na aplicação de enxertos apesar de sua frequente aplicação e a escassez de estudos recentes sobre o tema justificam o presente estudo.

Introduction: the use of skin substitutes for treatment of several wounds is an efficient way to prevent infections and allow the re-epithelialization process. However, biological tissues are susceptible to degradation and contamination. Therefore, they must undergo rigorous processing and testing protocols that prove their beneficial contributions and application security. Objective:to bring an approach on the main characteristics of cryopreservation, freeze-drying and glycerol conservation methods and their implications on immunological, microbiological and tissue viability aspects when applied to human skin grafts. Methodology:a mostly online search was performed using the keywords "cryopreservation", "freeze-drying", "glycerol conservation", "grafts", "tissue processing" and "tissue engineering" in multiple combinations in PubMed, LILACS and ScienceDirect databases. Results: 200 scientific articles were rescued, 26 excluded by duplicity, 92 selected for full reading from the reading of their abstracts and 27 used in the construction of this review. Freeze-drying and glycerol conservation are similar methods, with glycerol conservation having greater economic advantage. The use of glycerol presents cytotoxicity when compared to the other methods. Cryopreservation keeps tissues viable, however, is more expensive and carry risks of transmission of pathogenic microorganisms. Overall, there is a lack of clarity about the importance of viability in the performance of skin grafts. Final considerations: the 3 methods have applicability in graft conservation. The lack of standardization in graft application despite its frequent application and the scarcity of recent studies on the subject justify the present study.
Descritores: Ferimentos e Lesões
Criopreservação
Aloenxertos
Liofilização
Glicerol
-Revisão
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Revisão
Responsável: BR342.1 - Biblioteca Universitária de Saúde


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Id: lil-714275
Autor: Szymanowska-Powalowska, Daria; Leja, Katarzyna.
Título: An increasing of the efficiency of microbiological synthesis of 1, 3-propanediol from crude glycerol by the concentration of biomass
Fonte: Electron. j. biotechnol;17(2):72-78, Mar. 2014. ilus, graf, tab.
Idioma: en.
Projeto: European Union. European Regional Development Fund. Innovative Economy Operational Programme 2007-2013.
Resumo: Background 1,3-Propanodiol (1,3-PD), is used in the production of polytrimethylene terephthalate (PTT), an aromatic polyester that exhibits high elastic recoveries. It is also employed as a supplement with low solidification properties, a solvent and a lubricant in the formof propylene glycol. 1,3-PD is effectively synthesized by a microbiological way from crude glycerol. The main problem of this technology is using a high concentration of glycerol, which is a limiting factor for bacteria cells growth (especially in batch fermentation). Results In this work, the influence of different glycerol concentration in batch fermentation on Clostridium butyricum DSP1 metabolism was investigated. The biomass was concentrated for two times with the use of membrane module (in case of increasing kinetic parameters). Increased optical density of bacteria cells six times increased the productivity of 1,3-PD in cultivation with 20 g/L of glycerol at the beginning of the process, and more than two times in cultivation with 60-80 g/L. Also the possibility of complete attenuation of 140 g/L of crude glycerol in the batch fermentation was investigated. During the cultivation, changes of protein profiles were analyzed. The most significant changes were observed in the cultivation in the medium supplemented with 80 g/L of glycerol. They related mainly to the DNA protein reconstructive systems, protective proteins (HSP), and also the enzymatic catalysts connected with glycerol metabolic pathway. Conclusions The application of filtration module in batch fermentation of crude glycerol by C. butyricum DSP1 significantly increased the productivity of the process.
Descritores: Propilenoglicóis/síntese química
Clostridium butyricum
Glicerol/metabolismo
-Cinética
Biomassa
Meios de Cultura
Proteômica
Fermentação
Filtração/métodos
Proteínas de Choque Térmico
Responsável: CL1.1 - Biblioteca Central


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Id: lil-730265
Autor: Szymanowska-Powalowska, Daria.
Título: 1, 3-Propanediol production from crude glycerol by Clostridium butyricum DSP1 in repeated batch
Fonte: Electron. j. biotechnol;17(6):322-328, Nov. 2014. graf, tab.
Idioma: en.
Projeto: Operational Programme - Innovative Economy, 2007-2013, Priority 1; . Research and development of modern technologies Activity 1.1; . Support for scientific research for the establishment of a knowledge-based economy Subactivity 1.1.2; . Strategic programs of scientific research and development.
Resumo: Background The production of biofuels from renewable energy sources is one of the most important issues in industrial biotechnology today. The process is known to generate various by-products, for example crude glycerol, which is obtained in the making of biodiesel from rapeseed oil. Crude glycerol may be utilized in many ways, including microbial conversion to 1,3-propanediol (1,3-PD), a raw material for the synthesis of polyesters and polyurethanes. Results The paper presents results of a study on the synthesis of 1,3-propanediol from crude glycerol by a repeated batch method with the use of Clostridium butyricum DSP1. Three cycles of fermentation medium replacement were carried out. The final concentration of 1,3-PD was 62 g/L and the maximum productivity, obtained during the second cycle, reached 1.68 g/L/h. Additionally, experiments conducted in parallel to the above involved using the entire quantity of the culture broth removed from the bioreactor to inoculate successive portions of fermentation media containing crude glycerol at concentrations of 80 g/L and 100 g/L. Under those conditions, the maximum 1,3-PD concentrations were 43.2 g/L and 54.2 g/L. Conclusions The experiments proved that by using a portion of metabolically active biomass as inoculum for another fermentation formula it is possible to eliminate the stage of inoculum growth and thereby reduce the length of the whole operation. Additionally, that strategy avoids the phase of microbial adaptation to a different source of carbon such as crude glycerol, which is more difficult to utilize, thus improving the kinetic parameters of 1,3-PD production.
Descritores: Propilenoglicóis/metabolismo
Clostridium butyricum/metabolismo
Glicerol/metabolismo
-Estresse Fisiológico
Cromatografia Líquida de Alta Pressão
Reatores Biológicos
Meio Ambiente
Biocombustíveis
Fermentação
Técnicas de Cultura Celular por Lotes
Responsável: CL1.1 - Biblioteca Central


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Id: lil-745581
Autor: Szymanowska-Powałowska, Daria.
Título: The effect of high concentrations of glycerol on the growth, metabolism and adaptation capacity of Clostridium butyricum DSP1
Fonte: Electron. j. biotechnol;18(2):128-133, Mar. 2015. ilus, graf, tab.
Idioma: en.
Projeto: SORMISOL; . National Centre for Research and Development. Applied Research Programme. Development of an innovative production technology of 2nd generation bioethanol from biomass of sorghum (Sorghum sp.) and Miscanthus (Miscanthus sp.).
Resumo: Background The production of biofuels from renewable energy sources is one of the most important issues in biotechnology today. The process is known to generate various by-products, for example glycerol that is obtained in the making of biodiesel from rapeseed oil. Crude glycerol may be utilized in many ways, including microbial conversion to 1,3-propanediol. The main drawback of that technology is the use of high concentrations of glycerol, which inhibits the growth of bacterial cells. Results This study investigated the impact of crude glycerol on Clostridium butyricum DSP1 and its ability to adapt to an environment of high osmotic pressure. It was found that a crude glycerol concentration of up to 70 g/L did not have an inhibitory effect on C. butyricum DSP1. Adaptation procedures involving the passage of metabolically active biomass from a fermentation medium with a lower concentration of crude glycerol to one with a greater substrate concentration allowed breaking the barrier of high osmotic pressure (150 g/L crude glycerol) and receiving a 1,3-PD concentration of 74 g/L in a batch culture operation. The work looked into intracellular modifications shown by proteomic profiling in order to explain the mechanisms underlying the response and adaptation of bacterial cells exposed to unfavorable environmental conditions. Conclusions This study of the effect of glycerol on the growth and metabolism of C. butyricum DSP1 demonstrated that the maximum substrate concentrations that do not inhibit the metabolic activity of bacterial cells are 90 g/L and 70 g/L for pure and crude glycerol, respectively.
Descritores: Adaptação Fisiológica
Clostridium butyricum/crescimento & desenvolvimento
Clostridium butyricum/metabolismo
Glicerol/metabolismo
-Pressão Osmótica
Propilenoglicóis
Estresse Fisiológico
Proteínas/análise
Meio Ambiente
Biocombustíveis
Fermentação
Técnicas de Cultura Celular por Lotes
Glicerol/análise
Responsável: CL1.1 - Biblioteca Central


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Id: lil-764027
Autor: Szymanowska-Powalowska, Daria.
Título: Corrigendum to The effect of high concentrations of glycerol on the growth, metabolism and adaptation capacity of Clostridium butyricum DSP1
Fonte: Electron. j. biotechnol;18(5):387-387, Sept. 2015.
Idioma: en.
Descritores: Adaptação Biológica
Clostridium butyricum/crescimento & desenvolvimento
Clostridium butyricum/metabolismo
Glicerol/análise
Tipo de Publ: Errata
Responsável: CL1.1 - Biblioteca Central


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Id: lil-772286
Autor: Kot, Anna Maria; Blazejak, Stanislaw; Kurcz, Agnieszka; Gientka, Iwona.
Título: Biodegradation of deproteinized potato wastewater and glycerol during cultivation of Rhodotorula glutinis yeast
Fonte: Electron. j. biotechnol;18(6):428-432, Nov. 2015. graf, tab.
Idioma: en.
Resumo: Background Deproteinized potato wastewater and glycerol are two by-products which are difficult to dispose. The objective of this study was to determine the ability of Rhodotorula glutinis to use glycerol and nitrogen compounds present in deproteinized potato wastewater and to evaluate the ability of simultaneous biodegradation of potato wastewater and glycerol via microbiological methods. Results It has been found that R. glutinis used glycerol and potato wastewater as a source of carbon and nitrogen, respectively. The highest degree of glycerol content (70.6%) reduction was found after cultivation of the investigated strain using a medium with 5% glycerol. In this medium, a significant reduction in the total protein content, estimated at 61%, was observed. The process of 72 h cultivation of yeast in a medium containing potato wastewater and 5% glycerol reduced the chemical oxygen demand (COD) more than 77%. Supplementation of media with high doses of glycerol (i.e. 20 and 25%) led to decreased metabolic activity in the yeast strain tested. Conclusion It has been found that there is a possibility of simultaneous biodegradation of potato wastewater and glycerol during the cultivation of R. glutinis.
Descritores: Rhodotorula
Biodegradação Ambiental
Águas Residuárias
Glicerol
-Leveduras
Solanum tuberosum
Análise da Demanda Biológica de Oxigênio
Resíduos Industriais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-893652
Autor: Botelho, Maria Paula Jacobucci; Isolan, Cristina Pereira; Schwantz, Júlia Kaster; Lopes, Murilo Baena; Moraes, Rafael Ratto de.
Título: Rubbing time and bonding performance of one-step adhesives to primary enamel and dentin
Fonte: J. appl. oral sci;25(5):523-532, Sept.-Oct. 2017. tab, graf.
Idioma: en.
Resumo: Abstract Objectives: This study investigated whether increasing the concentration of acidic monomers in one-step adhesives would allow reducing their application time without interfering with the bonding ability to primary enamel and dentin. Material and methods: Experimental one-step self-etch adhesives were formulated with 5 wt% (AD5), 20 wt% (AD20), or 35 wt% (AD35) acidic monomer. The adhesives were applied using rubbing motion for 5, 10, or 20 s. Bond strengths to primary enamel and dentin were tested under shear stress. A commercial etch-and-rinse adhesive (Single Bond 2; 3M ESPE) served as reference. Scanning electron microscopy was used to observe the morphology of bonded interfaces. Data were analysed at p<0.05. Results: In enamel, AD35 had higher bond strength when rubbed for at least 10 s, while application for 5 s generated lower bond strength. In dentin, increased acidic monomer improved bonding only for 20 s rubbing time. The etch-and-rinse adhesive yielded higher bond strength to enamel and similar bonding to dentin as compared with the self-etch adhesives. The adhesive layer was thicker and more irregular for the etch-and-rinse material, with no appreciable differences among the self-etch systems. Conclusion: Overall, increasing the acidic monomer concentration only led to an increase in bond strength to enamel when the rubbing time was at least 10 s. In dentin, despite the increase in bond strength with longer rubbing times, the results favoured the experimental adhesives compared to the conventional adhesive. Reduced rubbing time of self-etch adhesives should be avoided in the clinical setup.
Descritores: Colagem Dentária/métodos
Adesivos Dentinários/química
Bis-Fenol A-Glicidil Metacrilato/química
Esmalte Dentário/efeitos dos fármacos
Dentina/efeitos dos fármacos
Metacrilatos/química
-Solventes/química
Propriedades de Superfície/efeitos dos fármacos
Fatores de Tempo
Teste de Materiais
Água/química
Microscopia Eletrônica de Varredura
Reprodutibilidade dos Testes
Análise de Variância
Falha de Restauração Dentária
Resistência ao Cisalhamento
Glicerol/química
Limites: Humanos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME



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