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Id: biblio-969670
Autor: Costa, Taiza Florencio; Felli, Vanda Elisa Andres; Sanchez, Maritza Consuelo Ortiz; Ferreira, Simone Cruz Machado; Silvino, Zenith Rosa; Souza, Deise Ferreira de.
Título: Gerenciamento intra-hospitalar dos resíduos químicos perigosos manuseados pela enfermagem / Intra-hospital management of hazardous chemical waste handled by nurses / Gestión intrahospitalaria de los residuos químicos peligrosos manejados por la enfermería
Fonte: Rev. enferm. UERJ;26:e19376, jan.-dez. 2018. tab.
Idioma: pt.
Resumo: Objetivo: avaliar as etapas de gerenciamento dos resíduos químicos perigosos - formaldeído e ortoftaldeído, manuseados pela enfermagem. Método: pesquisa descritiva e coleta de dados realizada de setembro a dezembro de 2012, no Hospital Universitário da Universidade de São Paulo, por meio da aplicação da Ficha de Informação de Gerenciamento de Resíduo Químico Perigoso dos resíduos formaldeído e ortoftaldeído gerados, respectivamente, no centro obstétrico e endoscopia. Projeto aprovado por Comitê de Ética em Pesquisa. Resultados: a análise estatística dos dados evidenciou que foram atendidas 3 (13%) especificações do total de 23 (100%) necessárias, com prevalência da identificação, seguida da segregação e nenhuma do transporte interno dos resíduos. Há necessidade de a instituição cumprir as especificações de identificações gerais em relação ao número total de avaliações nos recipientes de resíduos de formaldeído e ortoftaldeído. Conclusão: esta pesquisa proporcionou importantes diretrizes para a elaboração do Plano de Gerenciamento de Resíduos Químicos Perigosos da atenção hospitalar.

Objective: to evaluate the stages in management of hazardous chemical waste handled by nursing staff. Method: in this descriptive study, data was collected from September to December 2012 at São Paulo University Hospital, by applying the Hazardous Chemical Waste Management Information Sheet for formaldehyde and ortho-phthalaldehyde waste in the obstetric center and endoscopy, respectively. The study was approved by the research ethics committee. Results: data analysis showed that 3 (13%) of the 23 (100%) required specifications were met, predominantly identification, followed by segregation, and that none on internal waste transportation were met. The institution needs to comply with general identification specifications regarding the total number of evaluations of formaldehyde and orthophthaldehyde waste containers. Conclusion: this study also yielded important guidelines for preparation of the hospital care Hazardous Chemical Waste Management Plan.

Objetivo: evaluar las etapas de la gestión de los residuos químicos peligrosos - formaldehído y ortoftaldehído, manejados por la enfermería. Métodos: investigación descriptiva y recolección de datos realizada, de septiembre a diciembre de 2012, en el Hospital Universitario de la Universidad de São Paulo, por medio de la aplicación de la Ficha de Información de Gestión de Residuo Químico Peligroso de los residuos de formaldehído y ortoftaldehído generados, respectivamente, en el Centro de Obstetricia y Endoscopía. Proyecto aprobado por el Comité de Ética en Investigación. Resultados: el análisis estadístico de los datos mostró que fueron atendidas 3 (13%) especificaciones del total de 23 (100%) requeridas, con prevalencia de la identificación, seguida por la segregación y ninguna relacionada al transporte interno de los residuos. Es necesario que la institución cumpla con las especificaciones de identificaciones generales en relación al número total de evaluaciones en los recipientes de residuos de formaldehído y ortoftaldehído. Conclusión: la investigación proporcionó importantes directrices para la elaboración del Plan de Gestión de Residuos Químicos Peligrosos de la atención hospitalaria.
Descritores: Resíduos Perigosos
Saúde do Trabalhador
Gerenciamento de Resíduos
Resíduos Químicos
Enfermagem do Trabalho
-Brasil
Saúde Ambiental
Epidemiologia Descritiva
Formaldeído
Responsável: BR1366.1 - Biblioteca Biomédica B - CB/B (Odontologia e Enfermagem)


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Id: biblio-1134424
Autor: Sajadi, Ensieh; Raoofi, Amir; Abdi, Shabnam; Azimi, Hadi; Abdollahifar, Mohammad-Amin.
Título: The modified method of luxol fast blue for paraffin-embedded myelin sheath staining / El método modificado de luxol fast blue para la tinción de mielina fijada en parafina
Fonte: Int. j. morphol;38(5):1197-1200, oct. 2020. graf.
Idioma: en.
Projeto: Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Resumo: SUMMARY: Histological techniques are the study of animal and human tissues through staining and examining them under a microscope. To demonstrate the axonal degeneration and demyelination in histological studies, the Luxol Fast Blue staining is gold standard techniques. In this study, a new histochemical method based on modified Luxol Fast Blue for the staining of the myelin sheath in sciatic nerve tissues described. The sciatic nerves of rats were removed and then the sciatic nerve was immersed in 10 % formaldehyde for one week and embedded in paraffin block. Next, thin sections (5 µm) were cut, using a microtome and stained with conventional and modified Luxol Fast Blue. Our results showed that a new method of modified Luxol Fast Blue staining can accurately identify the myelin in the sciatic nerve fibers. The current study showed that the Luxol Fast Blue combination with Light Green has a good effect on myelin coloration, and the results of this study are comparable to LFB combination with Sirius red.

RESUMEN: Las técnicas histológicas son el estudio de tejidos animales y humanos mediante tinción y examen bajo un microscopio. Para demostrar la degeneración axonal y la desmielinización en estudios histológicos, la tinción Luxol Fast Blue es una técnica estándar de oro. En este estudio, se describe un nuevo método histoquímico basado en Luxol Fast Blue modificado para la tinción de mielina en los tejidos del nervio ciático. Se seccionaron los nervios ciáticos de ratas y luego el nervio ciático se sumergió en formaldehído al 10 % durante una semana y se fijó en bloque de parafina. Posteriormente, se cortaron secciones delgadas (5 µm) usando un microtomo y se tiñeron con Luxol Fast Blue convencional y modificado. Nuestros resultados mostraron que un nuevo método de tinción Luxol Fast Blue modificado puede identificar con precisión la mielina en las fibras del nervio ciático. El estudio actual mostró que la combinación Luxol Fast Blue con Light Green es un buen efecto sobre la coloración de mielina, y los resultados de este estudio son comparables a la combinación LFB con Sirius red.
Descritores: Nervo Isquiático/anatomia & histologia
Coloração e Rotulagem/métodos
Bainha de Mielina
-Parafina
Técnicas Histológicas
Formaldeído
Microscopia/métodos
Limites: Animais
Ratos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1134450
Autor: Haque, Ziaul; Rahman, Asabur; Khan, Zahirul Islam; Hussan, Mohammad Tufazzal; Alam, Mahmudul.
Título: Alcohol-based fixatives can better preserve tissue morphology than formalin / Fijadores a base de alcohol pueden preservar mejor la morfología tisular que la formalina
Fonte: Int. j. morphol;38(5):1371-1375, oct. 2020. tab, graf.
Idioma: en.
Resumo: SUMMARY: Fixation is a crucial step in processing of tissue specimen for preservation of cellular architecture and composition of cells. Alcohol-based fixatives are considered some of the most promising alternatives to formalin. We evaluated the performance of alcohol-based fixatives (EthMeth and methacarn) and formalin as a comparator fixative in the research laboratory. Following 24 hours of fixation, tissue morphology and cellular details of the liver, spleen and brain (cerebral cortex) were evaluated. Morphological characteristics were evaluated by gross observations and analyzing cellular details, tissue architecture and overall staining characteristics (Hematoxylin and Eosin). EthMeth and methacarn fixation gave generally comparable and satisfactory results on the tissue morphology and subsequent identification of tissue characteristics. Particularly, tissues were well preserved and all nuclear as well as cytoplasmic details were clearly visible. However, formalin fixed tissues showed some peculiarity such as improper fixation, mild shrinkage, and alterations of tissue components. These results confirm that alcohol-based fixation is the superior alternative to formalin for preservation of tissue morphology. However, it is required to standardize the formalin-free methods and harmonize diagnosis in the laboratory worldwide.

RESUMEN: La fijación es un paso crucial en el procesamiento de muestras de tejido para preservar la arquitectura celular y la composición de las células. Los fijadores a base de alcohol se consideran algunas de las alternativas más prometedoras a la formalina. Evaluamos el rendimiento de los fijadores a base de alcohol (EthMeth y metacarn) y formalina como fijador comparativo en el laboratorio de investigación. Después de 24 horas de fijación, se observó la morfología del tejido y los detalles celulares del hígado, bazo y corteza cerebral. Se evaluaron las características morfológicas mediante observaciones generales y analizando detalles celulares, arquitectura de tejidos y características generales de tinción (hematoxilina y eosina). La fijación de EthMeth y metacarn dio resultados generalmente comparables y satisfactorios en la morfología del tejido y la posterior identificación de las características del mismo. Particularmente, los tejidos estaban bien conservados y todos los detalles nucleares y citoplasmáticos eran claramente visibles. Sin embargo, los tejidos fijados con formalina mostraron cierta peculiaridad, tal como una fijación inadecuada, la contracción leve y alteraciones de los componentes del tejido. Estos resultados confirman que la fijación a base de alcohol es la mejor alternativa a la formalina, para preservar la morfología del tejido. Sin embargo, es necesario estandarizar los métodos sin formalina y armonizar el diagnóstico en los laboratorios.
Descritores: Fixação de Tecidos/métodos
Álcoois/química
Fixadores
Formaldeído/química
-Clorofórmio/química
Ácido Acético/química
Metanol/química
Tipo de Publ: Estudo Comparativo
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1178915
Autor: Segurondo Loza, Romina; Pantoja, María Rosa; Rocha, Eliana.
Título: Determinación de la genuinidad en jugos de naranja comercializados en los supermercados de la ciudad de La Paz / Determination of the genuineness in juices of orange marketed in supermarkets from the city of La Paz
Fonte: Con-ciencia (La Paz);1(1):105-112, oct. 2013. ilus..
Idioma: es.
Resumo: La producción mundial de jugos cítricos concentrados se estima en 2,6 millones de toneladas. El jugo de naranja representa el 88% del total. El mercado es liderizado por Brasil con el 50% del volumen elaborado, seguido por EEUU con el 40%. En Bolivia el mercado de frutas se encuentra en continuo crecimiento, tanto para importación como para consumo interno. Actualmente se comercializan bebidas a base de jugo de frutas tanto de importación como de fabricación nacional, las mismas que son sometidas a control por los Organismos Oficiales Ministerio de Salud y Deportes, y el Servicio Nacional de Sanidad Agropecuaria e Inocuidad Alimentaria - SENASAG bajo la Normativa Nacional e Internacional reconocida como es la Norma Boliviana y el Codex Alimentarius, al igual que todos los productos alimenticios para garantizar su calidad e inocuidad. El presente trabajo pretende determinar la genuinidad de los jugos comercializados en nuestro medio para lo cual el método por espectrofotometría fue el que nos permitió cuantificar la concentración de prolina como aminoácido predominante presente en la naranja. Actualmente el análisis laboratorial se basa principalmente en las determinaciones de parámetros fisicoquímicos que necesitan ser complementados con otros métodos analíticos como: la Cuantificación de Prolina por Espectrofotometría y el Indice de Formol propuestos en éste trabajo, capaces de determinar la genuinidad de jugos de naranja presentes en el comercio de la ciudad de La Paz, y así colaborar con los Organismos de Control para cumplir a cabalidad con la responsabilidad de garantizar que los productos consumidos por nuestra población cumplen con lo declarado en sus etiquetas. El estudio reveló que todas las muestras analizadas cumplen con lo establecido en los parámetros fisicoquímicos, mientras que en los parámetros de genuinidad sugeridos en el presente trabajo, el 62% cumple con los límites de referencia del índice de formol y sólo un 44% con los límites de prolina.

World production of citrus juice concentrates, is estimated at 2.6 million tonnes. Orange juice accounts for 88% of the total. The market is led by Brazil with 50% of the volume produced, followed by U.S. with 40%. In Bolivia the fruit market is still growing, both for imports and for domestic consumption Currently marketed drinks based on fruit juice both import and domestic manufacture, they are subject to control by government agencies Ministry of Health and Sports and the National Service of Agricultural Health and Food Safety - SENASAG under national and international standards is recognized as the Standard Bolivian and the Codex Alimentarius, like all food products to ensure quality and safety. This paper aims to determine the genuine from the juices marketed in our area for which the method was spectrophotometrically which allowed us to quantify the concentration of the amino acid praline as a dominant presence in the orange Currently, the laboratory analysis is mainly based on the determinations of physical and chemical parameters that need to be complemented by other analytical methods such as: Quantifying Proline by spectrophotometry, and Formol Index proposed in his work, able to determine the genuine orange juice in the trade of the city of La Paz, and collaborate with the supervisory bodies to fulfill the responsibility to ensure that the products consumed by our population satisfied with what was stated on their labels. The study revealed that all the samples tested complied with the parameters established physicochemical while in the parameters of genuine suggested in the present study, 62.5% comply with the limits of the reference rate of formol and only 43, 75% with the limits of proline.
Descritores: Espectrofotometria
Prolina
-Gestão da Qualidade
Sucos
Parâmetros de Referência
Sucos de Frutas e Vegetais
Formaldeído
Aminoácidos
Tipo de Publ: Artigo Clássico
Responsável: BO138.1 - Biblioteca Central


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Id: biblio-1143732
Autor: Villadiego-Molinares, Marisol Margarita; Ramírez-Martínez, Johanna Alejandra; Rodriguez-Pulido, Alba Isabel.
Título: Formaldehyde in occupational environments: literature review and an occupational health surveillance proposal / Formaldehído en ambientes laborales: revisión de la literatura y propuesta de vigilancia ocupacional
Fonte: Rev. Fac. Med. (Bogotá);68(3):425-437, July-Sept. 2020. tab, graf.
Idioma: en.
Resumo: Abstract Introduction: Formaldehyde is a substance widely used in the industry; however, it is classified as mutagenic and carcinogenic to humans. In order to determine the risk of workers who are occupationally exposed to formaldehyde, it is necessary to monitor its environmental concentration levels and the biomarkers that allow identifying its potential health effects. Unfortunately, in Colombia there are not guidelines on occupational exposure to this substance. Objective: To review recent studies on occupational exposure to formaldehyde to design a monitoring and surveillance strategy for Colombian workers exposed to this substance. Materials and methods: A literature review was conducted in PubMed, MedLine, Science-Direct and Embase using the following search strategy: articles on occupational exposure to formaldehyde published in English or Spanish between 2013 and 2017. The following search terms were used: "occupational exposure", "formaldehyde" "mutagenicity test" y "DNA adducts" and their Spanish equivalents. Results: The initial search yielded 103 articles, of which only 36 met the inclusion criteria. Conclusions: Proper management of the risk derived from occupational exposure to formaldehyde, as well as the appropriate medical follow-up of these workers, requires the implementation of a series of interdisciplinary actions that allow the creation of a comprehensive occupational health surveillance system for workers exposed to this substance.

Resumen Introducción. El formaldehido es una sustancia ampliamente usada a nivel industrial; sin embargo, es considerada un agente mutagénico y carcinógeno para los humanos. Para determinar el grado de riesgo de los trabajadores ocupacionalmente expuestos (TOE) al formaldehido, debe hacerse un seguimiento de sus niveles de concentración ambiental y de los biomarcadores que permiten identificar su daño potencial para la salud. En Colombia, lamentablemente, no existen lineamientos respecto a la exposición ocupacional a esta sustancia. Objetivo. Revisar estudios recientes sobre exposición ocupacional a formaldehido para diseñar una estrategia de seguimiento y vigilancia de los TOE a esta sustancia en Colombia. Materiales y métodos. Se realizó una revisión de la literatura en PubMed, MedLine, ScienceDirect y Embase mediante la siguiente estrategia de búsqueda: artículos sobre exposición ocupacional a formaldehido publicados en inglés o español entre 2013 y 2017. Los términos de búsqueda fueron "occupational exposure", "formaldehyde" "mutagenicity test" y "DNA adducts" y sus equivalentes en español. Resultados. La búsqueda inicial arrojó 103 registros, sin embargo solo 36 artículos cumplieron los criterios de inclusión establecidos. Conclusiones. La gestión adecuada del riesgo derivado de la exposición ocupacional a formaldehido, asi como el seguimiento médico apropiado de estos trabajadores, requiere la implementación de una serie de acciones interdisciplinarias que permitan la creación de un sistema de vigilancia ocupacional integral de los TOE a esta sustancia.
Descritores: Exposição Ocupacional
-Biomarcadores
Formaldeído
Testes de Mutagenicidade
Responsável: CO136.2 - Biblioteca


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Id: biblio-1087710
Autor: Guerrero, Karlo; Arancibia, Alejandra; Caceres, Manuel; Aroca, German.
Título: Release of formaldehyde during the biofiltration of methanol vapors in a peat biofilter inoculated with Pichia pastoris GS115
Fonte: Electron. j. biotechnol;44:58-59, Mar. 2020. ilus.
Idioma: en.
Resumo: BACKGROUND: Methanol can be effectively removed from air by biofiltration (Shareefdeen et al., 1993; Babbitt et al., 2009 [1,2]). However, formaldehyde is one of the first metabolic intermediates in the consumption of methanol in methylotrophic microorganisms (Negruta et al., 2010 [3]), and it can be released out of the cell constituting a secondary emission. RESULTS: The total removal of methanol was achieved up to input loads of 263 g m−3 h−1 and the maximum elimination capacity of the system was obtained at an empty bed residence times of 90 s and reached 330 g m−3 h−1 at an input methanol load of 414 g m−3 h−1 and 80% of removal efficiency. Formaldehyde was detected inside the biofilter when the input methanol load was above 212 g m−3 h−1 . Biomass in the filter bed was able to degrade the formaldehyde generated, but with the increase of the methanol input load, the unconsumed formaldehyde was released outside the biofilter. The maximum concentration registered at the output of the system was 3.98 g m−3 when the methanol load was 672 g m−3 h−1 in an empty bed residence times of 60 s. CONCLUSIONS: Formaldehyde is produced inside a biofilter when methanol is treated in a biofiltration system inoculated with Pichia pastoris. Biomass present in the reactor is capable of degrading the formaldehyde generated as the concentration of methanol decreases. However, high methanol loads can lead to the generation and release of formaldehyde into the environment.
Descritores: Pichia/química
Metanol/química
Formaldeído/análise
-Volatilização
Filtros Biológicos
Biomassa
Reatores Biológicos
Meio Ambiente
Responsável: CL1.1 - Biblioteca Central


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Id: lil-761219
Autor: Abreu, VM; Azevedo, MGB; Falcão, JSA.
Título: Cosmetovigilância em alisantes capilares: Determinação do teor de formaldeído por espectrofotometria e avaliação do rótulo / Cosmetovigilance in hair straighteners: Determination of formaldehyde content by spectrophotometry and label evaluation
Fonte: Rev. ciênc. farm. básica apl;36(1), mar. 2015. tab, graf.
Idioma: pt.
Resumo: O objetivo do trabalho foi identificar e quantificar o formaldeído presente em formulações comerciais de alisamento capilar, sendo abordada a aplicação da cosmetovigilância a partir de ensaios organolépticos/ físico-químicos e análise dos rótulos. Oito formulações comerciais de marcas distintas foram submetidas à análise por espectrofotometria, baseado na reação entre formaldeído e ácido cromotrópico na presença de sulfato de magnésio, produzindo um complexo que permite identificar e quantificar a presença da substância ativa. As amostras A1, A3, A5 e A8 apresentaram uma concentração de formaldeído variando de 1,5 a 3,83% (p/v), o que corresponde a concentrações 7,5; 16,45; 7,9 e 19,15 vezes acima do permitido pela ANVISA, apresentando odor forte característico dessa substância ativa. Destas amostras, A3 e A5 não indicaram a presença de formaldeído no rótulo, além de ignorarem as informações de advertência e restrições de uso. Foi verificado ainda, a ausência do número de registro concedido pela ANVISA para a amostra A5, o que pode ser indício de produto clandestino. Quanto às características organolépticas e físico-químicas somente a amostra A2 apresentou resultados de viscosidade e centrifugação diferentes das demais. Diante desses resultados, conclui-se que 50% dos produtos analisados foram reprovados devido à presença de formaldeído fora da concentração permitida, ficando evidente a importância da implantação do sistema de cosmetovigilância para garantir a qualidade final dos produtos cosméticos, tendo em vista principalmente, a segurança e eficácia desses produtos.(AU)

This study aimed to identify and quantify the formaldehyde present in commercial formulations of hair straightening, being addressed the application of cosmetovigilance from organoleptic/physical- chemical tests and analysis of labels. Eight commercial formulations of different brands were analyzed by spectrophotometry, based on the reaction between formaldehyde and chromotropic acid in the presence of magnesium sulfate, producing a complex that allows to identify and quantify the presence of the active substance. The samples A1, A3, A5 and A8 presented a formaldehyde concentration ranging from 1.5 to 3.83% (w / v), which corresponds to concentrations 7.5, 16.45, 7.9 and 19.15 times higher than allowed by ANVISA, presenting strong odor characteristic of this active substance. These samples, A3 and A5 did not indicate the presence of formaldehyde on the label, in addition to ignoring the warning information and restrictions. Was further verified, the absence of registration number issued by ANVISA for sample A5, which may indicate clandestine product. Concerning the organoleptic and physico-chemical characteristics only sample A2 presented results of different viscosity and centrifugation of the others. Given these results, it is concluded that 50% of the analyzed products were failed, due to the presence of formaldehyde outside the permitted concentration, evidencing the importance of implementation the system cosmetovigilance to ensure the final quality of cosmetic products, in view of primarily, the safety and efficacy of these products.(AU)
Descritores: Rotulagem de Cosméticos
Formaldeído
Cabelo
Preparações para Cabelo/análise
-Espectrofotometria/métodos
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: biblio-1128576
Autor: Silvestre, Amanda Letícia Polli; Fagá, Maira; Almeida-Cincotto, Maria Gabriela José; Chiari-Andréo, Bruna Galdorfini; Formariz, Thalita Pedroni.
Título: Qualitative and quantitative analysis of formaldehyde in samples of hair straighteners and suitability of the labels
Fonte: Rev. ciênc. farm. básica apl;41:[10], 01/01/2020. tab, ilus.
Idioma: en.
Resumo: Formaldehyde is an active compound, irregularly used in hair products, that has the property of straighten and waterproofing the wires. However, it is highly toxic and can stimulate dermatological hypersensitivity and cancer. In this context it is of fundamental importance the inspection of these products that can be used in safe conditions for the consumer, without formaldehyde in concentrations higher than the allowed. Thus, the aim of this research was the qualitative and quantitative identification formaldehyde in samples of hair straighteners that was obtained by donation in the beauty salons of Araraquara-SP. In addition, the analysis of the packaging labels of the products tested were conducted, following the requirements of the national legislation - RDC 07/2015 which defines the mandatory labeling standards for cosmetic products. A qualitative analysis for formaldehyde identification is based on the formation of a purple colored complex. The quantitative analysis was performed by spectrophotometry. The qualitative and quantitative formaldehyde analysis methods were applied to 13 bottles of hair straighteners. When submitted to qualitative analysis, all samples showed formaldehyde presence. The quantitative analysis demonstrated that the samples identified as B, C, D, E, G, H, I, J and M presented formaldehyde concentration of 3.5 to 14.5%, which is above of the limit recommended by the National Health Surveillance Agency (ANVISA), of 0.2%. In the label analysis, in all samples were found irregularities.(AU)
Descritores: Rotulagem de Produtos/legislação & jurisprudência
Cosméticos/análise
Formaldeído/análise
Cabelo
-Espectrofotometria/métodos
Indicadores e Reagentes/administração & dosagem
Limites: Humanos
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: biblio-1053200
Autor: Guerrero, Karlo; Arancibia, Alejandra; Caceres, Manuel; Aroca, German.
Título: Release of formaldehyde during the biofiltration of methanol vapors in a peat biofilter inoculated with Pichia pastoris GS115
Fonte: Electron. j. biotechnol;40:10-16, July. 2019. tab, ilus, graf.
Idioma: en.
Projeto: National Comission for Science and Technology (CONICYT) Chile.
Resumo: Background: Methanol can be effectively removed from air by biofiltration. However, formaldehyde is one of the first metabolic intermediates in the consumption of methanol in methylotrophic microorganisms, and it can be released out of the cell constituting a secondary emission. Results: The total removal of methanol was achieved up to input loads of 263 g m−3 h−1 and the maximum elimination capacity of the system was obtained at an empty bed residence times of 90 s and reached 330 g m− 3 h−1 at an input methanol load of 414 g m−3 h−1 and 80% of removal efficiency. Formaldehyde was detected inside the biofilter when the input methanol load was above 212 g m−3 h−1 . Biomass in the filter bed was able to degrade the formaldehyde generated, but with the increase of the methanol input load, the unconsumed formaldehyde was released outside the biofilter. The maximum concentration registered at the output of the system was 3.98 g m−3 when the methanol load was 672 g m−3 h−1 in an empty bed residence times of 60 s. Conclusions: Formaldehyde is produced inside a biofilter when methanol is treated in a biofiltration system inoculated with Pichia pastoris. Biomass present in the reactor is capable of degrading the formaldehyde generated as the concentration of methanol decreases. However, high methanol loads can lead to the generation and release of formaldehyde into the environment
Descritores: Pichia/metabolismo
Metanol/metabolismo
Formaldeído/metabolismo
-Biomassa
Poluentes Atmosféricos
Meio Ambiente
Filtração
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1104336
Autor: Chulibert, María E; Ferrer, Alejo; Koch, Karina E; Rigalli, Alfredo.
Título: Effect of fermented milk with kefir grains on the in vitro demineralization of bovine tooth enamel / Efecto de la leche fermentada con granos de kéfir sobre la desmineralización in vitro del esmalte dental bovino
Fonte: Actual. osteol;15(3):205-213, Sept-Dic. 2019. ilus, tab.
Idioma: en.
Resumo: The dental caries is a progressive destruction of the teeth tissue due to the disbalance in the normal molecule interactions between the enamel and the bio!lm, which alters the demineralization-remineralization process. Milk fermentation produces caseinphosphopeptides with proved remineralizing capacity of the enamel. The presence of these peptides in fermented milk with ke!r grains has been described. The purpose of this work was to evaluate in vitro the capacity of milk ke!r to prevent the demineralization of dental enamel. Bovine incisors (n=68, 17 per group) were treated for 72 h with different solutions: I: artificial saliva at pH 7.2 , II: demineralizing solution at pH 4.5, III: supernatant of kefir fermented milk at pH 4.5, IV: milk supernatant at pH 4.5. The effects of treatments were evaluated by the change in the weight of the specimens, calcium concentration in the solution and by scanning electron microscopy (SEM) of the enamel. Kefir milk supernatant prevented the demineralization process, that was evidenced by a change in weight and calcium concentration that were not different from group I, although the pH was 4.5. In contrast, group IV showed a decrease in weight and an increase in calcium concentration, compared with group I (one way ANOVA, p<0.05). Images of SEM agree with the values of weight and calcium concentration. These results indicate that kefir milk supernatant has a protective effect on enamel demineralization in vitro. (AU)

La caries dental es una patología debido a un desequilibrio en las interacciones moleculares normales entre el esmalte y la biopelícula, que altera el proceso de desmineralización remineralización. La fermentación de la leche produce fosfopéptidos de caseína con probada capacidad remineralizante del esmalte, y se ha descripto la presencia de estos péptidos en la leche fermentada con granos de kéfir. El propósito de este trabajo fue evaluar in vitro la capacidad del kéfir de leche para prevenir la desmineralización del esmalte dental. Sesenta y ocho incisivos bovinos (17 por grupo) fueron tratados durante 72 h con diferentes soluciones: I: saliva artificial, pH 7.2, II: solución desmineralizante, pH 4.5, III: sobrenadante de leche fermentada con kefir, pH 4.5, IV: sobrenadante de leche, pH 4.5. El proceso de desmineralización se evaluó mediante el cambio en el peso de las muestras, la concentración de calcio en la solución y microscopía electrónica de barrido (SEM) del esmalte. El sobrenadante de leche fermentada con kéfir impidió el proceso de desmineralización, que se evidenció por un cambio en el peso y la concentración de calcio que no discreparon del grupo I, a pesar de haber tenido un pH de 4.5. En contraste, el grupo IV mostró una disminución en el peso y un aumento en la concentración de calcio, en comparación con el grupo I (ANOVA a un criterio, p<0.05). Las imágenes SEM concuerdan con los cambios en el peso y la concentración de calcio en los grupos estudiados. Los datos obtenidos demuestran que el sobrenadante de la leche tratada con kéfir tiene un efecto protector sobre la desmineralización del esmalte in vitro, inducida por el pH ácido. (AU)
Descritores: Desmineralização do Dente/prevenção & controle
Kefir/microbiologia
-Saliva Artificial/administração & dosagem
Remineralização Dentária/métodos
Técnicas In Vitro
Bovinos
Caseínas/uso terapêutico
Cálcio/análise
Desmineralização do Dente/patologia
Desmineralização do Dente/terapia
Biofilmes
Cárie Dentária/prevenção & controle
Esmalte Dentário/citologia
Esmalte Dentário/fisiopatologia
Leite/microbiologia
Formaldeído/administração & dosagem
Limites: Animais
Bovinos
Tipo de Publ: Ensaio Clínico Controlado Aleatório Veterinário
Responsável: AR2.1 - Biblioteca Central



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