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Id: biblio-837671
Autor: Carvalho, Juliana Fernandes de; Azevedo, Ítalo Medeiros de; Rocha, Keyla Borges Ferreira; Medeiros, Aldo Cunha; Carriço, Artur da Silva.
Título: Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats
Fonte: Acta cir. bras;32(1):46-55, Jan. 2017. tab, graf.
Idioma: en.
Projeto: CNPq; . CAPES.
Resumo: Abstract Purpose: To evaluate the effect of oxacillin bonded to magnetic nanoparticles in local infection model in rat. Methods: Twelve Wistar rats weighing 290±18g were randomly divided into four groups (n=6, each) and all rats had a magnet ring sutured on their right thighs. In the biodistribution group rats 0.1mL of 99mTc-magnetite (0.66 MBq) was injected i.v and after 30 minutes, biodistribution of 99mTc-magnetite was evaluated in right and left thighs. The other groups were inoculated with MRSA in each thigh muscles. Group 1 rats were injected i.v. with magnetite, group 2 with Magnetite + Oxacillin, group 3 with saline twice a day. After 24 hours samples of muscle secretion were harvested for microbiological analysis; muscle, lungs and kidneys for histology. Results: 99mTc-magnetite uptake was three-fold higher in right thigh muscles (with external magnet) than in the left. In magnetite and oxacillin-magnetite groups, bacterial/CFU was significantly lower in thigh muscles than in saline-controls. The inflammatory reaction in muscles and lungs was significantly lower in oxacillin-magnetite group-rats than in other groups (p<0.001) . Conclusion: This study confirms the potential antimicrobial activity of magnetic nanoparticles for Methicillin-Resistant S. aureus strains, which in addition to concentrate the antibiotic at the infection site, positively influenced the treatment.
Descritores: Oxacilina/administração & dosagem
Infecções Estafilocócicas/tratamento farmacológico
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Nanopartículas de Magnetita/administração & dosagem
Antibacterianos/administração & dosagem
-Distribuição Aleatória
Ratos Wistar
Modelos Animais de Doenças
Nanopartículas
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-1040739
Autor: Amarante, Jarbas F; Ribeiro, Márcia F; Costa, Mateus M; Menezes, Fredson G; Silva, Tania M. S; Amarante, Talita A. B; Gradela, Adriana; Moura, Liliane M. D.
Título: Chemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials / Composição química e atividade antimicrobiana de dois extratos de própolis contra isolados de Staphylococcus spp. e bactérias multirresistentes
Fonte: Pesqui. vet. bras = Braz. j. vet. res;39(9):734-743, Sept. 2019. tab, graf.
Idioma: en.
Projeto: PRONEM-FACEPE.
Resumo: There is a growing need to discover and develop alternative therapies for the treatment of mastitis caused by Staphylococcus spp. and multidrug-resistant bacterial infections. This study examined the chemical composition and antimicrobial potential of two propolis extracts (EPA and EPB) against seventy-seven isolates of Staphylococcus spp. obtained from subclinical bovine mastitis; three clinical strains of MRSA and two from clinical strains of S. aureus ATCC, identified as S. aureus ATCC 6538 and S. aureus ATCC 25923. The total phenolic content was determined by the Folin-Ciocalteau method, the total flavonoid content by the Dowd method and the phenolic profile was quantified by HPLC-DAD. The MBC values of the extracts were evaluated by broth microdilution method. The amount of total phenolic and flavonoid compounds was higher in EPA than EPB. Both extracts revealed the presence of caffeic, coumaric, cinnamic, ferulic and 3,4-dihydroxybenzoic acids, with higher concentrations of coumaric and cinnamic acids. Staphylococcus spp. isolates were susceptible to EPA (90.9%), EPB (83.1%) and oxacillin (80.5%). The oxacillin susceptible isolates were also susceptible to EPA (70.1%) and EPB (80.6%), whereas those oxacillin-resistant strains were also susceptible to EPA (40.0%) and to EPB (26.7%). MBC ranged from 34.3 to 68.7µm/mL for EPA and from 68.7 to 137.5µg/mL for EPB. Both extracts inhibited significantly (100%) the clinical strains of MRSA, S. aureus ATCC 6538 and S. aureus ATCC 25923 at the concentration of 68.7µg/mL. It is concluded that both extracts of propolis, whose main constituents are coumaric and cinnamic acids, have high antimicrobial activity against the microorganisms studied, and EPA also against oxacillin-resistant strains. These findings reinforce its potential use for the treatment of bovine mastitis.(AU)

É cada vez mais oportuna a necessidade de descobrir e desenvolver terapias alternativas para tratamento da mastite causada por Staphylococcus spp. e de infecções bacterianas multirresistentes. Este estudo examinou a composição química e o potencial antimicrobiano de dois extratos etanólicos de própolis (EPA e EPB) contra setenta e sete isolados de Staphylococcus spp. obtidos a partir de mastite bovina subclínica; três estirpes clínicas de MRSA e duas de linhagens clínicas de S. aureus ATCC, identificadas como, S. aureus ATCC 6538 e S. aureus ATCC 25923, ambas metacilina resistentes. O teor total de fenólicos foi determinado pelo método de Folin-Ciocalteau, o teor de flavonoides totais pelo método Dowd e o perfil fenólico foi quantificado por HPLC-DAD. CBM dos extratos foi avaliada pelo método de microdiluição em caldo. A quantidade total de compostos fenólicos e flavonoides foi maior no EPA do que no EPB. Ambos os extratos revelaram a presença dos ácidos cafeico, cumárico, cinâmico, ferúlico e 3,4-di-hidroxibenzóico, com maiores concentrações de ácidos cumárico e cinâmico. Os isolados de Staphylococcus spp. foram sensíveis a EPA (90,9%), EPB (83,1%) e oxacilina (80,5%). Os isolados suscetíveis à oxacilina também foram suscetíveis ao EPA (70,1%) e ao EPB (80,6%), enquanto os do resistente à oxacilina foram suscetíveis ao EPA (40,0%) e ao EPB (26,7%). MBC variou de 34,3 a 68,7µm/mL para EPA e de 68,7 a 137,5µg/mL para EPB. Ambos os extratos inibiram significativamente (100%) as linhagens clínicas de MRSA, S. aureus ATCC 6538 e S. aureus ATCC 25923 na concentração de 68,7µg/mL. Conclui-se que os extratos etanólicos da própolis, cujos principais constituintes são os ácidos cumário e cinâmico, possuem atividade antimicrobiana contra os micro-organismos estudados, e o EPA também contra as cepas resistentes à oxacilina. Estes achados reforçam seu potencial uso para o tratamento da mastite bovina.(AU)
Descritores: Oxacilina
Própole/imunologia
Staphylococcus
Farmacorresistência Bacteriana Múltipla
Compostos Fenólicos/análise
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-841771
Autor: Sued, Bruna Pinto Ribeiro; Pereira, Paula Marcele Afonso; Faria, Yuri Vieira; Ramos, Juliana Nunes; Binatti, Vanessa Batista; Santos, Kátia Regina Netto dos; Seabra, Sérgio Henrique; Hirata Júnior, Raphael; Vieira, Verônica Viana; Mattos-Guaraldi, Ana Luíza; Pereira, José Augusto Adler.
Título: Sphygmomanometers and thermometers as potential fomites of Staphylococcus haemolyticus: biofilm formation in the presence of antibiotics
Fonte: Mem. Inst. Oswaldo Cruz;112(3):188-195, Mar. 2017. tab, graf.
Idioma: en.
Resumo: BACKGROUND The association between Staphylococcus haemolyticus and severe nosocomial infections is increasing. However, the extent to which fomites contribute to the dissemination of this pathogen through patients and hospital wards remains unknown. OBJECTIVES In the present study, sphygmomanometers and thermometers were evaluated as potential fomites of oxacillin-resistant S. haemolyticus (ORSH). The influence of oxacillin and vancomycin on biofilm formation by ORSH strains isolated from fomites was also investigated. METHODS The presence of ORSH on swabs taken from fomite surfaces in a Brazilian hospital was assessed using standard microbiological procedures. Antibiotic susceptibility profiles were determined by the disk diffusion method, and clonal distribution was assessed in pulsed-field gel electrophoresis (PFGE) assays. Minimum inhibitory concentrations (MICs) of oxacillin and vancomycin were evaluated via the broth microdilution method. Polymerase chain reaction (PCR) assays were performed to detect the mecA and icaAD genes. ORSH strains grown in media containing 1/4 MIC of vancomycin or oxacillin were investigated for slime production and biofilm formation on glass, polystyrene and polyurethane catheter surfaces. FINDINGS ORSH strains comprising five distinct PFGE types were isolated from sphygmomanometers (n = 5) and a thermometer (n = 1) used in intensive care units and surgical wards. ORSH strains isolated from fomites showed susceptibility to only linezolid and vancomycin and were characterised as multi-drug resistant (MDR). Slime production, biofilm formation and the survival of sessile bacteria differed and were independent of the presence of the icaAD and mecA genes, PFGE type and subtype. Vancomycin and oxacillin did not inhibit biofilm formation by vancomycin-susceptible ORSH strains on abiotic surfaces, including on the catheter surface. Enhanced biofilm formation was observed in some situations. Moreover, a sub-lethal dose of vancomycin induced biofilm formation by an ORSH strain on polystyrene. MAIN CONCLUSIONS Sphygmomanometers and thermometers are fomites for the transmission of ORSH. A sub-lethal dose of vancomycin may favor biofilm formation by ORSH on fomites and catheter surfaces.
Descritores: Oxacilina/farmacologia
Infecções Estafilocócicas/microbiologia
Infecções Estafilocócicas/transmissão
Termômetros/microbiologia
Vancomicina/farmacologia
Infecção Hospitalar/microbiologia
Biofilmes/crescimento & desenvolvimento
Esfigmomanômetros/microbiologia
Staphylococcus haemolyticus/isolamento & purificação
Staphylococcus haemolyticus/efeitos dos fármacos
Staphylococcus haemolyticus/fisiologia
Antibacterianos/farmacologia
-Resistência a Medicamentos
Testes de Sensibilidade Microbiana
Infecção Hospitalar/transmissão
Eletroforese em Gel de Campo Pulsado
Eletroforese
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-429028
Autor: Adamcryk Wieler, Lorena; Trigoso Agudo, Christian; Vasquez Michel, Aneth; Duran Arias, Loretta.
Título: Determinacion de la Homoresistencia y Heteroresistencia de Staphylococcus Aereus Meticilinoresistente aislado de muestras de pacientes internados en los hospitale Boliviano Holandes, Hospital Obrero y Hospital de Clinicas / Determination of the Homoresistence and Heteroresistence of methicillin resistant staphylococcus aureus isolated from patient admitted to the hospital Boliviano Holandes, Hospital Obrero and Hospital de Clinicas
Fonte: Cuad. Hosp. Clín = Cuad. - Hosp. clín;50(2):7-11, 2005. tab.
Idioma: es.
Resumo: PREGUNTA DE INVESTIGACIÓN ¿Las cepas de Staphylococcus aureus meticilino resistentes circulantes en nuestro medio, serán homoresistentes o heteroresistentes? OBJETIVOS: determinar los fenómenos de homoresistencia y heteroresistencia a meticilina en aislamientos de Staphylococcus aureus de muestras de pacientes internados en los Hospitales Boliviano Holandes, Hospital Obrero y Hospital de Clínicas de la Ciudad de La Paz Determinar el porcentaje de cepas de Staphylococcus aureus resistentes a la meticilina en los aislamientos obtenidos DISEÑO Corte transversal LUGAR Instituto Nacional de Laboratorios en Salud (INLASA) MUESTRAS Las cepas fueron aisladas de muestras de exudados purulentos de abscesos, heridas y quemaduras provenientes de pacientes internados en los Hospitales Boliviano Holandes, Hospital Obrero y Hospital de Clínicas. MÉTODOS: se realizó la identificación de género y especie aureus mediante pruebas de catalasa; coagulasa y manitol respectivamente. La determinación de sensibilidad y /o resistencia se las hizo mediante difusión en disco (Bauer-Kirby) con discos de Oxacilina de 1 µg La Concentración Inhibitoria Mínima (CIM) se la determinó por microdilución en caldo con Oxacilina p.a en polvo RESULTADOS: de 100 aislamientos de Staphylococcus aureus, el 16% son resistentes a la meticilina En ninguna de las cepas resistentes se pudo evidenciar homoresistencia a la meticilina, por tanto el 100% de las cepas son heteroresistentes. CONCLUSIONES: se determina que en nuestro medio la meticilina mantiene una buena actividad antimicrobiana frente a Staphylococcus aureus. No existe el fenómeno de homoresistencia en las cepas aisladas en este estudio.

RESEARCH QUESTION Are the circulating methicillin resistant staphylococcus aureus strains in our patients homoresistant or heteroresistant? OBJECTIVES: To assess the homoresistance and heteroresistance to methicillin of Staphylococcus aureus isolated from purulent exudate samples obtained from patients admitted to the Hospital Boliviano Holandes, Hospital Obrero and Hospital de Clinicas in La Paz. To determine the percentage of methicillin resistant Staphylococcus aureus in these samples. Study Design Cross sectional Study Location Instituto Nacional de Laboratorios de Salud (INLASA) Samples The strains were isolated from samples of purulent discharges from abscesses, wounds, infected burns from patients admitted to the Hospital Boliviano Holandes, Hospital Obrero, and Hospital de Clinicas. METHODS: Staphylococcus aureus was identified by tests of catalase, coagulase and manitol. Sensitivity and/or resistance was determined by disc diffusion (Bauer Kirby) with discs of Oxacilina of 1 µg The Minimal Inhibitory Concentration (CIM) was determined by microdilution. RESULTS: of 100 Staphylococcus aureus isolations, 16% were methicillin resistant. None of these strains was shown to be homoresistant. Therefore, we assume that 100% of the strains were heteroresistant. CONCLUSIONS: methicillin maintains a good antimicrobial activity against Staphylococcus aureus. The isolations in our study did not show homoresistance.
Descritores: Staphylococcus aureus
Meticilina
-Oxacilina
Catalase
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Relatos de Casos
Responsável: BO6.1 - Biblioteca


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Id: lil-795373
Autor: Horna, Gertrudis; Molero, María L; Benites, Liliana; Roman, Sigri; Carbajal, Luz; Mercado, Erik; Castillo, María E; Zerpa, Rito; Chaparro, Eduardo; Hernandez, Roger; Silva, Wilda; Campos, Francisco; Saenz, Andy; Reyes, Isabel; Villalobos, Alex; Ochoa, Theresa J.
Título: Oxacillin disk diffusion testing for the prediction of penicillin resistance in Streptococcus pneumoniae / Prueba de difusión con discos de oxacilina para predecir la resistencia a la penicilina de Streptococcus pneumoniae
Fonte: Rev. panam. salud pública = Pan am. j. public health;40(1):57-63, Aug. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT Objective To 1) describe the correlation between the zones of inhibition in 1-µg oxacillin disk diffusion (ODD) tests and penicillin and ceftriaxone minimum inhibitory concentrations (MICs) of meningeal and non-meningeal strains of Streptococcus pneumoniae and 2) evaluate the usefulness of the ODD test as a predictor of susceptibility to penicillin in S. pneumoniae and as a quick and cost-effective method easily implemented in a routine clinical laboratory setting. Methods S. pneumoniae isolates from healthy nasopharyngeal carriers less than 2 years old, obtained in a multicentric cross-sectional study conducted in various Peruvian hospitals and health centers from 2007 to 2009, were analyzed. Using Clinical and Laboratory Standards Institute (CLSI) breakpoints, the correlation between the zones of inhibition of the ODD test and the MICs of penicillin and ceftriaxone was determined. Results Of the 571 S. pneumoniae isolates, 314 (55%) showed resistance to penicillin (MIC ≥ 0.12 µg/mL) and 124 (21.7%) showed resistance to ceftriaxone (MIC ≥ 1 µg/mL). Comparison of the ODD test zones of inhibition and the penicillin MICs, using the CLSI meningeal breakpoints, showed good correlation (Cohen’s kappa coefficient = 0.8239). Conclusions There was good correlation between ODD zones of inhibition and penicillin meningeal breakpoints but weak correlation between the ODD results and non-meningeal breakpoints for both penicillin and ceftriaxone. Therefore, the ODD test appears to be a useful tool for predicting penicillin resistance in cases of meningeal strains of S. pneumoniae, particularly in low- and middle- income countries, where MIC determination is not routinely available.

RESUMEN Objetivo 1) Describir la correlación entre las zonas de inhibición observadas en la prueba de difusión con discos de oxacilina de 1 µg y la concentración inhibitoria mínima (CIM) de penicilina y ceftriaxona frente a cepas meníngeas y no meníngeas de Streptococcus pneumoniae y 2) evaluar si la prueba de difusión con discos de oxacilina permite predecir la sensibilidad de S. pneumoniae a la penicilina y sirve como método rápido y eficaz en función de los costos, y resulta fácil de aplicar en los laboratorios clínicos ordinarios. Métodos Se analizaron colonias de S. pneumoniae aisladas de la nasofaringe de portadores sanos menores de 2 años obtenidas en un estudio transversal multicéntrico realizado en diversos hospitales y centros de salud del Perú entre los años 2007 y 2009. Se determinó la correlación entre las zonas de inhibición observadas en la prueba de difusión con discos y la CIM de la penicilina y la ceftriaxona utilizando los valores críticos definidos por el Instituto de Estándares Clínicos y de Laboratorio. Resultados De las 571 colonias aisladas de S. pneumoniae, 314 (55 %) presentaron resistencia a la penicilina (CIM ≥ 0,12 µg/ml) y 124 (21,7%), resistencia a la ceftriaxona (CIM ≥ 1 µg/ml). Se observó una buena correlación (coeficiente κ de Cohen = 0,8239) entre las zonas de inhibición de la prueba de difusión con discos y la CIM de la penicilina utilizando los valores críticos del Instituto respecto de las cepas meníngeas. Conclusiones Se encontró una buena correlación entre las zonas de inhibición de la prueba de difusión con discos y los valores críticos de CIM de la penicilina respecto de las cepas meníngeas, pero una correlación débil entre los resultados de la prueba de difusión y los valores críticos tanto de la penicilina como de la ceftriaxona respecto de las cepas no meníngeas. Por consiguiente, la prueba de difusión con discos es un método de utilidad para predecir la resistencia a la penicilina de las cepas meníngeas de S. pneumoniae, en particular en los países de ingresos bajos y medianos, donde no suele ser posible determinar la CIM.
Descritores: Oxacilina/administração & dosagem
Penicilinas/uso terapêutico
Infecções Pneumocócicas/tratamento farmacológico
Resistência Microbiana a Medicamentos
Responsável: BR1.1 - BIREME


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Id: biblio-976423
Autor: Scherer, Carolina B; Botoni, Larissa S; Carvalho, Antônio U; Keller, Kelly M; Costa-Val, Adriane P.
Título: Ceftaroline resistance in Staphylococcus pseudintermedius gene mecA carriers / Resistência à ceftarolina em Staphylococcus pseudintermedius portadores do gene mecA
Fonte: Pesqui. vet. bras = Braz. j. vet. res;38(12):2233-2236, dez. 2018. tab.
Idioma: en.
Resumo: Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) being a constant concern, ceftaroline fosamil has been recently approved as a new cephalosporin, active against MRSA, for use in humans; only rare cases of resistance have been reported till date. There is no report of resistance to ceftaroline in Staphylococcus pseudintermedius, which is the main bacterium causing dermatitis and otitis in dogs. To evaluate staphylococcal resistance to ceftaroline, 35 isolates of methicillin-resistant S. pseudintermedius (MRSP), carrying the mecA gene, from 26 dogs with folliculitis and nine dogs with external otitis, underwent disk diffusion test with cefoxitin, oxacillin, and ceftaroline. Tests with cefoxitin and oxacillin showed > 90% sensitivity in methicillin resistance detection. In the disk diffusion test, 97.14% (34/35) were resistant to cefoxitin, 94.29% (33/35) to oxacillin, and 31.43% (11/35) to ceftaroline. Of the ceftaroline-resistant strains, 27.27% (3/11) were obtained from the ears of dogs while the rest (8/11) were from the skin. The current report is the first description of MRSP resistance to ceftaroline.(AU)

Infecções causadas por Staphylococcus aureus resistente à meticilina (MRSA) são uma preocupação médica constante. A ceftarolina fosamila é uma nova cefalosporina ativa contra Staphylococcus aureus resistente à meticilina recentemente aprovada para uso em humanos e raros casos de resistência relatados até agora. Não há relatos de resistência à ceftarolina em Staphylococcus pseudintermedius, principal bactéria causadora de dermatite e otite em cães. Com o objetivo de avaliar a resistência estafilocócica à ceftarolina, 35 amostras de S. pseudintermedius resistentes à meticilina (MRSP), portadoras do gene mecA, provenientes de 26 cães com foliculite e 9 com otite externa foram submetidos ao teste de disco-difusão com cefoxitina, oxacilina e ceftarolina. Os testes realizados com cefoxitina e oxacilina mostraram mais de 90% de sensibilidade na detecção da resistência à meticilina em ambas. No teste da disco-difusão, 97,14% (1/35) foram resistentes à cefoxitina, 94,29% (3/35) à oxacilina e 31,43% (11/35) à ceftarolina. Das cepas resistentes às ceftarolina, 27,27 (3/11) foram provenientes de ouvido de cães e as demais (8/11), provenientes da pele, sendo essa primeira descrição de resistência de MRSP à ceftarolina na literatura atual.(AU)
Descritores: Oxacilina
Staphylococcus/genética
Staphylococcus aureus
Infecções Cutâneas Estafilocócicas/veterinária
Cefoxitina
Resistência às Cefalosporinas
Cães/microbiologia
-Dermatite/veterinária
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão/veterinária
Foliculite/veterinária
Limites: Animais
Cães
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-281711
Autor: Peñaloza, Jorge; Sojo, Ernesto; Caletti, María G; Mendilaharzu, Fernando.
Título: Valoración de un nuevo esteroide (deflazacort) en el tratamiento inicial en niños con síndrome nefrótico idiopático / Evaluation of deflazacort, a new steroid, in the initial theraphy of children with idiopathic nephrotic syndrome
Fonte: Med. infant;1(4,n.esp):185-189, jun. 1994. tab, graf.
Idioma: es.
Resumo: Evaluamos un nuevo derivado oxazolínico de la prednisona comparando su respuesta y efectos secundarios con los observados en el tratamiento clásico con prednisona, en un grupo de 24 niños durante el primer episodio de Síndrome Nefrótico Idiopático. Grupo A: 12 niños de edad media: 43 meses (r: 10 á 72 m) recibieron deflazacort en dosis de 1.8 á 2.2 mg/kg/día durante 30 días contínuos y 30 días alternos (serie completa). Grupo B: 12 niños, edad media: 37 meses (r: 29 á 70) recibieron metilprednisona a la dosis de 1.5 á 1.7 mg/kg/día con igual esquema que los del grupo A. En 6 pacientes del Grupo A la proteinuria desapareció entre los 7 y 26 días de tratamiento (x 13.8 d). Los otros 6 no negativizaron la proteinuria. En 7 niños del Grupo B la proteinuria remitió entre 5 y 15 días (x 10.5 d), los 5 restantes persistieron con proteinuria masiva hasta la finalización de la primera serie de tratamiento. Los parámetros bioquímicos pre y post tratamiento en los pacientes corticosensibles no mostraron diferencias significativas entre ambos grupos. Los valores de colesterol fueron menores en los que recibieron deflazacort aunque sin significancia estadística. Los efectos esteroides colaterales estuvieron presentes en la mayoría de los niños tratados con prednisona, y fueron significativamente menores en los que recibieron deflazacort
Descritores: Oxacilina/administração & dosagem
Oxacilina/efeitos adversos
Esteroides/administração & dosagem
Esteroides/efeitos adversos
Prednisona/administração & dosagem
Prednisona/efeitos adversos
Síndrome Nefrótica/terapia
-Argentina
Limites: Humanos
Masculino
Feminino
Pré-Escolar
Tipo de Publ: Relatos de Casos
Responsável: AR305.1 - SID - Servicio de Información y Documentación


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Id: biblio-884032
Autor: Boriollo, Marcelo Fabiano Gomes; Rodrigues Netto, Manoel Francisco; Silva, Jeferson Júnior da; Silva, Thaísla Andrielle da; Castro, Maysa Eduarda de; Elias, Júlio César; Höfling, José Francisco.
Título: Isoenzyme genotyping and phylogenetic analysis of oxacillin-resistance Staphylococcus aureus isolates
Fonte: Braz. j. oral sci;16:e17052, jan.-dez. 2017. tab.
Idioma: en.
Resumo: Aim: The propagation of S. aureus in hospital and dental environments is considered an important public health problem since resistant strains can cause serious infections in humans. The genetic variability of 99 oxacillin-resistant S. aureus isolates (ORSA) from the dental patients (oral cavity) and environments (air) was studied by isoenzyme genotyping. Methods: S. aureus isolates were studied using isoenzyme markers (alcohol dehydrogenase, sorbitol dehydrogenase, mannitol-1-phosphate dehydrogenase, malate dehydrogenase, glucose dehydrogenase, D-galactose dehydrogenase, glucose-6-phosphate dehydrogenase, catalase and α/ß-esterase) and genetic (Nei's statistics) and cluster analysis (UPGMA algorithm). Results: A highly frequent polyclonal pattern was observed in this population of ORSA isolates, suggesting various sources of contamination or microbial dispersion. Genetic relationship analysis showed a high degree of polymorphism between the strains, and it revealed three taxa (A, B and C) distantly genetically related (0.653≤dij≤1.432) and fifteen clusters (I to XV) moderately related (0.282≤dij<0.653). These clusters harbored two or more highly related strains (0≤dij<0.282), and the existence of microevolutionary processes in the population of ORSA. Conclusion: This research reinforces the hypothesis of the existence of several sources of contamination and/or dispersal of ORSA of clinical and epidemiologically importance, which could be associated with carriers (patients) and dental environmental (air) (AU)
Descritores: Ar
Consultórios Odontológicos
Isoenzimas
Boca
Oxacilina
Staphylococcus aureus
-Técnicas de Genotipagem
Responsável: BR218.1 - Biblioteca Carlos Henrique Robertson Liberalli


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Id: biblio-842031
Autor: Botoni, Larissa S; Scherer, Carolina B; Silva, Rodrigo O; Coura, Fernanda M; Heinemann, Marcos B; Paes-Leme, Fabiola O; Costa-Val, Adriane P.
Título: Prevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyoderma / Prevalência e suscetibilidade in vitro de Staphylococcus pseudintermedius resistente à meticilina (MRSP) oriundos de pele e narinas de cães com piodermite superficial
Fonte: Pesqui. vet. bras = Braz. j. vet. res;36(12):1178-1180, Dec. 2016.
Idioma: en.
Resumo: In order to assess the prevalence of Methicillin-resistant Staphylococcus pseudintermedius from skin and nostrils of dogs with pyoderma, to determine its in vitro susceptibility, and to correlate these data with the presence of the mecA gene, 43 dogs were selected. Samples were collected from secretion of their skin lesions and right nostril, cultured, and analyzed for phenotypic and genotypic characteristics of the bacteria studied. In 62 samples (91%) the microorganism was classified as S. pseudintermedius. The rate of resistance against antibiotics ranged from 7% (amikacin; 4/62) to 77% (sulfamethoxazole + trimethoprim; 48/62). Resistance against oxacillin was found in 34% of the samples (21/62). Twenty-five samples (37%) were strains that carried the mecA gene. A significant correlation (P<0.01) was found between presence of the mecA gene and oxacillin resistance. Seventeen dogs were mecA gene carriers, and 8 (47%) of them had the gene in the skin lesions and nostril. A significant correlation (P<0.01) was also observed between the presence of mecA gene in the skin lesions and nostrils. Oxacillin resistance in vitro can be safely used to indicate the presence of mecA gene in MRSP samples. The nostrils can be a reservoir of MRSP in dogs.(AU)

Para acessar a prevalência de MRSP na pele e nas narinas de cães com piodermite superficial, determinar a suscetibilidade in vitro, e correlacionar estes dados com a presença do gene mecA, foram selecionados 43 cães. Amostras de lesões de pele e narinas foram coletadas, cultivadas, e analisadas fenotipica e genotipicamente. Em 62 amostras (91%), os microrganismos foram classificados como Staphylococcus pseudintermedius. A taxa de resistência a antibióticos variou entre 7% (amicacina; 4/62) e 77% (sulfamethoxazol + trimetoprim; 48/62). Resistência a oxacilina foi observada em 34% das amostras (21/62). Vinte e cinco amostras (37%), eram cepas portadoras do gene mecA. Correlação significativa (P<0,01) foi observada entre a presença do gene mecA e a resistência à oxacilina. Considerando os cães, 17 eram portadores de cepas com gene mecA e 8(47%) delas carreavam este gene nas amostras de lesão de pele e nas narinas. Correlação significativa (P>0,01) foi observada entre a presença do gene mecA nas lesões de pele e nas narinas. Sendo assim, resistência à oxacilina in vitro pode ser aferida com segurança para indicar a presença do gene mecA em amostras de MRSP, e as narinas podem constituir em um reservatório dos microorganismos em cães.(AU)
Descritores: Resistência a Meticilina
Oxacilina/análise
Pioderma/veterinária
Infecções Estafilocócicas/epidemiologia
Staphylococcus
-Antibacterianos
Técnicas In Vitro/veterinária
Limites: Animais
Cães
Responsável: BR1.1 - BIREME


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Id: biblio-835526
Autor: Serrano, AL; Sierra, MA; Ovalle, AL; Saucedo, MA; Gálvez, AE.
Título: Determinación de staphylococcus aureus meticilino resistente de la comunidad (Sarm-com) en dos hospitales de Guatemala / Determination of methicillin resistant staphylococcus aureus community (Sarm-com) in two hospitals in Guatemala
Fonte: Rev. Col. Méd. Cir. Guatem;6(3[2?]):62-67, jul.-dic. 2011. graf.
Idioma: es.
Resumo: El objetivo de este trabajo fue determinar cepas de Stapylococcus aureus meticilino resistente de la comunidad (SARM-com) en aislamientos provenientes de infecciones de la piel de pacientes del Hospital Roosevelt y hospital nacional Pedro de Betancourt de Guatemala. Para ello se realizó un estudio exploratorio de tipo descriptivo el cual consistió en un muestreo de 12 semanas en el laboratorio de microbiología del hospital Roosevelt y del hospital nacional Pedro de Betancourt. Se recolectaron las cepas que cumplieron con los siguientes criterios: haber sido identificadas como S. aureus, que presentaran resistencia a todos los betalactámicos, por medio de la resistencia a oxacilina y como resistencia variable a macrólidos y lincosamidas...
Descritores: Guatemala
Hospitais
Lincosamidas/uso terapêutico
Macrolídeos/uso terapêutico
Oxacilina/efeitos adversos
Staphylococcus aureus/imunologia
Limites: Humanos
Responsável: GT5.1 - Biblioteca y Centro de Documentación Dr. Julio de León Méndez



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