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Id: biblio-836667
Autor: Sá Dias, Tania Cristina de.
Título: Avaliação in vitro do efeito de diferentes processos de alisamento químico/térmico na fibra capilar / In vitro evaluation of the straightening effect by different chemical/thermal processes in the hair fiber.
Fonte: São Paulo; s.n; abr. 2015. 198 p. tab, graf, ilus.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo para obtenção do grau de Doutor.
Resumo: A aparência dos cabelos é de fundamental importância na sociedade atual. Estando em moda, cabelos mais lisos e com menos volume, os consumidores que antes os alisavam com produtos químicos e força mecânica, passaram a utilizar um tratamento térmico, além do secador de cabelos: as piastras ("chapinhas") que atuam em valores de temperatura ao redor de 230°C. Esse procedimento ocasiona além dos danos mecânicos e químicos também dano térmico, tornando os cabelos ainda mais fragilizados. O escopo deste estudo foi avaliar o dano na fibra capilar, de amostras não tratadas e nas que receberam aplicação de alisantes/relaxantes tradicionais e alternativos. O estudo foi dividido em cinco capítulos que avaliam: aplicação dos alisantes/relaxantes com ingredientes ativos distintos; danos mecânicos, perda Protéica; análise térmica e microscopia eletrônica de varredura. As amostras de cabelo utilizadas em todos os estudos foram tratadas como descrito no primeiro capítulo. Foram aplicados produtos comerciais contendo os seguintes ingredientes ativos: Hidróxido de Sódio, Tioglicolato de Amônio, Hidróxido de Guanidina (reação de hidróxido de cálcio com carbonato de guanidina), formaldeído e ácido glioxílico isolado e em combinação com carbocisteína. O uso de formaldeído e ácido glioxilico em formulações de alisantes/relaxantes está proibido pela Agência Nacional de Vigilância Sanitária. Todos os produtos aplicados alisaram os cabelos; os procedimentos que utilizaram a piastra tornaram os fios mais lisos. Os alisantes/relaxantes à base de ácido glioxilico e formaldeído reduziram de forma expressiva a tensão de ruptura dos cabelos tornando-os mais frágeis. A maior perda protéica foi observada na amostra tratada com carbocisteína (1,74 mg/g cabelo). Nos estudos de análise térmica, na fase de desidratação a amostra tratada com carbocisteína apresentou maior perda de massa (15,17%); na fase de denaturação da proteína, a tratada com hidróxido de sódio (51,06%); e na fase de eliminação do material carbonáceo, todas as amostras apresentaram perda de massa maior que a amostra não tratada; as menores temperaturas de pico foram as das amostras sem tratamento alisante (630°C) e ácido glioxílico (640°C). Observando-se as imagens de microscopia eletrônica nota-se modificação nas bordas das cutículas das amostras indicando que sofreram agressão; o hidróxido de guanidina deixou adicionalmente resíduo; as amostras tratadas com ácido glioxílico e formaldeído apresentaram a formação de filme superficial como um "envelopamento" da fibra. Os resultados sugerem que não há predominância de um procedimento mais danoso que os demais; porém os que utilizaram a piastra (alisamentos/relaxamento ácidos) acentuaram os danos

The appearance of the hair is of fundamental importance in today's society. Being in fashion, hair straight and with less volume, consumers that before straighted hair with chemicals products and mechanical strength began to use a heat treatment, in addition to hair dryers: the hot plates ("chapinhas") acting on temperature values around 230°C. This procedure causes not only mechanical and chemical damage but also thermal one, making the hair more fragile. The scope of this study was to evaluate the damage to the hair fiber, in untreated samples and these receiving straighteners/relaxers application of traditional and alternative products.The study was divided into five chapters that evaluated: application of straighteners/relaxers with different active ingredients; mechanical damage, protein loss; thermal analysis and scanning electron microscopy. The hair samples used in all studies were treated as described in the chapter one. Commercial products containing the following active ingredients were used: Sodium Hydroxide, Ammonium Thioglycolate, Guanidine Hydroxide (calcium hydroxide reaction with guanidine carbonate), Formaldehyde and Glyoxylic Acid alone and in combination with Carbocysteine. The use of Formaldehyde andGlyoxylicAcid in straightening/relaxing formulations are prohibited by the National Agency for Sanitary Vigilance. All applied products, straight the hair samples; the procedures that used the hot plates become the hair more straight. The straightening/relaxing based on Glyoxylic Acid and Formaldehyde reduced significantly the hair break point making them more fragile. Most protein loss was observed in the sample treated with Carbocysteine (1.74mg/g hair).In the thermal analysis studies at the dewatering stage, Carbocystein treated samples showed greater weight loss(15.17%), at the protein denaturation stage this treated with Sodium Hydroxide (51.06%) and in the carbonaceous material elimination phase all samples showed mass loss greater than the untreated sample;. The lower peak temperatures were observed in the samples without treatment (630°C) and with Glyoxylic Acid (640°C). Observing the images of electron microscopy is noted the change in the cuticle aspect of the samples showing that the edges were damaged, Guanidine Hydroxide, left further residue: the samples treated with Glyoxylic Acid and Formaldehyde showed the formation of surface film as an "enveloping" fiber. The results suggest that there is not a predominance of a more harmful treatment than other, but those using hot plates(straightening/relaxing acids) emphasize the damage
Descritores: Estudos de Avaliação como Assunto/análise
Preparações para Cabelo/efeitos adversos
Cabelo/química
Tratamento Térmico
-Cosméticos
Formaldeído
Guanidina
Técnicas In Vitro/instrumentação
Hidróxido de Sódio/efeitos adversos
Tioglicolatos/efeitos adversos
Tipo de Publ: Técnicas In Vitro
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas
BR40.1; T668.55, S125a. 30100022209-F


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Texto completo SciELO Venezuela
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Id: lil-631419
Autor: Torres-Cepeda, Duly; Guerra-Velásquez, Mery; Reyna-Villasmil, Eduardo; Colmenares-Vega, María; Delgado-Delgado, Oneida; Mejía-Montilla, Jorly; Reyna-Villasmil, Nadia.
Título: Tocólisis con clorhidrato de isoxuprina o nifedipina en la amenaza de parto pretérmino / Tocolysis with isoxuprine hydrochloride or nifedipine in preterm labor
Fonte: Rev. obstet. ginecol. Venezuela;70(1):11-17, mar. 2010. tab.
Idioma: es.
Resumo: Comparar la eficacia del clorhidrato de isoxuprina o la nifedipina en la tocólisis de la amenaza de parto pretérmino. Se seleccionaron 82 pacientes con edad gestacional entre 24 y 34 semanas y diagnóstico de amenaza de parto pretérmino. Las pacientes se dividieron al azar en 2 grupos para recibir clorhidrato de isoxuprina (grupo A) o nifedipina (grupo B). Se determinaron el tiempo de cese de las contracciones, tensión arterial materna, concentraciones de glucosa y efectos adversos maternos. Maternidad "Dr. Nerio Belloso", Hospital Central "Dr. Urquinaona", Maracaibo. Estado Zulia. Se logró una tocólisis efectiva en las primeras 24 horas en 61,0 por ciento y 70,7 por ciento de las pacientes del grupo A y B, respectivamente (P = ns). Después de 7 días de tratamiento, 36,6 por ciento de las pacientes en el grupo A y 31,7 por ciento de las pacientes en el grupo B aun permanecían sin contracciones (P = ns). Se logró un retraso del parto hasta las 34 semanas o más en 26,8 por ciento y 29,3 por ciento de las pacientes de los grupos A y B, respectivamente. En el grupo de pacientes tratadas con clorhidrato de isoxuprina se observó un aumento significativo de las concentraciones séricas de glucosa (P < 0,001). Los efectos adversos maternos fueron significativamente más frecuentes en el grupo de clorhidrato de isoxuprina después de 2 y 24 horas de tratamiento (P < 0,05). La nifedipina es igual de efectiva que el clorhidrato de isoxuprina en la tocólisis de la amenaza de parto pretérmino y produce menos efectos adversos

To compare the efficacy of isoxuprine clorhidrate or nifedipine in tocolysis of threatened preterm labor. 82 patients with a gestational age between 24 and 34 weeks and threatened preterm labor diagnosis were selected. Patients were randomly divided in 2 groups to receive isoxuprine clorhidrate (group A) or nifedipine (group B). Time of cease of contractions, maternal blood pressure, glucose concentrations and maternal adverse effects were determined. Maternidad "Dr. Nerio Belloso", Hospital Central "Dr. Urquinaona", Maracaibo. Estado Zulia. An effective tocolysis was obtained within 24 hours in 61.0 percent and 70.7 percent for patients in group A and B, respectively (P = ns). After 7 days of treatment, 36.6 percent of patients in group A and 31,7 percent of patients in group B were still without contractions (P = ns). A delay in labor till 34 weeks or more was made in 26.8. percent and 29.3 percent of patients in group A and B, respectively. In the group of patients treated with isoxuprine clorhidrate a significant raise of glucose concentrations was observed (P < 0.001). Maternal adverse effects were significant more frequent in isoxuprine clorhidrate group after 2 and 24 hours of treatment (P < 0,05). Nifedipine has a similar effectivity than isoxuprine clorhidrate for tocolysis in threatened preterm labor and produces less adverse effects
Descritores: Guanidina/efeitos adversos
Isoxsuprina/efeitos adversos
Nifedipino/efeitos adversos
Trabalho de Parto Prematuro
Tocólise/efeitos adversos
Tocólise/métodos
-Cuidado Pré-Natal
Limites: Humanos
Feminino
Gravidez
Tipo de Publ: Estudo Comparativo
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Texto completo SciELO Brasil
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Id: lil-304654
Autor: Suffys, Philip; Vanderborght, Patricia Rosa; Santos, Patricia Barros dos; Correa, Leticia Almeida Pinto; Bravin, Yolanda; Kritski, Afranio Lineu.
Título: Inhibition of the polymerase chain reaction by sputum samples from tuberculosis patients after processing using a silica-guanidiniumthiocyanate DNA isolation procedure
Fonte: Mem. Inst. Oswaldo Cruz;96(8):1137-1139, Nov. 2001.
Idioma: en.
Resumo: With the objective to evaluate PCR-mediated detection of Mycobacterium tuberculosis DNA as a diagnostic procedure for diagnosis of tuberculosis in individuals attending ambulatory services in Primary Health Units of the City Tuberculosis Program in Rio de Janeiro, Brazil, their sputum samples were collected and treated with a DNA extraction procedure using silica-guanidiniumthiocyanate. This procedure has been described to be highly efficient for extraction of different kind of nucleic acids from bacteria and clinical samples. Upon comparing PCR results with the number of acid-fast bacilli, no direct relation was observed between the number of bacilli present in the sample and PCR positivity. Part of the processed samples was therefore spiked with pure DNA of M. tuberculosis and inhibition of the PCR reaction was verified in 22 out of 36 (61 percent) of the samples, demonstrating that the extraction procedure as originally described should not be used for PCR analysis of sputum samples
Descritores: DNA Bacteriano
Mycobacterium tuberculosis
Reação em Cadeia da Polimerase
Escarro
Tuberculose Pulmonar
-Desinfetantes
Guanidina
Mycobacterium tuberculosis
Sensibilidade e Especificidade
Tiocianatos
Limites: Humanos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: lil-303884
Autor: Araya, Pamela; Rosemblatt, Mario; Valenzuela, Pablo; Murialdo, Helios.
Título: The bacteriophage lambda DNA packaging enzyme: identification of four structural domains of the gpNu1 subunit using limited proteolysis
Fonte: Biol. Res;34(3/4):207-216, 2001. tab, graf.
Idioma: en.
Resumo: Lambda DNA terminase, the enzyme that cleaves virion-length chromosomes from multigenomic concatemers and packages them into the bacteriophage head, is composed of two subunits, gpNu1 and gpA. Direct determination of the structure of gpNu1, the smaller subunit, has not been possible because of its insolubility in aqueous solutions. Therefore, to identify smaller and potentially water-soluble domains of gpNu1, we analyzed the nature of the products obtained by limited digestion of the protein with several proteases. The gpNu1 subunit was obtained from E. coli cells transfected with the plasmid pH6-Nu1 that overproduces the protein. Incubation of gpNu1 solubized in 2.5 M guanidinium chloride with chymotrypsin resulted in the formation of at least eight discrete protein bands, while treatment with endoproteinase glu-C and bromelain yielded three and one major bands, respectively. The peptides generated by digestion with the various proteases were separated by two-dimensional gel electrophoresis and transferred to Immobilon membranes. Amino acid sequencing of the peptides allowed for the precise assignment of their N-terminal amino acid, while their estimated molecular weights permitted the identification of their C-terminal ends. The results reveal that in the presence of 2.5 M guanidinium chloride, gpNu1 is partially folded in at least four distinct structural domains that correspond to functional domains as determined by previously reported genetic experiments. This information is key to design new plasmids to overproduce these domains for further structural analysis.
Descritores: Bacteriófago lambda
Quimotripsina
DNA Viral
Proteínas Virais
-Sequência de Aminoácidos
Bacteriófago lambda
Eletroforese em Gel Bidimensional
Eletroforese em Gel de Poliacrilamida
Guanidina
Peso Molecular
Peptídeo Hidrolases
Dobramento de Proteína
Estrutura Terciária de Proteína
Proteínas Virais
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
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Id: lil-233699
Autor: Martins, N. F; Santoro, M. M.
Título: Partially folded intermediates during trypsinogen denaturation
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;32(6):673-82, Jun. 1999. graf.
Idioma: en.
Resumo: The equilibrium unfolding of bovine trypsinogen was studied by circular dichroism, differential spectra and size exclusion HPLC. The change in free energy of denaturation was delta GH2O = 6.99 + ou - 1.40 kcal/mol for guanidine hydrochloride and delta GH2O = 6.37 + ou - 0.57 kcal/mol for urea. Satisfactory fits of equilibrium unfolding transitions required a three-state model involving an intermediate in addition to the native and unfolded forms. Size exclusion HPLC allowed the detection of an intermediate population of trypsinogen whose Stokes radii varied from 24.1 + ou - 0.4 angstron to 26.0 + ou - 0.3 angstron 1.5 M and 2.5 M guanidine hydrochloride, respectively. During urea denaturation, the range of Stokes radii varied from 23.9 + ou - 0.3 angstron to 25.7 + ou - 0.6 angstron for 4.0 M and 6.0 M urea, respectively. Maximal intrinsic fluorescence was observed at about 3.8 M urea with 8-aniline-1-naphthalene sulfonate (ANS) binding. These experimental data indicate that the unfolding of bovine trypsinogen is not a simple transition and suggest that the equilibrium intermediate population comprises one intermediate that may be characterized as a molten globule. To obtain further insight by studying intermediates representing different stages of unfolding, we hope to gain a better understanding of the complex interrelations between protein conformation and energetics.
Descritores: Dobramento de Proteína
Tripsinogênio/metabolismo
-Cromatografia Líquida de Alta Pressão
Diuréticos Osmóticos/farmacologia
Guanidina/farmacologia
Parassimpatomiméticos/farmacologia
Desnaturação Proteica
Ureia/farmacologia
Limites: Animais
Bovinos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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