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Pesquisa : D02.172 [Categoria DeCS]
Referências encontradas : 31 [refinar]
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Id: biblio-1087520
Autor: Sarkar, Shrabana; Banerjee, Aparna; Chakraborty, Nibedita; Soren, Karuna; Chakraborty, Priyanka; Bandopadhyay, Rajib.
Título: Structural-functional analyses of textile dye degrading azoreductase, laccase and peroxidase: a comparative in silico study
Fonte: Electron. j. biotechnol;43:1-7, Jan. 2020. tab, graf, ilus.
Idioma: en.
Resumo: Background: Textile industry not only plays a vital role in our daily life but also a prominent factor in improving global economy. One of the environmental concern is it releases huge quantities of toxic dyes in the water leading to severe environmental pollution. Bacterial laccase and azoreductase successfully oxidize complex chemical structure of nitrogen group-containing azo dyes. Additionally, the presence of textile dye infuriates bacterial peroxidase to act as a dye degrading enzyme. Our present study deals with three textile dye degrading enzymes laccase, azoreductase, and peroxidase through analyzing their structural and functional properties using standard computational tools. Result: According to the comparative analysis of physicochemical characteristics, it was clear that laccase was mostly made up of basic amino acids whereas azoreductase and peroxidase both comprised of acidic amino acids. Higher aliphatic index ascertained the thermostability of all these three enzymes. Negative GRAVY value of the enzymes confirmed better water interaction of the enzymes. Instability index depicted that compared to laccase and preoxidase, azoreductase was more stable in nature. It was also observed that the three model proteins had more than 90% of total amino acids in the favored region of Ramachandran plot. Functional analysis revealed laccase as multicopper oxidase type enzyme and azoreductase as FMN dependent enzyme, while peroxidase consisted of α-ß barrel with additional haem group. Conclusion: Present study aims to provide knowledge on industrial dye degrading enzymes, choosing the suitable enzyme for industrial set up and to help in understanding the experimental laboratory requirements as well.
Descritores: Compostos Azo/metabolismo
Peroxidase/química
Lacase/química
NADH NADPH Oxirredutases/química
-Temperatura
Compostos Azo/química
Indústria Têxtil
Biodegradação Ambiental
Simulação por Computador
Estabilidade Enzimática
Peroxidase/metabolismo
Lactase/metabolismo
Corantes/metabolismo
NADH NADPH Oxirredutases/metabolismo
Tipo de Publ: Estudo Comparativo
Responsável: CL1.1 - Biblioteca Central


  2 / 31 LILACS  
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Texto completo SciELO Brasil
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Id: biblio-1010871
Autor: Luo, Wei-wei; Zhou, Xue-ling; Wang, Qing-qing; Shao, Yan-jing; Li, Ze-ming; Zhao, Dong-kang; Yu, Shui-ping.
Título: The application of Compont gel in chronic obstructive jaundice rats model
Fonte: Acta cir. bras;34(5):e201900504, 2019. tab, graf.
Idioma: en.
Projeto: Guangxi Medical and Health Appropriate Technology Research and Development Project; . Project Plan Document of Guangxi Key Laboratory Construction.
Resumo: Abstract Purpose: To establish a new rat model, the pathogenesis of which is closer to the clinical occurrence of chronic obstructive jaundice with liver fibrosis. Methods: 90 SD rats were randomly divided into 3 groups. Group A common bile duct ligation, group B common bile duct injection compont and group C injection saline. The serum of three groups was extracted, and the liver function was detected by ELISA. HE staining, Masson staining and immunohistochemistry were used to detect liver pathology. Results: Group B showed a fluctuant development of jaundice, obstructive degree reached a peak at 2 weeks, and decreased from 3 weeks. HA, LA and PCIII were significantly higher than control group. 3 weeks after surgery, liver tissue fibrosis occurred in group B, and a wide range of fiber spacing was formed at 5 weeks. Immunohistochemistry showed that hepatic stellate cells were more active than the control group. Conclusion: Intra-biliary injection of Compont gel is different from the classic obstructive jaundice animal model caused by classic bile duct ligation, which can provide an ideal rat model of chronic obstructive jaundice with liver fibrosis.
Descritores: Ductos Biliares/efeitos dos fármacos
Modelos Animais de Doenças
Géis/administração & dosagem
Cirrose Hepática/induzido quimicamente
-Aspartato Aminotransferases/sangue
Valores de Referência
Compostos Azo
Fatores de Tempo
Ductos Biliares/patologia
Bilirrubina/análise
Albumina Sérica/análise
Ensaio de Imunoadsorção Enzimática
Imuno-Histoquímica
Distribuição Aleatória
Reprodutibilidade dos Testes
Ratos Sprague-Dawley
Amarelo de Eosina-(YS)
Icterícia Obstrutiva/induzido quimicamente
Icterícia Obstrutiva/patologia
Fosfatase Alcalina/sangue
gama-Glutamiltransferase/sangue
Injeções
Cirrose Hepática/patologia
Verde de Metila
Limites: Animais
Feminino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  3 / 31 LILACS  
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Id: biblio-949896
Autor: Coelho, Polyana Galvão Bernardes; Souza, Maria Verônica de; Conceição, Lissandro Gonçalves; Viloria, Marlene Isabel Vargas; Bedoya, Sirley Adriana Ortiz.
Título: Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
Fonte: An. bras. dermatol;93(3):415-418, May-June 2018. graf.
Idioma: en.
Resumo: Abstract: The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the collagen fibers in the tissues. The authors intend to elucidate all steps to obtain and capture images of histological sections stained with picrosirius red and evaluated under polarized light microscopy, as well as possible artefacts that may occur.
Descritores: Pele/ultraestrutura
Coloração e Rotulagem/métodos
Compostos Azo/química
Colágeno/ultraestrutura
Microscopia de Polarização/métodos
-Pele/citologia
Birrefringência
Administração Cutânea
Fotomicrografia
Colágeno/análise
Colágenos Fibrilares/ultraestrutura
Cavalos
Limites: Animais
Cães
Responsável: BR1.1 - BIREME


  4 / 31 LILACS  
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Id: biblio-889238
Autor: Przystaś, Wioletta; Zabłocka-Godlewska, Ewa; Grabińska-Sota, Elżbieta.
Título: Efficiency of decolorization of different dyes using fungal biomass immobilized on different solid supports
Fonte: Braz. j. microbiol;49(2):285-295, Apr.-June 2018. tab, graf.
Idioma: en.
Projeto: Ministry of Science and Higher Education.
Resumo: Abstract Different technologies may be used for decolorization of wastewater containing dyes. Among them, biological processes are the most promising because they seem to be environmentally safe. The aim of this study was to determine the efficiency of decolorization of two dyes belonging to different classes (azo and triphenylmethane dyes) by immobilized biomass of strains of fungi (Pleurotus ostreatus - BWPH, Gleophyllum odoratum - DCa and Polyporus picipes - RWP17). Different solid supports were tested for biomass immobilization. The best growth of fungal strains was observed on the washer, brush, grid and sawdust supports. Based on the results of dye adsorption, the brush and the washer were selected for further study. These solid supports adsorbed dyes at a negligible level, while the sawdust adsorbed 82.5% of brilliant green and 19.1% of Evans blue. Immobilization of biomass improved dye removal. Almost complete decolorization of diazo dye Evans blue was reached after 24 h in samples of all strains immobilized on the washer. The process was slower when the brush was used for biomass immobilization. Comparable results were reached for brilliant green in samples with biomass of strains BWPH and RWP17. High decolorization effectiveness was reached in samples with dead fungal biomass. Intensive removal of the dyes by biomass immobilized on the washer corresponded to a significant decrease in phytotoxicity and a slight decrease in zootoxicity of the dye solutions. The best decolorization results as well as reduction in toxicity were observed for the strain P. picipes (RWP17).
Descritores: Basidiomycota/metabolismo
Poluentes Químicos da Água/metabolismo
Corantes/metabolismo
-Compostos Azo/metabolismo
Compostos de Tritil/metabolismo
Biotransformação
Células Imobilizadas/metabolismo
Adsorção
Águas Residuárias
Responsável: BR1.1 - BIREME


  5 / 31 LILACS  
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Id: biblio-1009153
Autor: Munjid Mustafa, Monawar; Jamal, Parveen; Fahmi Alkhatib, Ma'an; Senan Mahmod, Safa; Noraini Jimat, Dzun; Najhah Ilyas, Nurul.
Título: Panus tigrinus as a potential biomass source for Reactive Blue decolorization: isotherm and kinetic study
Fonte: Electron. j. biotechnol;26:7-11, Mar. 2017. tab, graf.
Idioma: en.
Resumo: Background: Textile and dye industries pose a serious threat to the environment. Conventional methods used for dye treatment are generally not always effective and environmentally friendly. This drove attention of scores of researchers to investigate alternative methods for the biodegradation of dyes using fungal strains. In this work, white-rot fungus (Panus tigrinus) was used as a biosorbent for the decolorization of Reactive Blue 19. The process parameters that were varied were initial concentration (50­150 mg/L), contact time (30­90 min), and pH (2­6). In addition, to gain important data for the evaluation of a sorption process, the equilibrium and kinetics of the process were determined. Results: White-rot fungus showed great potential in decolorizing Azo dyes. The strain showed the maximum decolorization of 83.18% at pH 2, a contact time of 90 min, and an initial concentration of 50 mg/L. The Langmuir isotherm described the uptake of the Reactive Blue 19 dye better than the Freundlich isotherm. Analysis of the kinetic data showed that the dye uptake process followed the pseudo second-order rate expression. Conclusion: The biosorption process provided vital information on the process parameters required to obtain the optimum level of dye removal. The isotherm study indicated the homogeneous distribution of active sites on the biomass surface, and the kinetic study suggested that chemisorption is the rate-limiting step that controlled the biosorption process. According to the obtained results, P. tigrinus biomass can be used effectively to decolorize textile dyes and tackle the pollution problems in the environment.
Descritores: Basidiomycota/química
Antraquinonas/química
Corantes/química
-Temperatura
Compostos Azo/química
Indústria Têxtil
Fatores de Tempo
Basidiomycota/metabolismo
Biodegradação Ambiental
Cinética
Adsorção
Isoterma
Concentração de Íons de Hidrogênio
Responsável: CL1.1 - Biblioteca Central


  6 / 31 LILACS  
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Texto completo SciELO Saúde Pública
Lefèvre, Fernando
Texto completo
Id: lil-733130
Autor: Caron, Eduardo; Lefèvre, Fernando; Lefèvre, Ana Maria Cavalcanti.
Título: In the final analysis, are we a consumer society or not? Implications for health / Afinal, somos ou não somos uma sociedade de consumo? Consequências para a saúde
Fonte: Ciênc. saúde coletiva;20(1):145-153, 01/2015.
Idioma: en.
Resumo: In this paper, the question of Brazil's insertion today as a country with the characteristics of modern consumer societies is discussed, focusing on the commercialization of the health sector, the segmentation of the health system and the contradictions of the rights to health care in the social context in question. Some research data on these issues broadcast in the National News Bulletins of Globo TV during the year of 2012 are presented, in which the high technology private hospital as a consumer icon, the underfunding of the public health system and the rejection of a poor and deprived Unified Health System are analyzed.

Discute-se aqui a nossa inserção como país, hoje, nas sociedades de consumo características da modernidade, enfocando a mercantilização na área da saúde, a segmentação do sistema de saúde e as contradições do direito à saúde no contexto social em questão. São apresentados dados de pesquisa sobre o tema no Jornal Nacional da Rede Globo de Televisão, durante o ano de 2012, na qual se analisa o hospital privado de alto padrão tecnológico como ícone de consumo, o subfinanciamento do sistema público de saúde e a rejeição de um Sistema Único de Saúde pobre e carente.
Descritores: Compostos Azo/análise
Cromatografia em Gel/métodos
Cromatografia Líquida de Alta Pressão/métodos
Espectrometria de Massas por Ionização por Electrospray/métodos
Especiarias/análise
Espectrometria de Massas em Tandem/métodos
-Compostos Azo/química
Capsicum/química
Análise de Alimentos/métodos
Corantes de Alimentos/análise
Corantes de Alimentos/química
Estrutura Molecular
Naftóis/análise
Naftóis/química
Reprodutibilidade dos Testes
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  7 / 31 LILACS  
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Id: biblio-966380
Autor: Alcântara, Tiago de Araújo Pinho; Oliveira, Juliana Mota; Barreto, Norma Suely Evangelista; Marbac, Phellippe Arthur Santos; Cazetta, Marcia Luciana.
Título: Aerobic decolorization of azo dye orange g by a new yeast isolate Candida cylindracea SJL6 / Descoloração aeróbica do azo corante alaranjado g por um novo isolado da levedura Candida cylindracea SJL6
Fonte: Biosci. j. (Online);33(5):1340-1350, sept./oct. 2017. ilus, graf.
Idioma: en.
Resumo: Biodecolorization of the azo dye Orange G was investigated using a new strain of Candida cylindracea SJL6, isolated from freshwater samples of the Subaé river in Bahia state, Brazil. Strain SJL6 was identified as C. cylindracea on the basis of 26S rDNA region. The various parameters of dye decolorization and cell growth were studied, including the Orange G dye concentration (100 to 500 ppm), temperature (20 to 40 °C), glucose concentration (0 to 5%), and initial pH (3 to 8). Biotoxicity tests were performed using shrimp (Artemia salina) to determine the lethal concentration (LC50) and onion bulbs (Allium cepa) to determine the cytotoxic and genotoxic effects of both Orange G dye and metabolites formed after decolorization. Up to 90% of decolorization of the Orange G dye at 500 ppm was achieved by C. cylindracea SJL6 at 30 °C, pH 3, and 1% glucose. However, the biotoxicity tests showed that there was increased toxicity after decolorization, suggesting partial Orange G dye degradation and production of toxic metabolites.

A biodescoloração do corante Alaranjado G foi investigada utilizando um novo isolado de Candida cylindracea SJL6, isolado de amostras de água do Rio Subaé, Bahia, Brasil. A linhagem SJL6 foi identificada como Candida cylindracea com base na região 26S do rDNA. Os parâmetros estudados na descoloração do corante e crescimento celular foram: concentração do Alaranjado G (100 a 500 ppm), temperatura (20 a 40 ºC), concentração de glicose (0 a 5%) e pH inicial (3 a 8). Os testes de biotoxicidade foram realizados utilizando o microcrustáceo Artemia salina para determinar a concentração letal (L50) e bulbos de cebola (Alium cepa) para determinar os efeitos citotóxicos e genotóxicos tanto do corante alaranjado G quanto dos metabólitos produzidos após a descoloração. Uma taxa de descoloração acima de 90% foi atingida a 500 ppm por C. cylindracea SJL6 a 30 ºC, pH 3 e 1% de glicose. Entretanto, os testes de biotoxicidade mostraram que ocorreu um aumento da toxicidade após a descoloração, o que sugere uma degradação parcial da molécula do corante Alaranjado G e produção de metabólitos tóxicos.
Descritores: Compostos Azo
Biodegradação Ambiental
Candida
Fungos
Responsável: BR396.1 - Biblioteca Central


  8 / 31 LILACS  
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Texto completo SciELO Chile
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Id: lil-734669
Autor: Salinas, Paulo; Figueroa Vergara, Saúl A; Bañados Fernández, Rodrigo.
Título: Estudio histoquímico de la distribución de las fibras de colágeno en hueso sesamoídeo distal de equinos con y sin síndrome navicular / Histochemical study of the distribution of collagen fibers on distal sesamoid bone in equine with and without navicular syndrome
Fonte: Int. j. morphol;32(4):1266-1270, Dec. 2014. ilus.
Idioma: es.
Resumo: La significancia funcional de cualquier relación que exista entre la orientación de las fibras de colágeno y la tensión ejercida sobre una estructura ósea dependerá de la influencia de estos parámetros microestructurales sobre las propiedades biomecánicas del hueso y su capacidad de adaptarse. Se estudió la localización del colágeno en el hueso sesamoídeo distal (HSD) de la mano en equinos con Síndrome Navicular para observar cómo este refleja las demandas biomecánicas ejercidas por la tensión que ejerce el tendón del músculo flexor digital profundo sobre la articulación interfalángica distal. Se utilizaron las tinciones hematoxilina-eosina-azul alcián, y Rojo Picrosirius de Junqueira. La birrefringencia de colágeno óseo fue determinada utilizando microscopía de luz polarizada. La remodelación del HSD resultó en la formación de osteonas secundarias transversales orientados en una dirección lateral a medial y el colágeno óseo se orientó de manera similar. Estos resultados proporcionan evidencia de la existencia de una relación entre la función mecánica de un hueso con su arquitectura, incluso demuestra que esta se extiende hasta el nivel molecular.

The functional significance of any relationship exists between the orientation of the collagen fibers and the strain on a bone structure depend on the influence of these microstructural parameters on the biomechanical properties of bone and its ability to adapt. Localization of collagen was studied in the distal sesamoid bone (DSB) in in equine foot with Navicular Syndrome to see how this reflects the biomechanical demands by the tension exerted by the tendon of the deep digital flexor muscle on the joint distal interphalangeal. Hematoxylin-eosin-alcian blue staining, and Red Picrosirius of Junqueira were used. The birefringence of bone collagen was determined using polarized light microscopy. The remodeling of DSB resulted in the formation of transverse secondary osteons oriented lateral to medial and bone collagen was oriented in similar direction. These results provide evidence for the existence of a relationship between the mechanical function of a bone with the architecture, and shows further that this extends up to the molecular level.
Descritores: Ossos Sesamoides/patologia
Colágeno/metabolismo
Doenças do Pé/veterinária
Doenças dos Cavalos
-Compostos Azo
Imuno-Histoquímica
Colágeno/ultraestrutura
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


  9 / 31 LILACS  
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Texto completo SciELO Brasil
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Id: lil-775119
Autor: Lalnunhlimi, Sylvine; Krishnaswamy, Veenagayathri.
Título: Decolorization of azo dyes (Direct Blue 151 and Direct Red 31) by moderately alkaliphilic bacterial consortium
Fonte: Braz. j. microbiol;47(1):39-46, Jan.-Mar. 2016. graf.
Idioma: en.
Resumo: Abstract Removal of synthetic dyes is one of the main challenges before releasing the wastes discharged by textile industries. Biodegradation of azo dyes by alkaliphilic bacterial consortium is one of the environmental-friendly methods used for the removal of dyes from textile effluents. Hence, this study presents isolation of a bacterial consortium from soil samples of saline environment and its use for the decolorization of azo dyes, Direct Blue 151 (DB 151) and Direct Red 31 (DR 31). The decolorization of azo dyes was studied at various concentrations (100–300 mg/L). The bacterial consortium, when subjected to an application of 200 mg/L of the dyes, decolorized DB 151 and DR 31 by 97.57% and 95.25% respectively, within 5 days. The growth of the bacterial consortium was optimized with pH, temperature, and carbon and nitrogen sources; and decolorization of azo dyes was analyzed. In this study, the decolorization efficiency of mixed dyes was improved with yeast extract and sucrose, which were used as nitrogen and carbon sources, respectively. Such an alkaliphilic bacterial consortium can be used in the removal of azo dyes from contaminated saline environment.
Descritores: Compostos Azo/metabolismo
Bactérias/metabolismo
Cor
Resíduos Industriais
Consórcios Microbianos
-Biotransformação
Bactérias/crescimento & desenvolvimento
Bactérias/isolamento & purificação
Carbono/metabolismo
Concentração de Íons de Hidrogênio
Nitrogênio/metabolismo
Microbiologia do Solo
Temperatura
Responsável: BR1.1 - BIREME


  10 / 31 LILACS  
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Texto completo SciELO Brasil
Texto completo
Id: lil-762909
Autor: Zotz, T.G.G.; Paula, J.B. de.
Título: Influence of transcutaneous electrical stimulation on heterotopic ossification: an experimental study in Wistar rats
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;48(11):1055-1062, Nov. 2015. tab, graf.
Idioma: en.
Resumo: Heterotopic ossification (HO) is a metaplastic biological process in which there is newly formed bone in soft tissues, resulting in joint mobility deficit and pain. Different treatment modalities have been tried to prevent HO development, but there is no consensus on a therapeutic approach. Since electrical stimulation is a widely used resource in physiotherapy practice to stimulate joint mobility, with analgesic and anti-inflammatory effects, its usefulness for HO treatment was investigated. We aimed to identify the influence of electrical stimulation on induced HO in Wistar rats. Thirty-six male rats (350-390 g) were used, and all animals were anesthetized for blood sampling before HO induction, to quantify the serum alkaline phosphatase. HO induction was performed by bone marrow implantation in both quadriceps of the animals, which were then divided into 3 groups: control (CG), transcutaneous electrical nerve stimulation (TENS) group (TG), and functional electrical stimulation (FES) group (FG) with 12 rats each. All animals were anesthetized and electrically stimulated twice per week, for 35 days from induction day. After this period, another blood sample was collected and quadriceps muscles were bilaterally removed for histological and calcium analysis and the rats were killed. Calcium levels in muscles showed significantly lower results when comparing TG and FG (P<0.001) and between TG and CG (P<0.001). Qualitative histological analyses confirmed 100% HO in FG and CG, while in TG the HO was detected in 54.5% of the animals. The effects of the muscle contractions caused by FES increased HO, while anti-inflammatory effects of TENS reduced HO.
Descritores: Ossificação Heterotópica/terapia
Músculo Quadríceps
Estimulação Elétrica Nervosa Transcutânea
-Anti-Inflamatórios
Compostos Azo
Fosfatase Alcalina/sangue
Transplante de Medula Óssea
Estudos Transversais
Cálcio/análise
Modelos Animais de Doenças
Terapia por Estimulação Elétrica
Amarelo de Eosina-(YS)
Verde de Metila
Ossificação Heterotópica/etiologia
Ossificação Heterotópica/patologia
Músculo Quadríceps/química
Músculo Quadríceps/patologia
Distribuição Aleatória
Ratos Wistar
Transplante Autólogo
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME



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