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Id: biblio-838060
Autor: Torii, Kazuhiro; Maeshige, Noriaki; Aoyama-Ishikawa, Michiko; Miyoshi, Makoto; Terashi, Hiroto; Usami, Makoto.
Título: Combination therapy with butyrate and docosahexaenoic acid for keloid fibrogenesis: an in vitro study
Fonte: An. bras. dermatol;92(2):184-190, Mar.-Apr. 2017. tab, graf.
Idioma: en.
Projeto: JSPS KAKENHI.
Resumo: Abstract: Background: A single, effective therapeutic regimen for keloids has not been established yet, and the development of novel therapeutic approaches is expected. Butyrate, a short-chain fatty acid, and docosahexaenoic acid (DHA), a ω-3 polyunsaturated fatty acid, play multiple anti-inflammatory and anticancer roles via their respective mechanisms of action. Objective: In this study, we evaluated the antifibrogenic effects of their single and combined use on keloid fibroblasts. Methods: Keloid fibroblasts were treated with butyrate (0-16 mM) and/or DHA (0-100 µM) for 48 or 96 h. Results: Butyrate inhibited cell proliferation, and α-smooth muscle actin (α-SMA) and type III collagen expressions, with inhibition of the transforming growth factor (TGF)-β1 and TGF-β type I receptor expressions and increased prostaglandin E2 with upregulation of cyclooxygenase-1 expression with induction of histone acetylation. DHA inhibited α-SMA, type III collagen, and TGF-β type I receptor expressions. Then, the butyrate/DHA combination augmented the antifibrogenic effects, resulting in additional inhibition of α-SMA, type I and III collagen expressions, with strong disruption of stress fiber and apoptosis induction. Moreover, the butyrate/DHA combination inhibited the cyclooxygenase-2 expression, suggesting stronger anti-inflammatory effect than each monotherapy. Study limitations: Activation in keloid tissue is affected not only by fibroblasts but also by epithelial cells and immune cells. Evaluation of the effects by butyrate and DHA in these cells or in an in vivo study is required. Conclusion: This study demonstrated that butyrate and docosahexaenoic acid have antifibrogenic effects on keloid fibroblasts and that these may exert therapeutic effects for keloid.
Descritores: Butiratos/uso terapêutico
Ácidos Docosa-Hexaenoicos/uso terapêutico
Fibroblastos
Queloide/tratamento farmacológico
-Células Cultivadas
Proteínas Serina-Treonina Quinases
Receptores de Fatores de Crescimento Transformadores beta
Terapia Combinada
Colágeno Tipo I
Colágeno Tipo III
Proliferação de Células
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1017382
Autor: Mamimin, Chonticha; Prasertsan, Poonsuk; Kongjan, Prawit; O-Thong, Sompong.
Título: Effects of volatile fatty acids in biohydrogen effluent on biohythane production from palm oil mill effluent under thermophilic condition
Fonte: Electron. j. biotechnol;29:78-85, sept. 2017. tab, graf, ilus.
Idioma: en.
Projeto: Khon Kaen University; . Thailand Research Fund.
Resumo: Background: Biohydrogen effluent contains a high concentration of volatile fatty acid (VFA) mainly as butyric, acetic, lactic and propionic acids. The presence of various VFAs (mixture VFAs) and their cooperative effects on two-stage biohythane production need to be further studied. The effect of VFA concentrations in biohydrogen effluent of palm oil mill effluent (POME) on methane yield in methane stage of biohythane production was investigated. Results: The methane yield obtained in low VFA loading (0.9 and 1.8 g/L) was 15­20% times greater than that of high VFA loading (3.6 and 4.7 g/L). Butyric acid at high concentrations (8 g/L) has the individual significantly negative effect the methane production process (P b 0.05). Lactic, acetic and butyric acid mixed with propionic acid at a concentration higher than 0.5 g/L has an interaction significantly negative effect on the methanogenesis process (P b 0.05). Inhibition condition had a negative effect on both bacteria and archaea with inhibited on Geobacillus sp., Thermoanaerobacterium thermosaccharolyticum, Methanoculleus thermophilus and Methanothermobacter delfuvii resulting in low methane yield. Conclusion: Preventing the high concentration of butyric acid, and propionic acid in the hydrogenic effluent could enhance methane production in two-stage anaerobic digestion for biohythane production.
Descritores: Propionatos/metabolismo
Butiratos/metabolismo
Águas Residuárias/microbiologia
Metano/biossíntese
-Propionatos/análise
Butiratos/análise
Óleo de Palmeira
Methanobacteriaceae
Archaea
Methanomicrobiaceae
Geobacillus
Fermentação
Águas Residuárias/análise
Hidrogênio
Anaerobiose
Responsável: CL1.1 - Biblioteca Central


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Id: lil-18006
Autor: Ruiz Martinez, H; Jaramillo Uricoechea, R.
Título: Tratamiento medico de las cervicitis con un polimero del acido gamma amino butirico / Clinical treatment of cervicitis with a polymer of the gamma amino butyric acid
Fonte: Rev. colomb. obstet. ginecol;34(2):115-120, 1983.
Idioma: pt.
Descritores: Butiratos
Cervicite Uterina
Polímeros
Responsável: BR1.1 - BIREME


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Id: biblio-885130
Autor: Mendez, Ernesto Vargas.
Título: Caracterização do efeito anti-neoplásico do butirato em duas linhagens celulares de rato derivadas de tumores hepáticos com diferente ní­vel de diferenciação / Characterization of the anti-neoplastic effect of butyrate in two mouse cell lines derived from hepatic tumors with different levels of differentiation.
Fonte: São Paulo; s.n; 2018. 75 p. tab, ilus, graf.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Ciências Farmacêuticas para obtenção do grau de Doutor.
Resumo: O carcinoma hepatocelular (HCC) é uma neoplasia primária com mau prognóstico e alta taxa de recorrência. Estudos recentes demostram que o HCC pode ser classificado em três subtipos segundo o perfil molecular. Destes subtipos, o HCC pouco diferenciado apresenta pior prognostico. Neste sentido, torna-se de particular interesse o estudo de compostos com efeitos diferenciadores e citotóxicos nas células destas neoplasias pouco diferenciadas. O butirato, um ácido graxo de cadeia curta produzido pela fermentação microbiana da fibra alimentar no intestino, tem demonstrado atividade anti-neoplásica e capacidade moduladora da diferenciação celular em diversos tipos celulares, incluindo linhagens de HCC humano e células progenitoras hepáticas. Assim, objetivou-se neste estudo, caracterizar o efeito do butirato de sódio (NaBu) em duas linhagens de células neoplásicas de rato: uma pouco diferenciada (GP7TB) e a outra, uma linhagem derivada de um HCC diferenciado (JM-1). A linhagem GP7TB mostrou maior resistência ao NaBu (ED50= 7,7 mM) do que as células JM-1 (ED50= 5,2 mM). A redução na viabilidade celular após 72 h de tratamento com NaBu esteve relacionada com a diminuição na proliferação celular e no caso das células GP7TB, de um aumento na apoptose. O tratamento com NaBu induziu alterações morfológicas nas duas linhagens celulares, porém apenas nas células do tipo GP7TB, essas alterações sugerem um processo de diferenciação/transdiferenciação celular. O aumento na expressão de genes envolvidos no controle da pluripotência de células tronco, assim como de alguns marcadores de células tronco, sugere que o NaBu induziu uma reprogramação profunda das células GP7TB. Por outro lado, a redução na expressão de genes relacionados com migração e plasticidade celular assim como de proliferação celular apontam que estas células diminuíram seu potencial invasivo e a capacidade de autorenovação. Embora sejam necessárias análises adicionais para confirmar o efeito observado nos perfis de expressão gênica, os resultados deste estudo sugerem que o NaBu apresenta efeito antineoplásico por meio da redução da proliferação, aumento da apoptose e modulação da expressão de genes associados com a transição epitéliomesenquimal em células com características tronco tumorais

Hepatocellular carcinoma (HCC) is a primary neoplasia with poor prognosis and high recurrence rate. Recent evidence suggests that HCC can be classified in three different subtypes based on their molecular profile. Among these subtypes, the poorlydifferentiated HCC has the worst prognosis. Therefore, the study of compounds with pro-differentiating and cytotoxic effects on poorly-differentiated neoplastic cells represents a matter of primary concern. Butyrate which is a short-chain fatty acid produced by microbial fermentation in the intestine, has demonstrated anti-neoplastic activity and pro-differentiating potential in several cell types, including, human HCC cell lines and liver progenitor cells. In this study, we aimed to characterize the effect of sodium butyrate (NaBu) on two neoplastic cell lines derived from rats: a poorlydifferentiated cell line (GP7TB) and, a cell line derived from a well-differentiated HCC. GP7TB showed increased resistance to NaBu treatment (ED50= 7.7 mM) compared to JM-1 (ED50= 5.2 mM). The reduction in cell viability observed after 72 h of treatment was explained by a reduction in cell proliferation and, in the case of GP7TB, by increased levels of apoptosis. The NaBu treatment induced morphological alterations in both cell lines. However, only in the case of GP7TB cells, the alterations suggested a differentiation/transdifferentiation process. The up-regulation of genes involved in pluripotency and genes expressing stem cell markers indicated that NaBu triggered a deep reprogramming of GP7TB cells. Besides, a down-regulation in the expression of genes related with cell migration and plasticity suggested that these cells reduced their invasive potential and their self-renewal capacity. Additional analyses are necessary to confirm the observed effect on gene expression profiles. However, the results of this study suggest that NaBu exert anti-neoplastic effects through apoptosis, reduction of cell proliferation and downregulation of genes associated with epithelial-mesenchymal transition of cancer stem-like cells
Descritores: Antineoplásicos/efeitos adversos
Butiratos/análise
Carcinoma Hepatocelular/prevenção & controle
-Ácido Butírico
Linhagem Celular
Interpretação Estatística de Dados
Citometria de Fluxo/métodos
Imunofenotipagem/instrumentação
Células-Tronco Neoplásicas
Análise Estatística
Limites: Animais
Ratos
Tipo de Publ: Revisão
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas
BR40.1; T 616.99436, V297c. 30100022439-F


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Id: lil-748258
Autor: Zafar-ul-Hye, Muhammad; Farooq, Hafiz Muhammad; Hussain, Mubshar.
Título: Bacteria in combination with fertilizers promote root and shoot growth of maize in saline-sodic soil
Fonte: Braz. j. microbiol;46(1):97-102, 05/2015. tab.
Idioma: en.
Resumo: Salinity is the leading abiotic stress hampering maize (Zea mays L.) growth throughout the world, especially in Pakistan. During salinity stress, the endogenous ethylene level in plants increases, which retards proper root growth and consequent shoot growth of the plants. However, certain bacteria contain the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, which converts 1-aminocyclopropane-1-carboxylic acid (an immediate precursor of ethylene biosynthesis in higher plants) into ammonia and α-ketobutyrate instead of ethylene. In the present study, two Pseudomonas bacterial strains containing ACC-deaminase were tested separately and in combinations with mineral fertilizers to determine their potential to minimize/undo the effects of salinity on maize plants grown under saline-sodic field conditions. The data recorded at 30, 50 and 70 days after sowing revealed that both the Pseudomonas bacterial strains improved root and shoot length, root and shoot fresh weight, and root and shoot dry weight up to 34, 43, 35, 71, 55 and 68%, respectively, when applied without chemical fertilizers: these parameter were enhanced up to 108, 95, 100, 131, 100 and 198%, respectively, when the strains were applied along with chemical fertilizers. It can be concluded that ACC-deaminase Pseudomonas bacterial strains applied alone and in conjunction with mineral fertilizers improved the root and shoot growth of maize seedlings grown in saline-sodic soil.
Descritores: Desenvolvimento Vegetal
Raízes de Plantas/fisiologia
Brotos de Planta/fisiologia
Pseudomonas/crescimento & desenvolvimento
Microbiologia do Solo
Solo/química
Zea mays/fisiologia
-Aminoácidos Cíclicos/metabolismo
Amônia/metabolismo
Butiratos
Carbono-Carbono Liases/metabolismo
Fertilizantes
Paquistão
Pseudomonas/enzimologia
Salinidade
Responsável: BR1.1 - BIREME


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Id: lil-732517
Autor: Feltrin, Rebeca Buzzo; Velho, Lea Maria Leme Strini.
Título: Entre o campo e o laboratório: a dinâmica de produção de conhecimento no Ambulatório de Menopausa do Caism/Unicamp / Between the country and the laboratory: the dynamics of the production of knowledge at the Menopause Outpatients Clinic at Caism, Unicamp
Fonte: Hist. ciênc. saúde-Manguinhos;21(4):1283-1300, Oct-Dec/2014.
Idioma: pt.
Resumo: Este estudo investiga as práticas de produção de conhecimento sobre a menopausa no Caism/Unicamp, centro de referência para políticas públicas em saúde da mulher. Foram realizadas observações de consultas ginecológicas, entrevistas com mulheres e médicos e observação de reuniões de apoio psicológico, buscando identificar os discursos que circulam no lugar e o processo de alistamento de diferentes atores para que os conhecimentos ali produzidos alcancem credibilidade e “viajem” além dos limites do hospital-escola, tornando-se “universais”. A análise baseia-se nos “estudos localistas”, alinhados aos estudos sociais de ciência e tecnologia.

This study investigates the practices involved in the production of knowledge about menopause at Caism, Unicamp, a reference center for public policies for women’s health. Gynecological appointments and psychological support meetings were observed, and women and doctors were interviewed in order to identify what discourse circulates there and how different actors are brought in to ensure that the knowledge produced attains credibility and “travels” beyond the boundaries of the teaching hospital to become “universal”. The analysis is based on localized studies aligned with social studies of science and technology.
Descritores: /genética
H-TEMEFOS ANTIGENS/genética
Complexo Principal de Histocompatibilidade
Odorantes
-Ácido Benzoico
Benzoatos/isolamento & purificação
Benzoatos/urina
Butiratos/isolamento & purificação
Butiratos/urina
Cromatografia Gasosa
Cromatografia por Troca Iônica
Cresóis/isolamento & purificação
Cresóis/urina
Dimetil Sulfóxido
Discriminação Psicológica
Aprendizagem em Labirinto
Camundongos Endogâmicos
Fenóis/isolamento & purificação
Fenóis/urina
Fenilacetatos/isolamento & purificação
Fenilacetatos/urina
Sulfonas/isolamento & purificação
Sulfonas/urina
Ultrafiltração
Limites: Animais
Masculino
Camundongos
Tipo de Publ: Research Support, U.S. Gov't, P.H.S.
Responsável: BR1.1 - BIREME


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Id: lil-699782
Autor: Lisboa, Helen Cristina Fávero; Biasetto, Carolina Rabal; Medeiros, João Batista de; Araújo, Ângela Regina; Silva, Dulce Helena Siqueira; Teles, Helder Lopes; Trevisan, Henrique Celso.
Título: Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator
Fonte: Braz. j. microbiol;44(3):923-926, July-Sept. 2013. ilus, tab.
Idioma: en.
Resumo: A sensitive and efficient colorimetric method was optimized for detection of esterase enzymes produced by endophytic fungi for development of High-Throughput Screening (HTS). The fungi were isolated and obtained previously from plant species of Cerrado and Atlantic Forest located in areas of environmental preservation in the State of Sao Paulo / Brazil, as part of the project "Chemical and biological prospecting endophytic fungi associated to plant species of Cerrado and Atlantic Forest". The compounds ethyl butyrate, ethyl acetate and methyl propionate were used as standards esters which were hydrolyzed by extracellular enzyme from endophytic fungi (EC. 3.1.1.1 -carboxylesterases) for production of carboxylic acids. Thus, the reduction of the pH increases the protonated indicator concentration (bromothymol blue), changing the color of the reaction medium (from blue to yellow), that can be observed and measured by spectrophotometry at 616 nm. The methodology with acid-base indicator was performed on 13 microorganisms, aiming Periconia atropurpurea asapotential source of esterase for biotransformation of short chain esters. The results also evidenced that this methodology showed to be efficient, fast, cheap, having low consumption of reagents and easy development, and can be applied to screen carboxylic-ester hydrolases in a large number of microorganisms.
Descritores: Colorimetria/métodos
Endófitos/enzimologia
Esterases/análise
Fungos/enzimologia
-Acetatos/metabolismo
Brasil
Butiratos/metabolismo
Fungos/isolamento & purificação
Concentração de Íons de Hidrogênio
Indicadores e Reagentes
Plantas/microbiologia
Propionatos/metabolismo
Tipo de Publ: Estudo de Avaliação
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-672048
Autor: Berlemont, Renaud; Spee, Olivier; Delsaute, Maud; Lara, Yannick; Schuldes, Jörg; Simon, Carola; Power, Pablo; Daniel, Rolf; Galleni, Moreno.
Título: Novel organic solvent-tolerant esterase isolated by metagenomics: insights into the lipase/esterase classification / Nueva esterasa tolerante a los solventes orgánicos aislada por metagenómica: ideas sobre la clasificación de las esterasas/lipasas
Fonte: Rev. argent. microbiol;45(1):3-12, mar. 2013. graf, tab.
Idioma: en.
Resumo: In order to isolate novel organic solvent-tolerant (OST) lipases, a metagenomic library was built using DNA derived from a temperate forest soil sample. A two-step activity-based screening allowed the isolation of a lipolytic clone active in the presence of organic solvents. Sequencing of the plasmid pRBest recovered from the positive clone revealed the presence of a putative lipase/esterase encoding gene. The deduced amino acid sequence (RBest1) contains the conserved lipolytic enzyme signature and is related to the previously described OST lipase from Lysinibacillus sphaericus 205y, which is the sole studied prokaryotic enzyme belonging to the 4.4 a/ß hydrolase subgroup (abH04.04). Both in vivo and in vitro studies of the substrate specificity of RBest1, using triacylglycerols or nitrophenyl-esters, respectively, revealed that the enzyme is highly specific for butyrate (C4) compounds, behaving as an esterase rather than a lipase. The RBest1 esterase was purified and biochemically characterized. The optimal esterase activity was observed at pH 6.5 and at temperatures ranging from 38 to 45 °C. Enzymatic activity, determined by hydrolysis of p-nitrophenyl esters, was found to be affected by the presence of different miscible and non-miscible organic solvents, and salts. Noteworthy, RBest1 remains significantly active at high ionic strength. These findings suggest that RBest1 possesses the ability of OST enzymes to molecular adaptation in the presence of organic compounds and resistance of halophilic proteins.

Con el fin de aislar nuevas variantes de lipasas tolerantes a solventes organicos (OST), se construyo una libreria metagenomica a partir de ADN obtenido de una muestra de suelo de bosque templado. A traves de un monitoreo en dos etapas, basado en la deteccion de actividades, se aislo un clon con actividad lipolitica en presencia de solventes organicos. La secuenciacion del plasmido pRBest recuperado del clon positivo revelo la presencia de un gen codificante de una hipotetica lipasa/esterasa. La secuencia deducida de amino acidos (RBest1) contiene los motivos conservados de enzimas lipoliticas y esta relacionada con la lipasa OST previamente descrita de Lysinibacillus sphaericus 205y, que es la unica enzima procariota estudiada perteneciente al subgrupo 4.4 de a/ß hidrolasas (abH4.04). Estudios in vivo e in vitro sobre la especificidad de sustratos de RBest1, utilizando triacil-gliceroles o p-nitrofenil-esteres, respectivamente, revelaron que la enzima es altamente especifica para compuestos butiricos (C4), comportandose como una esterasa y no como una lipasa. La esterasa RBest1 fue purificada y caracterizada bioquimicamente. La actividad optima de esterasa fue observada a pH 6,5 y las temperaturas optimas fueron entre 38 y 45 °C. Se establecio que la actividad enzimatica, determinada por hidrolisis de p-nitrofenil esteres, es afectada en presencia de diferentes solventes organicos miscibles y no miscibles, y tambien sales. Notoriamente, RBest1 permanece significativamente activa a elevadas fuerzas ionicas. Estos hallazgos sugieren que RBest1 posee la capacidad de las enzimas OST de la adaptacion molecular en presencia de compuestos organicos, asi como la resistencia de las proteinas halofilas.
Descritores: Esterases/isolamento & purificação
Lipase/isolamento & purificação
Metagenômica
-Sequência de Aminoácidos
Bacillaceae/enzimologia
Proteínas de Bactérias/química
Butiratos/metabolismo
Sequência Conservada
DNA
Esterases/classificação
Alemanha
Concentração de Íons de Hidrogênio
Hidrólise
Lipólise
Lipase/classificação
Dados de Sequência Molecular
Concentração Osmolar
Filogenia
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Microbiologia do Solo
Especificidade por Substrato
Sais/farmacologia
Solventes/farmacologia
Temperatura
Árvores
Triglicerídeos/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Id: lil-646329
Autor: Andrade, F.O.; Nagamine, M.K.; Conti, A. De; Chaible, L.M.; Fontelles, C.C.; Jordão Junior, A.A.; Vannucchi, H.; Dagli, M.L.Z.; Bassoli, B.K.; Moreno, F.S.; Ong, T.P..
Título: Efficacy of the dietary histone deacetylase inhibitor butyrate alone or in combination with vitamin A against proliferation of MCF-7 human breast cancer cells
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;45(9):841-850, Sept. 2012. ilus.
Idioma: en.
Resumo: The combined treatment with histone deacetylase inhibitors (HDACi) and retinoids has been suggested as a potential epigenetic strategy for the control of cancer. In the present study, we investigated the effects of treatment with butyrate, a dietary HDACi, combined with vitamin A on MCF-7 human breast cancer cells. Cell proliferation was evaluated by the crystal violet staining method. MCF-7 cells were plated at 5 x 10(4) cells/mL and treated with butyrate (1 mM) alone or combined with vitamin A (10 µM) for 24 to 120 h. Cell proliferation inhibition was 34, 10 and 46% following treatment with butyrate, vitamin A and their combination, respectively, suggesting that vitamin A potentiated the inhibitory activities of butyrate. Furthermore, exposure to this short-chain fatty acid increased the level of histone H3K9 acetylation by 9.5-fold (Western blot), but not of H4K16, and increased the expression levels of p21WAF1 by 2.7-fold (Western blot) and of RARβ by 2.0-fold (quantitative real-time PCR). Our data show that RARβ may represent a molecular target for butyrate in breast cancer cells. Due to its effectiveness as a dietary HDACi, butyrate should be considered for use in combinatorial strategies with more active retinoids, especially in breast cancers in which RARβ is epigenetically altered.
Descritores: Anticarcinógenos/farmacologia
Neoplasias da Mama/patologia
Butiratos/farmacologia
Proliferação de Células/efeitos dos fármacos
Metilação de DNA/efeitos dos fármacos
Inibidores de Histona Desacetilases/farmacologia
Vitamina A/farmacologia
-Anticarcinógenos/administração & dosagem
Butiratos/administração & dosagem
Inibidores de Histona Desacetilases/administração & dosagem
MCF-ABDOMINAL INJURIES CELLS
Vitamina A/administração & dosagem
Limites: Feminino
Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-599913
Autor: Lameiro, Thais Miguel do Monte; Silva, Camila Morais Gonçalves da; Marques, Letícia Helena Sousa; Cunha, Fernando Lorenzetti da; Almeida, Marcos Gonçalves de; Pereira, José Aires; Martinez, Carlos Augusto Real.
Título: Efeitos do butirato nos níveis de peroxidação lipídica em células da mucosa cólica sem trânsito fecal: estudo experimental em ratos / Effects of butyrate on levels of lipid peroxidation in cells of the colonic mucosa without fecal stream: experimental study in rats
Fonte: Rev. bras. colo-proctol;31(2):155-164, abr.-jun. 2011. ilus, tab.
Idioma: pt.
Resumo: Os ácidos graxos de cadeia curta (AGCC) representam o principal substrato energético para células da mucosa cólica. A derivação intestinal, reduzindo suprimento de AGCC, responsabiliza-se pela colite de exclusão (CE). Aplicação retal de butirato tem sido eficaz no tratamento da doença. Então, o objetivo deste estudo foi avaliar os níveis de lipoperoxidação na mucosa cólica, após aplicação de butirato, em modelo de CE. Vinte seis ratos Wistar foram submetidos à colostomia proximal e fístula mucosa distal. Os animais foram divididos em dois grupos segundo sacrifício ser realizado em duas ou quatro semanas. Cada grupo foi subdividido em dois subgrups segundo intervenção com soro fisiológico ou butirato. O diagnóstico de CE foi estabelecido por estudo histopatológico e os níveis de lipoperoxidação pelos níveis de malondialdeído (MDA). Utilizaram-se os testes de Mann-Whitney e Kruskal-Wallis (significantes quando p<0,05). Após duas semanas, os níveis de MDA foram menores nos segmentos sem trânsito nos animais irrigados com butirato (p=0,006); porém, após quatro semanas foram semelhantes (p=0,08). No cólon sem trânsito irrigado com butirato, os níveis de MDA aumentaram com o tempo de exclusão (p=0,02); enquanto no cólon com trânsito não se modificaram (p=0,86). O butirato reduz os níveis de MDA na mucosa cólica sem trânsito fecal, após duas semanas de derivação; entretanto, a irrigação isolada não é capaz de reduzir os níveis de lipoperoxidação das células mucosas com o progredir do tempo de exclusão intestinal.

The short-chain fatty acids (SCFA) are the main energy substrate for the cells of the colonic mucosa. Diversion of the fecal stream reducing the supply of SCFA is responsible for diversion colitis (DC). Rectal application of butyrate has been demonstrated effective in the treatment of the disease. So the aim of this study was to evaluate the levels of lipid peroxidation in the colon mucosa after application of butyrate in model of DC. Twenty-six rats were submitted to proximal colostomy and distal mucous fistula. The animals were divided into two groups according sacrifice carried out in two or four weeks. Each group was divided into two subgroups according to intervention with saline solution or butyrate. The diagnosis of colitis was established by histopathology and the levels of lipid peroxidation by tissue levels of malondialdehyde (MDA). We used the Mann-Whitney and Kruskal-Wallis, establishing a significance level of 5 percent (significant with p<0.05). After two weeks, the levels of MDA were lower in the segments without fecal stream of animals irrigated with butyrate (p=0.006), but after four weeks were similar (p=0.08). In the colon without fecal stream irrigated with butyrate, MDA levels increased with the time (p=0.02), while in segments with fecal stream MDA not changed. (p=0.86). Butyrate reduces the levels of MDA in the colonic mucosa without fecal stream, after two weeks of derivation. However, the irrigation with the substance is not able to reduce the lipid peroxidation of mucosal cells with increasing time of intestinal exclusion.
Descritores: Butiratos
Colite/diagnóstico
Ácidos Graxos Voláteis
Peroxidação de Lipídeos
Estresse Oxidativo
-Malondialdeído
Modelos Animais
Ratos Wistar
Limites: Animais
Ratos
Responsável: BR15.1 - Biblioteca de Ciências Biomédicas



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