Base de dados : LILACS
Pesquisa : D02.241.081.337.351.502 [Categoria DeCS]
Referências encontradas : 5 [refinar]
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Id: lil-659653
Autor: Sotomaior, P.; Araújo, L.M.; Nishikawa, C.Y.; Huergo, L.F.; Monteiro, R.A.; Pedrosa, F.O.; Chubatsu, L.S.; Souza, E.M..
Título: Effect of ATP and 2-oxoglutarate on the in vitro interaction between the NifA GAF domain and the GlnB protein of Azospirillum brasilense
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;45(12):1135-1140, Dec. 2012. ilus, tab.
Idioma: en.
Resumo: Azospirillum brasilense is a diazotroph that associates with important agricultural crops and thus has potential to be a nitrogen biofertilizer. The A. brasilense transcription regulator NifA, which seems to be constitutively expressed, activates the transcription of nitrogen fixation genes. It has been suggested that the nitrogen status-signaling protein GlnB regulates NifA activity by direct interaction with the NifA N-terminal GAF domain, preventing the inhibitory effect of this domain under conditions of nitrogen fixation. In the present study, we show that an N-terminal truncated form of NifA no longer required GlnB for activity and lost regulation by ammonium. On the other hand, in trans co-expression of the N-terminal GAF domain inhibited the N-truncated protein in response to fixed nitrogen levels. We also used pull-down assays to show in vitro interaction between the purified N-terminal GAF domain of NifA and the GlnB protein. The results showed that A. brasilense GlnB interacts directly with the NifA N-terminal domain and this interaction is dependent on the presence of ATP and 2-oxoglutarate.
Descritores: Trifosfato de Adenosina/metabolismo
Azospirillum brasilense/enzimologia
Proteínas de Bactérias/metabolismo
Ácidos Cetoglutáricos/metabolismo
Fatores de Transcrição/metabolismo
beta-Galactosidase/metabolismo
-Azospirillum brasilense/metabolismo
Vetores Genéticos
Plasmídeos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-552033
Autor: Bergmeyer, H. U; Horder, M; Rej, R.
Título: Recomendação aprovada (1985) para métodos da IFCC de medida de concentração catalítica de enzimas - parte 2 método IFCC para aspartato aminotransferase / Recommendation approved (1985) IFCC methods for measurement of catalytic concentration of enzymes - Part 2 IFCC method for aspartate aminotransferase
Fonte: Rev. bras. anal. clin;21(2):51-62, 1989. tab, graf.
Idioma: pt.
Descritores: Alanina Transaminase
Fosfatase Alcalina
Aspartato Aminotransferases
Creatina Quinase
Enzimas
Ácidos Cetoglutáricos
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


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Id: lil-217039
Autor: Giuffrida de M., Maria; Hernández de P., Illeana; Chacín-Melean, Jesús.
Título: Papel del calcio en la regulacion de la oxidacion del oxoglutarato en la mucosa gastrica del conejo / Role of calcium in regulation of oxoglutarate oxidation in the rabbit gastric mucosa
Fonte: Acta cient. venez;47(4):231-7, 1996. tab, graf.
Idioma: es.
Resumo: The stimulation of oxyntic cell by gastric secretagogues is associated to the activation of oxidative metabolism. The mechanisms of this activation are not exactly known. In the present work, we investigated the possible direct effect of Ca2+ on both oxoglutarate oxidation in rabbit gastric glands and oxoglutarate dehydrogenase activity (OGDH) in isolated gastric mitochondria. Both carbachol and Ca2+ ionophores (A23187 and ionomycin) significantly stimulated oxoglutarate oxidation in a dose-dependent manner. This effect was only observed in the presence of low substrate concentrations. APTA-AM, an intracellular calcium chelator, significantly inhibited the rate of oxoglutarate oxidation. OGDH activity was stimulated by physiological concentrations of Ca2+ in a dose-dependent manner. This effect was reduced by ruthenium red, an inhibitor of mitochondrial Ca2+ uptake, and it was additionally increased by spermine, a stimulant of Ca2+ influx to the mitochondria. The results support the hypothesis that Ca2+ plays a direct role in the activation of oxidative metabolism in the oxyntic cell
Descritores: Ácidos Cetoglutáricos/metabolismo
Cálcio/fisiologia
Mucosa Gástrica/metabolismo
Oxirredutases/metabolismo
-Carbacol/farmacologia
Células Parietais Gástricas
Células Parietais Gástricas/metabolismo
Ionóforos/farmacologia
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Mucosa Gástrica
Limites: Animais
Coelhos
Responsável: BR1.1 - BIREME


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Id: lil-217032
Autor: Giuffrida de M., Maria; Hernández P., Ileana; Chacin-Melean, Jesus.
Título: Papel del calcio en la regulacion de la oxidacion del oxoglutarato en la mucosa gastrica del conejo / Role of calcium in regulation of oxoglutarate oxidation in the rabbit gastric mucosa
Fonte: Acta cient. venez;47(1):5-10, 1996. tab, graf.
Idioma: es.
Resumo: The stimulation of the oxyntic cell by gastric secretagogues is associated to the activation of oxidative metabolism. The mechanisms of this activation are not exactly known. In the present work, we investigated the possible direct effect of Ca2+ on both oxoglutarate oxidation in rabbit gastric glands and oxoglutarate dehydrogenase activity (OGDH) is isolated gastric mitochondria. Both carbachol and Ca2+ ionophores +(A23187 and ionomycin) significantly stimulated oxoglutarate oxidation in a dose-dependent manner. This effect was only observed in the presence of low substrate concentrations. BAPTA-AM, an intracellular calcium chelator, significantly inhibited the rate of oxoglutarate oxidation. OGDH activity was stimulated by physiological concentrations of Ca2+ in a dose-depentent manner. This effect was reduced by ruthenium red, an inhibitor of mitochondrial Ca2+ uptake, and it was additionally increased by spermine, a stimulant of Ca2+ influx to the mitochondria. Results support the hypothesis that Ca2+ plays a direct role in the activation of oxidative metabolism in the oxyntic cell
Descritores: Ácidos Cetoglutáricos/metabolismo
Cálcio/fisiologia
Mucosa Gástrica/metabolismo
Oxirredutases/metabolismo
-Carbacol/farmacologia
Células Parietais Gástricas
Células Parietais Gástricas/metabolismo
Ionóforos/farmacologia
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Mucosa Gástrica
Mucosa Gástrica/enzimologia
Limites: Animais
Coelhos
Responsável: BR1.1 - BIREME


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Id: lil-10784
Autor: Rosasca, S. A; Damato, M. C; Bettinotti, S; Giuseppucci, J; Steinberg, P.
Título: Fisura labiopalatina: comportamiento del acido alfacetoglutarico y de los aminoacidos en sangre y orina. Modelo clinico experimental. / Cleft palate: behavior of the alpha-ketoglutaric acid and the aminoacids in blood and urine. Experimental clinical model
Fonte: Arch. argent. pediatr;80(5/6):548-54, 1982.
Idioma: es.
Descritores: Aminoácidos
Fissura Palatina
Ácidos Cetoglutáricos
Limites: Lactente
Seres Humanos
Responsável: BR1.1 - BIREME



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