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Pesquisa : D02.241.081.337.463 [Categoria DeCS]
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Id: biblio-1051447
Autor: Brizuela, Natalia; Tymczyszyn, E. Elizabeth; Semorile, Liliana C; Valdes La Hens, Danay; Delfederico, Lucrecia; Hollmann, Axel; Bravo-Ferrada, Barbara.
Título: Lactobacillus plantarum as a malolactic starter culture in winemaking: a new (old) player?
Fonte: Electron. j. biotechnol;38:10-18, Mar. 2019. tab.
Idioma: en.
Projeto: Universidad Nacional de Quilmes; . Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT ­ MINCyT, Argentina; . Comisión Nacional de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-BA, Argentina).
Resumo: Malolactic fermentation (MLF) is a process in winemaking responsible for the conversion of L-malic acid to L-lactic acid and CO2, which reduces the total acidity, improves the biological stability, and modifies the aroma profile of wine. MLF takes place during or after alcoholic fermentation and is carried out by one or more species of lactic acid bacteria (LAB), which are either present in grapes and cellars or inoculated with malolactic starters during the winemaking process. Although the main bacterium among LAB used in commercial starter cultures for MLF has traditionally been Oenococcus oeni, in the last decade, Lactobacillus plantarum has also been reported as a malolactic starter, and many works have shown that this species can survive and even grow under harsh conditions of wine (i.e., high ethanol content and low pH values). Furthermore, it has been proved that some strains of L. plantarum are able to conduct MLF just as efficiently as O. oeni. In addition, L. plantarum exhibits a more diverse enzymatic profile than O. oeni, which could play an important role in the modification of the wine aroma profile. This enzymatic diversity allows obtaining several starter cultures composed of different L. plantarum biotypes, which could result in distinctive wines. In this context, this review focuses on showing the relevance of L. plantarum as a MLF starter culture in winemaking.
Descritores: Vinho/microbiologia
Lactobacillus plantarum/metabolismo
Fermentação
Malatos/metabolismo
-Vitis/microbiologia
Odorantes
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: lil-775120
Autor: Šuranská, Hana; Vránová, Dana; Omelková, Jiřina.
Título: Isolation, identification and characterization of regional indigenous Saccharomyces cerevisiae strains
Fonte: Braz. j. microbiol;47(1):181-190, Jan.-Mar. 2016. tab, graf.
Idioma: en.
Projeto: MŠMT ČR.
Resumo: Abstract In the present work we isolated and identified various indigenous Saccharomyces cerevisiae strains and screened them for the selected oenological properties. These S. cerevisiae strains were isolated from berries and spontaneously fermented musts. The grape berries (Sauvignon blanc and Pinot noir) were grown under the integrated and organic mode of farming in the South Moravia (Czech Republic) wine region. Modern genotyping techniques such as PCR-fingerprinting and interdelta PCR typing were employed to differentiate among indigenous S. cerevisiae strains. This combination of the methods provides a rapid and relatively simple approach for identification of yeast of S. cerevisiae at strain level. In total, 120 isolates were identified and grouped by molecular approaches and 45 of the representative strains were tested for selected important oenological properties including ethanol, sulfur dioxide and osmotic stress tolerance, intensity of flocculation and desirable enzymatic activities. Their ability to produce and utilize acetic/malic acid was examined as well; in addition, H2S production as an undesirable property was screened. The oenological characteristics of indigenous isolates were compared to a commercially available S. cerevisiae BS6 strain, which is commonly used as the starter culture. Finally, some indigenous strains coming from organically treated grape berries were chosen for their promising oenological properties and these strains will be used as the starter culture, because application of a selected indigenous S. cerevisiae strain can enhance the regional character of the wines.
Descritores: Saccharomyces cerevisiae/classificação
Saccharomyces cerevisiae/isolamento & purificação
Vitis/microbiologia
-Ácido Acético/metabolismo
Aderência Bacteriana
República Tcheca
Impressões Digitais de DNA
Tolerância a Medicamentos
Etanol/toxicidade
Sulfeto de Hidrogênio/metabolismo
Tipagem Molecular
Técnicas de Tipagem Micológica
Malatos/metabolismo
Pressão Osmótica
Reação em Cadeia da Polimerase
Estresse Fisiológico
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/fisiologia
Dióxido de Enxofre/toxicidade
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
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Id: lil-600653
Autor: Vasconcelos, Paulo Roberto Cavalcante de; Costa Neto, Claudio Duarte da; Vasconcelos, Raquel Cavalcante de; Souza, Pedro Paulo Chaves de; Vasconcelos, Paulo Roberto Leitão; Guimarães, Sérgio Botelho.
Título: Effect of glutamine on the mRNA level of key enzymes of malate-aspartate shuttle in the rat intestine subjected to ischemia reperfusion / Efeito da glutamina sobre o nível de RNA Mensageiro das enzimas-chave do ciclo malato-aspartato no intestino de ratos submetidos à isquemia e reperfusão
Fonte: Acta cir. bras;26(supl.1):26-31, 2011. ilus, graf.
Idioma: en.
Resumo: PURPOSE: To determine the effects of oral L-glutamine (L-Gln) and the dipeptide l-alanyl-glutamine (L-Ala-Gln) upon the activity of the malate-aspartate shuttle in the rat distal small intestine following ischemia and reperfusion. METHODS: Seventy-two Wistar rats (350-400g), were randomized in 2 groups (n = 36): group S (Sham) and Group T (Treatment) and divided into 12 subgroups (n = 6): A-A6, and B1-B6. The subgroups A1-A3 were subjected to sham procedures at 30 and 60 minutes. Thirty minutes before the study, rats were treated with calcium caseinate, 0.5g/Kg (subgroups A1, A4, B1, B4), L-Gln, 0.5g / kg (subgroups A2, A5, B2 and B5) or L-Ala-Gln, 0.75g/Kg (subgroups A3, A6, B3, B6), administered by gavage. Ischemia was achieved by clamping the mesenteric vessels, delimiting a segment of bowel 5 cm long and 5 cm apart from the ileocecal valve. Samples were collected 30 and 60 minutes after start of the study for real-time PCR assay of malate dehydrogenases (MDH1-2) and aspartate-aminotransferases (GOT1-2) enzymes. RESULTS: Tissue MDH and GOT mRNA expression in intestinal samples from rats preconditioned with either L-Gln or L-Ala-Gln showed no significant differences both during ischemia and early reperfusion. CONCLUSION: Activation of the malate-aspartate shuttle system appears not to be the mechanism of glutamine-mediated elevation of glucose oxidation in rat intestine during ischemia/reperfusion injury.

OBJETIVO: Determinar os efeitos da administração oral de L-glutamina (L-Gln) e do dipeptídeo L-alanil-glutamina (L-Ala-Gln) sobre a atividade do ciclo malato-aspartato no intestino delgado distal de ratos após isquemia/reperfusão. MÉTODOS: Setenta e dois ratos Wistar (350-400g) foram randomizados em 2 grupos (n = 36): T grupo S (Sham) e grupo (Tratamento) e distribuídos em 12 subgrupos (n = 6): A-A6, e B1-B6. Os subgrupos A1-A3 foram submetidos a procedimentos "sham" aos 30 e 60 minutos. Trinta minutos antes do estudo, os ratos foram tratados com caseinato de cálcio, 0,5 g/kg (subgrupos A1, A4, B1 e B4), L-Gln, 0,5 g/kg (subgrupos A2, A5, B2 e B5) ou L-Ala -Gln, 0,75g/kg (subgrupos A3, A6, B3, B6), administrado por gavagem. A isquemia foi obtida por pinçamento dos vasos mesentéricos, delimitando um segmento do intestino cinco centímetros de comprimento e 5 cm da válvula ileocecal. Amostras foram coletadas aos 30-60 minutos para ensaio de PCR em tempo real das enzimas malato desidrogenases (MDH1-2), aspartato-aminotransferase (GOT1-2). RESULTADOS: A expressão de MDH e GOT mRNA nas amostras provenientes do intestino delgado de ratos pré-condicionados com L-Gln ou L-Ala-Gln não apresentou diferenças significativas, tanto durante a isquemia como na fase inicial de reperfusão. CONCLUSÃO: Ativação do ciclo malato-aspartato não parece ser o mecanismo de elevação glutamina-mediada da oxidação da glicose no intestino de ratos durante a isquemia / reperfusão.
Descritores: Ácido Aspártico/metabolismo
Glutamina/farmacologia
Intestino Delgado/irrigação sanguínea
Malatos/metabolismo
RNA Mensageiro/sangue
Traumatismo por Reperfusão/prevenção & controle
-Aspartato Aminotransferases/sangue
Aspartato Aminotransferases/genética
Modelos Animais de Doenças
Dipeptídeos/farmacologia
Intestino Delgado/enzimologia
Malato Desidrogenase/sangue
Malato Desidrogenase/genética
Distribuição Aleatória
Ratos Wistar
Reação em Cadeia da Polimerase em Tempo Real
Traumatismo por Reperfusão/enzimologia
Fatores de Tempo
Limites: Animais
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Texto completo SciELO Venezuela
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Id: lil-548543
Autor: Hernández, Joaquín; Castillo, Cristina; Méndez, Jesús; Vázquez, Patricia; Pereira, Víctor; Llena, Jaime; López Alonso, Marta; Benedito, José Luis.
Título: Comparación del Efecto de la adición de malato vs monensina sobre parámetros bioquímicos en terneros en crecimiento / Comparison between malate vs monensin adition on biochemicals parameters in growing steers
Fonte: Rev. cient. (Maracaibo);17(5):514-520, sept.-oct. 2007. tab.
Idioma: es.
Resumo: El objetivo del presente trabajo fue evaluar “ in vivo ” las repercusiones que tiene la adición de malato sódico sobre parámetros del medio interno, comparándolos con los resultados obtenidos al aplicar monensina sódica. Los parámetros sanguíneos estudiados fueron: glucosa, colesterol, triglicéridos, ácidos grasos libres, y las enzimas aspartato amino transferasa (ASAT), amilasa y gamma glutamil transpeptidasa (GGT). El estudio fue realizado con 13 animales, 8 de ellos recibieron malato sódico y 5 animales monensina sódica, extrayendo 6 muestras a cada animal, una toma basal (toma 1), y a los 3 (toma 2), 7 (toma 3), 21 (toma 4), 46 (toma 5) y 57 días (toma 6). Los resultados obtenidos muestran muy pocas diferencias entre ambos grupos y evoluciones parecidas, con variaciones entre grupos en el día 3 (ácidos grasos libres), día 7 (GGT), en el día 21 (amilasa) y en el día 46 (amilasa y GGT). En cuanto a las evoluciones de los parámetros a lo largo del experimento, colesterol, triglicéridos, amilasa y ASAT son los cuatro parámetros que presentan cambios estadísticos, con evoluciones similares en ambos grupos.

Effects of sodium malate addition on selected blood parameters, compared with the monensin addition were evaluated in this study. Serum glucose, triglycerides, cholesterols, free fatty acids (FFA), and the enzymes aspartate amino transferase (ASAT), amylase and gamma glutamyl transpeptidase (GGT) were studied. Thirteen steers, distributed in two different groups were used, one group (n=8) received sodium malate, and another group (n=5) received monensin and considered for us as a control group. Six samplings were obtained for each animal, at day 0 (before addition), and at days 3; 7; 21; 46 and 57 (after addition), respectively. Results obtained showed a similar evolution in both groups with small differences between them, at day 3 (FFA), at day 7 (GGT), at day 21 (amylase) and at day 46 (GGT and amylase). In relation with the evolution, we have seen similar statistical changes in both groups for cholesterol, triglycerides, amylase and ASAT assays.
Descritores: Enzimas/análise
Malatos/efeitos adversos
Monensin/efeitos adversos
Reações Bioquímicas/efeitos adversos
-Medicina Veterinária
Limites: Bovinos
Animais
Tipo de Publ: Estudo Comparativo
Estudo de Avaliação
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Id: lil-82990
Autor: Palma, M. S; Kokubun, E; Sibuya, C. Y; Santos, J. W; Freire, P. M.
Título: Regulatory mechanisms of blood lactate production during exercise in man
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;22(11):1329-32, 1989. ilus.
Idioma: en.
Conferência: Apresentado em: Annual Meeting of the Federaçäo de Sociedades de Biologia Experimental, 4, Caxambu, June 28-July 2, 1989.
Projeto: CNPq.
Resumo: During cycloergometric exercise at progressively increasing loads, blood lactate concentration increased about 12-fold. Pyruvate concentration decreased initially(for loads of 50-75 W), increased with loads of 75 to 125 W and then decreased again until the end of exercise. the malate concentration increased abruptly between 50 and 75 W, followed by a slow decline; citrate increased about nine-fold as the exercise load was increased to 125 W and then fell sharply. Thus, the production of lactate during low-intensity exercise seems to occur by the "mass-action effect" caused by enhanced glycolysis, whereas with moderate loads the glycolysis rate is very much reduced and most of the lactate production seems to involve the action of the malate-aspartate shuttle. For high-intensity exercise, both mechanisms appear to participate in lactate production
Descritores: Teste de Esforço
Lactatos/sangue
-Cromatografia Líquida de Alta Pressão
Citratos/sangue
Lactatos/metabolismo
Malatos/sangue
Músculos/metabolismo
Consumo de Oxigênio
Piruvatos/sangue
Limites: Humanos
Masculino
Responsável: BR26.1 - Biblioteca Central



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