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Id: biblio-1013463
Autor: Valente, Acrisio Sales; Lustosa, Gustavo Porto; Mota, Lia Alves Martins; Lima, Adriano; Mesquita, Fernando Antônio de; Gondim, Aloísio; Rodrigues, Fábio Alércio; Pompeu, Ronald Guedes; Branco, Klébia Castelo.
Título: Comparative analysis of myocardial protection with htk solution and hypothermic hyperkalemic blood solution in the correction of acyanogenic congenital cardiopathies - a randomized study
Fonte: Rev. bras. cir. cardiovasc = Braz. j. cardiovasc. surg. (impr.);34(3):271-278, Jun. 2019. tab, graf.
Idioma: en.
Resumo: Abstract Objective: The goal of the present study was to compare the myocardial protection obtained with histidine-tryptophan-ketoglutarate (HTK) cardioplegic solution (Custodiol®) and with intermittent hypothermic blood solution. Methods: Two homogenous groups of 25 children with acyanotic congenital heart disease who underwent total correction with mean aortic clamping time of 60 minutes were evaluated in this randomized study. Troponin and creatine kinase-MB curves, vasoactive-inotropic score, and left ventricular function were obtained by echocardiogram in each group. The values were correlated and presented through graphs and tables after adequate statistical treatment. Results: It was observed that values of all the studied variables varied over time, but there was no difference between the groups. Conclusion: We conclude that in patients with acyanotic congenital cardiopathies submitted to total surgical correction, mean aortic clamping time around one hour, and cardiopulmonary bypass with moderate hypothermia, the HTK crystalloid cardioplegic solution offers the same myocardial protection as the cold-blood hyperkalemic cardioplegic solution analyzed, according to the variables considered in our study model.
Descritores: Soluções Cardioplégicas/uso terapêutico
Cardiopatias Congênitas/cirurgia
-Cloreto de Potássio/uso terapêutico
Procaína/uso terapêutico
Valores de Referência
Fatores de Tempo
Troponina/análise
Ecocardiografia
Método Duplo-Cego
Estudos Prospectivos
Reprodutibilidade dos Testes
Análise de Variância
Função Ventricular Esquerda
Resultado do Tratamento
Estatísticas não Paramétricas
Substâncias Protetoras/uso terapêutico
Creatina Quinase Forma MB/análise
Duração da Cirurgia
Glucose/uso terapêutico
Cardiopatias Congênitas/fisiopatologia
Manitol/uso terapêutico
Limites: Humanos
Masculino
Feminino
Recém-Nascido
Lactente
Tipo de Publ: Ensaio Clínico Controlado Aleatório
Responsável: BR1.1 - BIREME


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Id: biblio-1137349
Autor: Mercan, Ilker; Dereli, Yuksel; Topcu, Cemile; Tanyeli, Omer; Isik, Mehmet; Gormus, Niyazi; Ozturk, Elifnur Yildirim.
Título: Comparison between the effects of bretschneiders htk solution and cold blood cardioplegia on systemic endothelial functions in patients who undergo coronary artery bypass surgery: a prospective randomized and controlled trial
Fonte: Rev. bras. cir. cardiovasc = Braz. j. cardiovasc. surg. (impr.);35(5):634-643, Sept.-Oct. 2020. tab, graf.
Idioma: en.
Resumo: Abstract Objective: To investigate the effects of Bretschneider's histidine-tryptophan-ketoglutarate (HTK) solution and cold blood cardioplegia on systemic endothelial functions. Methods: A total of 50 patients who underwent isolated coronary artery bypass surgery between March 2018 and May 2018 were randomly divided into two groups - group 1 (Bretschneider's HTK solution, n=25) and group 2 (cold blood cardioplegia, n=25). Data related to the indicators of endothelial dysfunction were recorded. Flow-mediated dilation was measured together with the assessment of the values of endothelin-1, von Willebrand factor, and asymmetric dimethylarginine to identify endothelial dysfunction. Then, the two groups were compared regarding these values. Results: The most significant result of our study was that the endothelin-1 level was significantly higher in group 2 than in group 1 (P<0.001). The value of flow-mediated dilation was found to increase to a lesser degree on the postoperative days compared to the value at the day of admission in group 1 (P=0.002 and P=0.030, respectively). Conclusion: Cardiopulmonary bypass leads to endothelial dysfunction. Our results revealed that Bretschneider's HTK solution causes less severe endothelial injury than cold blood cardioplegia.
Descritores: Soluções Cardioplégicas/uso terapêutico
Ponte de Artéria Coronária
Parada Cardíaca Induzida
-Cloreto de Potássio
Procaína
Estudos Prospectivos
Glucose
Manitol
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Research Support, Non-U.S. Gov't
Ensaio Clínico Controlado Aleatório
Responsável: BR1.1 - BIREME


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Id: biblio-1096849
Autor: Chirinos, A; Del Moral, E; Martínez, L.
Título: Dolor lumbar somático tratado con terapia neural y ozono paravertebral / Somatic low back pain treated with neural therapy and paravertebral ozone
Fonte: Revista Digital de Postgrado;6(1):38-45, jun. 2017. tab.
Idioma: es.
Resumo: La presente investigación tuvo como objetivo general evaluar el efecto de la terapia neural e infiltración paravertebral segmentaria con ozono en el dolor lumbar somático de los pacientes que asistieron a la consulta de terapia del dolor en el Hospital Dr. Pedro García Clara, Ciudad Ojeda Estado Zulia-Venezuela, en el periodo comprendido del 01 de abril al 31 de mayo del 2016. Fue un estudio prospectivo, explicativo-comparativo y el diseño fue cuasi-experimental. Se seleccionaron 60 pacientes de ambos sexos entre edades comprendidas de 18 a 60 años, se dividieron en dos grupos de 30 pacientes, al primer grupo denominado A, se le realizó terapia neural segmentaria lumbar utilizando procaína al 1% y consecutivamente se le aplicó infiltración paravertebral con ozono a una concentración de 15ug; y al grupo B, se le realizó terapia neural con lidocaína al 1% y posteriormente se le aplicó la infiltración paravertebral con ozono a una concentración de 15ug, ambos grupos con un volumen de 10mL. Para el análisis de los datos se utilizó estadística descriptiva e inferencial, específicamente la prueba t de Student para las muestras relacionadas. Resultados: indicaron que en ambas terapias, al evaluar el dolor con la escala visual análoga, se encontraron diferencias estadísticamente significativas (p<0,05), en ambos grupos. Conclusiones: no hubo diferencias estadísticamente significativas con respecto a los cambios hemodinámicos y la disminución del dolor fue significativa en ambos grupos, pero más notable en el grupo A(AU)

The present study was aimed at evaluating the overall effect of neural therapy and segmental paravertebral ozone infiltration in lumbar somatic pain patients attending the consultation of pain therapy Dr. Pedro Garcia Clara Hospital of Ciudad Ojeda Estado Zulia-Venezuela, in the period from 01 April to 31 May 2016. It was a comparative-explanatory, prospective study design was quasi-experimental, 60 patients of either sex between aged from 18 to 60 years were selected, they were divided into two groups of 30 patients, the first group called A, underwent neural therapy lumbar segmental using procaine 1% and consecutively was applied paravertebral infiltration with ozone at a concentration of 15ug; and group B, underwent neural therapy with lidocaine 1% and was subsequently applied the paravertebral infiltration with ozone at a concentration of 15ug, both groups with a volume of 10mL. For data analysis the mean, standard deviation, and inferential statistics, specifically the Student t test for related samples was used. Results: indicated that both therapies, to assess pain with visual analogue scale, statistically significant differences (p<0.05) in both groups. Conclusions: There were no statistically significant differences with respect to hemodynamic changes and pain reduction was significant in both groups, but more remarkable in group A(AU)
Descritores: Procaína/uso terapêutico
Dor Lombar/terapia
Lidocaína/uso terapêutico
Sistema Nervoso/fisiopatologia
-Quimioterapia Combinada
Anestésicos/administração & dosagem
Limites: Humanos
Tipo de Publ: Estudo de Avaliação
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Id: biblio-1001091
Autor: Büyük, Başak; Demirci, Tuba; Adalı, Yasemen; Eroğlu, Hüseyin Avni.
Título: A new organ preservation solution for static cold storage of the liver. Amniotic fluid
Fonte: Acta cir. bras;34(4):e201900402, 2019. tab, graf.
Idioma: en.
Projeto: The Scientific and Technological Research Council of Turkey.
Resumo: Abstract Purpose: To evaluate the effect of amniotic fluid in liver preservation in organ transplantation, and compare it with standard preservation solutions. Methods: The groups consisted of Group 1: Ringer Lactate (RL) group, Group 2: HTK group, Group 3: UW group, Group 4: AF group. The livers of rats from Group 1, 2, 3, and 4 were perfused and placed into falcon tubes containing RL, HTK, UW, and AF solutions at +4‎°C, respectively. The tubes were stored for 12 hours in the refrigerator at +4°C. Tissue samples were taken at the 6th and 12th hours for histopathological examinations of the perfused livers, and storage solutions for biochemical analyzes at 6th and 12th hours. Results: AF was shown to maintain organ viability by reducing the number of cells undergoing apoptosis. Histopathological changes such as sinusoidal dilatation, hydropic degeneration, and focal necrosis were found to be similar to the groups in which the standard organ preservation solutions were used. Additionally, the results of INOS, IL-10, and TNF-α,which were evaluated immunohistochemically, have been shown to be similar to the UW and HTK groups. Conclusions: AF provided conservation similar to UW and HTK in the 12-hour liver SCS process. The fact that apoptosis values are comparable to standard preservation solutions supports the success of AF in the cold storage of the liver.
Descritores: Criopreservação/métodos
Soluções para Preservação de Órgãos/farmacologia
Líquido Amniótico
Fígado/irrigação sanguínea
Fígado/patologia
-Preservação de Órgãos/métodos
Cloreto de Potássio/farmacologia
Procaína/farmacologia
Valores de Referência
Fatores de Tempo
Sobrevivência de Tecidos
Imuno-Histoquímica
Traumatismo por Reperfusão/prevenção & controle
Distribuição Aleatória
Reprodutibilidade dos Testes
Fator de Necrose Tumoral alfa/análise
Interleucina-10/análise
Ratos Wistar
Marcação In Situ das Extremidades Cortadas
Óxido Nítrico Sintase Tipo II/análise
Solução de Ringer/farmacologia
Glucose/farmacologia
Manitol/farmacologia
Limites: Animais
Masculino
Tipo de Publ: Estudo Comparativo
Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-886210
Autor: Özer, Nazmi; Bülbüloğlu, Ertan; Yormaz, Serdar; Ece, İlhan.
Título: Evaluation of silybum marinaum efficacy on University of Wisconsin and histidine-tryptophan-ketoglutarate solutions latter the damage of the perfused liver
Fonte: Acta cir. bras;32(6):407-417, June 2017. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To investigate the hepatoprotective and antioxidant effeicacies of Silybum marianum's (silymarin, S) on University of Wisconsin (UW) and histidinetryptophan-ketoglutarate (HTK) preservation solutions. Methods: Thirty two Wistar albino adult male rats were used. Group 1: UW group, Group 2: UW + Silymarin group(S), Group 3: HTK group, Group 4: HTK + silymarin group (S), respectively. Silymarin was enforced intraperitoneally before the surgery. Biopsies were enforced in 0, 6 and 12.hours to investigate. Results: Biochemical parameters examined in alanine aminotransferase (ALT), furthermore superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in rats were also evaluated. Detected histopathological changings were substantially declining in the groups that received silymarin, cellular damage was decreased significantly in HTK + Silymarin group, according to other groups. It has been identified as the most effective group was HTK + silymarin group in evaluation of ALT, electron microscopic results, also decreased MDA and elevated in SOD, and CAT activity. Caspase 3 analysis showed a substantial lower apoptosis ratio in the silymarin groups than in the non-performed groups (p<0.05). Conclusion: Histidinetryptophan-ketoglutarate+silymarin group provides better hepatoprotection than other groups, by decreasing the hepatic pathologic damage, delayed changes that arise under cold ischemic terms.
Descritores: Silimarina/uso terapêutico
Soluções para Preservação de Órgãos
Substâncias Protetoras/uso terapêutico
Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico
Antioxidantes/uso terapêutico
-Cloreto de Potássio
Procaína
Rafinose
Imuno-Histoquímica
Adenosina
Alopurinol
Ratos Wistar
Modelos Animais de Doenças
Glucose
Glutationa
Insulina
Manitol
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-877091
Autor: Avellaneda López, Dora Lucía.
Título: Evaluación experimental en roedores, de la toxicidad de la procaína en algunos esquemas de utilización como neural terapéutico / Experimental evaluation in rodents of the toxicity of procaine in some schemes of use as therapeutic neural.
Fonte: Bogotá; s.n; 2011. 75 p. tab.
Idioma: es.
Tese: Apresentada a Universidad Nacional de Colombia para obtenção do grau de Maestría.
Resumo: La procaína es el fármaco para la realización de la terapia neural. Su perfil de seguridad ha sido descrito para su uso como anestésico local, sin embargo, la literatura revisada no aporta información suficiente para su uso como neural terapéutico. El objetivo del presente trabajo es contribuir con el conocimiento sobre las bases teóricas de la Terapia Neural, mediante la evaluación de la toxicidad de la Procaína en roedores, según el esquema de uso como neural terapéutico. Se realizó un estudio de experimentación animal para evaluar la neurotoxicidad de la Procaína en administración repetida por vía intradérmica. El comportamiento animal se observó una hora después de cada aplicación y durante el mes siguiente, mediante una batería de pruebas observacionales para evaluar signos clínicos de neurotoxicidad. En ninguna de las pruebas aplicadas se observaron cambios sugestivos de neurotoxicidad, con lo que se puede concluir que la Procaína es segura al ser administrada en dosis bajas por vía intradérmica, teniendo en cuenta que hacen falta estudios para establecer el perfil completo de la Procaína como neural terapéutico.
Descritores: Procaína/toxicidade
Terapias Complementares
Modelos Animais
-Medidas de Toxicidade
Limites: Animais
Camundongos
Responsável: BR1.1 - BIREME


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Id: lil-781416
Autor: Li, X W; Lin, Y Z; Lin, H; Huang, J B; Tang, X M; Long, X M; Lu, W J; Wen, Z K; Liang, J; Li, D Y; Zhao, X F.
Título: Histidine-tryptophan-ketoglutarate solution decreases mortality and morbidity in high-risk patients with severe pulmonary arterial hypertension associated with complex congenital heart disease: an 11-year experience from a single institution
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;49(6):e5208, 2016. tab.
Idioma: en.
Projeto: Natural Science Foundation of China; . Natural Science Foundation of Guangxi; . Guangxi key scientific and technological project; . Guangxi Health Department.
Resumo: Cardioplegic reperfusion during a long term ischemic period interrupts cardiac surgery and also increases cellular edema due to repeated solution administration. We reviewed the clinical experiences on myocardial protection of a single perfusion with histidine-tryptophan-ketoglutarate (HTK) for high-risk patients with severe pulmonary arterial hypertension associated with complex congenital heart disease. This retrospective study included 101 high-risk patients undergoing arterial switch operation between March 2001 and July 2012. We divided the cohort into two groups: HTK group, myocardial protection was carried out with one single perfusion with HTK solution; and St group, myocardial protection with conventional St. Thomas' crystalloid cardioplegic solution. The duration of cardiopulmonary bypass did not differ between the two groups. The mortality, morbidity, ICU stay, post-operative hospitalization time, and number of transfusions in HTK group were lower than those in St group (P<0.05). Univariate and multivariate analysis showed that HTK is a statistically significant independent predictor of decreased early mortality and morbidity (P<0.05). In conclusion, HTK solution seems to be an effective and safe alternative to St. Thomas' solution for cardioplegic reperfusion in high-risk patients with complex congenital heart disease.
Descritores: Soluções Cardioplégicas/uso terapêutico
Ponte Cardiopulmonar/métodos
Parada Cardíaca Induzida/métodos
Cardiopatias Congênitas/cirurgia
Hipertensão Pulmonar/cirurgia
-Análise de Variância
Glucose/uso terapêutico
Cardiopatias Congênitas/mortalidade
Hipertensão Pulmonar/mortalidade
Soluções Isotônicas/uso terapêutico
Estimativa de Kaplan-Meier
Manitol/uso terapêutico
Perfusão/métodos
Cloreto de Potássio/uso terapêutico
Procaína/uso terapêutico
Reprodutibilidade dos Testes
Estudos Retrospectivos
Fatores de Risco
Índice de Gravidade de Doença
Resultado do Tratamento
Limites: Humanos
Masculino
Feminino
Lactente
Pré-Escolar
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: lil-731653
Autor: Martínez, Jorge; Bächler, Jean P; Moisan, Fabrizio; Torres, Javiera; Duarte, Ignacio; Pérez, Rosa María; Benítez, Carlos; Arrese, Marco; Domínguez, Pilar; Guerra, Juan F; Jarufe, Nicolás.
Título: ¿Influye el medio de preservación del injerto en los resultados y costos del trasplante hepático de donante fallecido?: Comparación de dos soluciones de preservación / Outcomes using two preservation solutions (UW/HTK) in liver transplantation from brain death donors
Fonte: Rev. méd. Chile;142(10):1229-1237, oct. 2014. ilus, tab.
Idioma: es.
Resumo: Background: Preservation solutions are critical for organ transplantation. In liver transplant (LT), the solution developed by the University Of Wisconsin (UW) is the gold-standard to perfuse deceased brain death donor (DBD) grafts. Histidine-Tryptophan-Ketoglutarate (HTK), formerly a cardioplegic infusion, has been also used in solid organ transplantation. Aim: To compare the outcomes of LT in our center using either HTK or UW solution. Patients and Methods: Retrospective study including 93 LT DBD liver grafts in 89 patients transplanted between March 1994 and July 2010. Forty-eight grafts were preserved with UW and 45 with HTK. Donor and recipient demographics, total infused volume, cold ischemia time, post-reperfusion biopsy, liver function tests, incidence of biliary complications, acute rejection and 12-month graft and patient survival were assessed. Preservation solution costs per liver graft were also recorded. Results: Donor and recipient demographics were similar. When comparing UW and HTK, no differences were observed in cold ischemia time (9.6 ± 3 and 8.7 ± 2 h respectively, p = 0.23), biliary complications, the incidence of acute rejection, primary or delayed graft dysfunction. Histology on post-reperfusion biopsies revealed no differences between groups. The infused volume was significantly higher with HTK than with UW (9 (5-16) and 6 (3-11) l, p < 0.001). The cost per procurement was remarkably lower using HTK. Conclusions: Perfusion of DBD liver grafts with HTK is clinically equivalent to UW, with a significant cost reduction.
Descritores: Fígado
Transplante de Fígado/métodos
Soluções para Preservação de Órgãos
Preservação de Órgãos/instrumentação
-Adenosina
Alopurinol
Morte Encefálica
Glucose
Glutationa
Sobrevivência de Enxerto/efeitos dos fármacos
Sobrevivência de Enxerto/fisiologia
Insulina
Falência Hepática/patologia
Manitol
Cloreto de Potássio
Procaína
Rafinose
Estudos Retrospectivos
Doadores de Tecidos
Limites: Adulto
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Tipo de Publ: Estudo Comparativo
Responsável: CL1.1 - Biblioteca Central


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Braile, Domingo Marcolino
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Id: lil-719409
Autor: Oliveira, Marcos Aurélio Barboza de; Brandi, Antônio Carlos; Santos, Carlos Alberto dos; Botelho, Paulo Henrique Husseni; Cortez, José Luís Lasso; Godoy, Moacir Fernandes de; Braile, Domingo Marcolino.
Título: Comparação da dimensão fractal e entropia de Shannon em miócitos de ratos submetidos à cardioplegia utilizando solução histidina-triptofano-cetoglutarato e histidina-triptofano com glutamato / Comparison of fractal dimension and Shannon entropy in myocytes from rats treated with histidine-tryptophan-glutamate and histidine-tryptophan cetoglutarate
Fonte: Rev. bras. cir. cardiovasc;29(2):156-162, Apr-Jun/2014. tab, graf.
Idioma: pt.
Resumo: Introdução: As soluções que provocam parada cardíaca eletiva estão em constante evolução, porém, o composto ideal ainda não foi encontrado. Os autores comparam uma nova solução cardioplégica com histidina-triptofano-glutamato (Grupo 2) com histidina-triptofano-cetoglutarato (Grupo 1) em modelo de coração isolado de rato. Objetivo: Quantificar a dimensão fractal e entropia de Shannon em miócitos de rato submetidos à cardioplegia utilizando solução histidina-triptofano com glutamato em modelo experimental, considerando-se os marcadores caspase, IL-8 e Ki-67. Métodos: Vinte ratos machos de raça Wistar foram anestesiados e heparinizados. O tórax foi aberto, realizado cardiectomia e infundido 40 ml/Kg de solução cardioplégica apropriada. Os corações foram mantidos por 2 horas na mesma solução a 4ºC e, após esse período, colocados em aparato de Langendorff por 30 minutos com solução de Ringer Locke. Foram feitas análises imunohistoquímicas para caspase, IL-8 e KI-67. Resultados: A dimensão fractal e a entropia de Shannon dos corações submetidos à parada cardíaca eletiva nos grupos 1 e 2 não foram diferentes. Conclusão: A quantidade de informações avaliada pela entropia de Shannon e a distribuição das mesmas (dada pela dimensão fractal) nas lâminas de coração de rato submetidas à cardioplegia com solução histidina-triptofano-acetoglutarato ou histidina-triptofano-glutamato não foram diferentes, o que mostra que a solução de histidina-triptofano-glutamato é tão boa quanto a histidina-triptofano-cetoglutarato na preservação dos miócitos em modelo de coração isolado de rato. .

Introduction: Solutions that cause elective cardiac arrest are constantly evolving, but the ideal compound has not yet been found. The authors compare a new cardioplegic solution with histidine-tryptophan-glutamate (Group 2) and other one with histidine-tryptophan-cetoglutarate (Group 1) in a model of isolated rat heart. Objective: To quantify the fractal dimension and Shannon entropy in rat myocytes subjected to cardioplegia solution using histidine-tryptophan with glutamate in an experimental model, considering the caspase markers, IL-8 and KI-67. Methods: Twenty male Wistar rats were anesthetized and heparinized. The chest was opened, the heart was withdrawn and 40 ml/kg of cardioplegia (with histidine-tryptophan-cetoglutarate or histidine-tryptophan-glutamate solution) was infused. The hearts were kept for 2 hours at 4ºC in the same solution, and thereafter placed in the Langendorff apparatus for 30 min with Ringer-Locke solution. Analyzes were performed for immunohistochemical caspase, IL-8 and KI-67. Results: The fractal dimension and Shannon entropy were not different between groups histidine-tryptophan-glutamate and histidine-tryptophan-acetoglutarate. Conclusion: The amount of information measured by Shannon entropy and the distribution thereof (given by fractal dimension) of the slices treated with histidine-tryptophan-cetoglutarate and histidine-tryptophan-glutamate were not different, showing that the histidine-tryptophan-glutamate solution is as good as histidine-tryptophan-acetoglutarate to preserve myocytes in isolated rat heart. .
Descritores: Soluções Cardioplégicas/farmacologia
Ácido Glutâmico/farmacologia
Parada Cardíaca Induzida/métodos
Miócitos Cardíacos/efeitos dos fármacos
-Caspases/análise
Modelos Animais de Doenças
Entropia
Fractais
Glucose/farmacologia
Coração/efeitos dos fármacos
Imuno-Histoquímica
/análise
INTERLEUKIN-ABDOMINAL NEOPLASMS/análise
/análise
KI-ABDOMEN, ACUTEABDOMINAL INJURIES ANTIGEN/análise
Manitol/farmacologia
Cloreto de Potássio/farmacologia
Procaína/farmacologia
Ratos Wistar
Reprodutibilidade dos Testes
Fatores de Tempo
Limites: Animais
Masculino
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: lil-705768
Autor: Olivoto, R.R.; Damiani, C.E.N.; Kassouf Silva, I.; Lofrano-Alves, M.S.; Oliveira, M.A.; Fogaça, R.T.H..
Título: Effects of eugenol on resting tension of rat atria
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(4):328-333, 8/4/2014. graf.
Idioma: en.
Resumo: In cardiac and skeletal muscle, eugenol (μM range) blocks excitation-contraction coupling. In skeletal muscle, however, larger doses of eugenol (mM range) induce calcium release from the sarcoplasmic reticulum. The effects of eugenol are therefore dependent on its concentration. In this study, we evaluated the effects of eugenol on the contractility of isolated, quiescent atrial trabeculae from male Wistar rats (250-300 g; n=131) and measured atrial ATP content. Eugenol (1, 3, 5, 7, and 10 mM) increased resting tension in a dose-dependent manner. Ryanodine [100 µM; a specific ryanodine receptor (RyR) blocker] and procaine (30 mM; a nonspecific RyR blocker) did not block the increased resting tension induced by eugenol regardless of whether extracellular calcium was present. The myosin-specific inhibitor 2,3-butanedione monoxime (BDM), however, reversed the increase in resting tension induced by eugenol. In Triton-skinned atrial trabeculae, in which all membranes were solubilized, eugenol did not change resting tension, maximum force produced, or the force vs pCa relationship (pCa=-log [Ca2+]). Given that eugenol reduced ATP concentration, the increase in resting tension observed in this study may have resulted from cooperative activation of cardiac thin filaments by strongly attached cross-bridges (rigor state).
Descritores: Cálcio/fisiologia
Eugenol/farmacologia
Acoplamento Excitação-Contração/efeitos dos fármacos
Átrios do Coração/efeitos dos fármacos
Força Muscular/efeitos dos fármacos
Contração Miocárdica/efeitos dos fármacos
-Trifosfato de Adenosina/análise
Anestésicos Locais/farmacologia
Eugenol/administração & dosagem
Técnicas In Vitro
Luciferases
Músculo Esquelético/efeitos dos fármacos
Procaína/farmacologia
Ratos Wistar
Rianodina/farmacologia
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME



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