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Id: biblio-1134444
Autor: Mercan, Nedime; Toros, Pelin; Söyler, Gizem; Hanoglu, Azmi; Kükner, Aysel.
Título: Effects of Corchorus olitorius and protacatechuic acid on diabetic rat testis tissue / Efectos de Corchorus olitorius y ácido protocatéquico en el tejido testicular de rata diabética
Fonte: Int. j. morphol;38(5):1330-1335, oct. 2020. tab, graf.
Idioma: en.
Projeto: Near East University.
Resumo: SUMMARY: The aim of this study is to investigate the effects of Protocatechuic acid and Corchorus olitorius on streptozotocin (STZ) induced diabetic rat testis tissue. Randomly selected Wistar Albino rats were divided into five groups as; Diabetes Mellitus, Diabetes Mellitus treated with Corchorus Olitorus (STZ+CO), Diabetes Mellitus treated with Protacatechuic acid (STZ+PCA), Corchorus olitorius (CO), Protocatechuic acid (PCA) and Control. Diabetic model was generated by intraperitoneal injection of 60 mg/kg Streptozotosin. After 48 hours of the STZ injection, blood samples were collected from tail vein in order to measure blood glycose levels. Over 250 mg/dL accepted as diabetic subjets and fed with 250 mg/kg Corchorus olitorius or 20 mg/kg PCA by oral gavage for three weeks. At the end of the experiment, right testes were removed and fixed in 10 % neutral formaldehyde for paraffine embedding. Sections were stained by HE, Masson trichrome, PAS and TUNEL for microscopic evaluation. Control, PCA-only and Corchorus olitorius-only treated group testes tissues showed a normal tissue organization, when degeneration in seminiferous tubules, the vacuolization, seperations in spermatogenic cell series, outpouring of cell groups in the lumen, vesicular body formation, liquid accumulation in the interstitial region and edema were observed in STZ induced diabetic models and untreated groups. Besides, higher amount of TUNEL (+) stained cells were determined in STZ group. On the other hand, blood glucose level and number of TUNEL (+) stained cells were decreased as a result of PCA and Corchorus olitorius treatment. Because of the reduction of blood glucose level and apoptotic cell numbers, PCA and Corchorus olitorius decreace the complications of diabetes mellitus induced rat testis.

RESUMEN: El objetivo de este estudio fue investigar los efectos del ácido protocatéquico y Corchorus olitorius sobre el tejido testicular de rata diabética inducida por estreptozotocina (STZ). Las ratas Wistar Albino fueron seleccionadas al azar y se dividieron en cinco grupos; Diabetes Mellitus, Diabetes Mellitus tratada con Corchorus olitorius (STZ + CO), Diabetes Mellitus tratada con ácido protocatéquico (STZ + PCA), Corchorus olitorius (CO), ácido protocatéquico (PCA) y Control. El modelo diabético se generó por inyección intraperitoneal de 60 mg/kg de estreptozotosina. Después de 48 horas de la inyección de STZ, se recogieron muestras de sangre de la vena de la cola para medir los niveles de glucosa. Niveles mayores a 250 mg/dL fueron considerados como especímenes diabéticos y alimentados con Corchorus olitorius de 250 mg/kg o PCA de 20 mg/kg por sonda oral durante tres semanas. Al final del experimento, se extirparon los testículos derechos y se fijaron en formaldehído neutro al 10 % para la inclusión en parafina. Las secciones se tiñeron con HE, tricromo de Masson, PAS y TUNEL para evaluación microscópica. Los tejidos de los testículos de los grupos control, tratados solo con PCA y con Corchorus olitorius mostraron una organización tisular normal. En cambio en modelos diabéticos inducidos por STZ y grupos no tratados se observó degeneración en los túbulos seminíferos, vacuolización, separaciones en series de células espermatogénicas, efusión de grupos celulares en la luz, formación del cuerpo vesicular, acumulación de líquido en la región intersticial y edema. Además, se determinó una mayor cantidad de células teñidas con TUNEL (+) en el grupo STZ. Por otro lado, el nivel de glucosa en sangre y el número de células teñidas con TUNEL (+) disminuyeron como resultado del tratamiento con PCA y Corchorus olitorius. Debido a la reducción del nivel de glucosa en sangre y el número de células apoptóticas, se observó que PCA y Corchorus olitorius disminuyen las complicaciones de los testículos de rata inducidos por diabetes mellitus.
Descritores: Testículo/efeitos dos fármacos
Extratos Vegetais/farmacologia
Corchorus/química
Diabetes Mellitus Experimental/tratamento farmacológico
Hidroxibenzoatos/farmacologia
-Túbulos Seminíferos/efeitos dos fármacos
Glicemia/análise
Extratos Vegetais/uso terapêutico
Ratos Wistar
Hidroxibenzoatos/uso terapêutico
Limites: Animais
Masculino
Ratos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950715
Autor: Sytar, Oksana; Borankulova, Asel; Hemmerich, Irene; Rauh, Cornelia; Smetanska, Iryna.
Título: Effect of chlorocholine chlorid on phenolic acids accumulation and polyphenols formation of buckwheat plants
Fonte: Biol. Res;47:1-7, 2014. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Effect of chlorocholine chloride (CCC) on phenolic acids composition and polyphenols accumulation in various anatomical parts (stems, leaves and inflorescences) of common buckwheat (Fagopyrum esculentum Moench) in the early stages of vegetation period were surveyed. RESULTS: Treatment of buckwheat seeds with 2% of CCC has been increased content of total phenolics in the stems, leaves and inflorescences. On analyzing the different parts of buckwheat plants, 9 different phenolic acids - vanilic acid, ferulic acid, trans-ferulic acid, chlorogenic acid, salycilic acid, cinamic acid, p-coumaric acid, p-anisic acid, methoxycinamic acid and catechins were identified. The levels of identified phenolic acids varied not only significantly among the plant organs but also between early stages of vegetation period. Same changes as in contents of chlorogenic acid, ferulic acid, trans-ferulic acid were found for content of salycilic acid. The content of these phenolic acids has been significant increased under effect of 2% CCC treatment at the phase I (formation of buds) in the stems and at the phase II (beginning of flowering) in the leaves and then inflorescences respectively. The content of catechins as potential buckwheat antioxidants has been increased at the early stages of vegetation period after treatment with 2% CCC. CONCLUSIONS: The obtained results suggest that influence of CCC on the phenolics composition can be a result of various mechanisms of CCC uptake, transforming and/or its translocation in the buckwheat seedlings.
Descritores: Clormequat/farmacologia
Fagopyrum/efeitos dos fármacos
Polifenóis/biossíntese
Hidroxibenzoatos/metabolismo
-Propionatos
Sementes/efeitos dos fármacos
Sementes/metabolismo
Catequina/análise
Ácido Clorogênico/análise
Cromatografia Líquida de Alta Pressão
Compostos de Tungstênio
Caules de Planta/efeitos dos fármacos
Caules de Planta/metabolismo
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/metabolismo
Fagopyrum/crescimento & desenvolvimento
Fagopyrum/metabolismo
Ácidos Cumáricos/análise
Inflorescência/efeitos dos fármacos
Inflorescência/metabolismo
Éteres de Hidroxibenzoatos/análise
Hidroxibenzoatos/química
Molibdênio
Antioxidantes/análise
Antioxidantes/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950747
Autor: Khan, Alam; Islam, Md Hedayetul; Islam, Md Ekramul; Al-Bari, Md Abdul Alim; Parvin, Mst Shahnaj; Sayeed, Mohammed Abu; Islam, Md Nurul; Haque, Md Ekramul.
Título: Pesticidal and pest repellency activities of rhizomes of Drynaria quercifolia (J. Smith) against Tribolium castaneum (Herbst)
Fonte: Biol. Res;47:1-6, 2014. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: Tribolium castaneum (Herbst) is a harmful pest of stored grain and flour-based products in tropical and subtropical region. In the present study, rhizome of Drynaria quercifolia (J. Smith) was evaluated for pesticidal and pest repellency activities against T. castaneum, using surface film method and filter paper disc method, respectively. In addition, activity of the isolated compound 3,4-dihydroxybenzoic acid was evaluated against the pest. RESULTS: Chloroform soluble fraction of ethanol extract of rhizome of D. quercifolia showed significant pesticidal activity at doses 0.88 to 1.77 mg/cm² and significant pest repellency activity at doses 0.94 to 0.23 mg/cm². No pesticidal and pest repellency activity was found for petroleum ether, ethyl acetate and methanol soluble fractions of ethanol extract as well as for 3,4-dihydroxybenzoic acid. CONCLUSION: Considering our findings it can be concluded that chloroform soluble fraction of rhizome of D. quercifoliais useful in controlling T. castaneum of stored grain and flour-based products.
Descritores: Praguicidas
Tribolium/efeitos dos fármacos
Controle de Pragas/métodos
Polypodiaceae/química
Rizoma/química
Hidroxibenzoatos/farmacologia
Repelentes de Insetos/farmacologia
-Extratos Vegetais/isolamento & purificação
Extratos Vegetais/química
Clorofórmio
Etanol
Metanol
Alcanos
Hidroxibenzoatos/isolamento & purificação
Dose Letal Mediana
Acetatos
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-886629
Autor: CALEGARI, MATHEUS A; PRASNIEWSKI, ANA CLARA; SILVA, CLEIDIANE DA; SADO, RICARDO Y; MAIA, FABIANA M C; TONIAL, LARISSA M S; OLDONI, TATIANE L C.
Título: Propolis from Southwest of Parana produced by selected bees: Influence of seasonality and food supplementation on antioxidant activity and phenolic profile
Fonte: An. acad. bras. ciênc;89(1):45-55, Jan,-Mar. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Propolis produced by selected bees Apis mellifera were collected from March to June of 2013 and in March of 2015 and analyzed in order to evaluate the influence of climate, colony of origin, and food supplementation of colonies on the content of total phenolic and flavonoid by chromatographic analysis and antioxidant activity by radical scavenging of 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) methods. The Principal Component Analysis (PCA) was carried out with propolis collected in 2013 and two clusters were formed. Propolis produced in the months of March and April showed a higher content of total phenolic compounds (TPC) and antioxidant capacity than those produced in May and June. The results of PCA obtained from samples collected in March of 2013 and 2015 showed two clusters, and propolis collected in 2015 were more bioactive and presented a higher content of TPC. The chromatographic analysis of extracts allowed the identification of phenolic acids p-coumaric, ferulic and caffeic with similar chemical profiles that could be closely related to the botanical origin of propolis. It can be concluded that the season and food supplementation of colonies influenced the chemical composition and the biological activity of samples analysed.
Descritores: Própole/química
Estações do Ano
Abelhas/fisiologia
Suplementos Nutricionais
Hidroxibenzoatos/análise
Antioxidantes/análise
-Valores de Referência
Temperatura
Flavonoides/análise
Ácidos Cafeicos/análise
Análise Multivariada
Reprodutibilidade dos Testes
Cromatografia Líquida de Alta Pressão
Ácidos Cumáricos/análise
Análise de Componente Principal
Indicadores e Reagentes
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-790193
Autor: Mancini, Evelyn Andrea; Pini Prato, Giovan Paolo.
Título: Procedimiento de descontaminación del biofilm para el tratamiento del absceso periodontal agudo y la periimplantitis / The biofilm decontamination approach for the treatment of periodontal abscess and peri-implantitis
Fonte: Rev. Asoc. Odontol. Argent;104(2):79-85, jun. 2016. ilus.
Idioma: es.
Resumo: Objetivo: mostrar el efecto de un nuevo procedimiento de descontaminación del biofilm sobre los abscesos periodontales agudos y la periimplantitis. Casos clínicos: un absceso periodontal agudo y una periimplantitis fueron tratados mediante un material de descontaminación de los tejidos bucales. Este consiste en un concentrado acuoso con una mezcla de ácidos hidroxibencensulfónicos e hidroxymetho-xybencénicos y ácido sulfúrico, que se coloca en las bolsas periodontales y alrededor de los implantes. En ninguno de los casos se utilizaron antibióticos locales ni sistémicos. Todos los casos tratados cicatrizaron rápidamente, sin complicaciones. Los pacientes sintieron una leve molestia durante la aplicación del material, que desapareció completamente en pocos segundos. Conclusión: el procedimiento de desecación del biofilm parecería ser una técnica promisoria para el tratamiento de los abscesos periodontales agudos ylas periimplantitis, con la ventaja adicional de que se evita el uso de antibióticos locales y sistémicos.
Descritores: Abscesso Periodontal/tratamento farmacológico
Biofilmes
Descontaminação/métodos
Peri-Implantite/tratamento farmacológico
-Administração Tópica
Ácidos Sulfônicos/uso terapêutico
Ácidos Sulfúricos/uso terapêutico
Bolsa Periodontal/tratamento farmacológico
Hidroxibenzoatos/uso terapêutico
Limites: Humanos
Feminino
Pessoa de Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: AR29.1 - Biblioteca


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Id: lil-741280
Autor: Yu, Fang-Bo; Li, Xiao-Dan; Ali, Shinawar Waseem; Shan, Sheng-Dao; Luo, Lin-Ping; Guan, Li-Bo.
Título: Further characterization of o-nitrobenzaldehyde degrading bacterium Pseudomonas sp: ONBA-17 and deduction on its metabolic pathway
Fonte: Braz. j. microbiol;45(4):1303-1308, Oct.-Dec. 2014. graf, tab.
Idioma: en.
Projeto: China National Natural Science Foundation. Agricultural and Forestry Carbon Sinks and Ecological Environmental Remediation; . Zhejiang Provincial Natural Science Foundation; . Qingnianbajian Program of Zhejiang Agricultural and Forestry University.
Resumo: A previously reported o-nitrobenzaldehyde (ONBA) degrading bacterium Pseudomonas sp. ONBA-17 was further identified and characterized. Based on results of DNA base composition and DNA-DNA hybridization, the strain was identified as P. putida. Its degradation effect enhanced with increase of inoculum amount and no lag phase was observed. Higher removal rate was achieved under shaking conditions. All tested ONBA with different initial concentrations could be completely degraded within 5 d. In addition, degradative enzyme(s) involved was confirmed as intra-cellular distributed and constitutively expressed. Effects of different compounds on relative activity of degradative enzyme(s) within cell-free extract were also evaluated. Finally, 2-nitrobenzoic acid and 2, 3-dihydroxybenzoic acid were detected as metabolites of ONBA degradation by P. putida ONBA-17, and relevant metabolic pathway was preliminary proposed. This study might help with future research in better understanding of nitroaromatics biodegradation.
Descritores: Benzaldeídos/metabolismo
Redes e Vias Metabólicas
Pseudomonas putida/metabolismo
-Biotransformação
Hidroxibenzoatos/metabolismo
Nitrobenzoatos/metabolismo
Pseudomonas putida/classificação
Pseudomonas putida/genética
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-740227
Autor: Trandafir, Ion; Nour, Violeta; Ionica, Mira Elena.
Título: Antioxidant capacity, phenolic acids and caffeine contents of some commercial coffees available on the Romanian market / Capacidad antioxidante, contenido de ácidos fenólicos y cafeína de algunos tipos de café disponibles en el mercado de Rumania
Fonte: Arch. latinoam. nutr;63(1):87-94, Mar. 2013. ilus, tab.
Idioma: en.
Resumo: In the present study a simple and highly sensitive RP-HPLC method has been established for simultaneous determination of chlorogenic acid, caffeic acid, vanillic acid and caffeine in coffee samples. The method has been applied to eight different coffees available on the Romanian market which were previously analysed concerning the total polyphenols content and antioxidant capacity. Reduction of the DPPH radical was used to determine the antioxidant capacity of the coffee extracts while the total polyphenols content was determined by spectrophotometry (Folin Ciocalteu's method). The total polyphenols content ranged from 1.98 g GAE/100 g to 4.19 g GAE/100 g while the caffeine content ranged from 1.89 g/100 g to 3.05 g/100 g. A large variability was observed in chlorogenic acid content of the investigated coffee samples which ranged between 0.6 and 2.32 g/100 g.

En este trabajo, un método sensible RPHPLC fue desarrollado para la determinación simultánea del ácido clorogénico, del ácido cafeico, del ácido vanílico y de la cafeína en las pruebas de café. El método fue aplicado para analizar ocho tipos de café disponibles en el mercado de Rumania, que fueron analizados también en cuanto al contenido total de polifenoles y la capacidad antioxidante. La reducción del radical DPPH fue utilizada para determinar la capacidad antioxidante de los extractos de café mientras que el contenido total de polifenoles fue determinado por la espectrofotometría (método Folin Ciocalteu). El contenido total de polifenoles se situó entre 1,98 g GAE/100 g y 4.19 g GAE/100 g mientras que el contenido de cafeína se situó entre 1.89 g/100 g y 3.05 g/100 g. Una gran variabilidad se observó con respecto al contenido de ácido clorogénico en las pruebas de café analizadas, contenido que se situó entre 0.6 y 2.32 g/100 g.
Descritores: Antioxidantes/análise
Cafeína/análise
Café/química
Hidroxibenzoatos/análise
-Cromatografia Líquida de Alta Pressão
Sequestradores de Radicais Livres/análise
Romênia
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Id: lil-734615
Autor: Eyduran, Sadiye Peral; Akin, Meleksen; Ercisli, Sezai; Eyduran, Ecevit; Maghradze, David.
Título: Sugars, organic acids, and phenolic compounds of ancient grape cultivars (Vitis vinifera L.) from Igdir province of Eastern Turkey
Fonte: Biol. Res;48:1-8, 2015. tab.
Idioma: en.
Resumo: BACKGROUND: The Eurasian grapevine (Vitis vinifera L.) is the most widely cultivated and economically important horticultural crop in the world. As a one of the origin area, Anatolia played an important role in the diversification and spread of the cultivated form V. vinifera ssp. vinifera cultivars and also the wild form V. vinifera ssp. sylvestris ecotypes. Although several biodiversity studies have been conducted with local cultivars in different regions of Anatolia, no information has been reported so far on the biochemical (organic acids, sugars, phenolic acids, vitamin C) and antioxidant diversity of local historical table V. vinifera cultivars grown in Igdir province. In this work, we studied these traits in nine local table grape cultivars viz. 'Beyaz Kismis' (synonym name of Sultanina or Thompson seedless), 'Askeri', 'El Hakki', 'Kirmizi Kismis', 'Inek Emcegi', 'Hacabas', 'Kerim Gandi', 'Yazen Dayi', and 'Miskali' spread in the Igdir province of Eastern part of Turkey. RESULTS: Variability of all studied parameters is strongly influenced by cultivars (P < 0.01). Among the cultivars investigated, 'Miskali' showed the highest citric acid content (0.959 g/l) while 'Kirmizi Kismis' produced predominant contents in tartaric acid (12.71 g/l). The highest glucose (16.47 g/100 g) and fructose (15.55 g/100 g) contents were provided with 'Beyaz Kismis'. 'Kirmizi Kismis' cultivar had also the highest quercetin (0.55 mg/l), o-coumaric acid (1.90 mg/l), and caffeic acid (2.73 mg/l) content. The highest ferulic acid (0.94 mg/l), and syringic acid (2.00 mg/l) contents were observed with 'Beyaz Kismis' cultivar. The highest antioxidant capacity was obtained as 9.09 μmol TE g-1 from 'Inek Emcegi' in TEAC (Trolox equivalent Antioxidant Capacity) assay. 'Hacabas' cultivar had the highest vitamin C content of 35.74 mg/100 g. CONCLUSIONS: Present results illustrated that the historical table grape cultivars grown in Igdir province of Eastern part of Turkey contained diverse and valuable sugars, organic acids, phenolic acids, Vitamin C values and demonstrated important antioxidant capacity for human health benefits. Further preservation and use of this gene pool will be helpful to avoid genetic erosion and to promote continued agriculture in the region.
Descritores: Ácido Cítrico/análise
Carboidratos da Dieta/análise
Frutas/química
Polifenóis/análise
Vitis/química
-Ácidos/análise
Antioxidantes/análise
Ácido Ascórbico/análise
Ácidos Cafeicos/análise
Ácidos Cumáricos/análise
Produtos Agrícolas/química
Produtos Agrícolas/classificação
Ácido Gálico/análise
Ácido Gálico/análogos & derivados
Hidroxibenzoatos/análise
Turquia
Tartaratos/análise
Vitis/classificação
Responsável: CL1.1 - Biblioteca Central


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Id: lil-577205
Autor: Hyogo, Akiyo; Kobayashi, Toshihiro; Garcia del Saz, Eva; Seguchi, Harumichi.
Título: Antioxidant effects of protocatechuic acid, ferulic acid, and caffeic acid in human neutrophils using a fluorescent substance / Efectos antioxidantes del ácido protocatéquico, ácido ferúlico y el ácido caféico usando una sustancia fluorescente en los neutrófilos humanos
Fonte: Int. j. morphol;28(3):911-920, Sept. 2010. ilus.
Idioma: en.
Projeto: Japan Society for the Promotion of Science.
Resumo: Human neutrophils stimulated by phorbol myristate acetate (PMA), an activator of protein kinase C, produce active oxygen by NADPH oxidase in intracellular structures. We added succinimidyl ester of dichlorodihydrofluorescein diacetate (H2DCFDA), which first emits fluorescence when oxidized with active oxygen species, to neutrophils to produce active oxygen, in order to investigate the antioxidant effects of protocatechuic acid, ferulic acid, and caffeic acid which belong to polyphenols and are widely distributed among plants. Particularly, we focused on examining whether these substances capture and eliminate active oxygen inside or outside the neutrophil cytoplasm and whether these substances inhibit NADPH oxidase. Fluorescence microscopy demonstrated that fluorescence-positive intracellular structures were decreased in neutrophils when stimulated by PMA and exposed to an antioxidant. Quantitative measurement by flow cytometry revealed that the fluorescence intensities in neutrophils, exposed to protocatechuic acid, ferulic acid, or caffeic acid, were decreased by 62.9 percent, 71.4 percent, and 86.1 percent, respectively, as compared with those stimulated by PMA but not exposed to an antioxidant. Judging from fluorescence microscopy and dot blots by flow cytometry, these antioxidants had no effects on neutrophil morphology. On the other hand, the fluorescence intensities of the active oxygen released from neutrophils were decreased by 81.4 percent, 46.7 percent, and 27.4 percent, respectively. Diphenylene iodonium, a specific inhibitor of NADPH oxidase, inhibited the enzyme by 92.1 percent in the PMA-stimulated neutrophils. Protocatechuic acid, ferulic acid, and caffeic acid inhibited the enzyme by 36.5 percent, 54.6 percent, and 27.4 percent, respectively. These results demonstrate that protocatechuic acid, ferulic acid, and caffeic acid capture and eliminate active oxygen, produced by PMA-stimulated neutrophils, intracellularly and extracellularly. Furthe...

Los neutrófilos humanos estimulados por forbol-miristato-acetato (PMA), un activador de la proteína quinasa C, producen oxígeno activo por la NADPH oxidasa en las estructuras intracelulares. Hemos añadido diacetato de 2', 7-dihidro dicloro fluoresceína (H2DCFDA), que emite fluorescencia cuando se oxida con las especies de oxígeno activo, a neutrófilos para producir oxígeno activo, a fin de investigar el efecto antioxidante del ácido protocatéquico, el ácido ferúlico y el ácido cafeico que pertenecen a polifenoles y se distribuyen ampliamente entre las plantas. Particularmente, nos enfocamos en examinar si estas sustancias capturan y eliminan el oxígeno activo dentro o fuera del citoplasma de neutrófilos y si estas sustancias inhiben la NADPH oxidasa. La microscopia de fluorescencia demostró que las estructuras intracelulares positivas a fluorescencia disminuyeron en los neutrófilos mediante la estimulación de la PMA y exposición a un antioxidante. La medición cuantitativa por citometría de flujo reveló que la intensidad de fluorescencia en los neutrófilos, expuestos al ácido protocatéquico, el ácido ferúlico, o el ácido cafeico, se redujo un 62,9 por ciento, 71,4 por ciento y 86,1 por ciento, respectivamente, en comparación con las estimuladas por PMA pero no expuestas a un antioxidante. A juzgar desde la microscopía de fluorescencia y la citometría de flujo, estos antioxidantes no tuvieron efectos sobre la morfología de los neutrófilos. Por otra parte, la intensidad de fluorescencia del oxígeno activo liberado por los neutrófilos se redujeron un 81,4 por ciento, 46,7 por ciento y 27,4 por ciento, respectivamente. El DPI (difenileno-iodonio), un inhibidor específico de la NADPH oxidasa, inhibió a la enzima en el 92,1 por ciento en los neutrófilos estimulados por PMA. El ácido protocatéquico, el ácido ferúlico y el ácido caféico inhiben la enzima en un 36,5 por ciento, 54,6 por ciento y 27,4 por ciento, respectivamente. Estos resultados demuestran...
Descritores: Antioxidantes/farmacologia
Fenóis/farmacologia
Neutrófilos
-Ácidos Cafeicos/farmacologia
Ácidos Cumáricos/farmacologia
Citometria de Fluxo
Fluoresceína
Hidroxibenzoatos
Microscopia de Fluorescência
NADPH Oxidases/antagonistas & inibidores
Acetato de Tetradecanoilforbol
Responsável: CL1.1 - Biblioteca Central


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Id: lil-482081
Autor: Brinkrolf, K; Brune, I; Tauch, A.
Título: Transcriptional regulation of catabolic pathways for aromatic compounds in Corynebacterium glutamicum
Fonte: Genet. mol. res. (Online);5(4):773-789, 2006.
Idioma: en.
Resumo: Corynebacterium glutamicum is a gram-positive soil microorganism able to utilize a large variety of aromatic compounds as the sole carbon source. The corresponding catabolic routes are associated with multiple ring-fission dioxygenases and among other channeling reactions, include the gentisate pathway, the protocatechuate and catechol branches of the beta-ketoadipate pathway and two potential hydroxyquinol pathways. Genes encoding the enzymatic machinery for the bioconversion of aromatic compounds are organized in several clusters in the C. glutamicum genome. Expression of the gene clusters is under specific transcriptional control, apparently including eight DNA-binding proteins belonging to the AraC, IclR, LuxR, PadR, and TetR families of transcriptional regulators. Expression of the gentisate pathway involved in the utilization of 3-hydroxybenzoate and gentisate is positively regulated by an IclR-type activator. The metabolic channeling of ferulate, vanillin and vanillate into the protocatechuate branch of the beta-ketoadipate pathway is controlled by a PadR-like repressor. Regulatory proteins of the IclR and LuxR families participate in transcriptional regulation of the branches of the beta-ketoadipate pathway that are involved in the utilization of benzoate, 4-hydroxybenzoate and protocatechuate. The channeling of phenol into this pathway may be under positive transcriptional control by an AraC-type activator. One of the potential hydroxyquinol pathways of C. glutamicum is apparently repressed by a TetR-type regulator. This global analysis revealed that transcriptional regulation of aromatic compound utilization is mainly controlled by single regulatory proteins sensing the presence of aromatic compounds, thus representing single input motifs within the transcriptional regulatory network of C. glutamicum.
Descritores: Corynebacterium glutamicum/genética
Corynebacterium glutamicum/metabolismo
Hidroxibenzoatos
Hidrocarbonetos Aromáticos/metabolismo
Regulação Bacteriana da Expressão Gênica/genética
Transcrição Genética/genética
-Biodegradação Ambiental
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME



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