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Id: lil-790193
Autor: Mancini, Evelyn Andrea; Pini Prato, Giovan Paolo.
Título: Procedimiento de descontaminación del biofilm para el tratamiento del absceso periodontal agudo y la periimplantitis / The biofilm decontamination approach for the treatment of periodontal abscess and peri-implantitis
Fonte: Rev. Asoc. Odontol. Argent;104(2):79-85, jun. 2016. ilus.
Idioma: es.
Resumo: Objetivo: mostrar el efecto de un nuevo procedimiento de descontaminación del biofilm sobre los abscesos periodontales agudos y la periimplantitis. Casos clínicos: un absceso periodontal agudo y una periimplantitis fueron tratados mediante un material de descontaminación de los tejidos bucales. Este consiste en un concentrado acuoso con una mezcla de ácidos hidroxibencensulfónicos e hidroxymetho-xybencénicos y ácido sulfúrico, que se coloca en las bolsas periodontales y alrededor de los implantes. En ninguno de los casos se utilizaron antibióticos locales ni sistémicos. Todos los casos tratados cicatrizaron rápidamente, sin complicaciones. Los pacientes sintieron una leve molestia durante la aplicación del material, que desapareció completamente en pocos segundos. Conclusión: el procedimiento de desecación del biofilm parecería ser una técnica promisoria para el tratamiento de los abscesos periodontales agudos ylas periimplantitis, con la ventaja adicional de que se evita el uso de antibióticos locales y sistémicos.
Descritores: Abscesso Periodontal/tratamento farmacológico
Biofilmes
Descontaminação/métodos
Peri-Implantite/tratamento farmacológico
-Administração Tópica
Ácidos Sulfônicos/uso terapêutico
Ácidos Sulfúricos/uso terapêutico
Bolsa Periodontal/tratamento farmacológico
Hidroxibenzoatos/uso terapêutico
Limites: Seres Humanos
Feminino
Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: AR29.1 - Biblioteca


  2 / 13 LILACS  
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Texto completo SciELO Brasil
Texto completo
Id: lil-741280
Autor: Yu, Fang-Bo; Li, Xiao-Dan; Ali, Shinawar Waseem; Shan, Sheng-Dao; Luo, Lin-Ping; Guan, Li-Bo.
Título: Further characterization of o-nitrobenzaldehyde degrading bacterium Pseudomonas sp: ONBA-17 and deduction on its metabolic pathway
Fonte: Braz. j. microbiol;45(4):1303-1308, Oct.-Dec. 2014. graf, tab.
Idioma: en.
Projeto: China National Natural Science Foundation. Agricultural and Forestry Carbon Sinks and Ecological Environmental Remediation; . Zhejiang Provincial Natural Science Foundation; . Qingnianbajian Program of Zhejiang Agricultural and Forestry University.
Resumo: A previously reported o-nitrobenzaldehyde (ONBA) degrading bacterium Pseudomonas sp. ONBA-17 was further identified and characterized. Based on results of DNA base composition and DNA-DNA hybridization, the strain was identified as P. putida. Its degradation effect enhanced with increase of inoculum amount and no lag phase was observed. Higher removal rate was achieved under shaking conditions. All tested ONBA with different initial concentrations could be completely degraded within 5 d. In addition, degradative enzyme(s) involved was confirmed as intra-cellular distributed and constitutively expressed. Effects of different compounds on relative activity of degradative enzyme(s) within cell-free extract were also evaluated. Finally, 2-nitrobenzoic acid and 2, 3-dihydroxybenzoic acid were detected as metabolites of ONBA degradation by P. putida ONBA-17, and relevant metabolic pathway was preliminary proposed. This study might help with future research in better understanding of nitroaromatics biodegradation.
Descritores: Benzaldeídos/metabolismo
Redes e Vias Metabólicas
Pseudomonas putida/metabolismo
-Biotransformação
Hidroxibenzoatos/metabolismo
Nitrobenzoatos/metabolismo
Pseudomonas putida/classificação
Pseudomonas putida/genética
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  3 / 13 LILACS  
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Texto completo SciELO Venezuela
Texto completo
Id: lil-740227
Autor: Trandafir, Ion; Nour, Violeta; Ionica, Mira Elena.
Título: Antioxidant capacity, phenolic acids and caffeine contents of some commercial coffees available on the Romanian market / Capacidad antioxidante, contenido de ácidos fenólicos y cafeína de algunos tipos de café disponibles en el mercado de Rumania
Fonte: Arch. latinoam. nutr;63(1):87-94, Mar. 2013. ilus, tab.
Idioma: en.
Resumo: In the present study a simple and highly sensitive RP-HPLC method has been established for simultaneous determination of chlorogenic acid, caffeic acid, vanillic acid and caffeine in coffee samples. The method has been applied to eight different coffees available on the Romanian market which were previously analysed concerning the total polyphenols content and antioxidant capacity. Reduction of the DPPH radical was used to determine the antioxidant capacity of the coffee extracts while the total polyphenols content was determined by spectrophotometry (Folin Ciocalteu's method). The total polyphenols content ranged from 1.98 g GAE/100 g to 4.19 g GAE/100 g while the caffeine content ranged from 1.89 g/100 g to 3.05 g/100 g. A large variability was observed in chlorogenic acid content of the investigated coffee samples which ranged between 0.6 and 2.32 g/100 g.

En este trabajo, un método sensible RPHPLC fue desarrollado para la determinación simultánea del ácido clorogénico, del ácido cafeico, del ácido vanílico y de la cafeína en las pruebas de café. El método fue aplicado para analizar ocho tipos de café disponibles en el mercado de Rumania, que fueron analizados también en cuanto al contenido total de polifenoles y la capacidad antioxidante. La reducción del radical DPPH fue utilizada para determinar la capacidad antioxidante de los extractos de café mientras que el contenido total de polifenoles fue determinado por la espectrofotometría (método Folin Ciocalteu). El contenido total de polifenoles se situó entre 1,98 g GAE/100 g y 4.19 g GAE/100 g mientras que el contenido de cafeína se situó entre 1.89 g/100 g y 3.05 g/100 g. Una gran variabilidad se observó con respecto al contenido de ácido clorogénico en las pruebas de café analizadas, contenido que se situó entre 0.6 y 2.32 g/100 g.
Descritores: Antioxidantes/análise
Cafeína/análise
Café/química
Hidroxibenzoatos/análise
-Cromatografia Líquida de Alta Pressão
Depuradores de Radicais Livres/análise
Romênia
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Texto completo SciELO Chile
Texto completo
Id: lil-734615
Autor: Eyduran, Sadiye Peral; Akin, Meleksen; Ercisli, Sezai; Eyduran, Ecevit; Maghradze, David.
Título: Sugars, organic acids, and phenolic compounds of ancient grape cultivars (Vitis vinifera L.) from Igdir province of Eastern Turkey
Fonte: Biol. Res;48:1-8, 2015. tab.
Idioma: en.
Resumo: BACKGROUND: The Eurasian grapevine (Vitis vinifera L.) is the most widely cultivated and economically important horticultural crop in the world. As a one of the origin area, Anatolia played an important role in the diversification and spread of the cultivated form V. vinifera ssp. vinifera cultivars and also the wild form V. vinifera ssp. sylvestris ecotypes. Although several biodiversity studies have been conducted with local cultivars in different regions of Anatolia, no information has been reported so far on the biochemical (organic acids, sugars, phenolic acids, vitamin C) and antioxidant diversity of local historical table V. vinifera cultivars grown in Igdir province. In this work, we studied these traits in nine local table grape cultivars viz. 'Beyaz Kismis' (synonym name of Sultanina or Thompson seedless), 'Askeri', 'El Hakki', 'Kirmizi Kismis', 'Inek Emcegi', 'Hacabas', 'Kerim Gandi', 'Yazen Dayi', and 'Miskali' spread in the Igdir province of Eastern part of Turkey. RESULTS: Variability of all studied parameters is strongly influenced by cultivars (P < 0.01). Among the cultivars investigated, 'Miskali' showed the highest citric acid content (0.959 g/l) while 'Kirmizi Kismis' produced predominant contents in tartaric acid (12.71 g/l). The highest glucose (16.47 g/100 g) and fructose (15.55 g/100 g) contents were provided with 'Beyaz Kismis'. 'Kirmizi Kismis' cultivar had also the highest quercetin (0.55 mg/l), o-coumaric acid (1.90 mg/l), and caffeic acid (2.73 mg/l) content. The highest ferulic acid (0.94 mg/l), and syringic acid (2.00 mg/l) contents were observed with 'Beyaz Kismis' cultivar. The highest antioxidant capacity was obtained as 9.09 μmol TE g-1 from 'Inek Emcegi' in TEAC (Trolox equivalent Antioxidant Capacity) assay. 'Hacabas' cultivar had the highest vitamin C content of 35.74 mg/100 g. CONCLUSIONS: Present results illustrated that the historical table grape cultivars grown in Igdir province of Eastern part of Turkey contained diverse and valuable sugars, organic acids, phenolic acids, Vitamin C values and demonstrated important antioxidant capacity for human health benefits. Further preservation and use of this gene pool will be helpful to avoid genetic erosion and to promote continued agriculture in the region.
Descritores: Ácido Cítrico/análise
Carboidratos da Dieta/análise
Frutas/química
Polifenóis/análise
Vitis/química
-Ácidos/análise
Antioxidantes/análise
Ácido Ascórbico/análise
Ácidos Cafeicos/análise
Ácidos Cumáricos/análise
Produtos Agrícolas/química
Produtos Agrícolas/classificação
Ácido Gálico/análise
Ácido Gálico/análogos & derivados
Hidroxibenzoatos/análise
Turquia
Tartaratos/análise
Vitis/classificação
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Chile
Texto completo
Id: lil-577205
Autor: Hyogo, Akiyo; Kobayashi, Toshihiro; Garcia del Saz, Eva; Seguchi, Harumichi.
Título: Antioxidant effects of protocatechuic acid, ferulic acid, and caffeic acid in human neutrophils using a fluorescent substance / Efectos antioxidantes del ácido protocatéquico, ácido ferúlico y el ácido caféico usando una sustancia fluorescente en los neutrófilos humanos
Fonte: Int. j. morphol;28(3):911-920, Sept. 2010. ilus.
Idioma: en.
Projeto: Japan Society for the Promotion of Science.
Resumo: Human neutrophils stimulated by phorbol myristate acetate (PMA), an activator of protein kinase C, produce active oxygen by NADPH oxidase in intracellular structures. We added succinimidyl ester of dichlorodihydrofluorescein diacetate (H2DCFDA), which first emits fluorescence when oxidized with active oxygen species, to neutrophils to produce active oxygen, in order to investigate the antioxidant effects of protocatechuic acid, ferulic acid, and caffeic acid which belong to polyphenols and are widely distributed among plants. Particularly, we focused on examining whether these substances capture and eliminate active oxygen inside or outside the neutrophil cytoplasm and whether these substances inhibit NADPH oxidase. Fluorescence microscopy demonstrated that fluorescence-positive intracellular structures were decreased in neutrophils when stimulated by PMA and exposed to an antioxidant. Quantitative measurement by flow cytometry revealed that the fluorescence intensities in neutrophils, exposed to protocatechuic acid, ferulic acid, or caffeic acid, were decreased by 62.9 percent, 71.4 percent, and 86.1 percent, respectively, as compared with those stimulated by PMA but not exposed to an antioxidant. Judging from fluorescence microscopy and dot blots by flow cytometry, these antioxidants had no effects on neutrophil morphology. On the other hand, the fluorescence intensities of the active oxygen released from neutrophils were decreased by 81.4 percent, 46.7 percent, and 27.4 percent, respectively. Diphenylene iodonium, a specific inhibitor of NADPH oxidase, inhibited the enzyme by 92.1 percent in the PMA-stimulated neutrophils. Protocatechuic acid, ferulic acid, and caffeic acid inhibited the enzyme by 36.5 percent, 54.6 percent, and 27.4 percent, respectively. These results demonstrate that protocatechuic acid, ferulic acid, and caffeic acid capture and eliminate active oxygen, produced by PMA-stimulated neutrophils, intracellularly and extracellularly. Furthe...

Los neutrófilos humanos estimulados por forbol-miristato-acetato (PMA), un activador de la proteína quinasa C, producen oxígeno activo por la NADPH oxidasa en las estructuras intracelulares. Hemos añadido diacetato de 2', 7-dihidro dicloro fluoresceína (H2DCFDA), que emite fluorescencia cuando se oxida con las especies de oxígeno activo, a neutrófilos para producir oxígeno activo, a fin de investigar el efecto antioxidante del ácido protocatéquico, el ácido ferúlico y el ácido cafeico que pertenecen a polifenoles y se distribuyen ampliamente entre las plantas. Particularmente, nos enfocamos en examinar si estas sustancias capturan y eliminan el oxígeno activo dentro o fuera del citoplasma de neutrófilos y si estas sustancias inhiben la NADPH oxidasa. La microscopia de fluorescencia demostró que las estructuras intracelulares positivas a fluorescencia disminuyeron en los neutrófilos mediante la estimulación de la PMA y exposición a un antioxidante. La medición cuantitativa por citometría de flujo reveló que la intensidad de fluorescencia en los neutrófilos, expuestos al ácido protocatéquico, el ácido ferúlico, o el ácido cafeico, se redujo un 62,9 por ciento, 71,4 por ciento y 86,1 por ciento, respectivamente, en comparación con las estimuladas por PMA pero no expuestas a un antioxidante. A juzgar desde la microscopía de fluorescencia y la citometría de flujo, estos antioxidantes no tuvieron efectos sobre la morfología de los neutrófilos. Por otra parte, la intensidad de fluorescencia del oxígeno activo liberado por los neutrófilos se redujeron un 81,4 por ciento, 46,7 por ciento y 27,4 por ciento, respectivamente. El DPI (difenileno-iodonio), un inhibidor específico de la NADPH oxidasa, inhibió a la enzima en el 92,1 por ciento en los neutrófilos estimulados por PMA. El ácido protocatéquico, el ácido ferúlico y el ácido caféico inhiben la enzima en un 36,5 por ciento, 54,6 por ciento y 27,4 por ciento, respectivamente. Estos resultados demuestran...
Descritores: Antioxidantes/farmacologia
Fenóis/farmacologia
Neutrófilos
-Ácidos Cafeicos/farmacologia
Ácidos Cumáricos/farmacologia
Citometria de Fluxo
Fluoresceína
Hidroxibenzoatos
Microscopia de Fluorescência
NADPH Oxidases/antagonistas & inibidores
Acetato de Tetradecanoilforbol
Responsável: CL1.1 - Biblioteca Central


  6 / 13 LILACS  
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Id: lil-482081
Autor: Brinkrolf, K; Brune, I; Tauch, A.
Título: Transcriptional regulation of catabolic pathways for aromatic compounds in Corynebacterium glutamicum
Fonte: Genet. mol. res. (Online);5(4):773-789, 2006.
Idioma: en.
Resumo: Corynebacterium glutamicum is a gram-positive soil microorganism able to utilize a large variety of aromatic compounds as the sole carbon source. The corresponding catabolic routes are associated with multiple ring-fission dioxygenases and among other channeling reactions, include the gentisate pathway, the protocatechuate and catechol branches of the beta-ketoadipate pathway and two potential hydroxyquinol pathways. Genes encoding the enzymatic machinery for the bioconversion of aromatic compounds are organized in several clusters in the C. glutamicum genome. Expression of the gene clusters is under specific transcriptional control, apparently including eight DNA-binding proteins belonging to the AraC, IclR, LuxR, PadR, and TetR families of transcriptional regulators. Expression of the gentisate pathway involved in the utilization of 3-hydroxybenzoate and gentisate is positively regulated by an IclR-type activator. The metabolic channeling of ferulate, vanillin and vanillate into the protocatechuate branch of the beta-ketoadipate pathway is controlled by a PadR-like repressor. Regulatory proteins of the IclR and LuxR families participate in transcriptional regulation of the branches of the beta-ketoadipate pathway that are involved in the utilization of benzoate, 4-hydroxybenzoate and protocatechuate. The channeling of phenol into this pathway may be under positive transcriptional control by an AraC-type activator. One of the potential hydroxyquinol pathways of C. glutamicum is apparently repressed by a TetR-type regulator. This global analysis revealed that transcriptional regulation of aromatic compound utilization is mainly controlled by single regulatory proteins sensing the presence of aromatic compounds, thus representing single input motifs within the transcriptional regulatory network of C. glutamicum.
Descritores: Corynebacterium glutamicum/genética
Corynebacterium glutamicum/metabolismo
Hidroxibenzoatos
Hidrocarbonetos Aromáticos/metabolismo
Regulação Bacteriana da Expressão Gênica/genética
Transcrição Genética/genética
-Biodegradação Ambiental
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


  7 / 13 LILACS  
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Texto completo SciELO Brasil
Agra, Maria de Fátima
Texto completo
Id: lil-480837
Autor: Tomaz, Anna Cláudia de A; Nogueira, Raquel Bezerra S. S; Pinto, Danielle Serafim; Agra, Maria de Fátima; Souza, Maria de Fátima V. de; Da-Cunha, Emídio V. Leitão.
Título: Chemical constiuents from Richardia grandiflora (Cham. & Schltdl.) Steud. (Rubiaceae) / Constituintes químicos de Richardia grandiflora (Cham. & Schltdl.) Steud. (Rubiaceae)
Fonte: Rev. bras. farmacogn = Braz. j. pharmacogn.;18(1):47-52, jan.-mar. 2008. ilus, tab.
Idioma: en.
Resumo: Amongst the different forms of therapy to prevent and cure illnesses, plants have been, undoubtedly, the most utilized ones since the beginning of mankind. Brazil has a great diversity on plants that possess non-researched medicinal potential and are promising sources of therapeutic and pharmacological innovations. The Rubiaceae family is considered the biggest one of the order Gentianales, presenting around 637 genera and 10,700 species. Richardia grandiflora (Cham. & Schltdl.) Steud., known popularly as "ervanço", "poaia" or "ipeca-mirim", has ethnopharmacological indications to use as decoction against hemorrhoids and as vermifuge. Aiming at contributing to the chemotaxonomic study of the family Rubiaceae and considering the absence of data in literature about the chemical constitution of the species Richardia grandiflora, the latter was submitted to a phytochemical study to isolate its chemical constituents, through usual chromatographic methods, and after identifying them by means of spectroscopic methods such as ÕH and 13C NMR, with the add of two-dimensional techniques, besides comparison with literature data. Five constituents were isolated through this first phytochemical study with R. grandiflora: a mixture of the steroids beta-sitosterol and stigmasterol, o-hydroxy-benzoic acid, m-methoxy-p-hydroxy-benzoic acid and phaeophitin A, all of them isolated for the first time from the genus Richardia.

Dentre as diversas formas de terapia para a prevenção e cura de doenças, as plantas foram, indubitavelmente, as mais amplamente utilizadas desde o início da humanidade. O Brasil tem grande diversidade de plantas com potenciais medicinais, ainda não pesquisados, e que são promissoras fontes de inovações terapêuticas e farmacológicas. A família Rubiaceae, considerada a maior da ordem Gentianales, possui cerca de 637 gêneros e 10.700 espécies. Richardia grandiflora (Cham. & Schltdl.) Steud., conhecida popularmente como ervanço, poaia ou ipeca-mirim, tem indicações etnofarmacológicas para uso contra hemorróidas e como vermífugo na forma de decocto. Visando a contribuir com o estudo quimiotaxonômico da família Rubiaceae e tendo em vista a ausência de dados na literatura acerca da constituição química de Richardia grandiflora, esta foi submetida a um estudo fitoquímico para o isolamento de seus constituintes químicos, através dos métodos cromatográficos usuais, e posterior identificação estrutural dos mesmos, utilizando-se os métodos espectroscópicos de RMN ÕH e 13C uni e bidimensionais, além de comparações com modelos da literatura. Deste estudo pioneiro com R. grandiflora foram isolados e identificados cinco constituintes: uma mistura dos esteróides beta-sitosterol e estigmasterol, o ácido o-hidroxibenzóico, o ácido m-metoxi-p-hidroxi-benzóico e a feofitina A, todos inéditos no gênero Richardia.
Descritores: Estigmasterol/isolamento & purificação
Estigmasterol/química
Feofitinas/isolamento & purificação
Feofitinas/química
Hidroxibenzoatos
Rubiaceae/química
Responsável: BR8.1 - Biblioteca Central


  8 / 13 LILACS  
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Texto completo SciELO Chile
Texto completo
Id: lil-432430
Autor: Hsu, Chin-Yuan.
Título: Antioxidant activity of extract from Polygonum aviculare L
Fonte: Biol. Res;39(2):281-288, 2006. ilus, tab.
Idioma: en.
Projeto: National Science Council.
Resumo: Free radicals induce numerous diseases by lipid peroxidation, protein peroxidation, and DNA damage. It has been reported that numerous plant extracts have antioxidant activities to scavenge free radicals. Whether Polygonum aviculare L. (Polygonaceae) has antioxidant activity is unknown. In this study, dried Polygonum aviculare L. was extracted by ethanol, and the extract was lyophilized. The antioxidant activities of extract powder were examined by free radical scavenging assays, superoxide radical scavenging assays, lipid peroxidation assays and hydroxyl radical-induced DNA strand scission assays. The results show that the IC50 value of Polygonum aviculare L. extract is 50 µg/ml in free radical scavenging assays, 0.8 µg/ml in superoxide radical scavenging assays, and 15 µg/ml in lipid peroxidation assays, respectively. Furthermore, Polygonum aviculare L. extract has DNA protective effect in hydroxyl radical-induced DNA strand scission assays. The total phenolics and flavonoid content of extract is 677.4 ± 62.7 µg/g and 112.7 ± 13 µg/g. The results indicate that Polygonum aviculare L. extract clearly has antioxidant effects.
Descritores: Antioxidantes/farmacologia
Dano ao DNA/efeitos dos fármacos
Depuradores de Radicais Livres/farmacologia
Peroxidação de Lipídeos/efeitos dos fármacos
Polygonum/química
-Flavonoides/análise
Hidroxibenzoatos
INHIBITORY CONCENTRATION ACADEMIES AND INSTITUTES
Camundongos Endogâmicos BALB C
Nitroazul de Tetrazólio/farmacologia
Picratos/farmacologia
Extratos Vegetais/farmacologia
Tiobarbitúricos/farmacologia
Limites: Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME


  9 / 13 LILACS  
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Texto completo SciELO Chile
Texto completo
Id: lil-309738
Autor: Herrig, Vanessa; Ferrarese, Maria de Lourdes L; Suzuki, Letícia S; Rodrigues, João Domingos; Ferrarese-Filho, Osvaldo.
Título: Peroxidase and phenylalanine ammonia-lyase activities, phenolic acid contents, and allelochemicals-inhibited root growth of soybean
Fonte: Biol. Res;35(1):59-66, 2002. tab, graf.
Idioma: en.
Resumo: The influence of the allelochemicals ferulic (FA) and vanillic (VA) acids on peroxidase (POD, EC 1.11.1.7) and phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activities and their relationships with phenolic acid (PhAs) contents and root growth of soybean (Glycine max (L.) Merr.) were examined. Three-day-old seedlings were cultivated in nutrient solution containing FA or VA (0.1 to 1 mM) for 48 h. Both compounds (at 0.5 and 1 mM) decreased root length (RL), fresh weight (FW) and dry weight (DW) and increased PhAs contents. At 0.5 and 1 mM, FA increased soluble POD activity (18% and 47%, respectively) and cell wall (CW)-bound POD activity (61% and 34%), while VA increased soluble POD activity (33% and 17%) but did not affect CW-bound POD activity. At 1 mM, FA increased (82%) while VA reduced (32%) PAL activities. The results are discussed on the basis of the role of these compounds on phenylpropanoid metabolism and root growth and suggest that the effects caused on POD and PAL activities are some of the many mechanisms by which allelochemicals influence plant growth.
Descritores: Ácidos Cumáricos
Hidroxibenzoatos
Peroxidase
Fenilalanina Amônia-Liase
Raízes de Plantas
Feijão de Soja
Ácido Vanílico
-Peroxidase
Fenilalanina Amônia-Liase
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  10 / 13 LILACS  
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Texto completo SciELO Brasil
Calixto, J. B
Texto completo
Id: lil-281620
Autor: Cunha, J. F; Campestrini, F. D; Calixto, J. B; Scremin, A; Paulino, N.
Título: The mechanism of gentisic acid-induced relaxation of the guinea pig isolated trachea: the role of potassium channels and vasoactive intestinal peptide receptors
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;34(3):381-388, Mar. 2001. ilus.
Idioma: en.
Resumo: We examined some of the mechanisms by which the aspirin metabolite and the naturally occurring metabolite gentisic acid induced relaxation of the guinea pig trachea in vitro. In preparations with or without epithelium and contracted by histamine, gentisic acid caused concentration-dependent and reproducible relaxation, with mean EC50 values of 18 æM and Emax of 100 percent (N = 10) or 20 æM and Emax of 92 percent (N = 10), respectively. The relaxation caused by gentisic acid was of slow onset in comparison to that caused by norepinephrine, theophylline or vasoactive intestinal peptide (VIP). The relative rank order of potency was: salbutamol 7.9 > VIP 7.0 > gentisic acid 4.7 > theophylline 3.7. Gentisic acid-induced relaxation was markedly reduced (24 + or - 7.0, 43 + or - 3.9 and 78 + or - 5.6 percent) in preparations with elevated potassium concentration in the medium (20, 40 or 80 mM, respectively). Tetraethylammonium (100 æM), a nonselective blocker of the potassium channels, partially inhibited the relaxation response to gentisic acid, while 4-AP (10 æM), a blocker of the voltage potassium channel, inhibited gentisic acid-induced relaxation by 41 + or - 12 percent. Glibenclamide (1 or 3 æM), at a concentration which markedly inhibited the relaxation induced by the opener of ATP-sensitive K+ channels, levcromakalim, had no effect on the relaxation induced by gentisic acid. Charybdotoxin (0.1 or 0.3 æM), a selective blocker of the large-conductance Ca2+-activated K+ channels, caused rightward shifts (6- and 7-fold) of the gentisic acid concentration-relaxation curve. L-N G-nitroarginine (100 æM), a NO synthase inhibitor, had no effect on the relaxant effect of gentisic acid, and caused a slight displacement to the right in the relaxant effect of the gentisic acid curve at 300 æM, while methylene blue (10 or 30 æM) or ODQ (1 æM), the inhibitors of soluble guanylate cyclase, all failed to affect gentisic acid-induced relaxation. D-P-Cl-Phe6,Leu17[VIP] (0.1 æM), a VIP receptor antagonist, significantly inhibited (37 + or - 7 percent) relaxation induced by gentisic acid, whereas CGRP (8-37) (0.1 æM), a CGRP antagonist, only slightly enhanced the action of gentisic acid.
Descritores: Hidroxibenzoatos/farmacologia
Técnicas In Vitro
Relaxamento Muscular/efeitos dos fármacos
Canais de Potássio/fisiologia
Receptores de Peptídeo Intestinal Vasoativo/fisiologia
Traqueia/efeitos dos fármacos
-Epitélio/fisiologia
Cobaias
Músculo Liso/efeitos dos fármacos
Receptores de Peptídeo Relacionado com o Gene de Calcitonina/fisiologia
Limites: Animais
Masculino
Feminino
Cobaias
Responsável: BR1.1 - BIREME



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