Base de dados : LILACS
Pesquisa : D02.241.223.100.300.200.299 [Categoria DeCS]
Referências encontradas : 6 [refinar]
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Id: biblio-1087465
Autor: Sepúlveda, Leonardo; Laredo-Alcalá, Elan; Buenrostro-Figueroa, José Juan; Ascacio-Valdés, Juan Alberto; Genisheva, Zlatina; Aguilar, Cristobal; Teixeira, José.
Título: Ellagic acid production using polyphenols from orange peel waste by submerged fermentation
Fonte: Electron. j. biotechnol;43:1-7, Jan. 2020. tab, graf.
Idioma: en.
Projeto: Portuguese Foundation for Science and Technology (FCT); . BioTecNorte operation; . Zlatina Genisheva wish to thank to FCT.
Resumo: Background: Biotechnological processes are part of modern industry as well as stricter environmental requirements. The need to reduce production costs and pollution demands for alternatives that involve the integral use of agro-industrial waste to produce bioactive compounds. The citrus industry generates large amounts of wastes due to the destruction of the fruits by microorganisms and insects together with the large amounts of orange waste generated during the production of juice and for sale fresh. The aim of this study was used orange wastes rich in polyphenolic compounds can be used as source carbon of Aspergillus fumigatus MUM 1603 to generate high added value compounds, for example, ellagic acid and other molecules of polyphenolic origin through submerged fermentation system. Results: The orange peel waste had a high concentration of polyphenols, 28% being condensed, 27% ellagitannins, 25% flavonoids and 20% gallotannins. The major polyphenolic compounds were catechin, EA and quercetin. The conditions, using an experimental design of central compounds, that allow the production of the maximum concentration of EA (18.68 mg/g) were found to be: temperature 30°C, inoculum 2 × 107 (spores/g) and orange peel polyphenols 6.2 (g/L). Conclusion: The submerged fermentation process is an effective methodology for the biotransformation of molecules present in orange waste to obtain high value-added as ellagic acid that can be used as powerful antioxidants, antibacterial and other applications.
Descritores: Gerenciamento de Resíduos
Citrus sinensis/química
Ácido Elágico
-Aspergillus fumigatus
Resíduos/análise
Flavonoides/análise
Biotecnologia/métodos
Taninos Hidrolisáveis/análise
Fermentação
Polifenóis/análise
Compostos Fitoquímicos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-881491
Autor: Oliveira, Luna Mares Lopes de.
Título: Punica granatum: quantificação de polifenóis de extratos e potencial antifúngico contra Candida albicans / Punica granatum: quantification of extract of polyphenols and potential antifungal against candida albicans.
Fonte: Araçatuba; s.n; 2016. 41 p. ilus, graf, tab.
Idioma: pt.
Tese: Apresentada a Universidade Estadual Paulista "Julio de Mesquita Filho". Faculdade de Odontologia de Araçatuba para obtenção do grau de Doutor.
Resumo: As propriedades antimicrobianas da romã são atribuídas aos compostos fenólicos, mais especificamente aos taninos hidrolisáveis (THs) galotaninos (ácido gálico) e elagitaninos (ácido elágico), substâncias naturais de defesa da planta. Os objetivos deste estudo foram avaliar os níveis de ácido elágico e fenóis totais, rendimento dos métodos de extração e o potencial inibitório sobre Candida albicans de extratos de partes da Punica granatum. Amostras em triplicata de cascas, sementes e folhas desidratadas, trituradas, pulverizadas, foram submetidas a dois métodos de extração: soxhlet tendo como solventes etanol e metanol e percolação tendo como solvente o etanol. Após a obtenção dos extratos, foram determinados os teores de ácido elágico por cromatografia liquida de alta eficiência e fenóis totais expressos em ácido gálico pelo método espectrofotométrico. A atividade antifúngica dos extratos foi avaliada pelo método de difusão em disco, seguindo-se as normas do National Committee for Clinical and Laboratory Standards (NCCLS document M2-A8, 2003a.). As maiores concentrações de fenóis totais e de ácido elágico foram encontradas nas folhas e na casca da romã. Verificou-se maior eficiência de rendimento por meio da percolação. Os resultados de difusão em disco revelaram maior sensibilidade da cepa de C. albicans aos extratos etanólicos da casca por ambos os métodos de extração. O método percolação alcoólica a 70% e 25ºC e a casca da romã apresentaram a maior eficiência de extração e de atividade anti-fúngica contra uma cepa clínica de C. albicans(AU)

The antimicrobial properties of pomegranate are attributed to the phenolic compounds, more specifically the hydrolyzable tannins (THs) galotaninos (gallic acid) and elagitaninos (ellagic acid), natural plant defense substances. The objectives of this study were to evaluate the levels of ellagic acid and total phenols, yield of the extraction methods and the inhibitory potential against Candida albicans of extracts from parts of Punica granatum. Samples of dried, peels, seeds and leaves were used in triplicates and the extracts were obtained by Soxhlet system and percolation using respectively ethanol, methanol and ethanol solvents. After obtaining the extracts, the ellagic acid contents were determined by high performance liquid chromatography (HPLC) and total phenols expressed in gallic acid by the spectrophotometric method. The antimicrobial activity was evaluated by the disc diffusion method, following the standards of the National Committee for Clinical and Laboratory Standards (NCCLS document M2-A8, 2003a.). The highest concentrations of THs were found in leaves and pomegranate peel. Greater yield efficiency was verified by percolation. The results of diffusion on disc revealed higher sensitivity of the C. albicans strain to the ethanolic extracts of peels by both methods of extraction, presenting inhibition halos of approximately 10 mm. The alcoholic percolation method at 70% and 25ºC and the pomegranate peel showed the highest extraction efficiency and antifungal activity against a clinical strain of C. albicans(AU)
Descritores: Candida albicans
Romã (Fruta)
Taninos Hidrolisáveis
-Compostos Fenólicos
Responsável: BR186.1 - Biblioteca Honório Monteiro
BR186.1


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Texto completo SciELO Chile
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Id: lil-657663
Autor: Gonçalves, Heloísa Bressan; Riul, Alana Jacomini; Quiapim, Andréa Carla; Jorge, João Atílio; Guimarães, Luis Henrique Souza.
Título: Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus
Fonte: Electron. j. biotechnol;15(5):4-4, Sept. 2012. ilus, tab.
Idioma: en.
Resumo: Background: Tannases are enzymes that may be used in different industrial sectors as, for example, food and pharmaceutical. They are obtained mainly from microorganisms, as filamentous fungi. However, the diversity of fungi stays poorly explored for tannase production. In this article, Aspergillus ochraceus is presented as a new source of tannase with interesting features for biotechnological applications. Results: Extracellular tannase production was induced when the fungus was cultured in Khanna medium with tannic acid as carbon source. The extracellular tannase was purified 9-fold with 2 percent recovery and a single band corresponding to 85 kDa was observed in SDS-PAGE. The native apparent molecular mass was estimated as 112 kDa. Optima of temperature and pH were 40ºC and 5.0, respectively. The enzyme was fully stable from 40ºC to 60ºC during 1 hr. The activity was enhanced by Mn2+ (33-39 percent) and NH4+ (15 percent). The purified tannase hydrolyzed tannic acid and methyl gallate with Km of 0.76 mM and 0.72 mM, respectively, and Vmax of 0.92 U/mg protein and 0.68 U/mg protein, respectively. The analysis of a partial sequence of the tannase encoding gene showed an open read frame of 567 bp and a sequence of 199 amino acids were predicted. TLC analysis revealed the presence of gallic acid as a tannic acid hydrolysis product. Conclusion: The extracellular tannase produced by A. ochraceus showed distinctive characteristics such as monomeric structure and activation by Mn2+, suggesting a new kind of fungal tannases with biotechnological potential. Further, it was the first time that a partial gene sequence for A. ochraceus tannase was described.
Descritores: Aspergillus ochraceus/enzimologia
Hidrolases de Éster Carboxílico/genética
Hidrolases de Éster Carboxílico/metabolismo
-Eletroforese
Fermentação
Concentração de Íons de Hidrogênio
Taninos Hidrolisáveis
Reação em Cadeia da Polimerase
Temperatura
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: lil-656677
Autor: Jana, Arijit; Maity, Chiranjit; Halder, Suman Kumar; Pati, Bikas Ranjan; Mondal, Keshab Chandra; Mohapatra, Pradeep Kumar Das.
Título: Rapid screening of tannase producing microbes by using natural tannin
Fonte: Braz. j. microbiol;43(3):1080-1083, July-Sept. 2012. ilus, tab.
Idioma: en.
Resumo: Use of natural tannin in the screening of tannase producing microbes is really promising. The present work describes about the possibility and integrity of the newly formulated method over the previously reported methods. Tannin isolated from Terminalia belerica Roxb. (Bahera) was used to differentiate between tanninolytic and nontanninolytic microbes. The method is simple, sensitive and superior for the rapid screening and isolation of tannase-producing microbes.
Descritores: Estruturas Vegetais/enzimologia
Fermentação
Tanacetum parthenium/enzimologia
Taninos Hidrolisáveis/análise
Taninos Hidrolisáveis/isolamento & purificação
-Ativação Enzimática
Hidrólise
Métodos
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Texto completo SciELO Brasil
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Id: lil-582766
Autor: Santos, R. M; Oliveira, M. S; Ferri, P. H; Santos, S. C.
Título: Seasonal variation in the phenol content of Eugenia uniflora L. leaves / Variação sazonal nos teores de fenóis de folhas de Eugenia uniflora L
Fonte: Rev. bras. plantas med;13(1):85-89, 2011. ilus, tab.
Idioma: en.
Resumo: Hydrolysable tannins, total phenols and flavonoids in Eugenia uniflora leaves were monthly analysed for one year. The results were correlated with climate conditions (rainfall, humidity, cloudiness and mean temperature) through chemometric methods. Principal component analysis revealed high levels of hydrolysable tannins in the rainy season, whereas flavonoids were mainly produced in the dry season. These facts suggest that climatic changes may be one of the factors affecting phenol levels in Eugenia uniflora.

Taninos hidrolisáveis, fenóis totais e flavonóides presentes em folhas de Eugenia uniflora foram quantificados mensalmente durante um ano. Os resultados foram correlacionados com as condições climáticas (pluviosidade, umidade, nebulosidade e temperatura média) através de métodos quimiométricos. Análise de componentes principais revelou a ocorrência de altos teores de taninos hidrolisáveis durante a estação de chuvas, enquanto os flavonóides foram produzidos principalmente na estação seca. Estes fatos sugerem que mudanças climáticas podem ser um dos fatores que afetam os níveis de fenóis em Eugenia uniflora.
Descritores: Eugenia/crescimento & desenvolvimento
Fenóis/síntese química
Estações do Ano/efeitos adversos
-Mudança Climática
Taninos Hidrolisáveis
Tipo de Publ: Estudo de Avaliação
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


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Id: lil-571412
Autor: Selwal, Manjit K; Yadav, Anita; Selwal, Krishan K; Aggarwal, N. K; Gupta, Ranjan; Gautam, S. K.
Título: Tannase production by Penicillium atramentosum KM under SSF and its applications in wine clarification and tea cream solubilization
Fonte: Braz. j. microbiol;42(1):374-387, Jan.-Mar. 2011. ilus, tab.
Idioma: en.
Resumo: Tannin acyl hydrolase commonly known as tannase is an industrially important enzyme having a wide range of applications, so there is always a scope for novel tannase with better characteristics. A newly isolated tannase-yielding fungal strain identified as Penicillium atramentosum KM was used for tannase production under solid-state fermentation (SSF) using different agro residues like amla (Phyllanthus emblica), ber (Zyzyphus mauritiana), jamun (Syzygium cumini), Jamoa (Eugenia cuspidate) and keekar (Acacia nilotica) leaves. Among these substrates, maximal extracellular tannase production i.e. 170.75 U/gds and 165.56 U/gds was obtained with jamun and keekar leaves respectively at 28ºC after 96 h. A substrate to distilled water ratio of 1:2 (w/v) was found to be the best for tannase production. Supplementation of sodium nitrate (NaNO3) as nitrogen source had enhanced tannase production both in jamun and keekar leaves. Applications of the enzyme were studied in wine clarification and tea cream solubilization. It resulted in 38.05 percent reduction of tannic acid content in case of jamun wine, 43.59 percent reduction in case of grape wine and 74 percent reduction in the tea extract after 3 h at 35ºC.
Descritores: Ativação Enzimática
Fermentação
Hidrolases/análise
Penicillium/enzimologia
Penicillium/isolamento & purificação
Taninos Hidrolisáveis/análise
Taninos Hidrolisáveis/isolamento & purificação
-Catálise
Métodos
Solubilidade
Técnicas
Tipo de Publ: Relatório Técnico
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica



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