Base de dados : LILACS
Pesquisa : D02.241.400 [Categoria DeCS]
Referências encontradas : 24 [refinar]
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  1 / 24 LILACS  
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Id: biblio-1045997
Autor: Su, Fang; Xu, Huarong; Yang, Na; Liu, Wei; Liu, Jianguo.
Título: Hydrolytic efficiency and isomerization during de-esterification of natural astaxanthin esters by saponification and enzymolysis
Fonte: Electron. j. biotechnol;34:37-42, july. 2018. tab, graf.
Idioma: en.
Projeto: The National Natural Science Foundation of China.
Resumo: Background: Astaxanthin from natural sources is typically esterified with fatty acids; hence, it must be hydrolyzed to remove esters before identification and quantification by conventional HPLC. Alkaline-catalyzed saponification and enzyme-catalyzed enzymolysis are the most commonly used de-esterification methods. However, information on the efficiency and isomerization during de-esterification of natural astaxanthin esters by these two methods remains scarce. Therefore, we conducted two HPLC-based experiments to determine which method is better for hydrolyzing astaxanthin esters. Results: To assess the effect of enzymolysis (0.67 U/mL cholesterol esterase, at 37°C) and saponification (0.021 M NaOH, at 5°C) conditions on free astaxanthin recovery and destruction or structural transformation of astaxanthin, we varied the total treatment time across a range of 195 min. The results showed that enzymolysis and saponification were complete in 60 min and 90 min, respectively. After complete hydrolysis, the maximum free astaxanthin recovery obtained by enzymolysis was 42.6% more than that obtained by saponification. The identification of by-products, semi-astacene and astacene, during the process of saponification also indicated that a more severe degradation of astaxanthin occurred during saponification. Moreover, the composition of astaxanthin isomers during saponification was similar to that of the isomers during enzymolysis between 30 min and 75 min (all-trans:9-cis:13-cis = 21:3:1, approximately) but dramatically changed after 90 min, whereas the composition in the enzymolysis treatment remained relatively stable throughout. Conclusion: Compared with saponification, enzymolysis with cholesterol esterase was recommended as a more accurate method for de-esterification of natural astaxanthin esters for further qualitative and quantitative HPLC analysis.
Descritores: Xantofilas/química
Ésteres/química
-Carotenoides
Xantofilas/metabolismo
Álcalis
Enzimas/metabolismo
Ésteres/metabolismo
Hidrólise
Isomerismo
Responsável: CL1.1 - Biblioteca Central


  2 / 24 LILACS  
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Id: biblio-1224608
Autor: Ungcharoenwiwat, Pakpimol; H-Kittikun, Aran.
Título: Enzymatic synthesis of coconut oil based wax esters by immobilized lipase EQ3 and commercial lipozyme RMIM
Fonte: Electron. j. biotechnol;47:10-16, sept. 2020. graf, tab.
Idioma: en.
Projeto: Institute of Research and Innovation, Walailak University.
Resumo: BACKGROUND: Liquid wax esters are widely used in cosmetic as well as pharmaceutical and other industries. The demand of organic and natural products is increasing nowadays. Coconut oil contains benefit fatty acids and has been mainly used for oil-based and moisturizer products. Liquid wax esters from coconut oil and unsaturated fatty alcohol can be synthesized by enzymatic reaction; and it is interesting for using as an alternative natural ingredient in these industries. RESULTS: Optimal condition for coconut oil based wax ester synthesis by immobilized lipase EQ3 was 10 U of enzyme, temperature at 30°C and molar ratio of coconut oil to oleyl alcohol at 1:3 (mol/mol) (0.33X) dissolved in isooctane for 12 h, while for Lipozyme RM IM optimal condition was 10 U of enzyme, temperature at 45°C and oil/alcohol molar ratio at 1:3 (0.33X) dissolved in isooctane for 3 h. Percentage of wax esters synthesized by both lipases reached more than 88%. Both immobilized lipases catalyzed high yield of wax esters within the 2nd batch; after that, the immobilized lipases showed reduced activity and synthesized b60% of wax esters from the 3rd to 5th batch. The main composition of wax esters was ~48% oleyl laurate with 10% degradation at ~250°C. CONCLUSIONS: The liquid wax ester synthesis by commercial Lipozyme RM IM had higher effect than immobilized lipase EQ3, but both catalysts were stable within 2 batches in the optimum condition. The characteristic properties of wax esters showed potential for use as components in cosmetics and skin care products.
Descritores: Ceras
Ésteres/metabolismo
Óleo de Palmeira/síntese química
Lipase/metabolismo
-Temperatura
Enzimas Imobilizadas
Indústria Cosmética
Responsável: CL1.1 - Biblioteca Central


  3 / 24 LILACS  
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Id: biblio-1178863
Autor: Salcedo Pomari, Sandra Isabel; Carita Calla, Arturo; Álvarez Aliaga, María Teresa; Crespo Melgar, Carla Fabiana.
Título: Producción de ésteres volátiles a través de tecnología de fermentaciones utilizando residuos cítricos / Volatile ester production using citrus waste through fermentation technology
Fonte: Con-ciencia (La Paz);4(2):83-91, nov. 2016.
Idioma: es.
Resumo: La acumulación de residuos generados por las industrias procesadoras de alimentos, entre ellos, los residuos cítricos producidos en el procesamiento de jugos, constituyen una problemática desfavorable para el medio ambiente. En diversos países incluyendo Bolivia no existen gestiones que se apliquen al manejo y aprovechamiento de estos residuos, los cuales representan aproximadamente más del 50 % del total de la fruta, por ello es necesaria una gestión integrada de estos residuos de acuerdo al concepto de reducir, reutilizar y reciclar. En el Área de Biotecnología Microbiana del Instituto de Investigaciones Fármaco Bioquímicas, se estudia una alternativa de manejo y aprovechamiento de estos residuos cítricos, investigando la utilización de los mismos como materia prima para la producción de ésteres volátiles que tienen propiedades biosaborizantes. La conversión de residuos cítricos a biosabores se lleva a cabo mediante procesos biotecnológicos de fermentación de azúcares que involucran el uso de microorganismos como levaduras. Para ello, se plantea el estudio del comportamiento cinético de Cándida maltosa asociado a su capacidad de producir ésteres volátiles, considerando que esta levadura es capaz de formar biopelículas como forma de protección ante compuestos inhibitorios propios de la materia prima.

The accumulation of wastes from food processing industries, among them the citrus residues from the juice-processing industry becomes an issue due to its unfavorable impact for the environment. Several countries including Bolivia do not have alternatives for waste management. The citrus waste accounts 50% of the fruit. Therefore,an integrated procedure under the concept to reduce-reuse-recycling is needed.An alternative for management and reuse of citric waste is planned at the Microbial Biotechnology Area of Instituto de Investigaciones Fármaco Bioquímicas. Moreover, the utilization of citrus waste as feedstock for the production of volatile esters, with flavoring properties, is being researched.The conversion of citrus waste to bioflavors can be carried out by sugars-fermenting yeast through biotechnology. Therefore, studies involving the kinetic behaviour of Candida maltosa associated to volatile ester production are planned. This yeast is able to form biofilm as a protection skill against inhibitory compounds from the feedstock.
Descritores: Resíduos
Ésteres
Fermentação
-Candida
Citrus
Açúcares
Alimentos
Responsável: BO138.1 - Biblioteca Central


  4 / 24 LILACS  
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Id: biblio-1051273
Autor: Martinez-Silveira, Adalgisa; Villarreal, Romina; Garmendia, Gabriela; Rufo, Caterina; Vero, Silvana.
Título: Process conditions for a rapid in situ transesterification for biodiesel production from oleaginous yeasts
Fonte: Electron. j. biotechnol;38:1-9, Mar. 2019. tab, graf.
Idioma: en.
Resumo: BACKGROUND: Microbial oils produced by diverse microorganisms are being considered as alternative sources of triglycerides for biodiesel production. However, the standalone production of biodiesel from microorganisms is not currently economically feasible. In case of yeasts, the use of low-value nutrient sources in microbial production and the implementation of cost-efficient downstream processes could reduce costs and make microbial lipids competitive with other commodity-type oils in biodiesel production. Industrial biodiesel synthesis from oleaginous seeds is currently based on a multistep process. However, a simple process called in situ transesterification (ISTE), which takes place within the biomass without a previous lipid extraction step, is receiving increasing interest. In this work, the optimal conditions for an ISTE process to obtain biodiesel from previously selected oleaginous yeast (Rhodotorula graminis S1/S2) were defined using the response surface methodology (RSM). RESULTS: Using the RSM approach, the optimal conditions for the maximum yield with minimum reaction time included a methanol-to-biomass ratio of 60:1, 0.4 M H2SO4, and incubation at 70°C for 3 h. The optimized in situ process yield was significantly higher (123%) than that obtained with a two-step method in which fatty acids from saponifiable lipids were first extracted and then esterified with methanol. The composition of the fatty acid methyl ester mixture obtained from R. graminis S1/S2 by ISTE met Uruguayan standards for biodiesel. CONCLUSION: The characteristics achieved by the optimized method make microbial oil a potential alternative for biodiesel production from yeast at an industrial scale.
Descritores: Leveduras/metabolismo
Biocombustíveis
-Tempo de Reação
Rhodotorula
Biomassa
Meio Ambiente
Esterificação
Ésteres
Ácidos Graxos
Energia Renovável
Lipídeos
Metilação
Responsável: CL1.1 - Biblioteca Central


  5 / 24 LILACS  
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Texto completo SciELO Brasil
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Id: biblio-886744
Autor: OLIVEIRA, SIMONE S S; BELLO, MURILO L; RODRIGUES, CARLOS R; AZEVEDO, PAULA L DE; RAMOS, MARIA C K V; AQUINO-NETO, FRANCISCO R DE; FIAUX, SORELE B; DIAS, LUIZA R S.
Título: Asymmetric bioreduction of ketoesters derivatives by Kluyveromyces marxianus: influence of molecular structure on the conversion and enantiomeric excess
Fonte: An. acad. bras. ciênc;89(3):1403-1415, July-Sept. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT This study presents the bioreduction of six β-ketoesters by whole cells of Kluyveromyces marxianus and molecular investigation of a series of 13 β-ketoesters by hologram quantitative structure-activity relationship (HQSAR) in order to relate with conversion and enantiomeric excess of β-stereogenic-hydroxyesters obtained by the same methodology. Four of these were obtained as (R)-configuration and two (S)-configuration, among them four compounds exhibited >99% enantiomeric excess. The β-ketoesters series LUMO maps showed that the β-carbon of the ketoester scaffold are exposed to undergo nucleophilic attack, suggesting a more favorable β-carbon side to enzymatic reduction based on adopted molecular conformation at the reaction moment. The HQSAR method was performed on the β-ketoesters derivatives separating them into those provided predominantly (R)- or (S)-β-hydroxyesters. The HQSAR models for both (R)- and (S)-configuration showed high predictive capacity. The HQSAR contribution maps suggest the importance of β-ketoesters scaffold as well as the substituents attached therein to asymmetric reduction, showing a possible influence of the ester group carbonyl position on the molecular conformation in the enzyme catalytic site, exposing a β-carbon side to the bioconversion to (S)- and (R)-enantiomers.
Descritores: Kluyveromyces/metabolismo
Ésteres/química
Cetonas/química
-Oxirredução
Biotransformação
Estrutura Molecular
Responsável: BR1.1 - BIREME


  6 / 24 LILACS  
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Texto completo SciELO Brasil
Texto completo
Id: biblio-886755
Autor: VICTOR, MAURICIO M; DAVID, JORGE M; SAKUKUMA, MARIA C K; COSTA-LOTUFO, LETÍCIA V; MOURA, ANDREA F; ARAÚJO, ANA J.
Título: Terpene Esters from Natural Products: Synthesis and Evaluation of Cytotoxic Activity
Fonte: An. acad. bras. ciênc;89(3):1369-1379, July-Sept. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Natural steroids and triterpenes such as b-sitosterol, stigmasterol, lupeol, ursolic and betulinic acids were transformed into its hexanoic and oleic esters, to evaluate the influence of chemical modification towards the cytotoxic activities against tumor cells. The derivatives were evaluated against five tumor cell lines [OVCAR-8 (ovarian carcinoma); SF-295 (glioblastoma); HCT-116 (colon adenocarcinoma); HL-60 (leukemia); and PC-3 (prostate carcinoma)] and the results showed only betulinic acid hexyl ester exhibits cytotoxic potential activity.
Descritores: Triterpenos/farmacologia
Lamiaceae/química
Triterpenos Pentacíclicos/farmacologia
Fabaceae/química
Antineoplásicos/farmacologia
-Triterpenos/isolamento & purificação
Triterpenos/química
Ensaios de Seleção de Medicamentos Antitumorais
Lamiaceae/classificação
Concentração Inibidora 50
Linhagem Celular Tumoral
Ésteres
Triterpenos Pentacíclicos/isolamento & purificação
Triterpenos Pentacíclicos/química
Fabaceae/classificação
Antineoplásicos/isolamento & purificação
Antineoplásicos/química
Limites: Humanos
Responsável: BR1.1 - BIREME


  7 / 24 LILACS  
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Id: biblio-889170
Autor: Ren, Lei; Fan, Shuanghu; Wang, Junhuan; Ruth, Nahurira; Qiao, Cheng; Jia, Yang; Yan, Yanchun.
Título: Complete genome sequence of a phthalic acid esters degrading Mycobacterium sp. YC-RL4
Fonte: Braz. j. microbiol;48(4):607-609, Oct.-Dec. 2017. tab.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Basic Research Fund of CAAS.
Resumo: ABSTRACT Mycobacterium sp. YC-RL4 is capable of utilizing a broad range of phthalic acid esters (PAEs) as sole source of carbon and energy for growth. The preliminary studies demonstrated its high degrading efficiency and good performance during the bioprocess with environmental samples. Here, we present the complete genome of Mycobacterium sp. YC-RL4, which consists of one circular chromosome (5,801,417 bp) and one plasmid (252,568 bp). The genomic analysis and gene annotation were performed and many potential genes responsible for the biodegradation of PAEs were identified from the genome. These results may advance the investigation of bioremediation of PAEs-contaminated environments by strain YC-RL4.
Descritores: Ácidos Ftálicos/metabolismo
Plastificantes/metabolismo
Genoma Bacteriano
Ésteres/metabolismo
Mycobacterium/metabolismo
-Plasmídeos/genética
Plasmídeos/metabolismo
Microbiologia do Solo
Poluentes do Solo/metabolismo
Biodegradação Ambiental
Mycobacterium/isolamento & purificação
Mycobacterium/classificação
Mycobacterium/genética
Responsável: BR1.1 - BIREME


  8 / 24 LILACS  
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Texto completo
Id: biblio-889154
Autor: Shen, Jian Ying; Liao, Jin Zhi; Guo, Li Li; Su, Rui Fang.
Título: Effects of monosulfuron-ester on metabolic processes of nitrogen-fixing cyanobacteria Anabaena flos-aquae and Anabaena azotica
Fonte: Braz. j. microbiol;48(3):544-550, July-Sept. 2017. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: Abstract Presence of the relatively new sulfonylurea herbicide monosulfuron-ester at 0.03-300 nmol/L affected the growth of two non-target nitrogen-fixing cyanobacteria (Anabaena flos-aquae and Anabaena azotica) and substantially inhibited in vitro Acetolactate synthase activity, with IC50 of 3.3 and 101.3 nmol/L for A. flos-aquae and A. azotica, respectively. Presenting in 30-300 nmol/L, it inhibited protein synthesis of the cyanobacteria with less amino acids produced as its concentration increased. Our findings support the view that monosulfuron-ester toxicity in both nitrogen-fixing cyanobacteria is due to its interference with protein metabolism via inhibition of branch-chain amino acid biosynthesis, and particularly Acetolactate synthase activity.
Descritores: Pirimidinas/toxicidade
Compostos de Sulfonilureia/toxicidade
Anabaena/efeitos dos fármacos
Anabaena/metabolismo
Anabaena flos-aquae/efeitos dos fármacos
Anabaena flos-aquae/metabolismo
Ésteres/toxicidade
Herbicidas/toxicidade
Fixação de Nitrogênio/efeitos dos fármacos
-Anabaena/genética
Anabaena flos-aquae/genética
Aminoácidos/metabolismo
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  9 / 24 LILACS  
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Id: biblio-1021560
Autor: Matsakas, Leonidas; Giannakou, Maria; Vörös, Dimitrij.
Título: Effect of synthetic and natural media on lipid production from Fusarium oxysporum
Fonte: Electron. j. biotechnol;30:95-102, nov. 2017. tab, graf.
Idioma: en.
Resumo: Background: Dependence on fossil resources, for the production of fuels and energy, has resulted in environmental and financial problems, which require our immediate action in order to reverse the situation. Use of renewable sources for the production of fuels and energy is an important alternative with biodiesel remains as one of the promising options. Aim of this work is to evaluate the fungus Fusarium oxysporum for its potentials to accumulate microbial lipids when grown on synthetic media and saccharified sweet sorghum stalks. Results: The effect of different carbon sources, nitrogen sources and C/N ratio on the lipid production was initially examined, which resulted in a lipid concentration of 4.4 g/L, with lipid content of 42.6% w/w. Sweet sorghum stalks were able to support growth and lipid production of the fungus, both as carbon source and as nitrogen source. It was also shown that saccharification of the dried stalks is an important step to increase lipid production. Removal of the remaining stalk solids enabled the lipid production during cultivation in increased initial solids of up to 16 w/w. This resulted in a lipid production of 3.81 g/L. Conclusions: It was demonstrated that F. oxysporum can be used as an efficient oleaginous microorganism, with sweet sorghum serving as an excellent raw material for the cultivation of the fungus. The lipids obtained during this work were also found to have a fatty acid profile with good potentials to be used for biodiesel production.
Descritores: Fusarium/metabolismo
Lipídeos/biossíntese
-Carbono/metabolismo
Biomassa
Recursos Renováveis
Combustíveis
Meios de Cultura
Ésteres
Metabolismo dos Lipídeos
Ácidos Graxos/análise
Biocombustíveis
Fermentação
Fusarium/química
Hidrólise
Lipídeos/análise
Nitrogênio/metabolismo
Responsável: CL1.1 - Biblioteca Central


  10 / 24 LILACS  
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Id: biblio-849475
Autor: Martins, Rodrigo dos Santos.
Título: Busca por oxidantes quirais para a transformação enantiosseletiva de compostos orgânicos de boro / Search for chiral oxidants for the enantioselective transformation of organic boron compounds.
Fonte: São Paulo; s.n; 2017. 201p p. ilus, tab, graf.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Instituto de Química para obtenção do grau de Doutor.
Resumo: Neste trabalho, avaliou-se o potencial do uso de oxidantes quirais em oxidações enantiosseletivas de compostos orgânicos de boro. É de conhecimento geral que compostos orgânicos de boro, especialmente ésteres e ácidos borônicos são facilmente oxidados por hidroperóxidos em meio básico. No entanto, são escassos na literatura exemplos destas reações de modo enantiosseletivo. A fim de realizar as reações mencionadas, sintetizou-se os hidroperóxidos quirais TADOOH ({(4R,5R)-5-[(hidroperoxidifenil)metil]-2,2-dimetil-1,3-dioxolan-4il}difenilmetanol) e o hidroperóxido quiral derivado de carboidrato, 2,3-dideoxi1-O-oxidanil-4,6-di-O-pivaloil-α-D-eritro-hex-2-enopiranose (di-O-PivOOH). Estes compostos apresentaram resultados interessantes na literatura em oxidações enantiosseletiva de sulfetos orgânicos, em epoxidações de alcenos e em oxidações de Baeyer-Villiger. Inicialmente o potencial oxidativo de ambos hidroperóxidos, bem como a seletividade destes, foi avaliado frente a diversos ésteres borônicos, sendo que somente o TADOOH apresentou resultados promissores. (Ver esquema no PDF) Observou-se uma melhor seletividade do TADOOH frente a ésteres borônicos que possuíam grupos carbonílicos em sua estrutura. Ao submeter o ß-boronil-éster, 3-fenil-3-(4,4,5,5-tetrametil-1,3,2-dioxaborolan-2-il)propanoato de etila, à oxidação com o TADOOH em THF utilizando NaOH como base, a -30°C por 1 hora, obteve-se o respectivo álcool com 40% de e.e. Cálculos de DFT para o estado de transição na oxidação dos ésteres borônicos com o TADOOH foram realizados em colaboração com o grupo do Prof. Dr. Ataualpa Albert Carmo Braga. Estes cálculos demonstraram que o estado de transição é estabilizado por uma ligação de hidrogênio não clássica entre o oxigênio da carbonila e umas das ligações C-H dos grupos fenila do TADOOH. Além dos estudos relatados, a reconhecida metodologia de Sharpless na epoxidação assimétrica de alcoóis alílicos foi adaptada para a oxidação enantiosseletiva de ésteres borônicos. Ao trocar o ligante derivado de éster tártarico, normalmente utilizado nas epoxidações de Sharpless, por (-)-efedrina observou-se uma moderada seletividade deste sistema frente ao pinacol l-fenietilboronato. Investigações mais detalhadas demonstraram que a presença do Ti(IV) não era necessária, sendo que a (-)efedrina era a responsável pela ativação e indução quiral nesta reação.

In this work, it was investigated the potential use of chiral oxidants in organic boron compound oxidation. It is known in the literature, that organic boron compounds can be easily oxidized by hydroperoxides. However, an enantioselective approach in literature is scarce. In order to perform these reactions, hydroperoxide TADOOH ({(4R,5R)-5[(hydroperoxydiphenyl)methyl]-2,2-dimethyl-l,3-dioxolan-4-yl}diphenylmethanol) and carbohydrate derived hydroperoxide, 2,3-dideoxy-1-O-oxidanyl-4,6-di-O-pivaloyl-α-D-erythro-hex-2-enopyranose (di-O-PivOOH), have been synthesized. These compounds showed interesting results in several enantioselective oxidations, as like, organic sulfides oxidation, alkenes epoxidation and Baeyer-Villiger oxidations. The oxidative potential of both hydroperoxides, as well as their selectivity, were evaluated against several boronic esters. Only TADOOH has shown promissing results for further studies. (See Scheme on PDF). Boronic esters containing a carbonyl moiety showed better selectivities with TADOOH, for example, the reaction of ß-boronyl-ester, ethyl 3-phenyl-3-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)propanoate, gave the correponding alcohol with 40% e.e. DFT calculations for the transition state in the oxidation of the boronic esters with TADOOH were carried out in collaboration with the group of Prof. Dr. Ataualpa Albert Carmo Braga. These calculations have shown that the transition state is stabilized by a non-classical hydrogen bond between the carbonyl oxygen and one of the C-H bonds of the TADOOH phenyl groups. In addition to the studies, the well-known Sharpless protocol for asymmetric epoxidation of allylic alcohols was adapted in the enantioselective oxidation of boronic esters. By replacing the tartaric ester-derived, commonly used in the Sharpless experiments, for (-)-ephedrine moderate selectivity was observed with pinacol 1-phenylethyl boronate. Further investigations showed that the presence of Ti (IV) was not necessary, and (-)-ephedrine was responsible for the activation and chiral induction in this reaction.
Descritores: Ésteres
Compostos Orgânicos
Oxidantes/síntese química
-Compostos de Boro/química
Cinética
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas



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