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Pesquisa : D02.241.400 [Categoria DeCS]
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Id: biblio-1051273
Autor: Martinez-Silveira, Adalgisa; Villarreal, Romina; Garmendia, Gabriela; Rufo, Caterina; Vero, Silvana.
Título: Process conditions for a rapid in situ transesterification for biodiesel production from oleaginous yeasts
Fonte: Electron. j. biotechnol;38:1-9, Mar. 2019. tab, graf.
Idioma: en.
Resumo: BACKGROUND: Microbial oils produced by diverse microorganisms are being considered as alternative sources of triglycerides for biodiesel production. However, the standalone production of biodiesel from microorganisms is not currently economically feasible. In case of yeasts, the use of low-value nutrient sources in microbial production and the implementation of cost-efficient downstream processes could reduce costs and make microbial lipids competitive with other commodity-type oils in biodiesel production. Industrial biodiesel synthesis from oleaginous seeds is currently based on a multistep process. However, a simple process called in situ transesterification (ISTE), which takes place within the biomass without a previous lipid extraction step, is receiving increasing interest. In this work, the optimal conditions for an ISTE process to obtain biodiesel from previously selected oleaginous yeast (Rhodotorula graminis S1/S2) were defined using the response surface methodology (RSM). RESULTS: Using the RSM approach, the optimal conditions for the maximum yield with minimum reaction time included a methanol-to-biomass ratio of 60:1, 0.4 M H2SO4, and incubation at 70°C for 3 h. The optimized in situ process yield was significantly higher (123%) than that obtained with a two-step method in which fatty acids from saponifiable lipids were first extracted and then esterified with methanol. The composition of the fatty acid methyl ester mixture obtained from R. graminis S1/S2 by ISTE met Uruguayan standards for biodiesel. CONCLUSION: The characteristics achieved by the optimized method make microbial oil a potential alternative for biodiesel production from yeast at an industrial scale.
Descritores: Leveduras/metabolismo
Biocombustíveis
-Tempo de Reação
Rhodotorula
Biomassa
Meio Ambiente
Esterificação
Ésteres
Ácidos Graxos
Energia Renovável
Lipídeos
Metilação
Responsável: CL1.1 - Biblioteca Central


  2 / 21 LILACS  
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Texto completo SciELO Brasil
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Id: biblio-886744
Autor: OLIVEIRA, SIMONE S S; BELLO, MURILO L; RODRIGUES, CARLOS R; AZEVEDO, PAULA L DE; RAMOS, MARIA C K V; AQUINO-NETO, FRANCISCO R DE; FIAUX, SORELE B; DIAS, LUIZA R S.
Título: Asymmetric bioreduction of ketoesters derivatives by Kluyveromyces marxianus: influence of molecular structure on the conversion and enantiomeric excess
Fonte: An. acad. bras. ciênc;89(3):1403-1415, July-Sept. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT This study presents the bioreduction of six β-ketoesters by whole cells of Kluyveromyces marxianus and molecular investigation of a series of 13 β-ketoesters by hologram quantitative structure-activity relationship (HQSAR) in order to relate with conversion and enantiomeric excess of β-stereogenic-hydroxyesters obtained by the same methodology. Four of these were obtained as (R)-configuration and two (S)-configuration, among them four compounds exhibited >99% enantiomeric excess. The β-ketoesters series LUMO maps showed that the β-carbon of the ketoester scaffold are exposed to undergo nucleophilic attack, suggesting a more favorable β-carbon side to enzymatic reduction based on adopted molecular conformation at the reaction moment. The HQSAR method was performed on the β-ketoesters derivatives separating them into those provided predominantly (R)- or (S)-β-hydroxyesters. The HQSAR models for both (R)- and (S)-configuration showed high predictive capacity. The HQSAR contribution maps suggest the importance of β-ketoesters scaffold as well as the substituents attached therein to asymmetric reduction, showing a possible influence of the ester group carbonyl position on the molecular conformation in the enzyme catalytic site, exposing a β-carbon side to the bioconversion to (S)- and (R)-enantiomers.
Descritores: Kluyveromyces/metabolismo
Ésteres/química
Cetonas/química
-Oxirredução
Biotransformação
Estrutura Molecular
Responsável: BR1.1 - BIREME


  3 / 21 LILACS  
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Texto completo SciELO Brasil
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Id: biblio-886755
Autor: VICTOR, MAURICIO M; DAVID, JORGE M; SAKUKUMA, MARIA C K; COSTA-LOTUFO, LETÍCIA V; MOURA, ANDREA F; ARAÚJO, ANA J.
Título: Terpene Esters from Natural Products: Synthesis and Evaluation of Cytotoxic Activity
Fonte: An. acad. bras. ciênc;89(3):1369-1379, July-Sept. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Natural steroids and triterpenes such as b-sitosterol, stigmasterol, lupeol, ursolic and betulinic acids were transformed into its hexanoic and oleic esters, to evaluate the influence of chemical modification towards the cytotoxic activities against tumor cells. The derivatives were evaluated against five tumor cell lines [OVCAR-8 (ovarian carcinoma); SF-295 (glioblastoma); HCT-116 (colon adenocarcinoma); HL-60 (leukemia); and PC-3 (prostate carcinoma)] and the results showed only betulinic acid hexyl ester exhibits cytotoxic potential activity.
Descritores: Triterpenos/farmacologia
Lamiaceae/química
Triterpenos Pentacíclicos/farmacologia
Fabaceae/química
Antineoplásicos/farmacologia
-Triterpenos/isolamento & purificação
Triterpenos/química
Ensaios de Seleção de Medicamentos Antitumorais
Lamiaceae/classificação
Concentração Inibidora 50
Linhagem Celular Tumoral
Ésteres
Triterpenos Pentacíclicos/isolamento & purificação
Triterpenos Pentacíclicos/química
Fabaceae/classificação
Antineoplásicos/isolamento & purificação
Antineoplásicos/química
Limites: Humanos
Responsável: BR1.1 - BIREME


  4 / 21 LILACS  
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Id: biblio-889170
Autor: Ren, Lei; Fan, Shuanghu; Wang, Junhuan; Ruth, Nahurira; Qiao, Cheng; Jia, Yang; Yan, Yanchun.
Título: Complete genome sequence of a phthalic acid esters degrading Mycobacterium sp. YC-RL4
Fonte: Braz. j. microbiol;48(4):607-609, Oct.-Dec. 2017. tab.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Basic Research Fund of CAAS.
Resumo: ABSTRACT Mycobacterium sp. YC-RL4 is capable of utilizing a broad range of phthalic acid esters (PAEs) as sole source of carbon and energy for growth. The preliminary studies demonstrated its high degrading efficiency and good performance during the bioprocess with environmental samples. Here, we present the complete genome of Mycobacterium sp. YC-RL4, which consists of one circular chromosome (5,801,417 bp) and one plasmid (252,568 bp). The genomic analysis and gene annotation were performed and many potential genes responsible for the biodegradation of PAEs were identified from the genome. These results may advance the investigation of bioremediation of PAEs-contaminated environments by strain YC-RL4.
Descritores: Ácidos Ftálicos/metabolismo
Plastificantes/metabolismo
Genoma Bacteriano
Ésteres/metabolismo
Mycobacterium/metabolismo
-Plasmídeos/genética
Plasmídeos/metabolismo
Microbiologia do Solo
Poluentes do Solo/metabolismo
Biodegradação Ambiental
Mycobacterium/isolamento & purificação
Mycobacterium/classificação
Mycobacterium/genética
Responsável: BR1.1 - BIREME


  5 / 21 LILACS  
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Id: biblio-889154
Autor: Shen, Jian Ying; Liao, Jin Zhi; Guo, Li Li; Su, Rui Fang.
Título: Effects of monosulfuron-ester on metabolic processes of nitrogen-fixing cyanobacteria Anabaena flos-aquae and Anabaena azotica
Fonte: Braz. j. microbiol;48(3):544-550, July-Sept. 2017. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: Abstract Presence of the relatively new sulfonylurea herbicide monosulfuron-ester at 0.03-300 nmol/L affected the growth of two non-target nitrogen-fixing cyanobacteria (Anabaena flos-aquae and Anabaena azotica) and substantially inhibited in vitro Acetolactate synthase activity, with IC50 of 3.3 and 101.3 nmol/L for A. flos-aquae and A. azotica, respectively. Presenting in 30-300 nmol/L, it inhibited protein synthesis of the cyanobacteria with less amino acids produced as its concentration increased. Our findings support the view that monosulfuron-ester toxicity in both nitrogen-fixing cyanobacteria is due to its interference with protein metabolism via inhibition of branch-chain amino acid biosynthesis, and particularly Acetolactate synthase activity.
Descritores: Pirimidinas/toxicidade
Compostos de Sulfonilureia/toxicidade
Anabaena/efeitos dos fármacos
Anabaena/metabolismo
Anabaena flos-aquae/efeitos dos fármacos
Anabaena flos-aquae/metabolismo
Ésteres/toxicidade
Herbicidas/toxicidade
Fixação de Nitrogênio/efeitos dos fármacos
-Anabaena/genética
Anabaena flos-aquae/genética
Aminoácidos/metabolismo
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  6 / 21 LILACS  
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Id: biblio-1021560
Autor: Matsakas, Leonidas; Giannakou, Maria; Vörös, Dimitrij.
Título: Effect of synthetic and natural media on lipid production from Fusarium oxysporum
Fonte: Electron. j. biotechnol;30:95-102, nov. 2017. tab, graf.
Idioma: en.
Resumo: Background: Dependence on fossil resources, for the production of fuels and energy, has resulted in environmental and financial problems, which require our immediate action in order to reverse the situation. Use of renewable sources for the production of fuels and energy is an important alternative with biodiesel remains as one of the promising options. Aim of this work is to evaluate the fungus Fusarium oxysporum for its potentials to accumulate microbial lipids when grown on synthetic media and saccharified sweet sorghum stalks. Results: The effect of different carbon sources, nitrogen sources and C/N ratio on the lipid production was initially examined, which resulted in a lipid concentration of 4.4 g/L, with lipid content of 42.6% w/w. Sweet sorghum stalks were able to support growth and lipid production of the fungus, both as carbon source and as nitrogen source. It was also shown that saccharification of the dried stalks is an important step to increase lipid production. Removal of the remaining stalk solids enabled the lipid production during cultivation in increased initial solids of up to 16 w/w. This resulted in a lipid production of 3.81 g/L. Conclusions: It was demonstrated that F. oxysporum can be used as an efficient oleaginous microorganism, with sweet sorghum serving as an excellent raw material for the cultivation of the fungus. The lipids obtained during this work were also found to have a fatty acid profile with good potentials to be used for biodiesel production.
Descritores: Fusarium/metabolismo
Lipídeos/biossíntese
-Carbono/metabolismo
Biomassa
Recursos Renováveis
Combustíveis
Meios de Cultura
Ésteres
Metabolismo dos Lipídeos
Ácidos Graxos/análise
Biocombustíveis
Fermentação
Fusarium/química
Hidrólise
Lipídeos/análise
Nitrogênio/metabolismo
Responsável: CL1.1 - Biblioteca Central


  7 / 21 LILACS  
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Id: biblio-849475
Autor: Martins, Rodrigo dos Santos.
Título: Busca por oxidantes quirais para a transformação enantiosseletiva de compostos orgânicos de boro / Search for chiral oxidants for the enantioselective transformation of organic boron compounds.
Fonte: São Paulo; s.n; 2017. 201p p. ilus, tab, graf.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Instituto de Química para obtenção do grau de Doutor.
Resumo: Neste trabalho, avaliou-se o potencial do uso de oxidantes quirais em oxidações enantiosseletivas de compostos orgânicos de boro. É de conhecimento geral que compostos orgânicos de boro, especialmente ésteres e ácidos borônicos são facilmente oxidados por hidroperóxidos em meio básico. No entanto, são escassos na literatura exemplos destas reações de modo enantiosseletivo. A fim de realizar as reações mencionadas, sintetizou-se os hidroperóxidos quirais TADOOH ({(4R,5R)-5-[(hidroperoxidifenil)metil]-2,2-dimetil-1,3-dioxolan-4il}difenilmetanol) e o hidroperóxido quiral derivado de carboidrato, 2,3-dideoxi1-O-oxidanil-4,6-di-O-pivaloil-α-D-eritro-hex-2-enopiranose (di-O-PivOOH). Estes compostos apresentaram resultados interessantes na literatura em oxidações enantiosseletiva de sulfetos orgânicos, em epoxidações de alcenos e em oxidações de Baeyer-Villiger. Inicialmente o potencial oxidativo de ambos hidroperóxidos, bem como a seletividade destes, foi avaliado frente a diversos ésteres borônicos, sendo que somente o TADOOH apresentou resultados promissores. (Ver esquema no PDF) Observou-se uma melhor seletividade do TADOOH frente a ésteres borônicos que possuíam grupos carbonílicos em sua estrutura. Ao submeter o ß-boronil-éster, 3-fenil-3-(4,4,5,5-tetrametil-1,3,2-dioxaborolan-2-il)propanoato de etila, à oxidação com o TADOOH em THF utilizando NaOH como base, a -30°C por 1 hora, obteve-se o respectivo álcool com 40% de e.e. Cálculos de DFT para o estado de transição na oxidação dos ésteres borônicos com o TADOOH foram realizados em colaboração com o grupo do Prof. Dr. Ataualpa Albert Carmo Braga. Estes cálculos demonstraram que o estado de transição é estabilizado por uma ligação de hidrogênio não clássica entre o oxigênio da carbonila e umas das ligações C-H dos grupos fenila do TADOOH. Além dos estudos relatados, a reconhecida metodologia de Sharpless na epoxidação assimétrica de alcoóis alílicos foi adaptada para a oxidação enantiosseletiva de ésteres borônicos. Ao trocar o ligante derivado de éster tártarico, normalmente utilizado nas epoxidações de Sharpless, por (-)-efedrina observou-se uma moderada seletividade deste sistema frente ao pinacol l-fenietilboronato. Investigações mais detalhadas demonstraram que a presença do Ti(IV) não era necessária, sendo que a (-)efedrina era a responsável pela ativação e indução quiral nesta reação.

In this work, it was investigated the potential use of chiral oxidants in organic boron compound oxidation. It is known in the literature, that organic boron compounds can be easily oxidized by hydroperoxides. However, an enantioselective approach in literature is scarce. In order to perform these reactions, hydroperoxide TADOOH ({(4R,5R)-5[(hydroperoxydiphenyl)methyl]-2,2-dimethyl-l,3-dioxolan-4-yl}diphenylmethanol) and carbohydrate derived hydroperoxide, 2,3-dideoxy-1-O-oxidanyl-4,6-di-O-pivaloyl-α-D-erythro-hex-2-enopyranose (di-O-PivOOH), have been synthesized. These compounds showed interesting results in several enantioselective oxidations, as like, organic sulfides oxidation, alkenes epoxidation and Baeyer-Villiger oxidations. The oxidative potential of both hydroperoxides, as well as their selectivity, were evaluated against several boronic esters. Only TADOOH has shown promissing results for further studies. (See Scheme on PDF). Boronic esters containing a carbonyl moiety showed better selectivities with TADOOH, for example, the reaction of ß-boronyl-ester, ethyl 3-phenyl-3-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)propanoate, gave the correponding alcohol with 40% e.e. DFT calculations for the transition state in the oxidation of the boronic esters with TADOOH were carried out in collaboration with the group of Prof. Dr. Ataualpa Albert Carmo Braga. These calculations have shown that the transition state is stabilized by a non-classical hydrogen bond between the carbonyl oxygen and one of the C-H bonds of the TADOOH phenyl groups. In addition to the studies, the well-known Sharpless protocol for asymmetric epoxidation of allylic alcohols was adapted in the enantioselective oxidation of boronic esters. By replacing the tartaric ester-derived, commonly used in the Sharpless experiments, for (-)-ephedrine moderate selectivity was observed with pinacol 1-phenylethyl boronate. Further investigations showed that the presence of Ti (IV) was not necessary, and (-)-ephedrine was responsible for the activation and chiral induction in this reaction.
Descritores: Ésteres
Compostos Orgânicos
Oxidantes/síntese química
-Compostos de Boro/química
Cinética
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


  8 / 21 LILACS  
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Texto completo SciELO Chile
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Id: lil-764082
Autor: Amigo, Hugo; Bustos, Patricia; Vargas, Claudio; Iglesias, Pablo.
Título: Variación secular de los nacimientos, peso y longitud al nacer: perspectiva local / Secular variation of births, weight and length at birth: Local perspective
Fonte: Rev. chil. pediatr;86(4):257-263, ago. 2015. graf.
Idioma: es.
Projeto: Fondecyt.
Resumo: Objetivo: Analizar la evolución de los nacimientos y medidas antropométricas al nacer entre 1974-2011 en el Hospital de Limache, Región de Valparaíso, Chile. Pacientes y métodos: Se construyeron series de tiempo de nacimientos, peso y longitud al nacer, peso y talla baja al nacer. Se modelaron las tendencias con regresiones multivariadas usándose splines para representar los cambios de tendencia por década. Resultados: La serie comprende 17.574 nacimientos. Hubo un aumento de los nacimientos/año en los 70 (30/año) y disminución de 17 y 22 nacimientos/año en los 80 y 90 (p < 0,001); después, sin tendencia significativa. Los recién nacidos entre 2000-2011 registran 266 g más que los de la década de los 70 (p < 0,001), alcanzando actualmente en promedio 3.530 g. El bajo peso al nacer disminuyó de 8% en los 70 a 1,1% después de 2000. La longitud al nacer incrementó 1 cm en 37 años, con disminución de la talla baja de 7,6% a 2,1% en el periodo estudiado. Conclusión: Los nacimientos en el Hospital de Limache disminuyeron y las medidas antropométricas al nacer mejoraron; sin embargo, hay que considerar los posibles sesgos que distorsionan estas estimaciones.

Objective: To analyse the outcomes of births and anthropometric measurements at birth of children born between 1974 and 2011 at Limache Hospital (Valparaíso, Chile). Patients and method: Times series were constructed of births, weight and length at birth, and low weight and length at birth. The trend was modelled with linear and logistical regressions using splines to represent breaks in the trend by decade. Results: The series includes 17,574 births. There was an increase in births per year in the 1970s (30/year) and declines in them to 17 and 22 births/year in the 1980s and 1990s, respectively (P < .001), with no significant trend thereafter. Newborns from 2000 to 2011 weighed 266 grams more than those in the 1970s (P < .001), and have now reached a mean weight of 3,530 g. Low birthweight fell from 8% in the 1970s to 1.1% after 2000. Birth length increased by 1 cm in the 37 years studied, with a reduction of low birth length from 7.6% to 2.1% during the period. Conclusion: Live births in the Limache Hospital declined, and anthropometric measurements at birth improved in the years analysed. This information is useful in developing interventions, taking into account the possible selection biases that could distort these estimates and their interpretation.
Descritores: Apomorfina/química
Pró-Fármacos/química
-Administração Oral
Sistemas de Liberação de Medicamentos/métodos
Emulsões/química
Ésteres/química
Hidrólise
Lipídeos/química
Extratos Pancreáticos/química
Suínos
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  9 / 21 LILACS  
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Texto completo SciELO Chile
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Id: lil-764081
Autor: Castillo C, Carmen; Santander M, Dolores; Solís F, Fresia.
Título: Nivel de autoconcepto en niños con secuelas de quemaduras: estudio comparativo / Self-concept level in children with burns sequelae: A comparative study
Fonte: Rev. chil. pediatr;86(4):251-256, ago. 2015. tab.
Idioma: es.
Resumo: Introducción: Autoconcepto es el conjunto de ideas y actitudes que se tiene acerca de sí mismo. Nuestro objetivo fue evaluar si existen diferencias en el nivel de autoconcepto de niños de 8-12 años con y sin secuelas de quemaduras, e identificar variables predictoras del autoconcepto en los niños con secuelas. Pacientes y método: Estudio comparativo, transversal de 109 niños con secuelas de quemaduras de 8 a 12 años de edad, con 109 niños sin secuelas de quemaduras, del mismo grupo de edad y nivel socioeconómico. Se utilizó la escala de autoconcepto de Piers-Harris, que entrega medida de autoconcepto general y dimensiones: conductual, estatus intelectual y escolar, apariencia y atributos físicos, ansiedad, popularidad, felicidad y satisfacción. Resultados: No hubo diferencias significativas en el nivel de autoconcepto general ni en sus dimensiones al comparar ambos grupos (p > 0,05). Dentro del grupo con secuelas de quemaduras la variable número de secuelas resultó ser un factor protector para las dimensiones ansiedad, popularidad, felicidad-satisfacción y autoconcepto general. La variable localización surgió como factor de riesgo para la dimensión conductual. Discusión: La ausencia de diferencias en autoconcepto entre niños con secuelas de quemaduras y sin ellas es similar a lo reportado por la literatura. El hallazgo en factores de riesgo y protectores motiva a continuar investigando, incorporando antecedentes premórbidos y familiares.

Introduction: Self-concept is the set of ideas and attitudes that a person has about him/herself. Objective: To evaluate whether there are differences in the level of self-concept in children 8-12 years old with and without burns sequelae. To identify predictive variables of self-concept in children with sequelae. Patients and method: A comparative cross-sectional study of self-concept in 109 children with burns sequelae, from 8 to 12 years old, with 109 children without burns sequelae, and of the same age and socioeconomic status. The Piers-Harris self-concept scale is used, which provides a general measurement of self-concept and behavioural, intellectual and school status, appearance, and physical attributes, anxiety, popularity, happiness and satisfaction dimensions. Results: There were no significant differences in the level of general self-concept or their dimensions (P > .05). In the group with burns sequelae, the protective factor was the variable number of sequels was associated with the dimensions of anxiety, popularity, happiness-satisfaction and general self-concept. The location variable emerged as a risk factor for the behavioural dimension. Discussion: The absence of differences in self-concept between children with burns sequelae and children without them is similar to that reported in the literature. The finding in the risk and protective factors encourages to further research, and perhaps incorporating pre-morbidity and family background.
Descritores: DNA
Compostos de Amônio Quaternário/química
Serina/química
Tensoativos/química
-Amidas/química
Aminas/química
Linhagem Celular Tumoral
Química Farmacêutica/métodos
Ésteres/química
Técnicas de Transferência de Genes
Terapia Genética/métodos
Células HeLa
Lipídeos/química
Transfecção/métodos
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  10 / 21 LILACS  
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Texto completo SciELO Saúde Pública
Texto completo
Id: lil-733291
Autor: Schraiber, Lilia Blima; d'Oliveira, Ana Flávia Pires Lucas.
Título: La perspectiva de género y los profesionales de la salud: apuntes desde la salud colectiva brasileña / The gender perspective and health professionals: notes from the Brazilian collective health field
Fonte: Salud colect;10(3):301-312, sep.-dic. 2014.
Idioma: es.
Resumo: Para analizar la incorporación del género al campo de la salud, consideramos la producción científica, las políticas y programas de salud, además de las prácticas profesionales en el cotidiano de los servicios. Estos distintos planos de abordaje resultan necesarios por las diversas posibilidades que cada uno de ellos presenta ante dicha incorporación. En la producción científica, se identifica el crecimiento de la perspectiva de género, pero con baja inscripción metodológica del concepto; en las políticas y programas de salud, una ausencia de transversalidad y una presencia de inestabilidad temporal; y, en las prácticas profesionales, una incorporación más anclada en saberes prácticos que en la dimensión técnico-científica. El conjunto de las dificultades genera distintas tensiones en el desempeño cotidiano de los profesionales, entre la base científico-tecnológica y la base moral de la intervención.

We examine the incorporation of the gender perspective in the health field, considering scientific production, health policies and programs and everyday professional practices within the health services. These distinct layers are necessary given the different possibilities each presents for the incorporation of gender. In scientific production, we identify increasing inclusion of the gender perspective, but with little methodological use of the concept; in health policies and programs, the incorporation of the gender perspective is not comprehensive and varies temporally; and in professional practices, incorporation is anchored more in practical knowledge than in a technical and scientific basis. In the daily work of health professionals, this set of difficulties generates different tensions regarding the scientific and technological basis and the moral basis for intervention.
Descritores: Frutas/química
Odorantes/análise
Psidium/química
-Cromatografia Gasosa
Costa Rica
Cicloexenos
Ésteres/análise
Cromatografia Gasosa-Espectrometria de Massas
Monoterpenos/análise
Sesquiterpenos
Olfato
Paladar
Terpenos/análise
Volatilização
Limites: Humanos
Responsável: BR1.1 - BIREME



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