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Id: biblio-1282553
Autor: Braz, Maria Cleide Azevedo; Saguchi, André Hayato; Akisue, Eduardo; Lira, Adriana de Oliveira; Paiva, Sidnea Aparecida Freitas; Brugnera Junior, Aldo; Macedo, Mary Caroline Skelton; Michelotto, André Luiz da Costa; Araki, Ângela Toshie.
Título: Comparative in vitro study of intracanal Enterococcus faecalis reduction using photosensitizers in aPDT
Fonte: Braz. j. oral sci;20:e214034, jan.-dez. 2021. ilus.
Idioma: en.
Resumo: Aim: To compare Enterococcus faecalis reduction after antimicrobial photodynamic therapy (aPDT) used with methylene blue, toluidine blue, tannin, and curcumin as photosensitizers, an adjunct to endodontic chemomechanical preparation (CMP) in root canals of human teeth. Methods: A total of 120 single-rooted teeth were divided into 6 groups (n = 20): G1- CMP and 2.5% sodium hypochlorite (NaOCl); G2- CMP and saline solution; G3- CMP, 2.5% NaOCl, and aPDT with 0.005% methylene blue; G4- CMP, 2.5% NaOCl, and aPDT with 0.005% toluidine blue; G5- CMP, 2.5% NaOCl, and aPDT with 0.005% tannin; and G6- CMP, 2.5% NaOCl, and aPDT with 0.005% curcumin. A portable semiconductor laser was used (660 nm, 100 mW, 1.8 J, 180s) in groups 1, 2, 3, 4, 5, and a blue LED light-curing (420-480 nm, 1200 mV/cm2 ) in G6. For all groups, a 5 min pre-irradiation time was applied. Samples were collected before (initial collection), immediately after (intermediate collection) and 7 days after CMP (final collection) for colony-forming unit (CFU) counting. The Kruskal-Wallis test and analysis of variance (ANOVA) were performed (p < 0.05; 95% confidence interval). Results: In between-group comparisons, there was no significant difference observed in the number of CFUs at the initial (p >< 0.001) and final collections (p >< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies.>< 0.05; 95% confidence interval). Results: In between-group comparisons, there was no significant difference observed in the number of CFUs at the initial (p < 0.001) and final collections (p >< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies.>< 0.001) and final collections (p < 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies>< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies
Descritores: Fotoquimioterapia
Taninos
Fármacos Fotossensibilizantes
Curcumina
Endodontia
Responsável: BR218.1 - Biblioteca Carlos Henrique Robertson Liberalli


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Id: biblio-832973
Autor: Marchi, Juliana Pelissari; Tedesco, Luana; Melo, Ailton da Cruz; Frasson, Andressa Caroline; França, Vivian Francielle; Sato, Samantha Wietzikoski; Lovato, Evellyn Claudia Wietzikoski.
Título: Curcuma longa L, o açafrão da terra, e seus benefícios medicinais / Curcuma longa L, earth saffron and its medical benefits
Fonte: Arq. ciências saúde UNIPAR;20(3):189-194, set.-dez. 2016. tab.
Idioma: pt.
Resumo: Plantas medicinais são de grande importância nos estudos para elaboração de fármacos de origem natural. A Curcuma Longa Linn é uma planta pertencente a espécie Zingiberaceae, cujo nome científico é Curcuma longa L. É popularmente conhecida como açafrão, açafrão da terra, gengibre amarela e raiz de sol. Possui aspecto rizomatoso e forte tonalidade amarela. Originária da ásia e cultivada em regiões tropicais e subtropicais, é muito utilizada na culinária e medicina popular. Esta revisão bibliográfica teve por objetivo descrever os principais usos medicinais da Curcuma longa L. É utilizada como tempero, especialmente na região continental da Índia. Sendo consumido principalmente na forma de pó seco, utilizado para coloração alimentícia devido à sua forte coloração amarela, associada aos efeitos terapêuticos e dietéticos Os pigmentos responsáveis por essa coloração forte, pertencem à classe dos diferoluilmetano, representados pela curcumina, se tornando bastante utilizada como corante. Os rizomas da cúrcuma são processados e deles se extraem óleo essencial, curcumina e fécula. Possui diversas atividades farmacológicas, destacando seus principais potenciais terapêuticos, tais como; atividades anti-inflamatória, antiviral, antibactericida, antioxidante, antifúngica, anticarcinogênica, entre outras ações. Estudos indicaram que a curcumina apresenta efeitos neuroprotetores no tratamento da Doença de Alzheimer e de Parkinson, prevenindo a inflamação e o dano oxidativo. A pesquisa permitiu verificar a importância da Curcuma longa L. e seu grande potencial de utilização, contendo, principalmente, efeito anti-inflamatório e provável efeito neuroprotetor com potencial para o desenvolvimento de medicamentos. Diante disso, fazem-se necessários estudos, para avaliar seu potencial como planta medicinal.

Medicinal plants are of great importance in studies for the preparation of naturally occurring drugs. Curcuma Longa Linn is a plant belonging to the Zingiberaceae species, whose scientific name is Curcuma longa L.. It is popularly known as saffron, earth saffron, yellow ginger and sun root, with a rhizomatous aspect and strong yellow hue. It is originally from Asia, cultivated in tropical and subtropical regions, and widely used in cooking and folk medicine. This literature review aimed to describe the main medicinal uses of Curcuma Longa L. It is used as a spice, especially in mainland India. Being mainly consumed as a dry powder, used for food coloring due to its strong yellow color associated with therapeutic and dietary effects. Its pigments are responsible for this strong color, belong to the diferuloylmethane class represented by curcumin, becoming widely used as food coloring. The turmeric rhizomes are processed and essential oil, curcumin and starch are extracted from it. It presents several pharmacological activities, such as anti-inflammatory, antiviral, antibacterial, antioxidant, antifungal and anticarcinogenic activities, among others. Studies have shown that curcumin has neuroprotective effects in the treatment of Alzheimer's disease and Parkinson's disease, preventing inflammation and oxidative damage. Research has shown the importance of Curcuma Longa L. and its great use potential. It presents anti-inflammatory and possible neuroprotective effects with potential for drug development. Therefore, further studies are necessary in order to assess its potential as a medicinal plant.
Descritores: Plantas Medicinais
Curcumina
Curcuma
Antioxidantes
Responsável: BR1532.9 - CEDIC - Coordenadoria de Editoração e Divulgação Científica


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Id: biblio-950770
Autor: Soto-Urquieta, María G; López-Briones, Sergio; Pérez-Vázquez, Victoriano; Saavedra-Molina, Alfredo; González-Hernández, Gloria A; Ramírez-Emiliano, Joel.
Título: Curcumin restores mitochondrial functions and decreases lipid peroxidation in liver and kidneys of diabetic db/db mice
Fonte: Biol. Res;47:1-8, 2014. graf.
Idioma: en.
Resumo: BACKGROUND: Nitrosative and oxidative stress play a key role in obesity and diabetes-related mitochondrial dysfunction. The objective was to investigate the effect of curcumin treatment on state 3 and 4 oxygen consumption, nitric oxide (NO) synthesis, ATPase activity and lipid oxidation in mitochondria isolated from liver and kidneys of diabetic db/db mice. RESULTS: Hyperglycaemia increased oxygen consumption and decreased NO synthesis in liver mitochondria isolated from diabetic mice relative to the control mice. In kidney mitochondria, hyperglycaemia increased state 3 oxygen consumption and thiobarbituric acid-reactive substances (TBARS) levels in diabetic mice relative to control mice. Interestingly, treating db/db mice with curcumin improved or restored these parameters to normal levels; also curcumin increased liver mitochondrial ATPase activity in db/db mice relative to untreated db/db mice. CONCLUSIONS: These findings suggest that hyperglycaemia modifies oxygen consumption rate, NO synthesis and increases TBARS levels in mitochondria from the liver and kidneys of diabetic mice, whereas curcumin may have a protective role against these alterations.
Descritores: Peroxidação de Lipídeos/efeitos dos fármacos
Curcumina/farmacologia
Diabetes Mellitus Tipo 2/dietoterapia
Rim/efeitos dos fármacos
Fígado/efeitos dos fármacos
Mitocôndrias/efeitos dos fármacos
-Consumo de Oxigênio/efeitos dos fármacos
Peso Corporal/efeitos dos fármacos
Mitocôndrias Hepáticas/efeitos dos fármacos
Mitocôndrias Hepáticas/enzimologia
Adenosina Trifosfatases/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Respiração Celular/efeitos dos fármacos
Suplementos Nutricionais
Diabetes Mellitus Tipo 2/complicações
Diabetes Mellitus Tipo 2/fisiopatologia
Modelos Animais de Doenças
Seleção Artificial
Genótipo
Hiperglicemia/dietoterapia
Hiperglicemia/etiologia
Mitocôndrias/enzimologia
Óxido Nítrico/análise
Óxido Nítrico/metabolismo
Limites: Animais
Masculino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1132219
Autor: Rudnik, Loanda Aparecida Cabral; Lyra, Amanda Martinez; Barboza, Fernanda Malaquias; Klein, Traudi; Kanunfre, Carla Cristine; Farago, Paulo Vitor; Zanin, Sandra Maria Warumby; Nadal, Jessica Mendes.
Título: PEG-PCL Nanocapsules Containing Curcumin: Validation of HPLC Method for Analyzing Drug-loading Efficiency, Stability Testing and Cytotoxicity on NIH-3T3 Cell Line
Fonte: Braz. arch. biol. technol;63:e20200234, 2020. tab, graf.
Idioma: en.
Projeto: Conselho Nacional de Pesquisa e Desenvolvimento.
Resumo: Abstract Curcumin (CUR) shows potential use for treating cancer. However, CUR has low solubility and reduced bioavailability, which limit its clinical effect. Therefore, the development of nanocarriers can overcome these problems and can ensure the desired pharmacological effect. In addition, it is mandatory to prove the quality, the efficacy, and the safety for a novel nanomedicine to be approved. In that sense, this paper aimed (a) to prepare CUR-loaded polyethylene glycol-poly(ε-caprolactone) nanocapsules; (b) to validate an analytical method by high performance liquid chromatography (HPLC) for quantifying CUR in these nanoformulations; (c) to evaluate the physicochemical stability of these formulations; and to investigate their cytotoxicity on NIH-3T3 mouse fibroblast cells. The HPLC method was specific to CUR in the loaded nanocapsules, linear (r = 0.9994) in a range of 10.0 to 90.0 µg.mL-1 with limits of detection and quantification of 0.160 and 0.480 µg.mL-1, respectively. Precision was demonstrated by a relative standard deviation lower than 5%. Suitable accuracy (102.37 ± 0.92%) was obtained. Values of pH, particle size, polydispersity index, and zeta potential presented no statistical difference (p > 0.05) for CUR-loaded nanoparticles. No cytotoxicity was observed against NIH-3T3 mouse embryo fibroblast cell line using both the tetrazolium salt and sulforhodamine B assays. In conclusion, a simple and inexpensive HPLC method was validated for the CUR quantification in the suspensions of nanocapsules. The obtained polymeric nanocapsules containing CUR showed suitable results for all the performed assays and can be further investigated as a feasible novel approach for cancer treatment.
Descritores: Curcumina/farmacologia
Células-Tronco Embrionárias/efeitos dos fármacos
Fibroblastos/efeitos dos fármacos
-Cromatografia Líquida de Alta Pressão
Testes de Toxicidade
Nanotecnologia
Células NIH 3T3
Embrião de Mamíferos/citologia
Nanocápsulas
Limites: Animais
Camundongos
Responsável: BR1.1 - BIREME


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Id: biblio-1124214
Autor: Erfanizadeh, Mahboobeh; Noorafshan, Ali; Reza-Namavar, Mohammad; Karbalay-Doust, Saied; Talaei-Khozani, Tahereh.
Título: Curcumin prevents neuronal loss and structural changes in the superior cervical (sympathetic) ganglion induced by chronic sleep deprivation, in the rat model
Fonte: Biol. Res;53:31, 2020. graf.
Idioma: en.
Projeto: Shiraz University of Medical Sciences.
Resumo: BACKGROUND: In modern societies, sleep deprivation is a serious health problem. This problem could be induced by a variety of reasons, including lifestyle habits or neurological disorders. Chronic sleep deprivation (CSD) could have complex biological consequences, such as changes in neural autonomic control, increased oxidative stress, and inflammatory responses. The superior cervical ganglion (SCG) is an important sympathetic component of the autonomic nervous system. CSD can lead to a wide range of neurological consequences in SCG, which mainly supply innervations to circadian system and other structures. As the active component of Curcuma longa, curcumin possesses many therapeutic properties; including neuroprotective. This study aimed to evaluate the effect of CSD on the SCG histomorphometrical changes and the protective effect of curcumin in preventing these changes. METHODS: Thirty-six male rats were randomly assigned to the control, curcumin, CSD, CSD + curcumin, grid floor control, and grid floor + curcumin groups. The CSD was induced by a modified multiple platform apparatus for 21 days and animals were sacrificed at the end of CSD or treatment, and their SCGs removed for stereological and TUNEL evaluations and also spatial arrangement of neurons in this structure. RESULTS: Concerning stereological findings, CSD significantly reduced the volume of SCG and its total number of neurons and satellite glial cells in comparison with the control animals ( P < 0.05). Treatment of CSD with curcumin prevented these decreases. Furthermore, TUNEL evaluation showed significant apoptosis in the SCG cells in the CSD group, and treatment with curcumin significantly decreased this apoptosis ( P < 0.01). This decrease in apoptosis was observed in all control groups that received curcumin. CSD also changed the spatial arrangement of ganglionic neurons into a random pattern, whereas treatment with curcumin preserved its regular pattern. CONCLUSIONS: CSD could potentially induce neuronal loss and structural changes including random spatial distribution in the SCG neurons. Deleterious effects of sleep deprivation could be prevented by the oral administration of curcumin. Furthermore, the consumption of curcumin in a healthy person might lead to a reduction of cell death.
Descritores: Privação do Sono/patologia
Privação do Sono/tratamento farmacológico
Anti-Inflamatórios não Esteroides/farmacologia
Gânglio Cervical Superior/efeitos dos fármacos
Curcumina/farmacologia
-Ratos Sprague-Dawley
Limites: Animais
Masculino
Ratos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-888308
Autor: Bondan, Eduardo; Cardoso, Carolina; Martins, Maria de Fátima.
Título: Curcumin decreases astrocytic reaction after gliotoxic injury in the rat brainstem / Curcumina reduz a reação astrocitária após injúria gliotóxica no tronco encefálico de ratos
Fonte: Arq. neuropsiquiatr;75(8):546-552, Aug. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Recent studies have demonstrated that curcumin (Cur) has antioxidant, anti-inflammatory and anti-fibrotic effects. Ethidium bromide (EB) injections into the central nervous system (CNS) are known to induce local oligodendroglial and astrocytic loss, resulting in primary demyelination and neuroinflammation. Peripheral astrogliosis is seen around the injury site with increased immunoreactivity to glial fibrillary acidic protein (GFAP). This investigation aimed to evaluate the effect of Cur administration on astrocytic response following gliotoxic injury. Wistar rats were injected with EB into the cisterna pontis and treated, or not, with Cur (100 mg/kg/day, intraperitoneal route) during the experimental period. Brainstem sections were collected at 15, 21 and 31 days after EB injection and processed for GFAP immunohistochemical staining. Astrocytic reactivity was measured in a computerized system for image analysis. In Cur-treated rats, the GFAP-stained area around the lesion was significantly smaller in all periods after EB injection compared to untreated animals, showing that Cur reduces glial scar development following injury.

RESUMO Estudos recentes têm demonstrado que a curcumina (Cur) possui efeitos antioxidantes, anti-inflamatórios e antifibróticos. Sabe-se que a injeção de brometo de etídio (EB) no sistema nervoso central induz a perda oligodendroglial e astrocitária, resultando em desmielinização primária e neuroinflamação. Astrogliose periférica é observada ao redor da lesão com aumento da imunorreatividade à proteína glial fibrilar ácida (GFAP). A presente investigação objetivou avaliar o efeito da Cur sobre a resposta astrocitária após injúria gliotóxica. Ratos Wistar foram injetados com EB na cisterna basal e tratados ou não com Cur (100 mg/kg/dia, via intraperitoneal) durante o período experimental. Amostras do tronco encefálico foram coletadas aos 15, 21 e 31 dias pós-injeção de EB e processadas para estudo imuno-histoquímico para a GFAP. A reatividade astrocitária foi medida em um sistema computadorizado para análise de imagem. Nos ratos tratados com Cur, a área marcada para GFAP foi significantemente menor em todos os períodos pós-injeção de EB, indicando que a Cur reduz o desenvolvimento da cicatriz glial após injúria.
Descritores: Tronco Encefálico/patologia
Astrócitos/efeitos dos fármacos
Doenças Desmielinizantes/patologia
Curcumina/uso terapêutico
-Coloração e Rotulagem
Tronco Encefálico/efeitos dos fármacos
Anti-Inflamatórios não Esteroides/uso terapêutico
Anti-Inflamatórios não Esteroides/farmacologia
Astrócitos/patologia
Doenças Desmielinizantes/induzido quimicamente
Ratos Wistar
Curcumina/farmacologia
Modelos Animais de Doenças
Etídio
Proteína Glial Fibrilar Ácida/metabolismo
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-1153294
Autor: Demircioglu, Busra; Aydin, Yasemin; Orta-Yilmaz, Banu; Yildizbayrak, Nebahat; Erkan, Melike.
Título: Effects of Curcumin on Sodium Arsenite Induced Neoplastic Cell Transformation in Balb/c 3T3 Cells
Fonte: Braz. arch. biol. technol;64:e21200093, 2021. tab, graf.
Idioma: en.
Projeto: Istanbul University Scientific Research Projects.
Resumo: HIGHLIGHTS Sodium arsenite can cause neoplastic transformation in cells. Curcumin reduced cell viability and increased LDH activity in transformed Balb/c 3T3 cells. Curcumin caused DNA damage in transformed Balb/c 3T3 cells. Curcumin may play a protective role in sodium arsenite-induced toxicity.

Abstract Arsenic is a toxic substance that spreads widely around the environment and accumulates as metalloid in the earth's crust. Arsenic and its derivatives are found in drinking water, nutrients, soil, and air. Exposure to arsenic is associated with lung, blood, skin cancer and various lesions. Curcumin is a polyphenolic compound derived from Curcuma longa (turmeric) rhizome and is one of the main curcuminoids. Curcumin is known to be antioxidant, antibacterial, anti-inflammatory, analgesic effects. This study aimed to investigate the potential of sodium arsenite to transform embryonic fibroblast cells and to evaluate the cytotoxic and genotoxic effects of curcumin in neoplastic transformed cells. Neoplastic cells transformation was induced by sodium arsenite in Balb/c 3T3 cells at the end of 32 days. After transformation assay, the transformed cells were treated with various concentration of curcumin to evaluate cell viability, lactate dehydrogenase activity and DNA damage for 24h. The results revealed that curcumin decreased cell viability and increased the activity of lactate dehydrogenase enzyme in neoplastic transformed Balb/c 3T3 cells. In conclusion, the results demonstrated that curcumin has an anticancer effect on neoplastic transformed Balb/c 3T3 cells by causing DNA damage.
Descritores: Arsênio/toxicidade
Dano ao DNA
Transformação Celular Neoplásica
Curcumina/farmacologia
Fibroblastos/efeitos dos fármacos
-Células 3T3 BALB
Fibroblastos/patologia
Limites: Animais
Camundongos
Responsável: BR1.1 - BIREME


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Id: biblio-896419
Autor: Behroozi-Lak, Tahereh; Ebrahimpour, Malahat; Zarei, Leila; Pourjabali, Masoumeh; Farhad, Negin; Mohaddesi, Hamideh.
Título: Systemic administration of curcumin nanoparticles protects ischemia-reperfusion injury in ovaries: An animal model study
Fonte: Rev. Assoc. Med. Bras. (1992);64(1):22-31, Jan. 2018. tab, graf.
Idioma: en.
Resumo: Summary Objective: Ovarian torsion must be diagnosed and treated as early as possible. The aim of the present study was to investigate the effects of intraperitoneal administration of nanocurcumin on ischemia-reperfusion injury in ovaries. Method: Thirty-five (35) healthy female Wistar rats weighing approximately 250 g were randomized into seven experimental groups (n=5): Group SSG - The rats underwent only laparotomy. Group I: A 3-hour ischemia only. Group I/R: A 3-hour ischemia and 3-hour reperfusion. Group I/C: A 3-hour ischemia only, and 1 mg/kg intraperitoneal administration of curcumin 2.5 hours after induction of ischemia. Group I/R/C: A 3-hour ischemia, 3-hour reperfusion, and 1 mg/kg intraperitoneal administration of curcumin 2.5 hours after induction of ischemia. Group I/NC: A 3-hour ischemia only and 1 mg/kg intraperitoneal administration of nanocurcumin 2.5 hours after induction of ischemia. Group I/R/C: A 3-hour ischemia, 3-hour reperfusion and 1 mg/kg intraperitoneal administration of nanocurcumin 2.5 hours after induction of ischemia. Results: Nanocurcumin-treated animals showed significantly improved development of ischemia and reperfusion tissue injury compared to those in the other groups (p<0.05). Significant higher values of SOD, tGSH, GPO, GSHRd and GST were observed in I/R/NC animals compared to those in the other groups (p<0.05). The damage indicators (NOS, MDA, MPO and DNA damage level) were significantly lower in I/R/NC animal compared to those of other groups (p<0.05). Conclusion: Intraperitoneal administration of nanocurcumin can be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia.
Descritores: Ovário/irrigação sanguínea
Traumatismo por Reperfusão/tratamento farmacológico
Curcumina/administração & dosagem
Nanopartículas/administração & dosagem
Isquemia/tratamento farmacológico
Antioxidantes/administração & dosagem
-Administração Cutânea
Traumatismo por Reperfusão/patologia
Ratos Wistar
Modelos Animais de Doenças
Injeções Intraperitoneais
Isquemia/patologia
Limites: Humanos
Animais
Feminino
Ratos
Responsável: BR1.1 - BIREME


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Consolaro, Alberto
Silva, Léa Assed Bezerra da
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Id: biblio-1178040
Autor: Silva, Raquel Assed Bezerra da; Oliveira, Daniela Silva Barroso de; Oliveira, Katharina Morant Holanda de; Pucinelli, Carolina Maschietto; Nelson Filho, Paulo; Consolaro, Alberto; Novaes Jr, Arthur Belém; Silva, Lea Assed Bezerra da.
Título: Photosensitizers and exposure times to light showed tissue compatibility in isogenic mice / Fotossensibilizadores e tempo de exposição a luz em relação a compatibilidade de tecidos em ratos isogênicos
Fonte: Braz. dent. sci;24(2):1-12, 2021. tab, ilus.
Idioma: en.
Resumo: Objective: The aim of this study was to evaluate the subcutaneous tissue response after different protocols to photodynamic therapy (PDT). In Phase 1, were tested the diode laser (used for 1min) associated to the photosensitizer phenothiazine chloride solution (PCS) in different concentrations. In Phase 2 ­ the diode laser and LED were tested associated to two different photosensitizers, PCS and Curcumin, in different exposure times of light application. Material and Methods: After 7, 21 and 63-days the animals were euthanized and the subcutaneous tissue processed to histological analysis. Qualitative and semi-quantitative descriptions of the inflammatory process and immunohistochemical technique were performed. The obtained data were analyzed by Kruskal-Wallis and Dunn's post-test (α= 0.5). Results: On Phase 1, the tissue response was very similar among the groups. For the inflammatory infiltrate, PCS with concentration of 10mg/mL exhibited the most intense reaction (p > 0.05). On Phase 2, at 7-days period, the analyzed parameters presented small magnitude and after 21 and 63-days, all the parameters demonstrated tissue compatibility. Conclusion: Both photosensitizers presented proper tissue compatibility regardless the different concentrations used on Phase 1 and different durations of light exposure on Phase 2 (AU)

Objetivo: Este estudo avaliou a resposta do tecido subcutâneo após terapia fotodinâmica, utilizando na Fase 1 - laser diodo por 1min e solução fotossensibilizadora de cloreto de fenotiazina (CF) em diferentes concentrações e Fase 2 - laser diodo e LED e dois fotossensibilizadores, CF e Curcumina, em diferentes tempos de exposição da aplicação de luz. Material e Métodos: Após 7, 21 e 63 dias, foram realizadas descrições qualitativas e semiquantitativas do processo inflamatório e técnica de imunoistoquímica. Os dados foram analisados pelo pós-teste de Kruskal-Wallis e Dunn (α = 0,5). Resultados: Na Fase 1, a resposta do tecido foi muito semelhante. O infiltrado inflamatório, na concentração de 10 mg / mL, exibiu reação mais intensa (p > 0,05). Na Fase 2, aos 7 dias, os parâmetros analisados apresentaram pequena magnitude. Aos 21 e 63 dias, todos os parâmetros demonstraram compatibilidade com o tecido. Conclusão: Ambos os fotossensibilizadores apresentaram compatibilidade de tecido adequada, independentemente das diferentes concentrações utilizadas na Fase 1 e diferentes durações de exposição à luz na Fase 2 (AU)
Descritores: Fotoquimioterapia
Ratos Endogâmicos
Curcumina
Tela Subcutânea
Limites: Animais
Camundongos
Tipo de Publ: Estudo Comparativo
Responsável: BR243.1 - Serviço Técnico de Biblioteca e Documentação


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Id: biblio-894005
Autor: Martinez, Carlos Augusto Real; Oliveira, Vitor Piquera de; Campos, Fabio Guilherme; Kanno, Danilo Toshio; Fernandez, Oscar Orlando Araya; Kadri, Caled Jaoudat; Pereira, José Aires.
Título: Influence of the application of enemas with oily extract of curcumin (Curcuma longa) on the tissue content of neutral and acidic mucins in the colonic mucosa without fecal stream / Influência da aplicação de clisteres contendo extrato oleoso de curcumina (Curcuma longa) no conteúdo tecidual de mucinas neutras e ácidas na mucosa cólica sem trânsito intestinal
Fonte: J. coloproctol. (Rio J., Impr.);37(4):312-319, Oct.-Dec. 2017. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: ABSTRACT Purpose: To verify if the application of enemas containing oily extracts of curcumin preserves the tissue content of mucins in the glands of the colonic mucosa without fecal stream. Method: Thirty-six Wistar rats were submitted to diversion of the fecal stream by proximal colostomy and distal mucous fistula. The animals were subdivided into three groups, and accordingly received enemas with saline and oily extract of curcumin at concentrations of 50 mg/kg/day or 200 mg/kg/day. After two or four weeks of intervention, the irrigated colic segments were removed. Neutral and acidic mucins were identified by Periodic-acid Schiff and Alcian-Blue techniques, respectively. The content of both mucin subtypes was measured by computerized morphometry. Mann-Whitney test was used to analyze the results, adopting a significance level of 5% (p ≤ 0.05). Results: There was an increase in the tissue content of neutral mucins in animals treated with curcumin at a concentration of 50 mg/kg/day for four weeks, whereas in the group treated with 200 mg/kg/day there was an increase independent of the time of intervention. The content of acidic mucins increased in animals treated with 200 mg/kg/day regardless of the intervention time, whereas in those treated with 50 mg/kg/day an increase was observed only after four weeks. Conclusion: Enemas with curcumin preserve the content of neutral and acidic mucins in the colonic epithelium without fecal stream.

RESUMO Objetivo: Verificar se a aplicação de clisteres com extrato oleoso de curcumina preserva o conteúdo de mucinas nas glândulas da mucosa cólica sem trânsito intestinal. Método: Trinta e seis ratos Wistar foram submetidos à derivação intestinal por colostomia proximal e fístula mucosa distal. Os animais foram subdivididos em três grupos, segundo receberem clisteres com soro fisiológico 0,9%, extrato oleoso de curcumina nas concentrações de 50 mg/kg/dia ou 200 mg/kg/dia. Após duas ou quatro semanas de intervenção foram removidos os segmentos cólicos irrigados. As mucinas neutras e ácidas foram identificadas pelas técnicas do PAS e Alcian-Blue, respectivamente. O conteúdo tecidual de ambos os subtipos de mucinas foi mensurado por morfometria computadorizada. Utilizou-se teste de Mann-Whitney para análise dos resultados adotando-se nível de significância de 5% (p ≤ 0,05). Resultados: Houve aumento no conteúdo de mucinas neutras nos animais tratados com curcumina na concentração de 50 mg/kg/dia por quatro semanas, enquanto nos tratados com 200 mg/kg/dia houve aumento independente do tempo de intervenção. O conteúdo de mucinas ácidas aumentou nos animais tratados com 200 mg/kg/dia independente do tempo de intervenção, enquanto nos tratados com 50 mg/kg/dia encontrou-se aumento apenas após quatro semanas. Conclusão: Clisteres com curcumina preservam o conteúdo de mucinas neutras e ácidas no epitélio cólico sem trânsito intestinal.
Descritores: Curcumina/farmacologia
Mucinas/efeitos dos fármacos
-Ratos Wistar
Colite/tratamento farmacológico
Limites: Animais
Ratos
Responsável: BR545.3 - Biblioteca ICBS



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