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Id: biblio-886716
Autor: BASTOS, ISLA V G A; OLIVEIRA, TATIANE B DE; RODRIGUES, MARIA D; MILITÃO, GARDÊNIA C G; SILVA, TERESINHA G DA; TURATTI, IZABEL C C; LOPES, NORBERTO P; MELO, SEBASTIÃO J DE.
Título: Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)
Fonte: An. acad. bras. ciênc;89(2):991-1001, Apr.-June 2017. tab.
Idioma: en.
Resumo: ABSTRACT Phoradendron mucronatum and P. microphyllum are plants that found in tropical and subtropical areas, used in traditional medicine and popularly known as mistle-thrush. The aim of this study was to identify the chemical constituents of different leaf extracts from P. mucronatum and P. microphyllum and assess cytotoxic activity against strains from a human tumour cells. Extracts obtained with hexane, dichloromethane, chloroform and ethyl acetate from the leaves were analysed by gas chromatography coupled with mass spectrometry (GC-MS) and the cytotoxicity was assessed by the MTT method (bromide (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)). The tested human tumour cells were NCI-H292 (human pulmonar mucoepidermoid carcinoma), MCF-7 (human breast adenocarcinoma) and HEp-2 (epidermoid carcinoma of the larynx). Analysis by GC/MS of the extracts from leaves of P. microphyllum and P. mucronatum detected 51 different compounds, such as alkaloids, diterpenes, triterpenes, sterols, alcohols, aldehydes, fatty acids and hydrocarbons. In the cytotoxic evaluation, hexane and ethyl acetate extracts from the leaves P. microphyllum inhibited cell growth of NCI-H292 strains (72.97%) and HEp-2 (87.53%), respectively. The extracts of P. mucronatum species showed an inhibitory effect towards NCI-H292 (83.19%/hexane), MCF-7 (88.69%/dichloromethane) and HEp-2 (93.40%/hexane). The extracts showed cytotoxic activity against the tested strains, especially the P. mucronatum, which presented the highest percentages of inhibition of cell growth.
Descritores: Extratos Vegetais/química
Folhas de Planta/química
Viscaceae/química
Phoradendron/química
-Sais de Tetrazólio
Tiazóis
Extratos Vegetais/farmacologia
Clorofórmio/química
Reprodutibilidade dos Testes
Testes de Toxicidade
Linhagem Celular Tumoral
Proliferação de Células/efeitos dos fármacos
Células MCF-7
Hexanos/química
Cromatografia Gasosa-Espectrometria de Massas
Cloreto de Metileno/química
Acetatos/química
Limites: Humanos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: lil-781389
Autor: Habermann, E; Pontes, F C; Pereira, V C; Imatomi, M; Gualtieri, S C J.
Título: Phytotoxic potential of young leaves from Blepharocalyx salicifolius (Kunth) O. Berg (Myrtaceae) / Potencial fitotóxico de folhas jovens de Blepharocalyx salicifolius (Kunth) O. Berg
Fonte: Braz. j. biol;76(2):531-538, Apr.-June 2016. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Abstract The loss of leaves by plant species found in the Cerrado (Brazilian savanna) is an energetically expensive process due to adverse environmental conditions and predation by herbivory. The mature leaves have adaptations which minimize these events. However, the young individuals lack these structures and produce high leaf concentrations of secondary metabolites as a form of protection. These compounds can be used in bioprospection of natural herbicides. Thus, this study aimed to evaluate the phytotoxicity of hexane, ethyl acetate and aqueous extracts of young leaves from Blepharocalyx salicifolius (Kunth) O. Berg on the elongation of wheat coleoptiles (Triticum aestivum L.) and evaluate the potential phytotoxic of ethyl acetate extract on germination, growth and cell size of metaxylem of sesame (Sesamum indicum L.) seedlings. The hexane and ethyl acetate extracts inhibited the elongation of wheat coleoptiles at all concentrations; however, the most promising results were observed in coleoptile fragments treated with the ethyl acetate extract. This treatment changed the mean germination time and the synchrony of sesame seeds, inhibited the growth of shoots and roots, reduced the dry weight of seedlings, led to abnormalities in the seedlings and reduced the length of the metaxylem cells in the sesame seedlings. These results demonstrated the phytotoxic potential of young leaf extracts of B. salicifolius and the high phytotoxicity of the ethyl acetate extract in the initial development of S. indicum.

Resumo A perda das folhas pelas plantas do Cerrado é um processo energeticamente custoso devido às condições ambientais adversas e intensa predação por herbivoria. As folhas maduras possuem adaptações que minimizam esses eventos. Entretanto, os indivíduos jovens carecem dessas estruturas e produzem elevadas concentrações foliares de metabólitos secundários como forma de proteção. Esses compostos podem ser utilizados na bioprospecção de herbicidas naturais. Dessa forma, objetivamos com este estudo avaliar a fitotoxicidade dos extratos hexânico, acetato etílico e aquoso de folhas jovens de Blepharocalyx salicifolius (Kunth) O. Berg sobre o alongamento de coleóptilos estiolados de Triticum aestivum L. (trigo) e avaliar o potencial fitotóxico do extrato acetato etílico sobre a germinação, crescimento e comprimento das células do metaxilema de Sesamum indicum (gergelim). Os extratos hexânico e acetato etílico inibiram o alongamento dos coleóptilos em todas as concentrações testadas, entretanto os resultados mais promissores foram observados nos fragmentos submetidos ao extrato acetato etílico. Este extrato alterou o tempo médio de germinação e a sincronia das sementes de gergelim, inibiu o crescimento da parte aérea e subterrânea com redução da massa seca das plântulas, levou ao surgimento acentuado de anomalias e causou redução no comprimento médio das células do metaxilema de plântulas de gergelim. Assim, ficou comprovado o potencial fitotóxico dos extratos de folhas jovens de B. salicifolius e a acentuada fitotoxicidade do extrato acetato etílico sobre o desenvolvimento inicial de S. indicum.
Descritores: Triticum/efeitos dos fármacos
Triticum/fisiologia
Folhas de Planta/efeitos dos fármacos
Germinação/efeitos dos fármacos
Myrtaceae
Hexanos/farmacologia
Acetatos/farmacologia
-Brasil
Extratos Vegetais/farmacologia
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/efeitos dos fármacos
Folhas de Planta/crescimento & desenvolvimento
Plântula/efeitos dos fármacos
Herbicidas/farmacologia
Responsável: BR1.1 - BIREME


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Id: lil-515243
Autor: Valdivia Infantas, Melinda Martina; Quevedo, María Graciela.
Título: Neuropatía por n-hexano / N-hexane neuropathy
Fonte: Rev. Soc. Peru. Med. Interna;21(4):174-177, oct.-dic. 2008. ilus.
Idioma: es.
Descritores: Hexanos
Hidrocarbonetos
Tipo de Publ: Revisão
Responsável: PE1.1 - Oficina Universitária de Biblioteca


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Id: lil-732391
Autor: Uyar, Belkiz; Sivrikoz, Oya Nermin; Ozdemir, Ugur; Dasbasi, Teslima; Sacar, Handan.
Título: Histological investigation of the effect of soybean (Glycine max) extracts on the collagen layer and estrogen receptors in the skin of female rats
Fonte: Clinics;69(12):854-861, 2014. tab, graf.
Idioma: en.
Resumo: OBJECTIVES: The purpose of this study was to analyze the effects of soybean extracts obtained using different extraction methods on the skin of female rats. METHOD: A total of 64 female Sprague-Dawley rats were divided into 8 equal groups. Various extracts were administered to the female rats by oral gavage for one month. The groups comprised carboxymethyl cellulose-free control, carboxymethyl cellulose-plus control, 100-mg/kg n-hexane extract, 200-mg/kg n-hexane extract, 100-mg/kg ethyl acetate extract, 200-mg/kg ethyl acetate extract, 100-mg/kg ethanol extract and 200-mg/kg ethanol extract groups. The thickness of the collagen layer and the number of estrogen receptor-positive cells were evaluated. RESULTS: All the extract-treated groups showed a statistically significant decrease in the number of estrogen receptor-positive cells compared with the control groups. Regarding the thickness of the collagen layer, only the 200-mg/kg ethyl acetate extract-treated group showed a significant increase compared with the control groups (p<0.05). CONCLUSIONS: Our data suggest that oral intake of three different total soybean extracts might have positive estrogenic effects on the skin and that only a high-dose ethyl acetate extract can increase the expression of collagen, which may prove to be beneficial for postmenopausal facial skin. ...
Descritores: Colágeno/análise
Extratos Vegetais/farmacologia
Receptores Estrogênicos/análise
Pele/efeitos dos fármacos
Soja/química
-Acetatos/farmacologia
Cromatografia Líquida de Alta Pressão
Colágeno/efeitos dos fármacos
Etanol/farmacologia
Hexanos/farmacologia
Imuno-Histoquímica
Fitoestrógenos/farmacologia
Ratos Sprague-Dawley
Valores de Referência
Reprodutibilidade dos Testes
Receptores Estrogênicos/efeitos dos fármacos
Pele/química
Fatores de Tempo
Limites: Animais
Feminino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-723584
Autor: Silva, Pâmela Souza; Suzuki, Érika Yoko; Moreira, Ana Paula; Raposo, Nádia Rezende Barbosa; Alves, Tânia Maria Almeida; Viccini, Lyderson Facio.
Título: Stachytarpheta gesnerioides Cham: chemical composition of hexane fraction and essential oil, antioxidant and antimicrobial activities / Stachytarpheta gesnerioides Cham: composición química de la fracción hexánica y aceite esencial, actividades antioxidante y antimicrobiana
Fonte: Bol. latinoam. Caribe plantas med. aromát;11(6):542-548, nov. 2012. tab.
Idioma: en.
Resumo: In the present study we investigated the chemical composition of hexane fraction and essential oil of Stachytarpheta gesnerioides (Verbenaceae) by GC-MS, total phenol and flavonoid contents. The antioxidant capacity and antimicrobial activity were investigated in five extracts of leaves of S. gesnerioides. Aqueous and 100 percent ethanol extracts were prepared by dynamic maceration. Hexane, ethyl acetate and methanol extracts were prepared by Soxhlet extraction. The essential oil (EO) and hexane fraction (HF) are mainly composed by guaiol. Moreover, the HF is also rich in the monoterpene alpha-pinene. The total phenol content ranged from 0.85 to 22.74 mg gallic acid equivalent /100mg dry extract at Folin–Ciocalteu's reagent method. The total flavonoid concentration ranged from 0.68 to 13.65 mg rutin equivalent /100mg dry extract, detected using 8 percent aluminium chloride. The ethyl acetate extract (IC50=9.41 ug/ml) showed the highest antioxidant activity. The extracts were found to be effective to inhibit the microorganisms tested.

Se han investigado la composición química de la fracción hexánica (FH) y aceite esencial (AE) de Stachytarpheta gesnerioides (Verbenaceae) por GC-MS, el contenido de fenoles totales y flavonoides. La capacidad antioxidante y actividad antimicrobiana fueron investigadas en cinco extractos de hojas de S gesnerioides. Extractos acuosos y etanolico fueron preparados por la maceración dinámica y extracción continua en Soxhlet con hexano, acetato de etilo y metanol. Las fracciones AE y FH están compuestas principalmente por guaiol. La fracción FH es también rica en alfa-pineno. El contenido de fenoles totales varió desde 0,85 hasta 22,74 mg de ácido gálico/100 mg de extracto seco (Folin-Ciocalteu). La concentración total de flavonoides varió desde 0,68 hasta 13,65 mg en equivalentes de rutina/100 mg de extracto seco, que se detectó mediante reacción con cloruro de aluminio al 8 por ciento. El extracto de acetato de etilo (CI50=9,41 ug/ml) enseño la más grande actividad antioxidante. Los extractos se encontraron eficaces para inhibir los microorganismos ensayados.
Descritores: Óleos Voláteis/química
Antibacterianos/farmacologia
Antioxidantes/farmacologia
Verbenaceae/química
-Fenóis/análise
Flavonoides/análise
Cromatografia Gasosa-Espectrometria de Massas
Hexanos/química
Testes de Sensibilidade Microbiana
Folhas de Planta
Responsável: CL1.1 - Biblioteca Central


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Id: lil-687010
Autor: Buitrago Díaz, Alexis; Rojas Vera, Janne; Rojas Fermín, Luís; Morales Méndez, Antonio; Aparicio Zambrano, Rosa; Rodríguez Contreras, Luís.
Título: Composition of the essential oil of leaves and roots of Allium schoenoprasum L. (Alliaceae) / Composición del aceite esencial de hojas y raíces de Allium schoenoprasum L
Fonte: Bol. latinoam. Caribe plantas med. aromát;10(3):218-221, mayo 2011. ilus, tab.
Idioma: en.
Resumo: Essential oil from leaves and roots of Allium schoenoprasum L. (Alliaceae) were analyzed by GC/MS. The oils extracted by hydrodistillation yielded 0.02 percent and 0.03 percent for leaves and roots, respectively. Five (99.12 percent leaves) and four (98.32 percent roots) components were identified being bis-(2-sulfhydryethyl)-disulfide (72.06 percent leaves, 56.47 percent roots) the major constituent on the two oil samples while 2,4,5-trithiahexane (5.45 percent leaves, 15.90 percent roots) and tris (methylthio)-methane (4.01 percent leaves, 12.81 percent roots) were detected in lower amounts.

Los aceites esenciales de hojas y raíces de Allium schoenoprasum L. (Alliaceae fueron analizados por CG/EM. La extracción por hidrodestilación mostró un rendimiento de 0.02 por ciento y 0.03 por ciento para las hojas y raíces, respectivamente. Cinco (99.12 por ciento hojas) y cuatro (98.32 por ciento raíces) compuestos fueron identificados siendo el bis-(2-sulfidietil)-disulfuro (72.06 por ciento hojas, 56.47 por ciento raíces) el compuesto mayoritario en los dos aceites mientras que 2,4,5-tritiahexano (5.45 por ciento hojas, 15.90 por ciento raíces) y tris (metilthio)-metano (4.01 por ciento hojas, 12.81 por ciento raíces) fueron observados en menores cantidades.
Descritores: Óleos Voláteis/química
Cebolinha-Francesa/química
Folhas de Planta/química
Sulfetos/análise
-Cromatografia Gasosa-Espectrometria de Massas
Hexanos/análise
Metano/análise
Raízes de Plantas/química
Responsável: CL1.1 - Biblioteca Central


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Id: lil-663708
Autor: Patiño, Arley Camilo; López, Jéssica; Aristizábal, Mónica; Quintana, Juan Carlos.
Título: Efecto inhibitorio de extractos de Renealmia alpinia Rottb. Maas (Zingiberaceae) sobre el veneno de Bothrops asper (mapaná) / Evaluation of the inhibitory effect of extracts from leaves of Renealmia alpinia Rottb. Maas (Zingiberaceae) on the venom of Bothrops asper (mapaná)
Fonte: Biomédica (Bogotá);32(3):365-374, jul.-set. 2012. ilus, graf, tab.
Idioma: es.
Resumo: Introducción. La medicina tradicional es una invaluable fuente de investigación de nuevos remedios como complemento para el tratamiento del accidente ofídico, considerado como un grave problema de salud pública a nivel mundial. Objetivo. Este trabajo de investigación pretende comprobar la capacidad de neutralizar los efectos hemorrágicos, coagulantes y proteolíticos, de los extractos de hojas de Renealmia alpinia, usada tradicionalmente por los indígenas del Chocó (Colombia) contra la mordedura de la serpiente Bothrops asper, causante de la gran mayoría de los accidentes ofídicos en nuestro país. Materiales y métodos. Se llevaron a cabo ensayos de toxicidad aguda y de actividad analgésica in vivo de R. alpinia. Además, se hicieron ensayos in vitro sobre inhibición de las actividades coagulante, hemolítica y proteolítica del veneno de B. asper. Resultados. El presente estudio demuestra que R. alpinia no produce efectos tóxicos en animales de experimentación; además, presenta efectos analgésicos in vivo y antiofídicos in vitro,y protege contra los efectos letales del veneno de B. asper, in vivo. Conclusión. Renealmia alpinia puede ser una buena alternativa terapéutica como complemento al tratamiento con antiveneno en el accidente ofídico, por sus efectos analgésicos y antiofídicos.

Introduction. Traditional medicine is an invaluable source of research into new medicines as a supplement for the treatment of snakebite, considered as a serious public health problem worldwide. The extracts of the medicinal plant, Renealmia alpina, have been used traditionally by indigenous people of Chocó (Colombia) against Bothrops asper snakebite, a snake responsible for the majority of snakebite accidents in Colombia. Objective. The ability of extracts of R. alpinia leaves was tested for its ability to neutralize the hemorrhagic, coagulant and proteolytic effects of the snakebite venom of B. asper. Materials and methods. The acute toxicity tests and analgesic activity of R. alpina were evaluated in vivo. In addition, tests were undertaken in in vitro conditions to demonstrate inhibition of coagulant, haemolytic and proteolytic activity of the B. asper venom. Results. Renealmia alpinia extracts had no toxic effects in experimental animals and also provided analgesic and antiophidian effects and protection against the lethal effects of the venom of B. asper. Conclusion. Renealmia. alpinia was an effective therapeutic alternative in association with antivenom treatment in the event of a B. asper snakebite accident. It was demonstrated to protect against the lethal effects and provided analgesic properties as well.
Descritores: Bothrops
Venenos de Crotalídeos/antagonistas & inibidores
Fitoterapia
Extratos Vegetais/uso terapêutico
Folhas de Planta/química
Mordeduras de Serpentes/tratamento farmacológico
Zingiberaceae
-Acetatos
Analgésicos/uso terapêutico
Analgésicos/toxicidade
Coagulação Sanguínea/efeitos dos fármacos
Venenos de Crotalídeos/toxicidade
Avaliação Pré-Clínica de Medicamentos
Etanol
Hexanos
Hemólise/efeitos dos fármacos
Metanol
Cloreto de Metileno
Camundongos Endogâmicos BALB C
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/farmacologia
Extratos Vegetais/toxicidade
Proteólise/efeitos dos fármacos
Solventes
Limites: Animais
Feminino
Masculino
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: CO332 - Facultad de Medicina


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Id: lil-648053
Autor: Miranda-Cruz, Edith; Espinosa-Moreno, Judith; Centurión-Hidalgo, Dora; Velázquez-Martínez, José Rodolfo; Alor-Chávez, Maricela de Jesús.
Título: Actividad antimicrobiana de extractos de Psidium friedrichsthalianum L., Pterocarpus hayesii L., Tynanthus guatemalensis L. y Spondias purpurea L / Antimicrobial activity of Psidium friedrichsthalianum L., Pterocarpus hayesii L., Tynanthus guatemalensis L. and Spondias purpurea L. extracts
Fonte: Bol. latinoam. Caribe plantas med. aromát;11(4):354-361, jul. 2012. tab.
Idioma: es.
Projeto: Universidad Juárez Autónoma de Tabasco. Programa para el Fortalecimiento de la Investigación de Cuerpos Académicos.
Resumo: There is not much information on antimicrobial activity presented by several species traditionally used as medicinal plants in Tabasco. Antimicrobial activity of ethanolic and hexanic extracts from leaf and bark of guayaba agria (Psidium friedrichsthalianum L.), palo de sangre (Pterocarpus hayesii L.) chichimecate (Tynanthus guatemalensis L.) and ciruela (Spondias purpurea L.) was evaluated for against Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 14028 and Bacillus cereus ATCC 11778 by the use of agar diffusion method. Results indicate that the hexanic extract of every one of the plant species presented antimicrobial activity on at least one of the evaluated microorganisms meanwhile bark hexanic extracts did not present activity against the three microorganisms tested. The extracts that presented a Minimal Inhibitory Concentration (MIC) of < 7.50 mg mL-1 against B. cereus were both leaf ethanolic of P. friedrichsthalianum and S. purpurea and the T. guatemalensis leaf hexanic extract as well as P. friedrichsthalianum bark hexánico extract against S. aureus and S. typhimurium.

Existe poca información sobre la actividad antimicrobiana que pueden presentar varias plantas que han sido reportadas con uso medicinal tradicional en el Estado de Tabasco. Se evaluó la actividad antimicrobiana de los extractos etanólico y hexánico de hoja y corteza de cuatro plantas utilizadas como medicinales: guayaba agria (Psidium friedrichsthalianum L.), palo de sangre (Pterocarpus hayesii L.), chichimecate (Tynanthus guatemalensis L.) y ciruela (Spondias purpurea L.). La actividad antimicrobiana se evaluó contra Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 14028 y Bacillus cereus ATCC 11778 mediante el método de difusión en agar. Los resultados indican que el extracto hexánico de cada una de las plantas presentaron actividad antimicrobiana al menos en uno de los microorganismos evaluados mientras que los extractos hexánicos de corteza no presentaron actividad contra ninguno de los tres microorganismos ensayados. Los extractos que presentaron una Concentración Mínima Inhibitoria (CMI) igual o menor de 7.50 mg mL-1 contra B. cereus fueron los etanólicos de hoja de P. friedrichsthalianum y S. purpurea y el hexánico de hoja de T. guatemalensis así como el extracto hexánico de corteza de P. friedrichsthalianum contra S. aureus y S. typhimurium.
Descritores: Anti-Infecciosos
Bactérias
Bignoniaceae/química
Extratos Vegetais/farmacologia
Psidium/química
Pterocarpus/química
-Anacardiaceae/química
Bacillus cereus
Casca de Planta/química
Etanol/farmacologia
Hexanos/farmacologia
Folhas de Planta/química
Medicina Tradicional
México
Salmonella typhimurium
Staphylococcus aureus
Responsável: CL1.1 - Biblioteca Central


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Id: lil-626749
Autor: Keyhanmanesh, Rana; Boskabady, Mohammad H.
Título: Relaxant effects of different fractions from Tymus vulgaris on guinea-pig tracheal chains
Fonte: Biol. Res;45(1):67-73, 2012. ilus, tab.
Idioma: en.
Resumo: In previous studies, the relaxant effect of Tymus vulgaris has been demonstrated on guinea pig tracheal chains. Therefore, in the present study, the relaxant effects of n-hexane, dichloromethane, methanol and aqueous fractions of Tymus vulgaris on tracheal chains of guinea pigs were examined. The relaxant effects of four cumulative concentrations of each fraction (0.4, 0.8, 1.2 and 1.6 g%) in comparison to saline as negative control and four cumulative concentrations of theophylline (0.2, 0.4, 0.6 and 0.8 mM) were examined for their relaxant effects on precontracted tracheal chains of guinea pig by 60 mM KCl (group 1) and 10 ìÌ methacholine (group 2, n = 7 for each group). In group 1, all concentrations of the n-hexane fraction and theophylline and three last concentrations (0.8, 1.2 and 1.6 g%) of dichloromethane and two higher concentrations (1.2 and 1.6 g%) of methanol fractions showed significant relaxant effects compared to that of saline (p<0.05 to p<0.001). In group 2, all concentrations of theophylline, n-hexane and dichloromethane fractions and three concentrations (0.8, 1.2 and 1.6 g%) of methanol and two higher concentrations (1.2 and 1.6 g%) of aqueous fractions showed significant relaxant effects compared to that of saline (p<0.05 to p<0.001). In addition, with group 1, the relaxant effect of all concentrations of all fractions except the n-hexane fraction, were significantly less than those of theophylline (p<0.05 to p<0.001). The n-hexane fraction showed higher relaxant effect than theophylline. The relaxant effect of all concentrations of the n-hexane fraction and the three last concentrations (0.8, 1.2 and 1.6 g%) of dichloromethane and aqueous fractions were significantly greater in group 2 than in group 1 (p<0.05 to p<0.001). There were significant positive correlations between the relaxant effects and concentrations for theophylline and all fractions (except aqueous fraction in group 1) in both groups, but a negative correlation for the aqueous fraction in group 1 (p<0.05 to p<0.001). These results showed a potent relaxant effect for n-hexane and weaker relaxant effect for other fractions from Tymus vulgaris on tracheal chains of guinea pigs.
Descritores: Broncodilatadores/farmacologia
Hexanos/farmacologia
Cloreto de Metileno/farmacologia
Extratos Vegetais/farmacologia
Thymus (Planta)/química
Traqueia/efeitos dos fármacos
-Metanol/farmacologia
Relaxamento Muscular/efeitos dos fármacos
Músculo Liso/efeitos dos fármacos
Fitoterapia
Óleos Vegetais/farmacologia
Soluções
Teofilina/farmacologia
Água/química
Limites: Animais
Feminino
Cobaias
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: lil-618852
Autor: Pérez Gutierrez, Rosa Martha; Hoyo-Vadillo, Carlos.
Título: Anti-diabetic activity of an hexane extract of Prosthechea michuacana in streptozotocin-induced diabetic rats / Actividad antidiabética de un extracto de hexane de Prosthechea michuacana en ratas diabéticas inducidas por estreptozotocina
Fonte: Bol. latinoam. Caribe plantas med. aromát;10(6):570-580, ene. 2011. tab.
Idioma: en.
Resumo: In this study we investigated the antihyperglycaemic, antihyperlipidaemic and antiglycation effects of some extracts of Prosthechea michuacana bulbs in normoglycemic and diabetic rats induced by streptozocin (STZ). Hexane, chloroform and methanol extracts of P. michuacana were screened for hypoglycemic activity, and biochemical parameters as serum triglycerides, total cholesterol, lipid peroxidation, liver glycogen, skeletal muscle glycogen levels, superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase activity in diabetic rats. Additionally we determined Glucose 6 Phosphatase and glucokinase activities in liver, inhibition of insulin and protein glycation. Glucose levels in blood plasma were determined by using GOD-POD method. Administration of chloroform and methanol extracts showed no effect on STZ induced diabetic rats (SD). On the other hand, treatment with hexane extract at 200 and 400 mg/kg doses, resulted in a reversal of diabetes and its complications. Both doses significantly brought down blood glucose concentration (35.75 and 47.78 percent in diabetic rats, 50.64 and 57.10 percent in nondiabetic rats), increased glycogenesis and decreased glyconeogenesis bringing the glucose metabolism toward normalcy. These doses also reversed the hyperlipidemia by reducing cholesterol (41.56 percent, 46.08 percent) and triglycerides (37.5 percent, 46.27 percent) and improved hepatic antioxidant enzyme activities. Its effect was compared with that of glibenclamide and tolbutamide, as reference antidiabetic drugs. The hexane extract decreased the hyperinsulinemia by 24 percent in SD and showed a significant change on AGEs formation in vitro with IC50 values of 48.3 ug/ml comparable to inhibitory effect of aminoguanidine with IC50 values of 27.2 ug/ml. It reduced HbA1C levels by 6.4 percent in chronic STZ-diabetic rats. It is concluded that hexane extract of Prosthechea michuacana bulbs possesses anti-hyperglycemic and antihyperlipemic...

En este estudio se determinaron los efectos antidiabéticos, antihiperlipidemico y glicación (AGEs) de algunos extractos de Prosthechea michuacana (PM) en ratas normoglucémicas y con diabetes inducida por estreptozotocina (STZ). Se probó el efecto de los extractos de hexano, cloroformo, metanol de PM sobre la actividad hipoglucemiante, la carga de glucosa, los parámetros bioquímicos tales como triglicéridos, niveles de colesterol total, peroxidación lipídica, glucógeno del hígado, los niveles de glucógeno muscular, niveles de superoxide dismutase, catalasa, glutation reductasa and glutation peroxidasa en ratas normales y diabéticas. También se determinó la glucosa 6 Phosphatasa y las actividades de GK en el hígado, la inhibición de la insulina y la glicosilación de las proteínas. Los niveles de glucosa sanguínea se determinaron por el método de GOD-POD. La administración de los extractos de cloroformo y metanol no presentaron ningún efecto sobre la SD, en cambio el tratamiento con el extracto de hexano (PM) a dosis de 200 y 400 mg/kg, inhibió la diabetes y sus complicaciones. Ambas dosis redujeron significativamente los niveles de glucosa sanguínea (35.75 y 47.78 por ciento en las ratas diabéticas, 50.64 y 57.10 por ciento en las ratas diabéticas), el aumento de la glucogénesis y la disminución de la gluconeogénesis conduce el metabolismo de la glucosa hacia la normalidad. Estas dosis disminuyeron la hiperlipidemia reduciendo el colesterol (41.56 por ciento, 46.08 por ciento) y los triglicéridos (37.5 por ciento, 46.27 por ciento) así como también mejoran las actividades antioxidantes de las enzimas hepáticas. Su efecto se comparó con la glibenclamida y tolbutamida, fármacos usados como antidiabeticos. El extracto de hexano disminuyo la hiperinsulinemia en un 24 por ciento en SD. PM mostró un cambio significativo in vitro sobre la formación de los AGEs con valores de IC50 de 48.3 mg/ml comparable al efecto inhibidor de la aminoguanidina con valores de IC50 de...
Descritores: Diabetes Mellitus Experimental/tratamento farmacológico
Extratos Vegetais/farmacologia
Hipoglicemiantes/farmacologia
Orchidaceae/química
-Hexanos/química
Hipolipemiantes/farmacologia
Ratos Wistar
Limites: Masculino
Animais
Ratos
Responsável: CL1.1 - Biblioteca Central



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