Base de dados : LILACS
Pesquisa : D02.455.426.392.368.450 [Categoria DeCS]
Referências encontradas : 11 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 2 ir para página        

  1 / 11 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: lil-657665
Autor: Rivera-Domínguez, Marisela; Astorga-Cienfuegos, Karen Rosalinda; Tiznado-Hernández, Martin Ernesto; González-Aguilar, Gustavo Adolfo.
Título: Induction of the expression of defence genes in Carica papaya fruit by methyl jasmonate and low temperature treatments
Fonte: Electron. j. biotechnol;15(5):6-6, Sept. 2012. ilus, tab.
Idioma: en.
Resumo: The defence mechanisms that are activated by methyl jasmonate (MJ) in fruits are not well understood. In this work, we studied the expression of defence genes in papaya fruit that are induced by the exposure to MJ and/or low temperatures. The papaya fruits 'Maradol' were randomly divided into two groups: one group was the untreated control and the other was treated with 10-4 M of MJ. Half of the fruits from each of the two groups were stored after treatment for 5 days at 5ºC and 2 days at 20ºC. We studied the expression levels of the pdf1.1 and pdf1.2 genes by amplification from expression libraries created from the pulp and skin tissues of the papaya fruit. As a reference, the mRNA level of the 18s ribosomal gene was used. In the skin tissue, the expression levels of the pdf1.1 and pdf1.2 genes were higher immediately after MJ treatment compared to the control. Furthermore, the expression of pdf1.2 remained high after MJ treatment and subsequent storage compared to the control. It was therefore concluded that the activation of the pdf1.1 and pdf1.2 genes forms part of the molecular defence mechanism in fruits that is activated by exposure to MJ. To our knowledge, this is the first study that analyzes the gene expression in papaya fruit that is induced by the exogenous application of methyl jasmonate and cold treatment.
Descritores: Acetatos/farmacologia
Temperatura Baixa
Carica
Carica/genética
Ciclopentanos/farmacologia
-Carica/metabolismo
DNA Complementar
Expressão Gênica
Oxilipinas/farmacologia
Reação em Cadeia da Polimerase
Temperatura
Responsável: CL1.1 - Biblioteca Central


  2 / 11 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: lil-657662
Autor: Shen, Xu; Du, Qingshan; Xu, Yufang; Lu, Yanhua.
Título: Stimulation of trans-resveratrol biosynthesis in Vitis vinifera cv. Kyoho cell suspension cultures by 2, 3-dihydroxypropyl jasmonate elicitation
Fonte: Electron. j. biotechnol;15(5):3-3, Sept. 2012. ilus, tab.
Idioma: en.
Projeto: Fundamental research Funds for the Central Universities; . 863 Project; . Shanghai Leading Academic Discipline Project; . National Special Fund for State Key Laboratory of Bioreactor Engineering.
Resumo: Background: Plant cell suspension culture of Vitis vinifera is a promising technology for investigating different factors that are able to induce and/or modify stilbenes biosynthesis. Jasmonates have been reported to play an important role in a signal transduction pathway that regulates defence responses as well as the production of secondary metabolites. In this study, 2, 3-dihydroxypropyl jasmonate (DHPJA) was used to investigate its effect on stimulating trans-resveratrol (t-R) accumulation and the plant defence responses in Vitis vinifera cv. Kyoho cell suspension cultures for the first time. Results: It demonstrated that DHPJA had superior effects on stilbenoids accumulation over methyl jasmonate (MeJA). The optimal condition was 150 uM DHPJA added on day 15 of cultivation period, with the highest level of t-R accumulation which was increased 1.8-fold and 1.3-fold compared with the control and 150 uM MeJA respectively. DHPJA induced stronger plant defence responses, including oxidative burst and activation of L-phenylalanine ammonia lyase (PAL) than MeJA. H2O2 generation induced by DHPJA played a significant role in enhancing t-R accumulation. Adding a specific inhibitor of H2O2 signalling pathway inhibited DHPJA-induced t-R accumulation, but had no effects on DHPJA-induced other metabolites accumulation, which resulted in regulations of product diversity. Conclusions: This study demonstrated that DHPJA was an efficient elicitor to enhance t-R accumulation by activating stronger oxidative burst, and H2O2 signalling pathway could regulate product diversity in DHPJA-induced V. vinifera cv. Kyoho cell suspension cultures.
Descritores: Ciclopentanos/farmacologia
Estilbenos/metabolismo
Vitis/metabolismo
-Células Cultivadas
Oxilipinas
Responsável: CL1.1 - Biblioteca Central


  3 / 11 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Costa Rica
Texto completo
Id: lil-638106
Autor: Suan See, Koay; Bhatt, Arvind; Lai Keng, Chan.
Título: Effect of sucrose and methyl jasmonate on biomass and anthocyanin production in cell suspension culture of Melastoma malabathricum (Melastomaceae)
Fonte: Rev. biol. trop;59(2):597-606, jun. 2011. ilus.
Idioma: en.
Resumo: Melastoma malabathricum, belongs to the Melastomaceae family, is an important medicinal plant widely distributed from Madagascar to Australia, that is used in traditional remedies for the treatment of variousailments. Besides its medicinal properties, it has been identified as a potential source of anthocyanin production.The present study was carried out to investigate the effect of sucrose and methyl jasmonate and feeding time oncell biomass yield and anthocyanin production in cell suspension culture of M. malabathricum. Addition of differentconcentrations of sucrose into the cell culture of M. malabathricum influenced cell biomass and pigment accumulation. The addition of methyl jasmonate was found to have no effect on cell biomass but the presence of higher amount (12.5-50mg/L) had caused a reduction in anthocyanin production and accumulation. MS medium supplemented with 30g/L sucrose and 3.5 mg/L of MeJA added on cero day and 3rd day produced high fresh cell mass at the end of nine days of culture but did not support the production of anthocyanins. However, cells cultured in the medium supplemented with 45g/L sucrose without MeJA showed the highest pigment content (0.69±0.22Cv/g-FCM). The cells cultured in MS medium supplemented with 30 g/L sucrose with 3.5mg/L MeJA added on the 3rd and 6th day of culture, showed the lowest pigment content (0.37-0.40Cv/g-FCM). This study indicated that MeJA was not necessary but sucrose was needed for the enhancement of cell growth and anthocyanin production in M. malabathricum cell cultures. Rev. Biol. Trop. 59 (2): 597-606. Epub 2011 June 01.

elastoma malabathricum pertenece a la familia de las melastomáceas, es una planta medicinal importante ampliamente distribuida desde Madagascar hasta Australia, que se utiliza en remedios tradicionales para el tratamiento de diversas dolencias. Además de sus propiedades medicinales, se ha identificado como una fuente potencial de producción de antocianinas. En esta investigación se estudió el efecto de la sucrosa, el metil jasmonato y el tiempo de ingestión en la producción de biomasa de las células y la producción de antocianinas, en el cultivo de células en suspensión de M. malabathricum. La adición de diferentes concentraciones de sucrosa al cultivo de células de M. malabathricum influencia la biomasa de las células y la acumulación de pigmento. La adición de metil jasmonato no tuvo ningún efecto sobre la biomasa celular, pero la presencia de una cantidad más alta (12.5-50mg/L) causó una reducción en la producción y acumulación de antocianinas. El medio MS complementado con sucrosa 30g/L y 3.5mg/L de MeJA en el día cero y el tercer día produjo una gran masa de células frescas al final de los nueve días de cultivo pero no se pudo mantener la producción de antocianinas. Sin embargo, las células cultivadas en el medio complementado con 45g/L de sucrosa sin MeJA mostró el mayor contenido de pigmento (0.69±0.22cv/g-fcm). Las células cultivadas en el medio MS complementado con 30 g/L de sucrosa y con 3.5 mg/l MeJA en el tercer y sexto día de cultivo, mostró el menor contenido de pigmentos (0.37-0.40cv/g-fcm). Este estudio indicó que MeJA no era necesario pero la sucrosa sí se necesitaba para mejorar el crecimiento celular y la producción de antocianinas en cultivos de células de M. malabathricum.
Descritores: Acetatos
Antocianinas/biossíntese
Biomassa
Ciclopentanos/farmacologia
Melastomataceae/efeitos dos fármacos
Oxilipinas/farmacologia
Sacarose/farmacologia
-Células Cultivadas
Melastomataceae/crescimento & desenvolvimento
Melastomataceae/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CR1.1 - BINASSS - Biblioteca Nacional de Salud y Seguridad Social


  4 / 11 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-595044
Autor: Barradas-Dermitz, Dulce Ma; Hayward-Jones, Patricia M; Mata-Rosas, Martín; Palmeros-Sánchez, Beatriz; Platas-Barradas, Oscar B. J; Velásquez-Toledo, Rodolfo F.
Título: Taxus globosa S. cell lines: initiation, selection and characterization in terms of growth, and of baccatin III and paclitaxel production
Fonte: Biocell;34(1):1-6, Apr. 2010. tab, ilus.
Idioma: en.
Resumo: Of the initial six cell lines originating from explants of Taxus globosa, or Mexican yew (stem internode, leaves and meristematic tissue), three were selected for their microbial and oxidation resistance, two from leaves and the other from stem internode. A study of their behavior, both in terms of cell growth, and of baccatin III and paclitaxel production, was developed in suspension cultures with an initially standardized biomass (fresh weight 0.23 g/L) using modified Gamborg's B5 medium, and an elicitor (methyl jasmonate), on either the first or seventh day of culture, at several levels (0, 0.1, 1, 10, 100 microM). In most of the conditions used, the three cell lines showed growth associated baccatin III production. The cell line from stem internode was the highest producer of baccatin III using 1 microM elicit or, sampling at 10 days.
Descritores: Acetatos
Alcaloides/biossíntese
Ciclopentanos/farmacologia
Paclitaxel/biossíntese
Proliferação de Células
Taxus/citologia
Taxus
Taxus/metabolismo
-Técnicas de Cultura de Células
Linhagem Celular
Meios de Cultura
Taxoides
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo


  5 / 11 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-595018
Autor: Martin, Maria Teresa; Pedranzani, Hilda; García-Molinero, Patricia; Pando, Valentin; Sierra-de-Grado, Rosario.
Título: Inhibitory effect of jasmonic acid and ethylene on epicotyl growth and bud induction in the maritime pine, Pinus pinaster Soland. in Ait
Fonte: Biocell;33(3):141-148, Dec. 2009. graf, tab.
Idioma: en.
Resumo: Two independent parameters, epicotyl height (cm) and number of induced buds were studied on Pinus pinaster explants to analyse the effects of three phytohormones (6-benzylaminopurine, jasmonic acid, ethylene) which were combined or not in 11 different treatments. Epicotyle length diminished significantly in relation to the control medium (medium without exogen phytohormones) in presence of jasmonic acid, 6-benzylaminopurine or Ethephon (which is converted to ethylene in plants) in any of treatments. Concentrations of 100 microM of jasmonic acid and Ethephon had a greater inhibitory effect than the treatments with 10 microM. In addition to that, jasmonic acid was a stronger inhibitor than Ethephon in any of the tried combinations. There were no significant differences between the control treatment and the treatment s with only 10 microM of jasmonic acid or Ethephon. However, 10 microM 6-benzylaminopurine induced bud formation. The different combinations of 6-benzylaminopurine with jasmonic acid and Ethephon showed that concentrations of 10 to 100 microM did not affect the number of induced buds. Jasmonic acid had an inhibitory effect which Ethephon only showed when combined with 100 microM of jasmonic acid and 10 microM of 6-benzylaminopurine. Three response groups were defined by cluster analysis: group 1 produced the greatest mean number of buds (4 to 5) and a mean epicotyl growth of 1 to 1.5 cm; group 2 produced 2 to 4 buds and a mean growth of 0.5 to 1.2 cm; group 3 produced only one bud and a mean epicotyl length of 1.2 to 2 cm.
Descritores: Ciclopentanos/farmacologia
Compostos Organofosforados/farmacologia
Compostos de Benzil/farmacologia
Etilenos/farmacologia
Etilenos/metabolismo
Purinas/farmacologia
-Componentes Aéreos da Planta/crescimento & desenvolvimento
Componentes Aéreos da Planta
Pinus/crescimento & desenvolvimento
Pinus
Reguladores de Crescimento de Planta/farmacologia
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo


  6 / 11 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: lil-591937
Autor: Carvajal, Marcela; Espinoza, Luis; Caggia, Silvia; Cardile, Venera; Garbarino, Juan A; Peña-Cortés, Hugo; Russo, Alessandra.
Título: New stereoisomeric derivatives of jasmonic acid generated by biotransformation with the fungus Gibberella fujikuroi affect the viability of human cancer cells
Fonte: Electron. j. biotechnol;14(2):7-7, Mar. 2011. ilus, tab.
Idioma: en.
Projeto: MECESUP.
Resumo: Background: Several studies have shown that (-)-Jasmonic acid, (+)-7-iso-Jasmonic acid and its methyl ester, methyl jasmonate, have anti-cancer activity in vitro and in vivo, exhibiting selective cytotoxicity towards cancer cells. The degree of activity of these molecules is strongly related to their stereochemistry. The biotransformation of known compounds, natural or synthesized, related to interesting biological activities, generates new molecules displaying new improved properties compared with the original ones, increasing its value and providing new more effective products. Therefore, based on the above rationales and observations, in this work a biotransformation protocol to modify the chemical structure of the plant hormone jasmonic acid by using the fungus Gibberella fujikuroi was established. Results: The three jasmonic acid derivatives obtained, 3(S)-Hydroxy-2(R)-(2Z-pentenyl)-cyclopentane-1(R)-acetic acid (1), 3(R)-Hydroxy-2(R)-(2Z-pentenyl)-cyclopentane-1(R)-acetic acid (2), 3-Hydroxy-2(S)-(2Z-pentenyl)-cyclopentane-1(S)-acetic acid (3), were tested for cell-growth inhibition activity towards the human cancer epithelial cell line, the oral squamous carcinoma cells (KB). The results obtained show that jasmonic acid derivatives (1-3) are active on human cancer cells examined in different concentration ranges, with IC50 value less than of 25 uM. The compound 3, with the same molecular structure of compounds 1 and 2, but with different stereochemistry, was more active confirming that the activity of jasmonate compounds is related to their stereochemistry and to substituents in the cyclopentane ring. In this study, we also tested the potential proapoptotic activity of compound 3, and our data suggest that it, as other jasmonate compounds, is able to trigger apoptotic death in cancer cells. This event may be correlated at an elevation of reactive oxygen species (ROS). Administration of N-acetylcysteine (NAC) prevented compound 3 cytotoxicity...
Descritores: Apoptose
Ciclopentanos/metabolismo
Gibberella/metabolismo
Oxilipinas/metabolismo
-Antineoplásicos
Bioensaio
Biotransformação
Sobrevivência Celular
Ensaio Cometa
Espécies Reativas de Oxigênio
L-Lactato Desidrogenase
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


  7 / 11 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-548248
Autor: Pereira Lopes, JEF; Barbosa, MR; Stella, CN; Santos, WA; Pereira, EM; Nogueira-Neto, J; Augusto, EM; Silva, LV; Smaili, SS; Gomes, LF.
Título: In vivo anti-angiogenic effects further support the promise of the antineoplasic activity of methyl jasmonate / Efeitos antiangiogênicos in vivo convalidam a atividade antineoplásica potencial do metiljasmonato
Fonte: Braz. j. biol;70(2):443-449, May 2010. ilus, graf, tab.
Idioma: en.
Resumo: Molecular plant components have long been aimed at the angiogenesis and anti-angiogenesis pathways, and have been tested as sources for antineoplasic drugs with promising success. The present work deals with the anti-angiogenic effects of Methyl Jasmonate. Jasmonate derivatives were demonstrated to selectively damage the mitochondria of cancer cells. In vitro, 1-10 mM Methyl Jasmonate induced the cell death of the human umbilical vein endothelial cells (HUVEC) and the Murine melanoma cells (B16F10), while micromolar concentrations were ineffective. In vivo, comparable concentrations were toxic and reduced the vessel density of the Chorioallantoic Membrane of the Chicken Embryo (CAM). However, 1-10 µM concentrations produced a complex effect. There was increased capillary budding, but the new vessels were leakier and less organised than corresponding controls. It is suggested that not only direct toxicity, but also the drug effects upon angiogenesis are relevant to the antineoplasic effects of Methyl Jasmonate.

Moléculas de origem vegetal são, há muito, conhecidas como substâncias ativas sobre as vias de angiogênese e antiangiogênese e foram testadas como fonte de drogas antineoplásicas com sucesso promissor. Este trabalho trata dos efeitos antiangiogênicos do Metiljasmonato, um protótipo da família dos derivados do ácido jasmônico, que danificam seletivamente a mitocôndria de células neoplásicas. In vitro, metiljasmonato 1-10 mM promoveu a morte celular de células endoteliais humanas de cordão umbilical (HUVEC) e de melanoma murino (B16F10); concentrações micromolares foram inócuas. In vivo, concentrações equivalentes foram tóxicas e reduziram a densidade de vasos em membranas corioalantoicas de embrião de galinha (CAM). Entretanto, concentrações entre 1-10 µM produziram um efeito complexo. Ocorreu aumento no brotamento capilar, mas os novos vasos apresentaram-se frágeis e menos organizados que os controles correspondentes. Sugere-se que, além da toxicidade direta contra as células tumorais, a ação do metiljasmonato sobre a angiogênese seja relevante para seu efeito antineoplásico.
Descritores: Acetatos
Inibidores da Angiogênese/farmacologia
Antineoplásicos/farmacologia
Membrana Corioalantoide/efeitos dos fármacos
Ciclopentanos/farmacologia
Células Endoteliais/efeitos dos fármacos
Oxilipinas/farmacologia
-Linhagem Celular Tumoral
Membrana Corioalantoide/irrigação sanguínea
Células Endoteliais/citologia
Veias Umbilicais/citologia
Veias Umbilicais/efeitos dos fármacos
Limites: Animais
Embrião de Galinha
Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  8 / 11 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: lil-448793
Autor: Hernández, Xenia E; Orden, Alejandro A; Giordano, Oscar S; Kurina, Marcela.
Título: Effects of elicitor and copper sulfate on grindelic acid production in submerged cultures of Grindelia pulchella
Fonte: Electron. j. biotechnol;8(3), Dec. 2005.
Idioma: en.
Projeto: UNSL; . CONICET; . FONCyT; . ANPCyT.
Resumo: Grindelia pulchella callus and cell suspension cultures were established from seedling leaves. When several phytoregulator supplementations were assayed in solid Murashige and Skoog medium containing 3 percent (w/v) of sucrose (MS medium), combinations of indole-3-butyric acid (IBA) and N6-benzylaminopurine (BA) resulted the most appropriate conditions to generate fast growing friable calli with detectable levels of grindelic acid. Moreover, the same basal media supplemented with 20.0 µM IBA/4.4 µM BA was found to be optimal for cell growth in submerged cultures (µmax = 0.26 days-1) while the addition of 20.0 µM IBA/18.0 µM BA resulted in a relative higher metabolite production (4.55 mg/gDW) when the inocula was 5 percent (v/v). Furthermore, three different stress factors and combinations of them were used to elicit cell suspensions. These experiments demonstrated that the combination of CuSO4 and dimethylsulfoxide (DMSO) increase the grindelic acid production to 2.63 mg/gDW in the elicited essay versus 0.756 mg/gDW in the control, at expense of cell growth. In contrast, the addition of jasmonic acid (JA) alone and combined with DMSO neither affected cell growth nor grindelic acid accumulation.
Descritores: Ciclopentanos/metabolismo
Dimetil Sulfóxido/metabolismo
Diterpenos/química
Sulfato de Cobre/metabolismo
-Ácidos Graxos Insaturados/metabolismo
Células Cultivadas
Diterpenos/isolamento & purificação
Responsável: CL1.1 - Biblioteca Central


  9 / 11 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-384258
Autor: Vigliocco, A; Bonamico, B; Alemano, S; Miersch, O; Abdala, G.
Título: Stimulation of jasmonic acid production in Zea mays L. infected by the maize rough dwarf virus-Río Cuarto: reversion of symptoms by salicylic acid
Fonte: Biocell;26(3):369-374, Dec. 2002.
Idioma: en.
Resumo: In the present paper we study the possible biological relevance of endogenous jasmonic acid (JA) and exogenous salicylic acid (SA) in a plant-microbial system maize-virus. The virus disease "Mal de Río Cuarto" is caused by the maize rough dwarf virus-Río Cuarto. The characteristic symptoms are the appearance of galls or enations in leaves, shortening of the stem internodes, poor radical system and general stunting. Changes in JA and protein pattern in maize control and infected plants of a virus-tolerant cultivar were investigated. Healthy and infected-leaf discs were collected for JA measurement at different post-infection times (20, 40, 60 and 68 days). JA was also measured in roots on day 60 after infection. For SDS-PAGE protein analysis, leaf discs were also harvested on day 60 after infection. Infected leaves showed higher levels of JA than healthy leaves, and the rise in endogenous JA coincided with the enation formation. The soluble protein amount did not show differences between infected and healthy leaves; moreover, no difference in the expression of soluble protein was revealed by SDS-PAGE. Our results show that the octadecanoid pathway was stimulated in leaves and roots of the tolerant maize cultivar when infected by this virus. This finding, together with fewer plants with the disease symptoms, suggest that higher foliar and roots JA content may be related to disease tolerance. SA exogenous treatment caused the reversion of the dwarfism symptom.
Descritores: Ácido Salicílico/farmacologia
Ciclopentanos/metabolismo
Doenças das Plantas/virologia
Vírus de Plantas/fisiologia
Zea mays/metabolismo
Zea mays/virologia
-Infecções por Vírus de DNA
Vírus de DNA/fisiologia
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/metabolismo
Folhas de Planta/virologia
Zea mays/efeitos dos fármacos
Zea mays/crescimento & desenvolvimento
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  10 / 11 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Id: lil-335990
Autor: Maccioni, Hugo J. F; Fritz, Victor Rosales; Maxzúd, Mariana K; Daniotti, José L; Martina, José A.
Título: Compartmental organization of ganglioside synthesis in the Golgi complex
Fonte: Biocell;20(3):279-286, Dec. 1996.
Idioma: en.
Descritores: Gangliosídeos/biossíntese
Complexo de Golgi
Vesículas Revestidas/fisiologia
-Brefeldina A
Compartimento Celular
Fracionamento Celular
Ciclopentanos
Complexo de Golgi
Ionóforos/farmacologia
Monensin
Proteínas de Membrana/metabolismo
Limites: Animais
Embrião de Galinha
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



página 1 de 2 ir para página        
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde