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Id: biblio-1010415
Autor: San Pedro, Tània; Peiró, Rosa; Villanova, Joan; Olmos, Antonio; Gisbert, Carmina.
Título: In vitro propagation of Vitis vinifera L. cv. 'Monastrell'
Fonte: Electron. j. biotechnol;27:80-83, May. 2017. ilus, graf.
Idioma: en.
Projeto: FEDER Funds.
Resumo: Background: A protocol for the micropropagation of the grape (Vitis vinifera L.) cultivar 'Monastrell' was developed. Initial plant material was obtained from the sanitary selection of grapevine plants performed by real-time RT-PCR to confirm the absence of Grapevine fanleaf virus, Arabis mosaic virus, Grapevine leafroll-associated virus 1, Grapevine leafroll-associated virus 3, and Grapevine fleck virus. Results: The effects of the salt composition (comparing Lloyd and McCown woody plant medium and Murashige and Skoog medium 1/2 macronutrients) and the growth regulator benzylaminopurine (BAP), at 0 and 8.9 µM, on plant propagation were evaluated using nodes as explants. The most efficient procedure consisted of bud induction in the medium with Lloyd and McCown woody plant salts and 8.9 µM BAP for 30 d along with elongation in cytokinin-free medium for 60 d, which gave 22 nodes/explant (174 plants/initial plant). A second cycle of propagation in a medium without BAP for another 60 d could give approximately 10,000 nodes, which can be obtained after an additional 2 months of culture. All plants acclimatized after the second cycle of multiplication were successfully transferred to soil. Conclusion: We developed an optimal protocol for V. vinifera cv. 'Monastrell' micropropagation, the first described for this cultivar.
Descritores: Vitis/crescimento & desenvolvimento
-Purinas/metabolismo
Compostos de Benzil/metabolismo
Técnicas In Vitro
Cloreto de Sódio/metabolismo
Vitis/virologia
Reação em Cadeia da Polimerase em Tempo Real
Aclimatação
Responsável: CL1.1 - Biblioteca Central


  2 / 14 LILACS  
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Id: lil-795822
Autor: Brazão, Maria AngéLica B; Brazão, Fabio V; Maia, José Guilherme S; Monteiro, Marta C.
Título: Antibacterial activity of the Piper aduncum oil and dillapiole, its main constituent, against multidrug-resistant strains / Actividad antibacteriana del aceite de Piper aduncum y dillapiole, su componente principal, frente a cepas resistentes a múltiples fármacos
Fonte: Bol. latinoam. Caribe plantas med. aromát;13(6):517-526, nov.2014. ilus, tab.
Idioma: en.
Resumo: The study aimed to evaluate the bactericidal activity of oil essential and dillapiole from P. aduncum against standard and multidrug-resistant strains of Staphylococcus spp. The oil showed antimicrobial action against these strains, but better results were obtained for the standards strains of S. epidermidis and S. aureus, with MIC of 250 and 500 ug/mL, respectively. Dillapiolle was less effective than the oil against the same standard and multi-drug resistant strains (MIC =1000 ug/mL). However, when dillapiolle was tested in combination with myristicin, another component of the oil, it increased its bactericidal activity and showed a synergistic action...

El objetivo del estudio fue evaluar la actividad bactericida de los aceites esenciales y dillapiole de P. aduncum contra cepas estándar y multirresistentes de Staphylococcus spp. El aceite mostró acción antimicrobiana frente a estas cepas, pero se obtuvo mejores resultados para las cepas de S. epidermidis y S. aureus, con MIC de 250 y 500 ug/ml, respectivamente. Dillapiolle fue menos eficaz que el aceite contra cepas estándar y multirresistentes (MIC = 1000 ug/ml). Sin embargo, cuando dillapiolle fue probado en combinación con la miristicina, otro componente del aceite, que aumentó su actividad bactericida y mostró una acción sinérgica...
Descritores: Óleos Voláteis/farmacologia
Antibacterianos/farmacologia
Extratos Vegetais/farmacologia
Piper/química
-Compostos Alílicos/farmacologia
Compostos de Benzil/farmacologia
Farmacorresistência Fúngica Múltipla
Monoterpenos/farmacologia
Staphylococcus
Limites: Seres Humanos
Responsável: CL1.1 - Biblioteca Central


  3 / 14 LILACS  
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Texto completo SciELO Brasil
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Id: lil-727022
Autor: Ribeiro, Joyce Benzaquem; Ramos, Aline de Souza; Lopes, Raquel de Oliveira; Silva, Gabriela Veloso Vieira da; Souza, Rodrigo Octavio Mendonça Alves de.
Título: Whole cells in enantioselective reduction of benzyl acetoacetate
Fonte: Braz. j. microbiol;45(3):929-932, July-Sept. 2014. ilus, tab.
Idioma: en.
Resumo: The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after 2 cycles of reaction.
Descritores: Acetoacetatos/metabolismo
Compostos de Benzil/metabolismo
Kluyveromyces/metabolismo
Pichia/metabolismo
-Células Imobilizadas/metabolismo
Oxirredução
Fatores de Tempo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  4 / 14 LILACS  
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Id: lil-672657
Autor: Williams, LAD.
Título: Life's immunity as a normal distribution function: philosophies for the use of dibenzyl trisulphide in immunity enhancement and life extension
Fonte: West Indian med. j;59(5):455-455, Oct. 2010.
Idioma: en.
Descritores: Antioxidantes/farmacologia
Compostos de Benzil/farmacologia
Sulfetos/farmacologia
Timo/efeitos dos fármacos
-Envelhecimento/efeitos dos fármacos
Timo/imunologia
Limites: Seres Humanos
Tipo de Publ: Editorial
Responsável: BR1.1 - BIREME


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Id: lil-672511
Autor: Williams, LAD; Barton, EN; Kraus, W; Rösner, H.
Título: Implications of dibenzyl trisulphide for disease treatment based on its mode of action / Implicaciones del trisulfuro de dibencilo para el tratamiento de enfermedades a partir de su modo de acción
Fonte: West Indian med. j;58(5):407-409, Nov. 2009.
Idioma: en.
Resumo: Studies conducted on the secondary metabolite (natural product), dibenzyl trisulphide (DTS), which was isolated from the sub-tropical shrub Petiveria alliacea (guinea hen weed, anamu) [Phytolaccaceae] have shown tremendous pharmaceutical promise as a drug prototype. This is now reflected in the development of the broad spectrum anti-cancer molecule, fluorapacin (bis(4-fluorobenzyl) trisulphide) which has an excellent safety profile. The mode of action elucidated for DTS is the mitogen activated protein extracellular regulated kinases 1 and 2 (MAPKinases ERK 1 and ERK 2). The MAPKinase signal transduction biochemical pathways are important in the regulation of a wide range of cellular processes which are important in disease establishment. These processes include: cancer cell proliferation, nerve repair, memory enhancement, autoimmune diseases, which are linked to thymic cell involution and bone marrow functions, cerebrovascular and cardiovascular diseases. In addition to the MAPkinase signal transduction mode of action, DTS also prevents the denaturation of serum albumin which is a feature of nonsteroidal anti-inflammatory drugs, thus supporting the molecule's possible role in the treatment of inflammatory ageing diseases.

Los estudios realizados sobre el metabolito secundario (producto natural), trisulfuro de dibencilo (TSD), que fue aislado del arbusto subtropical Petiveria alliacea (hierba de guinea, anamú) [Phytolaccaceae] muestran que se trata de una tremenda promesa farmacéutica como prototipo de droga. Esto se refleja actualmente en el desarrollo de la molécula anticancerígena de amplio espectro, la fluorapacina (bis (4-fluorobencilo) trisulfuro) que posee un excelente perfil de seguridad. El modo de acción para el TSD se explica partiendo de las proteínas quinasas 1 y 2 activadas por mitógenos y reguladas por señales extracelulares (Quinasas MAP ERK 1 y ERK 2). Las vías bioquímicas de transducción de la señal de la quinasa MAP, son importantes en la regulación de una amplia variedad de procesos celulares, importantes a la hora de determinar una enfermedad. Dichos procesos comprenden: la proliferación de la célula cancerosa, la reparación de nervios, el mejoramiento de la memoria, y las enfermedades autoinmunes, vinculadas con la involución tímica y las funciones de la médula, las enfermedades cerebrovasculares y cardiovasculares. Además del modo de acción de las transducción de señales de la quinasa MAP, el TSD previene también la desnaturalización de la albúmina sérica, lo cual constituye una característica de las drogas anti-inflamatorias no esferoidales, apoyando así el posible papel de las moléculas en el tratamiento de las enfermedades inflamatorias en el proceso de envejecimiento.
Descritores: Compostos de Benzil/farmacologia
Desenho de Drogas
MAP Quinases Reguladas por Sinal Extracelular/efeitos dos fármacos
Fluorbenzenos/farmacologia
Phytolaccaceae
Transdução de Sinais/efeitos dos fármacos
Sulfetos/farmacologia
-Diferenciação Celular/efeitos dos fármacos
Proliferação Celular/efeitos da radiação
Células Mesenquimais Estromais/efeitos dos fármacos
Degeneração Neural/tratamento farmacológico
Extratos Vegetais/farmacologia
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: lil-648571
Autor: Mahesh, Ayyavu; Thangadurai, Devarajan; Melchias, Gabriel.
Título: Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze
Fonte: Biol. Res;45(2):131-136, 2012. ilus, tab.
Idioma: en.
Resumo: An efficient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortified with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of high frequency microshoots in 30 days. The microshoots were successfully subcultured for shoot elongation and eventually for rooting on MS medium supplemented with indole-3-butyric acid (IBA) at 0.5mg/l. The regenerated plantlets were hardened under greenhouse conditions and transferred to garden, resulting in a 90% survival rate.
Descritores: Asteraceae/crescimento & desenvolvimento
Organogênese Vegetal/fisiologia
Reguladores de Crescimento de Planta/farmacologia
Folhas de Planta/crescimento & desenvolvimento
Brotos de Planta/crescimento & desenvolvimento
Regeneração/fisiologia
-Asteraceae/efeitos dos fármacos
Compostos de Benzil/farmacologia
Ácidos Naftalenoacéticos
Organogênese Vegetal/efeitos dos fármacos
Folhas de Planta/efeitos dos fármacos
Brotos de Planta/efeitos dos fármacos
Purinas/farmacologia
Regeneração/efeitos dos fármacos
Responsável: CL1.1 - Biblioteca Central


  7 / 14 LILACS  
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Id: lil-595018
Autor: Martin, Maria Teresa; Pedranzani, Hilda; García-Molinero, Patricia; Pando, Valentin; Sierra-de-Grado, Rosario.
Título: Inhibitory effect of jasmonic acid and ethylene on epicotyl growth and bud induction in the maritime pine, Pinus pinaster Soland. in Ait
Fonte: Biocell;33(3):141-148, Dec. 2009. graf, tab.
Idioma: en.
Resumo: Two independent parameters, epicotyl height (cm) and number of induced buds were studied on Pinus pinaster explants to analyse the effects of three phytohormones (6-benzylaminopurine, jasmonic acid, ethylene) which were combined or not in 11 different treatments. Epicotyle length diminished significantly in relation to the control medium (medium without exogen phytohormones) in presence of jasmonic acid, 6-benzylaminopurine or Ethephon (which is converted to ethylene in plants) in any of treatments. Concentrations of 100 microM of jasmonic acid and Ethephon had a greater inhibitory effect than the treatments with 10 microM. In addition to that, jasmonic acid was a stronger inhibitor than Ethephon in any of the tried combinations. There were no significant differences between the control treatment and the treatment s with only 10 microM of jasmonic acid or Ethephon. However, 10 microM 6-benzylaminopurine induced bud formation. The different combinations of 6-benzylaminopurine with jasmonic acid and Ethephon showed that concentrations of 10 to 100 microM did not affect the number of induced buds. Jasmonic acid had an inhibitory effect which Ethephon only showed when combined with 100 microM of jasmonic acid and 10 microM of 6-benzylaminopurine. Three response groups were defined by cluster analysis: group 1 produced the greatest mean number of buds (4 to 5) and a mean epicotyl growth of 1 to 1.5 cm; group 2 produced 2 to 4 buds and a mean growth of 0.5 to 1.2 cm; group 3 produced only one bud and a mean epicotyl length of 1.2 to 2 cm.
Descritores: Ciclopentanos/farmacologia
Compostos Organofosforados/farmacologia
Compostos de Benzil/farmacologia
Etilenos/farmacologia
Etilenos/metabolismo
Purinas/farmacologia
-Componentes Aéreos da Planta/crescimento & desenvolvimento
Componentes Aéreos da Planta
Pinus/crescimento & desenvolvimento
Pinus
Reguladores de Crescimento de Planta/farmacologia
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo


  8 / 14 LILACS  
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Id: lil-471843
Autor: Williams, L. A; Rosner, H; Levy, H. G; Barton, E. N.
Título: A critical review of the therapeutic potential of dibenzyl trisulphide isolated from Petiveria alliacea L (guinea hen weed, anamu)
Fonte: West Indian med. j;56(1):17-21, Jan. 2007.
Idioma: en.
Resumo: The data compiled in the present review on dibenzyl trisulphide (DTS) isolated from Petiveria alliacea L (the guinea hen weed or anamu) revealed that the compound and its derivatives could be of tremendous pharmaceutical interest. The mode of action elucidated for DTS revealed that it is a mitogen activated protein extracellular regulated kinases 1 and 2 (MAPKinases erk1 and erk 2) signal transduction molecule. Dibenzyl trisulphide caused hyper-phosphorylation of growth factor induced MAPKinases (erk 1 and erk 2) phosphorylation, a process critical for the improvement of long term memory, and is implicated in neuronal growth. Dibenzyl trisulphide and its derivatives exhibited potent anti-proliferation/cytotoxic activity on a wide range of cancer cell lines. The cytotoxic activity of DTS was increased by 70-1000 fold when bound to albumin in vitro. Dibenzyl trisulphide seems to have a cytokine switching mechanism in which it down regulates cytokines from the Type I helper cells (Th -1 cell) pathway which contained several pro-inflammatory cytokines and up-regulates those on the Type 2 helper cells (Th-2) pathway. The trisulphide up-regulates some reticuloendothelial system parameters eg granulocyte counts and increased thymic and Peyer's patches masses via cell proliferation processes which are known to be regulated via the MAPKinase signal transduction pathway. When the zygotes ofAsternia pectinifera (Starfish) were exposed to DTS at concentration of 10 mM, a dose lethal to all cancer cells tested, it was observed that the sensitive process of protein biosynthesis was not affected Similarly, the proliferation of the HOFA human fibroblast, a noncancerous cell line, was not severely affected by DTS at 8.9 microM over seven days, a concentration also lethal to most cancer cell lines tested The implications of the findings will be highlighted in the present review.
Descritores: Compostos de Benzil/uso terapêutico
Fitoterapia
Extratos Vegetais
Sulfetos/uso terapêutico
-Antígenos CD/fisiologia
Caderinas/fisiologia
Compostos de Benzil/farmacologia
Regulação para Cima/fisiologia
Sulfetos/farmacologia
Transdução de Sinais/efeitos dos fármacos
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


  9 / 14 LILACS  
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Id: lil-410433
Autor: Williams, L. A; Rõsner, H; Mõller, W; Conrad, J; Nkurunziza, J. P; Kraus, W.
Título: In vitro anti-proliferation/cytotoxic activity of sixty natural products on the human SH-SY5Y neuroblastoma cells with specific reference to dibenzyl trisulphide
Fonte: West Indian med. j;53(4):208-219, Sept. 2004.
Idioma: en.
Resumo: Sixty natural products belonging to the following structural classes: artemisinins, coumarins, flavonoids, tannins, tetrahydroberberine alkaloids, tetracyclic triterpenes, tetranortriterpenoids and polysulphides were screened against the human SH-SY5Y neuroblastoma cell line revealing differences in their effects on cell morphology and in anti-proliferation/cytotoxic activity. Based on the data obtained, dibenzyl trisulphide is the most effective anti-proliferative/cytotoxic compound. In addition, we hereby propose the human SH-SY5Y cell line as a sensitive and uncomplicated in vitro test system for detecting compounds with potential anti-proliferation/cytotoxic activity
Descritores: Antineoplásicos/farmacologia
Compostos de Benzil/farmacologia
Extratos Vegetais/farmacologia
Neuroblastoma/patologia
Sulfetos/farmacologia
-Linhagem Celular Tumoral
Ensaios de Seleção de Medicamentos Antitumorais
Divisão Celular/efeitos dos fármacos
Extratos Vegetais/química
Sobrevivência Celular/efeitos dos fármacos
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


  10 / 14 LILACS  
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Id: lil-405199
Autor: Maseda, Pablo Horacio; Lemcoff, Jorge Hugo; Murúa, Mercedes; Frayssinet, Nora; Carceller, Marta Susana.
Título: Microcutting culture and morpho-physiological changes during acclimation in two Lycium chilense cytotypes
Fonte: Biocell;28(3):271-277, dic. 2004. ilus, tab.
Idioma: en.
Resumo: Lycium chilense, a deciduous perennial shrub, is one of the endangered native species of Patagonia due to sheep overgrazing. Chances of recolonization by seeds are scarce due to the limited density of propagules in the soil and very specific requirements for germination. The objective was to develop an in vitro propagation protocol that would help to perform reestablishment of this species in degraded areas of the Patagonian steppe. Seeds came from two provenances with different somatic number due to differences in ploidy level. Defoliated microcuttings were planted in test tubes with different growing media and taken to a growth chamber. Rooting percentage did not differ between origins, but higher values were encountered for medium without hormones. Subcultures increased significantly rooting percentage and reduced time to rooting. The leaves from micropropagated plants were thinner, did not exhibit hairs, and had poorly developed palisade parenchyma and less epicuticular waxes. In vitro leaves had lower stomatal density and their stomata were less functional when compared to acclimated leaves. A repopulation program of Lycium chilense based on microcutting culture, specialy using subcultures, is feasible.
Descritores: Aclimatação/fisiologia
Técnicas de Cultura de Células
Lycium
Lycium/genética
Morfogênese/fisiologia
-Compostos de Benzil/farmacologia
Folhas de Planta/anatomia & histologia
Folhas de Planta/crescimento & desenvolvimento
Folhas de Planta/ultraestrutura
Ácidos Indolacéticos
Lycium/crescimento & desenvolvimento
Microscopia Eletrônica de Varredura
Ácidos Naftalenoacéticos
Raízes de Plantas
Ploidias
Purinas/farmacologia
Reguladores de Crescimento de Planta/farmacologia
Caules de Planta/crescimento & desenvolvimento
Caules de Planta
Responsável: AR1.1 - Biblioteca Rafael Herrera Vegas



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