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Id: biblio-837694
Autor: Barros, Pedro Paulo; Gonçalves, Gisele Mara Silva; Silva, Gustavo Henrique da; Bastos, Maria Clara Villaça Dias; Ramos, Loren Nogaroto; Fernandes, Marilia Marinello.
Título: Lycopene and resveratrol pretreatment did not interfere with the liver of hepatectomized rats
Fonte: Acta cir. bras;32(3):194-202, Mar. 2017. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To investigate the effects of lycopene and resveratrol pretreatment on hepatic hyperplasia in partially hepatectomized rats. Methods: The lycopene group and the resveratrol group received 40 mg/kg/day of lycopene or resveratrol, respectively (dissolved in olive oil or in saline solution, respectively) and administered via a gastric tube for 30 days. The partially hepatectomzed (PH) control groups received saline or olive oil via a gastric tube for 30 days, respectively, and the normal control group received no treatment. Liver tissue and intracardiac blood samples were obtained 24, 36 or 48 h after PH. Results: No areas of fibrosis were detected. No significant changes in mitotic index, in the number of apoptosis events or in aspartate aminotransferase and alanine aminotransferase levels were observed. Conclusions: Lycopene and resveratrol pretreatment did not interfere on hepatic hyperplasia in partially hepatectomized rats.
Descritores: Estilbenos/farmacologia
Carotenoides/farmacologia
Hepatectomia/métodos
Fígado/cirurgia
Fígado/efeitos dos fármacos
Antioxidantes/farmacologia
-Aspartato Aminotransferases/sangue
Fatores de Tempo
Reprodutibilidade dos Testes
Resultado do Tratamento
Ratos Wistar
Apoptose/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Alanina Transaminase/sangue
Fígado/enzimologia
Fígado/patologia
Regeneração Hepática/efeitos dos fármacos
Índice Mitótico
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-1008576
Autor: Deng, Nan; Liu, Caixia; Chang, Ermei; Ji, Jing; Yao, Xiamei; Yue, Jianyun; Bartish, Igor V; Chen, Lanzhen; Jiang, Zeping; Shi, Shengqing.
Título: High temperature and UV-C treatments affect stilbenoid accumulation and related gene expression levels in Gnetum parvifolium
Fonte: Electron. j. biotechnol;25:43-49, ene. 2017. ilus, tab, graf.
Idioma: en.
Projeto: State Key Laboratory of Tree Genetics and Breeding; . 948 project; . Fund of National Non-profit Research Institutions of CAF.
Resumo: Background: Gnetum parvifolium stems and roots have been used for a long time in traditional Chinese medicines. Stilbenes are bioactive compounds present in G. parvifolium plants, and they possess antioxidative and anticancer properties. However, little is known about the responses of G. parvifolium stilbene biosynthetic pathways to stress conditions. Therefore, we investigated stilbene biosynthesis, including the expression of relevant genes, in G. parvifolium exposed to high-temperature and ultraviolet-C treatments. Results: High temperatures did not influence the accumulation of total stilbenes in stems but decreased stilbene concentrations in roots at 3 h, with a subsequent restoration to control levels. In contrast, ultraviolet irradiation induced the accumulation of total stilbenes in stems but not in roots. We also observed that high temperatures inhibited the production of resveratrol and piceatannol in G. parvifolium stems and roots, whereas ultraviolet treatments initially inhibited their accumulation (up to 6 h) but induced their production at later time points. Analyses of specific genes (i.e., PAL, C4H, 4CL, STS, and CYP) revealed that their expression levels generally increased in stress-treated stems and roots, although there was some variability in the expression profiles during treatments. Conclusions: Our results indicated that high temperatures and ultraviolet irradiation differentially affect the biosynthesis of specific stilbenes in G. parvifolium stems and roots. Therefore, cultivating G. parvifolium seedlings under optimal stress conditions may increase the biosynthesis of specific stilbene compounds.
Descritores: Estilbenos/análise
Gnetum/metabolismo
-Estilbenos/efeitos da radiação
Estilbenos/metabolismo
Raios Ultravioleta
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Gnetum/efeitos da radiação
Gnetum/genética
Plântula
Polifenóis/análise
Polifenóis/efeitos da radiação
Polifenóis/metabolismo
CHLOROFLUOROCARBONS, METHANE1
Temperatura Alta
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-893150
Autor: Jalili, Cyrus; Makalani, Fatemeh; Roshankhah, Shiva; Sohrabi, Khadije; Salahshoor, Mohammad Reza.
Título: Protective effect of resveratrol against morphine damage to kidneys of mice / Efecto protector de resveratrol contra el daño de la morfina en riñones de ratones
Fonte: Int. j. morphol;35(4):1409-1415, Dec. 2017. graf.
Idioma: en.
Projeto: Kermanshah University of Medical Sciences.
Resumo: SUMMARY: Morphine produces free radicals and cause apoptosis in some cell. Resveratrol (RSV) is a stilbenoid, a type of natural phenol, and a phytoalexin produced by several plants in response to injury. 48 male mice were randomly assigned to 8 groups. In this study, various doses of RSV (2, 8 and 20 mg/kg) and RSV plus Morphine (2, 8 and 20 mg/kg) were administered intraperitoneally to male mice for 20 consequent days and weight of kidneys, biochemical characteristics, morphometric markers and blood serum nitric oxide level were studied. The results indicated that morphine administration significantly increased the mean diameter of glomerulus and distal and proximal convoluted tubule, Lactate dehydrogenase (LDH), Blood urea nitrogen (BUN), creatinine and nitric oxide levels compared to the saline group (P<0.05). However, RSV and RSV plus morphine in all doses significantly decreased glomeruli number and LDH, BUN, creatinine and nitric oxide levels compared to morphine groups (p<0.05). Thus, it seems that resveratrol improved kidney damages induced by morphine in mice.

RESUMEN: La morfina produce radicales libres y causa apoptosis en algunas células. El resveratrol (RSV) es un tipo de fenol natural y una fitoalexina producida por varias plantas en respuesta a una lesión. Se asignaron al azar 48 ratones machos a 8 grupos. En este estudio se administraron varias dosis de RSV (2, 8 y 20 mg/kg) y RSV más morfina (2, 8 y 20 mg/kg) intraperitoneal en ratones machos durante 20 días consecutivos y se estudió el peso de los riñones, las características bioquímicas, los marcadores morfométricos y el nivel de óxido nítrico en suero sanguíneo. Los resultados indicaron que la administración de morfina aumentó significativamente el diámetro medio del glomérulo y de los túbulos distal y proximal, los niveles de lactato deshidrogenasa (LDH), nitrógeno ureico en sangre (BUN), la creatinina y el óxido nítrico en comparación con el grupo salino (p <0,05). Sin embargo, el RSV y el RSV más morfina en todas las dosis redujeron significativamente el número de glomérulos y LDH, BUN, la creatinina y el óxido nítrico en comparación con los grupos de morfina (p <0,05). Por lo tanto, los resultados podrían indicar que el resveratrol mejoró el daño renal inducido por la morfina en ratones.
Descritores: Estilbenos/administração & dosagem
Rim/efeitos dos fármacos
Morfina/toxicidade
-Creatinina/sangue
Glomérulos Renais/efeitos dos fármacos
Camundongos Endogâmicos BALB C
Óxido Nítrico/sangue
Limites: Animais
Masculino
Camundongos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-893139
Autor: Jalili, Cyrus; Salahshoor, Mohammad Reza; Jalili, Faramarz; Kakabaraei, Seyran; Akrami, Ali; Sohrabi, Maryam; Ahookhash, Maryam; Ghanbari, Ali.
Título: Therapeutic effect of resveratrol on morphine-induced damage in male reproductive system of mice by reducing nitric oxide serum level / Efecto terapéutico de resveratrol sobre el daño inducido por la morfina en el sistema reproductor masculino de ratones reduciendo el nivel de óxido nítrico del suero
Fonte: Int. j. morphol;35(4):1342-1347, Dec. 2017. tab, graf.
Idioma: en.
Projeto: Kermanshah University of Medical Sciences.
Resumo: SUMMARY: Morphine is one of the naturally occurring phenanthrene alkaloids of opium that induces adverse effects on male reproductive system. Resveratrol is a phytoestrogen and antioxidant of red grape. The main goal is to investigate whether resveratrol could inhibit adverse effects of morphine on sperm cell viability, count, motility as well as testis histology, testosterone hormone and nitric oxide levels in mice. In the present study, 48 male rats were randomly divided into 8 groups (n=6) and were treated intraperitoneally for 14 days with normal saline, resveratrol (2, 8, 20 mg/kg/day), morphine (20 mg/kg/day) and morphine (20 mg/kg/day) + resveratrol (2, 8, 20 mg/kg/day). At the end of experiments, sperm parameters (sperm cell viability, count, motility and morphology), testis weight, the diameter of seminiferous tubules, testosterone hormone level and nitric oxide were analyzed. The data were analyzed by SPSS software for windows (version 20) using one-way ANOVA test followed by Tukey's post hoc test, and P<0.05 was considered significant. The results indicated that morphine administration significantly decreased testosterone level, count, viability and motility of sperm cells and testis weight and increased nitric oxide compared to the saline group (P=0.000). Administration of resveratrol and resveratrol plus morphine significantly increased motility, count and viability of sperm cells, somniferous tubule diameter and testosterone, while it decreased nitric oxide level compared to morphine group (P=0.025). It seems that resveratrol administration could increase the quality of spermatozoa and prevented morphine-induced adverse effects on sperm parameters.

RESUMEN: La morfina es uno de los alcaloides fenantreno del opio que induce efectos adversos en el sistema reproductivo masculino. El resveratrol es un fitoestrógeno y antioxidante de la uva roja. El objetivo principal de este trabajo fue investigar si el resveratrol puede inhibir los efectos adversos de la morfina sobre la viabilidad celular de los espermatozoides, el recuento y la motilidad, así como la histología de los testículos, la hormona testosterona y los niveles de óxido nítrico en ratones. Se dividieron, aleatoriamente, 48 ratas machos en 8 grupos (n = 6) y se trataron de forma intraperitoneal durante 14 días con solución salina normal, resveratrol (2, 8, 20 mg / kg / día), morfina (20 mg / kg / día ) y morfina (20 mg / kg / día) + resveratrol (2, 8, 20 mg / kg / día). Al final de los experimentos, se analizaron los parámetros espermáticos (viabilidad celular, recuento, motilidad y morfología), el peso de los testículos, el diámetro de los túbulos seminíferos, el nivel de la hormona testosterona y el óxido nítrico. Los datos fueron analizados con el software de SPSS para Windows (versión 20) usando una prueba de ANOVA de una vía seguida de la prueba post hoc de Tukey, y P <0,05 se consideró significativo. Los resultados indicaron que la administración de morfina disminuyó significativamente el nivel de testosterona, el recuento, la viabilidad y la motilidad de los espermatozoides y el peso de los testículos, además del aumento de óxido nítrico en comparación con el grupo salino (p = 0,000). La administración de resveratrol y resveratrol más morfina aumentó significativamente la motilidad, el recuento y la viabilidad de los espermatozoides, el diámetro de los túbulos seminíferos y la testosterona, mientras que disminuyó el nivel de óxido nítrico comparado con el grupo morfina (p = 0,025). En conclusión, la administración de resveratrol podría aumentar la calidad de los espermatozoides y prevenir los efectos adversos inducidos por la morfina sobre los parámetros espermáticos.
Descritores: Estilbenos/administração & dosagem
Genitália Masculina/efeitos dos fármacos
Morfina/toxicidade
-Motilidade Espermática
Espermatogênese/efeitos dos fármacos
Testículo/efeitos dos fármacos
Testosterona/sangue
Óxido Nítrico/sangue
Limites: Animais
Masculino
Ratos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-889068
Autor: Jiao, Yang; Wu, Yan; Du, Dong.
Título: Polydatin inhibits cell proliferation, invasion and migration, and induces cell apoptosis in hepatocellular carcinoma
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;51(4):e6867, 2018. graf.
Idioma: en.
Projeto: Innovative Research Program; . Science and Technology Program.
Resumo: Polydatin, a small molecule from Polygonum cuspidatum, has many biological functions, particularly anti-cancer effects. However, the anti-cancer effects of polydatin in hepatocellular carcinoma (HCC) have not been examined yet. In the present study, MTT assay, BrdU assay, transwell invasion assay, and wound healing assay were performed to determine cell proliferation, invasion and migration. Flow cytometry and TUNEL assay were used to measure cell apoptosis. Quantitative real-time PCR and western blotting assays were used to determine mRNA and protein expression levels. Xenograft experiment was performed to determine the in vivo anti-tumor effect of polydatin. Immunostaining was performed to analyze the expression of caspase-3 and Ki-67. Our results showed that polydatin inhibited cell proliferation in a concentration-dependent and time-dependent manner in the HCC cell lines. Polydatin also induced cell apoptosis in a concentration-dependent manner possibly via increasing the caspase-3 activity, and up-regulating the protein expression of caspase-3, caspase-9, Bax, and down-regulating the protein expression of Bcl-2. In addition, polydatin treatment had an inhibitory effect on cell proliferation, invasion and migration in HCC cell lines. Polydatin treatment also suppressed the Wnt/beta-catenin signaling activities in HCC cells. Polydatin treatment significantly reduced tumor growth in nude mice inoculated with HepG2 cells, suppressed the expression of Ki-67, and increased caspase-3 expression and TUNEL activity. Our data indicated the important role of polydatin for the suppression of HCC progression.
Descritores: Estilbenos/farmacologia
Movimento Celular/efeitos dos fármacos
Apoptose/efeitos dos fármacos
Carcinoma Hepatocelular/tratamento farmacológico
Proliferação de Células/efeitos dos fármacos
Glucosídeos/farmacologia
Neoplasias Hepáticas Experimentais/tratamento farmacológico
-Medicamentos de Ervas Chinesas
Western Blotting
Carcinoma Hepatocelular/patologia
Linhagem Celular Tumoral
Reação em Cadeia da Polimerase em Tempo Real
Citometria de Fluxo
Neoplasias Hepáticas Experimentais/patologia
Camundongos Endogâmicos BALB C
Camundongos Nus
Invasividade Neoplásica
Limites: Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME


  6 / 25 LILACS  
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Id: biblio-954126
Autor: Hasheminia, Dariush; Razavi, Sayed Mohammad; Nazari, Hamed; Khazaei, Saber; Soleimanzadeh, Pardis; Nazari, Hesamedin.
Título: Systemic supplement with resveratrol increased bone formation in rats' alveolar socket / Aumento de la formación ósea en el hueso alveolar de rata con suplemento sistémico con resveratrol
Fonte: Int. j. morphol;36(2):391-394, jun. 2018. tab, graf.
Idioma: en.
Resumo: Resveratrol in cell culture media increases osteoblastic markers. Also results from previous studies provide evidence for resveratrol positive effects on bone healing and bone production. In this preclinical study we investigated bone healing in rats by resveratrol systemic application. 30 Wistar male rats were divided into two groups (study group and control group). At first, maxillary second molars of rats were extracted. The rats were kept in laboratory for next 28 days. Study group received resveratrol 20 mg/kg by abdominal injection every day. The control group received placebo in the same manner that study group. Rats were sacrificed after 28 days and bone samples were collected from center of maxillary second molar socket. Samples were evaluated histologically for new bone formation, inflammation, necrosis, fibrosis and foreign body reaction. The mean difference of new bone formation in control group (28.30 %) and study group (45 %) were statistically significant (P=0.014). There were no significant differences in inflammation, fibrosis, necrosis and foreign body reaction (P>0.05). Resveratrol has positive effects on bone healing but more evidence needed from more clinical and animal studies.

El resveratrol en los medios de cultivo celular aumenta los marcadores osteoblásticos. Los resultados de estudios anteriores proporcionan evidencia de efectos positivos del resveratrol sobre la curación ósea y la producción ósea. En este estudio preclínico, investigamos la curación ósea en ratas mediante la aplicación sistémica de resveratrol. Se dividieron 30 ratas macho Wistar en dos grupos (estudio y control). Inicialmente se extrajeron los segundos molares maxilares de las ratas y los animales se mantuvieron en el laboratorio durante los siguientes 28 días. El grupo de estudio recibió todos los días resveratrol 20 mg/kg por inyección abdominal . El grupo control recibió placebo de la misma manera que el grupo estudio. Las ratas fueron sacrificadas después de 28 días y se recogieron muestras de hueso del centro del segundo molar maxilar. Las muestras se evaluaron histológicamente para la formación de hueso nuevo, inflamación, necrosis, fibrosis y reacción de cuerpo extraño. La media de formación de hueso nuevo en el grupo control (28,30 %) y en el grupo estudio (45 %) fueron estadísticamente significativas (P=0,014). No hubo diferencias significativas en la inflamación, fibrosis, necrosis y reacción al cuerpo extraño (P>0,05). El resveratrol tiene efectos positivos sobre la curación de los huesos, pero aún es necesario realizar más pruebas de estudios clínicos, como también en animales.
Descritores: Osteoblastos/efeitos dos fármacos
Osteoclastos/efeitos dos fármacos
Estilbenos/farmacologia
Desenvolvimento Ósseo/efeitos dos fármacos
-Osteogênese/efeitos dos fármacos
Ratos Wistar
Suplementos Nutricionais
Limites: Animais
Ratos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-954158
Autor: Ahmed, Muhammad Rashid; Shaikh, Masood Ahmed; Baloch, Naseer Ahmed; Nazir, Shakila; Abrar, Hina; Ulhaq, Hafiz Syed Imran.
Título: Neuroprotective potential of polydatin against motor abnormalities and dopaminergic neuronal loss in rotenone induced Parkinson model / Potencial neuroprotector de la polidatina contra las anomalías motoras y la pérdida neuronal dopaminérgica en un modelo de Parkinson inducido por la rotenona
Fonte: Int. j. morphol;36(2):584-591, jun. 2018. tab, graf.
Idioma: en.
Resumo: Among the neurodegenerative disorders, Parkinson disease (PD) is ranked as second most common. The pathological hallmark is selective degeneration of the dopaminergic neurons in the nigro-striatal regions of brain with appearance of the Lewy bodies. Present study explores the neuro-protective potential of polydatin in terms of amelioration of degeneration of dopaminergic neurons in nigro-striatal regions of brain and distorted neuromotor behavior in the rotenone model of Parkinson's disease. Thirty-six male Sprague Dawley rats were divided into three groups. Group A (control), Group B (rotenone treated) and Group C (rotenone+polydatin treated). Rotenone was administrated intraperitoneally (i.p) at a dose of 3 mg/kg/body weight while polydatin was given i.p. at a dose of 50 mg/ kg/body weight for four weeks. Then, animals were sacrificed; substantia nigra (SN) & striatum isolated from brain and five micron thick sections were prepared. Cresyl violet (CV), H&E and Immuno-histochemical staining using anti-TH antibody was done. Motor behavior was assessed weekly throughout the experiment using five different methods. Rotenone treated parkinsonian animals showed deterioration of motor behavior, weight loss, loss of dopaminergic neurons and diminished immune-reactivity in the sections from the nigrostriatal regions of these animals Polydatin+rotenone treatment showed contradicting effects to parkinsonism, with amelioration in weight loss, neuro-motor behavior, dopaminergic loss and immune-reactivity against dopaminergic neurons. Present study revealed a neuro-protective potential of polydatin in animal model of PD by ameliorating the neuro-motor abnormalities and degeneration of dopaminergic neurons in nigrostriatal regions.

Entre los trastornos neurodegenerativos, la enfermedad de Parkinson (EP) se clasifica como la segunda más común. El sello patológico es la degeneración selectiva de las neuronas dopaminérgicas en las regiones nigro-estriatales del cerebro, con la aparición de los cuerpos de Lewy. El presente estudio explora el potencial de protección neuronal de la polidatina en términos de la mejora de la degeneración de las neuronas dopaminérgicas en las regiones nigro-estriatales del cerebro y el comportamiento neuromotor distorsionado en el modelo de rotenona de la enfermedad de Parkinson. Treinta y seis ratas macho Sprague Dawley se dividieron en tres grupos: Grupo A (control), Grupo B (tratado con rotenona) y Grupo C (tratamiento con rotenona + polidatina). La rotenona se administró por vía intraperitoneal (i.p.) a una dosis de 3 mg/kg/peso corporal, mientras que la polidatina se administró i.p. a una dosis de 50 mg/kg/ peso corporal durante cuatro semanas. Posteriormente, los animales fueron sacrificados. Se aislaron la substantia nigra (SN) y cuerpo estriado de los cerebros y se realizaron secciones de cinco micras de espesor. Se realizó una tinción de violeta de cresilo (CV), H&E y tinción inmunohistoquímica usando anticuerpo anti-TH. El comportamiento motriz se evaluó semanalmente durante todo el experimento utilizando cinco métodos diferentes. Los animales parkinsonianos tratados con rotenona mostraron deterioro del comportamiento motriz, pérdida de peso, pérdida de neuronas dopaminérgicas y disminución de la reactividad inmune en las secciones de las regiones nigroestriadas. El tratamiento con polidatina + rotenona mostró efectos contrarios al parkinsonismo, con mejoría en la pérdida de peso, en el comportamiento motor, en la pérdida dopaminérgica y en la reactividad inmune contra las neuronas dopaminérgicas. El presente estudio reveló un potencial de protección neuronal de la polidatina en el modelo animal de la EP al mejorar las anomalías neuro-motoras y la degeneración de las neuronas dopaminérgicas en las regiones nigroestriatales.
Descritores: Doença de Parkinson/tratamento farmacológico
Estilbenos/administração & dosagem
Glucosídeos/administração & dosagem
-Doença de Parkinson/patologia
Rotenona/toxicidade
Imuno-Histoquímica
Dopamina
Ratos Sprague-Dawley
Fármacos Neuroprotetores
Modelos Animais de Doenças
Transtornos dos Movimentos/prevenção & controle
Degeneração Neural/prevenção & controle
Limites: Animais
Masculino
Ratos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-859826
Autor: Jesus, Luciano Henrique; Cavagni, Juliano; Carrard, Vinícius Coelho; Rösing, Cassiano Kuchenbecker; Sant'Ana Filho, Manoel.
Título: Effect of different presentations of resveratrol on cell proliferation and epitelial thickness of the oral mucosa of wistar rats
Fonte: Clin. biomed. res;37(3):175-180, 2017. ilus, graf.
Idioma: en.
Resumo: Introduction: Grape is one of the most important fruit crops across the world and can be consumed in different ways. There has been a growing interest in the role of antioxidants such as resveratrol, which can be found in grape skin, in oral and dental tissues. Thus, the objective of this study was to analyze the effect of different presentations of resveratrol on cell proliferation and epithelial thickness of the oral mucosa of Wistar rats. Methods: Fifty male Wistar rats were randomly divided into five groups: water/control, red wine, grape juice, 12% alcoholic solution/ethanol and aqueous solution of resveratrol. Samples of palatal and tongue mucosa were collected for a histomorphometric analysis using hematoxylin-eosin staining and the argyrophilic nucleolar organizer region (AgNOR) technique for quantification of cell proliferation. Results: As to epithelial thickness, both the tongue and the palate showed a statistically significant difference between the control group and the other groups, with greater decrease in the resveratrol and the wine groups. In the suprabasal layer of both the tongue and the palate epithelium, red wine reduced the rate of cell proliferation, while ethanol increased it. In the basal layer of the tongue epithelium, there was a statistically significant difference between the control, the grape juice and the resveratrol groups and the ethanol group, with increased cell proliferation in the ethanol group. Conclusions: Wine does not interfere in the physiological renewal of the basal layer of the buccal epithelium and exerts a protective action by reducing the cell proliferation rate of the suprabasal layer (AU)
Descritores: Proliferação de Células/efeitos dos fármacos
Epitélio/anatomia & histologia
Mucosa Bucal/citologia
Estilbenos/farmacologia
-Células Epiteliais/citologia
Etanol/química
Sucos de Frutas e Vegetais/análise
Ratos Wistar/anatomia & histologia
Vitis/química
Vinho/análise
Limites: Animais
Ratos
Responsável: BR18.1 - Biblioteca FAMED/HCPA


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Id: lil-779330
Autor: García-Díaz, Diego; Reyes-Farías, Marjorie; Ovalle-Marín, Angélica.
Título: Compuestos bioactivos e inflamaciónligada a obesidad / Bioactive compounds and inflammation linked to obesity
Fonte: Rev. chil. endocrinol. diabetes;7(1):21-24, ene.2014. ilus.
Idioma: es.
Resumo: Obesity has emerged among the major worldwide health threats. This pathology is characterized by the presence of a chronic inflammatory state in the overgrowing adipose tissue. This state has been related with an increased monocyte infiltration, and consequently with an establishment of an adipocyte-macrophage interaction, which in turn has been linked with the onset of obesity-related insulin resistance. Consequently, reducing this pathogenic crosstalk could comprise an interesting approach to counteract this inflammation. In this context, the screening of natural compounds with known anti-inflammatory/antioxidant properties over this crosstalk could be of highly significance. Popular culture and some investigations have point out that foods richs in polyphenols and essential fatty acids are known to possess these characteristics. It has been described that isolated bioactive compounds presents promising beneficial properties against the expression or secretion of inflammatory markers that are induced by the adipocyte-macrophage communication. Therefore, the proper evaluation of these compounds or the identification of new ones with potential characteristics is actually needed in aiming to reduce the increasing tendency of obesity-related pathologies, such as type 2 diabetes...
Descritores: Curcumina/administração & dosagem
Alimento Funcional
Inflamação/etiologia
Inflamação/prevenção & controle
Obesidade/complicações
-Tecido Adiposo
Estilbenos/administração & dosagem
Polifenóis/administração & dosagem
Quercetina/administração & dosagem
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


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Id: lil-762590
Autor: Noriega-González, J. E; Chirino, Y. I; Mata-Miranda, M. M; Vázquez-Zapién, G. J; Sánchez-Monroy, V.
Título: Effect of resveratrol on mitochondrial activity in differentiated mature adipocytes / Efecto del resveratrol sobre la actividad mitocondrial en adipocitos maduros diferenciados
Fonte: Int. j. morphol;33(3):1085-1092, Sept. 2015. ilus.
Idioma: en.
Resumo: Obesity has reached epidemic proportions, the World Health Organization (WHO) estimates that there are more than 1,000 million overweight adults world-wide. Furthermore, obesity is characterized as an overgrowth of white adipose tissue as a result of adipocyte hypertrophy and hyperplasia. Mitochondria is considered the source of energy within the adipocyte, since it contains the molecular machinery, and it is involved in a large number of metabolic pathways, besides the transformation of chemical energy into adenosine triphosphate. Mitochondria shortage and adipocyte dysfunction result in an excessive accumulation of triacylglycerol in the cytoplasm, which determines an imbalance between energy production and energy expenditure. Resveratrol (RSV) is a polyphenol found in different plants and its effects have been associated with mitochondrial biogenesis. An adipogenesis in vitro model (3T3-L1 preadipocytes) was used, and these cells were differentiated into mature adipocytes. Subsequently the effect of RSV on the adipocytes morphology, the lipid content and mitochondrial activity was evaluated using microscopic and flow cytometry techniques. The effect of RSV on differentiated mature adipocytes, was characterized by the decrease in lipid content and the consequently declination of the mitochondrial activity. 3T3-L1 preadipocytes retained the differentiation ability until passage 18. The RSV at doses of 25 and 50 µM for 48 hours in differentiated mature adipocytes promoted the decreased in lipid content probably due to an increase in mitochondrial activity in the early hours of RSV exposure, causing the consequently declination of mitochondrial activity at the end of 48 hours.

La obesidad ha tomado dimensiones epidémicas globales y la Organización Mundial de la Salud estima que hay más de 1,000 millones de adultos con sobrepeso. Así mismo, la obesidad se ha caracterizado como la expansión del tejido adiposo blanco condicionada por la hipertrofia e/o hiperplasia de los adipocitos. La mitocondria es considerada la fuente de energía dentro del adipocito, debido a que contiene la maquinaria molecular que dirige, a través de diversas vías metabólicas, la transformación de la energía química en adenosíntrifosfato. La escasez de mitocondrias así como su disfunción en el adipocito, resulta en una acumulación excesiva de triacilgliceroles en el citoplasma, lo que condiciona un desequilibrio entre producción de energía y gasto energético. El resveratrol (RSV) es un polifenol que se encuentra en diferentes grupos de plantas y sus efectos se han asociado con la inducción de genes para la biogénesis mitocondrial. Se empleó un modelo de adipogénesis (in vitro) materializado por una línea celular de preadipocitos 3T3-L1, mismos que se diferenciaron a adipocitos maduros. Posteriormente se evaluó el efecto del RSV sobre la morfología, contenido lipídico y actividad mitocondrial en los adipocitos maduros diferenciados a través de las técnicas: microscopía invertida, confocal y citometría de flujo. El efecto del RSV sobre los adipocitos maduros diferenciados, se caracterizó por la disminución del contenido lipídico y consecuentemente de la actividad mitocondrial. Los preadipocitos 3T3-L1 conservaron la capacidad de diferenciación hasta el pase 18. Por otra parte, el resveratrol a dosis de 25 y 50 µM durante 48 horas en adipocitos maduros diferenciados, promueve una disminución en el contenido lipídico probablemente debido a un aumento de la actividad mitocondrial en las primeras horas de exposición al tratamiento, provocando la disminución de la actividad mitocondrial al término de 48 horas.
Descritores: Adipócitos/efeitos dos fármacos
Estilbenos/farmacologia
-Células 3T3-L1
Células Cultivadas
Citometria de Fluxo
Mitocôndrias
Limites: Animais
Camundongos
Responsável: CL1.1 - Biblioteca Central



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