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ALVES, Sydney Hartz
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Id: lil-761166
Autor: OLIVEIRA, Daniele Carvalho; LORETO, Érico Silva de; MARIO, Débora Alves Nunes; LOPES, Paulo G. Markus; NEVES, Louise Vignolles; ROCHA, Marta Pires da; SANTURIO, Janio Morais; ALVES, Sydney Hartz.
Título: Sporothrix schenckiicomplex: susceptibilities to combined antifungal agents and characterization of enzymatic profiles / Complexo Sporothrix schenckii: susceptibilidade à combinação de antifúngicos e caracterização dos perfis enzimáticos
Fonte: Rev. Inst. Med. Trop. Säo Paulo;57(4):289-294, July-Aug. 2015. tab.
Idioma: en.
Projeto: CNPq.
Resumo: SUMMARYSporothrix schenckiiwas reclassified as a complex encompassing six cryptic species, which calls for the reassessment of clinical and epidemiological data of these new species. We evaluated the susceptibility of Sporothrix albicans(n = 1) , S. brasiliensis(n = 6) , S. globosa(n = 1), S. mexicana(n = 1) and S. schenckii(n = 36) to terbinafine (TRB) alone and in combination with itraconazole (ITZ), ketoconazole (KTZ), and voriconazole (VRZ) by a checkerboard microdilution method and determined the enzymatic profile of these species with the API-ZYM kit. Most interactions were additive (27.5%, 32.5% and 5%) or indifferent (70%, 50% and 52.5%) for TRB+KTZ, TRB+ITZ and TRB+VRZ, respectively. Antagonisms were observed in 42.5% of isolates for the TRB+VRZ combination. Based on enzymatic profiling, the Sporothrix schenckiistrains were categorized into 14 biotypes. Leucine arylamidase (LA) activity was observed only for S. albicansand S. mexicana. The species S. globosaand S. mexicanawere the only species without β-glucosidase (GS) activity. Our results may contribute to a better understanding of virulence and resistance among species of the genus Sporothrixin further studies.

RESUMOAvaliou-se a susceptibilidade de Sporothrix albicans(n = 1), S. brasiliensis(n = 1), S. globosa(n = 1), S. mexicana(n = 1) e S. schenckii(n = 36) frente à terbinafina (TRB) e a TRB em combinação com itraconazol (ITZ), cetoconazol (KTZ) e voriconazol (VRZ) pelo método de microdiluição ( checkerboard); o perfil enzimático destas espécies foi também avaliado, com base no kit API-ZYM. A maioria das interações foram aditivas (27,5%, 32,5% e 5%) ou indiferentes (70%, 50% e 52,5%) para TRB+KTZ, TRB+ITZ e TRB+VRZ, respectivamente. Antagonismo foi observado em 42,5% dos isolados para a combinação TRB+VRZ. Com base nos perfis enzimáticos, as cepas de Sporothrix schenckiievidenciaram 14 biotipos distintos. A atividade da leucina arilamidase (LA) só foi observada em S. albicanse S. mexicana.As espécies S. globosae S. mexicanaforam as únicas que não evidenciaram atividade da enzima β-glucosidase (GS). Estes resultados poderão contribuir para um melhor entendimento da virulência e resistência entre as espécies do gênero Sporothrixem futuros estudos.
Descritores: Antifúngicos/farmacologia
Sporothrix/efeitos dos fármacos
Sporothrix/enzimologia
-Itraconazol/farmacologia
Cetoconazol/farmacologia
Testes de Sensibilidade Microbiana
Naftalenos/farmacologia
Filogenia
Voriconazol/farmacologia
Limites: Humanos
Animais
Gatos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-1058003
Autor: Machado, Marília Alves; Campos, Diefrey Ribeiro; Lopes, Natália Lôres; Bastos, Isabela Pessôa Barbieri; Alves, Mariana Silva Revoredo; Correia, Thais Ribeiro; Scott, Fabio Barbour; Fernandes, Julio Israel.
Título: Efficacy of afoxolaner in the flea control in experimentally infested cats / Eficácia do afoxolaner no controle de pulgas em gatos experimentalmente infestados
Fonte: Rev. bras. parasitol. vet;28(4):760-763, Oct.-Dec. 2019. tab.
Idioma: en.
Resumo: Abstract The aim of this study was to evaluate the efficacy of a single dose of oral afoxolaner in controlling fleas in cats. Fourteen cats were used. The cats were given identification numbers, housed individually, artificially infested with Ctenocephalides felis felis, and treated (or not) with afoxolaner. Were divided into a treatment group and a control group (n = 7/group), on the basis of the fleas count hours after an infestation applied on Day (one-by-one allocation after ordering by count). At the start of the experimental protocol (designated day 0), the treated group received afoxolaner in a single dose of 2.5 mg/kg and the control group animals received a placebo. All animals were infested with 100 C. felis felis fleas two days before day 0, as well as on days 5, 12, 19, 26, 33, 40, 47, 54, and 63, parasite loads being evaluated at 48 h after each infestation. The efficacy of afoxolaner was 100% on day 2 and remained above 98% until day 42, decreasing to 95.3% by day 63. The findings confirm that a single dose of oral afoxolaner was effective in controlling C. felis felis in cats, and there were no observed adverse events.

Resumo O objetivo do estudo foi avaliar a eficácia de uma dose única de afoxolaner oral no controle de pulgas em gatos. Foram utilizados 14 gatos. Os animais foram identificados, alojados individualmente, infestados artificialmente com C. felis felis e tratados (ou não) com afoxolaner. Foram divididos em um grupo de tratamento e um grupo controle (n = 7/ grupo), com base na contagem de pulgas, horas após a infestação aplicada no dia (alocação de um por um após o período por contagem). No início do protocolo experimental (dia 0), o grupo tratado recebeu afoxolaner em dose inicial de 2,5 mg / kg e os animais do grupo controle receberam um placebo. Todos os animais foram infestados com 100 pulgas C. felis felis dois dias antes do dia 0, assim como nos dias 5, 12, 19, 26, 33, 40, 47, 54 e 63, sendo avaliadas as cargas parasitárias às 48 h após cada infestação. A eficácia do afoxolaner foi de 100% no dia 2 e permaneceu acima de 98% até o dia 42, diminuindo para 95,3% no dia 63. Os resultados confirmam que uma dose única de afoxolaner oral foi eficaz no controle de C. felis felis em gatos, e não houve eventos adversos observados.
Descritores: Doenças do Gato/parasitologia
Infestações por Pulgas/veterinária
Isoxazóis/administração & dosagem
Naftalenos/administração & dosagem
Antiparasitários/administração & dosagem
-Doenças do Gato/tratamento farmacológico
Estudos de Casos e Controles
Resultado do Tratamento
Infestações por Pulgas/tratamento farmacológico
Carga Parasitária
Sifonápteros
Limites: Animais
Masculino
Feminino
Gatos
Responsável: BR1.1 - BIREME


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Spósito, Andrei C
Caramelli, Bruno
Afiune Neto, Abrahao
Lima, José Jayme Galväo de
Bodanese, Luiz Carlos
Maranhäo, Raul C
Martinez, Tania Leme Rocha
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Id: lil-451704
Autor: Sposito, Andrei C; Caramelli, Bruno; Fonseca, Francisco A. H; Bertolami, Marcelo C; Afiune Neto, Abrahão; Souza, Aguinaldo David; Lottenberg, Ana Maria Pitta; Chacra, Ana Paula; Faludi, André A; Loures-Vale, Andréia A; Carvalho, Antônio Carlos; Duncan, Bruce; Gelonese, Bruno; Polanczyk, Carisi; Rodrigues Sobrinho, Carlos Roberto M; Scherr, Carlos; Karla, Cynthia; Armaganijan, Dikran; Moriguchi, Emílio; Saraiva, Francisco; Pichetti, Geraldo; Xavier, Hermes Toros; Chaves, Hilton; Borges, Jairo Lins; Diament, Jayme; Guimarães, Jorge Ilha; Nicolau, José Carlos; Santos, José Ernesto dos; Lima, José Jayme Galvão de; Vieira, José Luiz; Novazzi, José Paulo; Faria Neto, José Rocha; Torres, Kerginaldo P; Pinto, Leonor de Almeida; Bricarello, Liliana; Bodanese, Luiz Carlos; Introcaso, Luiz; Malachias, Marcus Vinícius Bolívar; Izar, Maria Cristina; Magalhães, Maria Eliane C; Schmidt, Maria Inês; Scartezini, Mariléia; Nobre, Moacir; Foppa, Murilo; Forti, Neusa A; Berwanger, Otávio; Gebara, Otávio C. E; Coelho, Otávio Rizzi; Maranhão, Raul C; Santos Filho, Raul Dias dos; Costa, Rosana Perim; Barreto, Sandhi; Kaiser, Sérgio; Ihara, Silvia; Carvalho, Tales de; Martinez, Tania Leme Rocha; Relvas, Waldir Gabriel Miranda; Salgado, Wilson.
Título: IV Diretriz Brasileira sobre Dislipidemias e Prevenção da Aterosclerose: Departamento de Aterosclerose da Sociedade Brasileira de Cardiologia
Fonte: Arq. bras. cardiol;88(supl.1):2-19, abr. 2007. tab.
Idioma: pt.
Descritores: Doença da Artéria Coronariana/prevenção & controle
Hiperlipidemias/terapia
Metabolismo dos Lipídeos/fisiologia
-Distribuição por Idade
Hipolipemiantes/uso terapêutico
Colesterol/sangue
Ácido Clofíbrico/uso terapêutico
Doença da Artéria Coronariana/etiologia
Doença da Artéria Coronariana/fisiopatologia
Dieta
Hiperlipidemias/complicações
Hiperlipidemias/fisiopatologia
Metabolismo dos Lipídeos/efeitos dos fármacos
Síndrome Metabólica/complicações
Naftalenos/uso terapêutico
Fatores de Risco
Distribuição por Sexo
Fumar/efeitos adversos
Triglicerídeos/sangue
Limites: Adulto
Idoso
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Tipo de Publ: Guia de Prática Clínica
Responsável: BR1.1 - BIREME


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Id: biblio-837967
Autor: Zhuang, Kai Wen; Dai, Ya Ling; Ran, Yu Ping; Lama, Jebina; Fan, Yi Ming.
Título: Tinea faciei on the right eyebrow caused by Trichophyton interdigitale
Fonte: An. bras. dermatol;91(6):829-831, Nov.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract Tinea faciei is a relatively uncommon dermatophyte infection entailing atypical clinical symptoms, usually misdiagnosed and treated with corticosteroids. The authors describe a case of tinea faciei on the right eyebrow caused by Trichophyton interdigitale. The patient was an 18-year-old girl, who had an inflammatory plaque with a scaly, pustular surface on the right eyebrow and upper eyelid, which had persisted for over 1 month. She was once misdiagnosed as having eczema and was treated using corticosteroid cream. A diagnosis of tinea faciei was made based on direct microscopy and culture. The sequencing of the nuclear ribosomal ITS region and β-tubulin gene of the isolate established its T. interdigitale lineage. The patient was cured by treatment with systemic terbinafine in combination with topical application of 1% naftifine-0.25% ketaconazole cream for 2 weeks.
Descritores: Tinha/patologia
Trichophyton/isolamento & purificação
Sobrancelhas/microbiologia
Sobrancelhas/patologia
Dermatoses Faciais/microbiologia
Dermatoses Faciais/patologia
-Tinha/tratamento farmacológico
Urease/análise
Microscopia Eletrônica de Varredura
Resultado do Tratamento
Dermoscopia
Dermatoses Faciais/tratamento farmacológico
Antifúngicos/uso terapêutico
Naftalenos/uso terapêutico
Limites: Humanos
Feminino
Adolescente
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: lil-788953
Autor: Nwinyi, Obinna C; Ajayi, Oluseyi O; Amund, Olukayode O.
Título: Degradation of polynuclear aromatic hydrocarbons by two strains of Pseudomonas
Fonte: Braz. j. microbiol;47(3):551-562, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT The goal of this investigation was to isolate competent polynuclear aromatic hydrocarbons degraders that can utilize polynuclear aromatic hydrocarbons of former industrial sites at McDoel Switchyard in Bloomington, Indiana. Using conventional enrichment method based on soil slurry, we isolated, screened and purified two bacterial species strains PB1 and PB2. Applying the ribotyping technique using the 16S rRNA gene analysis, the strains were assigned to the genus Pseudomonas (Pseudomonas plecoglossicida strain PB1 and Pseudomonas sp. PB2). Both isolates showed promising metabolic capacity on pyrene sprayed MS agar plates during the preliminary investigations. Using time course studies in the liquid cultures at calculated concentrations 123, 64, 97 and 94 ppm for naphthalene, chrysene, fluroanthene and pyrene, P. plecoglossicida strain PB1 and Pseudomonas sp. PB2 showed partial utilization of the polynuclear aromatic hydrocarbons. Naphthalene was degraded between 26% and 40%, chrysene 14% and 16%, fluroanthene 5% and 7%; pyrene 8% and 13% by P. plecoglossicida strain PB1 and Pseudomonas sp. PB2 respectively. Based on their growth profile, we developed a model R2 = 1 to predict the degradation rate of slow polynuclear aromatic hydrocarbon-degraders where all the necessary parameters are constant. From this investigation, we confirm that the former industrial site soil microbial communities may be explored for the biorestoration of the industrial site.
Descritores: Hidrocarbonetos Policíclicos Aromáticos/metabolismo
Pseudomonas/metabolismo
-Filogenia
Pseudomonas/classificação
Pseudomonas/genética
Pirenos/metabolismo
Solo/química
Microbiologia do Solo
Biodegradação Ambiental
Carbono/química
RNA Ribossômico 16S/genética
Crisenos/metabolismo
Naftalenos/metabolismo
Nitrogênio/química
Responsável: BR1.1 - BIREME


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Id: lil-762053
Autor: SHIKANAI-YASUDA, Maria Aparecida.
Título: Paracoccidioidomycosis treatment / Tratamento da paracoccidioidomicose
Fonte: Rev. Inst. Med. Trop. Säo Paulo;57(supl.19):31-37, Sept. 2015.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: SUMMARYConsidered to be an emerging endemic mycosis in Latin America, paracoccidioidomycosis is characterized by a chronic course and involvement of multiple organs in immunocompromised hosts. Infection sequelae are mainly related to pulmonary and adrenal insufficiency. The host-parasite interaction results in different expressions of the immune response depending on parasite pathogenicity, fungal load and genetic characteristics of the host. A few controlled and case series reports have shown that azoles and fast-acting sulfa derivatives are useful treatment alternatives in milder forms of the disease. For moderate/severe cases, more prolonged treatments or even parenteral routes are required especially when there is involvement of the digestive tract mucosa, resulting in poor drug absorption. Although comparative studies have reported that shorter treatment regimens with itraconazole are able to induce cure in chronically-infected patients, there are still treatment challenges such as the need for more controlled studies involving acute cases, the search for new drugs and combinations, and the search for compounds capable of modulating the immune response in severe cases as well as the paradoxical reactions.

RESUMOConsiderada micose endêmica emergente na América Latina, a paracoccidioidomicose é caracterizada por uma evolução crônica e envolvimento de múltiplos órgãos em pacientes com comprometimento imunológico. Sequelas da infecção estão relacionadas principalmente à insuficiência pulmonar e adrenal. A interação hospedeiro-parasito resulta em diferentes expressões da resposta imune dependendo da patogenicidade do parasito, carga fúngica e características genéticas do hospedeiro. Alguns estudos controlados e séries de casos têm demonstrado que azóis de ação rápida e derivados de sulfa constituem alternativas terapêuticas úteis nas formas mais leves da doença. Para casos moderados/graves, tratamentos mais prolongados ou mesmo por via parenteral são necessários especialmente quando há envolvimento de mucosa do trato digestivo, resultando em absorção deficiente de drogas. Embora estudos comparativos tenham relatado que esquemas terapêuticos mais curtos com itraconazol sejam capazes de induzir cura em pacientes cronicamente infectados, ainda existem desafios no tratamento, tais como a necessidade de maior número de estudos controlados envolvendo casos agudos, busca por novas drogas e combinações, compostos capazes de modular a resposta imune nos casos graves, e reações paradoxais.
Descritores: Paracoccidioidomicose/tratamento farmacológico
Sulfonamidas/uso terapêutico
Azóis/uso terapêutico
Anfotericina B/uso terapêutico
Antifúngicos/uso terapêutico
Naftalenos/uso terapêutico
-Índice de Gravidade de Doença
Resistência a Medicamentos
Ensaios Clínicos Controlados Aleatórios como Assunto
Infecções Fúngicas do Sistema Nervoso Central/tratamento farmacológico
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: BR1.1 - BIREME


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Id: biblio-841795
Autor: Almeida-Paes, Rodrigo; Brito-Santos, Fábio; Figueiredo-Carvalho, Maria Helena Galdino; Machado, Ana Caroline Sá; Oliveira, Manoel Marques Evangelista; Pereira, Sandro Antonio; Gutierrez-Galhardo, Maria Clara; Zancopé-Oliveira, Rosely Maria.
Título: Minimal inhibitory concentration distributions and epidemiological cutoff values of five antifungal agents against Sporothrix brasiliensis
Fonte: Mem. Inst. Oswaldo Cruz;112(5):376-381, May 2017. tab, graf.
Idioma: en.
Projeto: FAPERJ; . CNPq; . CNPq; . PAPES; . PAPES.
Resumo: BACKGROUND Sporothrix brasiliensis is the most virulent sporotrichosis agent. This species usually responds to antifungal drugs, but therapeutic failure can occur in some patients. Antifungal susceptibility tests have been performed on this species, but no clinical breakpoints (CBPs) are available. In this situation, minimal inhibitory concentration (MIC) distributions and epidemiological cutoff values (ECVs) support the detection of identification of resistant strains. OBJECTIVES To study the MIC distributions of five antifungal drugs against S. brasiliensis and to propose tentative ECVs. METHODS MICs of amphotericin B (AMB), itraconazole (ITR), ketoconazole (KET), posaconazole (POS), and terbinafine (TRB) against 335 S. brasiliensis strains were determined by the Clinical and Laboratory Standards Institute broth microdilution method. FINDINGS The proposed ECV, in µg/mL, for AMB, ITR, KET, POS, and TRB were 4.0, 2.0, 1.0, 2.0, and 0.25, respectively. Percentages of wild-type strains in our population for the above antifungal drugs were 98.48, 95.22, 95.33, 100, and 97.67%, respectively. MAIN CONCLUSIONS These ECVs will be useful to detect strains with resistance, to define CBPs, and to elaborate specific therapeutic guidelines for S. brasiliensis. Rational use of antifungals is strongly recommended to avoid the emergence of resistant strains and ensure the therapeutic effectiveness of sporotrichosis.
Descritores: Sporothrix/efeitos dos fármacos
Triazóis/farmacologia
Anfotericina B/farmacologia
Itraconazol/farmacologia
Cetoconazol/farmacologia
Antifúngicos/farmacologia
Naftalenos/farmacologia
-Resistência a Medicamentos
Gatos
Anti-Infecciosos
Limites: Humanos
Animais
Gatos
Responsável: BR1.1 - BIREME


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Id: lil-742561
Autor: Gallardo C, Natalia; Valenzuela L, Omar; Ibáñez V, Sebastián.
Título: Neumomediastino y necrosis cutánea en asociación a dermatomiositis: presentación de un caso clínico y revisión de la literatura / Pneumomediastinum and cutaneous necrosis in dermatomyositis: Report of one case
Fonte: Rev. méd. Chile;143(1):120-123, ene. 2015. ilus.
Idioma: es.
Resumo: We report a 37 years old male with a dermatomyositis treated with oral cyclophosphamide. He was admitted to the hospital due to a zone of skin necrosis with purulent exudate, located in the second left toe. A complete blood count showed a leukocyte count of 2,600 cells/mm³. A Chest CAT scan showed a pneumomediastinum with emphysema of adjacent soft tissue. Cyclophosphamide was discontinued and leukocyte count improved. The affected toe was amputated and a chest CAT scan showed a partial resolution of the pneumomediastinum. We discuss and review the pathogenesis, clinical presentation and management of pneumomediastinum and cutaneous necrosis in association with dermatomyositis.
Descritores: Benzoxazinas/uso terapêutico
Canabinoides/agonistas
Encefalomielite Autoimune Experimental/tratamento farmacológico
Encefalomielite Autoimune Experimental/patologia
Morfolinas/uso terapêutico
Naftalenos/uso terapêutico
Neurônios/efeitos dos fármacos
Oligodendroglia/efeitos dos fármacos
-Análise de Variância
Precursor de Proteína beta-Amiloide/metabolismo
Caspase 9/metabolismo
/metabolismo
CASPASE ABATTOIRS/metabolismo
Contagem de Células/métodos
Sistema Nervoso Central/patologia
Citocinas/genética
Citocinas/metabolismo
Modelos Animais de Doenças
Encefalomielite Autoimune Experimental/complicações
Macrófagos/efeitos dos fármacos
Exame Neurológico
Degeneração Neural/etiologia
Degeneração Neural/prevenção & controle
Poli(ADP-Ribose) Polimerases/metabolismo
Medula Espinal/efeitos dos fármacos
Medula Espinal/patologia
Linfócitos T/efeitos dos fármacos
Fatores de Tempo
Limites: Animais
Feminino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: lil-715536
Autor: Azambuja, Christiane Venske de Almeida; Pimmel, Luciana Alves; Klafke, Gabriel Baracy; Xavier, Melissa Orzechowski.
Título: Onychomycosis: clinical, mycological and in vitro susceptibility testing of isolates of Trichophyton rubrum
Fonte: An. bras. dermatol;89(4):581-586, Jul-Aug/2014. tab.
Idioma: en.
Resumo: BACKGROUND: Onychomycosis or nail fungal infection is the most common nail disease. Despite the wide range of studies on this condition, it remains difficult to establish the correct diagnosis and effective treatment. OBJECTIVES: To evaluate the efficacy of classical laboratory methods for the diagnosis of onychomycosis, and the in vitro susceptibility of the its main etiological agent to antifungals used in routine. METHODS: Nail samples of 100 patients with clinically suspected feet onychomycosis were collected to confirm the diagnosis by direct mycological examination and fungal culture. In vitro antifungal susceptibility testing was performed against strains of the main dermatophyte isolated by microdilution, according to the standardized protocol (M38-A2 - CLSI) RESULTS: Clinical diagnosis of onychomycosis was confirmed by laboratory analysis in 59% of patients. Of these, 54.2% were positive only in direct mycological examination, 44.1% in direct mycological examination and culture, and one case (1.7%) was positive only in culture, resulting in weak agreement between these tests (Kappa = 0.385; p <0.001) High minimum inhibitory concentration values of fluconazole and itraconazole were observed in 66.7% and 25.0% of isolates of T. rubrum tested. Additionally, high MIC values of terbinafine and ciclopirox was detected in only one isolate, and this was one of the strains in which in vitro activity of itraconazole and fluconazole has not been proven. CONCLUSIONS: Poor agreement was observed between direct mycological examination and culture for the diagnosis of onychomycosis, with direct mycological examination being significantly more sensitive. Except for fluconazole, the other three antifungals tested showed good in vitro activity against clinical isolates of T. rubrum. .
Descritores: Antifúngicos/farmacologia
Onicomicose/diagnóstico
Onicomicose/microbiologia
Trichophyton/efeitos dos fármacos
Trichophyton/isolamento & purificação
-Estudos Transversais
Relação Dose-Resposta a Droga
Fluconazol/farmacologia
Itraconazol/farmacologia
Testes de Sensibilidade Microbiana
Naftalenos/farmacologia
Onicomicose/tratamento farmacológico
Estudos Prospectivos
Piridonas/farmacologia
Reprodutibilidade dos Testes
Limites: Adulto
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: lil-712952
Autor: Mishra, Nripendra Nath; Ali, Shakir; Shukla, Praveen K..
Título: Arachidonic acid affects biofilm formation and PGE2 level in Candida albicans and non-albicans species in presence of subinhibitory concentration of fluconazole and terbinafine
Fonte: Braz. j. infect. dis;18(3):287-293, May-June/2014. tab, graf.
Idioma: en.
Projeto: CSIR-CDRI Communication.
Resumo: Candida albicans utilizes arachidonic acid (AA) released during the course of infection (Candidiasis) from phospholipids of infected host cell membranes and synthesizes extracellular prostaglandin(s) which play an important role in hyphae formation and host cell damage. C. albicans biofilms secrete significantly more prostaglandin(s) and evidence suggests that Candida biofilms have dramatically reduced susceptibility to majority of antifungal drugs. AA influences the saturation level of lipids and fluidity of yeast cell membranes. Therefore the aim of this study was to evaluate the effect of AA alone or in combination with antifungal agents on biofilm formation and production of prostaglandin (PGE2) in C. albicans, C. parapsilosis, C. glabrata, C. tropicalis, and C. albicans amphotericin B resistant strain (AmBR). Maximum biofilm formation was found to be in the case of C. albicans compared to C. non-albicans species. However, among the non-albicans species C. tropicalis exhibited highest biofilm formation. Treatment with AA in combination with subinhibitory concentrations of fluconazole and terbinafine separately exhibited significant (p < 0.05) reduction in biofilm formation against C. glabrata, C. parapsilosis, C. tropicalis and AmBR as compared to their individual effect. Further, these two antifungal agents in combination with AA caused an increase in production of prostaglandin from fungal cell itself which was significant (p < 0.05) in case of all the strains tested.
Descritores: Antifúngicos/farmacologia
Ácido Araquidônico/farmacologia
Biofilmes/efeitos dos fármacos
Candida/efeitos dos fármacos
Dinoprostona/análise
Fluconazol/farmacologia
Naftalenos/farmacologia
-Biofilmes/crescimento & desenvolvimento
Candida albicans/efeitos dos fármacos
Candida/química
Candida/classificação
Testes de Sensibilidade Microbiana
Microscopia de Fluorescência
Responsável: BR1.1 - BIREME



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