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Id: lil-619729
Autor: Oré De La Cruz, Jhames Iván.
Título: Diagnóstico de amiloidosis mediante biopsia de glándula salival menor / Diagnosis of amiloidosis biopsy of minor glands
Fonte: Kiru;3(1):7-10, ene.-jun. 2006.
Idioma: es.
Resumo: Objetivo: diagnosticar amiloidosis biopsia de glándula salival menor. Material y método: se utilizó el método de observación, retrospectivo y descriptivo. El estudio consistió en obtener resultados de 60 pacientes que se realizaron biopsias de glándula salival menor para descartar amiloidosis. Los pacientes estaban hospitalizados por otras patologías y tenían sospecha clínica de amiloidosis; por esa razón todos los pacientes traían una hoja de interconsulta. Resultados: de las 60 biopsias, 38 resultaron negativas y 22 resultaron positivas para amiloidosis. De los 60 pacientes, 13 tuvieron antecedentes de TBC, de los cuales 7 hicieron la enfermedad de amiloidosis. Conclusiones: la biopsia de glándula salival es útil para diagnosticar amiloidosis; además, es más accesible en comparación con las biopsias de riñón, mucosa rectal y grasa abdominal, que también se realizan para diagnosticar amiloidosis. De 16 pacientes con biopsia que tenían síndrome nefrótico, todos resultaron negativos para amiloidosis.

Objective: to diagnose amyloidosis biopsy of minor salivary gland. Material and Method: I used the method of observation, retrospective and descriptive. The study consisted of results of 60 patients who were minor salivary gland biopsied to rule out amyloidosis. Patients were hospitalized for other ailments and had clinical suspicion of amyloidosis; that is why all patients brought a sheet interconsultation. Results: Of the 60 biopsies, 38 were negative and 22 were positive for amyloidosis. Of the 60 patients, 13 had a history of TB, of which 7 were disease amyloidosis. Conclusions: The biopsy of the salivary gland is useful for diagnosing amyloidosis; moreover, they are more accessible compared to the biopsies of Kidney, rectal mucosa and abdominal fat, which also are made for diagnosing amyloidosis. Biopsies of 16 patients who had nephrotic syndrome, all were negative for amyloidosis.
Descritores: Amiloidose/diagnóstico
Glândulas Salivares Menores/patologia
Vermelho Congo
-Epidemiologia Descritiva
Estudos Prospectivos
Estudos Observacionais como Assunto
Limites: Humanos
Masculino
Adulto
Idoso
Idoso de 80 Anos ou mais
Pessoa de Meia-Idade
Feminino
Responsável: PE264.3 - Biblioteca


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Id: lil-676890
Autor: Melo, Poliana de Castro; Ferreira, Luciano Menezes; Nader Filho, Antônio; Zafalon, Luiz Francisco; Vicente, Hinig Isa Godoy; Souza, Viviane de.
Título: Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis
Fonte: Braz. j. microbiol;44(1):119-124, 2013. tab.
Idioma: en.
Resumo: Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and the presence of the icaA and icaD genes. The results revealed that 85% of the isolates tested produced slime on the Congo red agar, 98.9% of the isolates produced biofilms in vitro by adhering to sterile 96-well "U" bottom polystyrene tissue culture plates, and 95.7% of the isolates carried the icaA and icaD genes. The results of the phenotypic tests for biofilm formation were compared with those of the molecular analysis, and the sensitivity and specificity of the Congo red agar test were 88.9% and 100%, respectively, while those of the microtitre plate test were 100% and 25%, respectively. When the phenotypic methods for the detection of biofilm producers, namely growth on microtitre plates and Congo red agar, were compared, the sensitivity and specificity were 86% and 100%, respectively. Therefore, growth on Congo red agar and the microtitre plate test are methods that could be used to determine whether an isolate has the potential for biofilm production.
Descritores: Biofilmes
Determinação/análise
Técnicas In Vitro
Mastite Bovina
Fenótipo
Reação em Cadeia da Polimerase/métodos
Infecções Estreptocócicas
Staphylococcus aureus/genética
Staphylococcus aureus/isolamento & purificação
Vermelho Congo/análise
-Bovinos
Genótipo
Métodos
Limites: Animais
Bovinos
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-649515
Autor: França, Chirles A; Peixoto, Rodolfo M; Cavalcante, Marielly B; Melo, Natoniel F; Oliveira, Celso José B; Veschi, Josir Laine A; Mota, Rinaldo A; Costa, Mateus M.
Título: Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil / Resistência antimicrobiana de Staphylococcus spp. de mastite de pequenos ruminantes no Brasil
Fonte: Pesqui. vet. bras = Braz. j. vet. res;32(8):747-753, ago. 2012. ilus, tab.
Idioma: en.
Projeto: Pernambuco State Science and Technology Support Foundation. First Project Program-PPP; . Sisal Regional Development Institute.
Resumo: The study aimed to determine the antimicrobial resistance patterns and to identify molecular resistance markers in Staphylococcus spp. (n=210) isolated from small ruminant mastitis in Brazil. The antimicrobial resistance patterns were evaluated by the disk diffusion test and by detection of the presence of mecA, blaZ, ermA, ermB, ermC and msrA genes by PCR. The efflux pump test was performed using ethidium bromide and biofilm production was determined by Congo red agar test along with PCR for detection of the icaD gene. The isolates were most resistant to amoxicillin (50.0%), streptomycin (42.8%), tetracycline (40.4%), lincomycin (39.0%) and erythromycin (33.8%). Pan-susceptibility to all tested drugs was observed in 71 (33.8%) isolates and 41 Staphylococcus isolates were positive for the efflux pump. Although phenotypic resistance to oxacillin was observed in 12.8% of the isolates, none harbored the mecA gene. However, 45.7% of the isolates harbored blaZ indicating that beta-lactamase production was the main mechanism associated with staphylococci resistance to beta-lactams in the present study. The other determinants of resistance to antimicrobial agents ermA, ermB, ermC, and msrA were observed in 1.4%, 10.4%, 16.2%, and 0.9% of the isolates, respectively. In addition, the icaD gen was detected in 32.9% of the isolates. Seventy three isolates (54 from goats and 19 from sheep) were negative for all resistance genes tested and 69 isolates presented two or more resistance genes. Association among blaZ, ermA, ermB, ermC and efflux pump were observed in 17 isolates, 14 of which originated from goats and three from sheep. The data obtained in this study show the resistance of the isolates to beta-lactamics, which may be associated with the use of antimicrobial drugs without veterinary control.

O presente trabalho teve como objetivo determinar os padrões de resistência a agentes antimicrobianos e identificar marcadores moleculares de resistência em Staphylococcus spp. (n=210) isolados de mastite de pequenos ruminantes no Brasil. Os padrões de resistência a agentes antimicrobianos foram avaliados pelo teste de difusão em disco e pela detecção da presença dos genes mecA, blaZ, ermA, ermB, ermC e msrA via PCR. O teste da bomba de efluxo foi realizado utilizando brometo de etídio e a produção de biofime foi determinada pelo teste do vermelho congo em paralelo com o PCR para detecção do gene icaD. Os isolados foram mais resistentes a amoxicilina (50,0%), estreptomicina (42,8%), tetraciclina (40,4%), lincomicina (39,0%) e eritromicina (33,8%). Setenta e um (33,8%) isolados foram sensíveis a todas as drogas testadas e 41 foram positivos para a bomba de efluxo. Embora a resistência fenotípica a oxacilina tenha sido observada observada em 12,8% dos isolados, nenhum possuiu o gene mecA. Entretanto, 45,7% dos isolados continham a gene blaZ, indicando que a produção de beta-lactamases foi o principal mecanismo associado com a resistência dos Staphylococcus aos beta-lactâmicos. Os outros determinantes de resistência a agentes antimicrobianos ermA, ermb, ermC e msrA foram observados em 1,4%, 10,4%, 16,2% e 0,9% dos isolados respectivamente. Além disso, o gene icaD foi detectado em 32,9% dos isolados. Setenta e três isolados (54 de cabras e 19 de ovelhas) foram negativos para todos os genes de resistência testados e 69 isolados apresentaram dois ou mais genes de resistência. A associação entre blaZ, ermA, ermB, ermC e bomba de efluxo foi observada em 17 isolados dos quais 14 eram oriundos de cabras e três de ovelhas. Os dados obtidos no presente estudo indicam a resistência dos isolados aos beta-lactâmicos, o que pode estar associado ao uso sem controle veterinário destas drogas nos animais.
Descritores: Mastite Bovina/imunologia
Resistência Microbiana a Medicamentos/genética
Staphylococcus
Staphylococcus/genética
Staphylococcus/isolamento & purificação
Testes de Sensibilidade Microbiana/veterinária
-Oxacilina/imunologia
Penicilinase/imunologia
Resistência a Meticilina/imunologia
Vermelho Congo
Limites: Animais
Bovinos
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-622459
Autor: Oliveira, Ederson Valei Lopes de; Pozetti, Ana Carolina Garcia; Pozetti, Eurides Maria de Oliveira; Antonio, João Roberto; Michalany, Nilceo Schwery.
Título: Primary systemic amyloidosis associated with multiple myeloma / Amiloidose sistêmica primária associada ao mieloma múltiplo
Fonte: An. bras. dermatol;87(1):119-122, Jan.-Feb. 2012. ilus.
Idioma: en.
Resumo: This case report is about a 48-year-old female patient with systemic amyloidosis and multiple myeloma simultaneously. Amyloid cutaneous infiltrative lesions like papules, nodules, or plaques with a serous-hemorrhagic aspect were found in the eyelids, neck and retroauricular region, among others. She had presented intermittent papular lesions on the upper eyelids one year before, which worsened following local trauma. A local skin biopsy showed amorphous and eosinophilic substance in the dermis. Congo red staining confirmed the amyloid deposits. Abnormal exams: proteinuria (570mg/24h), Bence-Jones proteinuria and clonal plasma cells (70%) found in myelogram. Following the diagnosis of multiple myeloma based on amyloid skin lesions, the patient was referred to the Hematology service and died 5 months after the diagnosis.

Relatamos um caso de uma paciente de 48 anos com amiloidose sistêmica associada a mieloma múltiplo. Lesões infiltrativas cutâneas como pápulas, nódulos ou placas com aspecto sero-hemorrágico podem ser localizados nas pálpebras, pescoço, região retroauricular dentre outras. No presente caso, as pálpebras foram acometidas por pápulas, há 1 ano, de caráter intermitente e piora após trauma local. Biópsia local evidenciou material amorfo e eosinofílico na derme. A coloração vermelho do Congo confirmou presença de substância amiloide. Exames anormais: proteinúria de 570mg/24 horas, proteinúria de Bence-Jones positiva e mielograma com 70% de plasmócitos atípicos. Assim, realizou-se o diagnóstico de mieloma múltiplo a partir de manifestações cutâneas de amiloidose. Paciente encaminhada ao serviço de hematologia e foi a óbito em 5 meses.
Descritores: Amiloidose/patologia
Pálpebras/patologia
Mieloma Múltiplo/patologia
Neoplasias Cutâneas/patologia
-Amiloidose/complicações
Vermelho Congo
Evolução Fatal
Mieloma Múltiplo/etiologia
Limites: Feminino
Humanos
Pessoa de Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: lil-614619
Autor: Bhattacharya, Sourav; Das, Arijit; G, Mangai; K, Vignesh; J, Sangeetha.
Título: Mycoremediation of Congo red dye by filamentous fungi
Fonte: Braz. j. microbiol;42(4):1526-1536, Oct.-Dec. 2011. graf.
Idioma: en.
Resumo: Azo, anthroquinone and triphenylmethane dyes are the major classes of synthetic colourants, which are difficult to degrade and have received considerable attention. Congo red, a diazo dye, is considered as a xenobiotic compound, and is recalcitrant to biodegradative processes. Nevertheless, during the last few years it has been demonstrated that several fungi, under certain environmental conditions, are able to transfer azo dyes to non toxic products using laccases. The aim of this work was to study the factors influencing mycoremediation of Congo red. Several basidiomycetes and deuteromycetes species were tested for the decolourisation of Congo red (0.05 g/l) in a semi synthetic broth at static and shaking conditions. Poor decolourisation was observed when the dye acted as the sole source of nitrogen, whereas semi synthetic broth supplemented with fertilizer resulted in better decolourisation. Decolourisation of Congo red was checked in the presence of salts of heavy metals such as mercuric chloride, lead acetate and zinc sulphate. Decolourisation parameters such as temperature, pH, and rpm were optimized and the decolourisation obtained at optimized conditions varied between 29.25- 97.28 percent at static condition and 82.1- 100 percent at shaking condition. Sodium dodecyl sulphate polyacrylamide gel electrophoretic analysis revealed bands with molecular weights ranging between 66.5 to 71 kDa, a characteristic of the fungal laccases. High efficiency decolourisation of Congo red makes these fungal forms a promising choice in biological treatment of waste water containing Congo red.
Descritores: Basidiomycota
Corantes Azur/análise
Lacase/análise
Vermelho Congo/análise
Xenobióticos/análise
-Biodegradação Ambiental
Microbiologia Ambiental
Métodos
Técnicas
Tipo de Publ: Relatório Técnico
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-614595
Autor: Samanta, A. K; Kolte, Atul P; Senani, S; Sridhar, Manpal; Jayapal, Natasha.
Título: A simple and efficient diffusion technique for assay of endo b-1, 4-xylanase activity
Fonte: Braz. j. microbiol;42(4):1349-1353, Oct.-Dec. 2011. ilus, tab.
Idioma: en.
Resumo: Endo-β-1, 4-xylanases is thought to be of great significance for several industries namely paper, pharmaceuticals, food, feed etc. in addition to better utilization of lignocellulosic biomass. The present investigation was aimed to develop an easy, simple and efficient assay technique for endo-β-1, 4-xylanases secreted by the aerobic fungi. Under the proposed protocol, 9 g/L xylan containing agar was prepared in 100 mM phosphate buffer at different pH (4.5, 5.5 and 6.5). The sterilized xylan agar was dispensed in 90 mm petri dishes. 100 µl of culture supernatant of 12 fungal isolates was added to the wells and left overnight at 31±10C. The petri dishes were observed for zone of clearance by naked eye and diameter was measured. Congo red solution (1 g/L) was applied over the petri dishes as per the established protocol and thereafter plates were flooded with 1M Sodium chloride solution for the appearance of zone of clearance. The diameter for zone of clearance by the proposed method and the established protocol was almost identical and ranged from 21 to 42 mm at different pH depending upon the activity of endo-β-1, 4-xylanases. Change of pH towards alkaline side enabled similar or marginal decrease of diameter for the zone of clearance in most of the fungal isolates. The specific activities of these fungal isolates varied from 1.85 to 11.47 IU/mg protein. The present investigation revealed that the proposed simple diffusion technique gave similar results as compared to the established Congo red assay for endo-β-1, 4-xylanases. Moreover, the present technique avoided the cumbersome steps of staining by Congo red and de-staining by sodium chloride.
Descritores: Biomassa
/análise
ENDO-1,ABBREVIATIONS AS TOPIC-BETA-XILANASES/análise
Vermelho Congo/análise
Xilanos/análise
-Microbiologia Industrial
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


  7 / 21 LILACS  
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Id: lil-604112
Autor: Melo, Bárbara Lima Araújo; Costa, Igor Santos; Goes, Clara de Assis Martins; Tigre, Celina Aguiar Frota; André, Nara Frota.
Título: Amiloidose macular de localização atípica / An unusual presentation of macular amyloidosis
Fonte: An. bras. dermatol;86(4,supl.1):24-27, jul,-ago. 2011. ilus.
Idioma: pt.
Resumo: A amiloidose macular é uma forma cutânea primária de amiloidose, caracterizada tipicamente por máculas acastanhadas, acometendo predominantemente a região interescapular. O presente caso é o de uma mulher de 45 anos, há um ano com manchas hipercrômicas, rendilhadas e não pruriginosas, em membros superiores, região cervical, sacral e membros inferiores. No estudo histopatológico da biópsia de pele, foi evidenciada substância amiloide amorfa no topo das papilas dérmicas, que se tornou mais evidente após a coloração pelo vermelho Congo. Não havia alterações sistêmicas associadas. Além da localização atípica e extensa da amiloidose cutânea macular, o presente caso procura acrescentar dados para esclarecimento do tema.

Macular amyloidosis is a form of cutaneous amyloidosis characterized by dusky-brown lesions usually located on the upper back between the shoulder blades. This report describes the case of a 45year-old female presenting with hyperpigmented macules and lace-like, non-pruritic erythema in the sacral and cervical region as well as on both arms and legs. Histology revealed amyloid deposits in the papillary dermis which exhibited apple-green birefringence after Congo red staining. There were no systemic findings. This is a case of macular amyloidosis with an unusual presentation. The literature on the subject is also reviewed.
Descritores: Amiloidose/patologia
Dermatopatias Metabólicas/patologia
-Corantes
Vermelho Congo
Limites: Feminino
Humanos
Pessoa de Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: lil-588113
Autor: Rocha, A J; Silva, C J; Leopoldino, J F S; Andrade, V P.
Título: Intracerebral amyloidoma: imaging findings might support preoperative diagnosis / Amiloidoma intracerebral: os achados de imagem podem presumir o diagnóstico pré-operatório
Fonte: Arq. neuropsiquiatr;69(2b):413-413, 2011. ilus.
Idioma: en.
Descritores: Amiloidose/diagnóstico
Encefalopatias/diagnóstico
-Corantes
Vermelho Congo
Tomografia Computadorizada por Raios X
Limites: Adulto
Feminino
Humanos
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: lil-526892
Autor: Pérez S., Soledad.
Título: Amiloidosis: enfermedad desafiante en su diagnóstico y tratamiento / Amyloidosis: diagnosis and treatment
Fonte: Rev. chil. reumatol;25(1):37-41, 2009. tab.
Idioma: es.
Resumo: La amiloidosis es una enfermedad sistémica caracterizada por el depósito de fibrillas amiloídeas en diversos órganos, lo que lleva a un deterioro y falla progresiva de éstos, afectando sustancialmente la sobrevida del paciente. Se analizaron los registros médicos de 34 pacientes egresados con diagnóstico de amiloidosis del Hospital Clínico de la Universidad de Chile en un período de 15 años. El promedio del tiempo entre la aparición de síntomas y el diagnóstico fue aproximadamente de seis meses. Los compromisos sistémicos de mayor relevancia fueron el renal y cardíaco, influyendo este último notablemente en la sobrevida. El laboratorio inmunológico tuvo un rol fundamental en identificar a los pacientes portadores de una paraproteína y de esta manera apoyar el diagnóstico. Es necesario plantearse este diagnóstico diferencial en todo paciente con compromiso sistémico de causa no clara y más aún cuando está asociado un trastorno mieloproliferativo.

Amyloidosis is a systemic disease characterized by the deposition of amyloid fibrils in many organs, leading to a progressive deterioration and failure of these, substantially affecting the survival of the patient. We present 34 medical records of patients that were hospitalized with the diagnosis of amyloidosis in the Hospital of the Universidad de Chile, over a period of fifteen years. The average time between the onset of symptoms and diagnosis was approximately six months. The most important systemic affectations were to the kidney and heart, the latter significantly influences survival. The immunology laboratory plays a fundamental role in identifying patients with paraprotein, thus supporting diagnosis. It is necessary to consider this differential diagnosis in all patients with systemic symptoms of no clear cause, especially when it is associated to a myeloproliferative disorder.
Descritores: Amiloidose/diagnóstico
-Amiloidose/mortalidade
Amiloidose/tratamento farmacológico
Evolução Clínica
Cardiopatias/diagnóstico
Chile/epidemiologia
Eletroforese/métodos
Nefropatias/diagnóstico
Estudos Retrospectivos
Vermelho Congo
Sinais e Sintomas
Taxa de Sobrevida
Fatores de Tempo
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso de 80 Anos ou mais
Responsável: CL1.1 - Biblioteca Central


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Id: lil-520229
Autor: Abdallah, Fethi Ben; Chaieb, Kamel; Zmantar, Tarek; Kallel, Hela; Bakhrouf, Amina.
Título: Adherence assays and Slime production of Vibrio alginolyticus and Vibrio parahaemolyticus / Ensaios de adesão e produção de muco por Vibrio alginolyticus e Vibrio parahaemolyticus
Fonte: Braz. j. microbiol;40(2):394-398, Apr.-June 2009. ilus, tab.
Idioma: en.
Resumo: In this study we investigated the phenotypic slime production of Vibrio alginolyticus and Vibrioparahaemolyticus strains, food-borne pathogens, using a Congo red agar plate assay. Furthermore, westudied their ability to adhere to abiotic surfaces and Vero cells line. Our results showed that only V.alginolyticus ATCC 17749 was a slime-producer developing almost black colonies on Congo red agar plate.Adherence to glace tube showed that all V. alginolyticus strains were more adherent than V. parahaemolyticus.Only V. alginolyticus ATCC 17749 was found to be able to form biofilm on polystyrene microplate wells (OD570= 0.532). Adherence to Vero cells showed that all tested strains were non adherent after 30 min, however after60 min all the studied strains become adherent. The percentage of adherence ranged from1.23% to 4.66%.

Neste estudo, investigou-se a produção de muco por cepas de Vibrio alginolyticus e Vibrio parahaemolyticus através do teste em placa de ágar com vermelho congo. Estudou-se também a capacidade de adesão à superfícies abióticas e células Vero. Os resultados indicaram que somente V. alginolyticus ATCC 17749 produziu muco, formando colônias quase negras nas placas de ágar com vermelho congo. O teste de adesão a tubos de vidro indicou que as cepas de V. alginolyticus foram maisaderentes do que as de V. parahaemolyticus. Somente V. alginolyticus ATCC 17749 foi capaz de formar biofilme nos poços das microplacas de poliestireno (OD570=0,532). Testes de adesão a células Vero mostraram que nenhuma das cepas apresentou adesão em 30 min, mas todas aderiram após 60 min. Aporcentagem de adesão variou de 1,23% a 4,66%.
Descritores: Adesões Focais
Aderência Bacteriana
Biofilmes/crescimento & desenvolvimento
Muco
Vermelho Congo/análise
Vibrio alginolyticus/isolamento & purificação
-Ágar
Métodos
Técnicas
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica



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