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Id: lil-549972
Autor: Mehrdad, Jahanshahi; Bahadoran, H; Asadi, M. H.
Título: Acute effects of sulphur mustard gas on the number of lymphocytes in the rat's spleen / Efectos agudos del gas mostaza sobre el número de linfocitos en el bazo de rata
Fonte: Int. j. morphol;26(2):433-436, jun. 2008. tab.
Idioma: en.
Resumo: Sulphur mustard (SM), commonly known as mustard gas is an alkylating agent that causes serious blisters upon contact with human skin. SM is frequently used as a chemical warfare agent. There is some evidence for sulfur mustard-induced lymph system effects in humans. Between 2000-2001, 42 male albino Wistar rats were used. After accommodation with environment, we divided rats to control, sham and experimental groups (2.5 mg/kg, 5 mg/kg, 10 mg/kg, 20 mg/kg and 40 mg/kg). Then we injected sulphur mustard oil in rat's intraperitoneal space. Then their spleens were removed for histological verification. Our results showed that significant difference in lymphocytes number in experimental groups after 24 hours. The number of lymphocytes in 5, 10, 20 and 40 mg/kg groups was increased and this increase in 40 mg/kg group was more than the other groups. We concluded that the number of lymphocytes increased due to exposure of mustard gas and there is a relationship between the increase of lymphocytes and dose of exposure.

El sulfuro de mostaza (SM), comúnmente conocido como gas mostaza, es un agente alquilante que causa graves ampollas en contacto con la piel humana. SM se utiliza con frecuencia como un agente de guerra química. Hay algunas evidencias que indican que el SM induce efectos en el sistema linfático en seres humanos. Entre los años 2000-2001, fueron utilizadas 42 ratas albinas Wistar macho. Después de la acomodación con el medio ambiente, las ratas se dividieron en grupos control, impostor y experimental (2,5 mg / kg, 5 mg / kg, 10 mg / kg, 20 mg / kg y 40 mg / kg). Luego se inyectó aceite de SM en el espacio intraperitoneal de las ratas. A continuación, sus bazos fueron removidos para la verificación histológica. Los resultados mostraron una diferencia significativa en el número de linfocitos en el grupo experimental después de 24 horas. El número de linfocitos en los grupos de 5, 10, 20 y 40 mg / kg fue mayor siendo este incremento en el grupo de 40 mg / kg más alto que en los otros grupos. Concluimos que el número de linfocitos aumenta debido a la exposición de gas mostaza existiendo una relación entre el aumento de linfocitos y la dosis de exposición.
Descritores: Baço
Gás de Mostarda/farmacologia
Leucócitos
-Relação Dose-Resposta a Droga
Gás de Mostarda/administração & dosagem
Ratos Wistar
Limites: Masculino
Animais
Ratos
Responsável: CL1.1 - Biblioteca Central


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Id: lil-387553
Autor: Vazquez, Raymond; Nelson, Marian R; Guzman, Juanita J; Corun, Charlene M; Steinberg, Mark.
Título: Immortalized human keratinocytes: A model system to study the efficacy of therapeutic drugs in response to the chemical warfare agent sulfur mustard (HD)
Fonte: Electron. j. biotechnol;7(2):124-129, Aug. 2004. graf.
Idioma: en.
Projeto: USA. U.S. Army Medical Research and Material Command; . USA. National Institutes of Health.
Resumo: Cytokines have been established as biomarkers to detect exposure of cells to chemical warfare agents such as sulfur mustard (2,2'-dichlorodiethyl sulfide, HD). In this study cultured normal and SV40 immortalized human epidermal keratinocyte (NHEK/IHEK) cells were compared as potential model systems to measure the efficacy of therapeutic drugs against HD. Immortalized human epidermal keratinocytes resemble their primary cell counterparts but have the advantage of being carried through long-term culture. Immortalized cells also provide consistency and durability and are less costly than primary keratinocytes. Immunoassay studies were performed to examine the response of these two cell lines to HD. We found that both normal and immortalized NHEKs secreted the pro-inflammatory mediator interleukin-8 (IL-8) when exposed to HD. However, a major difference was observed between the NHEK cell line 6207 and IHEK cell line 425. IHEK cell line 425 produced higher levels of Interleuken-8 then those of its normal counterpart cell line 6207. This observation is significant since therapeutic drugs such as ibuprofen, which depress cytokine production, may not allow these biomarkers to be detected efficiently in experimental analysis of certain NHEK cell lines. The fact that Il-8 production higher in cell line 425 cell makes this in vitro model a potential screening tool to study the efficacy of drugs that suppress production of cytokine markers.
Descritores: Substâncias para a Guerra Química
Gás de Mostarda
Queratinócitos
-Linhagem Celular
Sobrevivência Celular
Citocinas
Ensaio de Imunoadsorção Enzimática
Interleucinas
Modelos Biológicos
Biomarcadores
VIRUS ABRUPTIO PLACENTAE DE LOS SIMIOS
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central



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