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Id: biblio-839373
Autor: Guneser, Onur; Demirkol, Asli; Yuceer, Yonca Karagul; Togay, Sine Ozmen; Hosoglu, Muge Isleten; Elibol, Murat.
Título: Production of flavor compounds from olive mill waste by Rhizopus oryzae and Candida tropicalis
Fonte: Braz. j. microbiol;48(2):275-285, April.-June 2017. tab, graf.
Idioma: en.
Projeto: The Scientific and Technological Council of Turkey.
Resumo: Abstract The purpose of this study was to investigate the production of flavor compounds from olive mill waste by microbial fermentation of Rhizopus oryzae and Candida tropicalis. Olive mill waste fermentations were performed in shake and bioreactor cultures. Production of flavor compounds from olive mill waste was followed by Gas Chromatography–Mass spectrometry, Gas chromatography- olfactometry and Spectrum Sensory Analysis ®. As a result, 1.73-log and 3.23-log cfu/mL increases were observed in the microbial populations of R. oryzae and C. tropicalis during shake cultures, respectively. C. tropicalis can produce a higher concentration of d-limonene from olive mill waste than R. oryzae in shake cultures. The concentration of d-limonene was determined as 185.56 and 249.54 µg/kg in the fermented olive mill waste by R. oryzae and C. tropicalis in shake cultures respectively. In contrast, R. oryzae can produce a higher concentration of d-limonene (87.73 µg/kg) d-limonene than C. tropicalis (11.95 µg/kg) in bioreactor cultures. Based on sensory analysis, unripe olive, wet towel, sweet aromatic, fermented aromas were determined at high intensity in olive mill waste fermented with R. oryzae meanwhile olive mill waste fermented with C. tropicalis had only a high intensity of unripe olive and oily aroma.
Descritores: Rhizopus/metabolismo
Candida tropicalis/metabolismo
Olea/metabolismo
Aromatizantes/metabolismo
Resíduos Industriais
-Terpenos/metabolismo
Biotecnologia/métodos
Contagem de Colônia Microbiana
Cicloexenos/metabolismo
Fermentação
Olfatometria
Cromatografia Gasosa-Espectrometria de Massas
Responsável: BR1.1 - BIREME


  2 / 126 LILACS  
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Camargo, Zoilo Pires de
Moreira, Jose Luciano Bezerra
Rocha, Marcos Fábio Gadelha
Sidrim, José Júlio Costa
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Id: biblio-828189
Autor: Brilhante, Raimunda Sâmia Nogueira; Caetano, Érica Pacheco; Lima, Rita Amanda Chaves de; Marques, Francisca Jakelyne de Farias; Castelo-Branco, Débora de Souza Collares Maia; Melo, Charlline Vládia Silva de; Guedes, Glaucia Morgana de Melo; Oliveira, Jonathas Sales de; Camargo, Zoilo Pires de; Moreira, José Luciano Bezerra; Monteiro, André Jalles; Bandeira, Tereza de Jesus Pinheiro Gomes; Cordeiro, Rossana de Aguiar; Rocha, Marcos Fábio Gadelha; Sidrim, José Júlio Costa.
Título: Terpinen-4-ol, tyrosol, and ß-lapachone as potential antifungals against dimorphic fungi
Fonte: Braz. j. microbiol;47(4):917-924, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Projeto: CNPq; . CAPES.
Resumo: Abstract This study aimed to evaluate the in vitro antifungal activity of terpinen-4-ol, tyrosol, and β-lapachone against strains of Coccidioides posadasii in filamentous phase (n = 22) and Histoplasma capsulatum in both filamentous (n = 40) and yeast phases (n = 13), using the broth dilution methods as described by the Clinical and Laboratory Standards Institute, to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of these compounds. The mechanisms of action of these compounds were also investigated by analyzing their effect on cell membrane permeability and ergosterol synthesis. The MIC and MFCf these compounds against C. posadasii, mycelial H. capsulatum, and yeast-like H. capsulatum, were in the following ranges: 350-5720 µg/mL, 20-2860 µg/mL, and 40-1420 µg/mL, respectively for terpinen-4-ol; 250-4000 µg/mL, 30-2000 µg/mL, and 10-1000 µg/mL, respectively, for tyrosol; and 0.48-7.8 µg/mL, 0.25-16 µg/mL, and 0.125-4 µg/mL, respectively for β-lapachone. These compounds showed a decrease in MIC when the samples were subjected to osmotic stress, suggesting that the compounds acted on the fungal membrane. All the compounds were able to reduce the ergosterol content of the fungal strains. Finally, tyrosol was able to cause a leakage of intracellular molecules.
Descritores: Álcool Feniletílico/análogos & derivados
Terpenos/farmacologia
Naftoquinonas/farmacologia
Fungos/efeitos dos fármacos
Antifúngicos/farmacologia
-Pressão Osmótica
Álcool Feniletílico/farmacologia
Testes de Sensibilidade Microbiana
Permeabilidade da Membrana Celular/efeitos dos fármacos
Ergosterol/metabolismo
Fungos/classificação
Fungos/metabolismo
Responsável: BR1.1 - BIREME


  3 / 126 LILACS  
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Id: lil-623976
Autor: Zhou, Jun.
Título: Bioactive glycosides from Chinese medicines
Fonte: Mem. Inst. Oswaldo Cruz;86(supl.2):231-234, 1991. ilus.
Idioma: en.
Conferência: Apresentado em: Brazilian-Sino Symposium on Chemistry and Pharmacology of Natural Products, Rio de Janeiro, Dec. 10-14, 1989.
Resumo: Glycosides are the bioactive components of many famous Chinese medicines. Here reported are some bioactive glycosides we discovered from Chinese medicines in recent years. (1) Pheolic glycosides from Chinese medicines: Gastrodia elata, acontium austroynanense and Helicia erratica, three bioactive phenolic glycosides were discovered and two of them have been developed into new drugs. (2) Terpenoidal glycosides: a) Monoterpenoid: the sweroside from Swertia mollensis has been developed intro an anti-hepatitis drug; b) Diterpenoid: Phlomis betonicoides contains sweet glycoides; c) Triterpenoid: many biologically active triterpenoid glycosides were isolated from Panax plants and Siraitia grosvenorii. (3) Steroidal glycosides: a) C21-steroid: Cynanchum otophyllum and C. atratrum contain anti-epilepsy and-tumor glycosides; b) C27-steroid Hemostatic saponins were found in Paris polyphylla.
Descritores: Saponinas/isolamento & purificação
Esteroides/isolamento & purificação
Esteroides/farmacologia
Edulcorantes/isolamento & purificação
Terpenos/isolamento & purificação
Terpenos/farmacologia
Medicamentos de Ervas Chinesas/química
Glicosídeos/uso terapêutico
Hepatite/tratamento farmacológico
Antineoplásicos Fitogênicos/isolamento & purificação
-Anticonvulsivantes/isolamento & purificação
Limites: Seres Humanos
Animais
Responsável: BR1.1 - BIREME


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Id: lil-623930
Autor: Kaplan, Maria Auxiliadora C.
Título: Potential sources of biodynamically active natural products in Brazil
Fonte: Mem. Inst. Oswaldo Cruz;86(supl.2):5-8, 1991. ilus.
Idioma: en.
Conferência: Apresentado em: Brazilian-Sino Symposium on Chemistry and Pharmacology of Natural Products, Rio de Janeiro, Dec. 10-14, 1989.
Resumo: In contrast to China where vegatation is predominantly herbaceous, vegetation in Brazil is commonly arboreous. This fact may explain why Chinese drugs are usually acetate derived, while actual and potential natural therapeutic agents from Brazil are mostly shikimate derived. Only relatively few compounds isolated from Brazilian plants have been submitted to adequate pharmacological testing .
Descritores: Plantas Medicinais/classificação
Plantas Medicinais/metabolismo
Produtos Biológicos/síntese química
Produtos Biológicos/química
-Terpenos
Brasil
Lignanas
Ecologia
Responsável: BR1.1 - BIREME


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Id: biblio-1021049
Autor: Lei, Chao; Hao, Ruijuan; Zheng, Zhe; Deng, Yuewen; Wang, Qingheng; Li, Junhui.
Título: Molecular cloning and characterisation of scavenger receptor class B in pearl oyster Pinctada fuctada martensii
Fonte: Electron. j. biotechnol;30:12-17, nov. 2017. tab, graf.
Idioma: en.
Projeto: Graduate Education Innovation Program of Guangdong Ocean University; . Administration of Ocean and Fisheries of Guangdong Province; . Innovation Strong School Project of Guangdong Province.
Resumo: Background: Molluscs can accumulate carotenoids in their body tissues by predominantly feeding on aquatic plant sources. Carotenoid transport and absorption are determined by the regulation of various proteins such as Scavenger receptor class B(SR-BI). We report the identification and characterisation of pearl oyster Pinctada fuctada martensii SR-BI (PmSR-BI). The correlation between total carotenoid content (TCC) and gene expression was also estimated. Results: The full-length cDNA of PmSR-BI was 1828 bp, including an open-reading frame encoding of 1518 bp with a pI value of 5.83. PmSR-BI protein contains a hydrophobic CD36 domain and four centrally clustered cysteine residues for the arrangement of disulphide bridges. The deduced amino acid sequence had an identity of 30% to 60% with the SR-B of other organisms. Reverse transcription polymerase chain reaction analysis showed that mRNA transcripts were expressed in multiple tissues of adult pearl oyster. A higher expression of PmSR-BI gene was observed in the hepatopancreas than in the adductor muscle, gill and mantle. The TCC and gene expression of PmSR-BI were significantly correlated (P b 0.05), with a correlation coefficient of 0.978. Conclusions: The results suggested that PmSR-BI is involved in the absorption of carotenoids in the pearl oyster P. fuctada martensii.
Descritores: Carotenoides/metabolismo
Pinctada
Receptores Depuradores/genética
Receptores Depuradores/metabolismo
-Terpenos
Vitamina A/metabolismo
RNA Mensageiro/genética
Expressão Gênica
Clonagem Molecular
Análise de Sequência
Ácido Abscísico
DNA Complementar/genética
Interações Hidrofóbicas e Hidrofílicas
Reação em Cadeia da Polimerase em Tempo Real
Responsável: CL1.1 - Biblioteca Central


  6 / 126 LILACS  
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Id: biblio-1015852
Autor: Yan, Kuan; Wei, Qin; Feng, Ruizhang; Zhou, Wanhai; Chen, Fang.
Título: Transcriptome analysis of Cinnamomum longepaniculatum by high-throughput sequencing
Fonte: Electron. j. biotechnol;28:58-66, July. 2017. tab, graf, ilus.
Idioma: en.
Projeto: Sichuan Education Department; . Innovative Program of Sichuan Undergraduates; . Key Lab of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education.
Resumo: Background: Cinnamomum longepaniculatum is an important commercial crop and the main source of volatile terpenoids. The biosynthesis of key bioactive metabolites of C. longepaniculatum is not well understood because of the lack of available genomic and transcriptomic information. To address this issue, we performed transcriptome sequencing of C. longepaniculatum leaves to identify factors involved in terpenoid metabolite biosynthesis. Results: Transcriptome sequencing of C. longepaniculatum leaves generated over 56 million raw reads. The transcriptome was assembled using the Trinity software and yielded 82,061 unigenes with an average length of 879.43 bp and N50 value of 1387 bp. Furthermore, Benchmarking Universal Single-Copy Orthologs analysis indicated that our assembly is 91% complete. The unigenes were used to query the nonredundant database depending on sequence similarity; 42,809 unigenes were homologous to known genes in different species, with an annotation rate of 42.87%. The transcript abundance and Gene Ontology analyses revealed that numerous unigenes were associated with metabolism, while others were annotated in functional categories including transcription, signal transduction, and secondary metabolism. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that 19,260 unigenes were involved in 385 metabolic pathways, with 233 unigenes found to be involved in terpenoid metabolism. Moreover, 23,463 simple sequence repeats were identified using the microsatellite identification tool. Conclusion: This is the first detailed transcriptome analysis of C. longepaniculatum. The findings provide insights into the molecular basis of terpenoid biosynthesis and a reference for future studies on the genetics and breeding of C. longepaniculatum.
Descritores: Terpenos/metabolismo
Cinnamomum/genética
Sequenciamento de Nucleotídeos em Larga Escala
Transcriptoma
-Transcrição Genética
Cruzamento
Óleos Voláteis/metabolismo
Repetições de Microssatélites
Anotação de Sequência Molecular
Ontologia Genética
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1008263
Autor: Rodríguez-Hernández, Diego.
Título: Secondary metabolites as a survival strategy in plants of high mountain habitats / Metabolitos secundarios como estrategia de supervivencia en plantas de hábitat de alta montaña
Fonte: Bol. latinoam. Caribe plantas med. aromát;18(5):444-458, sept. 2019. ilus, tab.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: This review work focuses on how the secondary chemistry could help in the survival of plants in high mountain habitats under extreme environmental conditions. The elevated levels of stress in high areas of the tropic and subtropic change dramatically not only by following the annual cycles of winter and summer but they also change in a single day. Some species, however, are able to successfully grow at heights more than 3000 m in the tropical mountains due, in part, to highly specialized physiological processes that affect their physical and chemical responses. In this study, it describes some strategies of how the secondary metabolites could help the plants to stand the high levels of stress in the high mountain ecosystems.

Este trabajo de revisión se centra en cómo la química secundaria podría ayudar en la supervivencia de plantas en hábitats de alta montaña en condiciones ambientales extremas. Los altos niveles de estrés en las zonas altas del trópico y subtrópico cambian dramáticamente no solo al seguir los ciclos anuales de invierno y verano, sino que también cambian en un solo día. Sin embargo, algunas especies pueden crecer con éxito a alturas superiores a 3000 m en las montañas tropicales debido, en parte, a procesos fisiológicos altamente especializados que afectan sus respuestas físicas y químicas. En esta revisión, se describen algunas estrategias de cómo los metabolitos secundarios podrían ayudan a las plantas a soportar los altos niveles de estrés en los ecosistemas de alta montaña.
Descritores: Plantas/química
Estresse Fisiológico
Óleos Voláteis/química
Altitude
-Floroglucinol/análise
Floroglucinol/química
Terpenos/análise
Terpenos/química
Flavonoides/análise
Flavonoides/química
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


  8 / 126 LILACS  
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Id: biblio-1008180
Autor: Centorbi, Hugo José; Aliendro, Olga Elida; Mattana, Claudia Maricel.
Título: Effect of thymol and environmental factors on growth and biofilm formation by Listeria monocytogenes / Efecto del timol y factores ambientales sobre el crecimiento y formación de biopelícula por Listeria monocytogenes
Fonte: Bol. latinoam. Caribe plantas med. aromát;18(4):411-424, jul. 2019. tab, ilus.
Idioma: en.
Projeto: National University of San Luis.
Resumo: Thymol (2-isopropyl-5-methylphenol) is an aromatic monoterpene found in essential oils extracted from plants belonging to the Lamiaceae family, such as Thymus, Ocimum, Origanum, Satureja, Thymbra and Monarda genera. Growth and biofilm formation by Listeria monocytogenes CLIP 74902 were evaluate using three carbon sources in the presence of thymol. Specific growth rate (h-1) values at 37o with glucose, trehalose and cellobiose with the addition of thymol (µg/mL) 0 (control) and 750, were respectively: 0.22, 0.07; 0.14, 0.04; 0.11, 0.04. Lag periods obtained under the same conditions were (h): 8.19, 13.2; 22.5, 27.5; 23.1, 28.1. A marked antibiofilm activity was observed against the exposure with 750 µg/mL of thymol, showing a high percentage of inhibition: glucose (99 %), trehalose (97 %) and cellobiose (98%), compared to the control. The results suggest that thymol could be used to inhibit the growth and production of biofilms by L. monocytogenes in the food industry.

Timol (2-isopropil-5-metilfenol) es un monoterpeno aromático presente en los aceites esenciales extraídos de plantas pertenecientes a la familia Lamiaceae, como los géneros Thymus, Ocimum, Origanum, Satureja, Thymbra y Monarda. El crecimiento y formación de biopelícula por Listeria monocytogenes CLIP 74902 fueron evaluados utilizando tres fuentes de carbono en presencia de timol. La velocidad específica de crecimiento (h-1) a 37o con glucosa, trehalosa y celobiosa con la adición de timol (µg/mL) 0 (control) y 750, fueron respectivamente: 0.22, 0.07; 0.14, 0.04, 0.11, 0,04. Los períodos lag obtenidos en las mismas condiciones fueron (h): 8.19, 13.2; 22.5, 27.5; 23.1, 28.1. Una marcada actividad antibiofilm fue obtenida con 750 µg/mL de timol, mostrando un alto porcentaje de inhibición con glucosa (99%), trehalosa (97%) y celobiosa (98%), respecto al control. Los resultados sugieren que timol podría ser usado para inhibir el crecimiento y producción de biopelículas por L. monocytogenes en la industria alimentaria.
Descritores: Timol/farmacologia
Biofilmes/efeitos dos fármacos
Listeria monocytogenes/efeitos dos fármacos
-Terpenos/farmacologia
Cinética
Biofilmes/crescimento & desenvolvimento
Meio Ambiente
Fermentação
Microbiologia de Alimentos
Listeria monocytogenes/crescimento & desenvolvimento
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1007341
Autor: Valdés, Claudio; Valenzuela, Beatriz; Modak, Brenda.
Título: Evaluation in vitro of proliferative activity of epithelial cells by flavonoid 3-O-methylgalangine and terpenenic derivative Filifolinone / Evaluación in vitro de la actividad proliferative de células epiteliales del flavonoide 3-O-metilgalangina y el derivado terpénico Filifolinona
Fonte: Bol. latinoam. Caribe plantas med. aromát;17(6):575-582, nov. 2018. ilus.
Idioma: en.
Projeto: Fondecyt.
Resumo: The skin is the largest organ of the human body and its main function is to protect it from the external environment. It is exposed to injuries that require a rapid healing process to recover its functionality. Microorganisms inhabit the skin, which makes up the normal microbial flora, but in situations of injury they can cause infections that slow down the regeneration process. Therefore, there is a great interest in the development of alternative methods to accelerate the regeneration process and prevent infections. In this work, the efficacy of flavonoid 3-O-methylgalangine and the terpenic derivative Filifolinone and its mixtures, isolated from plants of the genus Heliotropium, on the stimulation of cell proliferation was evaluated. The results showed that the mixtures stimulated proliferation and migration in MA104 cells mainly due to the presence of Filifolinone, that together with the known antibacterial activity of 3-O-methylgalangine, opens new alternatives for the use of natural compounds in healing processes.

La piel es el órgano más grande del cuerpo humano y su función principal es protegerla del entorno externo. Está expuesta a lesiones que requieren un proceso de curación rápido para recuperar su funcionalidad. Los microorganismos que habitan en la piel, constituyen la flora microbiana normal, pero en situaciones de lesión pueden causar infecciones que retardan el proceso de regeneración. Por lo tanto, existe un gran interés en el desarrollo de métodos alternativos para acelerar el proceso de regeneración y prevenir infecciones. En este trabajo, se evaluó la eficacia del flavonoide 3-O-metilgalangina y el derivado terpénico Filifolinona y sus mezclas, aisladas de plantas del género Heliotropium, en la estimulación de la proliferación celular. Los resultados mostraron que las mezclas estimularon la proliferación y la migración en las células MA104 debido principalmente a la presencia de Filifolinona, que junto con la actividad antibacteriana conocida de la 3-O-metilgalangina, abre nuevas alternativas para el uso de compuestos naturales en los procesos de curación.
Descritores: Terpenos/farmacologia
Flavonoides/farmacologia
Heliotropium
Proliferação Celular/efeitos dos fármacos
-Terpenos/química
Cicatrização
Flavonoides/química
Movimento Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Células Epiteliais/efeitos dos fármacos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-915398
Autor: Pereira, Evaristo José P; Silva, Horlando C; Holanda, Carlos L; Menezes, Jane Eire Silva A. de; Siqueira, Sônia Maria C; Rodrigues, Tigressa Helena S; Fontenelle, Raquel Oliveira. S; Vale, Jean Parcelli C. do; Silva, Priscila T. da; Santiago, Gilvandete Maria P; Santos, Hélcio S.
Título: Chemical composition, cytotoxicity and larvicidal activity against Aedes aegypti of essential oils from Vitex gardineriana Schauer / Composición química, citotoxicidad y actividad larvicida contra Aedes aegypti de aceites esenciales de Vitex gardneriana Schauer
Fonte: Bol. latinoam. Caribe plantas med. aromát;17(3):302-309, mayo 2018. ilus, tab.
Idioma: en.
Projeto: Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico; . Conselho Nacional de Desenvolvimento Científico e Tecnológico; . EMBRAPA AGROINDÚSTRIA TROPICAL.
Resumo: Vitex gardneriana Schauer (Lamiaceae) popularly known as "jaramataia", is a shrub commonly found in caatinga biome located in Northeast Brazil. In folk medicine, its leaves have been used as analgesic and anti-inflammatory agents. The chemical composition of the essential oil from leaves obtained by hydrodistillation was analyzed and identified by GC-MS and GC-FID and showing a total of 26 constituents (95.9%) being 2 monoterpenes (0.4%) and 24 sesquiterpenes (95.4%). The main constituents identified were cis-calamenene (29.7%), 6,9-guaiadiene (14.5%) and caryophyllene oxide (14.0%). The essential oil has been demonstrated high larvicidal activity against Aedes aegypti (LC50 = 28.0 µg/mL). In the evaluation of the bioassay with Artemia salina the essential oil showed LC50 = 98.11 µg/mL. In addition, the essential oil did not show cytotoxicity (IC50 > 2.50 mg/mL) by the hemolysis assay.

Vitex gardneriana Schauer (Lamiaceae) popularmente conocido como "jaramataia", es un arbusto que se encuentra comúnmente en el bioma de caatinga ubicado en el noreste de Brasil. En medicina popular, sus hojas se han utilizado como analgésicos y agentes antiinflamatorios. La composición química de los aceites esenciales de las hojas obtenidas por hidrodestilación fue analizada e identificada por GC-MS y GC-FID y mostrando un total de 26 constituyentes (95.9%) siendo 2 monoterpenos (0.4%) y 24 sesquiterpenos (95.4%). Los componentes principales fueron cis-calamenene (29.7%), 6,9-guaiadiene (14.5%) y caryophyllene oxide (14.0%). El aceite esencial ha demostrado una alta actividad larvicida contra Aedes aegypti (CL50 = 28.0 µg/mL). En la evaluación del bioensayo con Artemia salina, el aceite esencial demostró CL50 = 98.11 µg/mL. Además, el aceite esencial no mostró citotoxicidad (IC50 > 2.5 mg / mL) mediante el ensayo de hemólisis.
Descritores: Óleos Voláteis/farmacologia
Extratos Vegetais/farmacologia
Folhas de Planta/química
Aedes/efeitos dos fármacos
Vitex/química
-Terpenos/análise
Óleos Voláteis/química
Extratos Vegetais/química
Cromatografia Gasosa
Larvicidas
Larva
Responsável: CL1.1 - Biblioteca Central



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