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Id: biblio-974167
Autor: Tonon, Caroline Coradi; Francisconi, Renata Serignoli; Bordini, Ester Alves Ferreira; Huacho, Patrícia Milagros Maquera; Department of Physiological SciencesSardi, Janaína de Cássia Orlandi; Spolidorio, Denise Madalena Palomari.
Título: Interactions between Terpinen-4-ol and Nystatin on biofilm of Candida albicans and Candida tropicalis
Fonte: Braz. dent. j;29(4):359-367, July-Aug. 2018. tab, graf.
Idioma: en.
Projeto: The São Paulo Research Foundation - FAPESP.
Resumo: Abstract The aim of this study was to evaluate the antifungal activity of Terpinen-4-ol associated with nystatin, on single and mixed species biofilms formed by Candida albicans and Candida tropicalis, as well as the effect of terpinen-4-ol on adhesion in oral cells and the enzymatic activity. The minimum inhibitory concentrations and minimum fungicide concentrations of terpinen-4-ol and nystatin on Candida albicans and Candida tropicalis were determined using the microdilution broth method, along with their synergistic activity ("checkerboard" method). Single and mixed species biofilms were prepared using the static microtiter plate model and quantified by colony forming units (CFU/mL). The effect of Terpinen-4-ol in adhesion of Candida albicans and Candida tropicalis in coculture with oral keratinocytes (NOK Si) was evaluated, as well as the enzymatic activity by measuring the size of the precipitation zone, after the growth agar to phospholipase, protease and hemolysin. Terpinen-4-ol (4.53 mg mL-1) and nystatin (0.008 mg mL-1) were able to inhibit biofilms growth, and a synergistic antifungal effect was showed with the drug association, reducing the inhibitory concentration of nystatin up to 8 times in single biofilm of Candida albicans, and 2 times in mixed species biofilm. A small decrease in the adhesion of Candida tropicalis in NOK Si cells was showed after treatment with terpinen-4-ol, and nystatin had a greater effect for both species. For enzymatic activity, the drugs showed no action. The effect potentiated by the combination of terpinen-4-ol and nystatin and the reduction of adhesion provide evidence of its potential as an anti-fungal agent.

Resumo O objetivo desse estudo foi avaliar a atividade antifúngica do Terpinen4-ol associado à nistatina em biofilmes simples e misto, formados por Candida albicans e Candida tropicalis, bem como o efeito do terpinen-4-ol na adesão em células orais e atividade enzimática. As concentrações inibitórias mínimas e as concentrações fungicidas mínimas do terpinen-4-ol e da nistatina em Candida albicans e Candida tropicalis foram determinadas pelo método de microdiluição em caldo, juntamente com a atividade sinérgica (método do tabuleiro de "xadrez"). Biofilmes simples e misto foram preparados usando o modelo de placa de microtitulação estática e quantificados por unidades formadoras de colônias (CFU/mL). O efeito do Terpinen-4-ol na adesão de Candida albicans e Candida tropicalis em co-cultura com queratinócitos orais (NOK Si) foi avaliado, bem como a atividade enzimática, medindo o tamanho da zona de precipitação, após o crescimento em ágar fosfolipase, protease e hemolisina. O terpinen-4-ol (4.53 mg mL-1) e a nistatina (0,008 mg mL-1) conseguiram inibir o crescimento de biofilmes e um efeito antifúngico sinérgico foi demonstrado com a associação de fármaco, reduzindo a concentração inibidora de nistatina até 8 vezes em biofilme simpes de Candida albicans e 2 vezes em biofilme misto. Uma pequena diminuição na adesão de Candida tropicalis em células NOK Si foi mostrada após o tratamento com terpinen-4-ol e a nistatina teve um efeito maior para ambas as espécies. Para a atividade enzimática, as drogas não apresentaram ação. O efeito potencializado pela combinação de terpinen-4-ol e nistatina e a redução de adesão evidenciam seu potencial como agente anti-fúngico.
Descritores: Terpenos/farmacologia
Candida albicans/efeitos dos fármacos
Nistatina/farmacologia
Biofilmes/efeitos dos fármacos
Candida tropicalis/efeitos dos fármacos
Antifúngicos/farmacologia
-Linhagem Celular Transformada
Testes de Sensibilidade Microbiana
Sinergismo Farmacológico
Responsável: BR1.1 - BIREME


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Id: biblio-1342818
Autor: da Camara, Claudio AG; de Araujo, Carolina A; de Moraes, Marcilio M; de Melo, João PR; Lucena, Maria FA.
Título: New sources of botanical acaricides from species of Croton with potential use in the integrated management of Tetranychus urticae / Nuevas fuentes de acaricidas botánicos de especies de Croton con uso potencial en el manejo integrado de Tetranychus urticae
Fonte: Bol. latinoam. Caribe plantas med. aromát;20(3):244-259, may. 2021. tab, ilus.
Idioma: en.
Resumo: Essential oils from the leaves and stems of Croton adenocalyx, C. grewioides, C. heliotropiifoliusand C. blanchetianus obtained through hydrodistillation were analyzed by GC-MS. We then investigated the lethal and sublethal effects of the Croton oils and 15 major constituents against Tetranychus urticae. ß-Caryophyllene was the major component in the leaf and stem oils from C. heliotropiifolius and C. adenocalyx. Spathulenol and (E)-anethole were the major constituents identified in the leaf and stem oils of C. blanchetianus and C. grewioides, respectively. The oil with the greatest lethal and sublethal effects was those from C. adenocalyx. Among the constituents ß-caryophyllene and spatulenol were the most toxic to the mite, where as eugenol and methyl eugenol were the most repellent. The toxicity and repellency of the Croton oils, particularly the oils from C. adenocalyx, demonstrate that these oils constitute a promising alternative to synthetic acaricides for use in the control of T. urticae.

Los aceites esenciales de las hojas y tallos de Croton adenocalyx, C. growioides, C. heliotropiifolius y C. blanchetianus obtenidos mediante hidrodestilación fueron analizados a través de GC-MS. Se investigaron los efectos letales y subletales de los aceites de Croton y 15 componentes principales contra Tetranychus urticae. El ß-cariofileno fue el componente principal en los aceites de hojas y tallos de C. heliotropiifolius y C. adenocalyx. El espatulenol y el (E)-anetol fueron los principales componentes identificados en los aceites de hojas y tallos de C. blanchetianus y C. growioides, respectivamente. El aceite con los mayores efectos letales y subletales fue el de C. adenocalyx. Entre los componentes, el ß-cariofileno y el espatulenol fueron los más tóxicos para el ácaro, mientras que el eugenol y el metil eugenol fueron los más repelentes. La toxicidad y la repelencia de los aceites de Croton, particularmente los aceites de C. adenocalyx, demuestran que estos aceites constituyen una alternativa prometedora respecto a los acaricidas sintéticos para uso en el control de T. urticae.
Descritores: Óleos Voláteis/farmacologia
Croton/química
Tetranychidae/efeitos dos fármacos
Acaricidas/farmacologia
-Terpenos/análise
Óleos Voláteis/química
Cromatografia Gasosa/métodos
Folhas de Planta
Acaricidas/química
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1342813
Autor: Henriques, Martha Quitéria Silva; Barbosa, David Henrique Xavier; Alves, Danielle da Nóbrega; Melo, Ana Karoline Vieira; Castro, Ricardo Dias de.
Título: Chemical composition, antioxidant, antimicrobial activity, toxicity, genetic analysis and popular use of Eugenia luschnathiana (O. Berg) Klotzsch ex B. D. Jacks: a literature review / Composición química, actividad antioxidante, antimicrobiana, toxicidad, análisis genético y uso popular de Eugenia luschnathiana (O. Berg) Klotzsch ex B. D Jack: una revisión de la literatura
Fonte: Bol. latinoam. Caribe plantas med. aromát;20(3):215-225, may. 2021. ilus, tab.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Resumo: This review describes the geographical distribution, botanical data, popular use, chemical composition, pharmacological activities and genetic aspects related to Eugenia luschnathiana, a native Brazilian plant popularly known as "bay pitomba". E. luschnathiana leaves are characterized morphologically by the presence of a petiole, an attenuated base, acuminated apex, elliptical shape, and parallel venation. The major chemical compounds found in E. luschnathiana are sesquiterpenes. Literature reports showed that E. luschnathiana extracts have antioxidant properties and antimicrobial activity against Gram-negative and Gram-positive bacteria. The extractsfrom the leaf, fruit and stem, and a concentrated residual solution of its essential oil, displayed negligible toxicity. Lastly, a cytogenetic analysis indicated that some markers can be used for the study of genetic diversity, population structure, and genetic improvements. The information available on E. luschnathiana supports the hypothesis that this plant may be a source of compounds with promising pharmacological activity.

Esta revisión describe la distribución geográfica, datos botánicos, uso popular, composición química, actividad farmacológica y el análisis genético de Eugenia luschnathiana, una planta originaria del Brasil conocida popularmente como "pitomba da baía". Las hojas de E. luschnathiana se caracterizan por la presencia de pecíolo, base atenuada, ápice acuminado, forma elíptica y venación paralela. Su composición química presenta mayormente sesquiterpenos. Los informes en la literatura muestran que los extractos de E. luschnathiana presentan propiedades antioxidantes y actividad antimicrobiana contra las bacterias Gram-negativas y Gram-positivas. Los extractos de la hoja, fruto y tallo, y una solución residual concentrada del aceite esencial, presentaron baja toxicidad. Por último, un análisis citogenético indicó que algunos marcadores pueden utilizarse para estudios de diversidad genética, estructura poblacional y mejoramiento genético. Las informaciones disponibles acerca de E. luschnathiana proponen la hipótesis de que esta planta puede ser una fuente de compuestos con actividad farmacológica prometedora.
Descritores: Óleos Voláteis/farmacologia
Extratos Vegetais/farmacologia
Eugenia/química
Anti-Infecciosos/farmacologia
-Terpenos/análise
Bactérias/efeitos dos fármacos
Óleos Voláteis/química
Extratos Vegetais/genética
Extratos Vegetais/química
Folhas de Planta/química
Eugenia/genética
Medicina Tradicional
Anti-Infecciosos/química
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-952002
Autor: Nassar, Hani; Chu, Tien-Min; Platt, Jeffrey.
Título: Optimizing light-cured composite through variations in camphorquinone and butylhydroxytoluene concentrations
Fonte: Braz. oral res. (Online);30(1):e66, 2016. tab, graf.
Idioma: en.
Resumo: Abstract The use of a free-radical polymerization inhibitor, butylhydroxytoluene (BHT), and a common photo-initiator, camphorquinone (CQ), to reduce polymerization stress in dental composite was investigated in this study. Samples were prepared by mixing Bis-GMA, UDMA, and TEGDMA at a 1:1:1 ratio (wt%), and silanized borosilicate glass fillers at 70 wt% were added to form the composite. Sixteen groups of resin composite were prepared using combinations of four CQ (0.1%, 0.5%, 1.0%, and 1.5%) and four BHT (0.0%, 0.5%, 1.0%, and 1.5%) concentrations. For each group, six properties were tested, including flexural strength (FS), flexural modulus (FM), degree of conversion (DC), contraction stress (CS), stress rate, and gel point (GP). The effects of CQ and BHT combinations on each of these properties were evaluated using two-way analysis of variance (ANOVA) and Fisher's Protected Least Significant Differences test at the 5% significance level. Groups with low CQ and BHT showed moderate values for FS, FM, and CS with a 70% DC. Increasing the BHT concentration caused a decrease in CS and DC with an increase in GP values. Increasing the CQ content led to a steady increase in values for FS and FM. High CQ and BHT combinations showed the most promising values for mechanical properties with low stress values.
Descritores: Terpenos/química
Hidroxitolueno Butilado/química
Resinas Compostas/química
Luzes de Cura Dentária
-Polietilenoglicóis/química
Ácidos Polimetacrílicos/química
Poliuretanos/química
Valores de Referência
Estresse Mecânico
Fatores de Tempo
Teste de Materiais
Análise de Variância
Bis-Fenol A-Glicidil Metacrilato/química
Maleabilidade
Módulo de Elasticidade
Polimerização
Metacrilatos/química
Responsável: BR1.1 - BIREME


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Id: biblio-1132678
Autor: Costa, Tereza Karla Vieira Lopes da; Barros, Mariana Silva; Braga, Renan Marrinho; Viana, Jéssika de Oliveira; Sousa, Frederico Barbosa de; Scotti, Luciana; Scotti, Marcus Tullius; Batista, André Ulisses Dantas; Almeida, Reinaldo Nóbrega de; Castro, Ricardo Dias de.
Título: Orofacial antinociceptive activity and anchorage molecular mechanism in silico of geraniol
Fonte: Braz. oral res. (Online);34:e094, 2020. tab, graf.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Resumo: Abstract We aimed to evaluate the orofacial antinociceptive effect of geraniol in mice and its molecular anchorage mechanism. Seven mice per group (probabilistic sample) were treated with geraniol (12.5, 25 and 50 mg/kg, i.p.), morphine (6 mg/kg, i.p.) and vehicle (saline + Tween 80 at 0.2%, i.p.) 30 minutes prior to the beginning of the experiment. Injecting glutamate (25 μM), capsaicin (2.5 μg) and formalin (2%) into the right upper lip (perinasal) of the mouse induced nociception. Behavioral analysis of the animals considered the friction time (in seconds) of the mentioned region using hind or front paws by a researcher blinded to the treatment groups. The statistical analysis was performed blindly, considering α = 5%. The results showed that in the glutamate and capsaicin tests, concentrations of 25 mg/kg and 50 mg/kg presented antinociceptive activity (p < 0.005, power> 80%). In the formalin test, geraniol was able to reduce nociception at a concentration of 50 mg/kg (p < 0.005, power> 80%). In the molecular anchorage study, high values of binding between the evaluated substance and receptors of glutamate were observed (metabotropic glutamate receptor, -87.8501 Kcal/mol; N-methyl-D-aspartate, -86.4451 Kcal/mol; α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid, -85.6755 Kcal/mol). Geraniol presented orofacial antinociceptive activity, probably by interacting with glutamate-related receptors.
Descritores: Dor Facial
-Terpenos
Medição da Dor
Monoterpenos Acíclicos
Analgésicos
Limites: Animais
Camundongos
Responsável: BR1.1 - BIREME


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Id: biblio-1132693
Autor: Francisconi, Renata Serignoli; Huacho, Patricia Milagros Maquera; Tonon, Caroline Coradi; Bordini, Ester Alves Ferreira; Correia, Marília Ferreira; Sardi, Janaína de Cássia Orlandi; Spolidorio, Denise Madalena Palomari.
Título: Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans
Fonte: Braz. oral res. (Online);34:e050, 2020. graf.
Idioma: en.
Projeto: Fapesp.
Resumo: Abstract Candida infection is an important cause of morbidity and mortality in immunocompromised patients. The increase in its incidence has been associated with resistance to antimicrobial therapy and biofilm formation. The aim of this study was to evaluate the efficacy of tea tree oil (TTO) and its main component - terpinen-4-ol - against resistant Candida albicans strains (genotypes A and B) identified by molecular typing and against C. albicans ATCC 90028 and SC 5314 reference strains in planktonic and biofilm cultures. The minimum inhibitory concentration, minimum fungicidal concentration, and rate of biofilm development were used to evaluate antifungal activity. Results were obtained from analysis of the biofilm using the cell proliferation assay 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and confocal laser scanning microscopy (CLSM). Terpinen-4-ol and TTO inhibited C. albicans growth. CLSM confirmed that 17.92 mg/mL of TTO and 8.86 mg/mL of terpinen-4-ol applied for 60 s (rinse simulation) interfered with biofilm formation. Hence, this in vitro study revealed that natural substances such as TTO and terpinen-4-ol present promising results for the treatment of oral candidiasis.
Descritores: Terpenos/farmacologia
Candida albicans/efeitos dos fármacos
Biofilmes/efeitos dos fármacos
Óleo de Melaleuca/farmacologia
-Valores de Referência
Terpenos/química
Resinas Acrílicas
Candida albicans/crescimento & desenvolvimento
Testes de Sensibilidade Microbiana
Reprodutibilidade dos Testes
Análise de Variância
Estatísticas não Paramétricas
Microscopia Confocal
Biofilmes/crescimento & desenvolvimento
Óleo de Melaleuca/química
Bases de Dentadura/microbiologia
Antifúngicos/farmacologia
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-1284405
Autor: Raimundo, Keila Fernanda; Bortolucci, Wanessa de Campos; Rahal, Isabelle Luiz; de Oliveira, Hérika Line Marko; Júnior, Ranulfo Piau; Campo, Caio Franco de Araújo Almeida; Gonçalves, José Eduardo; Linde, Giani Andrea; Colauto, Nelson de Barros; Gazim, Zilda Cristiani.
Título: Insecticidal activity of Gallesia integrifolia (Phytolaccaceae) essential oil / Actividad insecticida del aceite esencial de Gallesia integrifolia (Phytolaccaceae)
Fonte: Bol. latinoam. Caribe plantas med. aromát;20(1):38-50, 2021. tab, ilus.
Idioma: en.
Resumo: This study evaluated the insecticidal activity of Gallesia integrifolia essential oil from fruits, leaves, and flowers against Aedes aegyptilarvae and pupae. The essential oil was obtained by hydrodistillation and characterized by gas chromatography-mass spectrometry. Sulfur compounds represented 95 to 99% of the essential oil from fruits, leaves, and flowers. Essential oil major compounds were 2,8-dithianonane (52.6%) in fruits, 3,5-dithiahexanol-5,5-dioxide (38.9%) in leaves, and methionine ethyl ester (45.3%) in flowers. The essential oils showed high activity against larvae, and low for pupae withLC99.9of 5.87 and 1476.67µg/mL from fruits; 0.0096 and 348.33 µg/mL from leaves and 0.021and 342.84 µg/mL from flowers, respectively. The main compound with insecticide activity is probably n-ethyl-1,3-dithioisoindole, from isoindole organothiophosphate class, found in greater amount in flower and leaf essential oil. The great insecticide activity of G. integrifolia essential oil suggests that this product is a natural insecticide.

Este estudio evaluó la actividad insecticida del aceite esencial de frutos, hojas y flores de Gallesia integrifolia contra larvas y pupas de Aedes aegypti. El aceite esencial se obtuvo por hidrodestilación y se caracterizó por cromatografía de gases acoplada a espectrometría de masas. Los compuestos de azufre representaron del 95 al 99% del aceite esencial de frutas, hojas y flores. Los compuestos principales del aceite esencial fueron 2,8-ditianonano (52,6%) en frutas, 3,5-ditiahexanol-5,5-dióxido (38,9%) en hojas y éster etílico de metionina (45,3%) en flores. Los aceites esenciales mostraron alta actividad contra larvas y baja para pupas con LC99.9de 5.87 y 1476.67µg/mL de frutos; 0,0096 y 348,33 µg/mL de hojas y 0,021 y342,84 µg/mL de flores, respectivamente. El principal compuesto con actividad insecticida es probablemente el n-etil-1,3-ditioisoindol, de la clase de los organotiofosfatos de isoindol, que se encuentra en mayor cantidad en el aceite esencial de flores y hojas. La gran actividad insecticida del aceite esencial de G. integrifolia sugiere que este producto es un insecticida natural.
Descritores: Óleos Voláteis/farmacologia
Aedes/efeitos dos fármacos
Phytolaccaceae/química
Inseticidas/farmacologia
-Terpenos/análise
Óleos Voláteis/química
Inibidores da Colinesterase
Folhas de Planta/química
Flores/química
Frutas/química
Inseticidas/química
Cromatografia Gasosa-Espectrometria de Massas
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1278459
Autor: Pauzer, Matheus Saukoski; Borsato, Thais de Oliveira; Almeida, Valter Paes de; Raman, Vijayasankar; Justus, Bárbara; Pereira, Camila Bugnotto; Flores, Thiago Bevilacqua; Maia, Beatriz Helena Lameiro Noronha Sales; Meneghetti, Emanuelle Kretz; Kanunfre, Carla Cristine; Paula, Josiane de Fátima Padilha de; Farago, Paulo Vitor; Budel, Jane Manfron.
Título: Eucalyptus cinerea: Microscopic Profile, Chemical Composition of Essential Oil and its Antioxidant, Microbiological and Cytotoxic Activities
Fonte: Braz. arch. biol. technol;64(spe):e21200772, 2021. tab, graf.
Idioma: en.
Resumo: Abstract Eucalyptus species possess anti-inflammatory, antifungal, antibacterial, and insecticidal properties. In this study, the chemical composition and biological activities of Eucalyptus cinerea essential oil (EO) and the leaf and stem anatomy were investigated. EO was extracted by Clevenger apparatus and the compounds were identified by GC/MS. The antioxidant activity was evaluated by DPPH, ABTS, and reducing phosphomolybdenum complex. Broth microdilution was used to determine antimicrobial activity. Cytotoxicity was verified against HeLa, HRT-18, and Calu-3 cells by MTT assay. The cytotoxic mechanism was studied by cell DNA content, cell cycle, and DNA fragmentation. The microscopic analyzes of the leaves and the stems were performed by light microscopy, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The main constituent of the EO was 1,8-cineole (55.24%). The EO showed low antioxidant and antimicrobial activities. Calu-3 cells showed a significant reduction in viability with IC50 of 689.79 ± 29.34 μg/mL. EO at 1000 μg/mL decreased the DNA content in Jurkat cells. In general, EO increased cell percentage in sub-G0 and S phases with concomitant reduction of cell percentage in G0/G1 and G2/M phases and provided DNA fragmentation of 29.73%. Anatomical and micromorphological features of the leaves and stems can help in the species identification and its differentiation from other Eucalyptus species.
Descritores: Terpenos
Fenômenos Biológicos
Óleos Voláteis
Myrtaceae
-Microscopia
Responsável: BR1.1 - BIREME


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Id: biblio-950753
Autor: Zeb, Anwar; Sadiq, Abdul; Ullah, Farhat; Ahmad, Sajjad; Ayaz, Muhammad.
Título: Phytochemical and toxicological investigations of crude methanolic extracts, subsequent fractions and crude saponins of Isodon rugosus
Fonte: Biol. Res;47:1-6, 2014. tab.
Idioma: en.
Resumo: BACKGROUND: Isodon rugosus is used traditionally in the management of hypertension, rheumatism, tooth-ache and pyrexia. Present study was arranged to investigate I. rugosus for phytoconstituents, phytotoxic and cytotoxic activities to explore its toxicological, pharmacological potentials and to rationalize its ethnomedicinal uses. Briefly, qualitative phytochemical analysis of the plant extracts were carried out for the existence of alkaloids, flavonoids, saponins, oils, glycosides, anthraquinones, terpenoids, sterols and tannins. Plant crude methanolic extract (Ir.Cr), its subsequent fractions; n-hexane (Ir.Hex), chloroform (Ir.Chf), ethyl acetate (Ir.EtAc), aqueous (Ir.Aq) and saponins (Ir.Sp) in different concentrations were tested for phytotoxic and cytotoxic activities using radish seeds and brine shrimps (Artemia salina) respectively. The phytotoxic activity was determined by percent root length inhibition (RLI) and percent seeds germination inhibition (SGI) while the cytotoxicity was obtained with percent lethality of the brine shrimps. RESULTS: Ir.Cr was tested positive for the presence of alkaloids, glycosides, flavonoids, oils, terpenoids, saponins, tannins and anthraquinones. Among different fractions Ir.Sp, Ir.Chf, Ir.EtAc, and Ir.Cr were most effective causing 93.55, 89.32, 81.32 and 58.68% inhibition of seeds in phytotoxicity assay, with IC50 values of 0.1, 0.1, 0.1 and 52 µg/ml respectively. Similarly, among all the tested samples, Ir.Sp exhibited the highest phytotoxic effect causing 91.33% root length inhibition with IC50 of 0.1 µg/ml. Ir.Sp and Ir.Chf were most effective against brine shrimps showing 92.23 and 76.67% lethality with LC50 values of 10 and 12 µg/ml respectively. CONCLUSIONS: It may be inferred from the current investigations that I. rugosus contains different secondary metabolites and is a potential source for the isolation of natural anticancer and herbicidal drug molecules. Different fractions exhibited phytotoxic and cytotoxic activities, thus providing pharmacological basis for ethnomedicinal uses of this plant.
Descritores: Saponinas/análise
Extratos Vegetais/farmacologia
Isodon/química
Fitoterapia
-Artemia/efeitos dos fármacos
Taninos/análise
Terpenos/análise
Flavonoides/análise
Óleos Vegetais/análise
Clorofórmio
Antraquinonas/análise
Raízes de Plantas/crescimento & desenvolvimento
Citotoxinas
Componentes Aéreos da Planta/química
Metanol
Alcaloides/análise
Glicosídeos/análise
Hexanos
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1003342
Autor: Ortega-Cuadros, Mailen; Tofiño-Rivera, Adriana Patricia; Merini, Luciano José; Martínez-Pabón, María Cecilia.
Título: Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effects
Fonte: Rev. biol. trop;66(4):1519-1529, oct.-dic. 2018. tab, graf.
Idioma: es.
Resumo: Resumen La caries dental, es una patología de origen multifactorial y actualmente los productos naturales son un tratamiento alternativo eficiente. El trabajo buscó evaluar la actividad antimicrobiana del aceite esencial de tres sustancias (Cymbopogon citratus y los componentes citral y mirceno) contra Streptococcus mutans ATCC UA159, así como su citotoxicidad sobre queratinocitos y fibroblastos humanos. A través de Microscopía Electrónica de Barrido, se observó la morfología típica de S. mutans, y una formación característica de polisacáridos, con una disposición más estructurada a las 48 h. El efecto de viabilidad bacteriana contra S. mutans en biofilms se evaluó a través de la exposición a las tres sustancias mediante el uso de la técnica de alto rendimiento MBEC, a concentraciones de 1, 0.1 y 0.01 μg/ml y clorhexidina como control positivo. La citotoxicidad de los compuestos se evaluó sobre queratinocitos y fibroblastos, mediante la técnica de reducción de MTT, utilizando 0.5 mM de H2O2 como control de muerte celular (control negativo) y etanol 1 % como control del vehículo (control positivo). Las tres sustancias evaluadas, tuvieron efectos sobre la viabilidad bacteriana de S. mutans con una mortalidad entre 74 y 96 %, sin diferencia significativa entre ellos (P > 0.393); adicionalmente, no fue evidente la citotoxicidad sobre queratinocitos y fibroblastos en un tratamiento de 24 h. Las sustancias evaluadas mostraron efectos antimicrobianos significativos; por lo tanto, estos deben estudiarse más a fondo como posibles coadyuvantes para prevenir la caries dental que causa efectos adversos menores.(AU)

Abstract Dental caries is a pathology of multifactorial origin and currently natural products are an efficient alternative treatment. The work sought to evaluate the antimicrobial activity of the three substances (Cymbopogon citratus essential oil and the citral and myrcene components) against Streptococcus mutans ATCC UA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. Through Scanning electron microscopy, the typical morphology of S. mutans was observed and, a characteristic formation of polysaccharides, with more structured arrangement at 48 h. The bacterial viability effect against S. mutans on biofilms was evaluated through exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1, 0.1, and 0.01 µg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated on keratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control (negative control) and ethanol 1 % as vehicle control (positive control). The three substances evaluated had effects on the bacterial viability of S. mutans with mortality between 74 and 96 %, without significant difference among them (P > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24 h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studied further as potential co-adjuvants to prevent dental caries that cause minor adverse effects.(AU)
Descritores: Terpenos
Óleos Voláteis
Biofilmes
Cárie Dentária/terapia
Fitoterapia/instrumentação
Responsável: BR1.1 - BIREME



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