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Masson, Maria Lucia
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Id: biblio-1055403
Autor: Ferreira, Bruna Mayara Roldão; Dagostin, João Luiz Andreotti; Andrade, Eriel Forville de; Takashina, Thiago Atsushi; Ellendersen, Luciana de Souza Neves; Masson, Maria Lucia.
Título: Relationship Between Parameters of Development and Functional Compounds of Yacon Leaves
Fonte: Braz. arch. biol. technol;62:e19180439, 2019. tab, graf.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Resumo: Abstract Yacon is a tuberous root from the Andean region being increasingly grown across the world due to the low caloric values of their roots and the functional properties of extractions of its leaves. However, there is still a gap in the knowledge of how the plant develops, and if physical and chemical changes are noticed regarding maturity. The subject of this work was to investigate the dynamics of a group of parameters and to establish correlations across them. To achieve this goal, leaves collected from three different parts of the plant in three months were assessed regarding size (plant height and leaf area), color and the presence of sesquiterpene lactones. Different methods of extraction were also studied and the total phenolic content was analyzed. The analysis of the results revealed different patterns in the quantity, size and leaf distribution according to the age of the plant. From three different methods of extraction studied, decoction seems to be the most appropriate one to obtain phenolic compounds with greater yield and safety. Besides, the greenness of yacon leaves showed a linear correlation to the total phenolic content, being a simple and promising estimator of the corresponding bioactive group. Sesquiterpene lactones were identified in all extracts, indicating the ease of their extractability even in simple procedures of tea-making.
Descritores: Raízes de Plantas
Fatores Físicos e Químicos
Compostos Fenólicos
Lactonas
Responsável: BR1.1 - BIREME


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Id: biblio-950821
Autor: Dzoyem, Jean P; Tsamo, Armelle T; Melong, Raduis; Mkounga, Pierre; Nkengfack, Augustin E; McGaw, Lyndy J; Eloff, Jacobus N.
Título: Cytotoxicity, nitric oxide and acetylcholinesterase inhibitory activity of three limonoids isolated from Trichilia welwitschii (Meliaceae)
Fonte: Biol. Res;48:1-6, 2015. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: Limonoids are highly oxygenated compounds with a prototypical structure. Their occurrence in the plant kingdom is mainly confined to plant families of Meliaceae and Rutaceae. Owing to their wide range of pharmacological and therapeutic properties, this study was aimed at investigating the potential nitric oxide (NO) and acetylcholinesterase (AChE) inhibitory activity and the cytotoxicity of three limonoids: trichilia lactone D5 (1), rohituka 3 (2) and dregeanin DM4 (3), isolated from Trichilia welwitschii C.DC. RESULTS: Results indicated that the three limonoids had low cytotoxicity towards Vero cells with LC50 values ranging from 89.17 to 75.82 µg/mL. Compounds (2) and (3) had lower cytotoxicity compared to puromycin and doxorubicin used as reference cytotoxic compounds. Compound (1) (LC50 of 23.55 µg/mL) had good antiproliferative activity against RAW 264.7 cancer cells. At the lowest concentration tested (0.5 µg/mL), compound (2) and (3) released the lowest amount of nitric oxide (2.97 and 2.93 µM, respectively). The three limonoids had anti-AChE activity with IC50 values ranged of 19.13 µg/mL for (1), 34.15 µg/mL for (2) and 45.66 µg/mL for (3), compared to galantamine (IC50 of 8.22 µg/mL) used as positive control. CONCLUSION: The limonoid compounds studied in this work inhibited nitric oxide production in LPS-stimulated macrophages and had anti-AChE activity. Trichilia lactone D5 had potential antiproliferative activity against RAW 264.7 cancer cells. The limonoids had low cytotoxicity towards Vero cells lines. This study provided further examples of the importance of limonoids compounds as potential AChE inhibitors and anti-inflammatory agents targeting the inhibition of NO production.
Descritores: Inibidores da Colinesterase/farmacologia
Meliaceae/química
Limoninas/farmacologia
Óxido Nítrico/antagonistas & inibidores
-Células Vero
Chlorocebus aethiops
Lipopolissacarídeos
Concentração Inibidora 50
Limoninas/isolamento & purificação
Limoninas/análise
Proliferação de Células/efeitos dos fármacos
Células RAW 264.7
Lactonas/análise
Lactonas/farmacologia
Dose Letal Mediana
Macrófagos/efeitos dos fármacos
Anti-Inflamatórios/farmacologia
Óxido Nítrico/análise
Limites: Animais
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1132268
Autor: Lino, Renata Calegari; Carvalho, Sabrina Matos de; Noronha, Carolina Montanheiro; Sganzerla, William Gustavo; Rosa, Cleonice Gonçalves da; Nunes, Michael Ramos; Barreto, Pedro Luiz Manique.
Título: Development and Characterization of Poly-ε -caprolactone Nanocapsules Containing β -carotene Using the Nanoprecipitation Method and Optimized by Response Surface Methodology
Fonte: Braz. arch. biol. technol;63:e20190184, 2020. tab, graf.
Idioma: en.
Resumo: Abstract Nanoparticles demonstrate an important role in the protection of bioactive compounds from external factors such as temperature, oxygen and light. In this study, poly-ε-caprolactone (PCL) nanoparticles entrapped β-carotene was produced using the nanoprecipitation method. Firstly, was evaluated the lipophilic surfactant effect and carrier agent of the active compound in the nanocapsules formulation. After choosing the most stable formulation, the nanocapsules production was optimized using β-carotene, caprylic/capric triglycerides (CCT) and soybean lecithin. Response surface methodology (RSM) was adopted to evaluate the influence of soy lecithin concentration, volume of CCT and β-carotene concentration in the particle size, zeta potential, polydispersity index (PDI), encapsulation efficiency and recovery. Formulations containing soy lecithin and CCT demonstrated better stability comparing to the other formulations tested. The nanoparticle formulations presented an optimized particle size below 200 nm, PDI lower than 0.1 and encapsulation efficiency above 95%. Based on the results obtained, the optimum conditions to prepare PCL nanocapsules were 0.2160 mg/mL of β-carotene, 232.42 μL of CCT and 2.59 mg/mL of soy lecithin, suggesting an applicability to promote controlled released of β-carotene in food system.
Descritores: Caproatos
beta Caroteno
Nanotecnologia/métodos
Nanocápsulas
Lactonas
-Precipitação Química
Reatores Biológicos
Otimização de Processos
Responsável: BR1.1 - BIREME


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Id: biblio-886616
Autor: ALMEIDA, MACIA C S DE; SOUZA, LUCIANA G S; FERREIRA, DANIELE A; PINTO, FRANCISCO C L; OLIVEIRA, DÉBORA R DE; SANTIAGO, GILVANDETE M P; MONTE, FRANCISCO J Q; BRAZ-FILHO, RAIMUNDO; LEMOS, TELMA L G DE.
Título: 7-epi-griffonilide, a new lactone from Bauhinia pentandra: complete 1H and 13C chemical shift assignments
Fonte: An. acad. bras. ciênc;89(1):65-71, Jan,-Mar. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT A new lactone, 7-epi-griffonilide (1), and six known compounds, 2, 3a - 3c, 4a and 4b, were isolated from the leaves of Bauhinia pentandra (Fabaceae). The structures elucidation of 1 and 2 were based on detailed 2D NMR techniques and spectral comparison with related compounds, leading to complete assignment of the 1H and 13C NMR spectra.
Descritores: Folhas de Planta/química
Bauhinia/química
Lactonas/isolamento & purificação
Lactonas/química
-Valores de Referência
Estereoisomerismo
Estrutura Molecular
Espectroscopia de Ressonância Magnética Nuclear de Carbono-13/métodos
Espectroscopia de Prótons por Ressonância Magnética/métodos
Responsável: BR1.1 - BIREME


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Zanella, Maria Teresa
Ribeiro, Artur Beltrame
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Id: lil-435164
Autor: Zanella, Maria Teresa; Uehara, Marcelo Hiroshi; Ribeiro, Artur Beltrame; Bertolami, Marcelo; Falsetti, Ana Claudia; Yunes, Mirela A.
Título: Orlistat and cardiovascular risk profile in hypertensive patients with metabolic syndrome: the ARCOS study / Avaliação do risco cardiovascular em pacientes obesos hipertensos com síndrome metabólica: estudo ARCOS
Fonte: Arq. bras. endocrinol. metab;50(2):368-376, abr. 2006. ilus, tab.
Idioma: en; pt.
Resumo: Weight loss improves metabolic abnormalities and reduces cardiovascular risk in obese hypertensive patients. To evaluate the impact of a sustained weight loss on coronary risk, 181 hypertensive patients with metabolic syndrome underwent to orlistat therapy, 120 mg, t.i.d., plus diet for 36 weeks. During therapy, Framingham risk scores (FRS) were calculated for determination of coronary heart disease risk in ten years. Body mass index decreased from 35.0 ± 4.2 to 32.6 ± 4.5 kg/m² (p< 0.0001) and waist circumference from 108.1 ± 10.1 to 100.5 ± 11.1 cm (p< 0.0001), at the end of the study period (week 36). Systolic and diastolic blood pressure showed reductions after the two first weeks, which were maintained up to the end of the study. A clear shift to the left in FRS distribution curve occurred at the end of the study, compared to baseline, indicating a reduction in coronary risk. Over all patients at risk, 49.2 percent moved to a lower risk category. A weight loss > 5 percent occurred in 64.6 percent of all patients, associated with improvement in glucose metabolism. Among those with abnormal glucose metabolism, 38 out 53 patients (71.7 percent) improved their glucose tolerance (p< 0.0005). In conclusion, long-term orlistat therapy helps to reduce and maintain a lower body weight, decreasing risk of coronary disease and improving glucose metabolism, thus protecting against type 2 diabetes.

A perda de peso melhora as anormalidades metabólicas e reduz o risco cardiovascular em pacientes obesos hipertensos. Com o objetivo de avaliar o impacto da perda de peso mantida sobre o risco coronariano, submetemos 181 pacientes hipertensos com síndrome metabólica à terapia com orlistat, 120 mg, três vezes ao dia, mais dieta, por um período de 36 semanas. Durante a terapia, foram calculados os scores de risco de Framingham (FRS) para a determinação do risco de doença cardíaca coronariana em dez anos. Ao final do período de estudo (semana 36), o índice de massa corpóreo diminuiu de 35,0 ± 4,2 para 32,6 ± 4,5 kg/m² (p< 0,0001) e a circunferência da cintura de 108,1 ± 10,1 para 100,5 ± 11,1 cm (p< 0,0001). As pressões sistólica e diastólica mostraram reduções após as primeiras duas semanas, que se mantiveram até o final do estudo. Um deslocamento evidente para a esquerda na curva de distribuição do FRS ocorreu no final do estudo, em comparação com os valores basais, indicando redução no risco coronariano. Do total de pacientes em risco, 49,2 por cento passou para uma categoria de risco menor. Ocorreu perda de peso > 5 por cento em 64,6 por cento de todos os pacientes, associada com melhora no metabolismo da glicose. Entre os 53 pacientes com metabolismo de glicose anormal, 38 (71,7 por cento) melhoraram sua tolerância à glicose (p< 0,0005). Em conclusão, terapia de longa duração com orlistat auxilia a reduzir e manter mais baixo o peso corpóreo, reduzindo o risco de doença coronária e melhorando o metabolismo da glicose e protegendo, dessa maneira, contra o diabetes tipo 2.
Descritores: Fármacos Antiobesidade/uso terapêutico
Lactonas/uso terapêutico
Obesidade/tratamento farmacológico
Perda de Peso/efeitos dos fármacos
-Fármacos Antiobesidade/farmacologia
Pressão Sanguínea
Índice de Massa Corporal
Seguimentos
Hipertensão/complicações
Lactonas/farmacologia
Síndrome Metabólica
Obesidade/dietoterapia
Relação Cintura-Quadril
Limites: Humanos
Masculino
Feminino
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


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Id: biblio-949352
Autor: Menezes-Rodrigues, Francisco Sandro; Errante, Paolo Ruggero; Ferreira, Regiane Miranda; Tavares, José Gustavo Padrão; Paula, Luciana de; Araújo, Erisvaldo Amarante; Govato, Tânia Carmem Peñaranda; Tikazawa, Eduardo Hiroshi; Reis, Maria do Carmo Maia; Luna-Filho, Bráulio; FerrazI, Renato Ribeiro Nogueira; Souza Oliveira-Júnior, Itamar de; Taha, Murched Omar; Caricati-Neto, Afonso.
Título: Cardioprotective effect of lipstatin derivative orlistat on normotensive rats submitted to cardiac ischemia and reperfusion
Fonte: Acta cir. bras;33(6):524-532, June 2018. graf.
Idioma: en.
Resumo: Abstract Purpose: To evaluate in vivo animal model of cardiac ischemia/reperfusion the cardioprotective activity of pancreatic lipase inhibitor of the orlistat. Methods: Adult male Wistar rats were anesthetized, placed on mechanical ventilation and underwent surgery to induce cardiac I/R by obstructing left descending coronary artery followed by reperfusion to evaluation of ventricular arrhythmias (VA), atrioventricular block (AVB) and lethality (LET) with pancreatic lipase inhibitor orlistat (ORL). At the end of reperfusion, blood samples were collected for determination of triglycerides (TG), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), high-density lipoprotein (HDL), lactate dehydrogenase (LDH), creatine kinase (CK), and creatine kinase-MB (CK-MB). Results: Treatment with ORL has been able to decrease the incidence of VA, AVB and LET. Besides that, treatment with ORL reduced serum concentrations of CK and LDL, but did not alter the levels of serum concentration of TG, VLDL and HDL. Conclusion: The reduction of ventricular arrhythmias, atrioventricular block, and lethality and serum levels of creatine kinase produced by treatment with orlistat in animal model of cardiac isquemia/reperfusion injury suggest that ORL could be used as an efficient cardioprotective therapeutic strategy to attenuate myocardial damage related to acute myocardial infarction.
Descritores: Cardiotônicos/farmacologia
Traumatismo por Reperfusão Miocárdica/prevenção & controle
Lactonas/farmacologia
Infarto do Miocárdio/prevenção & controle
-Arritmias Cardíacas/prevenção & controle
Triglicerídeos/sangue
Traumatismo por Reperfusão Miocárdica/sangue
Distribuição Aleatória
Reprodutibilidade dos Testes
Fatores de Risco
Resultado do Tratamento
Ratos Wistar
Creatina Quinase/sangue
Eletrocardiografia
Bloqueio Atrioventricular/prevenção & controle
FROSTBITE1
L-Lactato Desidrogenase/sangue
Lipoproteínas HDL/sangue
Lipoproteínas LDL/sangue
Lipoproteínas VLDL/sangue
Infarto do Miocárdio/sangue
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: lil-747176
Autor: Fiorati, Regina Celia; Elui, Valeria Meirelles Carril.
Título: Social determinants of health, inequality and social inclusion among people with disabilities / Determinantes sociais da saúde, iniquidades e inclusão social entre pessoas com deficiência / Determinantes sociales de la salud, iniquidades e inclusión social entre personas con deficiencia
Fonte: Rev. latinoam. enferm;23(2):329-336, Feb-Apr/2015. graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: OBJECTIVE: to analyze the socio-familial and community inclusion and social participation of people with disabilities, as well as their inclusion in occupations in daily life. METHOD: qualitative study with data collected through open interviews concerning the participants' life histories and systematic observation. The sample was composed of ten individuals with acquired or congenital disabilities living in the region covered by a Family Health Center. The social conception of disability was the theoretical framework used. Data were analyzed according to an interpretative reconstructive approach based on Habermas' Theory of Communicative Action. RESULTS: the results show that the socio-familial and community inclusion of the study participants is conditioned to the social determinants of health and present high levels of social inequality expressed by difficult access to PHC and rehabilitation services, work and income, education, culture, transportation and social participation. CONCLUSION: there is a need to develop community-centered care programs in cooperation with PHC services aiming to cope with poverty and improve social inclusion. .

OBJETIVO: analisar a inclusão sociofamiliar e comunitária e a participação social de pessoas com deficiência, bem como sua inserção em ocupações na vida cotidiana. MÉTODO: estudo qualitativo, com coleta de dados por meio de entrevistas abertas sobre história de vida e observação sistemática. A amostra foi composta por dez pessoas com deficiência, adquirida ou congênita, moradoras de região adstrita a um Núcleo de Saúde da Família. O referencial teórico foi a concepção social da deficiência. Os dados foram analisados segundo abordagem interpretativa reconstrutiva, fundamentada no referencial da Teoria da Ação Comunicativa de Habermas. RESULTADOS: os resultados evidenciaram que a inclusão sociofamiliar e comunitária dos sujeitos do estudo condiciona-se a determinantes sociais da saúde, apresentando índices de iniquidades sociais, expressos pela dificuldade de acesso a serviços de Atenção Primária à Saúde e de reabilitação, trabalho e renda, educação, cultura, transporte e participação social. CONCLUSÃO: conclui-se a necessidade da elaboração de programas de atenção centrados na comunidade, voltados ao enfrentamento da pobreza e à inclusão social, em articulação com serviços de Atenção Primaria à Saúde. .

OBJETIVO: analizar la inclusión social familiar y comunitaria, y la participación social de personas con deficiencia, así como su inserción en ocupaciones en la vida cotidiana. MÉTODO: estudio cualitativo, con recolección de datos por medio de entrevistas abiertas sobre historia de vida y por observación sistemática. La muestra estuvo compuesta por diez personas con deficiencia, adquirida o congénita, habitantes de una región adscrita a un Núcleo de Salud de la Familia. El referencial teórico fue la concepción social de la deficiencia. Los datos fueron analizados según abordaje interpretativo reconstructivo, fundamentado en el referencial de la Teoría de la Acción Comunicativa de Habermas. RESULTADOS: los resultados evidenciaron que la inclusión social familiar y comunitaria de los sujetos del estudio se condiciona a determinantes sociales de la salud, presentando índices de iniquidades sociales, expresados por la dificultad de acceso a servicios de Atención Primaria de la Salud y de rehabilitación, trabajo y renta, educación, cultura, transporte y participación social. CONCLUSIÓN: se concluye que existe la necesidad de elaborar programas de atención centrados en la comunidad, dirigidos al enfrentamiento de la pobreza y a la inclusión social, en articulación con servicios de Atención Primaria a la Salud. .
Descritores: Antineoplásicos/farmacologia
Proteínas de Choque Térmico HSP90/metabolismo
Lactonas/farmacologia
/tratamento farmacológico
NEUROFIBROMATOSIS TEMEFOS/tratamento farmacológico
Oximas/farmacologia
-Linhagem Celular Tumoral
Proliferação de Células
Proteínas de Choque Térmico HSP90/antagonistas & inibidores
Camundongos Nus
Camundongos Transgênicos
/metabolismo
NEUROFIBROMATOSIS TEMEFOS/metabolismo
Proteólise
Transcriptoma/efeitos dos fármacos
Ensaios Antitumorais Modelo de Xenoenxerto
Limites: Humanos
Animais
Feminino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-787548
Autor: KNOB, Carollinie Dias; SILVA, Milena; GASPAROTO, Thaís Helena; OLIVEIRA, Carine Ervolino; AMÔR, Nádia Ghinelli; ARAKAWA, Nilton Syogo; COSTA, Fernando Batista; CAMPANELLI, Ana Paula.
Título: Effects of budlein A on human neutrophils and lymphocytes
Fonte: J. appl. oral sci;24(3):271-277graf.
Idioma: en.
Projeto: São Paulo Research Foundation; . São Paulo Research Foundation; . São Paulo Research Foundation.
Resumo: ABSTRACT Sesquiterpene lactones (SLs) are the active constituents of a variety of medicinal plants used in traditional medicine for the treatment of inflammatory diseases and other ailments. Objective In this study, we evaluated whether budlein A modulates the activation of innate and adaptive immune cells such as neutrophils and lymphocytes. Material and Methods Our research group has investigated several plant species and several compounds have been isolated, identified, and their medical potential evaluated. Budlein A is a SL isolated from the species Aldama buddlejiformis and A. robusta (Asteraceae) and shows anti-inflammatory and anti-nociceptive activities. Advances in understanding how plant-derived substances modulate the activation of innate and adaptive immune cells have led to the development of new therapies for human diseases. Results Budlein A inhibited MPO activity, IL-6, CXCL8, IL-10, and IL-12 production and induces neutrophil apoptosis. In contrast, budlein A inhibited lymphocyte proliferation and IL-2, IL-10, TGF-β, and IFN-γ production, but it did not lead to cell death. Conclusions Collectively, our results indicate that budlein A shows distinct immunomodulatory effects on immune cells.
Descritores: Sesquiterpenos/farmacologia
Linfócitos T/efeitos dos fármacos
Lactonas/farmacologia
Anti-Inflamatórios/farmacologia
Neutrófilos/efeitos dos fármacos
-Ensaio de Imunoadsorção Enzimática
Fatores de Crescimento Transformadores/análise
Fatores de Crescimento Transformadores/efeitos dos fármacos
Células Cultivadas
Reprodutibilidade dos Testes
Análise de Variância
Interleucina-8/análise
Interleucina-8/efeitos dos fármacos
Interleucinas/análise
Apoptose/efeitos dos fármacos
Peroxidase/análise
Peroxidase/efeitos dos fármacos
Asteraceae/química
Proliferação de Células/efeitos dos fármacos
Citometria de Fluxo
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-954855
Autor: Bordon, Maria L. A. C; Laurenti, Márcia D; Ribeiro, Susan Pereira; Toyama, Marcos H; Toyama, Daniela de O; Passero, Luiz Felipe D.
Título: Effect of phospholipase A2 inhibitors during infection caused by Leishmania (Leishmania) amazonensis
Fonte: J. venom. anim. toxins incl. trop. dis;24:21, 2018. tab, graf, ilus.
Idioma: en.
Projeto: São Paulo Research Foundation (FAPESP); . HCFMUSP-LIM50 and CNPq-PIBIC; . CAPES; . CNPq/CAPES.
Resumo: Lipid metabolites play an important role in parasite differentiation and virulence. Studies have revealed that Leishmania sp. uses prostaglandins to evade innate barriers, thus enabling the parasites to survive inside immune cells. Despite the role of the enzyme Phospholipase A2 (PLA2) in prostaglandins production, few studies have investigated the role of parasite PLA2 during the interaction between L. (L.) amazonensis and the host (in vitro and in vivo) immune cells. Methods: In the present work, the leishmanicidal effect of PLA2 inhibitors, methyl arachidonyl fluorophosphonate (MAFP), bromoenol lactone (BEL) and aristolochic acid (AA) were investigated in vitro (promastigote and intracellular amastigote forms of L. (L.) amazonensis) and during in vivo infection using BALB/c mice. Results: The aforementioned inhibitors were deleterious to promastigote and amastigote forms of the L. (L.) amazonensis and were non-toxic to peritoneal macrophages from BALB/c mice. L. (L.) amazonensis-infected BALB/c mice treated with the inhibitor BEL presented decreased lesion size and skin parasitism; however, BEL treatment induced hepatotoxicity in BALB/c mice. Conclusions: Results presented herein suggested that PLA2 inhibitors altered L. (L.) amazonensis viability. In spite of liver toxicity, treatment with BEL was the most selective compound in vitro, as well in vivo, resulting in lower skin parasitism in the infected mice. These findings corroborate the role of PLA2 in parasite virulence and maintenance in vertebrate hosts, and suggest that molecules structurally related to BEL should be considered when planning compounds against Leishmania sp.(AU)
Descritores: Inibidores de Fosfolipase A2/farmacologia
Leishmania/efeitos dos fármacos
Leishmania/parasitologia
-Técnicas In Vitro
Macrófagos Peritoneais/efeitos dos fármacos
Lactonas/antagonistas & inibidores
Camundongos Endogâmicos BALB C
Limites: Animais
Masculino
Ratos
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Cerqueira, Mônica M. O. P
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Id: biblio-833981
Autor: Picinin, Lidia C. A; Toaldo, Isabela M; Hoff, Rodrigo B; Souza, Fernando N; Leite, Mônica O; Fonseca, Leorges M; Diniz, Soraia A; Silva, Marcos X; Cerqueira, Mônica M. O. P; Bordignon-Luiz, Marilde T.
Título: Survey of pyrethroid, macrocyclic lactone and antibacterial residues in bulk milk tank from Minas Gerais State, Brazil / Resíduos de piretróides, lactonas macrocíclicas e antimicrobianos em amostras de leite de tanque no estado de Minas Gerais
Fonte: Pesqui. vet. bras = Braz. j. vet. res;37(2):97-104, fev. 2017. tab.
Idioma: en.
Resumo: A survey of veterinary drug residues in bulk milk tank from Minas Gerais State, Brazil, was carried out through a broad scope analysis. Here, 132 raw milk samples were collected at 45 dairy farms in Minas Gerais from August 2009 to February 2010, and analyzed for 42 analytes, comprising pyrethroids, macrocyclic lactones and antibacterials, using liquid chromatography coupled with mass spectrometry in tandem mode and gas chromatography with electron capture detection. Within all milk samples, at least one veterinary drug residue was identified in 40 milk samples (30.30%) by confirmatory tests, whereas 16 samples (12.12%) showed the presence of at least two residues. With regard to the Brazilian maximum residue levels, 11 milk samples (8.33%) were non-compliant according to Brazilian Legislation. The veterinary drugs detected in the non-compliant milk samples include penicillin V (one sample), abamectin (one sample) and cypermethrin (nine samples). Furthermore, the antibacterial screening methods failed to identify most of the positive samples that were detected by confirmatory tests, leading to a large discrepancy between the screening and confirmatory antimicrobial tests. Thus, the present study indicated that the veterinary drugs residues still represents a great concern for the milk production chain.(AU)

Avaliou-se a presença de 42 analitos, incluindo piretróides, lactonas macrocíclicas e antimicrobianos em 132 amostras de leite de tanque proveniente de 45 propriedades leiteiras localizadas no Estado de Minas Gerais. Para tal, utilizou-se a cromatografia líquida acoplada a espectrofotometria de massas tandem e cromatografia gasosa com detector com captura de elétrons. Dentre todas as amostras de leite, 40 (30,30%) amostras de leite de tanque apresentaram a presença de pelo menos um analito, enquanto 16 amostras (12,12%) de leite demonstraram a presença de pelo menos dois analitos. Considerando os limites estabelecidos pela legislação brasileira, 11 amostras de leite (8,33%) seriam consideradas como não conforme. Ademais, os testes de triagem para detecção de antimicrobianos no leite não conseguiram identificar a maioria das amostras positivas nos testes confirmatórios, levando a grande discrepância entre estes testes. Desta forma, os resultados do presente estudo indicam que os períodos de descarte do leite, especialmente para piretróides, não foram plenamente respeitados por todos os produtores de leite. Além disto, uma discrepância entre os resultados dos testes confirmatórios e os testes de triagem foi observada.(AU)
Descritores: Anti-Infecciosos/análise
Resíduos de Drogas/análise
Lactonas/análise
Leite/química
Piretrinas/análise
-Anti-Helmínticos
Bovinos
Praguicidas
Drogas Veterinárias/análise
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice



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