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Id: lil-492878
Autor: Suke, S. G; Ahmed, R. S; Pathak, R; Tripathi, A. K; Banerjee, B. D.
Título: Attenuation of phosphamidon-induced oxidative stress and immune dysfunction in rats treated with N-acetylcysteine
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;41(9):765-768, Sept. 2008. tab.
Idioma: en.
Resumo: The effect of N-acetylcysteine, a thiolic antioxidant, on attenuation of phosphamidon-induced oxidative stress and immune dysfunction was evaluated in adult male Wistar rats weighing 200-250 g. Rats were divided into four groups, 8 animals/group, and treated with phosphamidon, N-acetylcysteine or the combination of both for 28 days. Oral administration of phosphamidon (1.74 mg/kg), an organophosphate insecticide, increased serum malondialdehyde (3.83 ± 0.18 vs 2.91 ± 0.24 nmol/mL; P < 0.05) and decreased erythrocyte superoxide dismutase (567.8 ± 24.36 vs 749.16 ± 102.61 U/gHb; P < 0.05), catalase activity (1.86 ± 0.18 vs 2.43 ± 0.08 U/gHb; P < 0.05) and whole blood glutathione levels (1.25 ± 0.21 vs 2.28 ± 0.08 mg/gHb; P < 0.05) showing phosphamidon-induced oxidative stress. Phosphamidon exposure markedly suppressed humoral immune response as assessed by antibody titer to ovalbumin (4.71 ± 0.51 vs 8.00 ± 0.12 -log2; P < 0.05), and cell-mediated immune response as assessed by leukocyte migration inhibition (25.24 ± 1.04 vs 70.8 ± 1.09%; P < 0.05) and macrophage migration inhibition (20.38 ± 0.99 vs 67.16 ± 5.30%; P < 0.05) response. Phosphamidon exposure decreased IFN-у levels (40.7 ± 3.21 vs 55.84 ± 3.02 pg/mL; P < 0.05) suggesting a profound effect of phosphamidon on cell-mediated immune response. A phosphamidon-induced increase in TNF-α level (64.19 ± 6.0 vs 23.16 ± 4.0 pg/mL; P < 0.05) suggests a contributory role of immunocytes in oxidative stress. Co-administration of N-acetylcysteine (3.5 mmol/kg, orally) with phosphamidon attenuated the adverse effects of phosphamidon. These findings suggest that oral N-acetylcysteine treatment exerts protective effect and attenuates free radical injury and immune dysfunction caused by subchronic phosphamidon exposure.
Descritores: Acetilcisteína/farmacologia
Formação de Anticorpos/efeitos dos fármacos
Depuradores de Radicais Livres/farmacologia
Inseticidas/toxicidade
Estresse Oxidativo/efeitos dos fármacos
Fosfamidona/toxicidade
-Formação de Anticorpos/imunologia
Ensaios de Migração de Leucócitos
Glutationa/sangue
Imunidade Celular/efeitos dos fármacos
Interferon gama/metabolismo
Malondialdeído/sangue
Ovalbumina/imunologia
Ratos Wistar
Superóxido Dismutase/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME



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