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Id: biblio-886879
Autor: CIPRIANO, JAMILE L D; CRUZ, ANA CLÁUDIA F; MANCINI, KARINA C; SCHMILDT, EDILSON R; LOPES, JOSÉ CARLOS; OTONI, WAGNER C; ALEXANDRE, RODRIGO S.
Título: Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects
Fonte: An. acad. bras. ciênc;90(1):385-400, Mar. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT The aim of this study was to evaluate somatic embryogenesis in juvenile explants of the THB papaya cultivar. Apical shoots and cotyledonary leaves were inoculated in an induction medium composed of different concentrations of 2,4-D (6, 9, 12, 15 and 18 µM) or 4-CPA (19, 22, 25, 28 and 31 µM). The embryogenic calluses were transferred to a maturation medium for 30 days. Histological analysis were done during the induction and scanning electron microscopy after maturing. For both types of auxin, embryogenesis was achieved at higher frequencies with cotyledonary leaves incubated in induction medium than with apical shoots; except for callogenesis. The early-stage embryos (e.g., globular or heart-shape) predominated. Among the auxins, best results were observed in cotyledonary leaves induced with 4-CPA (25 µM). Histological analyses of the cotyledonary leaf-derived calluses confirmed that the somatic embryos (SEs) formed from parenchyma cells, predominantly differentiated via indirect and multicellular origin and infrequently via synchronized embryogenesis. The secondary embryogenesis was observed during induction and maturation phases in papaya THB cultivar. The combination of ABA (0.5 µM) and AC (15 g L-1) in maturation medium resulted in the highest somatic embryogenesis induction frequency (70 SEs callus-1) and the lowest percentage of early germination (4%).
Descritores: Brotos de Planta/fisiologia
Carica/embriologia
Carica/fisiologia
Técnicas de Embriogênese Somática de Plantas/métodos
Ácidos Indolacéticos/análise
-Reguladores de Crescimento de Planta/farmacologia
Microscopia Eletrônica de Varredura
Ácido Abscísico/farmacologia
Brotos de Planta/efeitos dos fármacos
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/fisiologia
Germinação/efeitos dos fármacos
Germinação/fisiologia
Meios de Cultura
Carica/anatomia & histologia
Carica/efeitos dos fármacos
Responsável: BR1.1 - BIREME


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Id: biblio-974339
Autor: Ribeiro, Vitória Palhares; Marriel, Ivanildo Evódio; Sousa, Sylvia Morais de; Lana, Ubiraci Gomes de Paula; Mattos, Bianca Braz; Oliveira, Christiane Abreu de; Gomes, Eliane Aparecida.
Título: Endophytic Bacillus strains enhance pearl millet growth and nutrient uptake under low-P
Fonte: Braz. j. microbiol;49(supl.1):40-46, 2018. tab.
Idioma: en.
Projeto: Fapemig; . MPCPAgro; . Embrapa.
Resumo: Abstract Bacterial endophytes are considered to have a beneficial effect on host plants, improving their growth by different mechanisms. The objective of this study was to investigate the capacity of four endophytic Bacillus strains to solubilize iron phosphate (Fe-P), produce siderophores and indole-acetic acid (IAA) in vitro, and to evaluate their plant growth promotion ability in greenhouse conditions by inoculation into pearl millet cultivated in a P-deficient soils without P fertilization, with Araxá rock phosphate or soluble triple superphosphate. All strains solubilized Fe-P and three of them produced carboxylate-type siderophores and high levels of IAA in the presence of tryptophan. Positive effect of inoculation of some of these strains on shoot and root dry weight and the N P K content of plants cultivated in soil with no P fertilization might result from the synergistic combination of multiple plant growth promoting (PGP) traits. Specifically, while B1923 enhanced shoot and root dry weight and root N P content of plants cultivated with no P added, B2084 and B2088 strains showed positive performance on biomass production and accumulation of N P K in the shoot, indicating that they have higher potential to be microbial biofertilizer candidates for commercial applications in the absence of fertilization.
Descritores: Bacillus/metabolismo
Alimentos/metabolismo
Pennisetum/crescimento & desenvolvimento
Pennisetum/microbiologia
Endófitos/metabolismo
Ácidos Indolacéticos/metabolismo
-Fosfatos/análise
Fosfatos/metabolismo
Bacillus/genética
Sideróforos/metabolismo
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/metabolismo
Raízes de Plantas/microbiologia
Pennisetum/metabolismo
Endófitos/genética
Ferro/metabolismo
Responsável: BR1.1 - BIREME


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Azevedo, Joäo Lucio de
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Id: biblio-889203
Autor: Castro, Renata Assis; Dourado, Manuella Nóbrega; Almeida, Jaqueline Raquel de; Lacava, Paulo Teixeira; Nave, André; Melo, Itamar Soares de; Azevedo, João Lucio de; Quecine, Maria Carolina.
Título: Mangrove endophyte promotes reforestation tree (Acacia polyphylla) growth
Fonte: Braz. j. microbiol;49(1):59-66, Jan.-Mar. 2018. tab, graf.
Idioma: en.
Projeto: FAPESP; . CNPq.
Resumo: ABSTRACT Mangroves are ecosystems located in the transition zone between land and sea that serve as a potential source of biotechnological resources. Brazil's extensive coast contains one of the largest mangrove forests in the world (encompassing an area of 25,000 km2 along all the coast). Endophytic bacteria were isolated from the following three plant species: Rhizophora mangle, Laguncularia racemosa and Avicennia nitida. A large number of these isolates, 115 in total, were evaluated for their ability to fix nitrogen and solubilize phosphorous. Bacteria that tested positive for both of these tests were examined further to determine their level of indole acetic acid production. Two strains with high indole acetic acid production were selected for use as inoculants for reforestation trees, and then the growth of the plants was evaluated under field conditions. The bacterium Pseudomonas fluorescens (strain MCR1.10) had a low phosphorus solubilization index, while this index was higher in the other strain used, Enterobacter sp. (strain MCR1.48). We used the reforestation tree Acacia polyphylla. The results indicate that inoculation with the MCR1.48 endophyte increases Acacia polyphylla shoot dry mass, demonstrating that this strain effectively promotes the plant's growth and fitness, which can be used in the seedling production of this tree. Therefore, we successfully screened the biotechnological potential of endophyte isolates from mangrove, with a focus on plant growth promotion, and selected a strain able to provide limited nutrients and hormones for in plant growth.
Descritores: Bactérias/isolamento & purificação
Árvores/microbiologia
Acacia/microbiologia
Endófitos/isolamento & purificação
-Filogenia
Bactérias/classificação
Bactérias/genética
Bactérias/metabolismo
Árvores/crescimento & desenvolvimento
Brasil
Acacia/crescimento & desenvolvimento
Áreas Alagadas
Endófitos/classificação
Endófitos/genética
Endófitos/metabolismo
Ácidos Indolacéticos/metabolismo
Responsável: BR1.1 - BIREME


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Id: biblio-1011265
Autor: Oliveira, W. F; Lima, E. M; Gomes, D. I; Alves, K. S; Santos, P. M; Azevedo, G. S; Mezzomo, R.
Título: Agronomic performance of Marandu grass treated with plant growth biostimulants in the Amazon biome / Desempenho agronômico de capim-marandu tratado com bioestimulantes no bioma Amazônia
Fonte: Arq. bras. med. vet. zootec. (Online);71(2):603-612, mar.-abr. 2019. tab, graf.
Idioma: en.
Resumo: The aim was to evaluate the effect of different doses of two biostimulants on the productivity and canopy structure of Urochloa brizantha cv. Marandu grass in the establishment fase. The study was conducted in Universidade Federal Rural da Amazônia, Parauapebas, Pará, Brazil. One module of 35 plots of 25m² were established. A completely randomized experimental design was used, with seven treatments and five replicates. The treatments included a control, 0.5, 1, and 2kg/ha of biostimulant A (BIOST.A); and 0.25, 0.5, and 1L/ha of biostimulant B (BIOST.B). Three collections were performed. The data for application of the two biostimulants were analyzed separately, using the Dummy variable method and regression analysis. The application of 2kg/ha BIOST.A resulted in increases of 842kg/ha in the forage mass. The application of BIOST.B on Marandu grass resulted in a linear increase in stem mass. The application of 2kg/ha BIOST.A in the establishment of Marandu grass result in higher growth rates, forage accumulation, and stem proportion in the canopy.(AU)

Objetivou-se avaliar o efeito de diferentes doses de dois bioestimulantes sobre a produtividade e a estrutura do dossel do capim Urochloa brizantha cv. Marandu na fase de estabelecimento. O estudo foi realizado na Universidade Federal Rural da Amazônia, Parauapebas, Pará, Brasil. Um módulo de 35 parcelas de 25m² foi estabelecido. Utilizou-se delineamento experimental inteiramente ao acaso, composto de sete tratamentos e cinco repetições cada. Os tratamentos incluídos no controle foram: 0,5, 1 e 2kg/ha de bioestimulante A (BIOST.A); 0,25; 0,5 e 1L/ha de bioestimulante B (BIOST.B). Foram realizadas três coletas. Os dados para a aplicação dos dois bioestimulantes foram analisados separadamente, utilizando-se organização por método variável Dummy e análise de regressão. A aplicação de 2kg/ha BIOST.A resultou em incrementos de 842kg/ha na massa de forragem. A aplicação do BIOST.B na grama marandu resultou em aumento linear na produção de massa do caule. A aplicação de 2kg/ha BIOST.A no estabelecimento de erva de marandu resultou em maiores taxas de crescimento, acumulação de forragem e proporção de caule no dossel.(AU)
Descritores: Ecossistema Amazônico
Fertilizantes
Poaceae/crescimento & desenvolvimento
Ácidos Indolacéticos
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-966520
Autor: Khurshid, Sana; Zahid, Chand; Husnain, Shahida.
Título: Indoleacetic acid production and chromium reduction by cyanobacteria Synechocystis SP. P2A (Chroococcales) immobilized in alginate beads / Produção de ácido indolacético e redução do cromo por cianobactérias Synechocystis SP. P2A (Chroococcales) imobilizadas em esferas de alginato
Fonte: Biosci. j. (Online);33(6):1592-1600, nov./dec. 2017. tab, ilus, graf.
Idioma: en.
Resumo: A simple extrusion method was used to entrap Synechocystis sp.P2A in alginate beads. The viability, growth response and Indoleacetic acid (IAA) production at different pH were studied in alginate immobilized Synechocystis sp.P2A. 2.6% sodium alginate (w/v) and pH-7 was found to be optimum for growth of Synechocystis sp. P2A as well as IAA production (79µg/ml). To prepare effective formulation for plant inoculation, alginate beads were further modified by coating with chitosan or chitosan-polyethylene glycol. Effect of all formulations containing Synechocystis sp. P2A in free and immobilized form on growth of Triticumaestivum was evaluated. Soil inoculation of entrapped Synechocystis in alginate beads coated with chitosan resulted in 20% increase in root length and 14% increase in dry weight as compared to non-inoculated seedlings. Free and immobilized cyanobacteria were allowed to grow in BG11 medium supplemented with 100µg/ml K2CrO4 and chromium reduction was measured at variable pH. At pH 7 immobilized showed 5% more reduction than free form. The current study showed that alginate immobilized Synechocystis sp. P2A can accomplish viable functions including plant growth promoting hormone production and chromium reduction and therefore propose an efficient and convenient method for storage and use of cyanobacteria.

Um método de extrusão simples foi utilizado para aprisionar Synechocystis sp.P2A em esferas de alginato. A viabilidade, a resposta ao crescimento e a produção de ácido indolacético (IAA) a diferentes pH foram estudadas na Synechocystis sp.P2A imobilizada com alginato. Alginato de sódio a 2,6% (p/v) e pH-7 revelou-se ótimo para o crescimento de Synechocystis sp. P2A, bem como para a produção de IAA (79 µg/ml). Para preparar uma formulação eficaz para inoculação de plantas, as esferas de alginato foram adicionalmente modificadas por revestimento com quitosano ou quitosano-polietileno glicol. O efeito de todas as formulações contendo Synechocystis sp. P2A em forma livre e imobilizada no crescimento de Triticumaestivum foi avaliado. A inoculação no solo com Synechocystis aprisionado em esferas de alginato revestidas com quitosano resultou em um aumento de 20% no comprimento da raiz e aumento de 14% no peso seco em comparação com mudas não inoculadas. As cianobactérias livres e imobilizadas foram deixadas crescer em meio BG11 suplementado com 100 µg/ml de K2CrO4 e a redução do cromo foi medida a um pH variável. A um pH 7 a forma imobilizada apresentou 5% mais de redução do que a forma livre. O presente estudo mostrou que o alginato imobilizado de Synechocystis sp. P2A pode realizar funções viáveis, incluindo a produção de hormônio promotor do crescimento de plantas e redução de cromo e, portanto, propor um método eficiente e conveniente para armazenamento e uso de cianobactérias.
Descritores: Synechocystis
Alginatos
Ácidos Indolacéticos
Responsável: BR396.1 - Biblioteca Central


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Id: biblio-911148
Autor: Mero Jalca, Otto Francisco; Cuásquer Fuel, Elias; García Lucas, Lucy Maritza; Ramos Rodríguez, Marcos Pedro; Jiménez González, Alfredo.
Título: Efecto de reguladores de crecimiento tipo auxínico para la regeneración de tejido vegetal en Bursera graveolens / Effect of auxinic growth regulators for the regeneration of plant tissue in Bursera graveolens
Fonte: Revista Cubana de Ciencias Forestales;5(3):259-269, 2017. ilus, tab.
Idioma: es.
Resumo: Bursera graveolens (palo santo) es una especie nativa muy utilizada por sus propiedades medicinales. Esta especie crece a orillas de quebradas y en montañas bajas y medias de hasta 1 300 m. snm en las costas de Ecuador y Perú. Esta investigación tuvo el objetivo de evaluar el efecto de reguladores de crecimiento tipo auxínico para la regeneración de tejido vegetal en B. graveolens. Las muestras de la especie se recolectaron en el bosque seco tropical ubicado en la comuna Quimis, Cantón Jipijapa, provincia de Manabí. Se emplearon cuatro concentraciones de auxina: ácido indolbutírico (AIB) (2 000, 4 000, 6 000, 8 000 ppm) para la regeneración de estaquillas de la especie objeto de estudio. Después de la siembra se registró a diferentes intervalos de tiempo la producción de brotes, callos y raíces, así como la mortalidad. Se utilizó un diseño completamente al azar con cuatro repeticiones. El procesamiento estadístico consistió en un análisis de varianza (ANOVA) y la prueba de Tukey para probar las diferencias entre las medias de los tratamientos. El sustrato utilizado fue tierra del bosque seco, tierra de guaba y arena de río, en una proporción de 4:2:1. Los mejores resultados de producción de brotes y callos, así como menor mortalidad, se alcanzaron con el tratamiento T4 (AIB en dosis de 8 000 ppm). En estos ensayos, no hubo formación de raíces en ninguno de los tratamientos, pero sí presencia de callos a los 60 días después de la siembra, obteniéndose medias por estaquillas entre 5,00 (tratamiento 3) y 11,25 (tratamiento 4).
Descritores: Bursera
Ácidos Indolacéticos
-Equador
Plantas Medicinais
Tipo de Publ: Relatório Técnico
Responsável: BR1.1 - BIREME


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Id: lil-769669
Autor: Zhao, Longfei; Xu, Yajun; Lai, Xin-He; Shan, Changjuan; Deng, Zhenshan; Ji, Yuliang.
Título: Screening and characterization of endophytic Bacillus and Paenibacillus strains from medicinal plant Lonicera japonica for use as potential plant growth promoters
Fonte: Braz. j. microbiol;46(4):977-989, Oct.-Dec. 2015. tab, graf.
Idioma: en.
Projeto: National Science Foundation of China; . Henan Provincial Education Department of Science and Technology Research Key Research Project; . Ministry of Education of Henan Province.
Resumo: Abstract A total of 48 endophytic bacteria were isolated from surface-sterilized tissues of the medicinal plant Lonicera japonica, which is grown in eastern China; six strains were selected for further study based on their potential ability to promote plant growth in vitro (siderophore and indoleacetic acid production). The bacteria were characterized by phylogenetically analyzing their 16S rRNA gene similarity, by examining their effect on the mycelial development of pathogenic fungi, by testing their potential plant growth-promoting characteristics, and by measuring wheat growth parameters after inoculation. Results showed that the number of endophytic bacteria in L. japonica varied among different tissues, but it remained relatively stable in the same tissues from four different plantation locations. Among the three endophytic strains, strains 122 and 124 both had high siderophore production, with the latter showing the highest phosphate solubilization activity (45.6 mg/L) and aminocyclopropane-1-carboxylic acid deaminase activity (47.3 nmol/mg/h). Strain 170 had the highest indoleacetic acid (IAA) production (49.2 mg/L) and cellulase and pectinase activities. After inoculation, most of the six selected isolates showed a strong capacity to promote wheat growth. Compared with the controls, the increase in the shoot length, root length, fresh weight, dry weight, and chlorophyll content was most remarkable in wheat seedlings inoculated with strain 130. The positive correlation between enzyme (cellulose and pectinase) activity and inhibition rate on Fusarium oxysporum, the IAA production, and the root length of wheat seedlings inoculated with each tested endophytic strain was significant in regression analysis. Deformity of pathogenic fungal mycelia was observed under a microscope after the interaction with the endophytic isolates. Such deformity may be directly related to the production of hydrolytic bacterial enzymes (cellulose and pectinase). The six endophytic bacterial strains were identified to be Paenibacillus and Bacillus strains based on the results of 16S rRNA gene sequencing analysis and their physiological and biochemical characteristics. Results indicate the promising application of endophytic bacteria to the biological control of pathogenic fungi and the improvement of wheat crop growth.
Descritores: Bacillus/classificação
Bacillus/genética
Bacillus/crescimento & desenvolvimento
Bacillus/isolamento & purificação
Bacillus/metabolismo
Bacillus/microbiologia
China/classificação
China/genética
China/crescimento & desenvolvimento
China/isolamento & purificação
China/metabolismo
China/microbiologia
Endófitos/classificação
Endófitos/genética
Endófitos/crescimento & desenvolvimento
Endófitos/isolamento & purificação
Endófitos/metabolismo
Endófitos/microbiologia
Ácidos Indolacéticos/classificação
Ácidos Indolacéticos/genética
Ácidos Indolacéticos/crescimento & desenvolvimento
Ácidos Indolacéticos/isolamento & purificação
Ácidos Indolacéticos/metabolismo
Ácidos Indolacéticos/microbiologia
Lonicera/classificação
Lonicera/genética
Lonicera/crescimento & desenvolvimento
Lonicera/isolamento & purificação
Lonicera/metabolismo
Lonicera/microbiologia
Dados de Sequência Molecular/classificação
Dados de Sequência Molecular/genética
Dados de Sequência Molecular/crescimento & desenvolvimento
Dados de Sequência Molecular/isolamento & purificação
Dados de Sequência Molecular/metabolismo
Dados de Sequência Molecular/microbiologia
Paenibacillus/classificação
Paenibacillus/genética
Paenibacillus/crescimento & desenvolvimento
Paenibacillus/isolamento & purificação
Paenibacillus/metabolismo
Paenibacillus/microbiologia
Filogenia/classificação
Filogenia/genética
Filogenia/crescimento & desenvolvimento
Filogenia/isolamento & purificação
Filogenia/metabolismo
Filogenia/microbiologia
Raízes de Plantas/classificação
Raízes de Plantas/genética
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/isolamento & purificação
Raízes de Plantas/metabolismo
Raízes de Plantas/microbiologia
Sideróforos/classificação
Sideróforos/genética
Sideróforos/crescimento & desenvolvimento
Sideróforos/isolamento & purificação
Sideróforos/metabolismo
Sideróforos/microbiologia
Triticum/classificação
Triticum/genética
Triticum/crescimento & desenvolvimento
Triticum/isolamento & purificação
Triticum/metabolismo
Triticum/microbiologia
Responsável: BR1.1 - BIREME


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Id: lil-753687
Autor: Ahmed, Mehboob; Stal, Lucas J.; Hasnain, Shahida.
Título: The morphology and bioactivity of the rice field cyanobacterium Leptolyngbya / Morfología y bioactividad de la cianobacteria Leptolyngbya en los campos de cultivo de arroz
Fonte: Rev. biol. trop;62(3):1251-1260, jul.-sep. 2014. ilus, graf, tab.
Idioma: en.
Resumo: The genus Leptolyngbya comprises filamentous cyanobacteria that are important in rice fields. In the rhizosphere, cyanobacteria produce a variety of secondary metabolites such as auxins that are important in agriculture soil performance. To assess this, Leptolyngbya strain MMG-1, was isolated from the rhizosphere of rice plants and described. For this, the morphology of this strain was studied by light microscopy as well as by confocal laser scanning microscopy. Besides, the ability of this strain to synthesize an auxin-like bioactive compound was demonstrated under various culture conditions (different amounts of tryptophan; pH; different alternating light:dark periods; duration of the incubation). The auxin-like compound was extracted from the culture of Leptolyngbya strain MMG-1 and identified as indole-3-acetic acid (IAA) by thin layer chromatography (TLC) as well as by high performance liquid chromatography (HPLC). Our results showed that the strain required the precursor L-tryptophan for the synthesis of IAA. Leptolyngbya strain MMG-1 accumulated IAA intracellularly. The IAA secreted by Leptolyngbya strain MMG-1 was significantly correlated with the initial concentration of L-tryptophan in the medium, as well as with the duration of the incubation. The bioactivity of the secreted IAA was determined by its effect on the rooting pattern of Pisum sativum seedlings. The culture supernatant of Leptolyngbya strain MMG-1 stimulated the seedling lateral rooting, while it decreased root length. Hence, rhizospheric Leptolyngbya produced auxin under different conditions and affected the plants rooting pattern. Rev. Biol. Trop. 62 (3): 1251-1260. Epub 2014 September 01.

El género Leptolyngbya comprende cianobacterias filamentosas que son importantes en los campos de cultivo de arroz. En la rizosfera, las cianobacterias producen una variedad de metabolitos secundarios, tales como auxinas, que son importantes en el rendimiento de la agricultura del suelo. La cepa Leptolyngbya MMG-1, fue aislada de la rizosfera de plantas de arroz y se describe en este trabajo. La morfología de esta cepa se estudió por microscopía de luz, así como por microscopía confocal de barrido láser. Además, se estimó la capacidad de esta cepa para sintetizar el compuesto bioactivo auxina como se demostró en diversas condiciones de cultivo (diferentes cantidades de triptófano; pH; diferente luz alterna: períodos oscuros; duración de la incubación). La auxina se extrajo a partir del cultivo de la cepa Leptolyngbya MMG-1 y se identificó como ácido indol-3-acético (AA) por cromatografía de capa fina (TLC), así como por cromatografía líquida de alta resolución (HPLC). Nuestros resultados mostraron que la cepa requiere el precursor de L-triptófano para la síntesis de IAA. La cepa Leptolyngbya MMG-1 acumula intracelularmente IAA. El IAA secretada por la cepa Leptolyngbya MMG-1 se correlacionó significativamente con la concentración inicial de L-triptófano en el medio, así como con la duración de la incubación. La bioactividad de la IAA secretada se determinó por su efecto sobre el patrón de enraizamiento de plantas de semillero de Pisum sativum. El sobrenadante del cultivo de la cepa Leptolyngbya MMG-1 estimuló el enraizamiento lateral en la plántula, mientras que se redujo la longitud de la raíz. Por lo tanto, la producción de auxina por Leptolyngbya rizosférica afectó el crecimiento de las plantas.
Descritores: Cianobactérias/fisiologia
Ácidos Indolacéticos/isolamento & purificação
Oryza/microbiologia
-Cianobactérias/química
Cianobactérias/classificação
Cianobactérias/isolamento & purificação
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CR1.1 - BINASSS - Biblioteca Nacional de Salud y Seguridad Social


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Id: lil-741272
Autor: Peng, Yanjie; He, Yanhui; Wu, Zhansheng; Lu, Jianjiang; Li, Chun.
Título: Screening and optimization of low-cost medium for Pseudomonas putida Rs-198 culture using RSM
Fonte: Braz. j. microbiol;45(4):1229-1237, Oct.-Dec. 2014. graf, tab.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Science and Technology Fund Projects of Shihezi University.
Resumo: The plant growth-promoting rhizobacterial strain Pseudomonas putida Rs-198 was isolated from salinized soils from Xinjiang Province. We optimized the composition of the low-cost medium of P. putida Rs-198 based on its bacterial concentration, as well as its phosphate-dissolving and indole acetic acid (IAA)-producing capabilities using the response surface methodology (RSM), and a mathematical model was developed to show the effect of each medium component and its interactions on phosphate dissolution and IAA production. The model predicted a maximum phosphate concentration in medium containing 63.23 mg/L inorganic phosphate with 49.22 g/L corn flour, 14.63 g/L soybean meal, 2.03 g/L K2HPO4, 0.19 g/L MnSO4 and 5.00 g/L NaCl. The maximum IAA concentration (18.73 mg/L) was predicted in medium containing 52.41 g/L corn flour, 15.82 g/L soybean meal, 2.40 g/L K2HPO4, 0.17 g/L MnSO4 and 5.00 g/L NaCl. These predicted values were also verified through experiments, with a cell density of 10(13) cfu/mL, phosphate dissolution of 64.33 mg/L, and IAA concentration of 18.08 mg/L. The excellent correlation between predicted and measured values of each model justifies the validity of both the response models. The study aims to provide a basis for industrialized fermentation using P. putida Rs-198.
Descritores: Meios de Cultura/química
Pseudomonas putida/crescimento & desenvolvimento
-Carga Bacteriana
Custos e Análise de Custo
Meios de Cultura/economia
Ácidos Indolacéticos/metabolismo
Programas de Rastreamento
Modelos Teóricos
Fosfatos/metabolismo
Pseudomonas putida/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-733139
Autor: Rodrigues, Ana Paula dos Santos; Silveira, Erika Aparecida da.
Título: Correlação e associação de renda e escolaridade com condições de saúde e nutrição em obesos graves / Correlation and association of income and educational level with health and nutritional conditions among the morbidly obese
Fonte: Ciênc. saúde coletiva;20(1):165-174, 01/2015. tab.
Idioma: pt.
Resumo: O objetivo deste artigo é investigar relações entre renda e escolaridade com condições de saúde e nutrição em obesos graves. Estudo transversal ambulatorial com 79 pacientes de primeira consulta, com Índice de Massa Corporal (IMC) ≥ 35 kg/m2 e idade ≥ 20 anos. Coletaram-se dados: sociodemográficos, antropométricos, estilo de vida, exames bioquímicos e consumo alimentar. O IMC médio foi 48,3 ± 6,9 kg/m2. Observou-se correlação negativa significante de escolaridade com variáveis peso (r = -0,234) e IMC (r = -0,364) e de renda familiar per capita com consumo diário de vegetal A (r = -0,263). Após análise multivariada maior renda familiar per capita se associou à ausência de cardiopatia (RP: 0,51, IC95%: 0,32-0,81), maior consumo diário de vegetal A (RP: 1,79, IC95%: 1,16-2,75) e doces (RP: 3,12, IC95%: 1,21-8,04). Em obesos graves a maior renda familiar per capita se associou à ausência de cardiopatia e maior consumo de vegetais folhosos e doces. Já a escolaridade não se manteve associada às condições de saúde e nutrição.

This article seeks to investigate the relationship between income and educational level and health and nutritional conditions among the morbidly obese. A cross-sectional study was conducted with 79 patients at first appointment, with Body Mass Index (BMI) ≥ 35 kg/m2 and age ≥ 20 years. The following data was collected: demographic, socioeconomic, anthropometric, lifestyle, biochemical and food intake data. Average BMI was 48.3 ± 6.9 kg/m2. There was a significant negative correlation between education level and the variables of weight (r = -0.234) and BMI (r = -0.364) and per capita family income with daily consumption of leafy vegetables (r = -0.263). After multivariate analysis, higher per capita family income was associated with the absence of heart disease (PR: 0.51, CI95%: 0.32-0.81), higher daily consumption of leafy vegetables (PR: 1.79, CI95%: 1.16-2.75) and candy (PR: 3.12, CI95%: 1.21-8.04). In the morbidly obese, per capita household income was associated with absence of heart disease and higher consumption of leafy vegetables and candy. On the other hand, education level was not associated with health and nutrition conditions.
Descritores: Arabidopsis/enzimologia
Arabidopsis/genética
Regulação da Expressão Gênica de Plantas
Ácidos Indolacéticos/metabolismo
Fosfolipases A/metabolismo
-/farmacologia
ABDOMEN,ABDOMINAL NEOPLASMS,ABELSON MURINE LEUKEMIA VIRUS,CONGENITAL ABNORMALITIES-EICOSATETRAYNOIC ACID/farmacologia
Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Proteínas de Ligação a DNA/genética
Proteínas de Ligação a DNA/metabolismo
Inibidores Enzimáticos/farmacologia
Glucuronidase/metabolismo
Luciferases/metabolismo
Proteínas Nucleares/genética
Proteínas Nucleares/metabolismo
Fosfolipases A/antagonistas & inibidores
Processamento de Proteína Pós-Traducional/efeitos dos fármacos
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Plântula/efeitos dos fármacos
Plântula/metabolismo
Fatores de Tempo
/farmacologia
TEMEFOS,ABBREVIATIONS AS TOPIC-DICHLOROPHENOXYACETIC ACID/farmacologia
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Responsável: BR1.1 - BIREME



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