Base de dados : LILACS
Pesquisa : D03. [Categoria DeCS]
Referências encontradas : 6 [refinar]
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Texto completo SciELO Chile
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Id: biblio-950895
Autor: Zhu, Liping; Han, Jiakai; Yuan, Rongrong; Xue, Lei; Pang, Wuyan.
Título: Berberine ameliorates diabetic nephropathy by inhibiting TLR4/NF-κB pathway
Fonte: Biol. Res;51:9, 2018. graf.
Idioma: en.
Resumo: BACKGROUND: Diabetic nephropathy (DN) is the leading cause of end-stage renal failure, contributing to severe morbidity and mortality in diabetic patients. Berberine (BBR) has been well characterized to exert renoprotective effects in DN progression. However, the action mechanism of BBR in DN remains to be fully understood. METHODS: The DN rat model was generated by intraperitoneal injection of streptozotocin (STZ, 65 mg/kg body weight) while 30 mM high glucose (HG)-treated podocytes were used as an in vitro DN model. The fasting blood glucose level and ratio of kidney weight to body weight were measured after BBR treatment (50, 100, or 200 mg/kg) in STZ-induced DN rats. The renal injury parameters including 24-h urinary protein, blood urea nitrogen and serum creatinine were assessed. qRT-PCR was performed to detect the transcript amounts of inflammatory factors. The concentrations of inflammatory factors were evaluated by ELISA kits. Western blot analysis was conducted to measure the amounts of TLR4/NF-κB-related proteins. The apoptotic rate of podocytes was analyzed by flow cytometry using Annexin V/propidium iodide. RESULTS: Berberine reduced renal injury in STZ-induced DN rat model, as evidenced by the decrease in fasting blood glucose, ratio of kidney weight to body weight, 24-h urinary protein, serum creatinine, and blood urine nitrogen. BBR attenuated the systemic and renal cortex inflammatory response and inhibited TLR4/NF-κB pathway in STZ-induced DN rats and HG-induced podocytes. Also, HG-induced apoptosis of podocytes was lowered by BBR administration. Furthermore, blockade of TLR4/NF-κB pathway by resatorvid (TAK-242) or pyrrolidine dithiocarbamate aggravated the inhibitory effect of BBR on HG-induced inflammatory response and apoptosis in podocytes. CONCLUSIONS: Berberine ameliorated DN through relieving STZ-induced renal injury, inflammatory response, and podocyte HG-induced apoptosis via inactivating TLR4/NF-κB pathway.
Descritores: Berberina/farmacologia
Transdução de Sinais/efeitos dos fármacos
NF-kappa B/antagonistas & inibidores
Diabetes Mellitus Experimental/tratamento farmacológico
Nefropatias Diabéticas/tratamento farmacológico
Receptor 4 Toll-Like/antagonistas & inibidores
-Ratos Sprague-Dawley
Limites: Animais
Responsável: CL1.1 - Biblioteca Central

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Id: biblio-971926
Autor: Silva, Anderson Ramos da.
Título: Avaliação in vitro da berberina frente às cepas de Candida spp. e Cryptococcus neoformansresistentes ao fluconazol e sua atividade em isolados formadores de biofilme.
Fonte: Fortaleza; s.n; 2016. 67 p. ilus.
Idioma: pt.
Tese: Apresentada a Universidade Federal do Ceará para obtenção do grau de Mestre.
Resumo: Atualmente, a incidência de infecções fúngicas tem aumentado significativamente,contribuindo assim para morbidade e mortalidade. Esse fato que tem como umas das principais causas o aumento da resistência antimicrobiana, estando associado à produção de biofilme que tem um elevado nível de resistência antimicrobiana juntamente com o arsenal farmacológico antifúngico limitado, quando comparado ao arsenal antibacteriano, leva a necessidade do desenvolvimento de novas estratégias terapêuticas. A berberina tem demonstrado ter ampla atividade antibacteriana e antifúngica. Dentro desse contexto, o objetivo foi avaliar o potencial antifúngico da berberina frente às cepas de Candida spp. e Cryptococcus neo formans resistentes ao fluconazol, bem como avaliar o efeito da berberina frente às cepas de Candida spp...

Currently, the incidence of fungal infections has increased significantly, contributing tomorbidity and mortality. It has as one of its main causes the increase of antibiotic resistance.Recently associated with biofilm production, which has a high level of antibiotic resistance,coupled with the limited antifungal pharmacological when compared to antibacterial, it leadsto the need for the development of new therapeutic strategies. The berberine has shown tohave broad antibacterial and antifungal activity. In this context, the objective was to evaluatethe antifungal potential of berberine against strains of Candida spp. and Cryptococcusneoformans resistant to fluconazole and evaluate the effect of berberine against strains ofCandida tropicalis in biofilm formation. The evaluation of the antifungal effect wasdetermined by microdilution in broth (M27-A3) and by flow cytometry techniques, in whichthe likely mechanism of action of that compound was evaluated. For the assessment of thebiofilm, a colorimetric assay (MTT) was used to determine the susceptibility of sessile cells.Strains of Candida spp...
Descritores: Candida
Cryptococcus neoformans
Limites: Humanos
Responsável: BR6.1 - BCS - Biblioteca de Ciências da Saúde

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Texto completo SciELO Chile
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Id: lil-710931
Autor: Dkhi, Mohamed A.
Título: Role of berberine in ameliorating Schistosoma mansoni-induced hepatic injury in mice
Fonte: Biol. Res;47:1-7, 2014. ilus, graf, tab.
Idioma: en.
Projeto: King Saud University. Deanship of Scientific Research.
Resumo: BACKGROUND: Schistosomiasis is caused by helminth parasites of the genus Schistosoma. Berberine chloride (BER), an isoquinoline alkaloid, has been used in vivo for its antiparasitic, antioxidant and hepatoprotective properties. In this study, the protective effect of BER and praziquantel has been compared for the extent of schistosomiasis-induced oxidative stress in hepatic tissue of mice. RESULTS: S. mansoni was able to induce inflammation and injury to the liver, evidenced (i) by an increase in inflammatory cellular infiltrations, dilated sinusoids and vacuolated hepatocytes, (ii) by decreased levels of alanine and aspartate aminotransferases and increased levels of alkaline phosphatase, γ-glutamyl transferase in the liver homogenate, (iii) by increased production of nitric oxide and thiobarbituric acid reactive substances, and (iv) by lowered glutathione levels and decreased activities of catalase and superoxide dismutase, respectively. All these infection-induced parameters were significantly altered during BER treatment. In particular, berberine counteracted the S. mansoni-induced loss of glutathione and the activities of catalase and superoxide dismutase. CONCLUSION: Based on these results, it is concluded that berberine could ameliorate pre-existing liver damage and oxidative stress conditions due to schistosomiasis.
Descritores: Berberina/uso terapêutico
Hepatopatias Parasitárias/tratamento farmacológico
Estresse Oxidativo/efeitos dos fármacos
Esquistossomose/tratamento farmacológico
-Alanina Transaminase/análise
Fosfatase Alcalina/análise
Aspartato Aminotransferases/análise
Infiltração de Neutrófilos
Óxido Nítrico/análise
Schistosoma mansoni
Superóxido Dismutase/metabolismo
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central

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Id: lil-694711
Autor: XU, Li-na; LU, Bi-nan; HU, Ming-ming; XU, You-wei; HAN, Xu; QI, Yan; PENG, Jin-yong.
Título: Mechanisms involved in the cytotoxic effects of berberine on human colon cancer HCT-8 cells
Fonte: Biocell;36(3):113-120, Dec. 2012. graf.
Idioma: en.
Projeto: Excellent Young Scientist funds; . Science and Technology Foundation of Dalian; . First Special China Post-Doctor Foundation; . Ministry of Education. Academic Scholarship for Doctoral Candidates.
Resumo: Berberine, a constituent of some traditional Chinese medicinal plants, has been reported to have cytotoxicity effects on different human cancer cell lines. There is no available information about the effects and mechanism of action of berberine on human colon cancer cell line HCT-8. In this paper, the cytotoxicity of berberine on HCT-8 cancer cells was investigated by MTT assay, fluorescence microscopy and flow cytometry analysis. Our data revealed that berberine could significantly inhibit the growth of HCT-8 cells in a dose- and time-dependent manner. Morphology of apoptotic cells was studied with acridine orange/ethidium bromide staining. The concentrations of lactate dehydrogenase and both acid and alkaline phosphatases were significantly increased in cell supernatants after berberine treatment, suggesting cell death. Furthermore, flow cytometry analysis showed that berberine could arrest HCT-8 cells at S phase in a time-dependent manner. To further investigate the apoptotic molecular mechanism, reverse transcription-polymerase chain reaction (RT-PCR) and western blotting methods were used. The up-regulated mRNA and/or protein expressions of Fas, FasL, TNF-a, caspase-3 and down-regulation of pro-caspase-3 suggest that the death receptor pathway may be involved in the apoptotic pathway induced by berberine. Decrease of Bcl-2 and increase of Bax in mRNA and/or protein expressions showed that the Bcl-2 family proteins were involved in berberine-induced apoptosis. We also found that berberine-induced apoptosis was associated with an up-regulated expressions of p53 and prohibitin (PHB), and decreased vimentin expression. These results suggest that berberine can suppress cell growth and reduce cell survival by arresting the cell-cycle and by inducing apoptosis of HCT-8 cells.
Descritores: Berberina/farmacologia
Neoplasias do Colo/tratamento farmacológico
Neoplasias do Colo/metabolismo
Ciclo Celular
Linhagem Celular Tumoral
Citometria de Fluxo
L-Lactato Desidrogenase/metabolismo
Medicina Tradicional Chinesa
Microscopia de Fluorescência
RNA Mensageiro/metabolismo
Proteínas Repressoras/farmacologia
Fase S
Fatores de Tempo
Sais de Tetrazólio/farmacologia
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas

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Id: lil-149867
Autor: Payo, Armando; Oquendo, Marledis; Sandoval, Daysi.
Título: Mahonia tenuifolia (Lindl.) Lour. Ex Fedde: una posible fuente de berberina en Cuba / Mahnia tenuifolian (Lindl.) Lour. Ex Fedde: a possible source of berberines in Cuba
Fonte: Rev. cuba. farm;27(2):134-8, jul.-dic. 1993. ilus.
Idioma: es.
Resumo: Mahonia tenuifolia (Lindl.) Lour. ex Fedde (Berberis tenuifolia Lindl.) es una planta perteneciente a la familia Berberidaceae que crece en las regiones occidental y central de Cuba. El presente trabajo informa sobre la presencia de berberina en las partes aéreas de esta especie y el contenido de este alcaloide en los tallos
Descritores: Alcaloides
Berberina/isolamento & purificação
Berberis aquifolium/análise
-Cromatografia em Camada Delgada
Responsável: CU1 - INFOMED - Centro Nacional de Información de Ciencias Médicas

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Id: lil-109889
Autor: Lazo, Waldo.
Título: Antimicrobianos producidos por plantas / Antimicrobial agents produced by plants
Fonte: Bol. micol;6(1/2):37-9, jul.-dic. 1991.
Idioma: es.
Resumo: Los principales antimicrobianos producidos por plantas son: ácido sórbico producido por Sorbus aucuparia L.; berberina por Berberis vulgaris L.; aliicina por Allium sativum L.; isotiocianato de bencilo por Tropaeolum majus L.; timol por Thymus vulgaris L. Se menciona también muchos otros antimicrobianos de menor importancia producidos por plantas
Descritores: Anti-Infecciosos
Plantas Medicinais
-Ácido Sórbico/antagonistas & inibidores
Antifúngicos/uso terapêutico
Berberina/uso terapêutico
Conservação de Alimentos
Timol/uso terapêutico
Responsável: CL2.1 - Biblioteca de Medicina

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