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Pesquisa : D03.383.533 [Categoria DeCS]
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Id: lil-780820
Autor: Ramya, Shanivarsanthe Leelesh; Venkatesan, Thiruvengadam; Srinivasa Murthy, Kottilingam; Jalali, Sushil Kumar; Verghese, Abraham.
Título: Detection of carboxylesterase and esterase activity in culturable gut bacterial flora isolated from diamondback moth, Plutella xylostella (Linnaeus), from India and its possible role in indoxacarb degradation
Fonte: Braz. j. microbiol;47(2):327-336, Apr.-June 2016. tab, graf.
Idioma: en.
Resumo: Abstract Diamondback moth (DBM), Plutella xylostella (Linnaeus), is a notorious pest of brassica crops worldwide and is resistant to all groups of insecticides. The insect system harbors diverse groups of microbiota, which in turn helps in enzymatic degradation of xenobiotic-like insecticides. The present study aimed to determine the diversity of gut microflora in DBM, quantify esterase activity and elucidate their possible role in degradation of indoxacarb. We screened 11 geographic populations of DBM in India and analyzed them for bacterial diversity. The culturable gut bacterial flora underwent molecular characterization with 16S rRNA. We obtained 25 bacterial isolates from larvae (n = 13) and adults (n = 12) of DBM. In larval gut isolates, gammaproteobacteria was the most abundant (76%), followed by bacilli (15.4%). Molecular characterization placed adult gut bacterial strains into three major classes based on abundance: gammaproteobacteria (66%), bacilli (16.7%) and flavobacteria (16.7%). Esterase activity from 19 gut bacterial isolates ranged from 0.072 to 2.32 µmol/min/mg protein. Esterase bands were observed in 15 bacterial strains and the banding pattern differed in Bacillus cereus – KC985225 and Pantoea agglomerans – KC985229. The bands were characterized as carboxylesterase with profenofos used as an inhibitor. Minimal media study showed that B. cereus degraded indoxacarb up to 20%, so it could use indoxacarb for metabolism and growth. Furthermore, esterase activity was greater with minimal media than control media: 1.87 versus 0.26 µmol/min/mg protein. Apart from the insect esterases, bacterial carboxylesterase may aid in the degradation of insecticides in DBM.
Descritores: Oxazinas/metabolismo
Bactérias/enzimologia
Carboxilesterase/metabolismo
Esterases/metabolismo
Microbioma Gastrointestinal
Inseticidas/metabolismo
Mariposas/microbiologia
-Filogenia
Bactérias/isolamento & purificação
Bactérias/classificação
Bactérias/genética
Trato Gastrointestinal/microbiologia
Carboxilesterase/genética
Esterases/genética
Índia
Limites: Animais
Masculino
Feminino
Responsável: BR1.1 - BIREME


  2 / 15 LILACS  
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Id: lil-755830
Autor: Alnimr, Amani M.
Título: Dormancy models for Mycobacterium tuberculosis: A minireview
Fonte: Braz. j. microbiol;46(3):641-647, July-Sept. 2015. tab, ilus.
Idioma: en.
Resumo:

Dormancy models for Mycobacterium tuberculosis play important roles in understanding various aspects of tuberculosis pathogenesis and in the testing of novel therapeutic regimens. By simulating the latent tuberculosis infection, in which the bacteria exist in a non-replicative state, the models demonstrate reduced susceptibility to antimycobacterial agents. This minireview outlines the models available for simulating latent tuberculosis both in vitro and in several animal species. Additionally, this minireview discusses the advantages and disadvantages of these models for investigating the bacterial subpopulations and susceptibilities to sterilization by various antituberculosis drugs.

.
Descritores: Antituberculosos/farmacologia
Modelos Animais de Doenças
Tuberculose Latente/patologia
Mycobacterium tuberculosis/crescimento & desenvolvimento
Tuberculose Pulmonar/patologia
-Farmacorresistência Bacteriana
Indicadores e Reagentes/farmacologia
Tuberculose Latente/tratamento farmacológico
Tuberculose Latente/microbiologia
Macaca fascicularis
Mycobacterium tuberculosis/efeitos dos fármacos
Mycobacterium tuberculosis/metabolismo
Oxazinas/farmacologia
Tuberculose Pulmonar/tratamento farmacológico
Tuberculose Pulmonar/microbiologia
Xantenos/farmacologia
Peixe-Zebra
Limites: Animais
Cobaias
Camundongos
Coelhos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


  3 / 15 LILACS  
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Id: lil-744705
Autor: Silva, Camila de Azevedo; Baldim, Letícia Botigeli; Nhoncanse, Geiza César; Estevão, Isabeth da Fonseca; Melo, Débora Gusmão.
Título: Neonatal screening program for hemoglobinopathies in the city of São Carlos, state of São Paulo, Brazil: analysis of a series of cases / Triagem neonatal de hemoglobinopatias no município de São Carlos, São Paulo, Brasil: análise de uma série de casos
Fonte: Rev. paul. pediatr;33(1):19-27, Jan-Mar/2015. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: OBJECTIVE: To analyze the neonatal screening program for hemoglobinopathies in São Carlos, Southeast Brazil, by investigating a series of cases in which the screening test was abnormal. More specifically, it was aimed to know the information regarding the neonatal screening received by mothers at the hospital and at primary health care, in addition to information related to genetic counseling. METHODS: A descriptive study that enrolled 119 mothers, accounting for 73% of all children born between 2010 and 2011 with abnormal results of neonatal screening for hemoglobinopathies. The mothers completed a questionnaire that assessed the information received at hospital and primary health care, and issues related to genetic counseling. Descriptive statistics was performed. RESULTS: Of the 119 participating mothers, 69 (58%) had children with sickle cell trait, 22 (18.5%) with hemoglobin C trait, 18 (15.1%) with alpha thalassemia trait and, in 10 cases (8.4%), the result was inconclusive. At the hospital, 118 mothers (99.2%) received information about where to go to collect the test and 115 (96.6%) were informed about the correct time to collect the test. Only 4 mothers (3.4%) were informed about which diseases are investigated and the risks of not performing the screening. Seventeen mothers (14.3%) recognized the difference between trait and disease, and 42 (35.3%) considered that a positive screening test could have implications for future pregnancies. In 70 cases (58.8%), the child's physician was not informed about the screening test results. CONCLUSIONS: The neonatal screening program needs further improvement. In both scenarios investigated, health professionals demonstrated a lack of training in providing information to mothers and families. .

OBJETIVO: Fazer uma análise do programa de triagem neonatal de hemoglobinopatias no município de São Carlos, São Paulo, Brasil, por meio da investigação de série de casos cujo resultado do teste de rastreio foi alterado. Objetivou-se conhecer as informações a respeito da triagem neonatal recebidas pelas mães na maternidade e na atenção primária à saúde, além das informações relacionadas à orientação genética. MÉTODOS: Estudo descritivo, no qual participaram 119 mães cujos filhos apresentaram teste de triagem de hemoglobinopatia alterado, o que correspondeu a 73% das crianças nascidas entre 2010 e 2011 com resultado de triagem neonatal para hemoglobinopatia anormal. As mães responderam um questionário que avaliou informações recebidas na maternidade e na atenção primária à saúde, além de aspectos relacionados à orientação genética. Foi feita estatística descritiva dos dados. RESULTADOS: Das 119 mães participantes, 69 (58%) tinham filhos com traço falciforme, 22 (18,5%) traço C, 18 (15,1%) traço alfatalassêmico e 10 (8,4%) resultado inconclusivo. Na maternidade, 118 mães (99,2%) receberam informação sobre onde ir e 115 (96,6%) foram orientadas sobre o momento correto para coleta do teste. Somente quatro mães (3,4%) foram informadas sobre quais doenças seriam investigadas e os riscos de não fazer o rastreio. Das 119 mães participantes, 17 (14,3%) reconheceram a diferença entre traço e doença e 42 (35,3%) consideraram que um teste alterado poderia ter implicações para futuras gestações. Em 70 casos (58,8%), o médico da criança não foi informado sobre o resultado da triagem. CONCLUSÕES: O programa de triagem neonatal necessita de aperfeiçoamento. Nos dois cenários investigados, os profissionais de saúde carecem de treinamento para orientar mães e famílias. .
Descritores: Antimaláricos/farmacologia
Oxazinas/farmacologia
Plasmodium falciparum/efeitos dos fármacos
Piridinas/farmacologia
-Antimaláricos/síntese química
Antimaláricos/metabolismo
Relação Dose-Resposta a Droga
Testes de Sensibilidade Microbiana
Estrutura Molecular
Oxazinas/síntese química
Oxazinas/metabolismo
Testes de Sensibilidade Parasitária
Piridinas/síntese química
Piridinas/metabolismo
Relação Estrutura-Atividade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  4 / 15 LILACS  
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Id: lil-684524
Autor: Brazilian Journal of Medical and Biological Research; Sheng, W.J.; Jiang, H.; Wu, D.L.; Zheng, J.H..
Título: Early responses of the STAT3 pathway to platinum drugs are associated with cisplatin resistance in epithelial ovarian cancer
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;46(8):650-658, ago. 2013. graf.
Idioma: en.
Projeto: the National Natural Science Foundation of China.
Resumo: Cisplatin resistance remains one of the major obstacles when treating epithelial ovarian cancer. Because oxaliplatin and nedaplatin are effective against cisplatin-resistant ovarian cancer in clinical trials and signal transducer and activator of transcription 3 (STAT3) is associated with cisplatin resistance, we investigated whether overcoming cisplatin resistance by oxaliplatin and nedaplatin was associated with the STAT3 pathway in ovarian cancer. Alamar blue, clonogenic, and wound healing assays, and Western blot analysis were used to compare the effects of platinum drugs in SKOV-3 cells. At an equitoxic dose, oxaliplatin and nedaplatin exhibited similar inhibitory effects on colony-forming ability and greater inhibition on cell motility than cisplatin in ovarian cancer. Early in the time course of drug administration, cisplatin increased the expression of pSTAT3 (Tyr705), STAT3α, VEGF, survivin, and Bcl-XL, while oxaliplatin and nedaplatin exhibited the opposite effects, and upregulated pSTAT3 (Ser727) and STAT3β. The STAT3 pathway responded early to platinum drugs associated with cisplatin resistance in epithelial ovarian cancer and provided a rationale for new therapeutic strategies to reverse cisplatin resistance.
Descritores: Antineoplásicos/administração & dosagem
Resistencia a Medicamentos Antineoplásicos/fisiologia
Neoplasias Epiteliais e Glandulares/tratamento farmacológico
Neoplasias Ovarianas/tratamento farmacológico
/metabolismo
STATABATTOIRS TRANSCRIPTION FACTOR/metabolismo
Transdução de Sinais/efeitos dos fármacos
-Proteínas Reguladoras de Apoptose/genética
Linhagem Celular Tumoral
Ensaios de Migração Celular/métodos
Proliferação de Células/efeitos dos fármacos
Cisplatino/administração & dosagem
Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos
Expressão Gênica/efeitos dos fármacos
Proteínas Inibidoras de Apoptose/genética
Compostos Organoplatínicos/administração & dosagem
Oxazinas/farmacologia
Fator A de Crescimento do Endotélio Vascular/genética
Xantenos/farmacologia
Proteína bcl-X/genética
Limites: Animais
Humanos
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  5 / 15 LILACS  
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Id: lil-643749
Autor: Escribano, Alexandra Ibáñez; Marcel, Alfredo Meneses; Tugores, Yanetsy Machado; Ruiz, Juan José Nogal; Redó, Vicente J Arán; García-Trevijano, José Antonio Escario; Barrio, Alicia Gómez.
Título: Validation of a modified fluorimetric assay for the screening of trichomonacidal drugs
Fonte: Mem. Inst. Oswaldo Cruz;107(5):637-643, Aug. 2012. ilus, tab.
Idioma: en.
Projeto: AECID; . MICINN; . CEI-Campus Moncloa.
Resumo: A fluorimetric microassay that uses a redox dye to determine the viability of the flagellate Trichomonas vaginalis has been optimised to provide a more sensitive method to evaluate potential trichomonacidal compounds. Resazurin has been used in recent years to test drugs against different parasites, including trichomonadid protozoa; however, the reproducibility of these resazurin-based methods in our laboratory has been limited because the flagellate culture medium spontaneously reduces the resazurin. The objective of this work was to refine the fluorimetric microassay method previously developed by other research groups to reduce the fluorescence background generated by the media and increase the sensitivity of the screening assay. The experimental conditions, time of incubation, resazurin concentration and media used in the microtitre plates were adjusted. Different drug sensitivity studies against T. vaginalis were developed using the 5-nitroimidazole reference drugs, new 5-nitroindazolinones and 5-nitroindazole synthetic derivatives. Haemocytometer count results were compared with the resazurin assay using a 10% solution of 3 mM resazurin dissolved in phosphate buffered saline with glucose (1 mg/mL). The fluorimetric assay and the haemocytometer counts resulted in similar percentages of trichomonacidal activity in all the experiments, demonstrating that the fluorimetric microtitre assay has the necessary accuracy for high-throughput screening of new drugs against T. vaginalis.
Descritores: Antitricômonas/farmacologia
Clotrimazol/farmacologia
Metronidazol/análogos & derivados
Metronidazol/farmacologia
Trichomonas vaginalis/efeitos dos fármacos
-Fluorometria
Ensaios de Triagem em Larga Escala
Oxazinas
Testes de Sensibilidade Parasitária
Sensibilidade e Especificidade
Xantenos
Tipo de Publ: Research Support, Non-U.S. Gov't
Estudo de Validação
Responsável: BR1.1 - BIREME


  6 / 15 LILACS  
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Id: lil-643741
Autor: Coban, Ahmet Yilmaz; Uzun, Meltem; Akgunes, Alper; Durupinar, Belma.
Título: Comparative evaluation of the microplate nitrate reductase assay and the rezasurin microtitre assay for the rapid detection of multidrug resistant Mycobacterium tuberculosis clinical isolates
Fonte: Mem. Inst. Oswaldo Cruz;107(5):578-581, Aug. 2012. ilus, tab.
Idioma: en.
Resumo: The microplate nitrate reductase assay (MNRA) and the rezasurin microtitre assay (REMA) were used for the susceptibility testing of 73 clinical isolates and the results were compared with those that were obtained using the Bactec 460 TB and Bactec MGIT 960 systems. The REMA and the MNRA were performed in 96-well plates. For the REMA, the concentrations of isoniazid (INH) and rifampicin (RIF) ranged from 1.0-0.01 µg/mL and 2.0-0.03 µg/mL, respectively. For the MNRA, the INH concentration was between 1.0-0.03 µg/mL and the RIF concentration was between 2.0-0.06 µg/mL. For the MNRA, the sensitivity, specificity, positive predictive value, negative predictive value and INH/RIF agreement were 100/95.6, 97.6/100, 96.8/100, 100/98 and 98.6/98.6, respectively, and for the REMA, they were 100/91.3, 90.4/100, 88.5/100, 100/96.1 and 94.5/97.2, respectively. Our data suggest that these two rapid, low-cost methods may be inexpensive, alternative assays for the rapid detection of multidrug resistant tuberculosis in low-income countries.
Descritores: Antituberculosos/farmacologia
Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos
Isoniazida/farmacologia
Testes de Sensibilidade Microbiana/métodos
Mycobacterium tuberculosis/efeitos dos fármacos
Rifampina/farmacologia
-Mycobacterium tuberculosis/isolamento & purificação
Nitrato Redutase/metabolismo
Oxazinas/metabolismo
Valor Preditivo dos Testes
Kit de Reagentes para Diagnóstico
Sensibilidade e Especificidade
Tuberculose Resistente a Múltiplos Medicamentos/microbiologia
Xantenos/metabolismo
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


  7 / 15 LILACS  
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Id: lil-641007
Autor: Bardella, V. B; Azeredo-Oliveira, M. T. V; Tartarotti, E.
Título: Cytogenetic analysis in the spermatogenesis of Triatoma melanosoma (Reduviidae; Heteroptera)
Fonte: Genet. mol. res. (Online);7(2):326-335, 2008. ilus.
Idioma: en.
Resumo: Triatomines are of great concern in public health because they are vectors of Chagas' disease. This study presents an analysis of the species Triatoma melanosoma. The cytogenetic characteristics of triatomines include holocentric chromosomes, post-reductional meiosis in the sex chromosomes and nucleolar fragmentation in the meiotic cycle. The methodology utilized consisted of the techniques of lacto-acetic orcein staining and silver ion impregnation. The organs analyzed were adult testicles. The results enabled to classify the chromosomes by number and size, being three large, eight medium and one small heterochromosome. The three largest chromosomes and the heterochromosomes showed heteropyknotic chromatin in meiosis. The heterochromosomes in 8.05% of the cells in metaphase I behaved as pseudobivalents, contrasting with 91.95% of the cells with individualized sex chromosomes, confirming the achiasmatic nature of these chromosomes. However, the pseudobivalents occurred prominently in metaphase II (78.38%), this fact probably is related to the post-reductional nature of the sex chromosomes. The nucleolus in T. melanosoma persisted until the diplotene phase after which it began to fragment. Nucleolar corpuscles were observed in metaphases I and II and during anaphases I and II, these characteristics being related to the phenomenon of nucleolar persistence. In the initial spermatids, peripheral silver ion impregnation occurred, which could be analogous to the pre-nucleolar corpuscles observed after fragmentation. Thus, this study extends our knowledge of the characteristics of triatomines, in particular, heteropyknotic degree, kinetic activity, formation of sex chromosome achiasmatic pseudobivalency, confirmation of the fragmentation phenomenon, and post-meiotic nucleolar reactivation.
Descritores: Análise Citogenética/métodos
Espermatogênese/genética
Triatoma/genética
-Cromossomos/genética
Meiose
Oxazinas/química
Cromossomos Sexuais
Testículo/citologia
Testículo/metabolismo
Limites: Animais
Masculino
Responsável: BR26.1 - Biblioteca Central


  8 / 15 LILACS  
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Id: lil-556530
Autor: Campuzano-Martínez, Aidee; Rodríguez-Maciel, J. Concepcion; Lagunes-Tejeda, Ángel; Llanderal-Cázares, Celina; Terán-Vargas, Antonio P; Vera-Graziano, Jorge; Vaquera-Huerta, Humberto; Silva-Aguayo, Gonzalo.
Título: Aptitud biológica de poblaciones de Bemisia tabaci (Gennadius) Biotipo B (Hemiptera: Aleyrodidae) con diferente susceptibilidad al insecticida thiametoxam / Fitness of Bemisia tabaci (Gennadius) B Biotype (Hemiptera: Aleyrodidae) populations with different levels of susceptibility to the thiametoxam insecticide
Fonte: Neotrop. entomol;39(3):430-435, May-June 2010. tab, ilus.
Idioma: es.
Resumo: In two field-collected populations of de Bemisia tabaci (Gennadius) B biotype previously selected with the neonicotinoid insecticide thiamethoxam (NEO-R, NEO-N), and a population susceptible to insecticides (SUSC), the level of response to the insecticide thiametoxam, as well as the life and fertility tables were determined. At LC95, the NEO-R population showed a resistance ratio (RR95) value of 8.8-fold, an intrinsic rate of increase (r m) of 0.72. The fitness of the NEO-R and NEO-N populations in relation to the SUSC was 1.5 and 2.0, respectively. The RR95 value for NEO-N was 1.9-fold; it's r m value was 0.082, while in the SUSC population was 0.041. The developmental time of egg and adult were longer in NEO-R population, while the nymph and pupa lasted longer in the NEO-N and SUSC populations. The developmental time was different in the three NEO-R, NEO-N, and SUSC populations with 19.7 d (315.84 degree days or dd), 15.7 d (250.4 dd) and 18.5 d (296.6 d), respectively. The populations previously selected with thiamethoxam did not reproduce faster than their susceptible counterpart.
Descritores: Hemípteros/efeitos dos fármacos
Inseticidas/farmacologia
Nitrocompostos/farmacologia
Oxazinas/farmacologia
Tiazóis/farmacologia
-Hemípteros/classificação
Hemípteros/fisiologia
Limites: Animais
Responsável: BR1.1 - BIREME


  9 / 15 LILACS  
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Id: lil-530261
Autor: Borra, Ricardo Carneiro; Lotufo, Mônica Andrade; Gagioti, Sonia Maria; Barros, Fabiana de Mesquita; Andrade, Priscila Maria.
Título: A simple method to measure cell viability in proliferation and cytotoxicity assays
Fonte: Braz. oral res;23(3):255-262, 2009. graf, ilus.
Idioma: en.
Resumo: Resazurin dye has been broadly used as indicator of cell viability in several types of assays for evaluation of the biocompatibility of medical and dental materials. Mitochondrial enzymes, as carriers of diaphorase activities, are probably responsible for the transference of electrons from NADPH + H+ to resazurin, which is reduced to resorufin. The level of reduction can be quantified by spectrophotometers since resazurin exhibits an absorption peak at 600 çm and resorufin at 570 çm wavelengths. However, the requirement of a spectrophotometer and specific filters for the quantification could be a barrier to many laboratories. Digital cameras containing red, green and blue filters, which allow the capture of red (600 to 700 çm) and green (500 to 600 çm) light wavelengths in ranges bordering on resazurin and resorufin absorption bands, could be used as an alternative method for the assessment of resazurin and resorufin concentrations. Thus, our aim was to develop a simple, cheap and precise method based on a digital CCD camera to measure the reduction of resazurin. We compared the capability of the CCD-based method to distinguish different concentrations of L929 and normal Human buccal fibroblast cell lines with that of a conventional microplate reader. The correlation was analyzed through the Pearson coefficient. The results showed a strong association between the measurements of the method developed here and those made with the microplate reader (r² = 0.996; p < 0.01) and with the cellular concentrations (r² = 0.965; p < 0.01). We concluded that the developed Colorimetric Quantification System based on CCD Images allowed rapid assessment of the cultured cell concentrations with simple equipment at a reduced cost.
Descritores: Sobrevivência Celular/efeitos dos fármacos
Fibroblastos/efeitos dos fármacos
Indicadores e Reagentes/toxicidade
Oxazinas/toxicidade
Testes de Toxicidade/métodos
Xantenos/toxicidade
-Calorimetria/métodos
Proliferação de Células/efeitos dos fármacos
Células Cultivadas/efeitos dos fármacos
Fibroblastos/citologia
Indicadores e Reagentes/metabolismo
Mucosa Bucal/citologia
Oxazinas/metabolismo
Fotografação/instrumentação
Fotografação/métodos
Xantenos/metabolismo
Limites: Humanos
Responsável: BR1.1 - BIREME


  10 / 15 LILACS  
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Coelho, P. M. Z
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Id: lil-441262
Autor: Kusel, J. R; Oliveira, F. A; Todd, M; Ronketti, F; Lima, S. F; Mattos, A. C. A; Reis, K. T; Coelho, P. M. Z; Thornhill, J. A; Ribeiro, F.
Título: The effects of drugs, ions, and poly-l-lysine on the excretory system of Schistosoma mansoni
Fonte: Mem. Inst. Oswaldo Cruz;101(supl.1):293-298, Oct. 2006. ilus.
Idioma: en.
Resumo: We have been able to label the excretory system of cercariae and all forms of schistosomula, immature and adult worms with the highly fluorescent dye resorufin. We have shown that the accumulation of the resorufin into the excretory tubules and collecting ducts of the male adult worm depends on the presence of extracellular calcium and phosphate ions. In the adult male worms, praziquantel (PZQ) prevents this accumulation in RPMI medium and disperses resorufin from tubules which have been prelabelled. Female worms and all other developmental stages are much less affected either by the presence of calcium and phosphate ions, or the disruption caused by PZQ. The male can inhibit the excretory system in paired female. Fluorescent PZQ localises in the posterior gut (intestine) region of the male adult worm, but not in the excretory system, except for the anionic carboxy fluorescein derivative of PZQ, which may be excreted by this route. All stages of the parasite can recover from damage by PZQ treatment in vitro. The excretory system is highly sensitive to damage to the surface membrane and may be involved in vesicle movement and damage repair processes. In vivo the adult parasite does not recover from PZQ treatment, but what is inhibiting recovery is unknown, but likely to be related to immune effector molecules.
Descritores: Anti-Helmínticos/farmacologia
Polilisina/farmacologia
Praziquantel/farmacologia
Schistosoma mansoni/efeitos dos fármacos
-Corantes Fluorescentes
Oxazinas
Schistosoma mansoni/fisiologia
Limites: Animais
Feminino
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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