Base de dados : LILACS
Pesquisa : D03.383.663.283.240 [Categoria DeCS]
Referências encontradas : 5 [refinar]
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Texto completo SciELO Brasil
Texto completo
Id: lil-614637
Autor: Makpol, Suzana; Zainuddin, Azalina; Chua, Kien Hui; Yusof, Yasmin Anum Mohd; Ngah, Wan Zurinah Wan.
Título: Gamma-tocotrienol modulation of senescence-associated gene expression prevents cellular aging in human diploid fibroblasts
Fonte: Clinics;67(2):135-143, 2012. ilus, graf, tab.
Idioma: en.
Resumo: OBJECTIVE: Human diploid fibroblasts undergo a limited number of cellular divisions in culture and progressively reach a state of irreversible growth arrest, a process termed cellular aging. The beneficial effects of vitamin E in aging have been established, but studies to determine the mechanisms of these effects are ongoing. This study determined the molecular mechanism of γ-tocotrienol, a vitamin E homolog, in the prevention of cellular aging in human diploid fibroblasts using the expression of senescence-associated genes. METHODS: Primary cultures of young, pre-senescent, and senescent fibroblast cells were incubated with γ-tocotrienol for 24 h. The expression levels of ELN, COL1A1, MMP1, CCND1, RB1, and IL6 genes were determined using the quantitative real-time polymerase chain reaction. Cell cycle profiles were determined using a FACSCalibur Flow Cytometer. RESULTS: The cell cycle was arrested in the G0/G1 phase, and the percentage of cells in S phase decreased with senescence. CCND1, RB1, MMP1, and IL6 were upregulated in senescent fibroblasts. A similar upregulation was not observed in young cells. Incubation with γ-tocotrienol decreased CCND1 and RB1 expression in senescent fibroblasts, decreased cell populations in the G0/G1 phase and increased cell populations in the G2/M phase. γ-Tocotrienol treatment also upregulated ELN and COL1A1 and downregulated MMP1 and IL6 expression in young and senescent fibroblasts. CONCLUSION: γ-Tocotrienol prevented cellular aging in human diploid fibroblasts, which was indicated by the modulation of the cell cycle profile and senescence-associated gene expression.
Descritores: Antioxidantes/farmacologia
Senescência Celular/efeitos dos fármacos
Ciclo Celular/efeitos dos fármacos
Cromanos/farmacologia
Fibroblastos/efeitos dos fármacos
Vitamina E/análogos & derivados
beta-Galactosidase/análise
-Análise de Variância
Biomarcadores/análise
Células Cultivadas
Senescência Celular/genética
Ciclo Celular/genética
Colágeno Tipo I/genética
Colágeno Tipo I/metabolismo
Ciclina D1/genética
Ciclina D1/metabolismo
Diploide
Fibroblastos/citologia
Fibroblastos/metabolismo
/genética
INTERLEUKIN-ABDOMEN, ACUTE/genética
/metabolismo
INTERLEUKIN-ABDOMEN, ACUTE/metabolismo
Metaloproteinase 1 da Matriz/genética
Metaloproteinase 1 da Matriz/metabolismo
RNA Mensageiro/metabolismo
Proteína do Retinoblastoma/genética
Proteína do Retinoblastoma/metabolismo
Regulação para Cima/efeitos dos fármacos
Vitamina E/farmacologia
beta-Galactosidase/metabolismo
Limites: Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: lil-443676
Autor: Perez, D. D; Leighton, F; Aspee, A; Aliaga, C; Lissi, E.
Título: A comparison of methods employed to evaluate antioxidant capabilities
Fonte: Biol. Res;33(2):71-77, 2000. tab.
Idioma: en.
Resumo: Three different methodologies frequently employed to evaluate the indexes that report the antioxidant capabilities of pure compounds and/or complex mixtures of antioxidants are applied to a series of mono- and polyphenols, as well as to two wine (red and white) samples. These methodologies are based on the bleaching of a stable radical, the effect of the additive upon luminol chemiluminescence induced by peroxyl radicals, and the effect of the additive upon the bleaching of the fluorescence from a dye molecule. Widely different responses are obtained from the different methodologies. These differences are interpreted in terms of the different factors (stoichiometric factors and/or reactivities) that determines the indexes evaluated by these different methodologies.
Descritores: Antioxidantes/química
Cromanos/química
Espectrometria de Fluorescência/métodos
Medições Luminescentes
Peróxidos/química
Vinho/análise
-Flavonoides
Luminol
Fenóis
Fatores de Tempo
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
Texto completo
Id: lil-440783
Autor: Salomão, Alberto Bicudo; Aguilar-Nascimento, José Eduardo; Percário, Sandro; Sano, Victor; Marques, Nicole Ribeiro; Dias, Claudia Cristina Gomes de Oliveira.
Título: Intestinal intraluminal injection of glutamine increases trolox total equivalent antioxidant capacity (TEAC) in hepatic ischemia-reperfusion
Fonte: Acta cir. bras;21(supl.4):69-73, 2006. tab, graf.
Idioma: en.
Resumo: PURPOSE: To evaluate the effects of intraluminal injection of glutamine on the serum trolox equivalent antioxidant capacity in an experimental model of ischemia-reperfusion of the liver observing the applicability of modifications on the original assay method. METHODS: Thirty Wistar rats underwent laparotomy to perform a 20 cm blind sac of small bowel and occlusion of the hepatic hilo for 30 minutes and reperfusion for 5 minutes. Into the gut sac it was injected glutamine (glutamine group, n=10) or distilled water (control group, n=10). Ten other animals (sham group) underwent laparotomy without artery occlusion. Blood samples were collected for trolox equivalent antioxidant capacity assays in different temperature conditions, reagent quantities and time for spectrophotometer readings. RESULTS: Total antioxidant capacity was significantly greater in glutamine group than in both control group (1,60[1,55-1,77] vs 1,44[1,27-1,53]) and sham group (1,60[1,55-1,77] vs 1,48[1,45-1,59]). CONCLUSION: Glutamine enhanced serum antioxidant capacity. The assay technique consistently reflected the changes in the antioxidant defenses in this experimental model.

OBJETIVO: Avaliar em um modelo experimental de isquemia-reperfusão hepática os efeitos da injeção intraluminal de glutamina na capacidade anti-oxidante total em equivalência ao trolox (TEAC) do plasma, verificando a aplicabilidade de modificações ao método original de dosagem. MÉTODOS: Trinta ratos Wistar foram submetidos a laparotomia e confecção de uma alça fechada de 20 cm de comprimento envolvendo o intestinal delgado distal seguido do clampeamento do hilo hepático por 30 minutos e reperfusão por 5 minutos. Na alça fechada foi injetada glutamina (grupo glutamina; n=10) ou água destilada (grupo controle; n=10). Em dez animais (grupo sham) não foi realizado clampeamento hilar. Coletou-se sangue para dosagem da capacidade antioxidante total em equivalência ao trolox em condições modificadas de temperatura, proporções relativas dos reagentes e tempo de leitura sob espectrofotometria. RESULTADOS: A capacidade antioxidante total foi significantemente maior (p<0.05) no grupo glutamina que no grupo controle (1,60[1,55-1,77] vs 1,44[1,27-1,53]) e grupo sham (1,60[1,55-1,77] vs 1,48[1,45-1,59]). Não houve diferenças estatísticas entre o grupo controle e o grupo sham. CONCLUSÃO: A glutamina melhorou a capacidade anti-oxidante total plasmática. O método de dosagem refletiu consistentemente alterações na defesa anti-oxidante nesse modelo experimental.
Descritores: Antioxidantes/metabolismo
Cromanos/sangue
Glutamina/farmacologia
Intestino Delgado/efeitos dos fármacos
Fígado/irrigação sanguínea
Traumatismo por Reperfusão/tratamento farmacológico
-Antioxidantes/química
Cromanos/química
Modelos Animais de Doenças
Distribuição Aleatória
Ratos Wistar
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
Criddle, D. N
Texto completo
Id: lil-265854
Autor: Criddle, D. N; Moura, R. S. de.
Título: Vasorelaxant effects of the potassium channel opener SR 47063 on the isolated human saphenous vein and rat aorta
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;33(8):961-6, Aug. 2000. tab, graf.
Idioma: en.
Resumo: The vasorelaxant effects of SR 47063 (4-(2-cyanimino-1,2-dihydropyrid-1-yl)-2,2-dimethyl-6-nitrochromene), a new K+-channel opener structurally related to levcromakalim, were examined in isolated human saphenous vein (HSV) and rat aorta (RA). HSV or RA rings were precontracted with either KCl or noradrenaline and cumulative relaxant concentration-response curves were obtained for SR 47063 (0.1 nM to 1 µM) in the presence or absence of 3 µM glibenclamide. SR 47063 potently relaxed HSV and RA precontracted with 20 mM (but not 60 mM) KCl or 10 µM noradrenaline in a concentration-dependent manner, showing slightly greater activity in the aorta. The potency of the effect of SR 47063 on HSV and RA was 12- and 58-fold greater, respectively, than that reported for the structurally related K+-channel opener levcromakalim. The vasorelaxant action of SR 47063 in both blood vessels was strongly inhibited by 3 µM glibenclamide, consistent with a mechanism of action involving ATP-dependent K+-channels
Descritores: Aorta/efeitos dos fármacos
Cromanos/farmacologia
Veia Safena/efeitos dos fármacos
Vasodilatadores/farmacologia
-Cromanos/antagonistas & inibidores
Glibureto/farmacologia
Norepinefrina
Ratos Wistar
Vasodilatadores/antagonistas & inibidores
Limites: Seres Humanos
Animais
Masculino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-161667
Autor: Romay, C; Pascual, C; Lissi, E. A.
Título: The reaction between ABTS radical cation and antioxidants and its use to evaluate the antioxidant status of serum samples
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;29(2):175-83, Feb. 1996. graf, tab.
Idioma: en.
Resumo: The 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical cation can be generated by incubation of ABTS and 2,2'-azo-bis(2-amidinopropane) at 45 degrees Celsius. The ABTS radical cation is stable for several minutes at room temperature and reacts quantitatively and instantaneously with several antioxidants, such as Trolox, ascorbic acid, uric acid, cysteine, glutathione and bilirubin. In contrast, the ABTS radical cation reacts slowly with albumin. When serum is added to a solution of the ABTS radical cation, the bleaching of the radical follows biphasic kinetics, with a fast decay followed by a slow decay that takes place within several minutes. The fast decay is primarily due to uric acid, while the slow decay is related to the protein content of the sample. We propose that this procedure can provide an independent and simultaneous evaluation of the low molecular weight and protein antioxidants present in biological samples such as serum.
Descritores: Ácidos Sulfônicos/metabolismo
Antioxidantes/farmacologia
Indicadores e Reagentes/metabolismo
-Ácido Ascórbico/sangue
Ácido Úrico/sangue
Bilirrubina/sangue
Cromanos/farmacologia
Cisteína/sangue
Glutationa/sangue
Temperatura Ambiente
Fatores de Tempo
Limites: Seres Humanos
Masculino
Feminino
Responsável: BR1.1 - BIREME



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