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Pesquisa : D03.383.663.283.240.190 [Categoria DeCS]
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Id: biblio-1249171
Autor: Amparo, Tatiane Roquete; Rodrigues, Ivanildes Vasconcelos; Seibert, Janaína Brandão; Almeida, Tamires Cunha; Cabral, Vivette Appolinário Rodrigues; Vieira, Paula Melo de Abreu; Brandão, Geraldo Célio; Oliveira, Mauro Lúcio Gonçalves de; Silva, Glenda Nicioli da; Santos, Orlando David Henrique dos; Vieira Filho, Sidney Augusto; Teixeira, Luiz Fernando Medeiros; Souza, Gustavo Henrique Bianco de.
Título: Antibacterial substances from leaves of Protium spruceanum (Burseraceae): in vitro and in silico evaluation
Fonte: Braz. J. Pharm. Sci. (Online);56:e18474, 2020. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa de Minas Gerais.
Resumo: Due to the increase of bacterial resistance, the search for new antibiotics is necessary and the medicinal plants represent its most important source. The aim of this study was to evaluate the antibacterial property of extract and fractions from Protium spruceanum leaves, against pathogenic bacteria. By means of diffusion and microdilution assays, the crude extract was active against the nine bacteria tested being the hydromethanolic fraction the most active. During phytochemical procedures, procyanidin (1) and catechin (2) were identified as the main antibacterial constituents of this fraction. In silico results obtained using PASSonline tool indicated 1 and 2 as having good potential to interact with different targets of currently used antibiotics. These results no indicated potential to none DNA effect and indicated the cell wall as mainly target. Electrophoresis result supported that had no DNA damage. Cell wall damage was confirmed by propidium iodide test that showed increased membrane permeability and by cell surface deformations observed in scanning electronic microscopy. The in vitro assays together with the in silico prediction results establish the potential of P. spruceanum as source of antibacterial compounds that acts on important bacterial targets. These results contribute to the development of natural substances against pathogenic bacteria and to discovery of new antibiotics.
Descritores: Plantas Medicinais/efeitos adversos
Técnicas In Vitro/métodos
Extratos Vegetais/análise
Catequina
Antibacterianos/análise
-Simulação por Computador
Microscopia Eletrônica de Varredura/métodos
Folhas de Planta/classificação
Burseraceae/classificação
Compostos Fitoquímicos
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


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Id: biblio-950715
Autor: Sytar, Oksana; Borankulova, Asel; Hemmerich, Irene; Rauh, Cornelia; Smetanska, Iryna.
Título: Effect of chlorocholine chlorid on phenolic acids accumulation and polyphenols formation of buckwheat plants
Fonte: Biol. Res;47:1-7, 2014. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Effect of chlorocholine chloride (CCC) on phenolic acids composition and polyphenols accumulation in various anatomical parts (stems, leaves and inflorescences) of common buckwheat (Fagopyrum esculentum Moench) in the early stages of vegetation period were surveyed. RESULTS: Treatment of buckwheat seeds with 2% of CCC has been increased content of total phenolics in the stems, leaves and inflorescences. On analyzing the different parts of buckwheat plants, 9 different phenolic acids - vanilic acid, ferulic acid, trans-ferulic acid, chlorogenic acid, salycilic acid, cinamic acid, p-coumaric acid, p-anisic acid, methoxycinamic acid and catechins were identified. The levels of identified phenolic acids varied not only significantly among the plant organs but also between early stages of vegetation period. Same changes as in contents of chlorogenic acid, ferulic acid, trans-ferulic acid were found for content of salycilic acid. The content of these phenolic acids has been significant increased under effect of 2% CCC treatment at the phase I (formation of buds) in the stems and at the phase II (beginning of flowering) in the leaves and then inflorescences respectively. The content of catechins as potential buckwheat antioxidants has been increased at the early stages of vegetation period after treatment with 2% CCC. CONCLUSIONS: The obtained results suggest that influence of CCC on the phenolics composition can be a result of various mechanisms of CCC uptake, transforming and/or its translocation in the buckwheat seedlings.
Descritores: Clormequat/farmacologia
Fagopyrum/efeitos dos fármacos
Polifenóis/biossíntese
Hidroxibenzoatos/metabolismo
-Propionatos
Sementes/efeitos dos fármacos
Sementes/metabolismo
Catequina/análise
Ácido Clorogênico/análise
Cromatografia Líquida de Alta Pressão
Compostos de Tungstênio
Caules de Planta/efeitos dos fármacos
Caules de Planta/metabolismo
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/metabolismo
Fagopyrum/crescimento & desenvolvimento
Fagopyrum/metabolismo
Ácidos Cumáricos/análise
Inflorescência/efeitos dos fármacos
Inflorescência/metabolismo
Éteres de Hidroxibenzoatos/análise
Hidroxibenzoatos/química
Molibdênio
Antioxidantes/análise
Antioxidantes/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950733
Autor: Cetin, Emine Sema.
Título: Induction of secondary metabolite production by UV-C radiation in Vitis vinifera L. Öküzgözü callus cultures
Fonte: Biol. Res;47:1-7, 2014. tab.
Idioma: en.
Resumo: BACKGROUND: The aim of the present work was to examine the role of UV-C irradiation on the production of secondary metabolites (total phenolic, total flavanols, total flavonols, catechin, ferulic acid and trans-resveratrol in phenolic compounds and α-, ß-, γ- δ-tocopherols) in callus cultures. Studies on the effects of UV-C treatment on callus culture are seldom and generally focused on UV-B. However UV-C radiation play an important role in accumule secondary metabolites. RESULTS: In this study, callus cultures from Öküzgözü grape cultivar were initiated from leaf petiole explants. Calli formed after 6 weeks on the medium supplemented with 0.5 mg L-1 benzylaminopurine (BA), 0.5 mg L-1 indole acetic acid (IAA) on B5 media. Callus tissues were exposed to UV-C irradiation at 10, 20 and 30 cm distances from the UV source for 5 and 10 minutes and samples were collected at hours 0, 24 and 48. CONCLUSIONS: The greatest total phenolic content (155.14 mg 100 g-1) was detected in calli exposed to UV-C for 5 min from 30 cm distance and sampled after 24 h. 24 h and 48 h incubation times, 30 cm and 5 min were the most appropriate combination of UV-C application in total flavanol content. Maximum total flavonol content (7.12 mg 100 g-1) was obtained on 0 h, 5 min and 20 cm combination. The highest (+)- catechin accumulation (8.89 mg g-1) was found in calli with 10 min UV-C application from 30 cm distance and sampled after 48 h. Ferulic acid content increased 6 fold in Öküzgözü callus cultures (31.37 µg g-1) compared to the control group. The greatest trans-resveratrol content (8.43 µg g-1) was detected in calli exposed to UV-C for 5 min from 30 cm distance and sampled after 24 h. The highest α-tocopherol concentration was found in calli exposed to UV-C for 10 min from 30 cm distance and sampled after 24 h. As a conclusion, it was showed that UV-C radiation had remarkable promoting effects on the accumulation of secondary metabolites in the calli of Öküzgözü grape cultivar.
Descritores: Raios Ultravioleta
Folhas de Planta/efeitos da radiação
Produtos Agrícolas/efeitos da radiação
Vitis/efeitos da radiação
Técnicas de Embriogênese Somática de Plantas/métodos
Metabolismo Secundário/efeitos da radiação
-Fenóis/análise
Estilbenos/análise
Flavonoides/análise
Catequina/análise
Cromatografia
Folhas de Planta/metabolismo
Folhas de Planta/química
Produtos Agrícolas/fisiologia
Ácidos Cumáricos/análise
Vitis/metabolismo
Vitis/química
Tocoferóis/análise
Flavonóis/análise
Metabolismo Secundário/fisiologia
Resveratrol
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1153296
Autor: Yathzamiry, Valdez-Guerrero Daisy; Cecilia, Esparza-González Sandra; Antonio, Morlett-Chávez Jesús; Daniel, Nery-Flores Sendar; Carolina, Flores-Gallegos Adriana; Alberto, Ascacio-Valdés Juan; Raúl, Rodríguez-Herrera.
Título: Isolation of Polyphenols from Soursop Annona muricata L Leaves Using Green Chemistry Techniques and their Anticancer Effect
Fonte: Braz. arch. biol. technol;64:e21200163, 2021. tab, graf.
Idioma: en.
Projeto: The Secretary of Agriculture, Fishing and Livestock-Mexico.
Resumo: HIGHLIGHTS Isolate, fractionate and characterize extracts obtained from soursop leaves. Use of emerging green technologies such as microwave-ultrasound hybridization. The extracts contain kaempferol, procyanidins, catechin, and quercetin. The total ethanolic extract demonstrates cytotoxic effect on HeLa cells.

Abstract Cervical cancer is classified as the fourth most common malignancy in women. Natural compounds are a therapeutic alternative in cancer therapy. The aim of the study is to isolate, fractionate, and characterize extracts obtained from soursop leaves (Annona muricata L.) and determine their cytotoxic effect against HeLa cervical cancer cells and non-carcinogenic fibroblast 3T3 cells. The phytochemicals of soursop leaves were extracted through emerging green technologies such as the novel use of microwave-ultrasound hybridization and the use of environmentally friendly solvents (water and ethanol), in addition to the purification of extracts enriched in polyphenols by liquid chromatography with Amberlite XAD-16. Total aqueous and ethanolic extract were purified, as well as the fraction one of each extract. The extracts recovered from soursop leaves contained kaempferol and its isomers, procyanidins, catechin, and quercetin. The viability of the cells was determined with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. HeLa and 3T3 cells were exposed to concentrations of 25, 50, 75, 100, 150, 200, and 250 ppm of a solution of soursop leaf extract powder. The MTT assay showed that soursop leaf extracts were toxic to both cell lines in general, however, the ethanolic extract at 25 and 50 ppm demonstrated inhibition in cell viability against the HeLa cancer line and low cytotoxicity for 3T3 fibroblast cells. In conclusion, the novel microwave-ultrasound hybridization technology allows the extraction of polyphenols that may have a potential cytotoxic effect on cancer cells.
Descritores: Células HeLa
Annona/química
Polifenóis/isolamento & purificação
Compostos Fitoquímicos/farmacologia
Antineoplásicos Fitogênicos/isolamento & purificação
-Extratos Vegetais/farmacologia
Catequina/química
Cromatografia Líquida/métodos
Etanol
Antineoplásicos Fitogênicos/farmacologia
Limites: Humanos
Feminino
Responsável: BR1.1 - BIREME


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Id: lil-743839
Autor: González Martínez, Gerardo; Núñez Troconis, José.
Título: Tratamiento de las verrugas genitales: una actualización / Genital wart treatment: an update
Fonte: Rev. chil. obstet. ginecol;80(1):76-83, 2015. ilus.
Idioma: es.
Resumo: La infección por el Virus del Papiloma Humano es una afección altamente prevalente a nivel mundial y una de sus consecuencias es la verruga genital externa o condiloma genital. El tratamiento de estas lesiones ha sido tradicionalmente difícil, debido a la alta recurrencia de las lesiones. Se revisan los aspectos más novedosos del tratamiento de las verrugas genitales. Las sinecatequinas y una nueva formulación de Imiquimod al 3,75% resaltan como las terapéuticas más novedosas, mientras que la terapia fotodinámica carece de evidencia científica adecuada como para recomendar su uso rutinario. Las vacunas profilácticas y terapéuticas parecen poseer un gran potencial pero aún se encuentran en fases iniciales de investigación. Es deseable estudios con un mayor número de pacientes y un seguimiento más prolongado, que permita una comparación directa de la efectividad entre las diferentes técnicas terapéuticas a corto y largo plazo.

Human Papillomavirus infection is a highly prevalent condition worldwide whose frequent consequence is the external genital wart or genital condyloma. The treatment for this condition has been traditionally difficult due to the recurrence of the lesions. The most innovative aspects of the treatment of genital wart, are reviewed. The sinecatechins and a new formulation of Imiquimod 3.75% stand out as the most innovative therapeutic, while photodynamic therapy lacks adequate scientific evidence to recommend its routine use. The prophylactic and therapeutic vaccines seem to have great potential but are still in early stages of research. Studies should be conducted with larger patient samples and longer follow-ups to allow comparison of the effectiveness among the different therapeutic techniques in the short and long term.
Descritores: Condiloma Acuminado/terapia
Alphapapillomavirus
Doenças dos Genitais Femininos/terapia
-Fotoquimioterapia
Podofilina
Ácido Tricloroacético
Condiloma Acuminado/diagnóstico
Catequina/uso terapêutico
Vacinação
Crioterapia
Infecções por Papillomavirus/terapia
Eletrocoagulação
Imiquimode/uso terapêutico
Doenças dos Genitais Masculinos/terapia
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


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Id: lil-646617
Autor: Valverde García, Paolo.
Título: La epicatequina: un flavonoide para recordar / The epicatechin: a flavonoid to remember
Fonte: ReNut;1(1):7-10, 2007. tab.
Idioma: es.
Descritores: Antioxidantes
Catequina
Flavonoides
Limites: Humanos
Tipo de Publ: Revisão
Responsável: PE1.1 - Oficina Universitária de Biblioteca


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Id: lil-758732
Autor: Guija-Poma, Emilio; Inocente-Camones, Miguel Ángel; Ponce-Pardo, John; Zarzosa-Norabuena, Edwin.
Título: Evaluación de la técnica 2, 2-Difenil-i-Picrilhidrazilo (DPPH) para determinar capacidad antioxidante / Evaluation of Technical 2, 2 - diphenyl -i - Picrilhidrazilo (DPPH ) to determine antioxidant capacity
Fonte: Horiz. méd. (Impresa);15(1):57-60, Ene.-Mar.2015. tab.
Idioma: es.
Resumo: Este estudio evalúa la actividad antioxidante usando el método del radical libre 2,2-difenil-1-picrilhidracilo (DPPH). Este método se utiliza para determinar la capacidad antioxidante de alimentos y compuestos sintéticos; para este fin se ha hecho uso de este radical libre en concentraciones comprendidas entre 0,037 y 0,200 mM, así mismo, para llevar a cabo esta evaluación se utilizó los estándares de catequina y epicatequina en un rango de concentración que estuvo entre 6,67 x 10-3 y 2,2 x 10-2 mM. Los valores de IC50 para ambos estándares fueron dependientes de la concentración de DPPH, habiéndose observado que el valor anterior aumenta al incrementar la concentración de DPPH. El resultado obtenido en este estudio indica la importancia de la concentración del DPPH para evaluar la capacidad antioxidante...

We assessed the technique using free radical 2,2-diphenyl-1-picrilhidrazil (DPPH) used to determine the antioxidant capacity of foods and synthetic compounds; for that purpose this free radical has been used in concentrations between 0.037 and 0.2 mM, likewise, to perform this evaluation the catechin and epicatechin standards have been used in a concentration range of 6.67 x 10(-3) and 2.2 x 10(-2) mM. C1(50) values for both standards were dependent on the concentration of DPPH, having observed that the aboye value increases with increasing concentration of DPPH, for which reason it is suggested that all research use a single concentration of DPPH in the reaction medium...
Descritores: Antioxidantes
Catequina
Limites: Humanos
Responsável: PE264.1 - Biblioteca


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Id: biblio-1040132
Autor: Freitas, Luana Cristina de; Sousa, Luiz Gustavo de; Leite, Guilherme Luciano; Scalize, Priscilla Hakime; Pitol, Dimitrius Leonardo; Righetti, Mariah Acioli; Ricardo, Vitória; Mello, Edneia Corrêa de; Bombonato-Prado, Karina Fittipaldi; Regalo, Simone Cecilio Hallak; Siessere, Selma.
Título: Influence of green tea extract with different concentrations of epigallocatechin gallate on calvaria bone repair of ovariectomized rats / Influencia del extracto de té verde con diferentes concentraciones de galato de epigalocatequina sobre la reparación ósea de calvaria de ratas ovariectomizadas
Fonte: Int. j. morphol;37(4):1325-1330, Dec. 2019. graf.
Idioma: en.
Projeto: (São Paulo Research Foundation.
Resumo: Impairing osteoporosis progression is a challenge, and recently the role of antioxidants has been associated to bone metabolism. Green tea extract is rich in catechins, especially epigallocatechin gallate (EGCG), which may help control osteoporosis damage in bone tissue. This investigation evaluated the efficacy of green tea ingestion containing different concentrations of EGCG in calvaria bone repair of ovariectomized rats. Wistar rats (n=15) were ovariectomized and divided into 3 groups: ovariectomized (OVX), ovariectomized + GTE 15 % EGCG (OVX/GTE15), and ovariectomized + GTE 94 % EGCG (OVX/GTE94). Green tea extract was administered by gavage in the concentration of 50 mg/kg and sham group (n=5) received water. Bone defects were performed in the calvaria 60 days after ovariectomy followed by 4 weeks until euthanasia. Bone samples were collected to perform qualitative and quantitative histological analysis of bone formation. Data obtained were submitted to normality and ANOVA statistical test for p<0.05. The mean values of neoformed bone for Sham, OVX, OVX/GTE15 and OVX/GTE94 were respectively: 21.11 ± 3.91; 19.92 ± 2.20; 33.05 ± 1.26 e 34.75 ± 0.54 (p<0.05). Results show that continuous ingestion of green tea extract immediately after ovariectomy shows positive effects in the prevention of bone loss in osteoporosis, even with low concentrations of EGCG.

La disminución en la progresión de la osteoporosis es un desafío, y recientemente el papel de los antioxidantes se ha asociado al metabolismo óseo. El extracto de té verde es rico en catequinas, especialmente el galato de epigalocatequina (EGCG), lo que puede ayudar a controlar el daño de la osteoporosis en el tejido óseo. Esta investigación evaluó la eficacia de la ingesta de té verde con diferentes concentraciones de EGCG en la reparación ósea de calvaria de ratas ovariectomizadas. Las ratas Wistar (n = 15) fueron ovariectomizadas y divididas en 3 grupos: ovariectomizadas (OVX), ovariectomizadas + GTE 15 % EGCG (OVX / GTE15), y ovariectomizadas + GTE 94 % EGCG (OVX / GTE94). El extracto de té verde se administró por sonda en una concentración de 50 mg/kg y el grupo simulado (n = 5) recibió agua. Los defectos óseos se realizaron en la calvaria 60 días después de la ovariectomía, seguido de 4 semanas hasta la eutanasia. Se obtuvieron muestras de hueso para realizar un análisis histológico cualitativo y cuantitativo de la formación ósea. Los datos obtenidos se sometieron a normalidad y prueba estadística ANOVA (p<0,05). Los valores medios de hueso neoformado para Sham, OVX, OVX / GTE15 y OVX / GTE94 fueron: 21,11 ± 3,91; 19,92 ± 2,20; 33,05 ± 1,26 y 34,75 ± 0,54 (p <0,05), respectivamente. Los resultados muestran que la ingesta continua de extracto de té verde, inmediatamente después de la ovariectomía, muestra efectos positivos en la prevención de la pérdida ósea ocurrida en la osteoporosis, incluso con concentraciones bajas de EGCG.
Descritores: Chá/química
Regeneração Óssea
Extratos Vegetais/farmacologia
Catequina/análogos & derivados
Catequina/metabolismo
-Osteoporose/patologia
Osteoporose/tratamento farmacológico
Extratos Vegetais/uso terapêutico
Ovariectomia
Ratos Wistar
Limites: Animais
Feminino
Ratos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1015957
Autor: Li, Jiajia; Xiao, Qiong; Huang, Yufeng; Ni, Hui; Wu, Changzheng; Xiao, Anfeng.
Título: Tannase application in secondary enzymatic processing of inferior Tieguanyin oolong tea
Fonte: Electron. j. biotechnol;28:87-94, July. 2017. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Science and Technology Planning Project of Fujian Province.
Resumo: Background: Inferior Tieguanyin oolong tea leaves were treated with tannase. The content and bioactivity of catechins in extracts from the treated tea leaves were investigated to assess the improvement in the quality of inferior Tieguanyin oolong tea. Results: Analysis showed that after treatment, the esterified catechin content decreased by 23.5%, whereas non-galloylated catechin and gallic acid contents increased by 15.3% and 182%, respectively. The extracts from tannase-treated tea leaves showed reduced ability to bind to BSA and decreased tea cream levels. The extracts also exhibited increased antioxidant ability to scavenge OH and DPPH radicals, increased ferric reducing power, and decreased inhibitory effects on pancreatic α-amylase and lipase activities. Conclusions: These results suggested that tannase treatment could improve the quality of inferior Tieguanyin oolong tea leaves.
Descritores: Chá/enzimologia
Hidrolases de Éster Carboxílico/metabolismo
-Chá/metabolismo
Chá/química
Temperatura
Catálise
Catequina/análise
Folhas de Planta/enzimologia
Fermentação
Hidrólise
Lipase/antagonistas & inibidores
Lipase/metabolismo
Antioxidantes
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1011267
Autor: Silva, E. C. B; Arruda, L. C. P; Vieira, J. I. T; Soares, P. C; Guerra, M. M. P.
Título: (+)-Catechin and (-)-epigallocatechin gallate: are these promising antioxidant therapies for frozen goat semen? / (+)-Catequina e (-)-epigalocatequina galato: essas são promissoras terapias antioxidantes para a congelação de sêmen caprino?
Fonte: Arq. bras. med. vet. zootec. (Online);71(2):521-528, mar.-abr. 2019. tab.
Idioma: en.
Resumo: The aim of this study was to evaluate the effects of different concentrations of (+)-catechin or (-)-epigallocatechin gallate (EGCG) on goat semen freezability. Poolsof semen were processed (Experiment 1: 0, 15, 25, 50, 75, or 100µM (+)-catechin; Experiment 2: 0, 15, 25, 50, 75, or 100µM EGCG) and frozen. After thawing, the samples were evaluated for kinematics, plasma membrane (PMi) and acrosome integrity, morphology, and oxidative stress, at 0 and 1h. In Experiment 1, at 0h, VSL and VAP were greater (P<0.05) with 15µM than with 50 and 100; WOB was lower (P<0.05) with 100µM than with 0, 15, and 25; and BCF was higher (P<0.05) with 75 and 100µM than with 0. In turn, in Experiment 2, progressive motility was higher (P<0.05) with0 and 15µM than with50 and 75; LIN was lower (P<0.05) with75 and100µM than with0 and 15; WOB was higher (P<0.05) with0 and 15µM; and PMi was greater (P<0.05) with100µM than 0. Thus, (+)-catechin or EGCG at higher concentrations inhibits the kinematics of frozen goat sperm, in a transitory way, and 100µM of EGCG preserves the PMi.(AU)

Objetivou-se avaliar o efeito de diferentes concentrações de (+)-catequina ou (-)-epigalocatequina galato (EGCG) sobre a congelabilidade do sêmen caprino. Poolsseminais foram processados (experimento 1: 0, 15, 25, 50, 75 ou 100µM de (+)-catequina; experimento 2: 0, 15, 25, 50, 75 ou 100µM de EGCG) e congelados. Após a descongelação, foram avaliadas a cinética, a integridade de membrana plasmática (iMP) e acrossomal, a morfologia e o estresse oxidativo, a zero e a uma hora. No experimento 1, a zero hora, VSL e VAP foram maiores (P<0,05) com 15µM do que com 50 e100; WOB foi menor (P<0,05) com 100µM do que com 0, 15 e 25; e BCF foi maior (P<0,05) com 75 e 100µM do que com 0. No experimento 2, a motilidade progressiva foi maior (P<0,05) com 0 e 15µM do que com 50 e 75; LIN foi menor (P<0,05) com 75 e 100µM do que com 0 e 15; WOB foi maior (P<0,05) com 0 e 15µM; e iMP foi maior (P<0,05) com 100µM do que com 0. Assim, (+)-catequina ou EGCG em altas concentrações inibem, transitoriamente, a cinética de espermatozoides congelados caprinos, e 100µM de EGCG preserva a iMP.(AU)
Descritores: Preservação do Sêmen/métodos
Preservação do Sêmen/veterinária
Flavonoides/farmacologia
Cabras
Catequina/farmacologia
Criopreservação/veterinária
-Estresse Oxidativo
Limites: Animais
Masculino
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde