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Id: biblio-1284586
Autor: Dranguet Aguilar, Duvia; Rodríguez Martínez, Madelín; García Rodríguez, Yuramys Irma.
Título: Plantas medicinales con propiedades antidiabéticas / Medicinal plants with antidiabetic properties.
Fonte: Artemisa; I Jornada Científica de Farmacología y Salud. Fármaco Salud Artemisa 2021; 2021. [1-16] p. tab.
Idioma: es.
Resumo: En este artículo se expone una revisión bibliográfica realizada con el objetivo de actualizar los conocimientos sobre la actividad antidiabética de las plantas medicinales y los productos naturales obtenidos a partir de ellas. Existe gran variedad de plantas con acciones hipoglicemiante y antidiabética relacionadas con la presencia de determinados metabolitos secundarios, entre los que se encuentran en primer lugar los flavonoides, seguidos de alcaloides, taninos y saponinas. Los resultados obtenidos en esta revisión se fundamentan en estudios in vitro e in vivo que probaron el potencial farmacológico de un elevado número de plantas estudiadas.
Descritores: Plantas Medicinais
Hipoglicemiantes
-Saponinas
Flavonoides
Bases de Dados Bibliográficas
Alcaloides
Medicina Tradicional
Responsável: BR1.1 - BIREME


  2 / 359 LILACS  
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Id: biblio-1047978
Autor: Li, Jun; Zhao, Aichun; Yu, Maode; Li, Yaofeng; Liu, Xiaoqing; Chen, Xiangyun.
Título: Function analysis of anthocyanidin synthase from Morus alba L. by expression in bacteria and tobacco
Fonte: Electron. j. biotechnol;36:9-14, nov. 2018. tab, ilus, graf.
Idioma: en.
Projeto: Science and Technology Department of Guizhou Province; . Fund of Guizhou for Construction of First-class Discipline in China; . Specialized Fund for the Top Talent of Science and Technology in colleges and universities of Guizhou; . Scientific and Technological Projects of Guizhou Province.
Resumo: Background: Flavonoids are a kind of important secondary metabolite and are commonly considered to provide protection to plants against stress and UV-B for a long time. Anthocyanidin synthase (ANS), which encodes a dioxygenase in the flavonoid pathway, catalyzes the conversion of leucoanthocyanidins to anthocyanidins, but there is no direct evidence indicating that it provides tolerance to stress in plants. Results: To investigate whether ANS can increase tolerance to abiotic stress, MaANS was isolated from mulberry fruits and transformed into tobacco. Our results suggested that the bacterially expressed MaANS protein can convert dihydroquercetin to quercetin. Overexpression of MaANS remarkably increased the accumulation of total flavonoids in transgenic lines and anthocyanins in corollas of flowers. Transgenic lines showed higher tolerance to NaCl and mannitol stress. Conclusions: These results indicated that MaANS participates in various dioxygenase activities, and it can protect plants against abiotic stress by improving the ROS-scavenging ability. Thus, this alternative approach in crop breeding can be considered in the improvement of stress tolerance by enriching flavonoid production in plants
Descritores: Oxigenases/metabolismo
Tabaco
Morus/enzimologia
-Oxigenases/genética
Quercetina
Estresse Fisiológico
Bactérias
Flavonoides/metabolismo
Plantas Geneticamente Modificadas
Dioxigenases/metabolismo
Expressão Ectópica do Gene
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1253101
Autor: Tsai, Wen-Che; Chang, Hung-Chi; Yin, Hsin-Yi; Huang, Meng-Chieh; Chandra Agrawal, Dinesh; Wen, Hsiao-Wei.
Título: The protective ability and cellular mechanism of Koelreuteria henryi Dummer flower extract against hydrogen peroxide-induced cellular oxidative damage
Fonte: Electron. j. biotechnol;47:89-99, sept. 2020. ilus, tab, graf.
Idioma: en.
Resumo: BACKGROUND: Koelreuteria henryi Dummer is an indigenous plant in Taiwan. The species has been used in traditional folk medicine for the promotion of liver functions and for treating malaria and urethritis. The present study investigated the antioxidant activity of the flower extract of Koelreuteria henryi Dummer. The extraction conditions were optimized by the contents of total phenolic acids and total flavonoids, and antioxidant activity assays. Moreover, an in vitro study for investigating antioxidant activity of K. henryi flower extract was demonstrated by hydrogen peroxide (H2O2)-induced apoptosis. RESULTS: K. henryi flower extracted for 150 min showed high contents of total phenolic acids and total flavonoids. In an in vitro model, L929 cells were pretreated with K. henryi flower extract, and then treated with H2O2 to induce oxidative damage. Results demonstrated that H2O2-induced apoptosis was inhibited by the treatment of 200 µg/ml K. henryi flower extract through the mitochondria-mediated pathway and mitogen-activated protein kinase (MAPK) pathway. The caspase 8/9 activity and expression of p-p38 and pERK were repressed by K. henryi flower extract. In addition, the prevention of H2O2-induced apoptosis by K. henryi flower extract activated the nuclear factor-erythroid 2-related factor (Nrf2) stress response pathway to transcript heme oxygenase 1 (HO-1). Also, K. henryi flower extract prevented H2O2-induced apoptosis through HO-1 production, as evident by the use of HO-1 inhibitor. CONCLUSIONS: The present study demonstrated that K. henryi flower extract could inhibit the H2O2-induced apoptosis in L929 cells through the activation of the Nrf2/HO-1 pathway.
Descritores: Extratos Vegetais/farmacologia
Estresse Oxidativo/efeitos dos fármacos
Sapindaceae/química
Antioxidantes/farmacologia
-Flavonoides/análise
Western Blotting
Apoptose
Flores/química
Heme Oxigenase-1
Fator 2 Relacionado a NF-E2
Caspase 8
Peróxido de Hidrogênio
Responsável: CL1.1 - Biblioteca Central


  4 / 359 LILACS  
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Laurindo, Francisco Rafael Martins
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Id: biblio-906720
Autor: Fernandes, Carolina Gonçalves; Laurindo, Francisco Rafael Martins.
Título: Estresse oxidativo como mecanismo comum a várias doenças vasculares: uma análise crítica / Oxidative stress as a mechanism common to several cardiovascular diseases: a critical analysis
Fonte: Rev. Soc. Cardiol. Estado de Säo Paulo;28(1), jan.-mar. 2018. ilus.
Idioma: pt.
Resumo: Estresse oxidativo é um conceito em evolução. Muito mais do que um simples desbalanço entre oxidantes e antioxidantes, representa um desequilíbrio de vias de sinalização celular redox. Sinalização redox envolve a produção de espécies reativas de oxigênio, radicalares e não radicalares, assim como vias não radicalares de oxidação por dois elétrons. Está claro que essas espécies podem ser produzidas por mecanismos enzimáticos ancestrais e ubíquos e, portanto, não representam acidentes, tendo vários efeitos fisiológicos benéficos. Novas vias de sinalização redox têm sido descritas, com ênfase no papel de peroxiredoxinas, outras tiolproteínas e estados intermediários de oxidação de tióis. Estes fatos levaram à necessidade de reavaliar a definição e a abrangência do conceito de antioxidantes. Apesar da ineficácia dos estudos clínicos com antioxidantes clássicos, há novas perspectivas, que incluem inibidores de enzimas, geradores de geram oxidantes, como NADPH oxidases e vias mitocondriais. Além disso, o uso de flavonóides e compostos relacionados, capazes de ativar vias horméticas de proteção antioxidante, é um caminho importante. Esses fatos indicam que a ciência da área deve entrar em um novo ciclo de estudos para testar novas intervenções clínicas resultantes desses avanços

Oxidative stress is an evolving concept. Much more than a simple imbalance between oxidants and antioxidants, it represents an imbalance of the cellular redox signaling pathways. Redox signaling involves the production of reactive oxygen species, both radicular and non-radicular, as well as non-radicular two-electron oxidation pathways. It is clear that these species can be produced by ancestral and ubiquitous enzymatic mechanisms, therefore they do not represent accidents, and have various beneficial physiological effects. New redox signaling pathways have been described, with emphasis on the role of peroxiredoxins, other protein thiols, and intermediary states of thiol oxidation. These facts have created a need to reassess the definition and scope of the concept of antioxidants. Despite the inefficiency of clinical studies with classic antioxidants, there are new perspectives, which include oxidant-producing enzyme inhibitors, such as NADPH oxidases and mitochondrial pathways. Furthermore, the use of flavonoids and related compounds, capable of activating hormetic pathways of antioxidant protection, is an important route. These facts indicate that the science in the area should enter a new cycle of studies, testing new clinical interventions resulting from these advances
Descritores: Endotélio Vascular
Doenças Cardiovasculares/fisiopatologia
Estresse Oxidativo/fisiologia
-Oxirredução
Flavonoides
Enzimas
Aterosclerose
Inflamação
Antioxidantes
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Revisão
Responsável: BR44.1 - Serviço de Biblioteca, Documentação Científica e Didática Prof. Dr. Luiz Venere Décourt


  5 / 359 LILACS  
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Svidzinski, Terezinha Inez Estivalet
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Id: biblio-1249172
Autor: Ferreira, Magda Rhayanny Assunção; Santiago, Rosilene Rodrigues; Silva-Rocha, Walicyranison Plínio; Souza, Luanda Barbara Ferreira Canário de; Faria, Maria Graciela Icher; Mello, João Carlos Palazzo de; Langassner, Silvana Maria Zucolotto; Chaves, Guilherme Maranhão; Milan, Eveline Pipolo; Svidzinski, Terezinha Inez Estivalet; Soares, Luiz Alberto Lira.
Título: In vitro antifungal activity and phytochemical characterization of Eugenia uniflora, Libidibia ferrea and Psidium guajava
Fonte: Braz. J. Pharm. Sci. (Online);56:e18456, 2020. tab, graf.
Idioma: en.
Projeto: FACEPE; . CAPES-PROCAD/NF; . CNPq.
Resumo: The aim of this study was evaluate the in vitro antifungal activity of crude extracts from Eugenia uniflora, Libidibia ferrea and Psidium guajava. The extracts were obtained by turbo-extraction using water (AQ) or acetone-water (AC-W) (7:3, v/v) as solvents and lyophilized to obtain the crude extracts (CE). The CE were characterized by UV-Vis, TLC and HPLC. The activity of CEs was investigated against clinical isolates of Candida spp. and the Minimum Inhibitory Concentration (MIC), MIC50 and MIC90 were determinated. The analysis by TLC showed that all CEs presented polyphenols (flavonoids and tannins). The CEs from E. uniflora showed higher amount of polyphenols (30.35 ± 2.15%, AC-W) and the HPLC analysis revealed the tannins in all extracts. The CEs of E. uniflora showed MIC range from 1.9 to 500.0 µg/mL, and lower values of MIC50 and MIC90 against non-albicans Candida isolates. Regarding L. ferrea and P. guajava, the results showing MIC from 3.9 to 1000.0 µg/mL (CE-AQ) against C. albicans. The results demonstrate antifungal performance from CE against various species of Candida spp., suggesting that the herbal species may be use as new potential antifungal agents. Additionally, the polyphenol content can play a pivotal role in the antifungal properties of CE.
Descritores: Técnicas In Vitro/métodos
Extratos Vegetais/efeitos adversos
Polifenóis/análise
Compostos Fitoquímicos
Antifúngicos/administração & dosagem
-Flavonoides/farmacocinética
Testes de Sensibilidade Microbiana/métodos
Cromatografia Líquida de Alta Pressão/métodos
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


  6 / 359 LILACS  
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Id: biblio-1249214
Autor: Verma, Kanika; Mahalapbutr, Panupong; Suriya, Utid; Somboon, Tuanjai; Aiebchun, Thitinan; Shi, Liyi; Maitarad, Phornphimon; Rungrotmongkol, Thanyada.
Título: In Silico Screening of DNA Gyrase B Potent Flavonoids for the Treatment of Clostridium difficile Infection from PhytoHub Database
Fonte: Braz. arch. biol. technol;64:e21200402, 2021. tab, graf.
Idioma: en.
Resumo: Abstract Clostridium difficile infection (CDI) is the most common hospital acquired diarrheal disease with its increasing incidence and mortality rate globally. DNA Gyrase B (GyrB) is a key component of DNA replication process across all bacterial genera; thus, this offers a potential target for the treatment of CDI. In the present study, several virtual screening approaches were employed to identify a novel C. difficile GyrB inhibitor. The 139 known metabolites were screened out from the 480 flavonoids in PhytoHub database. Molinspiration and PROTOX II servers were used to calculate the ADME properties and oral toxicity of the metabolites, whereas mutagenicity, tumorigenicity, irritant, and reproductive effect were predicted using DataWarrior program. The binding mode and the binding efficiency of the screened flavonoids against the GyrB were studied using FlexX docking program. From virtual screening of 139 metabolites, we found 25 flavonoids with no mutagenicity, tumorigenicity, irritant, and reproductive effect. Docking study suggested that flavonoids 1030 ((-)-epicatechin 3'-O-sulfate), 1032 ((-)-epicatechin 4'-O-sulfate), 1049 (3'-O-methyl-(-)-epicatechin 4-O-sulfate), 1051 (3'-O-methyl-(-)-epicatechin 7-O-sulfate), 1055 (4'-O-methyl-(-)-epicatechin 7-O-sulfate) and 1317 (quercetin sulfate) have significantly higher binding affinity than the known GyrB inhibitor novobiocin. The results from molecular dynamics simulation and free energy calculations based on solvated interaction energy suggested that (-)-epicatechin 3'-O-sulfate could be a potential drug candidate in the management of CDI.
Descritores: Flavonoides/uso terapêutico
Infecções por Clostridium/terapia
DNA Girase/uso terapêutico
-Ensaios de Triagem em Larga Escala
Responsável: BR1.1 - BIREME


  7 / 359 LILACS  
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Id: biblio-1177420
Autor: Tsai, Wen-Che; Chang, Hung-Chi; Tseng, Yi-Han; Yin, Hsin-Yi; Liao, Jiunn-Wang; Chandra Agrawal, Dinesh; Wen, Hsiao-Wei.
Título: Toxicity evaluation of water extract of tissue-cultured Taraxacum formosanum by acute, subacute administration, and Ames test
Fonte: Electron. j. biotechnol;45:38-45, May 15, 2020. ilus, graf, tab.
Idioma: en.
Projeto: Committee on Chinese Medicine and Pharmacology of the Department of Health, Taiwan.
Resumo: BACKGROUND: Taraxacum species (commonly known as dandelion) used as herbal medicine have been reported to exhibit an antiproliferative effect on hepatoma cells and antitumor activity in non-small-cell lung cancer cells. Although several investigations have demonstrated the safety of Taraxacum officinale, the safety of tissue-cultured plants of T. formosanum has not been assessed so far. Therefore, the present study examines the safety of the water extract of the entire plant of tissue cultured T. formosanum based on acute and subacute toxicity tests in rats, as well as the Ames tests. RESULTS: No death or toxicity symptoms were observed in the acute and subacute tests. The results of the acute test revealed that the LD50 (50% of lethal dose) value of the T. formosanum water extract for rats exceeded 5 g/kg bw. No abnormal changes in the body weight, weekly food consumption, organ weight, or hematological, biochemical, and morphological parameters were observed in the subacute toxicity test. Thus, the no observed adverse effect level (NOAEL) of T. formosanum water extract was estimated to be higher than 2.0 g/kg. Finally, the results of the Ames test revealed that T. formosanum water extract was not genotoxic at any tested concentration to any of five Salmonella strains. CONCLUSIONS: The water extract of tissue-cultured T. formosanum was non-toxic to rats in acute and subacute tests and exhibited no genotoxicity to five Salmonella strains.
Descritores: Extratos Vegetais/toxicidade
Taraxacum/toxicidade
Técnicas de Cultura de Tecidos/métodos
-Segurança
Flavonoides/análise
Cromatografia Líquida de Alta Pressão
Urinálise
Ratos Sprague-Dawley
Fenol/análise
Toxicidade Aguda
Medicina Herbária
Taraxacum/química
Soro
Proliferação de Células/efeitos dos fármacos
Testes de Toxicidade Subaguda
Testes de Mutagenicidade
Limites: Animais
Ratos
Responsável: CL1.1 - Biblioteca Central


  8 / 359 LILACS  
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Texto completo SciELO Chile
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Id: biblio-990001
Autor: Valenzuela-Soto, Ramón; Jiménez-Villarreal, Joel; García-Garza, Rubén; Betancourt-Martínez, Nadia D; Lozoya-Martínez, Rodrigo; Almaráz-Celis, Daniel; Morán-Martínez, Javier.
Título: Evaluación de la actividad antioxidante de Cnidoscolus chayamansa (Chaya), Euphorbia prostrata (Hierba de la Golondrina) y Jatropha dioica (Sangre de Drago) en ratas Wistar inducidas a hiperglicemia / Evaluation of the antioxidant activity of Cnidoscolus chayamansa (Chaya), Euphorbia prostrata (Herb of the Swallow) and Jatropha dioica (Drago blood) in Wistar rats induced to hyperglycemia
Fonte: Int. j. morphol;37(1):36-42, 2019. tab, graf.
Idioma: es.
Resumo: RESUMEN: Estudios recientes han demostrado que los compuestos activos presentes en extractos de C. chayamansa, E. prostrata y J. dioica tienen propiedades antioxidantes. Los resultados obtenidos en nuestro estudio fueron compuestos fenólicos solubles mostraron en C. chayamansa 6,34, E. prostrata 10,67, J. dioica 1,83 mg equiv de ácido gálico/gm BS respectivamente. Los antioxidantes solubles en agua por el método ABTS fueron para C. chayamansa 5.9, E. prostrata 12.7 y para J. dioica 2.5 mM equiv. de trolox/gr BS. Los resultados histopatológicos muestran una mejoría en los tejidos tratados con los extractos después de la inducción a hiperglicemia.

SUMMARY: Recent studies have shown that the active compounds present in extracts of C. chayamansa, E. prostrata and J. dioica have antioxidant properties. The results obtained in our study were soluble phenolic compounds showed in C. chayamansa 6.34, E. prostrata 10.67, J. dioica 1.83 mg equiv of gallic acid/gm BS respectively. The antioxidants soluble in water by the ABTS method were for C. chayamansa 5.9, E. prostrata 12.7 and for J. dioica 2.5 mM equiv. of trolox/gr BS. The histopathological results show an improvement in the tissues treated with the extracts after the induction to hyperglycemia.
Descritores: Extratos Vegetais/administração & dosagem
Euphorbia/química
Jatropha/química
Hiperglicemia/tratamento farmacológico
Antioxidantes/administração & dosagem
-Fenóis/análise
Flavonoides/análise
Extratos Vegetais/química
Ratos Wistar
Compostos Fenólicos
Hiperglicemia/induzido quimicamente
Rim/efeitos dos fármacos
Fígado/efeitos dos fármacos
Antioxidantes/química
Limites: Animais
Ratos
Responsável: CL1.1 - Biblioteca Central


  9 / 359 LILACS  
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Texto completo SciELO Brasil
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Id: biblio-1135509
Autor: Tajrin, Andi; Chandha, Muh Hendra; Mappangara, Surijana; Ruslin, Muhammad; Samad, Rasmidar; Akbar, Fuad Husain.
Título: Analysis of the Total Level of Flavonoids in the Brown Algae ( Phaeophyceae ) Extract as Analgesic and Anti-Inflammatory Drugs
Fonte: Pesqui. bras. odontopediatria clín. integr;20:e4733, 2020. tab, graf.
Idioma: en.
Resumo: Abstract Objective: To determine the total level of flavonoids in brown algae extract Padina sp., Sargassum sp., and Turbinaria sp., which could serve as an analgesic and anti-inflammatory drug. Material and Methods: This is an experimental study with a one-shot case study research design. The study sample consisted of three species of brown algae, namely, Padina sp., Sargassum sp., and Turbinaria sp. The study samples were obtained from Saugi Island, Pangkep, Regency. The sampling method used was convenience sampling. The total flavonoid level in the three extracts of brown algae samples was determined at three concentrations (150 ppm, 300 ppm, and 450 ppm) with three replicates. The analysis used a colorimetric method, a spectrophotometer and aluminium chloride as the reagent. Results: The total level of flavonoids in Padina sp. was the highest at 0.894 ± 0.027%, compared to the levels of 0.786 ± 0.075% in Sargassum sp. and 0.745 ± 0.016% in Turbinaria sp. Conclusion: Padina sp. had the highest total flavonoid levels compared to Sargassum sp. and Turbinaria sp. Flavonoid compounds from brown algae have the potential to be used as analgesic and anti-inflammatory drugs.
Descritores: Alga Marinha
Flavonoides
Feófitas
Compostos Fitoquímicos
Anti-Inflamatórios/uso terapêutico
-Projetos de Pesquisa
Preparações Farmacêuticas
Espectrofotômetros/métodos
Epidemiologia Experimental
Estatísticas não Paramétricas
Sargassum
Indonésia/epidemiologia
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos


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Texto completo SciELO Chile
Texto completo
Id: biblio-950719
Autor: Zia-Ul-Haq, Muhammad; Ahmad, Shakeel; Bukhari, Shazia Anwer; Amarowicz, Ryszard; Ercisli, Sezai; Jaafar, Hawa ZE.
Título: Compositional studies and biological activities of some mash bean (Vigna mungo (L) Hepper) cultivars commonly consumed in Pakistan
Fonte: Biol. Res;47:1-14, 2014. graf, tab.
Idioma: en.
Resumo: BACKGROUND: In recent years, the desire to adopt a healthy diet has drawn attention to legume seeds and food products derived from them. Mash bean is an important legume crop used in Pakistan however a systematic mapping of the chemical composition of mash bean seeds is lacking. Therefore seeds of four mash bean (Vigna mungo (L.) Hepper, family Leguminoseae) cultivars (NARC-Mash-1, NARC-Mash-2, NARC-Mash-3, NARC-Mash-97) commonly consumed in Pakistan have been analyzed for their chemical composition, antioxidant potential and biological activities like inhibition of formation of advanced glycation end products (AGE) activity and tyrosinase inhibition activity. RESULTS: The investigated cultivars varied in terms of biochemical composition to various extents. Mineral composition indicated potassium and zinc in highest and lowest amounts respectively, in all cultivars. The amino acid profile in protein of these cultivars suggested cysteine is present in lowest quantity in all cultivars while fatty acid distribution pattern indicated unsaturated fatty acids as major fatty acids in all cultivars. All cultivars were found to be rich source of tocopherols and sterols. Fourier transform infrared spectroscopy (FTIR) fingerprints of seed flour and extracts indicated major functional groups such as polysaccharides, lipids, amides, amines and amino acids. Results indicated that all investigated cultivars possessed appreciable antioxidant potential. CONCLUSIONS: All cultivars are rich source of protein and possess sufficient content of dietary fiber, a balanced amino acid profile, low saturated fatty acids and antioxidant capacity that rationalizes many traditional uses of seeds of this crop besides its nutritional importance. The collected data will be useful for academic and corporate researchers, nutritionists and clinical dieticians as well as consumers. If proper attention is paid, it may become an important export commodity and may fetch considerable foreign exchange for Pakistan.
Descritores: Sementes/química
Produtos Agrícolas/química
Fabaceae/química
-Paquistão
Fenóis/análise
Riboflavina/análise
Sementes/metabolismo
Esteróis/análise
Taninos/análise
Tiamina/análise
Oligoelementos/análise
Flavonoides/análise
Extratos Vegetais/química
Sequestradores de Radicais Livres
Monofenol Mono-Oxigenase/antagonistas & inibidores
Produtos Finais de Glicação Avançada/metabolismo
Produtos Agrícolas/metabolismo
Substâncias Redutoras/metabolismo
Cisteína/análise
Tocoferóis/análise
Fabaceae/metabolismo
Niacina/análise
Antioxidantes/metabolismo
Valor Nutritivo
Responsável: CL1.1 - Biblioteca Central



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