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  1 / 26 LILACS  
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Id: biblio-1055383
Autor: Yousaf, Abid Mehmood; Malik, Usman Rashid; Shahzad, Yasser; Hussain, Talib; Khan, Ikram Ullah; Din, Fakhar Ud; Mahmood, Tariq; Ahsan, Hafiz Muhammad; Syed, Ahmed Shah; Akram, Muhammad Rouf.
Título: Silymarin-Laden PVP-Nanocontainers Prepared Via the Electrospraying Technique for Improved Aqueous Solubility and Dissolution Rate
Fonte: Braz. arch. biol. technol;62:e19170754, 2019. tab, graf.
Idioma: en.
Resumo: Abstract The aim of the present research was to develop a silymarin-laden PVP-nanocontainer providing ameliorated aqueous solubility and dissolution of the drug. Several silymarin-laden formulations were formed with varying quantities of PVP and SDS via the solvent evaporation method using the electrospraying technique. The influence of the hydrophilic carriers on solubility and dissolution was explored. The solid-state characterization was carried out by particle-size analysis, PXRD, DSC, FTIR and SEM. All of the formulations demonstrated better solubility and dissolution than did silymarin plain powder. Both the SDS and PVP had positive effects on solubility and dissolution of silymarin in the aqueous media. An increased solubility was attained as the drug/PVP ratio was 1/4; however, further increase in PVP did not provide significant improvement. In particular, a nanocontainer formulation prepared with silymarin, PVP and SDS (1/4/0.5, w/w/w) exhibited the best solubility (26432.76 ± 1749.00 μg/mL) and an excellent dissolution (~92 % in 20 min) than did silymarin plain powder. Also, it demonstrated similar dissolution profiles compared to a commercial product; therefore, might be bioequivalent to the commercial product (f 1 = 3 and f 2 = 69). Moreover, cumulative undersize distribution values as represented by X10, X50 and X90 were 201 ± 21.01 nm, 488 ± 36.05 nm and 392 ± 48.10 nm, respectively. The drug existed in the amorphous state in the PVP-nanocontainers with no strong chemical bonding with other excipients. Thus, this formulation might be used for more effective administration of silymarin via the oral route.
Descritores: Silimarina/administração & dosagem
Espectrometria de Massas por Ionização por Electrospray
Dissolução
-Nanopartículas
Responsável: BR1.1 - BIREME


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Id: biblio-843455
Autor: Koçarslan, Aydemir; Koçarslan, Sezen; Aydin, Mehmet Salih; Gunay, Şamil; Karahan, Mahmut Alp; Taşkın, Abdullah; Üstunel, Murat; Aksoy, Nurten.
Título: Intraperitoneal administration of silymarin protects end organs from multivisceral ischemia/reperfusion injury in a rat model
Fonte: Rev. bras. cir. cardiovasc = Braz. j. cardiovasc. surg. (impr.);31(6):434-439, Nov.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract Objective: To determine whether intraperitoneal silymarin administration has favorable effects on the heart, lungs, kidney, and liver and on oxidative stress in a rat model of supraceliac aorta ischemia/reperfusion injury. Methods: Thirty male Wistar albino rats were divided equally into three groups: sham, control, and silymarin. The control and silymarin groups underwent supraceliac aortic occlusion for 45 min, followed by a 60 min period of reperfusion under terminal anesthesia. In the silymarin group, silymarin was administered intraperitoneally during ischemia at a dose of 200 mg/kg. Rats were euthanized using terminal anesthesia, and blood was collected from the inferior vena cava for total antioxidant capacity, total oxidative status, and oxidative stress index measurement. Lungs, heart, liver and kidney tissues were histologically examined. Results: Ischemia/reperfusion injury significantly increased histopathological damage as well as the total oxidative status and oxidative stress index levels in the blood samples. The silymarin group incurred significantly lesser damage to the lungs, liver and kidneys than the control group, while no differences were observed in the myocardium. Furthermore, the silymarin group had significantly lower total oxidative status and oxidative stress index levels than the control group. Conclusion: Intraperitoneal administration of silymarin reduces oxidative stress and protects the liver, kidney, and lungs from acute supraceliac abdominal aorta ischemia/reperfusion injury in the rat model.
Descritores: Aorta Abdominal
Silimarina/administração & dosagem
Traumatismo por Reperfusão/tratamento farmacológico
Estresse Oxidativo
Substâncias Protetoras/administração & dosagem
-Traumatismo por Reperfusão/patologia
Ratos Wistar
Modelos Animais de Doenças
Injeções Intraperitoneais
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


  3 / 26 LILACS  
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Id: biblio-950783
Autor: NK, Asha Tukappa; Londonkar, Ramesh L; Nayaka, Hanumantappa B; CB, Sanjeev Kumar.
Título: Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L
Fonte: Biol. Res;48:1-9, 2015. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: To evaluate the hepatoprotective potential and invitro cytotoxicity studies of whole plant methanol extract of Rumex vesicarius L. Methanol extract at a dose of 100 mg/kg bw and 200 mg/kg bw were assessed for its hepatoprotective potential against CCl4-induced hepatotoxicity by monitoring activity levels of SGOT (Serum glutamic oxaloacetic transaminase), SGPT (Serum glutamic pyruvic transaminase), ALP (Alkaline phosphatase), TP (Total protein), TB (Total bilirubin) and SOD (Superoxide dismutase), CAT (Catalase), MDA (Malondialdehyde). The cytotoxicity of the same extract on HepG2 cell lines were also assessed using MTT assay method at the concentration of 62.5, 125, 250, 500 µg/ml. RESULTS: Pretreatment of animals with whole plant methanol extracts of Rumex vesicarius L. significantly reduced the liver damage and the symptoms of liver injury by restoration of architecture of liver. The biochemical parameters in serum also improved in treated groups compared to the control and standard (silymarin) groups. Histopathological investigation further corroborated these biochemical observations. The cytotoxicity results indicated that the plant extract which were inhibitory to the proliferation of HepG2 cell line with IC50 value of 563.33 ± 0.8 Mg/ml were not cytotoxic and appears to be safe. CONCLUSIONS: Rumex vesicarius L. whole plant methanol extract exhibit hepatoprotective activity. However the cytotoxicity in HepG2 is inexplicable and warrants further study.
Descritores: Extratos Vegetais/farmacologia
Citotoxinas/farmacologia
Rumex/química
Proliferação de Células/efeitos dos fármacos
Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico
Fitoterapia/métodos
-Aspartato Aminotransferases/metabolismo
Silimarina/farmacologia
Superóxido Dismutase/metabolismo
Sais de Tetrazólio
Bilirrubina/metabolismo
Tetracloreto de Carbono
Catalase/metabolismo
Anticarcinógenos/farmacologia
Ratos Wistar
Alanina Transaminase/metabolismo
Metanol
Ingestão de Líquidos/efeitos dos fármacos
Ingestão de Alimentos/efeitos dos fármacos
Fosfatase Alcalina/metabolismo
Células Hep G2
Doença Hepática Induzida por Substâncias e Drogas/metabolismo
Doença Hepática Induzida por Substâncias e Drogas/patologia
Formazans
Fígado/efeitos dos fármacos
Fígado/metabolismo
Malondialdeído/metabolismo
Antioxidantes/metabolismo
Antioxidantes/farmacologia
Limites: Humanos
Animais
Masculino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  4 / 26 LILACS  
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Id: biblio-1134510
Autor: Salman, Ahmed; El-Ghazouly, Dalia El-Sayed; El-Beltagy, Maha.
Título: Role of ascorbic acid versus silymarin in amelioration of hepatotoxicity induced by acrylamide in adult male albino rats: histological and immunohistochemical study / Rol del ácido ascórbico frente a la silimarina en la mejoría de la hepatotoxicidad inducida por acrilamida en ratas albinas macho adultas: estudio histológico e inmunohistoquímico
Fonte: Int. j. morphol;38(6):1767-1778, Dec. 2020. tab, graf.
Idioma: en.
Resumo: SUMMARY: Acrylamide (ACR) is a cytotoxic and carcinogenic material. It is a product of a Maillard reaction during the cooking of many types of fried fast food, e.g. potato chip fries, and chicken nuggets. ACR has a severe toxic effect on different body organs. This study investigates the hepatotoxic effect of ACR, and the protective effect of ascorbic acid and silymarin. For this purpose, forty adult, male, albino rats were divided into four groups and received the following treatments for fourteen days: Group I: (the control) normal saline; Group II: ACR only; Group III: ACR and ascorbic acid; and Group IV: ACR and silymarin. Under a light microscope, the liver from rats treated with ACR only presented disturbed liver architecture, degenerated hepatocytes, reduced glycogen contents, congested central vein, and increased collagen fibres with areas of fibrosis. Immunohistochemical examination revealed an increased mean number of CD68-, and α-SMA-positive cells. This indicates the presence of large numbers of stellate macrophages (Kupffer cells) and Hepatic stellate cells (HSCs). The combination of ACR with either ascorbic acid or silymarin resulted in less hepatic degeneration, less fibrosis and fewer CD68 and α-SMA positive cells compared to the ACR only group. In conclusion, treatment with silymarin or ascorbic acid along with ACR appears to alleviate ACR-induced hepatotoxicity with more protection in silymarin treated rats.

RESUMEN: La acrilamida (ACR) es un material citotóxico y cancerígeno. Es producto de la reacción de Maillard durante la cocción de muchos tipos de comida rápida y frita, por ejemplo: papas fritas y nuggets de pollo. ACR tiene un efecto tóxico severo en diferentes órganos del cuerpo. Este estudio investigó el efecto hepatotóxico del ACR y el efecto protector del ácido ascórbico y la silimarina. Con este fin, cuarenta ratas albinas machos adultas se dividieron en cuatro grupos y recibieron los siguientes tratamientos durante catorce días: Grupo I (control), solución salina normal; Grupo II, solo ACR; Grupo III, ACR y ácido ascórbico; y Grupo IV, ACR y silimarina. Bajo microscopio óptico, el hígado de ratas tratadas con ACR solo presentó alteración de su arquitectura, entre ellos hepatocitos degenerados, contenido reducido de glucógeno, vena central congestionada y aumento de fibras de colágeno con áreas de fibrosis. El examen inmunohistoquímico reveló un aumento del número medio de células CD68 y α-SMA positivas. Esto indica la presencia de un gran número de macrófagos estrellados (células de Kupffer) y células estrelladas hepáticas (HSC). La combinación de ACR con ácido ascórbico o silimarina resultó en menos degeneración hepática, menos fibrosis y menos células positivas para CD68 y α-SMA en comparación con el grupo de ACR solo. En conclusión, el tratamiento con silimarina o ácido ascórbico junto con ACR parece aliviar la hepatotoxicidad inducida por ACR.
Descritores: Ácido Ascórbico/farmacologia
Silimarina/farmacologia
Acrilamida/toxicidade
Fígado/efeitos dos fármacos
-Imuno-Histoquímica
Antígenos CD/análise
Actinas/análise
Hepatócitos
Células Estreladas do Fígado
Fígado/metabolismo
Fígado/patologia
Limites: Animais
Masculino
Ratos
Responsável: CL1.1 - Biblioteca Central


  5 / 26 LILACS  
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Id: biblio-1131497
Autor: Amaral, A. V. C; Parente, L. M. L; Conceição, E. C; Paula, J. R; Cunha, L. C; Costa, A. P. A; Oliveira, L. S; Benatti, L. A. T; Fioravanti, M. C. S.
Título: Bidens pilosa L. (Asteraceae) cultivated in Brazil on acute liver disease in dogs / [Bidens pilosa L. (Asteraceae) cultivada no Brasil na doença hepática aguda em cães]
Fonte: Arq. bras. med. vet. zootec. (Online);72(4):1248-1257, July-Aug. 2020. tab, ilus.
Idioma: en.
Resumo: Bidens pilosa L. is a medicinal plant popularly used for treatment of liver diseases. In this study, the dry extract of aerial parts of Bidens pilosa and Silymarin, a phytocomplex obtained from the Silybum marianum fruits and marketed as hepatoprotective, were tested in dogs experimentally acutely intoxicated with carbon tetrachloride. The liver activity was evaluated by hematological and biochemical profiles, and histological and ultrasound analyzes. It was observed that the lowest serum activities of ALT and serum concentrations of total bilirubin occurred in the groups treated with the dry extract of Bidens pilosa, while only decreased serum concentrations of total bilirubin occurred in the group treated with Silymarin. Best liver recovery was also observed for the dry extract of B. pilosa at a 400mg/Kg dose by ultrasonography. This study showed that the dry extract of Bidens pilosa acted more efficiently in the treatment of acute toxic hepatitis induced in dogs than Silymarin.(AU)

Bidens pilosa L. é uma planta medicinal utilizada popularmente para tratamento de doenças hepáticas. Neste trabalho, o extrato seco das partes aéreas da Bidens pilosa e a silimarina, um fitocomplexo obtido dos frutos da Silybum marianum e comercializado como hepatoprotetor, foram testados em cães intoxicados experimentalmente de forma aguda com tetracloreto de carbono. A atividade hepática foi avaliada por meio dos perfis hematológico e bioquímico, análises histológica e ultrassonográfica. Observou-se que, nos grupos tratados com o extrato seco da Bidens pilosa, ocorreram as menores atividades séricas da ALT e de concentrações séricas de bilirrubina total, enquanto no grupo tratado com silimarina, ocorreu apenas diminuição de concentrações séricas de bilirrubina total. Melhor recuperação hepática também foi verificada para o extrato seco de B. pilosa na dose de 400mg/kg por ultrassonografia. Este estudo evidenciou que o extrato seco da Bidens pilosa atuou de forma mais eficiente no tratamento da hepatite aguda tóxica induzida em cães do que a silimarina.(AU)
Descritores: Intoxicação por Tetracloreto de Carbono/terapia
Intoxicação por Tetracloreto de Carbono/veterinária
Bidens/química
Hepatite Animal/terapia
-Plantas Medicinais
Silimarina/uso terapêutico
Limites: Animais
Cães
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


  6 / 26 LILACS  
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Martínez, Jaime
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Id: lil-668303
Autor: Arroyo, Jorge; Almora, Yuan; Quino, Mariano; Raez, Ernesto; Martínez, Jaime; Buendía, Jesús; Baca, Deybis; Hañari, Renan.
Título: Efecto protector en cirrosis hepática inducida en ratas del extracto etanólico de las hojas de Piper aduncum comparado con silimarina / Piper aduncum leaves ethanol extracts protective effect compared with silymarin in liver cirrhosis induced in rats
Fonte: An. Fac. Med. (Perú);73(2):85-91, abr.-jun. 2012. ilus, tab, graf.
Idioma: es.
Resumo: Objetivos: Evaluar la eficacia protectora del extracto etanólico de hojas de Piper aduncum (matico) y su fitomedicamento en cápsulas, en la cirrosis hepática inducida en ratas. Diseño: Experimental. Lugar: Facultad Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú. Material biológico: Hojas de Piper aduncum, y Rattus norvegicus, cepa Holtzman. Intervenciones: Las hojas fueron recolectadas en el distrito de Huariaca, departamento de Pasco. El fitomedicamento en cápsulas se preparó a partir del extracto etanólico de la planta. La cirrosis fue inducida con fenobarbital 0,5 mg/mL, diluida en el agua de beber por 15 días, y luego, tetracloruro de carbono 0,2mL/kg en aceite de oliva 1:1, oralmente por 7 días. Se colectó una muestra de sangre para determinar perfil hepático y malondialdehído; los animales fueron sacrificados extrayéndose el hígado para estudio histopatológico. Los datos fueron evaluados mediante técnicas multivariadas, con valor p < 0,05. Principales medidas de resultados: Grado de lesión hepática, marcadores bioquímicos, estrés oxidativo. Resultados: El extracto y el fitomedicamento a 200 mg/kg disminuyeron los valores de TGP (p < 0,621), bilirrubina total (p < 0,385) y bilirrubina directa (p < 0,283) e incrementaron las proteínas totales (p < 0,539) y albúmina (p < 0,114), similar al grupo silimarina. El colágeno, la fibrosis y el nivel de daño hepático se vieron aumentados con tetracloruro de carbono; estos indicadores se redujeron con los diferentes tratamientos y la silimarina. El marcador de estrés oxidativo se redujo con los tratamientos aplicados (p < 0,002). Conclusiones: El extracto etanólico de las hojas de Piper aduncum (matico) y su fitomedicamento ejercieron efecto protector de la cirrosis inducida en ratas, comparativamente con la silimarina.

Objectives: To determine the protective effect of Piper aduncum (matico) leaves ethanol extract and its phytomedicine in capsules in liver cirrhosis induced in rats. Design: Experimental. Location: Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biological material: Leaves of Piper aduncum, and Rattus norvegicus, Holtzman strain. Interventions: The leaves were collected in La Merced district, Pasco department. The phytomedicine capsules were prepared from the plant ethanol extract. Cirrhosis was induced with phenobarbital 0.5 mg/mL diluted in drinking water during 15 days, then carbon tetrachloride 0.2mL/kg 1:1 in olive oil orally during 7 days. Blood samples were drawn in order to determine liver profile and malondialdehyde; the animals were sacrificed, extracting the liver for pathology study. Data were evaluated by multivariate techniques with p <0.05. Main outcome measures: Degree of liver injury, biochemical marks, oxidative stress. Results: About 200 mg/kg of both extract and phytomedicine decreased ALT values (p < 0.621), total bilirrubin (p < 0.385) and direct bilirrubin (p < 0.283) and increased total protein (p <0.539) and albumin (p <0.114), similar to silymarin group. Collagen, fibrosis and liver damage degree increased with carbon tetrachloride, and reduced with the different treatments and silymarin. Oxidative stress marker was also reduced with the applied treatments (p <0.002). Conclusions: Piper aduncum (matico) leaves ethanol extract and its phytomedicine had protective effect of cirrhosis induced in rats similar to silymarin.
Descritores: Cirrose Hepática Experimental
Extratos Vegetais
Matico
Silimarina
-Epidemiologia Experimental
Limites: Masculino
Animais
Ratos
Responsável: PE13.1 - Oficina de Biblioteca, Hemeroteca y Centro de Documentación


  7 / 26 LILACS  
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Id: biblio-886210
Autor: Özer, Nazmi; Bülbüloğlu, Ertan; Yormaz, Serdar; Ece, İlhan.
Título: Evaluation of silybum marinaum efficacy on University of Wisconsin and histidine-tryptophan-ketoglutarate solutions latter the damage of the perfused liver
Fonte: Acta cir. bras;32(6):407-417, June 2017. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To investigate the hepatoprotective and antioxidant effeicacies of Silybum marianum's (silymarin, S) on University of Wisconsin (UW) and histidinetryptophan-ketoglutarate (HTK) preservation solutions. Methods: Thirty two Wistar albino adult male rats were used. Group 1: UW group, Group 2: UW + Silymarin group(S), Group 3: HTK group, Group 4: HTK + silymarin group (S), respectively. Silymarin was enforced intraperitoneally before the surgery. Biopsies were enforced in 0, 6 and 12.hours to investigate. Results: Biochemical parameters examined in alanine aminotransferase (ALT), furthermore superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in rats were also evaluated. Detected histopathological changings were substantially declining in the groups that received silymarin, cellular damage was decreased significantly in HTK + Silymarin group, according to other groups. It has been identified as the most effective group was HTK + silymarin group in evaluation of ALT, electron microscopic results, also decreased MDA and elevated in SOD, and CAT activity. Caspase 3 analysis showed a substantial lower apoptosis ratio in the silymarin groups than in the non-performed groups (p<0.05). Conclusion: Histidinetryptophan-ketoglutarate+silymarin group provides better hepatoprotection than other groups, by decreasing the hepatic pathologic damage, delayed changes that arise under cold ischemic terms.
Descritores: Silimarina/uso terapêutico
Soluções para Preservação de Órgãos
Substâncias Protetoras/uso terapêutico
Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico
Antioxidantes/uso terapêutico
-Cloreto de Potássio
Procaína
Rafinose
Imuno-Histoquímica
Adenosina
Alopurinol
Ratos Wistar
Modelos Animais de Doenças
Glucose
Glutationa
Insulina
Manitol
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


  8 / 26 LILACS  
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Id: biblio-837659
Autor: Onalan, Ali Kemal; Tuncal, Salih; Kilicoglu, Sibel; Celepli, Salih; Durak, Esra; Kilicoglu, Bulent; Devrim, Erdinc; Barlas, Aziz Mutlu; Kismet, Kemal.
Título: Effect of silymarin on oxidative stress and liver histopathology in experimental obstructive jaundice model
Fonte: Acta cir. bras;31(12):801-806, Dec. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT PURPOSE: To investigate the effect of silymarin on oxidative stress and hepatic injury induced by obstructive jaundice in an experimental model. METHODS: Thirty Wistar-Albino type female rats were divided into 3 groups each including 10 rats. Only laparotomy was performed in group 1. Bile duct ligation was performed in group 2. In group 3, bile duct ligation was performed and orogastic silymarin 300 mg/kg/day dose was given for seven days. At the end of seven days, rats were sacrificed. The blood and liver tissue samples were taken to be examined biochemically and histopathologically. RESULTS: The plasma and liver levels of malondialdehyde were significantly lower in silymarin group than in the bile duct ligated group. Although liver levels of GSH were significantly higher in silymarin group than in the bile duct ligated group, there was no significant difference between the plasma GSH levels of these groups. In silymarin group; the enlargement of hepatocytes, dilatation of canaliculi and the edema were regressed. CONCLUSION: Silymarin diminished the harmful effects of obstructive jaundice on liver.
Descritores: Silimarina/farmacologia
Estresse Oxidativo/efeitos dos fármacos
Icterícia Obstrutiva/complicações
Fígado/patologia
Antioxidantes/farmacologia
-Ductos Biliares
Distribuição Aleatória
Ratos Wistar
Substâncias Protetoras/farmacologia
Icterícia Obstrutiva/patologia
Glutationa/sangue
Ligadura
Malondialdeído/sangue
Limites: Animais
Feminino
Ratos
Responsável: BR1.1 - BIREME


  9 / 26 LILACS  
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Id: lil-781326
Autor: Ergün, Yusuf; Üremiş, Muhammed; Kılınç, Metin; Alıcı, Tuğrul.
Título: Antioxidant effect of Legalon(r) SIL in ischemia-reperfusion injury of rat skeletal muscle
Fonte: Acta cir. bras;31(4):264-270, Apr. 2016. tab, graf.
Idioma: en.
Resumo: PURPOSE: To evaluated the potential antioxidant agent Legalon (r) SIL (silibinin-C-2',3-bis(hydrogensuccinat)) in the skeletal muscle of rats. METHODS: IRI was achieved via tourniquet application in Wistar-albino rats. Experimental groups were chosen as (i) sham control, (ii) IRI (3+2 h), (iii) IRI and Legalon (r) SIL-50 (50 mg/kg/i.p.), (iv) IRI and Legalon (r) SIL-100 (100 mg/kg/i.p.), and (v) IRI and Legalon (r) SIL-200 (200 mg/kg/ i.p.). Muscle viability (evaluated by triphenyltetrazolium chloride dye method), malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase were assessed in muscle samples using a spectrophotometer. RESULTS: Although viability of the injured limb non-significantly declined in the IRI group, administration of Legalon (r) SIL did not prevent injury. However, dramatic increase observed in malondialdehyde levels in the IRI group was prohibited by Legalon (r) SIL in a statistically significant manner. In comparison with the sham-control group, IRI and Legalon (r) SIL administration did not cause any significant alterations in the levels of superoxide dismutase, catalase, and glutathione peroxidase. CONCLUSION: Although Legalon (r) SIL was not sufficient to prevent muscle injury in terms of viability, it is found to be an effective option to reduce reactive oxygen species-induced cell injury.
Descritores: Silimarina/farmacologia
Traumatismo por Reperfusão/prevenção & controle
Músculo Esquelético/irrigação sanguínea
Isquemia/prevenção & controle
Antioxidantes/farmacologia
-Valores de Referência
Superóxido Dismutase/análise
Superóxido Dismutase/efeitos dos fármacos
Sobrevivência de Tecidos/efeitos dos fármacos
Catalase/análise
Catalase/efeitos dos fármacos
Distribuição Aleatória
Reprodutibilidade dos Testes
Espécies Reativas de Oxigênio/análise
Ratos Wistar
Estresse Oxidativo/efeitos dos fármacos
Músculo Esquelético/efeitos dos fármacos
Músculo Esquelético/química
Glutationa Peroxidase/análise
Glutationa Peroxidase/efeitos dos fármacos
Malondialdeído/análise
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  10 / 26 LILACS  
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Id: lil-623946
Autor: Rui, Yao-Cheng.
Título: Advances in pharmacological studies of silymarin
Fonte: Mem. Inst. Oswaldo Cruz;86(supl.2):79-85, 1991. ilus, tab, graf.
Idioma: en.
Conferência: Apresentado em: Brazilian-Sino Symposium on Chemistry and Pharmacology of Natural Products, Rio de Janeiro, Dec. 10-14, 1989.
Resumo: Silymarin is the flavonoids extracted from the seeds of Silybum marianum (L) Gearth as a mixture of three structural isomers: silybin, silydianin and silychristin, the former being the most active component. Silymarin protects liver cell membrane against hepatotoxic agents and improves liver function in experimental animals and humans. It is generally accepted that silymarin exerts a membrane-stabilizing action preventing or inhibiting membrane peroxidation. The experiments with soybean lipoxygenase showed that the three components of silymarin brought about a concentration-dependent non-competitive inhibition of the lipoxygenase. The experiments also showed an analogous interaction with animal lipoxygenase, thus showing that an inhibition of the peroxidation of the fatty acid in vivo was self-evident. Silybin almost completely suppressed the formation of PG at the highest concentration (0.3 mM) and proved to be an inhibitor of PG synthesis in vitro. In our experiments, silybin at lower dose (65 mg/Kg) decreased liver lipoperoxide content and microsomal lipoperoxidation to 84.5% and 68.55% of those of the scalded control rats respectively, and prevented the decrease of liver microsomal cytochrome p-450 content and p-nitroanisole-0-demethylase activity 24 h post-scalding. Effects of silymarin on cardiovascular systen have been studied in this university since 1980. O. O silymarin 800 mg/Kg/d or silybin 600 mg/Kg/d reduced plasma total cholesterol, LDL-C and VLDL-C. They however, enhanced HDL-C in hyperlipenic rats. Further studies showed that silymarin enhanced HDL-C in hyperlipemic rats. Further studies showed that silymarin enhanced HDL-C but didn't affect HDL-C, a property of this component which is beneficial to treatment of atherosclerosis. The results showed silymarin 80 mg or silybin 60 mg decreased in vitro platelet aggregation (porcentagem) in rats. The maximal platelet aggregation induced by ADP declined significantly, and time to reach maximal platelet aggregation and five-minute disaggregation didn't change. In our experiments, iv silybin 22,4 mg/kg lowered the amplitude and duration of diastolic blood pressure (DBP) more than those of systolic (SBP), but the descending aortic blood flow, cardiac contractility and ECG did not change significantly in anesthetized open-chest cats. The results indicated a reduction of peripheral resistance and dilatatory action on the resistant blood vessels. These effects are beneficial to coronary heart disease. We also observed the effects of silybin on morphological change, the release of glutamic oxaloacetate aminotrasferase (GOT) and lactate dehydrogenase (LDH) as well as the radioactivity of 3H-TdR incorporated into DNA in normal cardiac cells and cells infected by coxsackie B5, virus os newborn rats. The results showed that silynin did not affect the morphology of normal cell, and that the pathological change of cells infected by virus was delayed and reduced as compared to control. We have investigated the effect of silybin on synthesis and release of LTs in the cultured porcine cerebral basilar arteries (PCBA). Silybin 100 and 500 µmol/L declined the amounts of LTs released from the PCBA incubsated in the presence of A 23187, AA and indomenthacin. The result suggests that silybin can inhibit the activity of 5-lipoxygenase of cerebral blood vessel and may protect the brain from ischemia.
Descritores: Silimarina/uso terapêutico
Inibidores da Agregação Plaquetária/farmacologia
Sistema Cardiovascular/efeitos dos fármacos
Inibidores de Lipoxigenase/farmacologia
Hemodinâmica/efeitos dos fármacos
Antioxidantes/farmacologia
-Lipídeos/sangue
Fígado
Limites: Animais
Responsável: BR1.1 - BIREME



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