Base de dados : LILACS
Pesquisa : D03.383.663.718 [Categoria DeCS]
Referências encontradas : 5 [refinar]
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Texto completo SciELO Brasil
Texto completo
Id: biblio-974297
Autor: Shakibaie, Mojtaba; Ameri, Atefeh; Ghazanfarian, Roya; Adeli-Sardou, Mahboubeh; Amirpour-Rostami, Sahar; Torkzadeh-Mahani, Masoud; Imani, Mehdi; Forootanfar, Hamid.
Título: Statistical optimization of kojic acid production by a UV-induced mutant strain of Aspergillus terreus
Fonte: Braz. j. microbiol;49(4):865-871, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Kerman University of Medical Sciences; . Elite Researcher Grant Committee.
Resumo: ABSTRACT The ability of four Aspergillus strains for biosynthesis of kojic acid was evaluated among which Aspergillus terreus represented the highest level (2.21 g/L) of kojic acid production. Improvement kojic acid production ability of A. terreus by random mutagenesis using different exposure time to ultraviolet light (5-40 min) was then performed to obtain a suitable mutant of kojic acid production (designated as C5-10, 7.63 g/L). Thereafter, design of experiment protocol was employed to find medium components (glucose, yeast extract, KH2PO4 (NH4)2SO4, and pH) influences on kojic acid production by the C5-10 mutant. A 25-1 fractional factorial design augmented to central composite design showed that glucose, yeast extract, and KH2PO4 were the most considerable factors within the tested levels (p < 0.05). The optimum medium composition for the kojic acid production by the C5-10 mutant was found to be glucose, 98.4 g/L; yeast extract, 1.0 g/L; and KH2PO4, 10.3 mM which was theoretically able to produce 120.2 g/L of kojic acid based on the obtained response surface model for medium optimization. Using these medium compositions an experimental maximum Kojic acid production (109.0 ± 10 g/L) was acquired which verified the efficiency of the applied method.
Descritores: Pironas/metabolismo
Aspergillus/efeitos da radiação
Aspergillus/metabolismo
-Aspergillus/crescimento & desenvolvimento
Aspergillus/genética
Raios Ultravioleta
Mutagênese
Meios de Cultura/metabolismo
Fermentação
Glucose/metabolismo
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: biblio-840307
Autor: Luo, Haiyu; Xu, Dan; Xie, Rushan; Zhang, Xuping; Wang, Jian; Xuejiao, Dong; Lai, Daowan; Zhou, Ligang; Liu, Yang.
Título: Enhancement of botrallin and TMC-264 production in liquid culture of endophytic fungus Hyalodendriella sp. Ponipodef12 after treatments with metal ions
Fonte: Electron. j. biotechnol;19(6):12-20, Nov. 2016. ilus.
Idioma: en.
Projeto: Hi-Tech R & D Program of China; . National Basic Research Program of China.
Resumo: Background: Hyalodendriella sp. Ponipodef12, an endophytic fungus from a poplar hybrid, was a high producer of botrallin and TMC-264 with various bioactivities. In this study, the influences of eight metal ions (i.e.,Mn2+,Na+, Mg2+,Zn2+,Cu2+,Fe2+,Fe3+ and Al3+) on botrallin and TMC-264 production in liquid culture of the endophytic fungus Hyalodendriella sp. Ponipodef12 were investigated. Results: Three most effective metal ions (Zn2+,Cu2+ and Mg2+) along with their optimum concentrations were screened. The optimum addition time and concentrations of Zn2+,Cu2+ and Mg2+ were further obtained respectively for improving botrallin and TMC-264 production. The combination effects of Zn2+,Cu2+ and Mg2+ on the production of botrallin and TMC-264 by employing statistical method based on the central composite design (CCD) and response surface methodology (RSM) were evaluated, and two quadratic predictive models were developed for botrallin and TMC-264 production. The yields of botrallin and TMC-264, which were predicted as 144.12 mg/L and 36.04 mg/L respectively, were validated to be 146.51 mg/L and 36.63 mg/L accordingly with the optimum concentrations of Zn2+ at 0.81 mmol/L, Cu2+ at 0.20 mmol/L, and Mg2+ at 0.13 mmol/L in medium. Conclusion: The results indicated that the enhancement of botrallin and TMC-264 accumulation in liquid culture of the endophytic fungus Hyalodendriella sp. Ponipodef12 by the metal ions and their combination should be an effective strategy.
Descritores: Ascomicetos/metabolismo
Compostos Heterocíclicos com 3 Anéis/metabolismo
Pironas/metabolismo
-Ascomicetos/efeitos dos fármacos
Compostos Heterocíclicos com 3 Anéis/química
Metais/farmacologia
Pironas/química
Responsável: CL1.1 - Biblioteca Central


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Leite, Selma Gomes Ferreira
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Id: lil-504329
Autor: Ramos, Aline de Souza; Fiaux, Sorele Batista; Leite, Selma Gomes Ferreira.
Título: Production of 6-pentyl-alfa-pyrone by trichoderma harzianum in solid-state fermentation / Produção de 6-pentil-alfa-pirona por Trichoderma harzianum em fermentação em estado sólido
Fonte: Braz. j. microbiol;39(4):712-717, Dec. 2008. graf, tab.
Idioma: en.
Resumo: Many Trichoderma species are able to produce 6-pentyl-α-pyrone (6-PP), a lactone with coconut-like aroma. In the present work, several culture parameters were studied to enhance the production of 6-PP by Trichoderma harzianum 4040 in solid-state fermentation. Green coir powder added to a nutrient solution was used as support material for fermentation. A Plackett-Burman screening technique was applied, followed by a fractionary factorial design. The best culture conditions within the experimental domain studied were (100 g support)-1: sucrose, 3 g; NaNO3, 0.24 g; (NH4)2SO4, 0.18 g; KH2PO4, 0.1 g; inoculum concentration, 2.2 x 10(6) spores; moisture level, 55 percent. The temperature established was 28ºC. The fermentation under the selected conditions led to a 6-PP production six times higher (5.0 mg/g dry matter) than the initial one (0.8 mg/g dry matter) after seven days of cultivation.

Muitas espécies do gênero Trichoderma são capazes de produzir a substância 6-pentil-α-pirona (6-PP), uma lactona com aroma característico de coco. No presente trabalho, vários parâmetros de cultura foram estudados para aumentar a produção de 6-PP por Trichoderma harzianum 4040 em fermentação em estado sólido. Pó da casca de coco verde adicionado à uma solução nutriente foi usado como material de suporte para a fermentação. Um planejamento experimental de varredura segundo a técnica de Plackett-Burman foi aplicado, seguido de um planejamento fatorial fracionário. No domínio experimental estudado, as melhores condições de cultura foram (100 g suporte)-1: sacarose, 3 g; NaNO3, 0,24 g; (NH4)2SO4, 0,18 g; KH2PO4, 0,1 g; produção do inóculo, 2,2 x 10(6) esporos; umidade, 55 por cento. A temperatura estabelecida foi de 28ºC. Esse estudo conduziu à concentração de 6-PP seis vezes maior (5,0 mg/g de matéria seca) do que a inicial (0,8 mg/g de matéria seca) após sete dias de cultivo.
Descritores: Fermentação
Microbiologia Industrial
Nitrogênio
Pironas
Trichoderma
-Cromatografia Gasosa
Métodos
Técnicas
Tipo de Publ: Relatório Técnico
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Tedesco, A. C
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Id: lil-303316
Autor: Gomes, A. J; Lunardi, C. N; Gonzalez, S; Tedesco, A. C.
Título: The antioxidant action of Polypodium leucotomos extract and kojic acid: reactions with reactive oxygen species
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;34(11):1487-1494, Nov. 2001. ilus, tab.
Idioma: en.
Resumo: Two natural products Polypodium leucotomos extract (PL) and kojic acid (KA) were tested for their ability to scavenge reactive oxygen species (ÀOH, ÀO2-, H2O2, ¹O2) in phosphate buffer. Hydroxyl radicals were generated by the Fenton reaction, and the rate constants of scavenging were 1.6 x 10(9) M-1 s-1 for KA and 1.0 x 10(9) M-1 s-1 for PL, similar to that of ethanol (1.4 x 10(9) M-1 s-1). With superoxide anions generated by the xanthine/hypoxanthine system, KA and PL (0.2-1.0 mg/ml) inhibited ÀO2-dependent reduction of nitroblue tetrazolium by up to 30 and 31 percent, respectively. In the detection of ¹O2 by rose bengal irradiation, PL at 1.0 mg/ml quenched singlet oxygen by 43 percent relative to azide and KA by 36 percent. The present study demonstrates that PL showed an antioxidant effect, scavenging three of four reactive oxygen species tested here. Unlike KA, PL did not significantly scavenge hydrogen peroxide
Descritores: Antioxidantes
Espécies Reativas de Oxigênio
Depuradores de Radicais Livres
Pironas
-Extratos Vegetais
Tampões Cirúrgicos
Responsável: BR1.1 - BIREME


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Id: lil-286196
Autor: Buitrón García Figueroa, Rafael; Sauceda, Luciano; Ramírez Palacios, Diógenes; Cruz Talonia, Fernando; Romero Cabello, Raúl.
Título: Eficacia y seguridad de ciclopirox olamina en crema vaginal al 1 por ciento contra terconazol en crema vaginal al 0.8 por ciento en el tratamiento de candidiasis genital / Ciclopirox olamine 1 percent cream versus terconazole 0.8 percent cream effectiveness and safety in genital candidiasis treatment
Fonte: Ginecol. obstet. Méx;68(4):154-9, abr. 2000. tab.
Idioma: es.
Resumo: Se efectuó un estudio multicéntrico comparativo, aleatorio, seleccionando 170 pacientes de 18 años o más, con diagnóstico clínico de candidiasis genital y con cultivo positivo a Candida, los cuales se asignaron aleatoriamente al tratamiento con ciclopirox olamina en crema vaginal al 1 por ciento (85 pacientes) o con terconazol en crema vaginal al 0.8 por ciento (85 pacientes) durante seis días, con el objeto de comparar la eficacia clínica, la eficacia fungicida y la seguridad de ambos tratamientos. Los resultados de la eficacia mixta (clínica y micológica) para ciclopirox olamina fue de cura al final del tratamiento - día siete - en 48 pacientes (62.3 por ciento) y al seguimiento - día 21 - de 42 (55.30 por ciento) y la mejoría al final en 25 pacientes (32.5 por ciento) y en el seguimiento 21 (27.6 por ciento) y para terconazol fue de cura al final en 45 pacientes (61.6 por ciento) y al seguimiento en 39 (57.4 por ciento) y de mejoría al final en 23 (31.5 por ciento) y en el seguimiento, 22 (32.4 por ciento). Se concluye que ambos medicamentos son eficaces y seguros para el tratamiento de candidiasis genital.
Descritores: Candidíase Vulvovaginal/tratamento farmacológico
Pironas/uso terapêutico
Triazóis/uso terapêutico
-Antifúngicos/uso terapêutico
Candida albicans/patogenicidade
Micoses/tratamento farmacológico
Limites: Seres Humanos
Feminino
Adolescente
Adulto
Responsável: MX1.1 - CENIDSP - Centro de Información para Decisiones en Salud Pública



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