Base de dados : LILACS
Pesquisa : D03.633.100.473.231.370 [Categoria DeCS]
Referências encontradas : 9 [refinar]
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Id: biblio-1290644
Autor: Feitosa, André de O; Dias, Amanda Cristina S; Ramos, Gisele da C; Bitencourt, Heriberto R; Siqueira, José Edson S; Marinho, Patrícia Santana B; Barison, Andersson; Ocampos, Fernanda M. M; Marinho, Adrey Moacir do R.
Título: Letalidad de citocalasina B y otros compuestos aislados del hongo Aspergillus spp. (Trichocomaceae) endófito de Bauhinia guianensis (Fabaceae) / Lethality of cytochalasin B and other compounds isolated from fungus Aspergillus sp. (Trichocomaceae) endophyte of Bauhinia guianensis (Fabaceae)
Fonte: Rev. argent. microbiol;48(3):259-263, set. 2016. ilus..
Idioma: en.
Resumo: Los hongos endofíticos son hongos que colonizan los tejidos internos de las plantas; varios compuestos biológicamente activos se han aislado a partir de estos hongos. Existen pocos estudios de compuestos aislados de hongos endófitos de plantas amazónicas. Por lo tanto, este estudio tuvo como objetivo el aislamiento y la identificación estructural de ergosterol (1), peróxido de ergosterol (2), mevalonolactona (3), citocalasina B (4) y citocalasina H (5) a partir de Aspergillus spp. EJC 04, un hongo endofítico de Bauhinia guianensis. La citocalasina B (4) y el derivado diacetato de citocalasina B (4a) mostraron una alta letalidad en el ensayo de Artemia salina. Esta es la primera aparición de citocalasinas en hongos endófitos amazónica de B. guianensis

Endophytic fungi are fungi that colonize internal tissues of plants; several biologically active compounds have been isolated from these fungi. There are few studies of compounds isolated from endophytic fungi of Amazon plants. Thus, this study aimed the isolation and structural identification of ergosterol (1), ergosterol peroxide (2), mevalonolactone (3), cytochalasin B (4) and cytochalasin H (5) from Aspergillus sp. EJC 04, an endophytic fungus from Bauhinia guianensis. The cytochalasin B (4) and the diacetate derivative of cytochalasin B (4a) showed high lethality in the brine shrimp assay. This is the first occurrence of cytochalasins in Amazonian endophytic fungi from B. guianensis
Descritores: Artemia/efeitos dos fármacos
Aspergillus/imunologia
Citocalasina B/isolamento & purificação
Citocalasina B/análise
Citocalasinas/isolamento & purificação
Bauhinia/microbiologia
Ergosterol/isolamento & purificação
Endófitos/patogenicidade
Tipo de Publ: Estudo de Avaliação
Estudo Observacional
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


  2 / 9 LILACS  
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Id: lil-595004
Autor: Bevacqua, Romina J; Fernandez-Martin, Rafael; Salamone, Daniel F.
Título: Bovine parthenogenotes produced by inhibition of first or second polar bodies emission
Fonte: Biocell;35(1):1-7, Apr. 2011. ilus, tab, graf.
Idioma: en.
Resumo: Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid chromosome set. In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. Most oocytes (77%) were arrested at a meiotic stage characterized by the presence of a large internal metaphase plate and absence of polar body. In experiment 2, development of embryos exposed to cytochalasin B during in vitro maturation (CytoB-IVM) or during activation (CytoB-ACT) was compared. Developmental rates did not differ between diploidization strategies, even when three agents were employed to induce activation. Both groups, CytoB-IVM and CytoB-ACT, tended to maintain diploidy. CytoB-IVM parthenogenesis could help to obtain embryos with a higher degree of homology to the oocyte donor.
Descritores: Citocalasina B/farmacologia
Embrião de Mamíferos/citologia
Embrião de Mamíferos
Embrião de Mamíferos/fisiologia
Meiose
Oócitos/citologia
Oócitos
Oócitos/metabolismo
-Partenogênese
Ploidias
Limites: Humanos
Bovinos
Animais
Feminino
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo


  3 / 9 LILACS  
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Texto completo SciELO Brasil
Texto completo
Id: lil-512771
Autor: Feng, D. Q; Zhou, Y; Ling, B; Gao, T; Shi, Y. Y; Wei, H. M; Tian, Z. G.
Título: Effects of the conditioned medium of mesenchymal stem cells on mouse oocyte activation and development
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;42(6):506-514, June 2009. ilus, tab, graf.
Idioma: en.
Projeto: China National Natural Science Foundation; . Anhui Provincial Hospital. Science Research Foundation.
Resumo: Mesenchymal stem cells (MSCs) have been reported to secrete a variety of cytokines and growth factors acting as trophic suppliers, but little is known regarding the effects of conditioned medium (CM) of MSCs isolated from femurs and tibias of mouse on the artificial activation of mouse oocytes and on the developmental competence of the parthenotes. In the current study, we investigated the effect of CM on the events of mouse oocyte activation, namely oscillations of cytosolic calcium concentration ([Ca²+]i), meiosis resumption, pronucleus formation, and parthenogenetic development. The surface markers of MSCs were identified with a fluorescence-activated cell sorter. The dynamic changes of the spindle and formation of pronuclei were examined by laser-scanning confocal microscopy. Exposure of cumulus-oocyte complexes to CM for 40 min was optimal for inducing oocyte parthenogenetic activation and evoking [Ca²+]i oscillations similar to those evoked by sperm (95 vs 100 percent; P > 0.05). Parthenogenetically activated oocytes immediately treated with 7.5 µg/mL cytochalasin B (CB), which inhibited spindle rotation and second polar body extrusion, were mostly diploid (93 vs 6 percent, P < 0.01) while CB-untreated oocytes were mostly haploid (5 vs 83 percent, P < 0.01). Consequently, the blastocyst rate was higher in the CB-treated than in the CB-untreated oocytes. There was no significant difference in developmental rate between oocytes activated with CM and 7 percent ethanol (62 vs 62 percent, P > 0.05), but the developmental competence of the fertilized oocytes was superior to that of the parthenotes (88 vs 62 percent, P < 0.05). The present results demonstrate that CM can effectively activate mouse oocytes, as judged by the generation of [Ca²+]i oscillations, completion of meiosis and parthenogenetic development.
Descritores: Cálcio/metabolismo
Meios de Cultivo Condicionados/farmacologia
Citocalasina B/farmacologia
Células-Tronco Mesenquimais
Oócitos/efeitos dos fármacos
Partenogênese/efeitos dos fármacos
-Microscopia Confocal
Oócitos/fisiologia
Partenogênese/fisiologia
Limites: Animais
Masculino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  4 / 9 LILACS  
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Texto completo SciELO Brasil
Texto completo
Id: lil-428285
Autor: Yamamoto, D; Coimbra, V. C; Okuda, K; Rabinovitch, M.
Título: Enucleated L929 mouse fibroblasts support invasion and multiplication of Shigella flexneri 5a
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;39(6):749-758, June 2006. ilus.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico; . Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: Invasive bacteria can induce their own uptake and specify their intracellular localization; hence it is commonly assumed that proximate modulation of host cell transcription is not required for infection. However, bacteria can also modulate, directly or indirectly, the transcription of many host cell genes, whose role in the infection may be difficult to determine by global gene expression. Is the host cell nucleus proximately required for intracellular infection and, if so, for which pathogens and at what stages of infection? Enucleated cells were previously infected with Toxoplasma gondii, Chlamydia psittaci, C. trachomatis, or Rickettsia prowazekii. We enucleated L929 mouse fibroblasts by centrifugation in the presence of cytochalasin B, and compared the infection with Shigella flexneri M90T 5a of nucleated and enucleated cells. Percent infection and bacterial loads were estimated with a gentamicin suppression assay in cultures fixed and stained at different times after infection. Enucleation reduced by about half the percent of infected cells, a finding that may reflect the reduced endocytic ability of L929 cytoplasts. However, average numbers of bacteria and frequency distributions of bacterial numbers per cell at different times were similar in enucleated and nucleated cells. Bacteria with actin-rich tails were detected in both cytoplasts and nucleated cells. Lastly, cytoplasts were similarly infected 2 and 24 h after enucleation, suggesting that short-lived mRNAs were not involved in the infection. Productive S. flexneri infection could thus take place in cells unable to modulate gene transcription, RNA processing, or nucleus-dependent signaling cascades.
Descritores: Células L/microbiologia
Shigella flexneri/crescimento & desenvolvimento
-Citocalasina B
Núcleo Celular/microbiologia
Citoplasma/microbiologia
Fatores de Tempo
Limites: Animais
Camundongos
Responsável: BR1.1 - BIREME


  5 / 9 LILACS  
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Id: lil-339201
Autor: Vallejos A., Marcos.
Título: Deficiencia de transportadores de glucosa tipo I / Deficiency of glucose type I transporters
Fonte: Pediatría (Santiago de Chile);45:70-73, 2002. tab.
Idioma: es.
Descritores: Glucose
Proteínas de Transporte de Monossacarídeos/deficiência
-3-O-Metilglucose
Ácido Desidroascórbico/análise
Anticorpos Monoclonais
Citocalasina B
Doenças Metabólicas/diagnóstico
Limites: Humanos
Lactente
Tipo de Publ: Relatos de Casos
Responsável: CL1.1 - Biblioteca Central


  6 / 9 LILACS  
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Id: lil-319791
Autor: Cañizares, C; Vivar, N; Herdoiza, M.
Título: Role of the microtubular system in platelet aggregation
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;27(7):1533-1551, Jul. 1994.
Idioma: en.
Resumo: 1. Four structural systems are involved in the process of platelet activation that leads to aggregation: 1) the membrane system, i.e., the cytoplasmic membrane, the dense tubular structure and the open canalicular structure; 2) alpha and dense granules; 3) the peripheral microtubular coils; 4) the microfibrillar meshwork of actin-myosin bundles. 2. We added four compounds which modify cell ultrastructure to normal platelet-rich plasma to analyze the behavior of the structural systems of platelet activation: vinblastine (100 micrograms/ml) and cimetidine (100 micrograms/ml) that act on the membrane system, ticlopidine (200 micrograms/ml) and colchicine (100 micrograms/ml) that affect primarily the microtubular structure, cytochalasin B (30 micrograms/ml) and phorbol myristate acetate (100 ng/ml) that act upon the granular system, and cytochalasin D (30 micrograms/ml) and concanavalin A (50 micrograms/ml) that influence the microfibrillar structure. Platelet aggregation was stimulated by epinephrine or thrombin. 3. Cimetidine and ticlopidine prevented aggregation. However, neither substance affected the microtubular structure. Colchicine and cytochalasin B only partially impaired aggregation, because pieces of microtubules remained in the presence of these substances. The other substances did not present anti-aggregant activity and did not preserve the microtubules. 4. We infer that the disappearance of the microtubules is necessary to produce aggregation. When they remain intact no aggregation is produced, even though the other structural systems are activated.
Descritores: Agregação Plaquetária/fisiologia
Microtúbulos/fisiologia
-Agregação Plaquetária/efeitos dos fármacos
Plaquetas
Cimetidina
Colchicina
Concanavalina A
Citocalasina B
Citocalasina D
Acetato de Tetradecanoilforbol
Ticlopidina
Vimblastina
Limites: Humanos
Responsável: BR1.1 - BIREME


  7 / 9 LILACS  
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Texto completo SciELO Brasil
Mineo, J. R
Texto completo
Id: lil-281599
Autor: Reis, D. S; Souza, M. A; Mineo, J. R; Espindola, F. S.
Título: Myosin V and iNOS expression is enhanced in J774 murine macrophages treated with IFN-gamma
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;34(2):221-226, Feb. 2001.
Idioma: en.
Conferência: Apresentado em: Annual Meeting of the Federação de Sociedades de Biologia Experimental, 15, Caxambu, August 23-26, 2000.
Resumo: Actin-based motor protein requirements and nitric oxide (NO) production are important features of macrophage activity during phagocytosis or microbicidal processes. Different classes of myosins contribute directly or indirectly to phagocytosis by providing mechanical force for phagosome closure or organelle movement. Recent data have shown the presence of myosins IC, II, V and IXb in phagosomes of bone marrow-derived murine macrophages. In our investigation we demonstrated the presence of different classes of myosins in J774 macrophages. We also analyzed the effect of gamma interferon (IFN-gamma), with or without calcium ionophore or cytochalasin B, on myosins as well as on inducible nitric oxide synthase (iNOS) expression and NO production. Myosins IC, II, Va, VI and IXb were identified in J774 macrophages. There was an increase of myosin V expression in IFN-gamma-treated cells. iNOS expression was increased by IFN-gamma treatment, while calcium ionophore and cytochalasin B had a negative influence on both myosin and iNOS expression, which was decreased. The increases in NO synthesis were reflected by increased iNOS expression. Macrophages activated by IFN-gamma released significant amounts of NO when compared to control groups. In contrast, NO production by calcium ionophore- and cytochalasin B-treated cells was similar to that of control cells. These results suggest that IFN-gamma is involved in macrophage activation by stimulating protein production to permit both phagocytosis and microbicidal activity
Descritores: Células Cultivadas
Interferon gama/farmacologia
Macrófagos/efeitos dos fármacos
Proteínas do Tecido Nervoso/efeitos dos fármacos
Óxido Nítrico Sintase/efeitos dos fármacos
Óxido Nítrico/metabolismo
Fagocitose/efeitos dos fármacos
-Western Blotting
Estudos de Casos e Controles
Movimento Celular/efeitos dos fármacos
Citocalasina B
Ionóforos
Miosinas/efeitos dos fármacos
Limites: Animais
Camundongos
Responsável: BR1.1 - BIREME


  8 / 9 LILACS  
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Barbosa, H. S
Id: lil-3065
Autor: Ebert, F; Barbosa, H. S.
Título: The influence of cytochalasin B on the interaction of T cruzi and mouse peritoneal macrophages.
Fonte: Rev. Inst. Med. Trop. Säo Paulo;23(2):61-7, 1981.
Idioma: en.
Descritores: Citocalasina B
Macrófagos
Trypanosoma cruzi
Responsável: BR1.1 - BIREME


  9 / 9 LILACS  
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Visconti, Maria Aparecida
Castrucci, Ana Maria de Lauro
Id: lil-1287
Autor: Visconti, Maria Aparecida; Castrucci, Ana Maria de Lauro.
Título: Microtubule - and microfilament - disrupting drugs and melanosome migration in melanophores of Papiliochromis ramirezi (Cichlidae, Teleostei)
Fonte: An. acad. bras. ciênc;57(2):233-7, jun. 1985. tab.
Idioma: en.
Descritores: Citoesqueleto de Actina/efeitos dos fármacos
Colchicina/farmacologia
Citocalasina B/farmacologia
Melanócitos/fisiologia
Melanóforos/metabolismo
Microtúbulos/efeitos dos fármacos
Vimblastina/farmacologia
-Agregação Celular/efeitos dos fármacos
Peixes
Norepinefrina/farmacologia
Pigmentação/efeitos dos fármacos
Teofilina/farmacologia
Limites: Animais
Responsável: BR1.1 - BIREME



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