Base de dados : LILACS
Pesquisa : D03.633.100.759.138.525.400 [Categoria DeCS]
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Texto completo SciELO Chile
Texto completo
Id: biblio-1011433
Autor: Isah, Tasiu.
Título: De novo in vitro shoot morphogenesis from shoot tip-induced callus cultures of Gymnema sylvestre (Retz.) R. Br. ex Sm
Fonte: Biol. Res;52:3, 2019. tab, graf.
Idioma: en.
Resumo: BACKGROUND: Gymnema sylvestre is a medicinal woody perennial vine known for its sweetening properties and antidiabetic therapeutic uses in the modern and traditional medicines. Its over-exploitation for the therapeutic uses and to meet the demand of pharmaceutical industry in raw materials supply for the production of anti-diabetic drugs has led to considerable decline in its natural population. RESULTS: An efficient system of shoot bud sprouting from nodal segment explants and indirect plant regeneration from apical meristem-induced callus cultures of G. sylvestre have been developed on Murashige and Skoog (MS) medium amended with concentrations of cytokinins. Of the three growth regulators tested, N6-benzylaminopurine (BAP) was the most efficient and 2.0 mg L-1 gave the best shoot formation efficiency. This was followed by thidiazuron (TDZ) and kinetin (Kin) but, most of the TDZ-induced micro shoots showed stunted growth. Multiple shoot formation was observed on medium amended with BAP or TDZ at higher concentrations. The produced micro shoots were rooted on half strength MS medium amended with auxins and rooted plantlets acclimatized with 87% survival of the regenerates. CONCLUSIONS: The developed regeneration system can be exploited for genetic transformation studies, particularly when aimed at producing its high yielding cell lines for the anti-diabetic phytochemicals. It also offers opportunities for exploring the expression of totipotency in the anti-diabetic perennial vine.
Descritores: Reguladores de Crescimento de Plantas/farmacologia
Regeneração/efeitos dos fármacos
Brotos de Planta/crescimento & desenvolvimento
Gymnema sylvestre/crescimento & desenvolvimento
Morfogênese/efeitos dos fármacos
-Compostos de Fenilureia/farmacologia
Compostos de Benzil/farmacologia
Brotos de Planta/efeitos dos fármacos
Gymnema sylvestre/efeitos dos fármacos
Responsável: CL1.1 - Biblioteca Central

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Id: biblio-1016080
Autor: Oppermann, Tobias; Busse, Nadine; Czermak, Peter.
Título: Mannheimia haemolytica growth and leukotoxin production for vaccine manufacturing: a bioprocess review
Fonte: Electron. j. biotechnol;28:95-100, July. 2017. tab.
Idioma: en.
Resumo: Mannheimia haemolytica leukotoxin (LKT) is a known cause of bovine respiratory disease (BRD) which results in severe economic losses in the cattle industry (up to USD 1 billion per year in the USA). Vaccines based on LKT offer the most promising measure to contain BRD outbreaks and are already commercially available. However, insufficient LKT yields, predominantly reflecting a lack of knowledge about the LKT expression process, remain a significant engineering problem and further bioprocess optimization is required to increase process efficiency. Most previous investigations have focused on LKT activity and cell growth, but neither of these parameters defines reliable criteria for the improvement of LKT yields. In this article, we review the most important process conditions and operational parameters (temperature, pH, substrate concentration, dissolved oxygen level, medium composition and the presence of metabolites) from a bioprocess engineering perspective, in order to maximize LKT yields.
Descritores: Toxinas Bacterianas/biossíntese
Mannheimia haemolytica/metabolismo
Complexo Respiratório Bovino/microbiologia
Mannheimia haemolytica/patogenicidade
Concentração de Íons de Hidrogênio
Limites: Animais
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central

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Id: biblio-1015841
Autor: Maita, Segundo; Sotomayor, Carlos.
Título: Kernel size and weight affected by three plant bioregulators applied at bloom to Non Pareil and Carmel almond cultivars
Fonte: Electron. j. biotechnol;28:47-51, July. 2017. tab.
Idioma: en.
Resumo: Background: The yield of almonds [Prunus dulcis (Mill.) D.A. Webb] could be low due to climatic problems and any factor improving kernel size and weight, such as the use of plant bioregulators (PBRs), should be beneficial. Results: Three plant bioregulators: 24-epibrassinolide (BL), gibberellic acid (GA3) and kinetin (KN) were applied at three spray concentrations to Non Pareil and Carmel cultivars, at two phenological stages during bloom, in the 2014 and 2015 seasons. The results showed significant differences (P b 0.0001). For total dry weight of Non Pareil, the best treatment was BL (30 mg·L-1), with an average of 1.45 g, while the control was 1.30 g, at pink button during 2015. For Carmel, the best dry weight was 1.23 g, achieved with BL (30 mg·L-1) at fallen petals in both seasons. The average dry weight of the controls varied between 1.13 and 1.18 g. The greatest almond lengths and widths in Non Pareil were 24.98 mm and 15.05 mm, achieved with BL (30 mg·L-1) and KN (50 µL·L-1) treatments, respectively, applied at pink button in 2015. In Carmel, the greatest length and width were 24.38 and 13.44 mm, obtained with BL (30 mg·L-1) applied at the stages of pink button and fallen petals, respectively, in 2015. The control reached lengths between 22.33 and 23.38 mm, and widths between 11.99 and 12.93 mm. Conclusions: The use of the bioregulators showed significant favorable effects on dry weight, length and width of kernels at harvest, in both cultivars.
Descritores: Reguladores de Crescimento de Plantas/metabolismo
Prunus dulcis/crescimento & desenvolvimento
Responsável: CL1.1 - Biblioteca Central

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Texto completo SciELO Chile
Texto completo
Id: lil-591907
Autor: Belide, Srinivas; Sajjalaguddam, Ramgopal Rao; Paladugu, Anitha.
Título: Cytokinin preconditioning enhances multiple shoot regeneration in Pongamia pinnata (L) Pierre - a potential, non-edible tree seed oil source for biodiesel
Fonte: Electron. j. biotechnol;13(6):3-4, Nov. 2010. ilus, tab.
Idioma: en.
Projeto: World Bank. Sreenidhi Institute of Science and Technology and Technical Education Quality Improvement Program.
Resumo: An efficient, highly reproducible protocol for multiple shoot induction and plant regeneration of Pongamia pinnata has been successfully developed using cotyledonary node explants. This study also demonstrates that preconditioning of explant stimulates production of multiple shoots from cotyledonary nodes of P. pinnata. The highest direct shoot regeneration (90 percent) with an average of 18.4 +/- 3.1 shoots/explant were obtained when cotyledonary node explants were excised from seedlings germinated on Murashige and Skoog (MS) media supplemented with benzyladenine (BA) 1 mg l-1, and subsequently cultured on MS media with 1 mgl-1 thidiazuron (TDZ). Scanning electron microscope observations of cotyledonary node (CN) explants excised from pre-conditioned and normal seedlings, revealed larger buds with rapid development in BA-preconditioned CN explants. The addition of adenine sulphate significantly increased the average number of shoots per explant. The highest direct shoot regeneration (93 percent) with an average of 32.2 +/- 0.93 shoots/explant was obtained from BA-preconditioned CN when cultured on MS media supplemented with 1 mg l-1 TDZ and 200 mg l-1 adenine sulphate (ADS). Repeated shoot proliferation was observed from BA preconditioned CN explants up to 3 cycles with an average of 15 shoots/explant/cycle when cultured on MS media supplemented with 1 mg l-1 TDZ and 150 mg l-1 L-glutamine, thus producing 45 shoots/CN explant. Shoots were elongated on hormone free MS media and rooted on 1/2 MS media supplemented with 1 mg l-1 of IBA. Rooted shoots were successfully acclimatized and established in soil with 80 percent success. The highly regenerative system developed in this investigation for this important tree could be a useful tool for genetic transformation.
Descritores: Adenina/farmacologia
Brotos de Planta/fisiologia
Compostos de Fenilureia/farmacologia
-Adenina/análogos & derivados
Brotos de Planta
Microscopia Eletrônica de Varredura
Reguladores de Crescimento de Plantas/farmacologia
Responsável: CL1.1 - Biblioteca Central

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Id: lil-67510
Autor: Carvalho, A. C. Campos de.
Título: Potassium concentration influences the gating of K+ channels from sarcoplasmic reticulum
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;22(1):107-9, 1989. ilus.
Idioma: en.
Conferência: Apresentado em: Annual Meeting of the Federaçäo de Sociedades de Biologia Experimental, 3, Caxambú, June 29-July 3, 1988.
Resumo: Gating of K+ channels from the sarcoplasmic reticulum (SR) is influenced by the K+ concentration in the bathing solution. the probability of finding the channel open (Po) increases nonlinearly with the K+ concentration. The current two-state model which considers only voltage-dependent rate constants for the SR K+ channel should be reconsidered in the light of the present results
Descritores: Canais Iônicos/metabolismo
Membranas Artificiais
Retículo Sarcoplasmático/metabolismo
Responsável: BR26.1 - Biblioteca Central

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