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Pesquisa : D04.210.500.925 [Categoria DeCS]
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Id: lil-683998
Autor: Gao, Zhengquan; Meng, Chunxiao; Gao, Hongzheng; Zhang, Xiaowen; Xu, Dong; Su, Yuanfeng; Wang, Yuanyuan; Zhao, Yuren; Ye, Naihao.
Título: Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR)
Fonte: Biol. Res;46(2):201-206, 2013. ilus, tab.
Idioma: en.
Projeto: National Natural Science Foundation of China; . National Natural Science Foundation of Shandong Province; . State Key Laboratory of Agricultural Microbiology; . Shandong University of Technology.
Resumo: The fresh-water green unicellular alga Haematococcus pluvialis is known to accumulate astaxanthin under stress conditions. In the present study, transcriptional expression of eight genes involved in astaxanthin biosynthesis exposed to EBR (25 and 50 mg/L) was analyzed using qRT-PCR. The results demonstrated that both 25 and 50 mg/L EBR could increase astaxanthin productivity and the eight carotenogenic genes were up-regulated by EBR with different expression profiles. Moreover, EBR25 induction had a greater influence on the transcriptional expression of ipi-1, ipi-2, crtR-B, lyc and crtO (> 5- fold up-regulation) than on psy, pds, bkt; EBR50 treatment had a greater effect on the transcriptional expression of ipi-2, pds, lyc, crtR-B, bkt and crtO than on ipi-1 and psy. Furthermore, astaxanthin biosynthesis under EBR was up-regulated mainly by ipi1־ and psy at the post-transcriptional level, pds, lyc, crtR-B, bkt and crtO at the transcriptional level and ipi-2 at both levels.
Descritores: Brassinosteroides/farmacologia
Carotenoides/biossíntese
Clorófitas/genética
Reguladores de Crescimento de Plantas/farmacologia
RNA Mensageiro/metabolismo
Esteroides Heterocíclicos/farmacologia
-Análise de Variância
Carotenoides/genética
Clorófitas/citologia
Reação em Cadeia da Polimerase em Tempo Real
RNA Mensageiro/genética
Transcrição Genética
Xantofilas/biossíntese
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: lil-456750
Autor: Howell, W. M; Keller III, G. E; Kirkpatrick, J. D; Jenkins, R. L; Hunsinger, R. N; McLaughlin, E. W.
Título: Effects of the plant steroidal hormone, 24-epibrassinolide, on the mitotic index and growth of onion (Allium cepa) root tips
Fonte: Genet. mol. res. (Online);6(1):50-58, 2007. ilus, tab.
Idioma: en.
Resumo: The purpose of the present study was to determine the effects of the steroidal plant hormone, 24-epibrassinolide (BL), on the mitotic index and growth of onion (Allium cepa) root tips. The classical Allium test was used to gather and quantify data on the rate of root growth, the stages of mitosis, and the number of mitoses in control and BL-treated groups of onions. Low doses of BL (0.005 ppm) nearly doubled the mean root length and the number of mitoses over that of controls. Intermediate doses of BL (0.05 ppm) also produced mean root lengths and number of mitoses that were significantly greater than those of the controls. The highest dose of BL (0.5 ppm) produced mean root lengths and number of mitoses that were less than control values, but the differences were not statistically significant. Examination of longitudinally sectioned root tips produced relatively similar results. This study confirms the suppositions of previous authors who have claimed that exogenously applied BL can increase the number of mitoses in plants, but failed to show cytogenetic data. This is the first report detailing the effects of BL on chromosomes and the cell cycle.
Descritores: Colestanóis/farmacologia
Mitose/efeitos dos fármacos
Cebolas/crescimento & desenvolvimento
Reguladores de Crescimento de Plantas/farmacologia
Raízes de Plantas/crescimento & desenvolvimento
Esteroides Heterocíclicos/farmacologia
-Índice Mitótico
Cebolas/efeitos dos fármacos
Raízes de Plantas/efeitos dos fármacos
Responsável: BR1.1 - BIREME



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