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Id: lil-541117
Autor: Capani, F; Saraceno, E; Boti, V. R; Aon-Bertolino, L; Fernández, J. C; Gato, F; Krause, M. S; Giraldez, L; Ellisman, M. H; Coirini, H.
Título: A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation
Fonte: Biocell;32(1):1-8, Apr. 2008. ilus.
Idioma: en.
Resumo: Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and three-dimensional reconstructions provide a valuable tool for revealing aspects of the synaptic morphology that are not available when conventional electron microscopy is used. By applying this selective staining technique and three-dimensional imaging, we uncovered the structural organization of actin in the postsynaptic densities in physiological and pathological conditions.
Descritores: Actinas/metabolismo
Amarelo de Eosina-(YS)/farmacologia
Amarelo de Eosina-(YS)/metabolismo
Foto-Oxidação
Sistema Nervoso Central/metabolismo
Sistema Nervoso Central/ultraestrutura
-Coloração e Rotulagem/métodos
Corantes Fluorescentes/farmacologia
Faloidina/farmacologia
Imageamento Tridimensional/métodos
Modelos Moleculares
Citoesqueleto de Actina/metabolismo
Citoesqueleto de Actina/ultraestrutura
Microscopia de Fluorescência/métodos
Oxirredução
Fótons
Limites: Humanos
Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo



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