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Id: biblio-844896
Autor: Díaz Domínguez, Gabriela; Marsán Suárez, Vianed; del Valle Pérez, Lázaro O.
Título: Principales propiedades inmunomoduladoras y antinflamatorias de la ficobiliproteína C-ficocianina / Main immunomodulatory and anti-inflamatory properties of phycobiliproteins C-phycocyanin
Fonte: Rev. cuba. hematol. inmunol. hemoter;32(4):447-454, oct.-dic. 2016.
Idioma: es.
Resumo: Las ficobiliproteínas son proteínas solubles en agua, que funcionan como pigmentos fotosintéticos accesorios en diferentes organismos tales como las cianobacterias, las algas rojas y las criptomonadas. En el alga verdeazul Spirulina platensis, una de las ficobiliproteínas más abundantes es la C-ficocianina, la cual tiene unido tres cromóforos ficocianobilina mediante un enlace tioéter a cisteínas específicas. La ficocianobilina es un tetrapirrol lineal asociado a la captación de energía solar en estos organismos. La C-ficocianina ha sido empleada en diferentes investigaciones biomédicas como biomarcador, por sus propiedades fluorescentes, y como posible agente terapéutico para el tratamiento de enfermedades asociadas al estrés oxidativo, por sus propiedades antioxidantes, inmunomoduladoras y antinflamatorias. Se ha demostrado que esta proteína aumenta la liberación de interferón gamma en células mononucleares de sangre periférica y modula la producción de citocinas inflamatorias como el factor de necrosis tumoral alfa, entre otras. Además, se ha encontrado que la C-ficocianina tiene efecto inmunomodulador de citocinas que potencian la activación de las células del sistema inmune, como la IL-6 y la IL-1ß, así como la regulación de aproximadamente 190 genes implicados en la inmunidad(AU)

Phycobiliproteins are water-soluble proteins that function as accessory photosynthetic pigments in different organisms such as cyanobacteria, red algae and cryptomonads. In the blue-green algae Spirulina platensis one of the most abundant phycobiliproteins is the C-phycocyanin, which has three phycocyanobilin chromophores linked through a thioether bond to specific cysteine. The phycocyanobilin is a linear tetrapyrrole associated with solar energy absorption in these organisms. The C-phycocyanin has been used in several biomedical researches as a biomarker, for their fluorescence properties, and as a possible therapeutic agent for the treatment of diseases associated with oxidative stress for its antioxidant, anti-inflammatory and immunomodulatory properties. It has been shown that this protein increases the release of interferon gamma in peripheral blood mononuclear cells, and modulates the production of inflammatory cytokines such as tumor necrosis factor among others. Furthermore it has been found that the C-phycocyanin has immunomodulatory effect on cytokines that enhance the activation of immune cells, such as IL-6 and IL-1ß, and the regulation of about 190 genes involved in immunity(AU)
Descritores: Ficobiliproteínas/uso terapêutico
-Fatores Imunológicos/uso terapêutico
Ficocianina/uso terapêutico
Tipo de Publ: Revisão
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: lil-703335
Autor: Jonte, Lorena; Rosales-Loaiza, Néstor; Bermúdez-González, José; Morales, Ever.
Título: Cultivos discontinuos alimentados con urea de la cianobacteria Phormidium sp. en función de la salinidad y edad del cultivo / Urea fed-batch cultures of the cyanobacterium Phormidium sp. as a function of the salinity and age of cultures
Fonte: Rev. colomb. biotecnol;15(2):38-46, jul.-dic. 2013. graf.
Idioma: es.
Resumo: Se comparó la eficiencia de sistemas de cultivos discontinuos alimentados versus cultivos discontinuos convencionales, en cuanto a concentración de nitrógeno, adicionando 0,2 mM de urea cada tres días al final de la fase exponencial, durante 21 días. Se realizaron cultivos con un volumen de 1500 mL a 15 y 35 UPS de salinidad, enriquecidos con medio ALGAL 8mM NaNO3, a 238 µmol q m-2 s-1, aireación constante, fotoperiodo 12:12 horas y temperatura de 29 ±3°C. Phormidium sp. posee la capacidad de hidrolizar la urea; mostrando una asimilación de 65±7,07% de la misma, con la mayor producción (p<0,05) de clorofila a, ficocianina y proteínas de 20,26±1,24; 203,47±12,83 y 707,87±28,47 µg mL-1en los cultivos alimentados. La producción de pigmentos vario en el tiempo, independientemente a la salinidad y sistema de cultivo, mientras que la producción de proteínas y carbohidratos totales fue directamente proporcional a la edad del cultivo, con valores máximos de 612,74 ± 5,41 µg mL-1 y 8,96±0,08 mg mL-1 respectivamente a los 31 días. La síntesis de lípidos y EPS fueron influenciadas (p<0,05) por la salinidad, presentando los máximos de lípidos a 15 UPS con 12,22±2,91µg mL-1, y los EPS se incrementaron a 35 UPS con 2,00 ± 0,26 y 2,03 ± 0,15 mg mL-1. Estos resultados determinan que los cultivos de Phormidium sp. alimentados con urea y a salinidades de 15 y 35 UPS, representan una alternativa económica para la producción de clorofila a, ficocianina y proteínas, incrementándose un 31,04; 40,72 y 31,94 % respectivamente en comparación con cultivos no alimentados.

Fed-batch system efficiency versus batch cultures was compared in relation to nitrogen concentration, adding 0,2mM urea at the end of the exponential phase, during 21 days. Cultures were carried out in 1500 mL to 1.5 and 3.5 UPS of salinity, enriched with Algal medium 8mM NaNO3, 238 mol q m-2 s-1, constant aeration, photoperiod 12:12 h. and 29 ±3°C. Phormidium sp. is able to hydrolyze urea; showing a total assimilation of 65±7.07%, with the highest (p< 0.05) chlorophyll a, phycocyanin and protein production of 20.26 ± 1.24, 203.47 ± 12.83 and 707.87 ± 28.47 µg mL-1 in the fed-batch cultures. On the other hand, pigment production varies in time, regardless salinity and culture system. Proteins and total carbohydrate production were directly proportional to the age of cultures, with maximum values of 612.74 ± 5.41 µg mL-1 and 8.96 ± 0.08 mg mL-1, respectively. Lipid and EPS were influenced (p< 0.05) by salinity, showing maximum of lipids at 15 UPS with 12.22±2.91 µg mL-1, and EPS at 15 and 35 UPS with 2.00 ± 0.26 and 2.03 ± 0.15 mg mL-1. These results determine that Phormidium sp. cultures fed with urea, to salinities of 15-35 UPS, represent an economic alternative for chlorophyll a, phycocyanin and protein production, with an increase of 31.04, 40.72 and 31.94% respectively in comparison with non-fed cultures.
Descritores: Cianobactérias/classificação
Cianobactérias/crescimento & desenvolvimento
Cianobactérias/química
Salinidade
Ureia/administração & dosagem
Ureia/isolamento & purificação
Ureia/análogos & derivados
Ureia/imunologia
Ureia/síntese química
Ureia
-Clorofila
Ficocianina
Proteínas
Responsável: CO326 - Departamento de Biología


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Id: lil-662141
Autor: Vega, Israel A.; Dellagnola, Federico A.; Hurst, Jorge A.; Godoy, Martín S.; Castro-Vazquez, Alfredo.
Título: A study of chlorophyll-like and phycobilin pigments in the C endosymbiont of the apple- snail Pomacea canaliculata
Fonte: Biocell;36(2):47-55, Aug. 2012. graf.
Idioma: en.
Resumo: Pigments present in the brown-greenish C morph of an intracellular endosymbiont of Pomacea canaliculata were investigated. Acetone extracts of the endosymbiotic corpuscles showed an absorption spectrum similar to that of chlorophylls. Three fractions obtained from silica gel column chromatography of the acetone extracts (C I, C II and C III), were studied by positive ion fast atom bombardment-mass spectrometry (FAB-MS) and hydrogen-nuclear magnetic resonance (H-NMR). Results indicated the presence of (1) a sterol in the yellow colored C I fraction; (2) a mixture of pheophorbides a and b in the major green fraction, C II; and (3) a modified pheophorbide a in the smaller green fraction, C III. Aqueous extracts of the C endosymbiont did not show evidence of the occurrence of C-phycocyanin, allophycocyanin or phycoerithrin (light absorption, fluorescence emission, and electrophoresis of the protein moieties) while cyanobacterial cells (Nostoc sp.) showed evidence of C-phycocyanin and allophycocyanin. The possible phylogenetic and functional significance of the pigments present in the C endosymbiont is discussed.
Descritores: Clorofila/análise
Malus/crescimento & desenvolvimento
Ficobilinas/análise
Simbiose
Caramujos/crescimento & desenvolvimento
-Espectroscopia de Ressonância Magnética
Malus/metabolismo
Ficocianina/metabolismo
Espectrometria de Massas de Bombardeamento Rápido de Átomos
Caramujos/metabolismo
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Id: lil-622823
Autor: Deshmukh, Devendra V; Puranik, Pravin R.
Título: Statistical evaluation of nutritional components impacting phycocyanin production in Synechocystis SP
Fonte: Braz. j. microbiol;43(1):348-355, Jan.-Mar. 2012. ilus, tab.
Idioma: en.
Resumo: Alkaliphilic cyanobacterial cultures were isolated from Lonar lake (MS, India). Among the set of cultures, Synechocystis sp, was studied for phycocyanin production. A maximum yield was obtained in BG-11 medium at optimized conditions (pH 10 and 16 h light). In order to increase the phycocyanin yield media optimization based on the eight media components a Plackett-Burman design of the 12 experimental trials was used. As per the analysis CaCl2.2H2O and Na2CO3 have been found to be the most influencing media components at 95% significance. Further the optimum concentrations of these components were estimated following a Box Wilson Central Composite Design (CCD) with four star points and five replicates at the center points for each of two factors was adopted for optimization of these two media components. The results indicated that there was an interlinked influence of CaCl2.2H2O and Na2CO3 on 98% significance. The maximum yield of phycocyanin (12% of dry wt) could be obtained at 0.058 g/l and 0.115 g/l of CaCl2.2H2O and Na2CO3, respectively.
Descritores: Alcalinidade da Água/métodos
Cianobactérias/isolamento & purificação
Ficocianina
Phycomyces/isolamento & purificação
Carbonato de Sódio
Análise Estatística
Synechocystis/isolamento & purificação
-Fenômenos Fisiológicos Bacterianos
Fluorescência
Laguna Costeira
Métodos
Técnicas
Amostras de Água
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-452561
Autor: Colla, Luciane Maria; Furlong, Eliana Badiale; Costa, Jorge Alberto Vieira.
Título: Antioxidant properties of Spirulina (Arthospira) platensis cultivated under different temperatures and nitrogen regimes
Fonte: Braz. arch. biol. technol;50(1):161-167, Jan. 2007. tab, graf.
Idioma: en.
Resumo: The main aim of this work was to investigate the effects of temperature and nitrogen concentration on the antioxidant potential of extracts from Spirulina (Arthospira) platensis biomass. S. platensis biomass obtained at 35°C and with 1.875 g.L-1 or 2.5 g.L-1 of sodium nitrate in the culture medium presented higher concentrations of phenolic compounds. The antioxidant potential of methanol extracts of biomass on the enzymatic browning caused by peroxidase were 29 and 35 percent, respectively, being the reduction related to the amount of phenolic compounds present in this extract.

A cianobactéria Spirulina tem sido comercializada e estudada devido as suas propriedades nutricionais e terapêuticas no tratamento de doenças como o câncer, a hipercolesterolemia e a aterosclerose. Determinados compostos presentes na Spirulina, como os compostos fenólicos, a ficocianina e o tocoferol, podem apresentar atividade antioxidante. O principal objetivo deste trabalho foi avaliar o efeito da temperatura de cultivo e da concentração de nitrogênio no meio de cultivo, sobre o potencial antioxidante da microalga Spirulina (Arthospira) platensis. Quando a microalga foi cultivada a 35°C e concentrações de nitrato de sódio de 1,875 g.L-1 ou 2,5 g.L-1, o potencial antioxidante dos extratos obtidos a partir da biomassa, sobre o escurecimento enzimático causado pela peroxidase, foi de 29 por cento e 35 por cento, respectivamente, sendo a redução no escurecimento relacionada com as quantidades de compostos fenólicos presentes nos extratos.
Descritores: Antioxidantes
Arteriosclerose/terapia
Clorófitas
Cianobactérias
Aditivos Alimentares
Hipercolesterolemia/terapia
Neoplasias/terapia
Fenol
Ficocianina
Responsável: BR16.1 - Biblioteca de Ciências da Saúde


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Id: lil-448783
Autor: Yongzhong, Lu; Zhang, Xuecheng.
Título: The upstream sequence of the phycocyanin b subunit gene from Arthrospira platensis regulates expression of gfp gene in response to light intensity
Fonte: Electron. j. biotechnol;8(1):63-70, Apr. 2005. ilus, tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: In cyanobacteria, few details are known of the mechanisms through which the expression of the light-harvesting pigment c-phycocyanin is regulated. In the present study, a 419 bp upstream sequence of the phycocyanin b subunit (cpcB) gene from Arthrospira platensis FACHB341 was fused with green fluorescent protein (gfp) gene, and a heterologous reporting system was built up to investigate the influence of light intensity on the expression of gfp gene, and the regulation function of different region of the upstream sequence of cpcB gene. Results showed that the upstream sequence of cpcB gene could drive the expression of gfp gene in Synechococcus sp. strain PCC7942, and the expression was influenced by light intensity, the lower the light intensity, the higher the GFP level. Deletion analysis revealed that a light-responsive element was located in the region -276 to-218, a promoter sequence was in the region -85 to -1, and two positive cis elements were in the -419 to -276 and the -218 to -130 regions, respectively.
Descritores: Cianobactérias/genética
Ficocianina/metabolismo
Proteínas Luminescentes/genética
Proteínas Luminescentes/metabolismo
-Clonagem Molecular
Vetores Genéticos
Proteínas de Fluorescência Verde
Luz
Mutagênese
Regiões Promotoras Genéticas
Recombinação Genética
Deleção de Sequência
Synechococcus/genética
Transformação Genética
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Venezuela
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Id: lil-338637
Autor: Morales, Ever; Rodríguez, Marisa; García, Digna; Loreto, César; Marco, Eduardo.
Título: Crecimiento, producción de pigmentos y exopolisacáridos de la cianobacteria anabaena sp. PCC 7120 en función del pH y CO2 / Effect of pH and CO2 on growth, pigments and exopolysaccharides production from cianobacteria anaebaena sp. PCC 7120
Fonte: Interciencia;27(7):373-378, jul. 2002. tab, graf.
Idioma: es.
Resumo: El crecimiento producción de clorofila a, ficocianina, carotenoides y exopolisacáridos (EPS) de la cianobacteria filamentosa Anabaena sp. PCC 7120 fueron analizados en función del pH (5,5-10,5) con o sin soluciones amortiguadoras en cultivos discontinuos y en función del CO2 (0,03 y 5,0 por ciento) en cultivos semicontinuos a una tasa de renovación del 10 por ciento. El crecimiento de la cianobacteria se analizó por turbidez a 750nm y recuento celular, y la actividad fotosintética mediante uso de un electrodo de O2. Los cultivos, por triplicado, se mantuvieron con aireación constante, a 28 ñ 2ºC y con iluminación continua o fotoperíodo según el experimento. El pH y tampón utilizado influyeron en el crecimiento. El mayor crecimiento y contenido de EPS se alcanzaron, respectivamente, a pH 8,0-9,0 y 10,0. Sin embargo, los cultivos no tamponados resultaron con mayor contenido de clorofila a y ficocianina. El crecimiento, la actividad fotosintética y los carotenoides no variaron con la adición de CO2. Los cultivos semicontinuos con bajo CO2 produjeron los valores más elevados de clorofila a, ficocianina y exopolisacáridos, con 25,9 ñ 1,69, 2010 ñ 22,61 y 2286,0 ñ 42,76 µg.ml-1, respectivamente. El contenido de ficocianina y de EPS fue de 2,7 y 4 veces superior al obtenido a altos niveles de CO2. El pH y el sistema semicontinuo constituyen herramientas importantes para modular el crecimiento y contenido de pigmentos y de EPS de Anabaena sp. PCC 7120
Descritores: Anabaena
Carotenoides
Clorofila
Concentração de Íons de Hidrogênio
Ficocianina
Pigmentos Biológicos
-Ciência
Venezuela
Tipo de Publ: Relatos de Casos
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Id: lil-309283
Autor: Romay, Cheyla; Remírez, Diadelis; González, Ricardo.
Título: Actividad antioxidante de la ficocianina frente a radicales peroxílicos y la peroxidación lipídica microsomal / Antioxidant activity of phycocyaning againts peroxyl radicals and microsomal lipid peroxidation
Fonte: Rev. cuba. invest. bioméd;20(1):38-41, ene.-mar. 2001. tab, graf.
Idioma: es.
Resumo: La ficocianina es una biliproteína que se extrae de las algas verdeazules como la Spirulina (Arthospira) máxima. Esta proteína porta 3 grupos cromogénicos (tetrapirroles lineales) por unidad monométrica, que poseen una estructura muy similar a la de la bilirrubina, que es un reconocido antioxidante endógeno. Por otra parte se conoce que otros derivados de las porfirinas, como la clorofila alfa, presentan propiedades antioxidantes. Teniendo en cuenta estos antecedentes, en este trabajo el propósito fue evaluar si la ficocianina presentaba propiedades antioxidantes frente a radicales peroxílicos en diferentes condiciones experimentales como fueron: 1. disminución de la banda de absorción a 620 nm, asociada con la integridad del cromógeno, al reaccionar con radicales peroxílicos; 2. protección de hematíes humanos de la lisis inducida por radicales peroxílicos; 3. inhibición de la peroxidación lipídica microsomal inducida por Fe+2 -ascórbico. Los resultados indicaron que los grupos cromóforos de la ficocianina reaccionaban rápidamente con los radicales peroxílicos, mecanismo por el cual la biliproteína era capaz de proteger los hematíes de la lisis celular y de inhibir la peroxidación lipídica microsomal
Descritores: Antioxidantes
Peroxidação de Lipídeos
Ficocianina
Extratos Vegetais
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: lil-241598
Autor: Pádula, M.; Boiteux, S..
Título: Photodynamic DNA damage induced by phycocyanin and its repair in Saccharomyces cerevisiae
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;32(9):1063-71, Sept. 1999.
Idioma: en.
Resumo: In the present study, we analyzed DNA damage induced by phycocyanin (PHY) in the presence of visible light (VL) using a set of repair endonucleases purified from Escherichia coli. We demonstrated that the profile of DNA damage induced by PHY is clearly different from that induced by molecules that exert deleterious effects on DNA involving solely singlet oxygen as reactive species. Most of PHY-induced lesions are single strand breaks and, to a lesser extent, base oxidized sites, which are recognized by Nth, Nfo and Fpg enzymes. High pressure liquid chromatography coupled to electrochemical detection revealed that PHY photosensitization did not induce 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) at detectable levels. DNA repair after PHY photosensitization was also investigated. Plasmid DNA damaged by PHY photosensitization was used to transform a series of Saccharomyces cerevisiae DNA repair mutants. The results revealed that plasmid survival was greatly reduced in rad14 mutants, while the ogg1 mutation did not modify the plasmid survival when compared to that in the wild type. Furthermore, plasmid survival in the ogg1 rad14 double mutant was not different from that in the rad14 single mutant. The results reported here indicate that lethal lesions induced by PHY plus VL are repaired differently by prokaryotic and eukaryotic cells. Morever, nucleotide excision repair seems to play a major role in the recognition and repair of these lesions in Saccharomyces cerevisiae
Descritores: Dano ao DNA
Reparo do DNA
DNA/efeitos da radiação
Luz
Fármacos Fotossensibilizantes/farmacologia
Ficocianina/farmacologia
Saccharomyces cerevisiae/efeitos dos fármacos
-Meios de Cultura
N-Glicosil Hidrolases/fisiologia
Ficocianina/uso terapêutico
Lesões por Radiação
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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