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Id: biblio-950087
Autor: Khanizadeh, Fatemeh; Rahmani, Asghar; Asadollahi, Khairollah; Ahmadi, Mohammad Reza Hafezi.
Título: Combination therapy of curcumin and alendronate modulates bone turnover markers and enhances bone mineral density in postmenopausal women with osteoporosis
Fonte: Arch. endocrinol. metab. (Online);62(4):438-445, July-Aug. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Objective: This study evaluated the effects of combination therapy of curcumin and alendronate on BMD and bone turnover markers in postmenopausal women with osteoporosis. Subjects and methods: In a randomized, double-blind trial study, 60 postmenopausal women were divided into three groups: control, alendronate, and alendronate + curcumin. Each group included 20 patients. Total body, total hip, lumbar spine and femoral neck BMDs were measured by dual-energy X-ray absorptiometry (DXA) at baseline and after 12 months of therapy. Bone turnover markers such as bone-specific alkaline phosphatase (BALP), osteocalcin and C-terminal cross-linking telopeptide of type I collagen (CTx) were measured at the outset and 6 months later. Results: Patients in the control group suffered a significant decrease in BMD and increased bone turnover markers at the end of study. The group treated with only alendronate showed significantly decreased levels of BALP and CTx and increased levels of osteocalcin compared to the control group. The alendronate group also showed significant increases in the total body, total hip, lumbar spine and femoral neck BMDs at the end of study compared to the control group. In the curcumin + alendronate group, BALP and CTx levels decreased and osteocalcin levels increased significantly at the end of study compared to the control and alendronate groups. BMD indexes also increased in four areas significantly at the end of study compared to the control and alendronate groups. Conclusion: The combination of curcumin and alendronate has beneficial effects on BMD and bone turnover markers among postmenopausal women with osteoporosis. Arch Endocrinol Metab. 2018;62(4):438-45
Descritores: Densidade Óssea/efeitos dos fármacos
Osteoporose Pós-Menopausa/metabolismo
Alendronato/farmacologia
Curcumina/farmacologia
Conservadores da Densidade Óssea/farmacologia
-Fragmentos de Peptídeos/efeitos dos fármacos
Fragmentos de Peptídeos/urina
Osteocalcina/análise
Osteocalcina/efeitos dos fármacos
Método Duplo-Cego
Remodelação Óssea/efeitos dos fármacos
Colágeno Tipo II/efeitos dos fármacos
Colágeno Tipo II/urina
Quimioterapia Combinada/métodos
Fosfatase Alcalina/análise
Fosfatase Alcalina/efeitos dos fármacos
Limites: Humanos
Feminino
Pessoa de Meia-Idade
Idoso
Tipo de Publ: Ensaio Clínico Controlado Aleatório
Responsável: BR1.1 - BIREME


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Id: lil-767059
Autor: Egydio, Fernanda M.; Freitas Filho, Luiz G.; Sayeg, Kleber; Laks, Marcus; Oliveira, Andréia S.; Almeida, Fernando G..
Título: Acellular human glans extracellular matrix as a scaffold for tissue engineering: in vitro cell support and biocompatibility
Fonte: Int. braz. j. urol;41(5):990-1001, Sept.-Oct. 2015. graf.
Idioma: en.
Resumo: ABSTRACT Objectives: Diseases of the genitourinary tract can lead to significant damage. Current reconstructive techniques are limited by tissue availability and compatibility. This study aims to assess if the decellularized human glans can be used as a biomaterial for penile reconstruction. Materials and Methods: Samples of the glans matrices were descellularized. We evaluate the presence of collagen type I and III, and elastic fibers. Biocompatibility assays were performed to assess the cytotoxic and non-cytotoxic interactions between the acellular matrix and 3T3 cells. The matrices were seeded with mesenchymal stem cells and were assessed for viability and integration of these cells. Biomechanical tests in native tissue, descellularized matrix and seeded matrix were performed to characterize their biomechanical properties. Results: The tissue architecture of the decellularized matrix of human glans was preserved as well as the maintenance of the biomechanical and biological properties. The analyzes of glans seeded with mesenchymal stem cells revealed the integration of these cells to the matrices, and its viability during two weeks "in vitro". Conclusion: The decellularization process did not alter the biological and biomechanical characteristics of the human glans. When these matrices were seeded they were able to maintain the cells integrity and vitality.
Descritores: Materiais Biocompatíveis
Matriz Extracelular/fisiologia
Células-Tronco Mesenquimais/fisiologia
Pênis/citologia
Tecidos Suporte
Engenharia Tecidual/métodos
-/fisiologia
ABATTOIRSTABATTOIRS CELLS/fisiologia
Fenômenos Biomecânicos
Células Cultivadas
Colágeno Tipo I/análise
Colágeno Tipo II/análise
Teste de Materiais
Células-Tronco Mesenquimais/citologia
Ratos Wistar
Reprodutibilidade dos Testes
Fatores de Tempo
Limites: Animais
Humanos
Masculino
Camundongos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: lil-762916
Autor: Simental-Mendía, M.; Lara-Arias, J.; Álvarez-Lozano, E.; Said-Fernández, S.; Soto-Domínguez, A.; Padilla-Rivas, G. R.; Martínez-Rodríguez, H. G..
Título: Cotransfected human chondrocytes: over-expression of IGF-I and SOX9 enhances the synthesis of cartilage matrix components collagen-II and glycosaminoglycans
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;48(12):1063-1070, Dec. 2015. tab, graf.
Idioma: en.
Resumo: Damage to cartilage causes a loss of type II collagen (Col-II) and glycosaminoglycans (GAG). To restore the original cartilage architecture, cell factors that stimulate Col-II and GAG production are needed. Insulin-like growth factor I (IGF-I) and transcription factor SOX9are essential for the synthesis of cartilage matrix, chondrocyte proliferation, and phenotype maintenance. We evaluated the combined effect of IGF-I and SOX9 transgene expression on Col-II and GAG production by cultured human articular chondrocytes. Transient transfection and cotransfection were performed using two mammalian expression plasmids (pCMV-SPORT6), one for each transgene. At day 9 post-transfection, the chondrocytes that were over-expressing IGF-I/SOX9 showed 2-fold increased mRNA expression of the Col-II gene, as well as a 57% increase in Col-II protein, whereas type I collagen expression (Col-I) was decreased by 59.3% compared with controls. The production of GAG by these cells increased significantly compared with the controls at day 9 (3.3- vs 1.8-times, an increase of almost 83%). Thus, IGF-I/SOX9 cotransfected chondrocytes may be useful for cell-based articular cartilage therapies.
Descritores: Condrócitos/metabolismo
Colágeno Tipo II/biossíntese
Glicosaminoglicanos/biossíntese
Fator de Crescimento Insulin-Like I/metabolismo
Proteínas Matrilinas/biossíntese
Fatores de Transcrição SOX9/metabolismo
Transfecção/métodos
-Cartilagem Articular/lesões
Cartilagem Articular/metabolismo
Colágeno Tipo II/análise
Matriz Extracelular/química
Expressão Gênica
Glicosaminoglicanos/análise
Fator de Crescimento Insulin-Like I/genética
Proteínas Matrilinas/genética
Cultura Primária de Células
Reação em Cadeia da Polimerase em Tempo Real
RNA Mensageiro/metabolismo
Fatores de Transcrição SOX9/genética
Espectrofotometria
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-736433
Autor: Silva, Paulo Sérgio Cardoso da; Boing, Antonio Fernando; Peres, Karen Glazer.
Título: Redução das desigualdades no uso de consultas médicas no Brasil: análise das regiões Nordeste e Sudeste entre 2003 e 2008 / Reduction of inequalities in medical visits in Brazil: analysis of the Northeastern and Southeastern regions between 2003 and 2008
Fonte: Rev. bras. epidemiol;18(1):248-261, Jan-Mar/2015. tab, graf.
Idioma: pt.
Resumo: OBJETIVO: Analisar as desigualdades socioeconômicas na utilização de consultas médicas (CM) no último ano no Brasil. MÉTODOS: Dados da Pesquisa Nacional por Amostra de Domicílios (≥ 20 anos de idade) das Regiões Nordeste (2003, n = 75.652 e 2008, n = 79.779) e Sudeste (2003, n = 76.029 e 2008, n = 79.356) foram analisados segundo CM. Compararam-se as prevalências de CM segundo as variáveis exploratórias demográficas e de saúde no primeiro (D1) e último (D10) decil de renda familiar per capita. As análises consideraram o desenho amostral complexo. RESULTADOS: A proporção de pessoas com CM aumentou no período na Região Nordeste (61,2 para 66,9%) e Sudeste (67,9 para 73,5%). A diferença absoluta de CM, segundo D1 e D10 no período, foi de 6,4 pontos percentuais (pp) no Nordeste e 4,2 pp no Sudeste. Houve importante redução das desigualdades entre os homens; naqueles sem doenças crônicas; naqueles que tinham uma percepção positiva da sua saúde e naqueles sem plano de saúde com direito a CM. A Região Sudeste ainda apresentou redução entre aqueles com apenas uma morbidade autorreferida (8 pp) e com percepção negativa da saúde (6 pp). CONCLUSÃO: Houve aumento de CM no Brasil. Observa-se ainda persistente desigualdade entre os mais pobres e os mais ricos, maior no Nordeste do que no Sudeste. Políticas para a redução da desigualdade em saúde mais eficazes e equânimes devem ser adotadas no Brasil. .

OBJECTIVE: To analyze the socioeconomic inequalities in medical visits (MV) in the past year in Brazil. METHODS: Data from adults aged ≥ 20 years old who participated in the Brazilian National Household Surveys and living in the Northeastern (2003; n = 75,652 and 2008, n = 79,779) and Southeastern (2003; n = 76,029 and 2008; n = 79,356) regions were analyzed according to MV. We compared MVs according to demographic and health variables in the first (D1) and last (D10) per capita family income deciles. All analyses considered the complex cluster design. RESULTS: The proportion of people who had MV during this period increased in the Northeastern (from 61.2 to 66.9%) and the Southeastern (from 67.9 to 73.5%) regions. The absolute difference (AD) in the use of MV, according to D1 and D10 in this period, was equal to 6.4 percentage points (pp) in the Northeastern and 4.2 pp in the Southeastern regions. Significant reduction in inequalities was observed among men without chronic diseases, in those who had a positive perception of their health, and among those without health insurance which included MV. The Southeastern region has also showed significant reduction among those with chronic disease (8 pp) and with negative health self-perception (6 pp). CONCLUSION: The increasing number of MVs was found in Brazil. However, persistent inequalities were observed between the poorest and the richest, higher in the Northeastern than in the Southeastern region. More effective and equitable policies to reduce health inequalities should be adopted in Brazil. .
Descritores: Adenocarcinoma/patologia
Neoplasias do Colo/patologia
Neoplasias Hepáticas/patologia
Neoplasias Hepáticas/secundário
Metástase Neoplásica/patologia
-Adenocarcinoma/tratamento farmacológico
Antimetabólitos Antineoplásicos/uso terapêutico
Antineoplásicos Fitogênicos/uso terapêutico
Adesão Celular
Linhagem Celular Tumoral
Camptotecina/análogos & derivados
Camptotecina/uso terapêutico
Colágeno Tipo II/metabolismo
Neoplasias do Colo/tratamento farmacológico
Fibronectinas/metabolismo
Floxuridina/uso terapêutico
Fluoruracila/uso terapêutico
Laminina/metabolismo
Neoplasias Hepáticas/tratamento farmacológico
Camundongos Nus
Modelos Biológicos
MICE, INBRED CABDOMENABDOMINAL INJURIESBL
Metástase Neoplásica/prevenção & controle
Paclitaxel/uso terapêutico
Limites: Animais
Feminino
Humanos
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-716279
Autor: Xu, G.J.; Lu, Z.H.; Lin, X.; Lin, C.W.; Zheng, L.; Zhao, J.M..
Título: Effect of JJYMD-C, a novel synthetic derivative of gallic acid, on proliferation and phenotype maintenance in rabbit articular chondrocytes in vitro
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(8):637-645, 08/2014. tab, graf.
Idioma: en.
Projeto: the National Natural Science Foundation of China; . the National Natural Science Foundation of China; . the Guangxi Natural Science Foundation Program of China; . the Open Project of Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards.
Resumo: Tissue engineering encapsulated cells such as chondrocytes in the carrier matrix have been widely used to repair cartilage defects. However, chondrocyte phenotype is easily lost when chondrocytes are expanded in vitro by a process defined as “dedifferentiation”. To ensure successful therapy, an effective pro-chondrogenic agent is necessary to overcome the obstacle of limited cell numbers in the restoration process, and dedifferentiation is a prerequisite. Gallic acid (GA) has been used in the treatment of arthritis, but its biocompatibility is inferior to that of other compounds. In this study, we modified GA by incorporating sulfamonomethoxine sodium and synthesized a sulfonamido-based gallate, JJYMD-C, and evaluated its effect on chondrocyte metabolism. Our results showed that JJYMD-C could effectively increase the levels of the collagen II, Sox9, and aggrecan genes, promote chondrocyte growth, and enhance secretion and synthesis of cartilage extracellular matrix. On the other hand, expression of the collagen I gene was effectively down-regulated, demonstrating inhibition of chondrocyte dedifferentiation by JJYMD-C. Hypertrophy, as a characteristic of chondrocyte ossification, was undetectable in the JJYMD-C groups. We used JJYMD-C at doses of 0.125, 0.25, and 0.5 µg/mL, and the strongest response was observed with 0.25 µg/mL. This study provides a basis for further studies on a novel agent in the treatment of articular cartilage defects.
Descritores: Benzamidas/síntese química
Desdiferenciação Celular/efeitos dos fármacos
Proliferação de Células/efeitos dos fármacos
Condrócitos/efeitos dos fármacos
Fenótipo
Pirimidinas/síntese química
-Agrecanas/genética
Agrecanas/metabolismo
Anti-Infecciosos/química
Anti-Infecciosos/farmacologia
Benzamidas/farmacologia
Sobrevivência Celular
Desdiferenciação Celular/imunologia
Condrócitos/citologia
Condrócitos/metabolismo
Condrogênese/efeitos dos fármacos
Colágeno Tipo I/genética
Colágeno Tipo I/metabolismo
Colágeno Tipo II/genética
Colágeno Tipo II/metabolismo
Glicosaminoglicanos/análise
Imuno-Histoquímica
Citometria de Varredura a Laser
Cultura Primária de Células
Pirimidinas/farmacologia
Reação em Cadeia da Polimerase em Tempo Real
Fatores de Transcrição SOX9/genética
Fatores de Transcrição SOX9/metabolismo
Engenharia Tecidual
Limites: Animais
Coelhos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-712970
Autor: Parra, E.R.; Pincelli, M.S.; Teodoro, W.R.; Velosa, A.P.P.; Martins, V.; Rangel, M.P.; Barbas-Filho, J.V.; Capelozzi, V.L..
Título: Modeling pulmonary fibrosis by abnormal expression of telomerase/apoptosis/collagen V in experimental usual interstitial pneumonia
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(7):567-575, 07/2014. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Limitations on tissue proliferation capacity determined by telomerase/apoptosis balance have been implicated in pathogenesis of idiopathic pulmonary fibrosis. In addition, collagen V shows promise as an inductor of apoptosis. We evaluated the quantitative relationship between the telomerase/apoptosis index, collagen V synthesis, and epithelial/fibroblast replication in mice exposed to butylated hydroxytoluene (BHT) at high oxygen concentration. Two groups of mice were analyzed: 20 mice received BHT, and 10 control mice received corn oil. Telomerase expression, apoptosis, collagen I, III, and V fibers, and hydroxyproline were evaluated by immunohistochemistry, in situ detection of apoptosis, electron microscopy, immunofluorescence, and histomorphometry. Electron microscopy confirmed the presence of increased alveolar epithelial cells type 1 (AEC1) in apoptosis. Immunostaining showed increased nuclear expression of telomerase in AEC type 2 (AEC2) between normal and chronic scarring areas of usual interstitial pneumonia (UIP). Control lungs and normal areas from UIP lungs showed weak green birefringence of type I and III collagens in the alveolar wall and type V collagen in the basement membrane of alveolar capillaries. The increase in collagen V was greater than collagens I and III in scarring areas of UIP. A significant direct association was found between collagen V and AEC2 apoptosis. We concluded that telomerase, collagen V fiber density, and apoptosis evaluation in experimental UIP offers the potential to control reepithelization of alveolar septa and fibroblast proliferation. Strategies aimed at preventing high rates of collagen V synthesis, or local responses to high rates of cell apoptosis, may have a significant impact in pulmonary fibrosis.
Descritores: Apoptose/fisiologia
Colágeno Tipo V/biossíntese
Fibrose Pulmonar Idiopática/patologia
Fibrose Pulmonar/patologia
Telomerase/metabolismo
-Hidroxitolueno Butilado
Proliferação de Células
Colágeno Tipo I/análise
Colágeno Tipo II/análise
Colágeno Tipo V/análise
Modelos Animais de Doenças
Células Epiteliais/metabolismo
Células Epiteliais/patologia
Imunofluorescência
Fibroblastos/metabolismo
Fibroblastos/patologia
Hidroxiprolina/análise
Imuno-Histoquímica
Marcação In Situ das Extremidades Cortadas
Camundongos Endogâmicos BALB C
Microscopia Eletrônica
Alvéolos Pulmonares/patologia
Alvéolos Pulmonares/ultraestrutura
Coloração e Rotulagem
Telomerase/isolamento & purificação
Limites: Animais
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-709871
Autor: Souza, Maria V de; Pinto, José de O; Costa, Marcela B. M da; Alves, Murilo S; Silva, Micheline O. da; Martinho, Karina O; Fietto, Luciano G.
Título: Expressão gênica do colágeno em ferida cutânea de equinos tratada com plasma rico em plaquetas / Collagen gene expression in skin wound of horses treated with platelet-rich plasma
Fonte: Pesqui. vet. bras = Braz. j. vet. res;34(3):233-240, mar. 2014. graf.
Idioma: pt.
Resumo: [...]The objective of this study was to evaluate type I and III collagen gene expression during different phases of the healing process of PRP-treated skin. Eight healthy crossbred geldings, aged 16 and 17 years (16.37±0.52) were used. Three quadrangular-shaped lesions (6.25cm²) were surgically induced in the right and left gluteal regions of all the animals. Twelve hours after induction of the lesions, 0.5mL of PRP was administered in each of the four edges of the wounds (T=treated group) in one of the gluteal regions, randomly chosen. The contralateral region was used as control (NT=non-treated group). The wounds were submitted to daily cleaning with Milli-Q water, and the samples were obtained with a 6mm diameter biopsy Punch. Six skin biopsies were obtained, with the first being performed on the day the lesions were induced (T0), and the others 1 (T1), 2 (T2), 7 (T3), and 14 (T4) days, after the wound was induced. The sixth biopsy (T5) was performed after fully healed of the skin. Evaluation of type I and III collagen gene expression was carried out by the qRT-PCR technique. The data were analyzed by the Bonferroni test, Student t-test, paired t-test, and regression analysis (p<0,05). Difference (p<0.05) between groups were observed for both collagen gene expressions from T1 to T4, being higher in the animals of group T. The peak for type I and III collagen gene expressions occurred in T5 for both groups, but the highest expression was different (p<0.05) from zero time, starting in T3. In the animals of treated group, collagen expression started to establish at T5, while in the horses of NT group, the values remained increased. Local administration of a single PRP dose in cutaneous wound of the gluteal region of horses results in a higher local gene expression of type I and III collagens. However, this expression does not alter the maximum time of macroscopic healing of the wound.

[...] Objetivou-se avaliar a expressão dos genes dos colágenos tipos I e III durante diferentes fases do processo de cicatrização da pele tratada com PRP. Foram utilizados oito equinos machos castrados, mestiços, hígidos, com idade entre 16 e 17 (16,37±0,52) anos. Três feridas em formato quadrangular (6,25cm²) foram confeccionadas nas regiões glúteas direita e esquerda de todos os animais. Doze horas após indução das lesões, 0,5mL do PRP foi administrado em cada uma das quatro extremidades das feridas (T=grupo tratado), de uma das regiões glúteas, escolhida aleatoriamente. A região contralateral foi utilizada como controle (NT=grupo não tratado). As feridas foram submetidas à limpeza diária com água Milli Q, e amostras foram obtidas com biópsias utilizando-se Punch de 6mm de diâmetro. Seis biópsias de pele foram obtidas a primeira no dia de indução das lesões (T0), e as demais com 1 (T1) 2 (T2) 7 (T3) e 14 (T4) dias após a realização das feridas. A sexta biópsia (T5) foi realizada após o completo fechamento da pele. A avaliação da expressão dos genes dos colágenos tipos I e III foi realizada pela técnica qRT-PCR e os dados analisados pelo teste de Bonferroni, t de Student, t pareado e análise de regressão (p<0,05). Diferenças (p<0,05), entre grupos, foram observadas para a expressão de ambos os colágenos nos T1 a T4, sendo maior nos animais do grupo T. O pico de expressão dos colágenos tipos I e III ocorreu no T5 para ambos os grupos, mas a maior expressão foi diferente (p<0,05) do tempo zero a partir do T3. Nos animais do grupo tratado a expressão dos colágenos começou a estabilizar no T5, enquanto que nos equinos do NT os valores permaneceram elevados. A administração local de uma única dose do PRP em ferida cutânea na região glútea de equinos, resulta em maior expressão gênica local dos colágenos tipos I e III. Entretanto, essa expressão não altera o tempo máximo de fechamento macroscópico da ferida.
Descritores: Ativação Plaquetária
Colágeno Tipo I
Colágeno Tipo II
Cicatrização/fisiologia
Expressão Gênica
Cavalos
Plasma Rico em Plaquetas
-Curativos Oclusivos/veterinária
Reação em Cadeia da Polimerase/veterinária
Limites: Animais
Masculino
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-705770
Autor: Wang, Z.H.; Li, X.L.; He, X.J.; Wu, B.J.; Xu, M.; Chang, H.M.; Zhang, X.H.; Xing, Z.; Jing, X.H.; Kong, D.M.; Kou, X.H.; Yang, Y.Y..
Título: Delivery of the Sox9 gene promotes chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells in an in vitro model
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(4):279-286, 8/4/2014. tab, graf.
Idioma: en.
Projeto: the Natural Science Foundation of China; . the Second Hospital of Xi'an Jiaotong University.
Resumo: SRY-related high-mobility-group box 9 (Sox9) gene is a cartilage-specific transcription factor that plays essential roles in chondrocyte differentiation and cartilage formation. The aim of this study was to investigate the feasibility of genetic delivery of Sox9 to enhance chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells (hUC-MSCs). After they were isolated from human umbilical cord blood within 24 h after delivery of neonates, hUC-MSCs were untreated or transfected with a human Sox9-expressing plasmid or an empty vector. The cells were assessed for morphology and chondrogenic differentiation. The isolated cells with a fibroblast-like morphology in monolayer culture were positive for the MSC markers CD44, CD105, CD73, and CD90, but negative for the differentiation markers CD34, CD45, CD19, CD14, or major histocompatibility complex class II. Sox9 overexpression induced accumulation of sulfated proteoglycans, without altering the cellular morphology. Immunocytochemistry demonstrated that genetic delivery of Sox9 markedly enhanced the expression of aggrecan and type II collagen in hUC-MSCs compared with empty vector-transfected counterparts. Reverse transcription-polymerase chain reaction analysis further confirmed the elevation of aggrecan and type II collagen at the mRNA level in Sox9-transfected cells. Taken together, short-term Sox9 overexpression facilitates chondrogenesis of hUC-MSCs and may thus have potential implications in cartilage tissue engineering.
Descritores: Diferenciação Celular/genética
Condrogênese/genética
Sangue Fetal/citologia
Células-Tronco Mesenquimais/citologia
Fatores de Transcrição SOX9/genética
-Agrecanas/biossíntese
Western Blotting
Cartilagem/metabolismo
Proliferação de Células/genética
Condrócitos/metabolismo
Colágeno Tipo II/biossíntese
Citometria de Fluxo
Proteínas de Fluorescência Verde
Regulação da Expressão Gênica/fisiologia
Células Endoteliais da Veia Umbilical Humana/citologia
Imuno-Histoquímica
Imunofenotipagem
Cultura Primária de Células
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Engenharia Tecidual
Transfecção
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-692091
Autor: Torres, Elenilde Maria dos Santos.
Título: Ação in vitro da insulina sobre a proliferação e a maturação condrocítica em cultura de micromassa de alta densidade / In vitro action of insulin on the proliferation and maturation in culture condrocítica high density micromass.
Fonte: Niterói; s.n; 2000. [86] p. ilus.
Idioma: pt.
Tese: Apresentada a Universidade Federal Fluminense. Doutorado em Patologia Experimental para obtenção do grau de Doutor.
Resumo: Vários fatores de crescimento e hormônios influenciam diretamente a maturação cartilaginosa. Na investigação da influência de moléculas sinalizadoras da condrogênese, da maturação e da calcificação cartilaginosa é necessária a manutenção da interação tri-dimensional entre células e matriz...Embora a apoptose tenha sido observada em todas as culturas a partir do dia 7, o número de células apoptóticas aumentou em função do tempo em cultura nas células suplementadas com SFB a 10%. Nas culturas tratadas com insulina o número de células marcadas pela técnica do TUNEL e a produção de colágeno tipo II foi significativamente reduzida. Sob a influência da insulina, as células cartilaginosas mantêm seu potencial proliferativo e não se transformam em condrócitos hipertróficos típicos, sugerindo que a insulina tenha uma função preponderante na regulação da maturação e hipertrofia condrocítica através de um possível efeito anti-apoptótico.
Descritores: Apoptose
Desenvolvimento Ósseo
Condrogênese
Colágeno Tipo II
Insulina
Patologia
Antígeno Nuclear de Célula em Proliferação
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM
BR408.1; T615.365, T693, 2000


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Texto completo SciELO Brasil
Texto completo
Id: lil-674153
Autor: Sotoudeh, Amir; Jahanshahi, Amirali; Takhtfooladi, Mohammad Ashrafzadeh; Bazazan, Ali; Ganjali, Amin; Harati, Maryam Pourramezani.
Título: Study on nano-structured hydroxyapatite/zirconia stabilized yttria on healing of articular cartilage defect in rabbit
Fonte: Acta cir. bras;28(5):340-345, May 2013. ilus, tab.
Idioma: en.
Resumo: PURPOSE: Articular Cartilage has limited potential for self-repair and tissue engineering approaches attempt to repair articular cartilage by scaffolds. We hypothesized that the combined hydroxyapatite and zirconia stabilized yttria would enhance the quality of cartilage healing. METHODS: In ten New Zealand white rabbits bilateral full-thickness osteochondral defect, 4 mm in diameter and 3 mm depth, was created on the articular cartilage of the patellar groove of the distal femur. In group I the scaffold was implanted into the right stifle and the same defect was created in the left stifle without any transplant (group II). Specimens were harvested at 12 weeks after implantation, examined histologically for morphologic features, and stained immunohistochemically for type-II collagen. RESULTS: In group I the defect was filled with a white translucent cartilage tissue In contrast, the defects in the group II remained almost empty. In the group I, the defects were mostly filled with hyaline-like cartilage evidenced but defects in group II were filled with fibrous tissue with surface irregularities. Positive immunohistochemical staining of type-II collagen was observed in group I and it was absent in the control group. CONCLUSION: The hydroxyapatite/yttria stabilized zirconia scaffold would be an effective scaffold for cartilage tissue engineering.
Descritores: Materiais Biocompatíveis/uso terapêutico
Cartilagem Articular/lesões
Durapatita/uso terapêutico
Nanoestruturas/uso terapêutico
Cicatrização/efeitos dos fármacos
Ítrio/uso terapêutico
Zircônio/uso terapêutico
-Colágeno Tipo II/análise
Teste de Materiais
Reprodutibilidade dos Testes
Regeneração/efeitos dos fármacos
Propriedades de Superfície
Fatores de Tempo
Resultado do Tratamento
Limites: Animais
Masculino
Coelhos
Responsável: BR1.1 - BIREME



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