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Id: biblio-1055084
Autor: Brianezi, Ledimar; Ornelas, Elisabete; Gehrke, Flávia de Sousa; Fonseca, Fernando Luiz Affonso; Alves, Beatriz da Costa Aguiar; Sousa, Luiz Vinicius de Alcantara; Souza, Jessica; Maifrino, Laura Beatriz Mesiano.
Título: Effects of Physical Training on the Myocardium of Oxariectomized LDLr Knockout Mice: MMP 2/9, Collagen I/III, Inflammation and Oxidative Stress / Efeitos do Treinamento Físico sobre o Miocárdio de Camundongos LDLr Knockout Ovariectomizadas: MMP-2 e -9, Colágeno I/III, Inflamação e Estresse Oxidativo
Fonte: Arq. bras. cardiol;114(1):100-105, Jan. 2020. tab, graf.
Idioma: en.
Resumo: Abstract Background: The emergence of coronary heart disease is increased with menopause, physical inactivity and with dyslipidemia. Physical training is known to promote the improvement of cardiovascular functions. Objective: To investigate the effects of aerobic physical training on the left ventricle in ovariectomized LDL knockout mice. Methods: Thirty animals were divided into 6 groups (n = 5): Sedentary non-ovariectomized control; Sedentary ovariectomized control; Trained ovariectomized control; Sedentary non-ovariectomized LDL-knockout, sedentary ovariectomized LDL-knockout and trained ovariectomized LDL-knockout. We analyzed the average parameters of apparent density of collagen fibers types I and III, and metalloproteinase type 2 and type 9, were considered significant p < 0.05. Results: The results showed that the proposed exercise protocol altered the volume of type I collagen fibers, altered collagen remodeling parameters (MMP-2), and also reduced the 8-hydroxy-2'-deoxyguanosine (8OHdG) oxidative stress parameter. Conclusion: Moderate intensity aerobic training acts on collagen fiber volume, on collagen remodeling with the reduction of oxidative stress in the left ventricles of ovariectomized LDL-knockout mice.

Resumo Fundamento: O surgimento da doença cardíaca coronariana aumenta com a menopausa, inatividade física e dislipidemia. Sabe-se que o treinamento físico promove a melhora das funções cardiovasculares Objectivo: Investigar os efeitos do treinamento físico aeróbico sobre o ventrículo esquerdo em camundongos LDL knockout ovariectomizadas. Métodos: Trinta animais foram divididos em 6 grupos (n = 5): controle sedentário não ovariectomizado, controle sedentário ovariectomizado, controle treinado ovariectomizado, sedentário LDL-knockout não ovariectomizado, sedentário LDL-knockout ovariectomizado e treinado LDL-knockout ovariectomizado. Analisamos os parâmetros médios da densidade de volume de fibras colágenas tipo I e III, e metaloproteinases 2 e 9. Valores de p < 0,05 foram considerados significativos. Resultados: Os resultados mostram que o protocolo de exercício proposto alterou o volume de fibras colágenas do tipo I e os parâmetros de remodelamento do colágeno (MMP-2), e ainda reduziu o parâmetro de estresse oxidativo do 8-hidroxi-2'-deoxiganosina (8-OhdG). Conclusão: O treinamento aeróbico de intensidade moderada age sobre o volume das fibras colágenas e sobre o remodelamento de colágeno, com redução do estresse oxidativo em ventrículos esquerdos de camundongos ovariectomizados LDLr Knockout.
Descritores: Metaloproteinase 2 da Matriz/metabolismo
Metaloproteinase 9 da Matriz/metabolismo
Colágeno Tipo I/metabolismo
Colágeno Tipo III/metabolismo
Inflamação/fisiopatologia
Miocárdio/metabolismo
-Condicionamento Físico Animal/fisiologia
Imuno-Histoquímica
Ovariectomia
Camundongos Knockout
Estresse Oxidativo/fisiologia
Modelos Animais
Limites: Animais
Feminino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-1054681
Autor: Nascimento, Lízia Daniela e Silva; Nicolau, Renata Amadei; Maia Filho, Antônio Luiz Martins; Santos, José Zilton Lima Verde; Fonseca, Khetyma Moreira; Ferreira, Danniel Cabral Leão; Sousa, Rayssilane Cardoso de; Viana, Vicente Galber Freitas; Carvalho, Luiz Fernando Meneses; Figueredo-Silva, José.
Título: Effect of norbixin-based poly(hydroxybutyrate) membranes on the tendon repair process after tenotomy in rats
Fonte: Acta cir. bras;34(11):e201901101, Nov. 2019. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To determine the efficacy of norbixin-based poly(hydroxybutyrate) (PHB) membranes for Achilles tendon repair. Methods: Thirty rats were submitted to total tenotomy surgery of the right Achilles tendon and divided into two groups (control and membrane; n = 15 each), which were further subdivided into three subgroups (days 7, 14, and 21; n = 5 each). Samples were analyzed histologically. Results: Histological analysis showed a significant reduction in inflammatory infiltrates on days 7, 14 (p < 0.0001 for both), and 21 (p = 0.0004) in the membrane group compared to that in the control group. There was also a significant decrease in the number of fibroblasts in the control group on days 7, 14 (p < 0.0001), and 21 (p = 0.0032). Further, an increase in type I collagen deposition was observed in the membrane group compared to that in the control group on days 7 (p = 0.0133) and 14 (p = 0.0107). Conclusion: Treatment with norbixin-based PHB membranes reduces the inflammatory response, increases fibroblast proliferation, and improves collagen production in the tendon repair region, especially between days 7 and 14.
Descritores: Poliésteres/farmacologia
Tendão do Calcâneo/cirurgia
Tendão do Calcâneo/efeitos dos fármacos
Carotenoides/farmacologia
Tenotomia/métodos
Hidroxibutiratos/farmacologia
-Valores de Referência
Regeneração/efeitos dos fármacos
Tendão do Calcâneo/patologia
Reprodutibilidade dos Testes
Ratos Wistar
Colágeno Tipo I/análise
Colágeno Tipo I/efeitos dos fármacos
Colágeno Tipo III/análise
Colágeno Tipo III/efeitos dos fármacos
Fibroblastos/efeitos dos fármacos
Limites: Humanos
Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-886256
Autor: Campelo, Mariana Barbosa Dias; Santos, Joelita de Alencar Fonseca; Maia Filho, Antonio Luiz Martins; Ferreira, Daniel Cabral Leão; Sant'Anna, Luciana Barros; Oliveira, Rauirys Alencar de; Maia, Leonardo Fonseca; Arisawa, Emilia Ângela Loschiavo.
Título: Effects of the application of the amniotic membrane in the healing process of skin wounds in rats
Fonte: Acta cir. bras;33(2):144-155, Feb. 2018. graf.
Idioma: en.
Resumo: Abstract Purpose: To evaluate the efficacy of the application of the human amniotic membrane (HAM) on the inflammatory process, fibroblast proliferation, formation of collagenand reduction of skin wound areas in rats. Methods: Thirty six rats were submitted to a surgical injury induction and divided into two groups (n = 18): group C (control) and T (treated with the HAM). The macroscopic evolution in the wound area and the histological characteristics of the skin samples were evaluated. Results: The regression of the wound area was greater in group T. The histological analysis revealed a significant reduction (p < 0.05) in the inflammatory infiltrate in group T at all experimental periods compared with that in the control group. Furthermore, the group T presented a significant increase in the proliferation of fibroblasts at 14 and 21 days compared with group C (p < 0.05). Regarding the deposition of mature collagen fibers, there was an increase in the replacement of type III collagen by type I collagen in group T (p < 0.05). Conclusion: Treatment with the HAM reduced the healing time as well as the inflammatory responses, increased the proliferation of fibroblasts, and induced a higher concentration of mature collagen fibers.
Descritores: Pele/lesões
Cicatrização/fisiologia
Curativos Biológicos
Colágeno/farmacologia
Âmnio/transplante
-Pele/patologia
Cicatrização/efeitos dos fármacos
Distribuição Aleatória
Ratos Wistar
Colágeno Tipo I/metabolismo
Colágeno Tipo I/farmacologia
Colágeno Tipo III/metabolismo
Colágeno Tipo III/farmacologia
Modelos Animais de Doenças
Fibroblastos/metabolismo
Fibroblastos/patologia
Âmnio/química
Inflamação/metabolismo
Limites: Humanos
Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-886197
Autor: Zhang, Chong; Liu, Yu-Jie.
Título: Biomechanic and histologic analysis of fibroblastic effects of tendon to bone healing by transforming growth factor ß1 (TGF-ß1) in rotator cuff tears
Fonte: Acta cir. bras;32(12):1045-1055, Dec. 2017. graf.
Idioma: en.
Projeto: Hebei Province Science and Technology Project.
Resumo: Abstract Purpose: To evaluate the effect of transforming growth factor β1 (TGF-β1) on tendon-to-bone reconstruction of rotator cuff tears. Methods: Seventy-two rat supraspinatus tendons were transected and reconstructed in situ. At 8 and 16 weeks, specimens of three groups; that is control, L-dose (low dose), and H-dose (high dose) were harvested and underwent a biomechanical test to evaluate the maximum load and stiffness values. Histology sections of the tendon-to-bone interface were identified by hematoxylin-eosin or Masson trichrome stain. Collagen type III was observed by picric acid sirius red staining under polarized light. The level of insulin-like growth factor 1 (IGF-1) and vascular endothelial growth factor (VEGF) was measured by the enzyme-linked immunosorbent assay (ELISA) method. Results: Collagen type III of the H-dose group had a significant difference in histology structure compared with the L-dose group (P<0.05). The maximum load and stiffness decreased significantly in the control group compared with the values of the L-dose and H-dose groups. The stiffness among the three groups differed significantly at the same postoperative time (P<0.05). Interestingly, progressive reestablishment of collagen type III affected tendon-to-bone healing significantly in the later stages. Conclusion: The H-dose was associated with an increased collagen type III morphology stimulated by TGF-β1.
Descritores: Traumatismos dos Tendões/tratamento farmacológico
Cicatrização/fisiologia
Manguito Rotador/cirurgia
Fatores de Crescimento do Endotélio Vascular/metabolismo
Fator de Crescimento Transformador beta1/metabolismo
Lesões do Manguito Rotador/cirurgia
-Traumatismos dos Tendões/metabolismo
Resistência à Tração/fisiologia
Cicatrização/efeitos dos fármacos
Fenômenos Biomecânicos
Ensaio de Imunoadsorção Enzimática
Manguito Rotador/metabolismo
Ratos Sprague-Dawley
Colágeno Tipo III/metabolismo
Modelos Animais de Doenças
Elasticidade/fisiologia
Fator de Crescimento Transformador beta1/farmacologia
Força Muscular/fisiologia
Fibroblastos/efeitos dos fármacos
Fibroblastos/fisiologia
Lesões do Manguito Rotador/metabolismo
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-973480
Autor: Chia, Chang Yung; Medeiros, Andréia Dantas; Corraes, André de Menezes Silva; Manso, José Eduardo Ferreira; Silva, César Silveira Claudio da; Takiya, Christina Maeda; Vanz, Ricardo Luís.
Título: Healing effect of andiroba-based emulsion in cutaneous wound healing via modulation of inflammation and transforming growth factor beta 3
Fonte: Acta cir. bras;33(11):1000-1015, Nov. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To evaluate the effects and mechanisms of andiroba-based emulsion (ABE) topical treatment on full-thickness cutaneous wounds in rats. Methods: The wounds were harvested on days 3, 7, 15, and 20 post-surgery. Wound contraction rate, quantitative immunohistochemistry [macrophages, myofibroblasts, capillaries, collagens (col) I and III, transforming growth factor β3β (TGFβ3)], and tensile strength were assessed. Results: Treated wounds were smaller, contracted earlier and had increased angiogenesis, fewer CD68+ and M2 macrophages on days 7 and 15, but higher on day 20. Myofibroblasts appeared on days 3 to 7 in untreated wounds and on days 7 to 15 in treated wounds. TGFβ3 levels were higher in the treated wounds, less dense collagen fibers, lower col I/III ratios and a higher tensile strength. Conclusion: These results demonstrate the important anti-inflammatory role of treatment and the associated modulation of macrophages, myofibroblasts, and TGFβ3 levels. Collagen fibers in the treated wounds were more organized and less dense, similar to unwounded skin, which likely contributed to the higher tensile strength.
Descritores: Pele/efeitos dos fármacos
Cicatrização/efeitos dos fármacos
Óleos Vegetais/farmacologia
Meliaceae/química
Fator de Crescimento Transformador beta3/efeitos dos fármacos
Anti-Inflamatórios/farmacologia
-Pele/patologia
Administração Cutânea
Imuno-Histoquímica
Reprodutibilidade dos Testes
Resultado do Tratamento
Ratos Wistar
Colágeno Tipo I/análise
Colágeno Tipo III/análise
Emulsões
Matriz Extracelular/efeitos dos fármacos
Fator de Crescimento Transformador beta3/análise
Miofibroblastos/efeitos dos fármacos
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-838060
Autor: Torii, Kazuhiro; Maeshige, Noriaki; Aoyama-Ishikawa, Michiko; Miyoshi, Makoto; Terashi, Hiroto; Usami, Makoto.
Título: Combination therapy with butyrate and docosahexaenoic acid for keloid fibrogenesis: an in vitro study
Fonte: An. bras. dermatol;92(2):184-190, Mar.-Apr. 2017. tab, graf.
Idioma: en.
Projeto: JSPS KAKENHI.
Resumo: Abstract: Background: A single, effective therapeutic regimen for keloids has not been established yet, and the development of novel therapeutic approaches is expected. Butyrate, a short-chain fatty acid, and docosahexaenoic acid (DHA), a ω-3 polyunsaturated fatty acid, play multiple anti-inflammatory and anticancer roles via their respective mechanisms of action. Objective: In this study, we evaluated the antifibrogenic effects of their single and combined use on keloid fibroblasts. Methods: Keloid fibroblasts were treated with butyrate (0-16 mM) and/or DHA (0-100 µM) for 48 or 96 h. Results: Butyrate inhibited cell proliferation, and α-smooth muscle actin (α-SMA) and type III collagen expressions, with inhibition of the transforming growth factor (TGF)-β1 and TGF-β type I receptor expressions and increased prostaglandin E2 with upregulation of cyclooxygenase-1 expression with induction of histone acetylation. DHA inhibited α-SMA, type III collagen, and TGF-β type I receptor expressions. Then, the butyrate/DHA combination augmented the antifibrogenic effects, resulting in additional inhibition of α-SMA, type I and III collagen expressions, with strong disruption of stress fiber and apoptosis induction. Moreover, the butyrate/DHA combination inhibited the cyclooxygenase-2 expression, suggesting stronger anti-inflammatory effect than each monotherapy. Study limitations: Activation in keloid tissue is affected not only by fibroblasts but also by epithelial cells and immune cells. Evaluation of the effects by butyrate and DHA in these cells or in an in vivo study is required. Conclusion: This study demonstrated that butyrate and docosahexaenoic acid have antifibrogenic effects on keloid fibroblasts and that these may exert therapeutic effects for keloid.
Descritores: Butiratos/uso terapêutico
Ácidos Docosa-Hexaenoicos/uso terapêutico
Fibroblastos
Queloide/tratamento farmacológico
-Células Cultivadas
Proteínas Serina-Treonina Quinases
Receptores de Fatores de Crescimento Transformadores beta
Terapia Combinada
Colágeno Tipo I
Colágeno Tipo III
Proliferação de Células
Limites: Humanos
Responsável: BR1.1 - BIREME


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Czeczko, Nicolau Gregori
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Id: biblio-837658
Autor: Silva, Eduardo Nascimento; Ribas-Filho, Jurandir Marcondes; Czeczko, Nicolau Gregori; Pachnicki, Jan Pawel Andrade; Netto, Mário Rodrigues Montemor; Lipinski, Leandro Cavalcante; Noronha, Lucia de; Colman, Joelmir; Zeni, João Otavio; Carvalho, Caroline Aragão de.
Título: Histological evaluation of capsules formed by silicon implants coated with polyurethane foam and with a textured surface in rats
Fonte: Acta cir. bras;31(12):774-782, Dec. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT PURPOSE: To assess the capsules formed by silicone implants coated with polyurethane foam and with a textured surface. METHODS: Sixty-four Wistar albinus rats were divided into two groups of 32 each using polyurethane foam and textured surface. The capsules around the implants were analyzed for 30, 50, 70 and 90 days. Were analyzed the following parameters: foreign body reaction, granulation tissue, presence of myofibroblasts, neoangiogenesis, presence of synovial metaplasia, capsular thickness, total area and collagen percentage of type I and III, in capsules formed around silicone implants in both groups. RESULTS: The foreign body reaction was only present in the four polyurethane subgroups. The formation of granulation tissue and the presence of myofibroblasts were higher in the four polyurethane subgroups. Regarding to neoangiogenesis and synovial metaplasia, there was no statistical difference between the groups. Polyurethane group presented (all subgroups) a greater capsule thickness, a smaller total area and collagen percentage of type I and a higher percentage area of type III, with statistical difference. CONCLUSION: The use of polyurethane-coated implants should be stimulated by the long-term results in a more stable capsule and a lower incidence of capsular contracture, despite developing a more intense and delayed inflammatory reaction in relation to implants with textured surface.
Descritores: Poliuretanos/administração & dosagem
Reação a Corpo Estranho/patologia
Implantes de Mama/efeitos adversos
Géis de Silicone/administração & dosagem
Materiais Revestidos Biocompatíveis
Contratura Capsular em Implantes/patologia
-Propriedades de Superfície
Ratos Wistar
Implante Mamário/métodos
Colágeno Tipo I/análise
Colágeno Tipo III/análise
Limites: Animais
Feminino
Ratos
Responsável: BR1.1 - BIREME


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Id: lil-783797
Autor: Biondo-Simões, Maria de Lourdes Pessole; Morais, Camila Gomes de; Tocchio, Anna Flávia Zonato; Miranda, Renata Augusta de; Moura, Paula Almeida Pamponet; Colla, Kelly; Robes, Rogério Ribeiro; Ioshii, Sérgio Ossamu; Tomasich, Flávio Daniel Saavedra.
Título: Characteristics of the fibroplasia and collagen expression in the abdominal wall after implant of the polypropylene mesh and polypropylene/polyglecaprone mesh in rats
Fonte: Acta cir. bras;31(5):294-299, May 2016. graf.
Idioma: en.
Resumo: ABSTRACT PURPOSE: To compare fibroplasia and the resistance of the abdominal wall when polypropylene meshes and polypropylene/poliglecaprone are used. METHODS: Seventy-seven male Wistar rats were divided into three groups: Control Group (for resistance); Group E (polypropylene mesh); and Group U (polypropylene/poliglecaprone mesh). The animals in Groups E and U had a standard muscular and aponeurotic defect, with integral peritoneum, and correction with the mesh. Measurements were taken 4, 7, 14, 28 and 56 days after surgery. The resistance, and collagen density were studied. RESULTS: Resistance on the 56th day was similar in both meshes. The gain in resistance described an ascending curve for the polypropylene mesh and was irregular in the case of the polypropylene/poliglecaprone. Fibroplasia showed a gain in type I and type III collagen in both groups (p<0.001). Collagen III stabilized in the 14th day and collagen I continued to ascend. CONCLUSIONS: The gain in resistance of the polypropylene mesh is regular and ascending, whereas the polypropylene/poliglecaprone is not regular. The final resistance of both meshes is similar; the collagen density increases over time, and show the same inflammatory potential.
Descritores: Poliésteres/uso terapêutico
Polipropilenos/uso terapêutico
Colágeno/metabolismo
Parede Abdominal/cirurgia
Dioxanos/uso terapêutico
Hérnia Incisional/cirurgia
-Período Pós-Operatório
Resistência à Tração/fisiologia
Teste de Materiais/instrumentação
Ratos Wistar
Modelos Animais
Colágeno Tipo I/metabolismo
Colágeno Tipo III/metabolismo
Hérnia Incisional/fisiopatologia
Hérnia Incisional/metabolismo
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Malafaia, Osvaldo
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Id: lil-783801
Autor: Sousa, Eros Luiz de; Wouk, Antônio Felipe Paulino de Figueiredo; Malafaia, Osvaldo; Ribas-Filho, Jurandir Marcondes; Noronha, Lucia; Cirio, Silvana Maris; PimpãoVII, Claudia Turra; Marinho Júnior, Carlos Hespanha; Torres, Maria Fernanda; Graça, Yorgos Luiz Santos de Salles; Ferrarin, Daniel Dantas.
Título: Immediate postoperative evaluation of enteric anastomosis after the use of meloxicam in rats
Fonte: Acta cir. bras;31(5):320-326, May 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT PURPOSE : To compare ileal anastomoses in the immediate postoperative healing period after meloxicam use. METHODS: Forty two male Wistar rats were randomly divided into two groups of 21, COX and control group. To COX meloxicam in combination with morphine was given in 3 days period. Control group received only morphine during the same period. Each group was divided into three sub-groups of 7, which were euthanized at 5, 10, and 21 days postoperatively. Comparison was based in histological evaluation of collagen type I and III using sirius red, immunohistochemical through vascular endothelial growth factor and matrix metalloproteinase-9. RESULTS: Healing process in scheduled periods did not show significant differences (p>0.05) between the COX and control groups during any of the periods. CONCLUSION: The use of meloxicam in the postoperative period following ileal anastomosis did not affect healing.
Descritores: Tiazinas/farmacologia
Tiazóis/farmacologia
Cicatrização/efeitos dos fármacos
Anti-Inflamatórios não Esteroides/farmacologia
Inibidores de Ciclo-Oxigenase/farmacologia
Íleo/cirurgia
-Período Pós-Operatório
Fatores de Tempo
Anastomose Cirúrgica
Distribuição Aleatória
Ratos Wistar
Neovascularização Fisiológica/efeitos dos fármacos
Metaloproteinase 9 da Matriz/efeitos dos fármacos
Metaloproteinase 9 da Matriz/metabolismo
Modelos Animais
Colágeno Tipo I/metabolismo
Colágeno Tipo III/metabolismo
Receptores de Fatores de Crescimento do Endotélio Vascular/efeitos dos fármacos
Receptores de Fatores de Crescimento do Endotélio Vascular/metabolismo
Íleo/irrigação sanguínea
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: biblio-837705
Autor: Jia, Peng; Hu, Yu; Li, Gang; Sun, Yuqin; Zhao, Jian; Fu, Jie; Lu, Cuixia; Liu, Bin.
Título: Roles of the ERK1/2 and PI3K/PKB signaling pathways in regulating the expression of extracellular matrix genes in rat pulmonary artery smooth muscle cells
Fonte: Acta cir. bras;32(5):350-358, May 2017. tab, graf.
Idioma: en.
Projeto: Science and Technology Research Foundation of Sichuan Provincial Health Department.
Resumo: Abstract Purpose: To investigate the mechanisms by which PD98059 and LY294002 interfere with the abnormal deposition of extracellular matrix regulated by connective tissue growth factor (CTGF) of rat pulmonary artery smooth muscle cells (PASMCs). Methods: Rat PASMCs were cultured and separated into a control group. Real-time fluorescence quantitative PCR was performed to detect the expression of collagen III and fibronectin mRNA. Immunohistochemistry and western blot analyses were performed to detect the expression of collagen III protein. Results: The expression of collagen III and fibronectin mRNA was greater in PASMCs stimulated with CTGF for 48 h, than in the control group. After 72h of stimulation, the expression of collagen III protein in the PASMCs was greater than in the control. The equivalent gene and protein expression of the CPL group were much more significant. Conclusions: CTGF can stimulate the gene expression of collagen III and fibronectin in PASMCs, which may be one of the factors that promote pulmonary vascular remodeling (PVR) under the conditions of pulmonary arterial hypertension (PAH). PD98059 and LY294002 can inhibit the ERK1/2 and PI3K/PKB signaling pathways, respectively, thus interfering with the biological effects of CTGF. This may be a new way to reduce PAH-PVR.
Descritores: Flavonoides/farmacologia
Cromonas/farmacologia
Fibronectinas/metabolismo
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Colágeno Tipo III/metabolismo
Fator de Crescimento do Tecido Conjuntivo/farmacologia
-Artéria Pulmonar/citologia
Expressão Gênica/efeitos dos fármacos
Células Cultivadas
Regulação da Expressão Gênica
Fibronectinas/genética
Ratos Sprague-Dawley
Fosfatidilinositol 3-Quinases/metabolismo
Modelos Animais
Colágeno Tipo III/genética
Miócitos de Músculo Liso/citologia
Miócitos de Músculo Liso/metabolismo
Proteínas Proto-Oncogênicas c-akt/metabolismo
Fator de Crescimento do Tecido Conjuntivo/metabolismo
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME



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