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Id: biblio-839351
Autor: An, Chao; Ma, Sai-jian; Chang, Fan; Xue, Wen-jiao.
Título: Efficient production of pullulan by Aureobasidium pullulans grown on mixtures of potato starch hydrolysate and sucrose
Fonte: Braz. j. microbiol;48(1):180-185, Jan.-Mar. 2017. tab, graf.
Idioma: en.
Projeto: Scientific and Technologic Research Program of Shaanxi Academy of Sciences, China; . WesternChinese Academy of Sciences; . Natural Science Basic Research Plan in Shaanxi Province of China; . Shaanxi Science & Technology Co-ordination & Innovation Project; . Science and Technology Program of Xi’an.
Resumo: Abstract Pullulan is a natural exopolysaccharide with many useful characteristics. However, pullulan is more costly than other exopolysaccharides, which limits its effective application. The purpose of this study was to adopt a novel mixed-sugar strategy for maximizing pullulan production, mainly using potato starch hydrolysate as a low-cost substrate for liquid-state fermentation by Aureobasidium pullulans. Based on fermentation kinetics evaluation of pullulan production by A. pullulans 201253, the pullulan production rate of A. pullulans with mixtures of potato starch hydrolysate and sucrose (potato starch hydrolysate:sucrose = 80:20) was 0.212 h−1, which was significantly higher than those of potato starch hydrolysate alone (0.146 h−1) and mixtures of potato starch hydrolysate, glucose, and fructose (potato starch hydrolysate:glucose:fructose = 80:10:10, 0.166 h−1) with 100 g L−1 total carbon source. The results suggest that mixtures of potato starch hydrolysate and sucrose could promote pullulan synthesis and possibly that a small amount of sucrose stimulated the enzyme responsible for pullulan synthesis and promoted effective potato starch hydrolysate conversion effectively. Thus, mixed sugars in potato starch hydrolysate and sucrose fermentation might be a promising alternative for the economical production of pullulan.
Descritores: Ascomicetos/metabolismo
Amido/metabolismo
Sacarose/metabolismo
Solanum tuberosum/química
Fermentação
Glucanos/biossíntese
-Amido/química
Carbono/metabolismo
Cinética
Biomassa
Reatores Biológicos
Técnicas de Cultura Celular por Lotes
Responsável: BR1.1 - BIREME


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Id: biblio-889398
Autor: Lima, Isabela Angeli de; Pomin, Suelen Plaza; Cavalcanti, Osvaldo Albuquerque.
Título: Development and characterization of pullulan-polymethacrylate free films as potential material for enteric drug release
Fonte: Braz. J. Pharm. Sci. (Online);53(3):e00002, 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Free films of pullulan-polymethacrylate associations were produced by casting process to develop a novel target-specific material. For characterization, tests of water vapor permeability, swelling index, infrared absorption spectroscopy, thermogravimetric analysis, scanning electron microscopy and mechanical analysis were performed. The polysaccharide concentration directly influenced vapor permeability and swelling, increasing the values of the latter up to five times when added in a proportion of 20% (per weight). The individual properties of each polymer were maintained, and chemical interactions were not detected. The films were found to be thermally stable and they had unaltered mechanical properties with the addition of the polysaccharide. The microscopic analysis revealed rugosity that was proportional to pullulan and disorganization of the polymer network at pH 6.8. These results suggest that this novel material has potential for enteric drug release because of synergism between pH and enzyme dependence.
Descritores: Comprimidos com Revestimento Entérico/farmacologia
Sistemas de Liberação de Medicamentos/efeitos adversos
-Glucanos
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


  3 / 31 LILACS  
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Baracat, Edmund Chada
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Id: lil-774537
Autor: Matsuoka, Priscila Katsumi; Locali, Rafael Fagionato; Pacetta, Aparecida Maria; Baracat, Edmund Chada; Haddad, Jorge Milhem.
Título: The efficacy and safety of urethral injection therapy for urinary incontinence in women: a systematic review
Fonte: Clinics;71(2):94-100, Feb. 2016. tab, graf.
Idioma: en.
Resumo: To evaluate the efficacy and safety of different bulking agents for treating urinary incontinence in women, a systematic review including only randomized controlled trials was performed. The subjects were women with urinary incontinence. The primary outcomes were clinical and urodynamic parameters. The results were presented as a weighted mean difference for non-continuous variables and as relative risk for continuous variables, both with 95% confidence intervals. Initially, 942 studies were identified. However, only fourteen eligible trials fulfilled the prerequisites. Altogether, the review included 1814 patients in trials of eight different types of bulking agents, and all studies were described and analyzed. The measured outcomes were evaluated using a large variety of instruments. The most common complications of the bulking agents were urinary retention and urinary tract infection. Additionally, there were certain major complications, such as one case of death after use of autologous fat. However, the lack of adequate studies, the heterogeneous populations studied, the wide variety of materials used and the lack of long-term follow-up limit guidance of practice. To determine which substance is the most suitable, there is a need for more randomized clinical trials that compare existing bulking agents based on standardized clinical outcomes.
Descritores: Colágeno/administração & dosagem
Silicones/administração & dosagem
Uretra
Incontinência Urinária/tratamento farmacológico
-Resinas Acrílicas/administração & dosagem
Dimetilpolisiloxanos/administração & dosagem
Glucanos/administração & dosagem
Hidrogéis/administração & dosagem
Injeções/métodos
Ensaios Clínicos Controlados Aleatórios como Assunto
Resultado do Tratamento
Zircônio/administração & dosagem
Limites: Adulto
Feminino
Seres Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: lil-744859
Autor: Rabelo, Doris Firmino; Neri, Anita Liberalesso.
Título: Tipos de configuração familiar e condições de saúde física e psicológica em idosos / Family configuration and physical and psychological health status in a sample of elderly / Tipos de configuración familiar y condiciones de salud física y psicológica en ancianos
Fonte: Cad. saúde pública = Rep. public health;31(4):874-884, 04/2015. tab.
Idioma: pt.
Resumo: Investigamos relações entre configuração familiar (arranjo de moradia, chefia familiar e contribuição financeira para o sustento da família), idade, sexo e condições de saúde física (capacidade funcional, número de doenças e de sinais e sintomas, e envolvimento social) e saúde psicológica (depressão e ansiedade) em idosos, conforme autorrelato. A amostra probabilística tinha 134 idosos sem déficit cognitivo e os dados foram coletados por entrevista domiciliar. Foi feita análise de conglomerados mediante o método da partição (três agrupamentos). As variáveis que mais contribuíram para a formação dos grupos foram as atividades básicas (R2 = 0,732) e instrumentais da vida diária (R2 = 0,487), o número de doenças (R2 = 0,241) e a idade (R2 = 0,225). A configuração familiar predominante foi corresidência com os descendentes, sendo os idosos provedores e chefes de família. Foram encontradas relações entre configuração familiar, condições de saúde física e saúde psicológica. As mulheres apresentaram maior ônus financeiro e pior saúde física e psicológica do que os homens.

This study focused on the relations between family configuration (living arrangements, heads of family, and financial contributions to the family's support), age, gender, and physical health (functional capacity, number of diseases and signs and symptoms, and social involvement) and psychological health (depression and anxiety) among the elderly, based on self-reported data. The probabilistic sample included 134 elderly without cognitive deficit, with data collected in home interviews. Cluster analyses were performed using the partitioning method (three groupings). The variables that contributed the most to forming groups were basic activities of daily living (R2 = 0.732) and instrumental activities of daily living (R2 = 0.487), number of diseases (R2 = 0.241), and age (R2 = 0.225). The predominant family configuration was living with children and/or grandchildren, with the elderly as providers and heads of the family. The study showed associations between family configuration and physical and psychological health status. Women showed a higher financial burden and worse psychological health than men.

Hemos investigado las relaciones entre la configuración familiar (arreglo de la vivienda familiar, liderazgo y contribución financiera para la familia), edad, sexo y condiciones de salud física (capacidad funcional, número de signos y síntomas, enfermedades y participación social) y salud psicológica (depresión y ansiedad) de ancianos, según el mismo informe. La muestra probabilística fue 134 personas mayores sin déficit cognitivo y los datos recogidos por entrevista domiciliaria. Se crearon por el método de análisis de conglomerados de partición (tres grupos). Las variables que han contribuido más a la formación de los grupos fueron actividades básicas (R2 = 0,732) y actividades instrumentales de la vida cotidiana (R2 = 0,487), el número de enfermedades (R2 = 0,241) y edad (R2 = 0,225). Predominaron los arreglos con los descendientes, siendo los ancianos proveedores y sostén de la familia. Las relaciones encontradas entre configuración familiar, las condiciones de salud física y psicológica fueron importantes. Las mujeres tuvieron mayor carga financiera y peor salud psicológica y física que los hombres.
Descritores: Dextranos/química
Glucanos/química
Oligossacarídeos/análise
-Configuração de Carboidratos
Isomerismo
Espectrometria de Massas por Ionização por Electrospray/métodos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-668286
Autor: Barrientos, Silvia; Rodríguez, Adriana.
Título: Production of glucosyltransferase B and glucans by streptococcus mutans strains isolated from caries-free individuals
Fonte: Acta odontol. latinoam;24(3):258-264, 2011.
Idioma: en.
Resumo: La glucosiltranferasa B es una enzima producida por Streptococcus mutans, que a partir de la sacarosa, cataliza la síntesis de glucanos insolubles los cuales dan soporte a la biopelícula, siendo uno de los principales factores de virulencia para la generación de la caries dental. Sin embargo, no se ha esclarecido su papel en los individuos libre de caries, portadores delmicroorganismo. El objetivo de este estudio fue determinar la producción de glucosiltransferasa B y la producción de glucanos por cepas de Streptococcus mutans aisladas de biopelícula de 30individuos libres de caries. Las cepas fueron cultivadas en caldo Todd Hewitt y las proteínas extracelulares fueron obtenidas por precipitación con sulfato de amonio las proteínas asociadas amembrana por extracción con urea. La presencia de GtfB fue determinada por peso molecular por SDS–PAGE, confirmada por Western Blot utilizando un anticuerpo específico y la producciónde polisacáridos por separación electroforética, incubación con sacarosa y coloración de Schiff. Los resultados muestran que el 96.7 por ciento de las cepas de Streptococcus mutans producen una banda a la altura del peso molecular correspondiente a las Gtf,de las cuales son reactivas por western blot el 63.4 por ciento El 93.3 por cientode las cepas producen polisacáridos. Conclusiones: la cepas de Streptococcus mutans aisladas de biopelícula de individuos sanos producen factores de virulencia asociados a la caries dental como glucosiltransferasa B y glucanos lo que indica que hay condiciones en la cavidad oral diferentes a estos factores que mantienen al individuo libre de caries dental, los cuales deben ser investigados en la búsqueda de estrategias para controlar la enfermedad.
Descritores: Biofilmes
Cárie Dentária/enzimologia
Glucosiltransferases/classificação
Streptococcus mutans/isolamento & purificação
-Western Blotting
Glucanos/fisiologia
Fatores de Virulência
Limites: Seres Humanos
Responsável: AR29.1 - Biblioteca


  6 / 31 LILACS  
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Id: lil-656722
Autor: Yigitler, Cengizhan; Karakas, Dursun Ozgur; Kucukodaci, Zafer; Cosar, Alpaslan; Gülec, Bülent; Akin, Mehmet Levhi.
Título: Adhesion-preventing properties of 4% icodextrin and canola oil: a comparative experimental study
Fonte: Clinics;67(11):1303-1308, Nov. 2012.
Idioma: en.
Resumo: OBJECTIVE: Postsurgical abdominal adhesions are common, serious postoperative complications. The present study compared the usefulness of 4% icodextrin and canola oil in preventing postoperative peritoneal adhesions. METHODS: Twenty-four Wistar albino rats were divided into three groups. Following a laparotomy, a serosal abrasion was made by brushing the cecum, and 3 mL of 0.9% NaCl, 4% icodextrin, or 3 mL of canola oil were intraperitoneally administered for the control, icodextrin, and canola oil groups, respectively. The abdomen was then closed. All of the rats were sacrificed at day 10. Macroscopic, histopathological, and biochemical evaluations were performed. The results were statistically analyzed using Kruskal-Wallis and ANOVA tests. RESULTS: Macroscopic analyses revealed that both canola oil and 4% icodextrin reduced adhesion formation, but the difference was not statistically significant (p = 0.17). The histopathological examinations revealed no significant differences in terms of giant cell, lymphocyte/plasmocyte, neutrophil, ICAM1, or PECAM1 scores. However, both canola oil and 4% icodextrin significantly reduced fibrosis (p = 0.025). In the canola oil group, the histiocytic reactions were significantly increased (p = 0.001), and the hydroxyproline levels were significantly lower than those in the other groups (p = 0.034). CONCLUSIONS: In the present study, canola oil was determined to be superior to 4% icodextrin in lowering hydroxyproline levels and increasing histiocytic reactions. Considering these results, we believe that canola oil is a promising agent for preventing adhesion formation.
Descritores: Ácidos Graxos Monoinsaturados/uso terapêutico
Glucanos/uso terapêutico
Glucose/uso terapêutico
Doenças Peritoneais/prevenção & controle
Peritônio/cirurgia
-Ratos Wistar
Reprodutibilidade dos Testes
Resultado do Tratamento
Aderências Teciduais/prevenção & controle
Limites: Animais
Feminino
Ratos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


  7 / 31 LILACS  
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Id: lil-656679
Autor: Shamala, T. R; Vijayendra, S. V. N; Joshi, G. J.
Título: Agro-industrial residues and starch for growth and co-production of Polyhydroxyalkanoate copolymer and alfa-amylase by Bacillus. SP CFR67îen
Fonte: Braz. j. microbiol;43(3):1094-1102, July-Sept. 2012. graf, tab.
Idioma: en.
Resumo: Polyhydroxyalkanoates (PHA) and α-amylase (α-1,4 glucan-4-glucanohydrolase, E.C. 3.2.1.1) were co-produced by Bacillus sp. CFR-67 using unhydrolysed corn starch as a substrate. Bacterial growth and polymer production were enhanced with the supplementation of hydrolysates of wheat bran (WBH) or rice bran (RBH) individually or in combination (5-20 g L-1, based on weight of soluble substrates-SS). In batch cultivation, a mixture of WBH and RBH (1:1, 10 g L-1 of SS) along with ammonium acetate (1.75 g L-1) and corn starch (30 g L-1) produced maximum quantity of biomass (10 g L-1) and PHA (5.9 g L-1). The polymer thus produced was a copolymer of polyhydroxybutyrate-co-hydroxyvalerate of 95:5 to 90:10 mol%. Presence of WBH and corn starch (10-50 g L-1) in the medium enhanced fermentative yield of α-amylase (2-40 U mL-1 min-1). The enzyme was active in a wide range of pH (4-9) and temperature (40-60ºC). This is the first report on simultaneous production of copolymer of bacterial PHA and α-amylase from unhydrolysed corn starch and agro-industrial residues as substrates.
Descritores: Agroindústria
Bacillus/crescimento & desenvolvimento
Bacillus/isolamento & purificação
Farinha
Glucanos/análise
Hidrolases/análise
Oryza
Poli-Hidroxialcanoatos/análise
Amidos e Féculas
-Ativação Enzimática
Amostras de Alimentos
Microbiologia Industrial
Métodos
Resíduos
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


  8 / 31 LILACS  
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Id: lil-622789
Autor: Gajera, H P; Vakharia, D N.
Título: Production of lytic enzymes by Trichoderma isolates during in vitro antagonism with Aspergillus niger, the causal agent of collar rot of peanut
Fonte: Braz. j. microbiol;43(1):43-52, Jan.-Mar. 2012. ilus, tab.
Idioma: en.
Resumo: Twelve isolates of Trichoderma (six of T. harzianum, five of T. viride, one of T. virens), which reduced variably the incidence of collar rot disease caused in peanut by Aspergillus niger Van Tieghem, were evaluated for their potential to produce lytic enzymes during in vitro antagonism. T. viride 60 inhibited highest (86.2%) growth of test fungus followed by T. harzianum 2J (80.4%) at 6 days after inoculation (DAI) on PDA media. The specific activities of chitinase, â-1,3-glucanase and protease were 11, 3.46 and 9 folds higher in T6 antagonist (T. viride 60 and A. niger interactions) followed by 8.72, 2.85 and 9 folds in T8 antagonist (T. harzianum 2J and A. niger interactions), respectively, compared to the activity produced by control petri plate T13 (A. niger alone) at 6 DAI. Activity of these lytic enzymes induced in antagonists' plates comprises the growth of Trichoderma isolates. However, cellulase and poly galacturonase were found least amount in these antagonists treatment. A significant positive correlation (p=0.01) between percentage growth inhibition of test fungus and lytic enzymes - (chitinase, â-1,3-glucanase and protease) in the culture medium of antagonist treatment established a relationship to inhibit growth of fungal pathogen by increasing the levels of these enzymes. Among the Trichoderma isolates, T. viride 60 was found best strain to be used in biological control of plant pathogen A. niger.
Descritores: Arachis/enzimologia
Aspergillus niger/enzimologia
Aspergillus niger/isolamento & purificação
Glucanos/análise
Técnicas In Vitro
Trichoderma/enzimologia
Trichoderma/isolamento & purificação
-Amostras de Alimentos
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


  9 / 31 LILACS  
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Id: lil-614580
Autor: Kamiya, Regianne Umeko; Taiete, Tiago; Gonçalves, Reginaldo Bruno.
Título: Mutacins of Streptococcus mutans
Fonte: Braz. j. microbiol;42(4):1248-1258, Oct.-Dec. 2011. ilus.
Idioma: en.
Resumo: The colonization and accumulation of Streptococcus mutans are influenced by various factors in the oral cavity, such as nutrition and hygiene conditions of the host, salivary components, cleaning power and salivary flow and characteristics related with microbial virulence factors. Among these virulence factors, the ability to synthesize glucan of adhesion, glucan-binding proteins, lactic acid and bacteriocins could modify the infection process and pathogenesis of this species in the dental biofilm. This review will describe the role of mutacins in transmission, colonization, and/or establishment of S. mutans, the major etiological agent of human dental caries. In addition, we will describe the method for detecting the production of these inhibitory substances in vitro (mutacin typing), classification and diversity of mutacins and the regulatory mechanisms related to its synthesis.
Descritores: Bacteriocinas/análise
Bacteriocinas/isolamento & purificação
Glucanos/análise
Glucanos/isolamento & purificação
Mutação
Placa Dentária/microbiologia
Streptococcus mutans/isolamento & purificação
Streptococcus mutans/patogenicidade
Fatores de Virulência
-Métodos
Pacientes
Técnicas
Virulência
Limites: Seres Humanos
Tipo de Publ: Revisão
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


  10 / 31 LILACS  
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Beltrao, E. I. C
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Id: lil-581487
Autor: Araújo, R. V. S; Melo-Júnior, M. R; Beltrão, E. I. C; Mello, L. A; Iacomini, M; Carneiro-Leão, A. M. A; Carvalho Júnior, L. B; Santos-Magalhães, N. S.
Título: Evaluation of the antischistosomal activity of sulfated α-D-glucan from the lichen Ramalina celastri free and encapsulated into liposomes
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;44(4):311-318, Apr. 2011. ilus, tab.
Idioma: en.
Resumo: The antischistosomal activity of the sulfated polysaccharide α-D-glucan (Glu.SO4) extracted from Ramalina celastri was evaluated after encapsulation into liposomes (Glu.SO4-LIPO) in Schistosoma mansoni-infected mice. The effect of treatment with Glu.SO4 and Glu.SO4-LIPO (10 mg/kg) on egg elimination, worm burden and hepatic granuloma formation was assessed using female albino Swiss mice, 35-40 days of age, weighing 25 ± 2 g, infected with 150 cercariae/animal (Biomphalaria glabrata, BH strain). Four groups (N = 10) were studied, two controls (empty liposomes and NaCl) and two treated groups (Glu.SO4-LIPO and Glu.SO4) using a single dose. Parasitological analysis revealed that Glu.SO4-LIPO was as efficient as Glu.SO4 in reducing egg elimination and worm burden. Treatment with free Glu.SO4 and Glu.SO4-LIPO induced a statistically significant reduction in the number of granulomas (62 and 63 percent, respectively). Lectin histochemistry showed that wheat germ agglutinin intensely stained the egg-granuloma system in all treated groups. On the other hand, peanut agglutinin stained cells in the control groups, but not in the treated groups. The present results suggest a correlation between the decreasing number of hepatic egg-granulomas and the glycosylation profile of the egg-granuloma system in animals treated with free Glu.SO4 or Glu.SO4-LIPO.
Descritores: Anti-Helmínticos/farmacologia
Glucanos/farmacologia
Líquens/química
Extratos Vegetais/farmacologia
Schistosoma mansoni/efeitos dos fármacos
Esquistossomose mansoni/tratamento farmacológico
-Anti-Helmínticos/administração & dosagem
Fezes/parasitologia
Glucanos/administração & dosagem
Glucanos/isolamento & purificação
Imuno-Histoquímica
Intestinos/parasitologia
Intestinos/patologia
Lipossomos
Fígado/parasitologia
Fígado/patologia
Extratos Vegetais/administração & dosagem
Limites: Animais
Feminino
Masculino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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