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Pesquisa : D05.750.078.562.180 [Categoria DeCS]
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  1 / 173 LILACS  
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Id: biblio-949378
Autor: Maia, Guilherme Tavares da Silva; Albuquerque, Amanda Vasconcelos de; Martins Filho, Euclides Dias; Lira Neto, Filipe Tenório de; Souza, Veridiana Sales Barbosa de; Silva, Anderson Arnaldo da; Lira, Mariana Montenegro de Melo; Lima, Salvador Vilar Correia.
Título: Bacterial cellulose to reinforce urethrovesical anastomosis. A translational study
Fonte: Acta cir. bras;33(8):673-683, Aug. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To evaluate the efficacy of the cellulosic exopolysaccharide membrane (CEM) as a urethral reinforcement for urethrovesical anastomosis. Methods: Twenty eight rabbits were submitted to urethrovesical anastomosis with or without CEM reinforcement. The animals were divided into 4 groups: C7, CEM7, C14 and CEM14: (C= only anastomosis or CEM = anastomosis + CEM), evaluated after 7 weeks, and 14 weeks. The biointegration and biocompatibility of CEM were evaluated according to stenosis, fistula, urethral wall thickness, urethral epithelium, rate of inflammation and vascularization. Results: Between the two experimental groups, the difference in the number of stenosis or urinary fistula was not statistically significant. The morphometric analysis revealed preservation of urethral lumen, well adhered CEM without extrusion, a controlled inflammatory process and implant vascularization. The urothelium height remained constant over time after CEM reinforcement and the membrane wall was thicker, statistically, after 14 weeks. Conclusion: The absence of extrusion, stenosis or urinary fistula after 14 weeks of urethrovesical anastomosis demonstrates cellulosic exopolysaccharide membrane biocompatibility and biointegration with tendency to a thicker wall.
Descritores: Uretra/cirurgia
Materiais Biocompatíveis/uso terapêutico
Bexiga Urinária/cirurgia
Celulose/uso terapêutico
-Polissacarídeos Bacterianos/uso terapêutico
Fatores de Tempo
Uretra/patologia
Bexiga Urinária/patologia
Microbiologia Industrial/métodos
Teste de Materiais
Anastomose Cirúrgica
Celulose/biossíntese
Reprodutibilidade dos Testes
Resultado do Tratamento
Pesquisa Médica Translacional
Neovascularização Patológica
Limites: Animais
Masculino
Coelhos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  2 / 173 LILACS  
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Id: biblio-974324
Autor: Revin, Victor; Liyaskina, Elena; Nazarkina, Maria; Bogatyreva, Alena; Shchankin, Mikhail.
Título: Cost-effective production of bacterial cellulose using acidic food industry by-products
Fonte: Braz. j. microbiol;49(supl.1):151-159, 2018. tab, graf.
Idioma: en.
Resumo: Abstract To reduce the cost of obtaining bacterial cellulose, acidic by-products of the alcohol and dairy industries were used without any pretreatment or addition of other nitrogen sources. Studies have shown that the greatest accumulation of bacterial cellulose (6.19 g/L) occurs on wheat thin stillage for 3 days of cultivation under dynamic conditions, which is almost 3 times higher than on standard Hestrin and Schramm medium (2.14 g/L). The use of whey as a nutrient medium makes it possible to obtain 5.45 g/L bacterial cellulose under similar conditions of cultivation. It is established that the pH of the medium during the growth of Gluconacetobacter sucrofermentans B-11267 depends on the feedstock used and its initial value. By culturing the bacterium on thin stillage and whey, there is a decrease in the acidity of the waste. It is shown that the infrared spectra of bacterial cellulose obtained in a variety of environments have a similar character, but we found differences in the micromorphology and crystallinity of the resulting biopolymer.
Descritores: Resíduos/análise
Microbiologia Industrial/métodos
Celulose/biossíntese
Gluconacetobacter/metabolismo
-Resíduos/economia
Triticum/metabolismo
Triticum/microbiologia
Microbiologia Industrial/economia
Indústria Alimentícia
Meios de Cultura/economia
Meios de Cultura/metabolismo
Gluconacetobacter/crescimento & desenvolvimento
Etanol/metabolismo
Responsável: BR1.1 - BIREME


  3 / 173 LILACS  
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Id: biblio-828185
Autor: Castillo-Castillo, Y; Ruiz-Barrera, O; Burrola-Barraza, M. E; Marrero-Rodriguez, Y; Salinas-Chavira, J; Angulo-Montoya, C; Corral-Luna, A; Arzola-Alvarez, C; Itza-Ortiz, M; Camarillo, J.
Título: Isolation and characterization of yeasts from fermented apple bagasse as additives for ruminant feeding
Fonte: Braz. j. microbiol;47(4):889-895, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract Solid-state fermentation can be used to produce feeds for ruminants, which can provide an enriched population of yeasts to improve ruminal fermentation. Fermentation of apple bagasse was performed to obtain a yeast-rich product, with the objective of isolating, identifying, and characterizing yeast strains and testing their capability to enhance in vitro ruminal fermentation of fibrous feeds. Yeasts were isolated from apple bagasse fermented under in vitro conditions, using rumen liquor obtained from cannulated cows and alfalfa as a fibrous substrate. A total of 16 new yeast strains were isolated and identified by biochemical and molecular methods. The strains were designated Levazot, followed by the isolate number. Their fermentative capacity was assessed using an in vitro gas production method. Strain Levazot 15 (Candida norvegensis) showed the greatest increase in gas production (p < 0.05) compared with the yeast-free control and positively affected in vitro ruminal fermentation parameters of alfalfa and oat straw. Based on these results, it was concluded that the Levazot 15 yeast strain could be potentially used as an additive for ruminants consuming high-fiber diets. However, further studies of effects of these additives on rumen digestion, metabolism, and productive performance of ruminants are required.
Descritores: Leveduras/isolamento & purificação
Leveduras/classificação
Celulose
Malus
Aditivos Alimentares
Ração Animal/microbiologia
-Filogenia
Leveduras/genética
Leveduras/metabolismo
Ruminantes
Fermentação
Limites: Animais
Responsável: BR1.1 - BIREME


  4 / 173 LILACS  
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Id: lil-780841
Autor: Bottino, Flávia; Cunha-Santino, Marcela Bianchessi; Bianchini Jr, Irineu.
Título: Cellulase activity and dissolved organic carbon release from lignocellulose macrophyte-derived in four trophic conditions
Fonte: Braz. j. microbiol;47(2):352-358, Apr.-June 2016. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation.
Resumo: Abstract Considering the importance of lignocellulose macrophyte-derived for the energy flux in aquatic ecosystems and the nutrient concentrations as a function of force which influences the decomposition process, this study aims to relate the enzymatic activity and lignocellulose hydrolysis in different trophic statuses. Water samples and two macrophyte species were collected from the littoral zone of a subtropical Brazilian Reservoir. A lignocellulosic matrix was obtained using aqueous extraction of dried plant material (≈40 °C). Incubations for decomposition of the lignocellulosic matrix were prepared using lignocelluloses, inoculums and filtered water simulating different trophic statuses with the same N:P ratio. The particulate organic carbon and dissolved organic carbon (POC and DOC, respectively) were quantified, the cellulase enzymatic activity was measured by releasing reducing sugars and immobilized carbon was analyzed by filtration. During the cellulose degradation indicated by the cellulase activity, the dissolved organic carbon daily rate and enzyme activity increased. It was related to a fast hydrolysable fraction of cellulose that contributed to short-term carbon immobilization (ca. 10 days). After approximately 20 days, the dissolved organic carbon and enzyme activity were inversely correlated suggesting that the respiration of microorganisms was responsible for carbon mineralization. Cellulose was an important resource in low nutrient conditions (oligotrophic). However, the detritus quality played a major role in the lignocelluloses degradation (i.e., enzyme activity) and carbon release.
Descritores: Bactérias/enzimologia
Proteínas de Bactérias/metabolismo
Celulase/metabolismo
Araceae/metabolismo
Paspalum/metabolismo
Água Doce/química
Lignina/metabolismo
-Brasil
Carbono/metabolismo
Celulose/genética
Celulose/metabolismo
Ecossistema
Araceae/crescimento & desenvolvimento
Araceae/microbiologia
Paspalum/crescimento & desenvolvimento
Paspalum/microbiologia
Água Doce/microbiologia
Responsável: BR1.1 - BIREME


  5 / 173 LILACS  
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Id: biblio-1022118
Autor: Silva Lemões, Juliana; Lemons e Silva, Claudia Fernanda; Farias Avila, Sabrina Peres; Scherrer Montero, Cândida Raquel; Anjos e Silva, Sérgio Delmar dos; Samios, Dimitrios; Ruaro Peralba, Maria do Carmo.
Título: Chemical pretreatment of Arundo donax L. for second-generation ethanol production
Fonte: Electron. j. biotechnol;31:67-54, Jan. 2018. ilus, tab, graf.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: Background: Pretreatment of lignocellulosic biomass is essential for using it as a raw material for chemical and biofuel production. This study evaluates the effects of variables in the chemical pretreatment of the Arundo biomass on the glucose and xylose concentrations in the final enzymatic hydrolysate. Three pretreatments were tested: acid pretreatment, acid pretreatment followed by alkaline pretreatment, and alkaline pretreatment. Results: The amounts of glucose and xylose released by the enzymatic hydrolysis of the Arundo biomass obtained from acid pretreatment ranged from 6.2 to 19.1 g/L and 1.8 to 3.1 g/L, respectively. The addition of alkaline pretreatment led to a higher yield from the enzymatic hydrolysis, with the average glucose concentration 3.5 times that obtained after biomass hydrolysis with an acid pretreatment exclusively. The use of an alkaline pretreatment alone resulted in glucose and xylose concentrations similar to those obtained in the two-step pretreatment: acid pretreatment followed by alkaline pretreatment. There was no significant difference in 5-hydroxymethylfurfural, furfural, or acetic acid concentrations among the pretreatments. Conclusion: Alkaline pretreatment was essential for obtaining high concentrations of glucose and xylose. The application of an alkaline pretreatment alone resulted in high glucose and xylose concentrations. This result is very significant as it allows a cost reduction by eliminating one step.
Descritores: Etanol/metabolismo
Poaceae/química
-Ácidos/química
Xilose/análise
Celulose/química
Biomassa
Biocombustíveis
Glucose/análise
Hidrólise
Lignina
Responsável: CL1.1 - Biblioteca Central


  6 / 173 LILACS  
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Id: biblio-1010145
Autor: Lin, Qian; Li, Dongni; Qin, Huizhen.
Título: Molecular cloning, expression, and immobilization of glutamate decarboxylase from Lactobacillus fermentum YS2
Fonte: Electron. j. biotechnol;27:8-13, May. 2017. ilus, graf.
Idioma: en.
Projeto: Guangxi Colleges and Universities Scientific Research.
Resumo: Background: GABA (γ-aminobutyric acid) is a four-carbon nonprotein amino acid that has hypotensive, diuretic, and tranquilizing properties. Glutamate decarboxylase (GAD) is the key enzyme to generate GABA. A simple and economical method of preparing and immobilizing GAD would be helpful for GABA production. In this study, the GAD from Lactobacillus fermentum YS2 was expressed under the control of a stress-inducible promoter and was purified and immobilized in a fusion form, and its reusability was investigated. Results: The fusion protein CBM-GAD was expressed in Escherichia coli DH5α carrying pCROCB-gadB, which contained promoter PrpoS, cbm3 (family 3 carbohydrate-binding module from Clostridium thermocellum) coding sequence, the gadB gene from L. fermentum YS2 coding for GAD, and the T7 terminator. After a one-step purification of CBM-GAD using regenerated amorphous cellulose (RAC) as an adsorbent, SDS-PAGE analysis revealed a clear band of 71 kDa; the specific activity of the purified fusion protein CBM-GAD reached 83.6 ± 0.7 U·mg-1. After adsorption onto RAC, the immobilized GAD with CBM3 tag was repeatedly used for GABA synthesis. The protein-binding capacity of RAC was 174 ± 8 mg·g-1. The immobilized CBM-GAD could repeatedly catalyze GABA synthesis, and 8% of the initial activities was retained after 10 uses. We tested the conversion of monosodium glutamate to GABA by the immobilized enzyme; the yield reached 5.15 g/L and the productivity reached 3.09 g/L·h. Conclusions: RAC could be used as an adsorbent in one-step purification and immobilization of CBM-GAD, and the immobilized enzyme could be repeatedly used to catalyze the conversion of glutamate to GABA.
Descritores: Lactobacillus fermentum/enzimologia
Glutamato Descarboxilase/genética
Glutamato Descarboxilase/metabolismo
-Temperatura
Proteínas Recombinantes de Fusão
Celulose
Clonagem Molecular
Adsorção
Enzimas Imobilizadas
Escherichia coli
Ácido gama-Aminobutírico/biossíntese
Concentração de Íons de Hidrogênio
Responsável: CL1.1 - Biblioteca Central


  7 / 173 LILACS  
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Id: biblio-839144
Autor: Menezes-Silva, Rafael; Pereira, Fabiano Vargas; Santos, Maria Helena; Soares, Janir Alves; Soares, Suelleng Maria Cunha Santos; Miranda, João Luiz de.
Título: Biocompatibility of a New Dental Glass Ionomer Cement with Cellulose Microfibers and Cellulose Nanocrystals
Fonte: Braz. dent. j;28(2):172-178, mar.-Apr. 2017. tab, graf.
Idioma: pt.
Projeto: SECTES/FAPEMIG.
Resumo: Resumo Os novos materiais restauradores em desenvolvimento devem evitar danos aos tecidos dentários. Portanto, o objetivo deste estudo foi avaliar a biocompatibilidade de uma marca comercial de cimento de ionômero de vidro convencional (CIV) modificado com microfibras de celulose (CIV+MC) ou nanocristais de celulose (CIV+NC) através da implantação de três amostras em tecido subcutâneo na região dorsal de 15 ratos Rattus norvegicus albinus. Cada rato recebeu um exemplar de cada cimento, resultando nos seguintes grupos (n=15): Grupo CIV (controle, n=15), Grupo CIV+MC e Grupo CIV+NC. Nos intervalos de 7, 30 e 60 dias os animais foram sacrificados e os seguintes aspectos foram avaliados histologicamente: tipo de células inflamatórias, fibroblastos, vasos sanguíneos, macrófagos, células gigantes, tipo de reação inflamatória e espessura da cápsula (µm). Estes eventos foram quantitativamente classificados conforme os escores: (-) ausente, (+) suave, (++) moderado e (+++) intenso. Os resultados foram analisados estatisticamente pelo teste Kruskal-Wallis e pós-teste Mann-Whitney. Aos 7 dias, o Grupo CIV+NC demonstrou um nível mais elevado de reparação tecidual porque havia maior quantidade de fibroblastos (p=0,022) e uma menor quantidade de macrófagos (p=0,008) e células mononucleares (p=0,033). Neutrófilos e células gigantes estavam ausentes em todos os períodos experimentais. Aos 60 dias, o Grupo CIV+NC apresentou cápsula de tecido fibroso com espessura mais reduzida (26,72±2,87 µm) em comparação ao Grupo CIV+MC (41,21±3,98 µm (p=0,025). No geral, todos os materiais apresentaram satisfatória biocompatibilidade, no entanto, o cimento de ionômero de vidro modificado com nanocristais de celulose proveu reparação tecidual mais avançada comparativamente aos demais materiais avaliados.

Developing new restorative materials should avoid damage to tissue structures. This study evaluated the biocompatibility of a commercial dental glass ionomer cement (GIC) mechanically reinforced with cellulose microfibers (GIC+CM) or cellulose nanocrystals (GIC+CN) by implantation of three test specimens in subcutaneous tissue in the dorsal region of 15 Rattus norvegicus albinus rats. Each rat received one specimen of each cement, resulting in the following groups (n=15): Group GIC (Control), Group GIC+CM and Group GIC+NC. After time intervals of 7, 30 and 60 days, the animals were sacrificed and the following aspects were histologically evaluated: type of inflammatory cells, fibroblasts, blood vessels, macrophages, giant cells, type of inflammatory reaction and capsule thickness (µm). These events were scored as (-) absent, (+) light, (++) moderate and (+++) intense. The results were statistically analyzed by Kruskal-Wallis test and Mann-Whitney post test. At 7 days, Group GIC+NC showed more favorable tissue repair because quantitatively there were more fibroblasts (p=0.022), fewer macrophages (p=0.008) and mononuclear cells (p=0.033). Polymorphonuclear neutrophils and giant cells were absent in all experimental periods. At 60 days, test specimens in Group GIC+NC were surrounded by a fibrous tissue capsule with reduced thickness (26.72±2.87 µm) in comparison with Group GIC+CM (41.21±3.98 µm) (p=0.025). In general, all biomaterials showed satisfactory biocompatibility, but glass ionomer cement modified with cellulose nanocrystals showed a more advanced tissue repair.
Descritores: Materiais Biocompatíveis
Celulose/química
Nanopartículas/química
Cimentos de Ionômeros de Vidro/química
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


  8 / 173 LILACS  
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Id: biblio-1010399
Autor: Seneesrisakul, Kessara; Albayrak Guralp, Saadet; Gulari, Erdogan; Chavadej, Sumaeth.
Título: Escherichia coli expressing endoglucanase gene from Thai higher termite bacteria for enzymatic and microbial hydrolysis of cellulosic materials
Fonte: Electron. j. biotechnol;27:70-79, May. 2017. tab, ilus, graf.
Idioma: en.
Resumo: Background: Endoglucanase plays a major role in initiating cellulose hydrolysis. Various wild-type strains were searched to produce this enzyme, but mostly low extracellular enzyme activities were obtained. To improve extracellular enzyme production for potential industrial applications, the endoglucanase gene of Bacillus subtilis M015, isolated from Thai higher termite, was expressed in a periplasmic-leaky Escherichia coli. Then, the crude recombinant endoglucanase (EglS) along with a commercial cellulase (Cel) was used for hydrolyzing celluloses and microbial hydrolysis using whole bacterial cells. Results: E. coli Glu5 expressing endoglucanase at high levels was successfully constructed. It produced EglS (55 kDa) with extracellular activity of 18.56 U/mg total protein at optimal hydrolytic conditions (pH 4.8 and 50°C). EglS was highly stable (over 80% activity retained) at 40­50°C after 100 h. The addition of EglS significantly improved the initial sugar production rates of Cel on the hydrolysis of carboxymethyl cellulose (CMC), microcrystalline cellulose, and corncob about 5.2-, 1.7-, and 4.0-folds, respectively, compared to those with Cel alone. E. coli Glu5 could secrete EglS with high activity in the presence of glucose (1% w/v) and Tween 80 (5% w/v) with low glucose consumption. Microbial hydrolysis of CMC using E. coli Glu5 yielded 26 mg reducing sugar/g CMC at pH 7.0 and 37°C after 48 h. Conclusions: The recombinant endoglucanase activity improved by 17 times compared with that of the native strain and could greatly enhance the enzymatic hydrolysis of all studied celluloses when combined with a commercial cellulase.
Descritores: Bacillus subtilis/enzimologia
Celulase/metabolismo
Isópteros/microbiologia
-Tailândia
Proteínas Recombinantes/metabolismo
Celulase/genética
Celulose
Amplificação de Genes
Agricultura
Escherichia coli/metabolismo
Hidrólise
Responsável: CL1.1 - Biblioteca Central


  9 / 173 LILACS  
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Id: biblio-1046371
Autor: Chernigova, S. V; Zubkova, N. V; Chernigov, Yu V; Pogorelova, N. A.
Título: Morphological changes in the tissue structures afeter thermal burns on the background of using dermarm wound dressing
Fonte: Prensa méd. argent;105(9 especial):521-525, oct 2019. tab.
Idioma: en.
Resumo: Over the last decade, the interest in using bacterial cellulose in medicine has increased. The article publishes the data about the efficiency of healing burn wounds in rabbits in experimental conditions with the use of the DermaRM wound dressing, compared to the traditionally used Panthenol ointment and the Branolind N salve dressing
Descritores: Pomadas/uso terapêutico
Bandagens
Queimaduras/terapia
Celulose/uso terapêutico
Resultado do Tratamento
Tempo para o Tratamento
Limites: Animais
Coelhos
Responsável: AR392.1 - Biblioteca


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Id: biblio-966935
Autor: Tanskul, Somporn; Damthongsen, Tassanaporn; Jaturonlak, Nathakan.
Título: A medium supplemented with vegetable extract used for cellulose production of Rhodococcus SP. MI 2 / Meio suplementado com extrato vegetal utilizado na produção de celulose de Rhodococcus SP. MI 2
Fonte: Biosci. j. (Online);34(3):666-673, mai/jun. 2018. graf, tab, ilus.
Idioma: en.
Resumo: In this study, the most suitable vegetable extract was screened to use as non-conventional nutrient sources for cellulose production of Rhodococcus sp. MI 2. SH medium or a synthetic medium was used as a conventional or control medium. Cha-poo (Piper sarmentosum Roxb.) and sweet potato (Ipomoea batatas Lam.) were 2 out of 14 vegetable extracts chosen as medium supplements. Rhodococcus sp. MI 2 gave the highest cellulose yield in a medium supplemented with Cha-poo extract. The optimum culture conditions in the medium supplemented with Cha-poo extract at room temperature (25o C) under static condition were 5% (v v-1) inoculum size, a 6 -day -incubation period, pH 3, 3% sucrose, and 0.5% (NH4)2SO4. The cellulose yield in the medium supplemented with Cha-poo extract was increased about 3 times (6.83 g L-1 during 6 days) higher than that obtained before optimizing (2.39 g L-1 during 6 days). The medium supplemented with Cha-poo extract cost one quarter (0.5 USD L-1 of medium) of the SH medium (1.9 USD L-1 of medium). The structure of the microfibrils of cellulose produced by Rhodococcus sp. MI 2 in a medium supplemented with Cha-poo extract observed by SEM had larger, less crowded fibrils than those produced in the medium supplemented with sweet potato extract. In addition, the microfibrils of the former had many beehive shaped knots whereas those of the latter had mantle-like surrounding the fibrils.

Neste estudo, o extrato vegetal mais adequado foi triado para uso como fontes não convencionais de nutrientes para produção de celulose de Rhodococcus sp. MI 2. Utilizou-se um meio SH ou meio sintético como meio convencional ou de controle. Cha-poo (Piper sarmentosum Roxb.) e batata-doce (Ipomoea batatas Lam.) foram 2 dos 14 extratos vegetais escolhidos como suplementos do meio. Rhodococcus sp. MI 2 deu o maior rendimento de celulose em um meio suplementado com extrato de Cha-poo. As condições ótimas de cultivo no meio suplementado com extrato de Cha-poo em temperatura ambiente (25 ºC) em condição estática foram 5% (v v-1) do tamanho do inóculo, um período de 6 dias de incubação, pH 3, 3% de sacarose, e 0,5% (NH4) 2SO4. O rendimento de celulose no meio suplementado com extrato de Cha-poo foi aumentado cerca de 3 vezes (6,83 g L-1 durante 6 dias), maior do que o obtido antes da otimização (2,39 g L-1 durante 6 dias). O meio suplementado com extrato de Cha-poo custou um quarto (0,5 USD L-1 de meio) do meio SH (1,9 USD L-1 de meio). A estrutura das microfibrilas de celulose produzidas por Rhodococcus sp. MI 2 em meio suplementado com extrato de Cha-poo, observado por MEV, apresentou fibrilas maiores e menos congestionadas do que aquelas produzidas no meio suplementado com extrato de batata-doce. Além disso, as microfibrilas do primeiro possuíam muitos nós em forma de colmeia, enquanto os do último tinham um aspecto tipo manto ao redor das fibras.
Descritores: Bactérias
Extratos Vegetais
Celulose
Ipomoea batatas
-Rhodococcus
Responsável: BR396.1 - Biblioteca Central



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