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Id: lil-572126
Autor: Rodríguez, Héctor; Silva, Iván; Needham, Douglas; Ossandón, Enrique; Cuevas, Miguel Angel; Sánchez, Carlos; Castro, María Eugenia; Sarabia, Luis; Arias, Mauricio; Espinoza, Omar; González, Raúl; Araya, Juan Carlos.
Título: Inmunohistoquímica de la inervación del conducto deferente humano / Immunohistochemistry in the innervations of the human vas deferens
Fonte: Rev. chil. tecnol. méd;30(1):1545-1550, 2010. ilus.
Idioma: es.
Projeto: FONDECYT.
Resumo: El conducto deferente humano presenta una pared muscular gruesa, donde el componente muscular liso ocupa la parte media y más prominente. Esta composición histológica le permite al órgano desarrollar las potentes contracciones durante el proceso de la eyaculación y emisión del semen. Objetivos: Analizar la presencia y distribución de la positividad inmunohistoquímica a Neurofilamentos (NF) en las paredes del conducto deferente humano. Pacientes, Materiales y Método: De tres pacientes sometidos a orquiectomía radical por diagnóstico de Seminoma, se obtuvieron los conductos deferentes fijados en formol tamponado (pH 7,2). Mediante procedimientos histológicos de rutina, se obtuvieron secciones de 5 um de espesor en portaobjetos silanizados. Se procedió al desarrollo del protocolo de inmunohistoquímica usando anticuerpos específicos contra Neurofilamentos (NF); las imágenes se obtuvieron con cámara fotográfica digital CCD Micrometrics, en microscopio óptico Olympus CX31. Resultados: En los subcompartimientos de las secciones transversales de la pared de los conductos deferentes humanos, se observa la reacción inmunohistoquímica positiva a NF. Sin embargo, los fascículos nerviosos se concentran en la adventicia, mientras que en la mucosa y pared muscular son en extremo escasos y finos. Conclusión: En el conducto deferente existe una inervación preferencial dispuesta en la adventicia del órgano, siendo posible que la potencia de la contracción de la pared en base a la actividad muscular, requiera factores adicionales de estimulación.

The human vas deferens has a thick muscular wall, where the smooth muscle component occupies the middle and most prominent. This composition allows the organ histologic develop powerful contractions during ejaculation process and the semen. Objectives. To analyze the presence and distribution of immunologically positlve for Neurofilament (NF) on the walls of human vas deferens. Patients, Materiall and Methods.' Three patients undergoing radical orchiectomy for seminoma diagnosis were obtained vas deferens fixed in buffered formain (pH 12). By routine histological procedures, sections were obtained 5 um thick on silylated slides. We proceeded to the development of immunohistochemical protocol using specific antibodies against Neurofilament (NF); the digitized images were obtained with CCO Micrometrics digital camera, in the light microscope Olympus CX31. Results: In the subcompartments of the cross sections of the wall of human vas deferens, there is a positive immunohistochemical reaction to NF However, nerve bundles are concentrated in the adventitia, whereas in the mucosa and muscle wall are extremely rare and fine. Conclusion: In the vas deferens there willing preferential innervation in the adventitia of the court, it being possible for the power of contraction of the wall of the body based on muscle activity, stimulation requires additional factors.
Descritores: Ducto Deferente/inervação
-Ducto Deferente/imunologia
Ducto Deferente/ultraestrutura
Imuno-Histoquímica
Proteínas de Neurofilamentos/imunologia
Limites: Seres Humanos
Adulto
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
Texto completo
Id: lil-433163
Autor: Antunes, S. L. G; Chimelli, L. M; Rabello, E. T; Valentim, V. C; Corte-Real, S; Sarno, E. N; Jardim, M. R.
Título: An immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosy
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;39(8):1071-1081, Aug. 2006. ilus, tab.
Idioma: en.
Resumo: The nerve biopsies of 11 patients with pure neuritic leprosy were submitted to routine diagnostic procedures and immunoperoxidase staining with antibodies against axonal (neurofilament, nerve growth factor receptor (NGFr), and protein gene product (PGP) 9.5) and Schwann cell (myelin basic protein, S-100 protein, and NGFr) markers. Two pairs of non-adjacent histological cross-sections of the peripheral nerve were removed for quantification. All the fascicles of the nerve were examined with a 10X-ocular and 40X-objective lens. The immunohistochemistry results were compared to the results of semithin section analysis and clinical and electroneuromyographic data. Neurofilament staining was reduced in 100 percent of the neuritic biopsies. NGFr positivity was also reduced in 81.8 percent, PGP staining in 100 percent of the affected nerves, S100 positivity in 90.9 percent, and myelin basic protein immunoreactivity in 90.9 percent. Hypoesthesia was associated with decreased NGFr (81.8 percent) and PGP staining (90.9 percent). Reduced potential amplitudes (electroneuromyographic data) were found to be associated with reduced PGP 9.5 (63.6 percent) and nerve fiber neurofilament staining (45.4 percent) by immunohistochemistry and with loss of myelinated fibers (100 percent) by semithin section analysis. On the other hand, the small fibers (immunoreactive dots) seen amid inflammatory cells continued to be present even after 40 percent of the larger myelinated fibers had disappeared. The present study shows an in-depth view of the destructive effects of leprosy upon the expression of neural markers and the integrity of nerve fiber. The association of these structural changes with the clinical and electroneuromyographic manifestations of leprosy peripheral neuropathy was also discussed.
Descritores: Antígenos de Bactérias/análise
Glicolipídeos/análise
Hanseníase/diagnóstico
Mycobacterium leprae/imunologia
Fibras Nervosas Mielinizadas/patologia
Proteínas do Tecido Nervoso/análise
Neurite (Inflamação)/diagnóstico
-Antígenos de Bactérias/imunologia
Biópsia
Biomarcadores/análise
DNA Bacteriano/análise
Eletromiografia
Glicolipídeos/imunologia
Técnicas Imunoenzimáticas
Imuno-Histoquímica
Hanseníase/patologia
Proteína Básica da Mielina
Mycobacterium leprae/genética
Neurite (Inflamação)/patologia
Proteínas de Neurofilamentos/análise
Reação em Cadeia da Polimerase
Receptores de Fator de Crescimento Neural/análise
/análise
SACETOLACTATE SYNTHASE PROTEINS/análise
Limites: Adulto
Feminino
Seres Humanos
Masculino
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
Texto completo
Id: lil-402671
Autor: Mendonça, D. M. F; Chimelli, L; Martinez, A. M. B.
Título: Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;38(6):925-933, June 2005. ilus, tab.
Idioma: en.
Resumo: Amyotrophic lateral sclerosis (ALS), a neurodegenerative disease of unknown etiology, affects motor neurons leading to atrophy of skeletal muscles, paralysis and death. There is evidence for the accumulation of neurofilaments (NF) in motor neurons of the spinal cord in ALS cases. NF are major structural elements of the neuronal cytoskeleton. They play an important role in cell architecture and differentiation and in the determination and maintenance of fiber caliber. They are composed of three different polypeptides: light (NF-L), medium (NF-M) and heavy (NF-H) subunits. In the present study, we performed a morphological and quantitative immunohistochemical analysis to evaluate the accumulation of NF and the presence of each subunit in control and ALS cases. Spinal cords from patients without neurological disease and from ALS patients were obtained at autopsy. In all ALS cases there was a marked loss of motor neurons, besides atrophic neurons and preserved neurons with cytoplasmic inclusions, and extensive gliosis. In control cases, the immunoreaction in the cytoplasm of neurons was weak for phosphorylated NF-H, strong for NF-M and weak for NF-L. In ALS cases, anterior horn neurons showed intense immunoreactivity in focal regions of neuronal perikarya for all subunits, although the difference in the integrated optical density was statistically significant only for NF-H. Furthermore, we also observed dilated axons (spheroids), which were immunopositive for NF-H but negative for NF-M and NF-L. In conclusion, we present qualitative and quantitative evidence of NF-H subunit accumulation in neuronal perikarya and spheroids, which suggests a possible role of this subunit in the pathogenesis of ALS.
Descritores: Esclerose Amiotrófica Lateral/metabolismo
Neurônios Motores/química
Proteínas de Neurofilamentos/análise
Medula Espinal/patologia
-Esclerose Amiotrófica Lateral/patologia
Biomarcadores/análise
Estudos de Casos e Controles
Imuno-Histoquímica
Neurônios Motores/patologia
Limites: Adulto
Idoso
Seres Humanos
Masculino
Meia-Idade
Responsável: BR1.1 - BIREME


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Id: lil-336005
Autor: Capani, F; Loidl, C. F; Saavedra, J. Pecci.
Título: Unilateral enucleation induces an increase of 160 kd neurofilament in lateral geniculate nuclei synapses
Fonte: Biocell;20(1):55-59, Apr. 1996.
Idioma: en.
Resumo: Ultrastructural synaptic changes of retinal origin in the pars dorsalis lateral geniculate nuclei (dLGN) after enucleation have been studied in this laboratory, showing a filamentous hypertrophy with maximal expression at 4-6 days post-lesion in monkeys (Pecci Saavedra et al., 1970, 1971). The aim of this work was to elucidate the nature of the newly formed filament in dLGN in post-enucleated rats. Male Wistar rats were fixed with 4 paraformaldehyde plus 0.25 glutaraldehyde in 0.1M phosphate buffer, through the abdominal aorta after 3, 5, and 7 days postenucleation. Sections obtained were incubated with antibodies to the phosphorylated portion of the 160 Kd neurofilaments (1:3000) and anti-GFAP (1:25000). There was an increase in 160 Kd neurofilament staining in axons and degenerating nerve endings in dLGN, as well as a typical astroglial immunostained reaction. Our results show that the newly formed neurofilaments after deafferentation are of the 160 Kd type, commonly present in normal axons.
Descritores: Enucleação Ocular
Corpos Geniculados
Proteínas de Neurofilamentos/metabolismo
Sinapses
-Astrócitos
Degeneração Neural/fisiopatologia
Corpos Geniculados
Neurônios/química
Neurônios/metabolismo
Neurônios/ultraestrutura
Proteína Glial Fibrilar Ácida/análise
Proteínas de Neurofilamentos/análise
Ratos Wistar
Sinapses
Limites: Animais
Masculino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
Chadi, Gerson
Texto completo
Id: lil-209225
Autor: Chadi, Gerson; Castelucci, Patrícia; Gomide, Vânia C.
Título: Experimental microneurosurgery of the central and peripheral nervous system in the study of the neuronal and glial trophism and plasticity
Fonte: Acta cir. bras;13(1):8-17, jan.-mar. 1998. ilus, tab, graf.
Idioma: en.
Projeto: FAPESP; . CNPq; . Sandoz Foundation for Gerontological Research; . TWAS.
Resumo: Trauma and neurodegenerative diseases commit the nervous system. After an axotomy or nerve injury in the peripheral nervous system the regeneration of the nerve fibers and reinervation of the target are seen. In central nervous system these events are restrictive, however their occurrence are related to the state of glial reaction and the synthesis of neurotrophic factors. Basic fibroblast growth factor (bFGF) has been considered an important trophic factor for neurons and astrocytes of many central nervous system regions. In this study rats were submitted to one of following neurosurgery procedures: callosotomy, pyramidectomy or complete transection of hypoglossal nerve (XII). Sham operations were made in control animals. Seven days later animals were sacrificed and their braims processed for immunohistochemistry. Coronal sections were taken from the central nervous system and incubated with antisera against the glial fibrillary acidic protein (GFAP) or neurofilament (NF), markers for astrocyte and neuronal cell body and fibers, respectively, as well as with the antiserum against the bFGF. The degree of the labelling was quatified with computer assisted stereological methods. The analysis of the NF immunoreactivity revealed a disappearance of fibers in the white matter distal to the pyramidectomy and callosotomy, however no disapperance of NF immunoreactive neurons was found in the XII nucleus following axotomy. These changes was accompanied by a massive astrocytic reaction. The reactive astrocytes synthesized increased amounts of bFGF. These findings suggest that glial reaction synthesizing neurotrophic factors may influence the wound and repair after mechanical lesions of central nervous and subsequent neuronal trophism and plasticity which may be relevant to the regenerative process of the nervous tissue.
Descritores: Sistema Nervoso Central/cirurgia
Microcirurgia
Neuroglia/fisiologia
Plasticidade Neuronal
Neurônios/fisiologia
Procedimentos Neurocirúrgicos
Proteína Glial Fibrilar Ácida/fisiologia
Proteínas de Neurofilamentos/fisiologia
Sistema Nervoso Periférico/cirurgia
Tropismo
-Astrócitos/fisiologia
Fator 2 de Crescimento de Fibroblastos
Regeneração Nervosa
Ratos Wistar
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME



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