Base de dados : LILACS
Pesquisa : D05.750.078.730.475.750 [Categoria DeCS]
Referências encontradas : 7 [refinar]
Mostrando: 1 .. 7   no formato [Detalhado]

página 1 de 1

  1 / 7 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-668777
Autor: Brazilian Journal of Medical and Biological Research; Souza, C.C.R.; Dombroski, T.C.D.; Machado, H.R.; Oliveira, R.S.; Rocha, L.B.; Rodrigues, A.R.A.; Neder, L.; Chimelli, L.; Corrêa, V.M.A.; Larson, R.E.; Martins, A.R..
Título: Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old age
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;46(2):164-170, 01/fev. 2013. tab, graf.
Idioma: en.
Resumo: Myosin Va functions as a processive, actin-based motor molecule highly enriched in the nervous system, which transports and/or tethers organelles, vesicles, and mRNA and protein translation machinery. Mutation of myosin Va leads to Griscelli disease that is associated with severe neurological deficits and a short life span. Despite playing a critical role in development, the expression of myosin Va in the central nervous system throughout the human life span has not been reported. To address this issue, the cerebellar expression of myosin Va from newborns to elderly humans was studied by immunohistochemistry using an affinity-purified anti-myosin Va antibody. Myosin Va was expressed at all ages from the 10th postnatal day to the 98th year of life, in molecular, Purkinje and granular cerebellar layers. Cerebellar myosin Va expression did not differ essentially in localization or intensity from childhood to old age, except during the postnatal developmental period. Structures resembling granules and climbing fibers in Purkinje cells were deeply stained. In dentate neurons, long processes were deeply stained by anti-myosin Va, as were punctate nuclear structures. During the first postnatal year, myosin Va was differentially expressed in the external granular layer (EGL). In the EGL, proliferating prospective granule cells were not stained by anti-myosin Va antibody. In contrast, premigratory granule cells in the EGL stained moderately. Granule cells exhibiting a migratory profile in the molecular layer were also moderately stained. In conclusion, neuronal myosin Va is developmentally regulated, and appears to be required for cerebellar function from early postnatal life to senescence.
Descritores: Cerebelo/metabolismo
Miosina Tipo V/metabolismo
-Fatores Etários
Cadáver
Eletroforese em Gel de Ágar
Immunoblotting
Imuno-Histoquímica
Limites: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Criança
Pré-Escolar
Feminino
Seres Humanos
Lactente
Recém-Nascido
Masculino
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  2 / 7 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Zanoni, Jacqueline Nelisis
Texto completo
Id: lil-660308
Autor: Tronchini, Eleandro Aparecido; Trevizan, Aline Rosa; Tashima, Cristiano Massao; Pereira, Renata Virginia Ferreira; Zanoni, Jacqueline Nelisis.
Título: Supplementation with 0.1% and 2% vitamin e in diabetic rats: analysis of myenteric neurons immunostained for myosin-V and nNOS in the jejunum / Suplementação com vitamina E 0,1% e 2% em ratos diabéticos: análise de neurônios mioentéricos imunomarcados para miosina-V e nNOS no jejuno
Fonte: Arq. gastroenterol;49(4):284-290, Oct.-Dec. 2012. ilus, graf, tab.
Idioma: en.
Resumo: CONTEXT: Diabetes mellitus is a disease characterized by hyperglycemia that, when allowed to progress long-term untreated, develops vascular and neurological complications, which are responsible for the development of alterations in the enteric nervous system in diabetic patients. In the gastrointestinal tract, diabetes mellitus promotes motor and sensory changes, and in the reflex function of this system, causing gastroparesis, diarrhea, constipation, megacolon, slow gastrointestinal transit, gastric stasis and dilation with decreased or increased peristaltic contractions. Several studies have shown that oxidative stress is the main responsible for the vascular and neurological complications affecting the enteric nervous system of diabetics. OBJECTIVE: The effects of 0.1% and 2% vitamin E on myosin-V- and nNOS-immunoreactive neurons in the jejunum of diabetic rats were investigated. METHODS: Thirty rats were divided into the groups: normoglycemic, normoglycemic treated with 0.1% vitamin E, normoglycemic treated with 2% vitamin E, diabetic, diabetic treated with 0.1% vitamin E, and diabetic treated with 2% vitamin E. The neuronal density and areas of neuron cell bodies were determined. RESULTS: Diabetes (diabetic group) significantly reduced the number of myosin-V-immunoreactive neurons compared with the normoglycemic group. The diabetic treated with 0.1% vitamin E and diabetic treated with 2% vitamin E groups did not exhibit a greater density than the D group (P>0.05). Nitrergic density did not change with diabetes (P>0.05). The areas of myosin-V- and nNOS-immunoreactive neurons significantly increased in the normoglycemic treated with 2% vitamin E and diabetic groups compared with the normoglycemic group. CONCLUSION: Supplementation with 2% vitamin E had a neurotrophic effect only in the area of myosin-V-immunoreactive neurons compared with the diabetic group.

CONTEXTO: O diabetes mellitus (DM) é uma doença caracterizada pela hiperglicemia que a longo prazo, quando não tratada, desenvolve complicações vasculares e neurológicas, responsáveis pelo desenvolvimento das alterações no sistema nervoso entérico de pacientes diabéticos. Em nível gastrointestinal o DM provoca modificações motoras, sensoriais e na função reflexa desse sistema, podendo ocasionar gastroparesia, diarreia, constipação, megacólon, lentidão do trânsito gastrointestinal, estase e dilatação gástrica com diminuição ou aumento de contrações peristálticas. Diversos estudos têm evidenciado que o estresse oxidativo é o principal responsável pelas complicações vasculares e neurológicas que atingem o sistema nervoso entérico de diabéticos. OBJETIVO: O efeito da vitamina E 0,1% e 2 sobre a miosina-V e nNOS imunorreativas em neurônios do jejuno de ratos diabéticos foram investigados. MÉTODOS: Trinta ratos foram divididos em grupos: normoglicêmicos (NU), normoglicêmicos tratados com vitamina E 0,1% (NE1), normoglicêmicos tratados com vitamina E 2% (NE2), diabético (UD), diabéticos tratados com vitamina E 0,1% (DE1), e diabéticos tratados com vitamina E 2% (DE2). A densidade neuronal e áreas de corpos celulares de neurônios foram determinadas. RESULTADOS: Diabetes (UD grupo) reduziu significativamente o número de neurônios miosina-V imunorreativos quando comparado com o grupo UN. Os grupos DE1 e DE2 não exibem uma maior densidade do que o grupo D (P>0,05). Densidade nitrérgicos não se alterou com diabetes (P>0,05). As áreas dos neurônios miosina-V e nNOS imunorreativos aumentaram significativamente nos grupos NE2 e UD comparados com o grupo UN. CONCLUSÃO: A suplementação com vitamina E 2% teve um efeito neurotrófico apenas na área da miosina-V imunorreativos neurônios em comparação com o grupo UD.
Descritores: Diabetes Mellitus Experimental/metabolismo
Jejuno/inervação
Plexo Mientérico/química
Miosina Tipo V/análise
Óxido Nítrico Sintase Tipo I/análise
Vitamina E/administração & dosagem
Vitaminas/administração & dosagem
-Diabetes Mellitus Experimental/complicações
Diabetes Mellitus Experimental/patologia
Imuno-Histoquímica
Jejuno/química
Miosina Tipo V/efeitos dos fármacos
Neurônios/química
Neurônios/efeitos dos fármacos
Óxido Nítrico Sintase Tipo I/efeitos dos fármacos
Ratos Wistar
Estreptozocina
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


  3 / 7 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Zanoni, Jacqueline Nelisis
Texto completo
Id: lil-599655
Autor: Souza, Ivan Domicio da Silva; Ribeiro, Janine Silva; Bersani-Amado, Ciomar Aparecida; Zanoni, Jacqueline Nelisis.
Título: Analysis of myosin-V immunoreactive myenteric neurons from arthritic rats / Análise dos neurônios mioentéricos miosina-V imunoreativos de ratos artríticos
Fonte: Arq. gastroenterol;48(3):205-210, July-Sept. 2011. ilus, graf, tab.
Idioma: en.
Resumo: CONTEXT: The inflammatory response itself and the consequent oxidative stress are able to promote neurodegeneration. So, it is possible that enteric nervous system is affected by inflammatory diseases threatening quality of life of patients. However, gastrointestinal symptoms of arthritis are usually attributed to anti-inflammatory drugs rather than neural damage. OBJECTIVE: To confirm if the general population of myenteric neurons from the ileum and jejunum of rats is affected by arthritis. METHODS: Twenty Holtzmann rats, 58-day-old male, were used and divided in four groups: control group (C30), arthritic group (Art30), older control group (C60) and older arthritic group (Art60). At 58 days old, the animals in groups Art30 and Art60 received an injection of the complete Freund's adjuvant in order to induce arthritis. The whole-mount preparations of ileum and jejunum were processed for myosin-V immunohistochemistry. Quantitative and morphometric analyses were performed. RESULTS: Groups Art30 and Art60 presented, respectively, a reduction of 2 percent and 6 percent in intestinal area when compared to their control groups. No significant differences were observed in general neuronal density among the four groups (P>0.05). Group C60 presented a reduction of 14.4 percent and 10.9 percent in mean neuronal cell body area when compared to group C30 (P<0.05), for the ileum and jejunum, respectively. The other groups had a similar mean neuronal cell body area (P>0.05). CONCLUSION: Arthritis does not promote quantitative or morphological damages in general myenteric population. However, studies in progress have revealed some significant alterations in myenteric neurons subpopulations (nitrergic and VIP-ergic neurons).

CONTEXTO: A resposta inflamatória e o estresse oxidativo acentuados em decorrência da artrite reumatóide são capazes de promover neurodegeneração. Nessas condições, é possível que o sistema nervoso entérico seja afetado, diminuindo a qualidade de vida dos pacientes. No entanto, os sintomas da artrite no trato gastrointestinal são geralmente associados ao uso de medicamentos anti-inflamatórios do que a um possível dano neural. OBJETIVO: Verificar se a população geral de neurônios mioentéricos do íleo e do jejuno de ratos artríticos é afetada pela artrite. MÉTODOS: Foram utilizados 20 ratos Holtzmann, inicialmente com 58 dias de idade, divididos em 4 grupos: controle com 88 dias (C30); artrítico com 88 dias (Art30); controle com 118 dias (C60) e artrítico com 118 dias (Art60). Os animais dos grupos Art30 e Art60 receberam aos 58 dias de idade o adjuvante completo de Freund para indução da artrite. Os preparados totais de íleo e jejuno foram submetidos a imunoistoquímica para a proteína miosina-V. Realizou-se análises quantitativas e morfométricas dos neurônios. RESULTADOS: Os animais Art30 e Art60 apresentaram, respectivamente, redução de 2 por cento e 6 por cento na área intestinal em relação aos seus controles. Não foram observadas diferenças na densidade neuronal geral entre os quatro grupos (P>0,05). O grupo C60 apresentou redução de 14,4 por cento e 10,9 por cento na área média do corpo celular neuronal em relação ao grupo C30 (P<0,05). Os demais grupos apresentaram área média de corpo celular semelhante (P>0,05). CONCLUSÃO: A artrite não provocou alterações quantitativas ou morfológicas na população mioentérica geral, entretanto, estudos em andamento revelam alterações significativas em subpopulações de neurônios mioentéricos (nitrérgicos e VIP-érgicos).
Descritores: Artrite/patologia
Íleo/inervação
Jejuno/inervação
Plexo Mientérico/patologia
Miosina Tipo V/análise
Neurônios/química
-Biomarcadores/análise
Imuno-Histoquímica
Íleo/patologia
Jejuno/patologia
Neurônios/patologia
Ratos Sprague-Dawley
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


  4 / 7 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Larson, R. E
Texto completo
Id: lil-556861
Autor: Brinn, L. S; Pereira Leite, J; Larson, R. E; Martins, A. R.
Título: Differential patterns of myosin Va expression during the ontogenesis of the rat hippocampus
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;43(9):890-898, Sept. 2010. ilus.
Idioma: en.
Resumo: Myosin Va is an actin-based, processive molecular motor protein highly enriched in the nervous tissue of vertebrates. It has been associated with processes of cellular motility, which include organelle transport and neurite outgrowth. The in vivo expression of myosin Va protein in the developing nervous system of mammals has not yet been reported. We describe here the immunolocalization of myosin Va in the developing rat hippocampus. Coronal sections of the embryonic and postnatal rat hippocampus were probed with an affinity-purified, polyclonal anti-myosin Va antibody. Myosin Va was localized in the cytoplasm of granule cells in the dentate gyrus and of pyramidal cells in Ammon's horn formation. Myosin Va expression changed during development, being higher in differentiating rather than already differentiated granule and pyramidal cells. Some of these cells presented a typical migratory profile, while others resembled neurons that were in the process of differentiation. Myosin Va was also transiently expressed in fibers present in the fimbria. Myosin Va was not detected in germinative matrices of the hippocampus proper or of the dentate gyrus. In conclusion, myosin Va expression in both granule and pyramidal cells showed both position and time dependency during hippocampal development, indicating that this motor protein is under developmental regulation.
Descritores: Hipocampo/embriologia
Hipocampo/metabolismo
Miosina Tipo V/análise
-Giro Denteado/embriologia
Giro Denteado/metabolismo
Imuno-Histoquímica
Miosina Tipo V/metabolismo
Células Piramidais/embriologia
Células Piramidais/metabolismo
Ratos Wistar
Limites: Animais
Feminino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  5 / 7 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: lil-548024
Autor: Calábria, Luciana Karen; Teixeira, Renata Roland; Coelho Gonçalves, Sybelli Magda; Passos Lima, Andreia Barcelos; Santos, Ana Alice Diniz dos; Martins, Antônio Roberto; Espindola, Foued Salmen.
Título: Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay
Fonte: Biol. Res;43(1):7-12, 2010. ilus.
Idioma: en.
Resumo: The present study compared two heating methods currently used for antigen retrieval (AR) immunostaining: the microwave oven and the steam cooker. Myosin-V, a molecular motor involved in vesicle transport, was used as a neuronal marker in honeybee Apis mellifera brains fixed in formalin. Overall, the steam cooker showed the most satisfactory AR results. At 100 ºC, tissue morphology was maintained and revealed epitope recovery, while evaporation of the AR solution was markedly reduced; this is important for stabilizing the sodium citrate molarity of the AR buffer and reducing background effects. Standardization of heat-mediated AR of formalin-fixed and paraffin-embedded tissue sections results in more reliable immunostaining of the honeybee brain.
Descritores: Antígenos/análise
Abelhas/imunologia
Imuno-Histoquímica/métodos
Miosina Tipo V/análise
Lobo Óptico de Animais não Mamíferos/química
-Antígenos/imunologia
Calefação
Micro-Ondas
Inclusão em Parafina
Coloração e Rotulagem
Limites: Animais
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  6 / 7 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-491535
Autor: Schoffen, J. P; Natali, M. R.
Título: Effect of age on the myosin-V immunoreactive myenteric neurons of rats ileum
Fonte: Biocell;31(1):33-39, abr. 2007. ilus, tab, graf.
Idioma: en.
Resumo: Alterations in the gastrointestinal neuromuscular function related to age have been demonstrated in human and animal models. This study analyzes the effects of the aging process on the area of the neuronal cell bodies of the myenteric plexus in the antimesenteric and intermediate regions of the ileal circumference of Wistar, 12 month-old in comparison 3 month-old animals. The ileum was removed and whole-mount preparations immunostained by the antibody anti-myosin-V were processed. The morphometric analyses were performed using a computerized image analysis system, with a subsequent distribution of neurons by size in intervals of 100 micro2. The cellular body morphometry revealed a significant increase in the size of the myosin-V- immunoreactive myenteric neurons from 12 month-old animals when compared with 3 month –old animals. However, significant differences between the regions were not observed; these observations were not age-dependent. The implications of these results in relation to the increase of the body weight, size of the small intestine, general organization of the myenteric plexus, staining method of neurons and the possible factors involved in the regulation and/or control of the volume of neronal cells due to aging, are discussed.
Descritores: Envelhecimento
Íleo/inervação
Miosina Tipo V/análise
Miosina Tipo V/imunologia
Neurônios/citologia
Neurônios/química
Plexo Mientérico/citologia
-Imuno-Histoquímica
Ratos Wistar
Limites: Animais
Masculino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo


  7 / 7 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-468028
Autor: Aversi-Ferreira, Tales Alexandre; Penha-Silva, Nilson; Santos, James Ferreira dos; Almeida, Antônio Wilson; Espíndola, Foued Salmen.
Título: Immunoelectronic localization of myosin-Va in rat cerebellum
Fonte: Braz. j. morphol. sci = Rev. bras. ciênc. morfol;22(4):211--214, Oct.-Dec. 2005. ilus.
Idioma: en.
Resumo: Myosin V is an unconventional type of actin-binding myosin that participates in cytoplasmic organelle transport. Although this unconventional myosin has been extensively studied, its subcellular localization in the mammalian cerebellum has not been determined. In this work, we used an antibody against the tail domain of the myosin-Va heavy chain and a secondary antibody labeled with protein A-gold (15 nm) to study the subcellular distribution of this protein. Myosin-Va was found in the cytoplasm, where it was associated with a filament (probably actin). This protein was also detected in the plasma membrane of axons and dendrites in the molecular layer in rat cerebellum.
Descritores: Células de Purkinje/fisiologia
Miosina Tipo V/química
Fenômenos Fisiológicos do Sistema Nervoso
Proteína Estafilocócica A
Telencéfalo
-Células de Purkinje/citologia
Ratos Wistar
Limites: Animais
Adulto
Ratos
Responsável: BR734.1 - Biblioteca Central Cesar Lattes - BCCL



página 1 de 1
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde