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Texto completo SciELO Chile
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Id: biblio-1011398
Autor: Aramwit, Pornanong; Luplertlop, Natthanej; Kanjanapruthipong, Tapanee; Ampawong, Sumate.
Título: Effect of urea-extracted sericin on melanogenesis: potential applications in post-inflammatory hyperpigmentation
Fonte: Biol. Res;51:54, 2018. graf.
Idioma: en.
Projeto: Thailand Research Fund (TRF); . Office of the Higher Education Commission (OHEC).
Resumo: BACKGROUND: Hyperpigmentation disorders such as post-inflammatory hyperpigmentation are major concerns not only in light-skinned people but also in Asian populations with darker skin. The anti-tyrosinase and immunomodulatory effects of sericin have been known for decades. However, the therapeutic effects of sericin on hyperpigmentation disorders have not been well documented. METHODS: In this study, we used an in vitro model to study the anti-tyrosinase, tolerogenic, and anti-melanogenic effects of sericin on Staphylococcus aureus peptidoglycan (PEG)-stimulated melanocytes, dendritic cells (DCs), and artificial skin (MelanoDerm™). Enzyme-linked immunosorbent assay, conventional and immunolabeled electron microscopy, and histopathological studies were performed. RESULTS: The results revealed that urea-extracted sericin has strong anti-tyrosinase properties as shown by a reduction of tyrosinase activity in melanin pigments both 48 h and 10 days after allergic induction with PEG. Anti-inflammatory cytokines including interleukin (IL)-4, IL-10, and transforming growth factor-p were upregulated upon sericin treatment (10, 20, and 50 µg/mL), whereas production of allergic chemokines, CCL8 and CCL18, by DCs was diminished 48 h after allergic induction with PEG. Moreover, sericin lowered the expression of micropthalmia-associated transcription factor (MITF), a marker of melanogenesis regulation, in melanocytes and keratinocytes, which contributed to the reduction of melanin size and the magnitude of melanin deposition. However, sericin had no effect on melanin transport between melanocytes and keratinocytes, as demonstrated by a high retention of cytoskeletal components. CONCLUSION: In summary, sericin suppresses melanogenesis by inhibition of tyrosinase activity, reduction of inflammation and allergy, and modulation of MITF function.
Descritores: Queratinócitos/efeitos dos fármacos
Monofenol Mono-Oxigenase/antagonistas & inibidores
Hiperpigmentação/tratamento farmacológico
Melanócitos/efeitos dos fármacos
-Fatores de Transcrição/efeitos dos fármacos
Microscopia Eletrônica
Transdução de Sinais/efeitos dos fármacos
Células Cultivadas
Fator de Transcrição Associado à Microftalmia
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central

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Texto completo SciELO Chile
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Id: lil-626746
Autor: Kaewkorn, Waraporn; Limpeanchob, Nanteetip; Tiyaboonchai, Waree; Pongcharoen, Sutatip; Sutheerawattananonda, Manote.
Título: Effects of silk sericin on the proliferation and apoptosis of colon cancer cells
Fonte: Biol. Res;45(1):45-50, 2012. ilus, tab.
Idioma: en.
Projeto: Thailand Research Fund; . Naresuan University. Faculty of Medicine; . Naresuan University.
Resumo: Sericin is a silk protein woven from silkworm cocoons (Bombyx mori). In animal model, sericin has been reported to have anti-tumoral action against colon cancer. The mechanisms underlying the activity of sericin against cancer cells are not fully understood. The present study investigated the effects of sericin on human colorectal cancer SW480 cells compared to normal colonic mucosal FHC cells. Since the size of the sericin protein may be important for its activity, two ranges of molecular weight were tested. Sericin was found to decrease SW480 and FHC cell viability. The small sericin had higher anti-proliferative effects than that of the large sericin in both cell types. Increased apoptosis of SW480 cells is associated with increased caspase-3 activity and decreased Bcl-2 expression. The anti-proliferative effect of sericin was accompanied by cell cycle arrest at the S phase. Thus, sericin reduced SW480 cell viability by inducing cell apoptosis via caspase-3 activation and down-regulation of Bcl-2 expression. The present study provides scientific data that support the protective effect of silk sericin against cancer cells of the colon and suggests that this protein may have significant health benefits and could potentially be developed as a dietary supplement for colon cancer prevention.
Descritores: Apoptose/efeitos dos fármacos
Proliferação de Células/efeitos dos fármacos
Colo/efeitos dos fármacos
Neoplasias do Colo/patologia
Linhagem Celular Tumoral
Sobrevivência Celular
Neoplasias do Colo/metabolismo
Neoplasias do Colo/prevenção & controle
Mucosa Intestinal/citologia
Mucosa Intestinal/efeitos dos fármacos
Peso Molecular
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central

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