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Pesquisa : D05.750.716.195 [Categoria DeCS]
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Texto completo SciELO Brasil
Texto completo
Id: biblio-1132209
Autor: Mamani, Hulda Noemi Chambi; Graziano, Juliana Augusti; Lacerda, Roseli Sengling; Bittante, Ana Mônica Quinta Barbosa; Gomide, Catarina Abdalla; Sobral, Paulo José do Amaral.
Título: Castor Bean Cake Protein-based Biodegradable Films: Gallic Acid Effect
Fonte: Braz. arch. biol. technol;63:e20190141, 2020. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Abstract: The objective of this work was to evaluate the effect of gallic acid (GA) concentration on some physical properties and biodegradability of films produced with proteins extracted from the castor bean cake. The films, prepared by the casting technique, showed homogeneous and brownish appearance. As the GA concentration increased (from 0 to 10 g/100 g protein), the films gradually became darker and more opaque; while the gloss had few significant differences. Solubility, tensile strength and elasticity modulus values of films varied due to changing concentrations of gallic acid. Elongation at break and water vapor permeability values did not have significant changes. A 60% mineralization value of the film containing GA was obtained at 21 days, evidencing its biodegradability. These dark and opaque films could be used in agriculture, specifically in seedling bags as the dark color decrease the incidence of light, preventing root weakening, and the seedlings can be transplanted directly without removal of the film.
Descritores: Ricinus/química
Plásticos Biodegradáveis/química
Ácido Gálico/análise
-Fenômenos Físicos
Responsável: BR1.1 - BIREME

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Texto completo SciELO Brasil
Texto completo
Id: lil-723097
Autor: Naheed, Nighat; Jamil, Nazia.
Título: Optimization of biodegradable plastic production on sugar cane molasses in Enterobacter sp. SEL2
Fonte: Braz. j. microbiol;45(2):417-426, Apr.-June 2014. graf, tab.
Idioma: en.
Resumo: Contaminated environments have a large number of bacteria which can accumulate PHA as their energy reserves. Out of 54 isolated bacterial strains from three groups of contaminated sites 48 were found PHA positive. The sites were grouped on the basis of the type of carbon sources i.e. sugars, fatty acids and much diverse type. Strains MFD5, MFD11, UML3, USL2, SEL2, SEL3, SEL10 and PFW1 produced 69.9 ± 0.29, 75.27 ± 0.45, 65.43 ± 0.1, 72.54 ± 0.27, 76.61 ± 0.28, 61.81 ± 0.05, 71.16 ± 0.09 and 74.92 ± 0.5 percent of PHA to their constant cell weight (CCW) respectively in PHA detection media supplemented with 2% glucose. Molasses, whey, crumbs hydrolysate and palm oil were checked as inexpensive carbon sources. Molasses alone could supply the required nutrients for growth and PHA production. Strain SEL2 produced 47.36 ± 0.45% PHA using 2% molasses at 37 °C and pH 7.0. Upon production optimization the best accumulation (80.95 ± 0.01%) was observed in PHA detection media with 0.2% nitrogen source, 3% molasses, pH 5.0 and 37 °C by the strain SEL2. The overall effect of the presence of increased molasses concentration in the media was positive it increased the accumulation period till 72 h. Enterobacter sp. SEL2 (JF901810) is first time being reported for PHA production.
Descritores: Plásticos Biodegradáveis/metabolismo
Microbiologia Ambiental
Meios de Cultura/química
Enterobacter/isolamento & purificação
Concentração de Íons de Hidrogênio
Responsável: BR1.1 - BIREME

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Texto completo SciELO Chile
Texto completo
Id: lil-640552
Autor: Krueger, Cristhiane L; Radetski, Claudemir M; Bendia, Amanda G; Oliveira, Ida M; Castro-Silva, Marcus A; Rambo, Carlos R; Antonio, Regina V; Lima, André O. S.
Título: Bioconversion of cassava starch by-product into bacillus and related bacteria polyhydroxyalkanoates
Fonte: Electron. j. biotechnol;15(3):8-8, May 2012. ilus, tab.
Idioma: en.
Projeto: The Brazilian National Council for Scientific and Technological Development; . Brazilian Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Resumo: Background: Unlike petroleum-based synthetic plastics, biodegradable biopolymer generation from industrial residue is a key strategy to reduce costs in the production process, as well as in the waste management, since efficient industrial wastewater treatment could be costly. In this context, the present work describes the prospection and use of bacterial strains capable to bioconvert cassava starch by-product into biodegradable polyhydroxyalkanoates (PHAs). Results: The first step of this study was the bacterial competence screening which was conducted with 72 strains covering 21 Bacillus and related species. The microorganism growth in a medium with a starch substrate was measured by an innovative MTT assay, while the ability of the bacteria to secrete amylase and produce PHA was evaluated by the Nile Red Dye method. Based on growth and potential for PHA production, four isolates were selected and identified as Bacillus megaterium by 16S rRNA sequencing. When cultivated in hydrolyzed cassava starch by-product, maximum production reached 4.97 g dry biomass/L with 29.7 percent of Poly-(3-hydroxybutyrate) (characterized by FTIR). Conclusions: MTT assay proved to be a reliable methodology for monitoring bacterial growth in insoluble media. Selected amylolytic strains could be used as an alternative industrial process for biodegradable plastics production from starchy residues, reducing costs for biodegradable biopolymer production and wastewater treatment operations.
Descritores: Bacillus megaterium
Plásticos Biodegradáveis
Sais de Tetrazólio
Responsável: CL1.1 - Biblioteca Central

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