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Id: biblio-1056389
Autor: Ottone, Nicolás Ernesto; Guerrero, Marco; Alarcón, Eduardo; Navarro, Pablo.
Título: Statistical analysis of shrinkage levels of human brain slices preserved by sheet plastination technique with polyester resin / Análisis estadístico de los niveles de retracción de cortes de cerebro humano conservados mediante la técnica de plastinación de cortes con resina poliéster
Fonte: Int. j. morphol;38(1):13-16, Feb. 2020. tab.
Idioma: en.
Resumo: Plastination is currently the most important anatomical preservation technique due to the possibility of preserving bodies and organs for an indefinite period, in a dry and biosecure form, while preserving the morphological characteristics of the tissues. However, the shrinkage of the samples is also part of the plastination, perhaps becoming one of its few disadvantages. This paper presents the shrinkage caused by the classic technique of sheet plastination with polyester resin (Biodur® P40) in human brain slices, with the aim of statistically establishing the percentages of tissue shrinkage caused by this plastination protocol.

La plastinación es actualmente la técnica de preservación anatómica más importante debido a la posibilidad de preservar los cuerpos y órganos por un período indefinido, en forma seca y biosegura, al tiempo que preserva las características morfológicas de los tejidos. Sin embargo, la retracción de las muestras también es parte de la plastinación, quizás convirtiéndose en una de sus pocas desventajas. Este artículo presenta la retracción causada por la técnica clásica de plastinación de cortes con resina poliéster (Biodur® P40) en cortes de cerebro humano, con el objetivo de establecer estadísticamente los porcentajes de retracción de tejidos causados por este protocolo de plastinación.
Descritores: Tamanho do Órgão
Poliésteres/química
Encéfalo/anatomia & histologia
Inclusão em Plástico/métodos
-Preservação de Tecido
Análise Estatística
Resinas
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1054681
Autor: Nascimento, Lízia Daniela e Silva; Nicolau, Renata Amadei; Maia Filho, Antônio Luiz Martins; Santos, José Zilton Lima Verde; Fonseca, Khetyma Moreira; Ferreira, Danniel Cabral Leão; Sousa, Rayssilane Cardoso de; Viana, Vicente Galber Freitas; Carvalho, Luiz Fernando Meneses; Figueredo-Silva, José.
Título: Effect of norbixin-based poly(hydroxybutyrate) membranes on the tendon repair process after tenotomy in rats
Fonte: Acta cir. bras;34(11):e201901101, Nov. 2019. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To determine the efficacy of norbixin-based poly(hydroxybutyrate) (PHB) membranes for Achilles tendon repair. Methods: Thirty rats were submitted to total tenotomy surgery of the right Achilles tendon and divided into two groups (control and membrane; n = 15 each), which were further subdivided into three subgroups (days 7, 14, and 21; n = 5 each). Samples were analyzed histologically. Results: Histological analysis showed a significant reduction in inflammatory infiltrates on days 7, 14 (p < 0.0001 for both), and 21 (p = 0.0004) in the membrane group compared to that in the control group. There was also a significant decrease in the number of fibroblasts in the control group on days 7, 14 (p < 0.0001), and 21 (p = 0.0032). Further, an increase in type I collagen deposition was observed in the membrane group compared to that in the control group on days 7 (p = 0.0133) and 14 (p = 0.0107). Conclusion: Treatment with norbixin-based PHB membranes reduces the inflammatory response, increases fibroblast proliferation, and improves collagen production in the tendon repair region, especially between days 7 and 14.
Descritores: Poliésteres/farmacologia
Tendão do Calcâneo/cirurgia
Tendão do Calcâneo/efeitos dos fármacos
Carotenoides/farmacologia
Tenotomia/métodos
Hidroxibutiratos/farmacologia
-Valores de Referência
Regeneração/efeitos dos fármacos
Tendão do Calcâneo/patologia
Reprodutibilidade dos Testes
Ratos Wistar
Colágeno Tipo I/análise
Colágeno Tipo I/efeitos dos fármacos
Colágeno Tipo III/análise
Colágeno Tipo III/efeitos dos fármacos
Fibroblastos/efeitos dos fármacos
Limites: Humanos
Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-973505
Autor: Biondo-Simões, Maria de Lourdes Pessole; Sichciopi, Arthur Andrade; Ioshii, Sérgio Ossamu; Robes, Rogério Ribeiro; Biondo-Simões, Rachel.
Título: Comparative study of fibrosis induced by Marlex®, Parietex Composite®, Vicryl® and Ultrapro® meshes1
Fonte: Acta cir. bras;33(9):792-798, Sept. 2018. graf.
Idioma: en.
Resumo: Abstract Purpose: To evaluate the fibrosis induced by four different meshes: Marlex®, Parietex Composite®, Vicryl® and Ultrapro®. Methods: Histological cutouts of abdominal wall were analyzed with polarized light 28 days after the meshes implants and colorized by picrosirius to identify the intensity of collagen types I and III, and their maturation index. Results: When the four groups were compared, the total collagen area analyzed was bigger in groups A and D, with no difference between them. The collagen type I density was bigger in group A, with an average of 9.62 ± 1.0, and smaller in group C, with an average of 3.86 ± 0.59. The collagen type III density was similar in groups A, B and C, and bigger in group D. The collagen maturation index was different in each of the four groups, bigger in group A with 0.87, group B with 0.66, group D with 0.57 and group C with 0.33 (p = 0.0000). Conclusion: The most prominent fibrosis promotion in the given meshes was found on Marlex® (polypropylene mesh) and the Parietex Composite® (non-biodegradable polyester); the collagen maturation index was higher in the Marlex® mesh, followed by Ultrapro®, Parietex Composite® and Vicryl® meshes.
Descritores: Poliésteres/efeitos adversos
Poliglactina 910/efeitos adversos
Polipropilenos/efeitos adversos
Telas Cirúrgicas/efeitos adversos
Colágeno/efeitos adversos
Parede Abdominal/patologia
-Poliésteres/administração & dosagem
Poliglactina 910/administração & dosagem
Polipropilenos/administração & dosagem
Fatores de Tempo
Fibrose/etiologia
Fibrose/patologia
Teste de Materiais
Aderências Teciduais/etiologia
Aderências Teciduais/patologia
Colágeno/administração & dosagem
Modelos Animais
Parede Abdominal/cirurgia
Limites: Animais
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: biblio-973478
Autor: Lima, Roberta Junqueira de; Schnaider, Taylor Brandão; Francisco, Antônio Marcos Coldibelli; FrancescatoVeiga, Daniela.
Título: Absorbable suture. Best aesthetic outcome in cesarian scar
Fonte: Acta cir. bras;33(11):1027-1036, Nov. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To compare two suture threads, poliglecaprone 25 and nylon, used as intradermal suture for skin closure in women undergoing their first cesarean section. Methods: This is a randomized clinical trial. A total of 60 women undergoing their first cesarean section were enrolled and prospectively assessed. They were randomly allocated to group I (n=30), which received an intradermal suture with nylon 4.0 or to group II (n=30), which had an intradermal suture with poliglecaprone 25, 4.0. The main author took standardized photographs of the scar 6 months after the operation. Four independent raters, two senior obstetricians and two senior plastic surgeons (a male and a female physician from each specialty) assessed the photographs.The panelists rated the scar according to Trimbos scale, composed by the subscales hypertrophy, color and width of the scar. Results: At baseline, patients in both groups were similar regarding age and body mass index. Five patients withdraw the study, four from group and one from group II. Scars of patients from group II were significantly less hypertrophic (p=0.001), thinner (p=0.019) and had more acceptable color (p=0.019). Conclusion: The intradermal suture with poliglecaprone 25 for skin closure after cesarean incision provides better aesthetic result.
Descritores: Poliésteres/uso terapêutico
Suturas
Cesárea/métodos
Técnicas de Sutura
Cicatriz
Dioxanos/uso terapêutico
Nylons
-Valores de Referência
Materiais Biocompatíveis
Estudos Prospectivos
Reprodutibilidade dos Testes
Resultado do Tratamento
Estatísticas não Paramétricas
Estética
Limites: Humanos
Feminino
Tipo de Publ: Ensaio Clínico Controlado Aleatório
Responsável: BR1.1 - BIREME


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Torres, Orlando Jorge Martins
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Id: biblio-1019263
Autor: Ribeiro, Waston Gonçalves; Rodrigues, Diego Vinnicyus Santos; Atta, Francisco Felipe Moreira; Ramos, Izabelle Smith Frazão; Frazão, Fabiola Nassar Sousa; Torres, Orlando Jorge Martins; Pitombo, Marcos Bettini.
Título: Comparative study of peritoneal adhesions after intraperitoneal implantation in rats of meshes of polypropylene versus polypropylene/polyglecaprone versus polyester/porcine collagen
Fonte: Acta cir. bras;34(6):e201900603, 2019. tab, graf.
Idioma: en.
Resumo: Abstract Purpose To Compare the extent and intensity of adhesions formed between the intra-abdominal organs and the intraperitoneal implants of polypropylene mesh versus polypropylene/polyglecaprone versus polyester/porcine collagen used for correction of abdominal wall defect in rats. Methods After the defect in the abdominal wall, thirty Wistar rats were placed in three groups (ten animals each) for intraperitoneal mesh implant: polypropylene group, polypropylene/polyglecaprone group, and polyester/porcine collagen group. The macroscopic evaluation of the extent and intensity of adhesions was performed 21 days after the implant. Results The polypropylene group had a higher statistically significant impairment due to visceral adhesions (p value = 0.002) and a higher degree of intense adherence in relation to polypropylene/polyglecaprone and polyester/porcine collagen groups (p value<0.001). The polyester/porcine collagen group showed more intense adhesions than the polypropylene/polyglecaprone group (p value=0.035). Conclusions The intraperitoneal implantation of polypropylene meshes to correct defects of the abdominal wall caused the appearance of extensive and firm adhesions to intra-abdominal structures. The use of polypropylene/polyglecaprone or polyester/porcine collagen tissue-separating meshes reduces the number and degree of adhesions formed.
Descritores: Doenças Peritoneais/etiologia
Poliésteres/administração & dosagem
Polipropilenos/administração & dosagem
Telas Cirúrgicas/efeitos adversos
Aderências Teciduais/etiologia
Colágeno/administração & dosagem
Dioxanos/administração & dosagem
-Poliésteres/efeitos adversos
Polipropilenos/efeitos adversos
Teste de Materiais
Colágeno/efeitos adversos
Ratos Wistar
Parede Abdominal/patologia
Dioxanos/efeitos adversos
Limites: Animais
Masculino
Ratos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: biblio-1040169
Autor: Guerrero, Marco; Vargas, Claudia; Alarcón, Eduardo; del Sol, Mariano; Ottone, Nicolás Ernesto.
Título: Desarrollo de un protocolo de plastinación de cortes con resina poliéster aplicado a secciones de cerebro humano / Development of a sheet plastination protocol with polyester resin applied to human brain slices
Fonte: Int. j. morphol;37(4):1557-1563, Dec. 2019. tab, graf.
Idioma: es.
Resumo: La plastinación es una técnica anatómica de conservación cadavérica creada en 1977 por Gunther von Hagens, en Heidelberg, Alemania, y que sustituye los líquidos biológicos y/o de fijación por acetona, para luego impregar las muestras con distintas resinas, dependiendo de la técnica de plastinación desarrollada, para finalmente llevar a cabo la polimerización de los componentes incorporados a las muestras, para obtener muestras biológicas secas y totalmente duraderas. El objetivo de este trabajo consistió en desarrollar un protocolo de plastinación de cortes con resina poliéster (Biodur® P40) en secciones de 3 mm de espesor de cerebro humano. La muestras fueron fijadas y conservadas con formalina al 10 %. Los cerebros luego fueron seccionados con una maquina cortadora de tejidos, obteniéndose láminas delgadas de 3 mm de espesor. Inmediatamente los cortes de cerebro fueron colocados en deshidratación en acetona al 100 %, a -25 ºC, durante 7 días el primer baño de acetona, y durante otros 3 días más, para el segundo baño de acetona. Una vez deshidratados los cortes, estos fueron colocados en resina poliéster Biodur® P40 y se llevó a cabo la impregnación forzada de los cortes, en cámara de vacío a temperatura ambiente (20 ºC). Una vez finalizada la impregnación forzada, se procedió a la etapa de curado, la cual en primer lugar consiste en el armado de las cámaras de curado dentro de las cuales se colocaran los cortes con resina poliéster. Las cámaras de curado fueron colocadas bajo luz UV para acelerar la polimerización del poliéster y finalizar el proceso de plastinación. Se logró desarrollar satisfactoriamente en el Laboratorio de Plastinación y Técnicas Anatómicas de la Universidad de La Frontera un protocolo de plastinación de cortes con resina poliéster, obteniendo una excelente conservación de cortes de cerebro, con diferenciación de sustancias gris y blanca, y conservación de todas las características morfológicas.

Plastination is an anatomical technique of cadaveric conservation created in 1977 by Gunther von Hagens, in Heidelberg, Germany, and that substitutes biological and / or fixation fluids with acetone, to then impregnate the samples with different resins, depending on the developed plastination technique, to finally carry out the polymerization of the components incorporated into the samples, to obtain dry and totally durable biological samples. The aim of this work was to develop a sheet plastination protocol with polyester resin (Biodur® P40) in 3 mm thick slices of human brain. The samples were fixed and preserved with 10 % formalin. The brains were sectioned with a slice cut machine, obtaining thin sheets of 3 mm thick. Immediately the slices of brain were placed in dehydration in 100 % acetone, at -25 °C, for 7 days the first acetone bath, and for another 3 more days, for the second acetone bath. Once the cuts were dehydrated, they were placed in Biodur® P40 polyester resin and the forced impregnation was carried out in a vacuum chamber at room temperature (20 °C). Once the forced impregnation was finished, the curing stage was carried out, which first consists in the assembly of the curing chambers within which the slices with polyester resin were placed. The curing chambers were placed under UV light to accelerate the polymerization of the polyester and finished the plastination process. A sheet plastination protocol with polyester resin was successfully developed in the Laboratory of Plastination and Anatomical Techniques of Universidad de La Frontera, obtaining excellent conservation of brain slices, with differentiation of gray and white substances, and conservation of all morphological characteristics.
Descritores: Poliésteres/química
Resinas Sintéticas/química
Encéfalo/anatomia & histologia
/métodos
FRUSTRATIONABDOMEN, ACUTE/métodos
-Protocolos Clínicos
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1018177
Autor: Salomón Torres, Alberto Dario.
Título: Masilla poliester para carrocerias metalicas.
Fonte: San Lorenzo; s.n; oct.1993. 93 p.
Idioma: es; en.
Tese: Apresentada a Facultad de Ciencias Quimicas para obtenção do grau de Texis.
Resumo: Es un estudio sobre la industrialización dela masilla poliester se basa principalmente en la reparación y acabado industrial además no requeire de una tecnologia muy complicada, pudiendo adquirirse en el mercado nacional
Descritores: Poliésteres
Responsável: PY37.1 - Biblioteca
6684225#Sol36m


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Id: lil-783797
Autor: Biondo-Simões, Maria de Lourdes Pessole; Morais, Camila Gomes de; Tocchio, Anna Flávia Zonato; Miranda, Renata Augusta de; Moura, Paula Almeida Pamponet; Colla, Kelly; Robes, Rogério Ribeiro; Ioshii, Sérgio Ossamu; Tomasich, Flávio Daniel Saavedra.
Título: Characteristics of the fibroplasia and collagen expression in the abdominal wall after implant of the polypropylene mesh and polypropylene/polyglecaprone mesh in rats
Fonte: Acta cir. bras;31(5):294-299, May 2016. graf.
Idioma: en.
Resumo: ABSTRACT PURPOSE: To compare fibroplasia and the resistance of the abdominal wall when polypropylene meshes and polypropylene/poliglecaprone are used. METHODS: Seventy-seven male Wistar rats were divided into three groups: Control Group (for resistance); Group E (polypropylene mesh); and Group U (polypropylene/poliglecaprone mesh). The animals in Groups E and U had a standard muscular and aponeurotic defect, with integral peritoneum, and correction with the mesh. Measurements were taken 4, 7, 14, 28 and 56 days after surgery. The resistance, and collagen density were studied. RESULTS: Resistance on the 56th day was similar in both meshes. The gain in resistance described an ascending curve for the polypropylene mesh and was irregular in the case of the polypropylene/poliglecaprone. Fibroplasia showed a gain in type I and type III collagen in both groups (p<0.001). Collagen III stabilized in the 14th day and collagen I continued to ascend. CONCLUSIONS: The gain in resistance of the polypropylene mesh is regular and ascending, whereas the polypropylene/poliglecaprone is not regular. The final resistance of both meshes is similar; the collagen density increases over time, and show the same inflammatory potential.
Descritores: Poliésteres/uso terapêutico
Polipropilenos/uso terapêutico
Colágeno/metabolismo
Parede Abdominal/cirurgia
Dioxanos/uso terapêutico
Hérnia Incisional/cirurgia
-Período Pós-Operatório
Resistência à Tração/fisiologia
Teste de Materiais/instrumentação
Ratos Wistar
Modelos Animais
Colágeno Tipo I/metabolismo
Colágeno Tipo III/metabolismo
Hérnia Incisional/fisiopatologia
Hérnia Incisional/metabolismo
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: biblio-837681
Autor: Biondo-Simões, Maria de Lourdes Pessole; Carvalho, Laísa Brandão; Conceição, Lucas Tavares; Santos, Kassyane Bordignon Piccinelli dos; Schiel, Wagner Augusto; Arantes, Mayara; Silveira, Tatiane da; Magri, Júlio César; Gomes, Fernanda Fabrini.
Título: Comparative study of Polypropylene versus Parietex composite®, Vicryl® and Ultrapro® meshes, regarding the formation of intraperitoneal adhesions
Fonte: Acta cir. bras;32(2):98-107, Feb. 2017. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To compare the polypropylene mesh (Marlex®) to Vicryl®, Parietex composite® and Ultrapro® meshes to assess the occurrence of adhesions in the intraperitoneal implantation. Methods: Sixty Wistar rats were allocated into three groups: PP+V, in which all the animals received a polypropylene and a Vicryl® mesh; PP+PC, with the implantation of polypropylene and Parietex composite® meshes and PP+UP, in which there was implantation of polypropylene and Ultrapro®. Macroscopic analysis was performed 28 days later to assess the percentage of mesh area affected by adhesion. Results: in the PP+ V group, the Vicryl® mesh showed lower adhesion formation (p=0.013). In the PP+PC, there were no differences between polypropylene and Parietex composite® (p=0.765). In the PP+UP group, Ultrapro® and polypropylene meshes were equivalent (p=0.198) . Conclusion: All the four meshes led to adhesions, with the Vicryl® mesh showing the least potential for its formation.
Descritores: Poliésteres
Poliglactina 910
Polipropilenos
Telas Cirúrgicas
Aderências Teciduais/prevenção & controle
Colágeno
Hérnia Ventral/cirurgia
-Próteses e Implantes
Materiais Biocompatíveis
Distribuição Aleatória
Ratos Wistar
Modelos Animais
Parede Abdominal/cirurgia
Limites: Animais
Masculino
Ratos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: biblio-1021543
Autor: Panyachanakul, Titiporn; Kitpreechavanich, Vichien; Tokuyama, Shinji; Krajangsang, Sukhumaporn.
Título: Poly(DL-lactide)-degrading enzyme production by immobilized Actinomadura keratinilytica strain T16-1 in a 5-L fermenter under various fermentation processes
Fonte: Electron. j. biotechnol;30:71-76, nov. 2017. graf, ilus, tab.
Idioma: en.
Projeto: National Research Council of Thailand.
Resumo: Background: Poly(DL-lactic acid), or PDLLA, is a biodegradable polymer that can be hydrolyzed by various types of enzymes. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported to have PDLLA depolymerase activity. However, few studies have reported on PDLLA-degrading enzyme production by bacteria. Therefore, the aims of this study were to determine a suitable immobilization material for PDLLA-degrading enzyme production and optimize PDLLA-degrading enzyme production by using immobilized A. keratinilytica strain T16-1 under various fermentation process conditions in a stirrer fermenter. Results: Among the tested immobilization materials, a scrub pad was the best immobilizer, giving an enzyme activity of 30.03 U/mL in a shake-flask scale. The maximum enzyme activity was obtained at aeration 0.25 vvm, agitation 170 rpm, 45°C, and 48 h of cultivation time. Under these conditions, a PDLLA-degrading enzyme production of 766.33 U/mL with 15.97 U/mL·h productivity was observed using batch fermentation in a 5-L stirrer fermenter. Increased enzyme activity and productivity were observed in repeated-batch (942.67 U/mL and 19.64 U/mL·h) and continuous fermentation (796.43 U/mL and 16.58 U/mL·h) at a dilution rate of 0.013/h. Scaled-up production of the enzyme in a 10-L stirrer bioreactor using the optimized conditions showed a maximum enzyme activity of 578.67 U/mL and a productivity of 12.06 U/mL·h. Conclusions: This research successfully scaled-up the enzyme production to 5 and 10 L in a stirrer fermenter and is helpful for many applications of poly(lactic acid).
Descritores: Poliésteres/metabolismo
Actinomycetales/enzimologia
Enzimas/biossíntese
-Biodegradação Ambiental
Reatores Biológicos
Enzimas/metabolismo
Enzimas Imobilizadas
Fermentação
Responsável: CL1.1 - Biblioteca Central



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