|
|
| Id: |
lil-762903
|
| Autor: |
Xu, B.L.; Zhao, Q.Z.; Gao, X.Y.; Hou, G.J.. |
| Título: |
Effect of estradiol and bisphenol A on human hepatoblastoma cell viability and telomerase activity |
| Fonte: |
Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;48(11):1004-1009, Nov. 2015. tab, graf.
|
| Idioma: |
en.
|
| Resumo: |
Sex hormones from environmental and physiological sources might play a major role in the pathogenesis of hepatoblastoma in children. This study investigated the effects of estradiol and bisphenol A on the proliferation and telomerase activity of human hepatoblastoma HepG2 cells. The cells were divided into 6 treatment groups: control, bisphenol A, estradiol, anti-estrogen ICI 182,780 (hereinafter ICI), bisphenol A+ICI, and estradiol+ICI. Cell proliferation was measured based on average absorbance using the Cell Counting-8 assay. The cell cycle distribution and apoptotic index were determined by flow cytometry. Telomerase activity was detected by polymerase chain reaction and a telomeric repeat amplification protocol assay. A higher cell density was observed in bisphenol A (P<0.01) and estradiol (P<0.05) groups compared with the control group. Cell numbers in S and G2/M phases after treatment for 48 h were higher (P<0.05), while the apoptotic index was lower (P<0.05) and telomerase activities at 48 and 72 h (P<0.05) were higher in these groups than in the control group. The cell density was also higher in bisphenol A+ICI (P<0.01) and estradiol+ICI (P<0.05) groups compared with the ICI group. Furthermore, cell numbers were increased in S and G2/M phases (P<0.05), while the apoptotic index was lower (P<0.05) and telomerase activities at 48 and 72 h were higher (P<0.05) in these groups than in the ICI group. Therefore, bisphenol A and estradiol promote HepG2 cell proliferation in vitro by inhibition of apoptosis and stimulation of telomerase activity via an estrogen receptor-dependent pathway.
|
| Descritores: |
Compostos Benzidrílicos/farmacologia
Proliferação de Células/efeitos dos fármacos
Estradiol/farmacologia
Antagonistas do Receptor de Estrogênio/farmacologia
Estrogênios não Esteroides/farmacologia
/efeitos dos fármacos
HEP GTEMEFOS CELLS/efeitos dos fármacos
Fenóis/farmacologia
Telomerase/efeitos dos fármacos
|
| |
-Apoptose/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Estradiol/análogos & derivados
Citometria de Fluxo
/enzimologia
HEP GTEMEFOS CELLS/enzimologia
Interfase/efeitos dos fármacos
Telomerase/metabolismo
|
| Limites: |
Humanos
|
| Responsável: |
BR1.1 - BIREME
|
|
