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Id: biblio-1150991
Autor: Figueira, Leticia.
Título: Papel de la adrenomedulina cerebelosa en la regulación de la presión arterial / Role of cerebelar adrenomedullin in blood pressure regulation.
Fonte: Caracas; s.n; oct. 2012. tab, ilus, graf. (Itf1482012615833).
Idioma: es.
Tese: Apresentada a Universidad Central de Venezuela. Facultad de Farmacia para obtenção do grau de Doctor.
Símbolo: Itf1482012615833.
Resumo: La adrenomedulina (AM) es un péptido ubicuo de 52 residuos de aminoácidos que cumple funciones importantes en la regulación de la función cardiovascular (CDV). La AM ejerce sus acciones a través de su unión con tres subtipos de receptores, el receptor del péptido relacionado al gen de la calcitonina tipo 1 (CGRP1), el receptor de AM tipo 1 (AM1) y tipo 2 (AM2). El CGRP1 está formado por el receptor similar al receptor de calcitonina (CRLR) y la proteína que modifica la actividad del receptor tipo 1 (RAMP1). El AM1 por el CRLR+RAMP2 y el AM2 por el CRLR+RAMP3. A nivel del sistema nervioso central, la AM y sus receptores se localizan en diversas regiones, incluyendo el cerebelo. Se ha demostrado marcados incrementos en la densidad de los sitios de unión para la AM en el cerebelo durante la hipertensión, lo que sugiere un papel del sistema adrenomedulinérgico cerebeloso en la regulación de la presión arterial (PA). En el presente estudio se evaluó la participación de la AM cerebelosa en la regulación de la PA. Nuestros hallazgos muestran la existencia de desregulación de los componentes del sistema AM cerebeloso durante la hipertensión, ya que se encontró una reducida expresión de CRLR, RAMP1 y RAMP3 y una incrementada expresión de la AM y RAMP2 en el vermis de cerebelo de ratas hipertensas (SHR), cuando se comparó con las ratas controles, Wistar Kyoto (WKY), de 8 y 16 semanas de edad. La reducción de la PA mediante el tratamiento crónico con valsartán (60mg/Kg/día,p.o.) revirtió las desregulación de la AM y los componentes de su receptor, observados en las ratas SHR. El papel de las especies reactivas de oxígeno (EROS) en la acción de la AM cerebelosa quedó evidenciado, ya que la AM fue capaz de reducir la actividad de las tres enzimas antioxidantes, superóxiodo dismutasa (SOD), catalasa (CAT) y glutatión peroxidasa (GPx), en las ratas WKY y Sprague - Dawley (SD). Aún mas, nuestros hallazgos mostraron claramente el efecto antagónico entre la AM y la ANG II sobre la actividad de las enzimas antioxidantes inducida por la ANG II. El efecto de la AM sobre las enzimas antioxidantes no se manifestó en la ratas hipertensas, sin embargo el mismo fue restaurado mediante la disminución de la presión arterial con la administración crónica de dos antihipertensivos de mecanismo de acción distintos como la amlodipina (5mg/Kg/día,p.o) o el valsartán (60mg/Kg/día,p.o), lo que sugiere una relación entre la hipertensión y ausencia del efecto de AM en la ratas SHR. Al evaluar la posible vía de señalización que media la acción de la AM y el antagonismo con la ANG II sobre la actividad de las enzimas antioxidantes, demostramos que no existe una vía final común para dicho antagonismo, siendo la proteína quinasa A (PKA) y los 3 subtipos de receptores CGRP1, AM1 y AM2 los que median la acción de la AM, mientras que la acción de la ANG II se encuentra mediada a través de una vía que involucra la PKC/NAD(P)H oxidasa. Los hallazgos demuestran el antagonismo entre la AM y la ANG II en la regulación del estrés oxidativo en el cerebelo y ratifican la desregulación de la señalización de la AM mediada por EROs durante la hipertensión. Al evaluar las vías de señalización intracelular que median la acción de la AM en el cerebelo, demostramos que la AM es capaz de activar a las ERK, la producción de GMPc y NO a través de la estimulación del receptor AM1, y del AMPc a través de los tres subtipos de receptores de AM, lo que apoya que en el cerebelo la AM ejerce acciones a través de diversas vías de señalización como lo son NO/GMPc, AC/AMPc/PKA y/o ERK. El posible papel funcional de la AM in vivo fue inequívoco, ya que se demostró que la microinyección de AM en el vermis cerebeloso produjo una respuesta hipotensora profunda en las ratas SHR pero no en las normotensas. El hecho que la microinyección de AM en el vermis cerebelar en las ratas SD, WKY y SHR disminuyó significativamente la respuesta presora frente al estrés simpatoadrenal inducido por el estímulo eléctrico plantar, sugiere que la acción hipotensora está mediada a través de la regulación del eflujo simpático e indica un posible papel de la AM en la regulación de la respuesta CDV frente al estrés. En conjunto, nuestros resultados demuestran la existencia de un sistema adrenomedulinérgico funcional en el cerebelo, e indican por primera vez, que la AM cumple un papel importante en la regulación de la PA durante la hipertensión y el estrés.
Descritores: Adrenomedulina/metabolismo
Pressão Arterial/fisiologia
Vermis Cerebelar/metabolismo
Hipertensão/metabolismo
-Ratos Endogâmicos SHR
Ratos Endogâmicos WKY
Fatores de Tempo
Vasoconstritores/administração & dosagem
Angiotensina II/administração & dosagem
Western Blotting
Ratos Sprague-Dawley
Anlodipino/administração & dosagem
Estresse Oxidativo
Modelos Animais
Adrenomedulina/administração & dosagem
Receptores de Adrenomedulina/metabolismo
Pressão Arterial/efeitos dos fármacos
Vermis Cerebelar/efeitos dos fármacos
Vermis Cerebelar/enzimologia
Valsartana/administração & dosagem
Anti-Hipertensivos/administração & dosagem
Limites: Animais
Masculino
Ratos
Responsável: VE497.1 - Biblioteca Dr. Oswaldo Enríquez Isava
VE497.1; D-CF, F53


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Id: biblio-1137269
Autor: Kirisci, Mehmet; Gunes, Hakan; Kocarslan, Aydemir; Metin, Tuba Ozcan; Aykan, Duygun Altintas; Seyithanoglu, Muhammed; Doganer, Adem; Bayrak, Gulsen; Aksu, Ekrem.
Título: Protective effects of adrenomedullin on rat cerebral tissue after transient bilateral common carotid artery occlusion and reperfusion
Fonte: Rev. bras. cir. cardiovasc = Braz. j. cardiovasc. surg. (impr.);35(3):314-322, May-June 2020. tab, graf.
Idioma: en.
Resumo: Abstract Objective: We aimed to investigate the protective effect of adrenomedullin (ADM) on cerebral tissue of rats with cerebral ischemia/reperfusion (I/R) injury. Methods: Thirty-two Wistar rats were randomized into four groups (n=8). In the I/R Group, bilateral common carotid arteries were clamped for 30 minutes and, subsequently, reperfused for 120 minutes. In the ADM Group, rats received 12 µg/kg of ADM. In the I/R+ADM Group, bilateral common carotid arteries were clamped for 30 minutes and, subsequently, the rats received 12 µg/ kg of ADM. Then, reperfusion was performed for 120 minutes. The Control Group underwent no procedure. Blood and brain tissue samples were collected for biochemical and histopathological analysis. Serum malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were analysed. Brain tissue was evaluated histopathologically and neuronal cells were counted in five different fields, at a magnification of ×400. Results: Brain MDA in I/R Group was significantly higher than in ADM Group. Brain GPx and SOD in I/R+ADM Group were significantly higher than in I/R Group. The number of neurons was decreased in I/R Group compared to the Control Group. The number of neurons in I/R+ADM Group was significantly higher than in I/R Group, and lower than in Control Group. Apoptotic changes decreased significantly in I/R+ADM Group and the cell structure was similar in morphology compared to the Control Group. Conclusion: We demonstrated the cerebral protective effect of ADM in the rat model of cerebral I/R injury after bilateral carotid artery occlusion.
Descritores: Artéria Carótida Primitiva
-Reperfusão
Traumatismo por Reperfusão/prevenção & controle
Ratos Wistar
Adrenomedulina
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


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Id: lil-751375
Autor: Lee, Dong-Kyu; Kang, Min-Hyeok; Lee, Tae-Sik; Oh, Jae-Seop.
Título: Relationships among the Y balance test, Berg Balance Scale, and lower limb strength in middle-aged and older females
Fonte: Braz. j. phys. ther. (Impr.) = Rev. bras. fisioter;19(3):227-234, May-Jun/2015. tab, graf.
Idioma: en.
Resumo: Background: Older females have less dynamic postural control and muscle strength than do middle-aged females. Aging-related strength losses may limit balancing performance. Objective: The purpose of this study was to investigate the ability of the Y Balance Test (YBT) and lower limb strength to discriminate between females in 2 age groups, the relationship between YBT distance and the Berg Balance Scale (BBS), and the degree to which performance on YBT distance is related to lower limb strength in middle-aged and older females. Method: The 40 healthy, independently active females were divided into 2 groups: older and middle-aged. The participants underwent measurements of YBT distance using the YBT, maximal muscular strength of the lower limbs using a handheld dynamometer, and the BBS. Results: The YBT distance in 3 directions and lower limb muscle strength for both lower limbs were significantly lower in the older adults than in the middle-aged group. A moderate correlation but insignificant correlation was found between the YBT composite distance and the BBS score. In the older females, YBT distance was significantly positively correlated with strength of the knee flexor and hip abductor. In the middle-aged group, YBT distance was significantly positively correlated with strength of the knee flexor and hip extensor. Conclusions: Performance on the YBT was influenced by the strength of lower limb. We suggested that YBT can be used to alternative as a measurement of dynamic balance. Proper training programs for older people could include not only strengthening exercises but also YBT performance to improve balance. .
Descritores: Adrenomedulina/metabolismo
Neoplasias Colorretais/genética
Neoplasias Colorretais/metabolismo
Proteínas Proto-Oncogênicas/genética
Proteínas ras/genética
-Linhagem Celular Tumoral
Hipóxia Celular/fisiologia
Imuno-Histoquímica
Camundongos Nus
Análise de Sequência com Séries de Oligonucleotídeos
Microambiente Tumoral/fisiologia
Ensaios Antitumorais Modelo de Xenoenxerto
Limites: Animais
Feminino
Humanos
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-722165
Autor: Leite, L.N.; Gonzaga, N.A.; Tirapelli, D.P.C.; Tirapelli, L.F.; Tirapelli, C.R..
Título: Pharmacological characterization of the relaxant effect induced by adrenomedullin in rat cavernosal smooth muscle
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(10):876-885, 10/2014. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: The aim of the present study was to determine the mechanisms underlying the relaxant effect of adrenomedullin (AM) in rat cavernosal smooth muscle (CSM) and the expression of AM system components in this tissue. Functional assays using standard muscle bath procedures were performed in CSM isolated from male Wistar rats. Protein and mRNA levels of pre-pro-AM, calcitonin receptor-like receptor (CRLR), and Subtypes 1, 2 and 3 of the receptor activity-modifying protein (RAMP) family were assessed by Western immunoblotting and quantitative real-time polymerase chain reaction, respectively. Nitrate and 6-keto-prostaglandin F1α (6-keto-PGF1α; a stable product of prostacyclin) levels were determined using commercially available kits. Protein and mRNA of AM, CRLR, and RAMP 1, -2, and -3 were detected in rat CSM. Immunohistochemical assays demonstrated that AM and CRLR were expressed in rat CSM. AM relaxed CSM strips in a concentration-dependent manner. AM22-52, a selective antagonist for AM receptors, reduced the relaxation induced by AM. Conversely, CGRP8-37, a selective antagonist for calcitonin gene-related peptide receptors, did not affect AM-induced relaxation. Preincubation of CSM strips with NG-nitro-L-arginine-methyl-ester (L-NAME, nitric oxide synthase inhibitor), 1H-(1,2,4)oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, quanylyl cyclase inhibitor), Rp-8-Br-PET-cGMPS (cGMP-dependent protein kinase inhibitor), SC560 [5-(4-chlorophenyl)-1-(4-methoxyphenyl)-3-trifluoromethyl pyrazole, selective cyclooxygenase-1 inhibitor], and 4-aminopyridine (voltage-dependent K+ channel blocker) reduced AM-induced relaxation. On the other hand, 7-nitroindazole (selective neuronal nitric oxide synthase inhibitor), wortmannin (phosphatidylinositol 3-kinase inhibitor), H89 (protein kinase A inhibitor), SQ22536 [9-(tetrahydro-2-furanyl)-9H-purin-6-amine, adenylate cyclase inhibitor], glibenclamide (selective blocker of ATP-sensitive K+ channels), and apamin (Ca2+-activated channel blocker) did not affect AM-induced relaxation. AM increased nitrate levels and 6-keto-PGF1α in rat CSM. The major new contribution of this research is that it demonstrated expression of AM and its receptor in rat CSM. Moreover, we provided evidence that AM-induced relaxation in this tissue is mediated by AM receptors by a mechanism that involves the nitric oxide-cGMP pathway, a vasodilator prostanoid, and the opening of voltage-dependent K+ channels.
Descritores: Adrenomedulina/farmacologia
Proteína Semelhante a Receptor de Calcitonina/análise
Músculo Liso/efeitos dos fármacos
Parassimpatolíticos/farmacologia
Pênis/efeitos dos fármacos
Vasodilatadores/farmacologia
-/farmacologia
ABBREVIATIONS AS TOPIC-AMINOPYRIDINE/farmacologia
/análise
ABDOMEN, ACUTE-KETOPROSTAGLANDIN F1 ALPHA/análise
Adrenomedulina/genética
Adrenomedulina/metabolismo
Western Blotting
Proteína Semelhante a Receptor de Calcitonina/antagonistas & inibidores
Proteínas Quinases Dependentes de GMP Cíclico/antagonistas & inibidores
Inibidores de Ciclo-Oxigenase/farmacologia
Relação Dose-Resposta a Droga
Inibidores Enzimáticos/farmacologia
Imuno-Histoquímica
Indazóis/farmacologia
Relaxamento Muscular
Músculo Liso/metabolismo
Óxido Nítrico Sintase/antagonistas & inibidores
Óxido Nítrico/análise
Óxido Nítrico/análogos & derivados
Pênis/metabolismo
Canais de Potássio de Abertura Dependente da Tensão da Membrana/metabolismo
Ratos Wistar
Reação em Cadeia da Polimerase em Tempo Real
RNA Mensageiro/metabolismo
Proteína 1 Modificadora da Atividade de Receptores/genética
Proteína 1 Modificadora da Atividade de Receptores/metabolismo
/metabolismo
RECEPTOR ACTIVITY-MODIFYING PROTEIN ABATTOIRS/metabolismo
/genética
RECEPTOR ACTIVITY-MODIFYING PROTEIN TEMEFOS/genética
/metabolismo
RECEPTOR ACTIVITY-MODIFYING PROTEIN TEMEFOS/metabolismo
Receptores de Peptídeo Relacionado com o Gene de Calcitonina/metabolismo
Limites: Animais
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Yamamoto, Lidia
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Id: lil-665923
Autor: Arkader, Ronaldo; Malbouisson, Luiz Marcelo; Del Negro, Gilda Maria Barbaro; Yamamoto, Lidia; Okay, Thelma Suely.
Título: Factors associated with hyperglycemia and low insulin levels in children undergoing cardiac surgery with cardiopulmonary bypass who received a single high dose of methylprednisolone
Fonte: Clinics;68(1):85-92, Jan. 2013. graf, tab.
Idioma: en.
Resumo: OBJECTIVES: Administering steroids before cardiopulmonary bypass in pediatric heart surgery modulates systemic inflammatory response syndrome and improves postoperative recovery. However, the use of steroids aggravates hyperglycemia, which is associated with a poor prognosis. Adult patients with systemic inflammatory response syndrome usually evolve with hyperglycemia and high insulin levels, whereas >90% of pediatric patients exhibit hyperglycemia and low insulin levels. This study aims to determine: A) the metabolic and inflammatory factors that are associated with hyperglycemia and low insulin levels in children who underwent cardiac surgery with cardiopulmonary bypass and who received a single high dose of methylprednisolone and B) the best predictors of insulin variation using a mathematical model. METHODS: This preliminary study recruited 20 children who underwent heart surgery with cardiopulmonary bypass and received methylprednisolone (30 mg/kg) immediately after anesthesia. Among the 20 patients initially recruited, one was excluded because of the absence of hyperglycemia and lower insulin levels after surgery. However, these abnormalities were confirmed in the remaining 19 children. The C-peptide, CRP, IL-6, and adrenomedullin levels were measured before surgery, immediately after cardiopulmonary bypass, and on the first, second, and third days after cardiac surgery. RESULTS: IL-6, CRP, and adrenomedullin increments were observed, whereas the C-peptide levels remained within reference intervals. CONCLUSION: The multiple regression model demonstrated that in addition to age and glycemia (two well-known factors that are directly involved in glucose metabolism), adrenomedullin and IL-6 levels were independent factors associated with lower insulin concentrations. These four parameters were responsible for 64.7% of the observed insulin variances. In addition, the fact that C-peptide levels did not fall together with insulin could have grounded the medical decision not to administer insulin to patients.
Descritores: Anti-Inflamatórios/efeitos adversos
Procedimentos Cirúrgicos Cardíacos/métodos
Ponte Cardiopulmonar/métodos
Hiperglicemia/induzido quimicamente
Insulina/sangue
Metilprednisolona/efeitos adversos
-Fatores Etários
Adrenomedulina/sangue
Anti-Inflamatórios/administração & dosagem
Glicemia/análise
Glicemia/efeitos dos fármacos
Peptídeo C/sangue
Proteína C-Reativa/análise
Insulina/deficiência
/sangue
INTERLEUKIN-ABDOMEN, ACUTE/sangue
Modelos Biológicos
Metilprednisolona/administração & dosagem
Período Pós-Operatório
Valores de Referência
Análise de Regressão
Limites: Pré-Escolar
Feminino
Humanos
Lactente
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-618045
Autor: Cam-Etoz, B; Isbil-Buyukcoskun, N; Ozluk, K.
Título: Cardiovascular effects of the intracerebroventricular injection of adrenomedullin: roles of the peripheral vasopressin and central cholinergic systems
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;45(3):250-255, Mar. 2012. ilus.
Idioma: en.
Resumo: Our objective was to investigate in conscious Sprague-Dawley (6-8 weeks, 250-300 g) female rats (N = 7 in each group) the effects of intracerebroventricularly (icv) injected adrenomedullin (ADM) on blood pressure and heart rate (HR), and to determine if ADM and calcitonin gene-related peptide (CGRP) receptors, peripheral V1 receptors or the central cholinergic system play roles in these cardiovascular effects. Blood pressure and HR were observed before and for 30 min following drug injections. The following results were obtained: 1) icv ADM (750 ng/10 µL) caused an increase in both blood pressure and HR (DMAP = 11.8 ± 2.3 mmHg and ΔHR = 39.7 ± 4.8 bpm). 2) Pretreatment with a CGRP receptor antagonist (CGRP8-37) and ADM receptor antagonist (ADM22-52) blocked the effect of central ADM on blood pressure and HR. 3) The nicotinic receptor antagonist mecamylamine (25 µg/10 µL, icv) and the muscarinic receptor antagonist atropine (5 µg/10 µL, icv) prevented the stimulating effect of ADM on blood pressure. The effect of ADM on HR was blocked only by atropine (5 µg/10 µL, icv). 4) The V1 receptor antagonist [β-mercapto-β-β-cyclopentamethylenepropionyl¹, O-me-Tyr²,Arg8]-vasopressin (V2255; 10 µg/kg), that was applied intravenously, prevented the effect of ADM on blood pressure and HR. This is the first study reporting the role of specific ADM and CGRP receptors, especially the role of nicotinic and muscarinic central cholinergic receptors and the role of peripheral V1 receptors in the increasing effects of icv ADM on blood pressure and HR.
Descritores: Adrenomedulina/farmacologia
Pressão Sanguínea/efeitos dos fármacos
Neurônios Colinérgicos/fisiologia
Frequência Cardíaca/efeitos dos fármacos
Vasodilatadores/farmacologia
Vasopressinas/efeitos dos fármacos
-Adrenomedulina/administração & dosagem
Sistema Nervoso Central/efeitos dos fármacos
Sistema Nervoso Central/fisiologia
Neurônios Colinérgicos/efeitos dos fármacos
Estado de Consciência/efeitos dos fármacos
Estado de Consciência/fisiologia
Injeções Intraventriculares
Ratos Sprague-Dawley
Receptores de Peptídeo Relacionado com o Gene de Calcitonina/efeitos dos fármacos
Receptores de Peptídeo Relacionado com o Gene de Calcitonina/fisiologia
Vasodilatadores/administração & dosagem
Vasopressinas/fisiologia
Limites: Animais
Feminino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-601917
Autor: Karatepe, Oguzhan; Kurtulus, Idris; Yalcin, Orhan; Battal, Muharrem; Kamali, Gulcin; Aydin, Timucin.
Título: Adrenomedulline improves ischemic left colonic anastomotic healing in an experimental rodent model
Fonte: Clinics;66(10):1805-1810, 2011. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Leakage from colonic anastomosis is a major complication causing increased mortality and morbidity. Ischemia is a well-known cause of this event. This study was designed to investigate the effects of adrenomedullin on the healing of ischemic colon anastomosis in a rat model. METHODS: Standardized left colon resection 3 cm above the peritoneal reflection and colonic anastomosis were performed in 40 Wistar rats that were divided into four groups. To mimic ischemia, the mesocolon was ligated 2 cm from either side of the anastomosis in all of the groups. The control groups (1 and 2) received no further treatment. The experimental groups (3 and 4) received adrenomedullin treatment. Adrenomedullin therapy was started in the perioperative period in group 3 and 4 rats (the therapeutic groups). Group 1 and group 3 rats were sacrificed on postoperative day 3. Group 2 and group 4 rats were sacrificed on postoperative day 7. After careful relaparotomy, bursting pressure, hydroxyproline, malondialdehyde, interleukin 6, nitric oxide, vascular endothelial growth factor, and tumor necrosis factor alpha levels were measured. Histopathological characteristics of the anastomosis were analyzed. RESULTS: The group 3 animals had a significantly higher bursting pressure than group 1 (p<0.05). Hydroxyproline levels in group 1 were significantly lower than in group 3 (p<0.05). The mean bursting pressure was significantly different between group 2 and group 4 (p<0.05). Hydroxyproline levels in groups 3 and 4 were significantly increased by adrenomedullin therapy relative to the control groups (p<0.05). When all groups were compared, malondialdehyde and nitric oxide were significantly lower in the control groups (p<0.05). When vascular endothelial growth factor levels were compared, no statistically significant difference between groups was observed. Interleukin 6 and tumor necrosis factor alpha were significantly decreased by adrenomedullin therapy (p<0.05). The healing parameters and inflammatory changes (e.g., granulocytic cell infiltration, necrosis, and exudate) were significantly different among all groups (p<0.05). CONCLUSION: Adrenomedullin had positive effects on histopathologic anastomotic healing in this experimental model of ischemic colon anastomosis.
Descritores: Adrenomedulina/uso terapêutico
Colo/irrigação sanguínea
Colo/cirurgia
Isquemia/tratamento farmacológico
Vasodilatadores/uso terapêutico
Cicatrização/efeitos dos fármacos
-Anastomose Cirúrgica
Fístula Anastomótica/tratamento farmacológico
Modelos Animais de Doenças
Isquemia/patologia
Período Pós-Operatório
Complicações Pós-Operatórias/prevenção & controle
Ratos Wistar
Fatores de Tempo
Cicatrização/fisiologia
Limites: Animais
Feminino
Ratos
Responsável: BR1.1 - BIREME


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Id: lil-516927
Autor: Pastorello, Mariella; Díaz, Emilia; Csibi, Alfredo; Garrido, María del Rosario; Chabot, Jean Guy; Quirion, Rémie; Israel, Anita.
Título: Papel de la adrenomedulina cerebelosa en la hipertensión arterial / Paper of the adrenomedulina cerebelosa in the arterial hypertension
Fonte: Arch. venez. farmacol. ter;26(2):98-104, 2007. ilus, graf.
Idioma: es.
Resumo: La adrenomedulina (AM) y el péptido relacionado con el gen de la calcitonina (CGRP) pertenecen a la superfamilia de los péptidos de CGRP. En el SNC, los sitios de unión para la AM y el CGRP se encuentran presentes en áreas hipotalámicas y en la corteza cerebelosa de la rata. La administración central de AM o de CGRP en ratas induce diuresis, natriuresis e incremento de la presión arterial. El papel de la AM en el cerebelo se desconoce. Con el fin de establecer la posible relación de la AM y CGRP cerebelosa y la regulación cardiovascular, en el presente estudio evaluamos la densidad de sitios de unión para la AM y el CGRP en el cerebelo de ratas espontáneamente hipertensas (SHR) y sus controles normotensos Wistar Kyoto (WKY) adultos de 16 semanas, mediante el uso de técnicas autoradiografícas y empleando 125I-hCGRPα y 125I-hAM13-52 como radioligandos. Los cortes coronales de cerebelo fueron incubados con 35 pM de [125I]-hCGRPα o [125I]-hAM13-52, durante 90 y 120 minutos, respectivamente. La unión no específica fue determinada en presencia de 1µM del ligando no marcado. El análisis densitométrico demostró que existe una colocalización de los sitios de unión para el [125I]-hCGRPα y la [125I]-hAM13-52 en la corteza cerebelosa. En el cerebelo la unión de la [125I]-hAM13-52 en las ratas SHR fue significativamente mayor que las WKY, indicando una mayor expresión de los receptores para la AM en el cerebelo de animales hipertensos. En relación a la unión de [125I]-hCGRPα, se observó también un pequeño incremento significativo en las ratas SHR en relación a las WKY. Con el fin de establecer la posible vía de señalización de la AM en la corteza cerebelosa, se evaluó la actividad de la óxido nítrico sintasa inducida por la AM.
Descritores: Adrenomedulina/fisiologia
Cerebelo/fisiologia
Hipertensão/fisiopatologia
Óxido Nítrico/metabolismo
Peptídeo Relacionado com Gene de Calcitonina/fisiologia
-Modelos Animais de Doenças
Ratos Sprague-Dawley
Receptores de Peptídeo Relacionado com o Gene de Calcitonina/fisiologia
Limites: Masculino
Animais
Ratos
Tipo de Publ: Revisão
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Id: lil-509839
Autor: Arkader, Ronaldo.
Título: Concentrações de adrenomedulina e relações com o eixo hipófise-adrenal e o controle glicêmico de crianças submetidas à cirurgia cardíaca com circulação extracorpórea / Adrenomedullin concentration and relationship with pituitayadrenal axis and glicemic control in children after cardiopulmonary bypass.
Fonte: São Paulo; s.n; 2008. [114] p. ilus, tab, graf.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Medicina. Departamento de Pediatria para obtenção do grau de Doutor.
Resumo: Introdução: Adrenomedulina, peptídeo recentemente identificado, atua como mediador em diversos estados fisiológicos e patológicos, sendo expresso em inúmeros tecidos. Níveis elevados de adrenomedulina foram observados em quadros inflamatórios e sépticos, além de atividade inibitória da adrenomedulina sobre a liberação de insulina, o que poderia contribuir para a gênese e manutenção dos quadros hipeglicêmicos, situação comum em pacientes pediátricos internados em unidades de terapia intensiva, tendo sido comprovado que o controle glicêmico melhora o prognóstico nesses pacientes. O objetivo do estudo foi relacionar as concentrações de adrenomedulina com alterações de hormônios do eixo hipófise-adrenal e o controle glicêmico de crianças submetidas à cirurgia cardíaca com circulação extracorpórea (CEC) representando modelo de síndrome da resposta inflamatória sistêmica. Material e métodos: Foram incluídas 20 crianças com idade entre 11-84 meses submetidas à cirurgia cardíaca com CEC. Foram avaliadas concentrações sangüíneas de glicose, insulina, peptídeo-C, cortisol, ACTH, interleucina-6 e adrenomedulina nos tempos pré-CEC, pós-CEC, 1ºPO, 2ºPO e 3ºPO. Os resultados foram expressos em medianas e inter-quartis. Todas crianças receberam antibioticoterapia profilática (Cefuroxima 150mg/kg) e metilprednisolona (30mg/kg) na indução anestésica. Resultados: Adrenomedulina aumentou de 1,13 ng/mL (0.83 1.81) pré- CEC a 3,28 ng/mL (1.82 4.25) no 1ºPO (p=0,0005). A insulina e o peptídeo-C apresentaram queda do tempo pré-CEC ao 1ºPO: 10,9 U/mL (10.5 13.6) a 2,88 U/mL (1.68 3.46) (p= 0,0001) e 1,44 (0.93 2.08) a 0.89 ng/mL (0.58 2.06) (p=0,076), respectivamente. Houve aumento da glicemia de 86,5 mg/dL (67 88) pré-CEC a 169 mg/dL (153 201) no 1ºPO (p=0,0001). Não houve alterações significativas das concentrações de cortisol e ACTH. Após a CEC todas as concentrações de interleucina-6 encontravam-se elevadas retornando aos valores normais no 3ºPO...

Background: Adrenomedullin (AM), a recently identified vasoactive peptide, is expressed in a large number of tissues and was found to be increased in some pathophysiologic conditions such as sepsis and Systemic Inflammatory Response Syndrome (SIRS). It has been shown that AM inhibits insulin secretion by means of a direct action on pancreatic -cells thus contributing to the genesis and sustaining of hypeglicemia, a common condition in critically ill pediatric patients. Therefore, the strict control of glucose concentrations has proved beneficial and associated to decrement of morbimortality rates in adults and children. The aim of the present study was to monitor serum adrenomedullin concentration in parallel with interleukin-6, plasma glucose, insulin and C-peptide, as well as ACTH and cortisol in pediatric patients submitted to cardiac surgery and cardiopulmonary bypass (CPB) representing a SIRS model. Methods: Twenty children aged 11-84 months who underwent cardiac surgery requiring cardiopulmonary bypass were prospective studied. Blood glucose, insulin, C-peptide, adrenocorticotropic hormone, cortisol, interleukin- 6 and AM were measured immediately before cardiopulmonary bypass (BCPB), immediately after CPB, on the first (POD1), second (POD2) and third days (POD3) after surgery. Data were expressed by median values and inter quartile range. All children received profilatic antibiotic (cefuroxime 150mg/kg) and methylprednisolone (30mg/kg) during induction of anesthesia. Results: Adrenomedullin increased from 1.13 ng/mL (0.83 1.81) BCPB to 3.28 ng/mL (1.82 4.25) in POD1 (p=0.0005). Insulin dropped from 10.9 U/mL (10.5 13.6) BCPB to 2.88 U/mL (1.68 3.46) in POD1 (p=0.0000). C-peptide decreased from 1.44 (0.93 2.08) BCPB to 0.89 ng/mL (0.58 2.06) in POD1 (p=0.076). Glucose increased from 86.5 mg/dL (67 88) BCPB to 169 mg/dL (153 201) in POD1 (p=0.0000). No significant changes were observed in ACTH and cortisol levels...
Descritores: Adrenomedulina
Criança
Circulação Extracorpórea
Insulina
Sistema Hipófise-Suprarrenal
-Glicemia
Cirurgia Torácica
Limites: Humanos
Masculino
Feminino
Criança
Responsável: BR66.1 - Divisão de Biblioteca e Documentação
BR66.1; W4.DB8, A795co, FM-2, 2008


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Id: lil-482040
Autor: Pérez, I. A; Santana, S. P; Argudin, T. D; Gardon, D. O.
Título: Analysis of blood processing conditions to obtain high-quality total RNA from human leukocyte concentrate
Fonte: Genet. mol. res. (Online);6(2):298-307, 2007. tab, graf.
Idioma: en.
Resumo: Blood samples are used as a biological source to discover biomarkers of hematological and non-hematological disorders. The present study shows the impact of different experimental conditions associated with cell lysis buffer, TRI-reagent protocol and blood cell storage buffer and their correlation with the quantity, quality and Adrenomedullin gene expression levels of total RNA when RT-PCR technique is used. A leukocyte cell bank protocol is also proposed for further mRNA expression analysis using RNAlater as storage buffer. There is evidence that total RNA isolated from leukocyte concentrate stored for 1 month at -70 degrees C did not show significant differences concerning quality, purity and Adrenomedullin gene expression compared with the freshly processed leukocyte sample.
Descritores: Preservação de Sangue
Leucócitos/química
RNA
RNA Mensageiro/análise
-Adrenomedulina/genética
Tampões (Química)
Perfilação da Expressão Gênica
Análise de Sequência com Séries de Oligonucleotídeos
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limites: Humanos
Responsável: BR1.1 - BIREME



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